113 results about "Preparing skin" patented technology

The present invention relates to the technical field of new drug indications, and specifically discloses a non-filling breast augmentation external skin-rejuvenating oil preparation and its preparation method and application. The preparation method of the external skin-rejuvenating oil preparation is as follows: (1) preparing a skin-rejuvenating oil alcohol extract freeze-dried powder: taking astragalus, angelica, epimedium, ginkgo, green peel, frankincense, rhubarb, chicken gizzard, scutellaria, coptis, phellodendron and borneol in a certain weight ratio and adding them together into a container, and then adding an ethanol solution with a volume fraction of 40%-70% to the container, soaking, flash extraction, filtrate concentration, freeze drying to obtain; (2) preparing a skin-rejuvenating oil preparation: dissolving the skin-rejuvenating oil alcohol extract freeze-dried powder and egg yolk lecithin in edible vegetable oil to obtain an oil phase; then dripping the oil phase into the water phase obtained by dissolving Tween 60 and glycerol in ultrapure water, stirring and high-speed shearing, high-pressure homogenization, and obtaining. The external skin-rejuvenating oil preparation of the present invention has the effect of promoting breast fullness and uprightness in a short period of time.

The invention provides a tubulin-SRC double-target inhibitor in a crystal form as well as a preparation method and application thereof, and particularly discloses 13 crystal forms of two free crystal forms and 10 salts of a compound as shown in a formula I as well as a preparation method and application thereof. The compound, the salt form and the crystal form can be used as a tubulin and Src kinase double-target inhibitor, and can also be used as a single tubulin or Src kinase inhibitor; the compound, the salt form and the crystal form can also obviously inhibit polymerization of tubulin monomers and inhibit cell proliferation; the compound has good pharmacokinetic properties of skin local administration and good druggability, can be used for preparing skin external preparations, and has the advantages of fast metabolism and small side effects. # imgabs0 #

The present invention discloses an application of LCE5A as a target in the preparation of a drug for treating inflammatory skin diseases including psoriasis, wherein the LCE5A is mainly expressed on the epidermis; the present invention confirms through a series of experiments that LCE5A can effectively alleviate imiquimod-induced psoriasis-like skin inflammation in mice. The protein can significantly improve the barrier function of mouse skin, inhibit the release of proinflammatory factors and reduce the infiltration of inflammatory cells by regulating the proliferation and differentiation of keratinocytes. This provides important experimental support for the use of LCE5A in the preparation of drugs for skin diseases caused by skin barrier damage, as well as the development of functional skin care products. The experimental results clearly show that LCE5A exhibits significant therapeutic effects in a mouse psoriasis-like inflammation model, and its effect is safe and stable. The protein has a simple extraction and purification process, has good industrial production potential, and lays a solid foundation for clinical application.

The invention discloses a soothing and oil-controlling composition derived from natural plants and application thereof, and belongs to the technical field of daily cosmetics. The composition is formed by compounding an artemisia annua extract, a rehmannia root extract, a calendula extract and a pomelo fruit extract, and the four plant extracts have a synergistic effect, have the effects of repairing, relieving and controlling oil, and can be used for preparing a skin care product special for oil-sensitive skin. The composition provided by the invention can effectively solve the problems of external oil and internal dry, redness and sensitivity, grease accumulation and the like of oily sensitive skin, and meets multiple skin care requirements.

The invention relates to a micromolecule RNA (Ribonucleic Acid) composition and an application thereof in preparing a skin beautifying and repairing product. Comprising tiRNA-1: 34-Glu-CTC-1-M2 and tRF-1: 30-Glu-CTC-1-M2, and the nucleotide sequences of the tiRNA-1: 34-Glu-CTC-1-M2 and the tRF-1: 30-Glu-CTC-1-M2 are as shown in SEQ ID The invention further provides application of the small molecule RNA composition in preparation of skin beautifying and repairing products. It is found for the first time that the composition of tiRNA-1: 34-Glu-CTC-1-M2 and tRF-1: 30-Glu-CTC-1-M2 can promote proliferation and migration of human skin cells, collagen synthesis and secretion in the human skin cells and expression of genes related to angiogenesis are improved, and meanwhile aging of the human skin cells is delayed. Besides, the small molecule nucleic acid RNA composition reduces expression of inflammation-related factors so as to exert an anti-inflammatory effect, exerts the effects of promoting skin regeneration and repair, improving skin aging, resisting inflammation, promoting skin collagen and blood vessel regeneration and the like, can be used for preparing skin beautifying and repairing products, provides a new method and a new thought for the field of skin beauty treatment, and has wide application prospects. Good commercial application prospects are realized.

The invention belongs to the technical field of cosmetics, particularly discloses moisturizing nano-liposome emulsion prepared by a microjet method and a preparation method of the moisturizing nano-liposome emulsion, and aims to solve the problems of poor stability of active ingredients, low transdermal absorption efficiency, single effect and non-uniform particle size in a liposome preparation process in the existing moisturizing cosmetics. The nano-liposome emulsion is prepared from glycosylglycerol, acetylchitosamine, tremella polysaccharide, a myrothamnus flabellifolia leaf / stem extract, an artemisia annua extract, a lactobacillus fermentation product, flat nuclear wood oil, hydrogenated lecithin and phosphatidylcholine. The emulsion has excellent moisturizing, soothing and repairing effects, can be used for preparing skin care products such as water aqua, emulsion and cream, and has the advantages of high utilization rate of active ingredients and good stability.

The invention provides application of manuka honey in skin antibiosis and anti-inflammation, and belongs to the technical field of cosmetics, the application comprises the following steps: (1) mixing rose petals, perilla roots, grape seeds and manuka honey, and adding the mixture into water to obtain an enzymolysis substrate; (2) sequentially adding pectinase, neutral protease and beta-glucanase into the enzymolysis substrate, and performing enzymolysis to obtain an enzymolysis product; (3) adding lactobacillus brevis, lactobacillus helveticus and bifidobacterium lactis into the enzymolysis product, and fermenting to obtain a fermentation product; (4) adding an ethanol aqueous solution into the fermentation product, stirring, filtering by using a filter membrane to obtain a supernatant, and concentrating the supernatant to obtain a mixed extract; and (5) preparing skin antibacterial and anti-inflammatory products from the mixed extract. The mixed extract disclosed by the invention is good in batch stability, relatively good in antibacterial and anti-inflammatory effects and low in irritation.

The application provides a copper peptide efficient soothing composition and application thereof, which comprises copper peptide and symbiotic fermentation products of thermus thermophilus and bacillus, can target stimulation of secretion of inflammatory mediators, effectively inhibits secretion of PGE2 and expression of inflammatory factors IL-6, TNF alpha and NO, and has a synergistic effect, especially on secretion of PGE2 and expression of NO, and shows a more significant soothing effect. The composition is used as an efficacy component to prepare skin care products, is particularly suitable for people with sensitive skin, can help the skin to restore a healthy state, and has a good application prospect.

The invention provides 3D printing bio-ink as well as a preparation method and application thereof. The 3D printing bio-ink is prepared from the following components: methacrylic anhydride gelatin, oxidized hyaluronic acid, carboxymethyl chitosan and 2-methacryloyloxyethyl phosphorylcholine. By designing the specific composition of the 3D printing bio-ink, the 3D printing bio-ink which is good in printing performance, mechanical strength and electrical conductivity and high in cell survival rate after printing is prepared, and then a 3D printing stent with high cell-stent interaction is prepared and can be used for preparing a skin injury repair material.

The invention discloses application of o-tolyl biguanide in preparation of a skin whitening product, the skin whitening product and a preparation method, and belongs to the technical field of skin care. It is found for the first time that o-tolyl biguanide can effectively inhibit generation of advanced glycosylation end products, namely carboxy methyl lysine (CML), lipofuscin and melanin when serving as a whitening active ingredient, and therefore the multi-channel whitening effect is achieved. The invention further provides a whitening product, the whitening product comprises the o-tolyl biguanide and the anionic surfactant which are similar in charge quantity, the o-tolyl biguanide is positively charged, the anionic surfactant is negatively charged, and the o-tolyl biguanide and the anionic surfactant can form a complex without net charge or with weak net charge. Furthermore, the stability of the o-tolyl biguanide in a whitening product system can be effectively improved, irreversible aggregation, flocculation or precipitation with common thickening agents such as carbomer is avoided, and the formula compatibility and stability of the o-tolyl biguanide are improved.

The invention discloses application of selaginella tamariscina carbon nanodots with anti-inflammatory, anti-dermatitis and skin-care functions, and relates to the field of carbon dots. The preparation method of the selaginella tamariscina extract carbon nanodots comprises the following steps: mixing a selaginella tamariscina extract with deionized water to obtain a selaginella tamariscina aqueous solution; putting the mixture into an autoclave with a polytetrafluoroethylene lining, keeping for 5-7 hours at the temperature of 160-180 DEG C, cooling, centrifuging and filtering to obtain the selaginella tamariscina extract carbon nanodots. The carbon nanodots are used for preparing medicines for resisting inflammation and atopic dermatitis and preparing skin care products.

The invention provides a healthy, safe and effective new raw material for cosmetics, namely flavone piperil ester or a salt compound thereof. The flavonoid piperazine ester can well inhibit the activity of hyaluronidase and increase the content of collagen in cells, so that the flavonoid piperazine ester has the effects of relieving, moisturizing and resisting wrinkles, meanwhile, the flavonoid piperazine ester shows remarkable DPPH free radical scavenging capacity, and the flavonoid piperazine ester is a good antioxidant. The composition can be used for preparing skin external preparations and is a good cosmetic raw material.

The present invention relates to the field of microorganisms, and in particular to Lactococcus lactis, a microbial agent, and a preparation method and application thereof. The present invention provides Lactococcus lactis JWA32 (Lactococcus lactis JWA32), and its deposit number is: CCTCC No.M 20221851. The present invention provides Lactococcus lactis, a microbial agent, and a preparation method and application thereof. The present invention provides a strain of Lactococcus lactis with excellent efficacy in preparing a skin microecological preparation, which can alleviate the oxidative damage of HaCaT cells and repair the epidermal barrier.

This invention relates to a pufferfish TRPV1 inhibitory peptide, its preparation method, and its application. The amino acid sequence of the peptide is LDIF. The preparation method includes: 1. Enzymatically hydrolyzing pufferfish skin, then inactivating the enzyme, and screening for peptides with a molecular weight not greater than 1 kDa from the hydrolysate, followed by freeze-drying to obtain the pufferfish skin enzymatic hydrolysate peptide; 2. Performing mass spectrometry analysis on the pufferfish skin enzymatic hydrolysate peptide, using mass spectrometry analysis software, and selecting multiple non-repeating peptide sequences based on the criteria of confidence level -10lgP > 20 and amino acid count < 10; 3. Molecularly docking the selected peptide sequences with the TRPV1 receptor using software to select peptide sequences with strong binding affinity to the TRPV1 receptor; 4. Solid-phase synthesis of the selected peptide sequences to obtain the peptide LDIF. This invention uses pufferfish skin as raw material to obtain a peptide with good inhibitory effect on TRPV1, which can be used to prepare skin care products for soothing sensitive skin.

The present application relates to a kind of methods for preparing skin-based eutectic gel using chrome-free tanning blank and polymerizable deep eutectic solvent.The method prepares polymerizable deep eutectic solvent with betaine and acrylic acid, dissolves initiator in it to prepare dipping solution, then adds chrome-free tanning blank, stirs for 4-48 hours at 5-30 ℃, then heats chrome-free tanning blank at 40-80 ℃ for 2-12 hours to obtain skin-based eutectic gel.The skin-based eutectic gel prepared by the method inherits the original high mechanical strength of chrome-free tanning blank, and the three-dimensional network of polymer formed in situ forms network interpenetrating structure with the original three-dimensional network of collagen fibers in leather blank, further improves the mechanical strength of skin-based eutectic gel, thereby effectively solving the problems of insufficient network structure stability, poor mechanical strength and deep eutectic solvent leakage of traditional eutectic gel.The raw materials of the present application are easy to obtain, low in price, and the preparation process is simple and easy to scale up.

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The invention provides a plant extract for promoting metabolism as well as a supercritical extraction method and application thereof, and relates to the technical field of cosmetics. The plant extract is composed of a geranium wilfordii extract, a juniper berry extract and a sea buckthorn fruit extract, after geranium wilfordii leaves are subjected to enzymolysis with compound enzyme, graded supercritical extraction is conducted, and the geranium wilfordii extract is obtained; performing quick-freezing and freeze-drying on fresh juniper berries, and performing supercritical extraction to obtain juniper berry extract; performing subcritical ultrasonic extraction on the dried sea buckthorn fruits to obtain the sea buckthorn fruit extract, and mixing the three to obtain the plant extract. The three extracts show a remarkable synergistic effect in the aspects of promoting skin metabolism and resisting inflammation, and can activate cell energy metabolism, improve microcirculation and promote tissue repair and regeneration. Cell and animal experiments prove that the composition can remarkably improve proteasome activity and inhibit inflammatory response, can be applied to cosmetics and is used for preparing skin care products with the effects of promoting metabolism, improving skin quality, resisting inflammation and relieving.

The invention relates to the technical field of preparation of dendrobium officinale extracts, in particular to a process for preparing a skin care product by utilizing dendrobium officinale extracts, which comprises the following steps: pouring granular dendrobium officinale raw materials into a material channel, simultaneously introducing liquid nitrogen with the temperature of-50 to-30 DEG C into two liquid nitrogen pipes, circularly flowing, and embrittling the dendrobium officinale raw materials through low-temperature freezing; opening the plug block to release the frozen dendrobium nobile raw material, and crushing the dendrobium nobile raw material by the rotating crushing rack; a water pump is started to extract an extracting solution into the concentration tank through the extraction cylinder, and then the extracting solution is mixed through the micro-fluidic nano-emulsification reactor to form an emulsion. A vacuum sealed gradient freezing channel is constructed through a spiral liquid nitrogen pipe-material pipe staggered nested structure, so that dendrobium officinale raw materials are embrittled step by step at low temperature, low-temperature crushing is completed by combining linkage beating of a vibrating ring-crushing frame, the retention rate of polysaccharide is increased, and the quality of the dendrobium officinale raw materials is improved. And meanwhile, a servo electric cylinder drives a plug block dynamic sealing and liquid nitrogen pipe centered inserting structure, and it is guaranteed that oxygen is isolated in the whole crushing process.

The present application relates to the technical field of skin care products, and discloses a composition with the effect of soothing sensitive skin, which is composed of the following components in mass percentage: plant extract 10-12%, skin repair factor 2-3%, jasmine hydrolat 10%, humectant 6-7%, and the balance being water. The composition with the effect of soothing sensitive skin has significant soothing effect, which is close to the soothing effect of common hormone ointments on the market. The composition can significantly reduce inflammatory factors, has good anti-inflammatory effect, simultaneously repairs damaged skin, enhances the moisturizing function, and enhances the skin barrier through the moisturizing and repairing effect. The composition has the effect of resisting anaphylaxis by reducing the content of trypsin and inhibiting the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with the effect of soothing sensitive skin has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

The present invention provides a jasmine flower oil and an extraction method and application thereof, and belongs to the technical field of flower oil extraction. The present invention freeze-dries jasmine flowers, and then uses ethanol as a solvent for microwave extraction. After obtaining an extract, the ethanol is recovered by vacuum distillation to obtain a crude extract of jasmine flower oil; the crude extract is subjected to subcritical water extraction, and the extract is centrifuged and filtered to obtain jasmine flower oil. The yield of the jasmine flower oil obtained by the present invention is as high as 7.45%, the content of the effective ingredient linalool is as high as 6.8wt%, and there is little toxic solvent residue. The extraction method of the present invention has a simple process and low energy consumption, and is suitable for large-scale production and application. The jasmine essential oil of the present invention has the effect of deep moisturizing, can also resist aging and fade fine lines, make the skin smooth and delicate, and has a calming fragrance, and can be used to prepare skin care products and spices.

The application belongs to the field of 3D biological printing and particularly relates to a hydrogel with skin damage repair function. 3+ The application proposes a preparation method of Eu 2+ mediated collagen polypeptide / sodium alginate composite hydrogel, successfully applies the hydrogel to 3D printing skin wound dressing, uses the hydrogel as biological ink, prepares skin full-layer damage dressing through 3D printing technology, and finally uses Ca 2+ The coordination between the Eu 2+ and sodium alginate enhances the stability of the hydrogel. The 3D printing skin wound dressing constructed by the application has good function of promoting skin full-layer damage repair and has application potential in the fields of tissue engineering and regenerative medicine.

The invention discloses lactobacillus paracasei AUQAVATE-5, and the preservation number of the lactobacillus paracasei AUQAVATE-5 is CGMCC (China General Microbiological Culture Collection Center) No.36510. The lactobacillus paracasei The invention also discloses a method for preparing the camellia oil by using the lactobacillus paracasei AUQAVATE-5. The invention also discloses an application of the lactobacillus paracasei AUQAVATE-5 in preparation of a skin repair product. The invention also discloses an application of the lactobacillus paracasei AUQAVATE-5 in fermentation of oils to increase the content of monoglyceride. According to the invention, the camellia oil is directly fermented by using a single lactobacillus strain so as to increase the content of monoglyceride and enhance the repairing function, the process is simple, the strain is safe, and the method is easy to control and realize large-scale production; monoglyceride is increased to an effective functional concentration, and active metabolites such as lactic acid and the like are synergistically enriched; food-grade raw materials and safe strains are used in the whole process, and the product is mild.

The application belongs to the technical field of bioengineering, and relates to a marine-derived antibacterial peptide FP2606 and a use of the marine-derived antibacterial peptide FP2606 in preparation of a skin antibacterial drug. Streptomyces globisporus The application also provides a DNA molecule encoding the antibacterial peptide, a recombinant expression vector and an engineering strain, and realizes high-efficiency heterologous expression of the FP2606 in vitro. Experimental verification shows that the antibacterial peptide FP2606 has strong bacteriostatic activity on common pathogenic bacteria causing skin infection of children, has extremely high biological safety, has no hemolytic activity, no skin cell toxicity and no skin irritation, perfectly adapts to the physiological characteristics of the tender skin of children, and can be widely applied to preparation of a skin external antibacterial preparation for children, a skin infection prevention and treatment drug for children and a skin barrier care product for children.

The present invention discloses a shell nacre-derived anti-inflammatory active peptide and its application in the preparation of skin repair products. The active peptide GEIRYEYF provided by the present invention has a concentration-dependent inhibition rate on NO release, and at a concentration of 200 μg / mL, NO release decreases by 54.32 ± 2.13%; it can also significantly reduce the secretion of pro-inflammatory cytokines TNF‑α and IL‑6 of RAW264.7 macrophages, and increase the secretion of anti-inflammatory cytokine IL‑10, and it is dose-dependent, with the highest concentration affecting IL‑6 secretion by 47.76 ± 1.02%, TNF‑α secretion by 42.01 ± 4.64%, and IL‑10 secretion by 41.71 ± 1.67%, with good anti-inflammatory activity. In addition, the active peptide has a significant proliferative effect on L929 cells, indicating that it has the potential to promote skin wound healing.

The invention discloses a method for increasing the content of pholidota chinensis fermentation product flavone and application of the pholidota chinensis fermentation product flavone in cosmetics. The method comprises the following steps: with dried pholidota chinensis pseudobulb as a raw material, crushing and sterilizing to prepare a solid-state fermentation material, inoculating lactobacillus plantarum KSD-01 for solid-state fermentation, performing ultrasonic extraction on a product obtained by fermentation by using a solvent, and performing centrifugation, vacuum concentration and freeze drying to obtain the pholidota chinensis fermentation extract. Compared with an unfermented alcohol extraction process, the method has the advantages that the total flavone extraction yield is increased from 1.61% to 3.57%, and the increase amplitude exceeds 120%; the obtained extract has remarkable free radical scavenging activity, and can be widely used for preparing skin care products with anti-oxidation, anti-inflammatory, firming and anti-wrinkle effects.

The present application provides a composition of panax notoginseng saponins and polygonum multiflorum polysaccharide compounded in a specific ratio, which has synergistic anti-inflammatory effect, better anti-inflammatory activity than the two components used alone, and can significantly inhibit the release of inflammatory factors by macrophages in inflammatory response. The composition involved in the present application can be used as a cosmetic raw material for preparing skin care products with soothing, anti-inflammatory or repairing effects.

The invention discloses a ceramide transdermal enhanced transporter preparation as well as a method and application thereof, and belongs to the technical field of transdermal delivery preparations. The preparation comprises the following components in parts by mass: 5-15 parts of hydrogenated lecithin, 1-5 parts of ceramide, 2-8 parts of cholesterol, 1-3 parts of stearic acid, 0.5-5 parts of an edge activator, 20-50 parts of absolute ethyl alcohol and 100-200 parts of ultrapure water, and the edge activator is selected from one of sodium cholate, SP60 or sucrose stearate and has a specific concentration gradient. The preparation method comprises the steps of organic phase preparation, water phase pretreatment, mixed hydration and particle size adjustment. The edge activator is used for regulating and controlling a lipid bilayer structure, the deformation index of the preparation is remarkably increased, the problems that ceramide is poor in water solubility, prone to crystallization and low in transdermal efficiency are solved, the preparation is excellent in stability, the intradermal retention amount and the skin permeation amount are remarkably higher than those of traditional lipidosome, deep skin delivery of ceramide can be achieved, and the application prospect is wide. And the composition has no skin irritation and is suitable for preparing skin barrier repairing, moisturizing or anti-inflammatory external products.

The invention relates to a polycarbonate composition as well as a preparation method and application thereof. The polycarbonate composition comprises the following components in parts by weight: 55-75 parts of polycarbonate, 14-36 parts of ultra-high molecular weight polyethylene, 2-9 parts of diamond, 1-9 parts of a phosphate compound containing a benzene ring and 0.5-5 parts of a compatilizer. The polycarbonate composition has the advantages of low bending modulus, low hardness, low friction coefficient, high impact strength and good skin touch feeling, and is very suitable for preparing skin-friendly materials.

The present application relates to the field of daily chemical products, and specifically discloses a supermolecule of ergothioneine, which is a co-crystal of ergothioneine and carnosine, wherein the molar ratio of ergothioneine to carnosine is 3:1 to 1:2. The supermolecule of the present application not only solves the problems of instability and easy degradation of ergothioneine, but also improves the skin permeability of ergothioneine, and synergistically enhances the antioxidant effect and has the anti-glycosylation effect. Therefore, it has the effects of anti-glycosylation, skin lightening, whitening, antioxidant, anti-UV, anti-early aging or anti-aging, etc., and can be used for preparing skin external agents, foods, food raw materials, daily chemical raw materials or pharmaceutical raw materials. In addition, the preparation method of the supermolecule of the present application is simple, has the advantages of high yield, low cost and suitability for large-scale production.

This invention provides a traditional Chinese medicine (TCM) whitening and freckle-removing composition and its application in the preparation of skincare products. The TCM whitening and freckle-removing composition comprises the following raw material components by weight percentage: Dendrobium nobile extract (principal ingredient) 0.1-5.0%; Astragalus membranaceus extract (assistant ingredient) 0.5-8.0%; Papaya extract 0.1-3.0%; Polygonum multiflorum extract 0.1-3.0%; Ginseng extract 0.05-2.0%; Ligusticum chuanxiong extract 0.05-2.0%; oil-soluble licorice extract (Glycyrrhiza glabra, containing glycyrrhizin) 0.01-1.0%; Carthamus tinctorius (Saffron) extract 0.01-0.5%; and Angelica sinensis extract 0.05-1.0%. Based on TCM principles, this invention uses authentic Chinese medicinal materials and modern extraction and formulation techniques to create various formulations of skincare products that offer both external whitening effects and gentle repair, thus solving the technical problem of long-term, safe, and effective whitening and freckle-removing treatment.