70 results about "Preparing skin" patented technology

The invention discloses a soothing and oil-controlling composition derived from natural plants and application thereof, and belongs to the technical field of daily cosmetics. The composition is formed by compounding an artemisia annua extract, a rehmannia root extract, a calendula extract and a pomelo fruit extract, and the four plant extracts have a synergistic effect, have the effects of repairing, relieving and controlling oil, and can be used for preparing a skin care product special for oil-sensitive skin. The composition provided by the invention can effectively solve the problems of external oil and internal dry, redness and sensitivity, grease accumulation and the like of oily sensitive skin, and meets multiple skin care requirements.

The invention belongs to the technical field of cosmetics, particularly discloses moisturizing nano-liposome emulsion prepared by a microjet method and a preparation method of the moisturizing nano-liposome emulsion, and aims to solve the problems of poor stability of active ingredients, low transdermal absorption efficiency, single effect and non-uniform particle size in a liposome preparation process in the existing moisturizing cosmetics. The nano-liposome emulsion is prepared from glycosylglycerol, acetylchitosamine, tremella polysaccharide, a myrothamnus flabellifolia leaf / stem extract, an artemisia annua extract, a lactobacillus fermentation product, flat nuclear wood oil, hydrogenated lecithin and phosphatidylcholine. The emulsion has excellent moisturizing, soothing and repairing effects, can be used for preparing skin care products such as water aqua, emulsion and cream, and has the advantages of high utilization rate of active ingredients and good stability.

The application provides a copper peptide efficient soothing composition and application thereof, which comprises copper peptide and symbiotic fermentation products of thermus thermophilus and bacillus, can target stimulation of secretion of inflammatory mediators, effectively inhibits secretion of PGE2 and expression of inflammatory factors IL-6, TNF alpha and NO, and has a synergistic effect, especially on secretion of PGE2 and expression of NO, and shows a more significant soothing effect. The composition is used as an efficacy component to prepare skin care products, is particularly suitable for people with sensitive skin, can help the skin to restore a healthy state, and has a good application prospect.

The invention provides 3D printing bio-ink as well as a preparation method and application thereof. The 3D printing bio-ink is prepared from the following components: methacrylic anhydride gelatin, oxidized hyaluronic acid, carboxymethyl chitosan and 2-methacryloyloxyethyl phosphorylcholine. By designing the specific composition of the 3D printing bio-ink, the 3D printing bio-ink which is good in printing performance, mechanical strength and electrical conductivity and high in cell survival rate after printing is prepared, and then a 3D printing stent with high cell-stent interaction is prepared and can be used for preparing a skin injury repair material.

The invention discloses application of o-tolyl biguanide in preparation of a skin whitening product, the skin whitening product and a preparation method, and belongs to the technical field of skin care. It is found for the first time that o-tolyl biguanide can effectively inhibit generation of advanced glycosylation end products, namely carboxy methyl lysine (CML), lipofuscin and melanin when serving as a whitening active ingredient, and therefore the multi-channel whitening effect is achieved. The invention further provides a whitening product, the whitening product comprises the o-tolyl biguanide and the anionic surfactant which are similar in charge quantity, the o-tolyl biguanide is positively charged, the anionic surfactant is negatively charged, and the o-tolyl biguanide and the anionic surfactant can form a complex without net charge or with weak net charge. Furthermore, the stability of the o-tolyl biguanide in a whitening product system can be effectively improved, irreversible aggregation, flocculation or precipitation with common thickening agents such as carbomer is avoided, and the formula compatibility and stability of the o-tolyl biguanide are improved.

The invention discloses application of selaginella tamariscina carbon nanodots with anti-inflammatory, anti-dermatitis and skin-care functions, and relates to the field of carbon dots. The preparation method of the selaginella tamariscina extract carbon nanodots comprises the following steps: mixing a selaginella tamariscina extract with deionized water to obtain a selaginella tamariscina aqueous solution; putting the mixture into an autoclave with a polytetrafluoroethylene lining, keeping for 5-7 hours at the temperature of 160-180 DEG C, cooling, centrifuging and filtering to obtain the selaginella tamariscina extract carbon nanodots. The carbon nanodots are used for preparing medicines for resisting inflammation and atopic dermatitis and preparing skin care products.

This invention relates to a pufferfish TRPV1 inhibitory peptide, its preparation method, and its application. The amino acid sequence of the peptide is LDIF. The preparation method includes: 1. Enzymatically hydrolyzing pufferfish skin, then inactivating the enzyme, and screening for peptides with a molecular weight not greater than 1 kDa from the hydrolysate, followed by freeze-drying to obtain the pufferfish skin enzymatic hydrolysate peptide; 2. Performing mass spectrometry analysis on the pufferfish skin enzymatic hydrolysate peptide, using mass spectrometry analysis software, and selecting multiple non-repeating peptide sequences based on the criteria of confidence level -10lgP > 20 and amino acid count < 10; 3. Molecularly docking the selected peptide sequences with the TRPV1 receptor using software to select peptide sequences with strong binding affinity to the TRPV1 receptor; 4. Solid-phase synthesis of the selected peptide sequences to obtain the peptide LDIF. This invention uses pufferfish skin as raw material to obtain a peptide with good inhibitory effect on TRPV1, which can be used to prepare skin care products for soothing sensitive skin.

The present application relates to a kind of methods for preparing skin-based eutectic gel using chrome-free tanning blank and polymerizable deep eutectic solvent.The method prepares polymerizable deep eutectic solvent with betaine and acrylic acid, dissolves initiator in it to prepare dipping solution, then adds chrome-free tanning blank, stirs for 4-48 hours at 5-30 ℃, then heats chrome-free tanning blank at 40-80 ℃ for 2-12 hours to obtain skin-based eutectic gel.The skin-based eutectic gel prepared by the method inherits the original high mechanical strength of chrome-free tanning blank, and the three-dimensional network of polymer formed in situ forms network interpenetrating structure with the original three-dimensional network of collagen fibers in leather blank, further improves the mechanical strength of skin-based eutectic gel, thereby effectively solving the problems of insufficient network structure stability, poor mechanical strength and deep eutectic solvent leakage of traditional eutectic gel.The raw materials of the present application are easy to obtain, low in price, and the preparation process is simple and easy to scale up.

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The invention provides a plant extract for promoting metabolism as well as a supercritical extraction method and application thereof, and relates to the technical field of cosmetics. The plant extract is composed of a geranium wilfordii extract, a juniper berry extract and a sea buckthorn fruit extract, after geranium wilfordii leaves are subjected to enzymolysis with compound enzyme, graded supercritical extraction is conducted, and the geranium wilfordii extract is obtained; performing quick-freezing and freeze-drying on fresh juniper berries, and performing supercritical extraction to obtain juniper berry extract; performing subcritical ultrasonic extraction on the dried sea buckthorn fruits to obtain the sea buckthorn fruit extract, and mixing the three to obtain the plant extract. The three extracts show a remarkable synergistic effect in the aspects of promoting skin metabolism and resisting inflammation, and can activate cell energy metabolism, improve microcirculation and promote tissue repair and regeneration. Cell and animal experiments prove that the composition can remarkably improve proteasome activity and inhibit inflammatory response, can be applied to cosmetics and is used for preparing skin care products with the effects of promoting metabolism, improving skin quality, resisting inflammation and relieving.

The present application relates to the technical field of skin care products, and discloses a composition with the effect of soothing sensitive skin, which is composed of the following components in mass percentage: plant extract 10-12%, skin repair factor 2-3%, jasmine hydrolat 10%, humectant 6-7%, and the balance being water. The composition with the effect of soothing sensitive skin has significant soothing effect, which is close to the soothing effect of common hormone ointments on the market. The composition can significantly reduce inflammatory factors, has good anti-inflammatory effect, simultaneously repairs damaged skin, enhances the moisturizing function, and enhances the skin barrier through the moisturizing and repairing effect. The composition has the effect of resisting anaphylaxis by reducing the content of trypsin and inhibiting the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with the effect of soothing sensitive skin has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

The application belongs to the field of 3D biological printing and particularly relates to a hydrogel with skin damage repair function. 3+ The application proposes a preparation method of Eu 2+ mediated collagen polypeptide / sodium alginate composite hydrogel, successfully applies the hydrogel to 3D printing skin wound dressing, uses the hydrogel as biological ink, prepares skin full-layer damage dressing through 3D printing technology, and finally uses Ca 2+ The coordination between the Eu 2+ and sodium alginate enhances the stability of the hydrogel. The 3D printing skin wound dressing constructed by the application has good function of promoting skin full-layer damage repair and has application potential in the fields of tissue engineering and regenerative medicine.

The invention discloses lactobacillus paracasei AUQAVATE-5, and the preservation number of the lactobacillus paracasei AUQAVATE-5 is CGMCC (China General Microbiological Culture Collection Center) No.36510. The lactobacillus paracasei The invention also discloses a method for preparing the camellia oil by using the lactobacillus paracasei AUQAVATE-5. The invention also discloses an application of the lactobacillus paracasei AUQAVATE-5 in preparation of a skin repair product. The invention also discloses an application of the lactobacillus paracasei AUQAVATE-5 in fermentation of oils to increase the content of monoglyceride. According to the invention, the camellia oil is directly fermented by using a single lactobacillus strain so as to increase the content of monoglyceride and enhance the repairing function, the process is simple, the strain is safe, and the method is easy to control and realize large-scale production; monoglyceride is increased to an effective functional concentration, and active metabolites such as lactic acid and the like are synergistically enriched; food-grade raw materials and safe strains are used in the whole process, and the product is mild.

The application belongs to the technical field of bioengineering, and relates to a marine-derived antibacterial peptide FP2606 and a use of the marine-derived antibacterial peptide FP2606 in preparation of a skin antibacterial drug. Streptomyces globisporus The application also provides a DNA molecule encoding the antibacterial peptide, a recombinant expression vector and an engineering strain, and realizes high-efficiency heterologous expression of the FP2606 in vitro. Experimental verification shows that the antibacterial peptide FP2606 has strong bacteriostatic activity on common pathogenic bacteria causing skin infection of children, has extremely high biological safety, has no hemolytic activity, no skin cell toxicity and no skin irritation, perfectly adapts to the physiological characteristics of the tender skin of children, and can be widely applied to preparation of a skin external antibacterial preparation for children, a skin infection prevention and treatment drug for children and a skin barrier care product for children.

The present invention discloses a shell nacre-derived anti-inflammatory active peptide and its application in the preparation of skin repair products. The active peptide GEIRYEYF provided by the present invention has a concentration-dependent inhibition rate on NO release, and at a concentration of 200 μg / mL, NO release decreases by 54.32 ± 2.13%; it can also significantly reduce the secretion of pro-inflammatory cytokines TNF‑α and IL‑6 of RAW264.7 macrophages, and increase the secretion of anti-inflammatory cytokine IL‑10, and it is dose-dependent, with the highest concentration affecting IL‑6 secretion by 47.76 ± 1.02%, TNF‑α secretion by 42.01 ± 4.64%, and IL‑10 secretion by 41.71 ± 1.67%, with good anti-inflammatory activity. In addition, the active peptide has a significant proliferative effect on L929 cells, indicating that it has the potential to promote skin wound healing.

The invention discloses a method for increasing the content of pholidota chinensis fermentation product flavone and application of the pholidota chinensis fermentation product flavone in cosmetics. The method comprises the following steps: with dried pholidota chinensis pseudobulb as a raw material, crushing and sterilizing to prepare a solid-state fermentation material, inoculating lactobacillus plantarum KSD-01 for solid-state fermentation, performing ultrasonic extraction on a product obtained by fermentation by using a solvent, and performing centrifugation, vacuum concentration and freeze drying to obtain the pholidota chinensis fermentation extract. Compared with an unfermented alcohol extraction process, the method has the advantages that the total flavone extraction yield is increased from 1.61% to 3.57%, and the increase amplitude exceeds 120%; the obtained extract has remarkable free radical scavenging activity, and can be widely used for preparing skin care products with anti-oxidation, anti-inflammatory, firming and anti-wrinkle effects.

The present application provides a composition of panax notoginseng saponins and polygonum multiflorum polysaccharide compounded in a specific ratio, which has synergistic anti-inflammatory effect, better anti-inflammatory activity than the two components used alone, and can significantly inhibit the release of inflammatory factors by macrophages in inflammatory response. The composition involved in the present application can be used as a cosmetic raw material for preparing skin care products with soothing, anti-inflammatory or repairing effects.

The invention discloses a ceramide transdermal enhanced transporter preparation as well as a method and application thereof, and belongs to the technical field of transdermal delivery preparations. The preparation comprises the following components in parts by mass: 5-15 parts of hydrogenated lecithin, 1-5 parts of ceramide, 2-8 parts of cholesterol, 1-3 parts of stearic acid, 0.5-5 parts of an edge activator, 20-50 parts of absolute ethyl alcohol and 100-200 parts of ultrapure water, and the edge activator is selected from one of sodium cholate, SP60 or sucrose stearate and has a specific concentration gradient. The preparation method comprises the steps of organic phase preparation, water phase pretreatment, mixed hydration and particle size adjustment. The edge activator is used for regulating and controlling a lipid bilayer structure, the deformation index of the preparation is remarkably increased, the problems that ceramide is poor in water solubility, prone to crystallization and low in transdermal efficiency are solved, the preparation is excellent in stability, the intradermal retention amount and the skin permeation amount are remarkably higher than those of traditional lipidosome, deep skin delivery of ceramide can be achieved, and the application prospect is wide. And the composition has no skin irritation and is suitable for preparing skin barrier repairing, moisturizing or anti-inflammatory external products.

The invention relates to a polycarbonate composition as well as a preparation method and application thereof. The polycarbonate composition comprises the following components in parts by weight: 55-75 parts of polycarbonate, 14-36 parts of ultra-high molecular weight polyethylene, 2-9 parts of diamond, 1-9 parts of a phosphate compound containing a benzene ring and 0.5-5 parts of a compatilizer. The polycarbonate composition has the advantages of low bending modulus, low hardness, low friction coefficient, high impact strength and good skin touch feeling, and is very suitable for preparing skin-friendly materials.

The present application relates to the field of daily chemical products, and specifically discloses a supermolecule of ergothioneine, which is a co-crystal of ergothioneine and carnosine, wherein the molar ratio of ergothioneine to carnosine is 3:1 to 1:2. The supermolecule of the present application not only solves the problems of instability and easy degradation of ergothioneine, but also improves the skin permeability of ergothioneine, and synergistically enhances the antioxidant effect and has the anti-glycosylation effect. Therefore, it has the effects of anti-glycosylation, skin lightening, whitening, antioxidant, anti-UV, anti-early aging or anti-aging, etc., and can be used for preparing skin external agents, foods, food raw materials, daily chemical raw materials or pharmaceutical raw materials. In addition, the preparation method of the supermolecule of the present application is simple, has the advantages of high yield, low cost and suitability for large-scale production.

This invention provides a traditional Chinese medicine (TCM) whitening and freckle-removing composition and its application in the preparation of skincare products. The TCM whitening and freckle-removing composition comprises the following raw material components by weight percentage: Dendrobium nobile extract (principal ingredient) 0.1-5.0%; Astragalus membranaceus extract (assistant ingredient) 0.5-8.0%; Papaya extract 0.1-3.0%; Polygonum multiflorum extract 0.1-3.0%; Ginseng extract 0.05-2.0%; Ligusticum chuanxiong extract 0.05-2.0%; oil-soluble licorice extract (Glycyrrhiza glabra, containing glycyrrhizin) 0.01-1.0%; Carthamus tinctorius (Saffron) extract 0.01-0.5%; and Angelica sinensis extract 0.05-1.0%. Based on TCM principles, this invention uses authentic Chinese medicinal materials and modern extraction and formulation techniques to create various formulations of skincare products that offer both external whitening effects and gentle repair, thus solving the technical problem of long-term, safe, and effective whitening and freckle-removing treatment.

The invention discloses a centella asiatica and larch extract compound raw material as well as a preparation method and application thereof. The compound raw material is prepared from a centella asiatica extract (the total content of asiaticoside and madecassoside is greater than or equal to 50%) and a larch extract (the purity is greater than or equal to 98%) according to the mass ratio of (1-5): 1. The preparation method comprises the steps of raw material pretreatment, centella asiatica extract preparation (ethanol reflux extraction, vacuum concentration and spray drying) and compounding and mixing, and is simple in process and low in cost. The compound raw material has multiple effects of resisting oxidation, resisting inflammation, relieving and the like, can be used for preparing skin care products such as emulsion and face cream, is wide in application, can improve the skin state and improve the performance of the skin care products, and is wide in market prospect.

The invention discloses a tranexamic acid and peptomidol composition and a preparation. The composition is formed by compounding tranexamic acid and peptidomil, the tranexamic acid and the peptidomil have a synergistic effect, the whitening and freckle-removing effects on skin are better, a preparation containing the composition has better skin compatibility, better stability and an in-vitro release effect during use, and the composition can be used for preparing freckle-removing and whitening cosmetics or medicines.

The invention provides a method for preparing skin-brightening cubilose fermentation filtrate based on a precise fermentation technology and application of the skin-brightening cubilose fermentation filtrate. Dried cubilose and a carbon source solution are sterilized, stood, cooled and mixed into a fermentation culture medium, and then activated bacillus fungicide is inoculated into the fermentation culture medium to obtain a cubilose fermentation culture medium. According to the application, microorganisms are utilized to ferment and metabolize the cubilose, extracellular protease and the like, so that the composition of cubilose substances is changed, the content of functional components in fermentation filtrate is increased, and release of skin color brightening related functional activity is accelerated and optimized. The cubilose filtrate fermented by the specific bacillus obviously reduces the melanin content and the heme content of the skin, improves the brightness of the skin, reduces the redness and the yellowness of the skin, and has the effects of removing black, removing red, removing yellowness, extracting the skin and the like; compared with enzymolysis cubilose peptide fermentation filtrate in the prior art, the accurate cubilose fermentation filtrate is free of bitter taste and peptide taste through sensory evaluation, and a solid technical foundation is laid for developing efficient and easily-absorbed cubilose-source skin-brightening functional products.

The invention relates to the technical field of biological medicine and cosmetics, and discloses an extraction method of exosomes in a leukocyte extract and application of the exosomes in skin care products, the method comprises the following steps: S1, adding anhydrous betaine into a leukocyte clarification lysate, stirring and dissolving, and constructing a hydration protective layer; s2, sodium caprylate is added, the pH is adjusted to 5.8-6.2, and surface impure protein is replaced; s3, adding polyethylene glycol to carry out low-temperature curing precipitation; and S4, redissolving and precipitating by using a phosphate buffer solution containing L-arginine, and dialyzing and filtering by using a tangential flow filtering system to obtain the L-arginine. By utilizing the synergistic effect of zwitterionic ionization and fatty acid salt replacement, the problems that exosomes are easy to agglomerate and difficult to redissolve and coprecipitation of impure proteins is serious in a traditional precipitation method are solved; the obtained exosome extract is high in purity and good in monodispersity, can be efficiently absorbed in a transdermal mode, has good skin barrier repairing and anti-inflammatory effects, and is suitable for preparing skin care products with the skin barrier repairing and anti-inflammatory effects.

The invention relates to a method for preparing a skin moisturizer through bidirectional fermentation of schizophyllum commune, the skin moisturizer and a mask, and belongs to the technical field of skin care. When the skincare lotion is prepared, the schizophyllum commune seed solution and the lotus root powder are placed in a culture medium to be fermented, and a fermentation product is filtered to obtain the skincare lotion. Schizophyllum commune seed liquid and lotus root powder are added into the culture medium for fermentation, so that the lotus root powder can be utilized to promote growth of schizophyllum commune hyphae to obtain schizophyllum commune fermentation metabolism active products, the schizophyllum commune can be utilized to effectively extract active substances in lotus roots, and by utilizing bidirectional fermentation of the schizophyllum commune and the lotus roots, the fermentation metabolism activity of the schizophyllum commune is improved. The anti-inflammatory performance and the anti-aging performance of the schizophyllum commune fermentation product can be promoted, and the skin moisturizer which is natural in formula, almost free of biological fermentation smell, fresh and cool in skin feeling and good in anti-inflammatory and anti-aging effect can be obtained.

This invention discloses lotus-derived LEA protein and its application in improving skin's resistance to dryness damage, belonging to the field of biotechnology and its application in skin care technology. The amino acid sequence of the LEA protein is shown in SEQ ID NO:1. This invention successfully constructed a recombinant expression vector containing a nucleic acid molecule encoding the LEA protein using genetic engineering methods, and transformed it into *E. coli* for efficient expression. After purification, the target protein was obtained. This LEA protein can significantly protect human immortalized keratinocytes against dryness damage. At a concentration of 100-200 μg / mL, it can restore cell viability under dryness stress to over 85%, with effects comparable to hyaluronic acid. This LEA protein has good biocompatibility and significant anti-dryness activity, and can be used to prepare skin care products that protect skin cells against dryness damage, moisturize, repair the skin barrier, or have anti-aging effects, showing broad application prospects.

The present invention belongs to the technical field of chitosan oligosaccharide modification, specifically relating to an amphiphilic chitosan oligosaccharide, its preparation method, and application. The amphiphilic chitosan oligosaccharide has the structural formula shown in Formula I: #imgabs0#, wherein m and n are both positive integers, and m+n is between 3 and 30; and -N=R represents a Schiff base structure formed by the reaction of an amino group with an aldehyde, wherein the aldehyde is citral. By grafting the aldehyde onto the chitosan oligosaccharide, the chitosan oligosaccharide possesses both amphiphilic and aldehyde properties. This amphiphilicity can be utilized to prepare skin care products in various dosage forms, while also imparting the skin care products with the fragrance and efficacy of the aldehyde.

This invention belongs to the field of cosmetic technology, specifically disclosing a moisturizing nanoliposome emulsion prepared by microfluidic method and its preparation method, aiming to solve the problems of poor stability of active ingredients, low transdermal absorption efficiency, single efficacy, and uneven particle size in the preparation process of liposomes in existing moisturizing cosmetics. This nanoliposome emulsion contains glyceryl glucoside, acetylglucosamine, Tremella fuciformis polysaccharide, Myrothamnus fruticosa leaf / stem extract, Artemisia annua extract, Lactobacillus fermentation product, Lysimachia christinae oil, hydrogenated lecithin, and phosphatidylcholine; this emulsion has excellent moisturizing, soothing, and repairing effects, and can be used to prepare skin care products such as aqueous solutions, emulsions, and creams, with the advantages of high utilization rate of active ingredients and good stability.

The invention discloses recombinant collagenase as well as a preparation method and application thereof, and belongs to the technical field of biochemical engineering. The recombinant collagenase provided by the invention is prepared from HmCol and / or CbCol, the amino acid sequence of the HmCol is as shown in SEQ ID NO: 1; the amino acid sequence of the CbCol is as shown in SEQ ID NO: 2. Recombinant collagenase hydrolytic activity experiment results show that the expressed recombinant collagenase HmCol and CbCol can recognize recombinant collagen of different sources, including fragments of human III-type collagen recombined and expressed by escherichia coli, yeast and Chinese hamster ovary cells (CHO), so that the specificity and efficient hydrolysis of the collagen are realized, and the recombinant collagenase HmCol and CbCol can be used for identifying the recombinant collagen of different sources. The material has good application prospects in the aspects of scar tissue repair, low-temperature cold compress debridement and the like, and can be used as a raw material for preparing skin repair products.