Application of selaginella tamariscina carbon nanodots with anti-inflammatory and dermatitis and skin care functions

Abstract

The invention discloses application of selaginella tamariscina carbon nanodots with anti-inflammatory, anti-dermatitis and skin-care functions, and relates to the field of carbon dots. The preparation method of the selaginella tamariscina extract carbon nanodots comprises the following steps: mixing a selaginella tamariscina extract with deionized water to obtain a selaginella tamariscina aqueous solution; putting the mixture into an autoclave with a polytetrafluoroethylene lining, keeping for 5-7 hours at the temperature of 160-180 DEG C, cooling, centrifuging and filtering to obtain the selaginella tamariscina extract carbon nanodots. The carbon nanodots are used for preparing medicines for resisting inflammation and atopic dermatitis and preparing skin care products.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of carbon dots, and particularly relates to application of a selaginella carbon nanodot with anti-inflammatory, dermatitis and skin care functions. BACKGROUND

[0002] Skin has the function of preventing water loss in the body and preventing the invasion of harmful substances. Nowadays, more and more people pursue smooth, tight and elastic skin. However, skin exposed to air for a long time is prone to lose water and also cause inflammation due to contact with some irritating substances. However, most of the commercially available skin care products contain chemical additives, which can also cause certain damage to the skin and even cause some skin problems.

[0003] Skin inflammation is a relatively common clinical symptom, and its causes are complex and varied, which may be related to contact with allergens, genetics, immunity and environmental factors. And skin diseases caused by contact are becoming more and more common, so it is more important to resist skin inflammation and strengthen skin immunity.

[0004] Atopic dermatitis (AD) is a chronic and recurrent skin disease, mainly characterized by dry, red, itchy and inflamed skin. The affected area may form weeping eczema or thickened skin, and the skin surface may have cracks. The pathogenesis of AD is complex, involving genetic factors, allergic reactions of the immune system, defects in skin barrier function and environmental factors. Among them, the abnormal reaction of the immune system to external allergens and the defect of the skin barrier function are the key mechanisms. The insufficient skin barrier function makes the skin vulnerable to allergens, bacteria or other external stimuli, and the immune system produces corresponding inflammatory response. It is manifested as an increase in serum total immunoglobulin E (IgE) levels, the release of inflammatory cytokines such as MCP-1, TNF-α, IL-1β and IL-6, and the activation of the NFκB and mitogen-activated protein kinase (MAPK, including extracellular signal-regulated kinase JNK, ERK and p38) signaling pathways. At present, the traditional treatment method of atopic dermatitis usually relies on long-term use of topical steroid ointments, ultraviolet therapy and antibiotic drugs. However, these treatment methods have many problems, such as high cost of phototherapy, significant side effects, and drug resistance caused by corticosteroid and antibiotic therapy, and the symptoms are easy to relapse and may be more severe after stopping the drug. Therefore, it is urgent to seek a safer and more effective AD treatment method, and it is necessary to replace traditional drugs with natural compounds and provide substantial relief for AD symptoms.

[0005] Carbon dots (CDs) are a new type of nanomaterials, which have excellent fluorescence properties, good biocompatibility, efficient cell absorption and low toxicity. And can be synthesized in a low-cost, simple operation way, these characteristics make carbon dots have wide application prospects in the field of biomedicine. Carbon dots can be prepared by a variety of raw materials, including organic small molecules, drug molecules, oligomers, natural herbs and plant extracts, etc. Among them, it has important significance to prepare carbon dots from natural medicine extracts for treatment. Traditional Chinese medicine extracts usually contain a variety of bioactive components, such as polyphenols, flavonoids, etc., which have antioxidant, anti-inflammatory, anticancer and other biological activities. However, these active ingredients are often limited by poor bioavailability, poor stability and many side effects in practical application. By converting natural medicine extracts into carbon dots, not only the water solubility of traditional Chinese medicine components can be improved, but also the bioavailability can be enhanced, and targeted drug delivery can be achieved by adjusting the surface properties to ensure that the drug can accurately reach the treatment site. Carbon dots prepared from precursors with biological activity can retain part of the structure and drug activity of the precursors. SUMMARY

[0006] The purpose of the present application is to solve the above problems, and provide an application of selaginella moellendorffii carbon dots with anti-inflammatory, dermatitis and skin care.

[0007] The application of selaginella moellendorffii carbon dots with anti-inflammatory, dermatitis and skin care, the selaginella moellendorffii carbon dots are selaginella moellendorffii extract carbon dots, the selaginella moellendorffii extract carbon dots are used for preparing anti-inflammatory and atopic dermatitis drugs, and the selaginella moellendorffii extract carbon dots are used for preparing skin care products.

[0008] Further, the mass concentration of the selaginella moellendorffii extract carbon dots is 2-5 mg / mL.

[0009] Further, the preparation method of the selaginella moellendorffii carbon dots with anti-inflammatory, dermatitis and skin care is carried out according to the following steps:

[0010] The selaginella moellendorffii extract is mixed with deionized water to obtain a selaginella moellendorffii water solution; the selaginella moellendorffii water solution is put into a polytetrafluoroethylene-lined autoclave, and is kept at a temperature of 160-180 DEG C for 5-7 hours, then cooled, centrifuged and filtered to obtain selaginella moellendorffii extract carbon dots.

[0011] Further, the preparation method of the selaginella moellendorffii extract is as follows:

[0012] The whole grass of selaginella moellendorffii is taken, washed, impurity removed, crushed and sieved to obtain selaginella moellendorffii powder; 70% ethanol is added according to the ratio of 1:5-15 to extract, and after extraction, the extract is concentrated and dried to obtain selaginella moellendorffii extract; wherein the extraction conditions are 3 times of extraction at room temperature, each time for 4 hours.

[0013] Furthermore, the mass concentration of the Selaginella extract carbon nanodots is 2-5 mg / mL.

[0014] Furthermore, 70% ethanol by volume is added for extraction at a material-liquid ratio of 1:5-10.

[0015] Furthermore, the centrifugation is achieved at a rotation speed of 10,000 rpm for 5 minutes.

[0016] Furthermore, the filtration is performed under a 0.22 μm filter membrane.

[0017] The present invention has the following beneficial effects:

[0018] Selaginella extract is a natural ingredient extracted from the Selaginella plant, primarily by drying the entire plant. Selaginella has a slightly pungent flavor and a mild nature. As a precious herbal essence from nature, it is rich in flavonoids, acetylphenols, and phenylpropanoid compounds. Selaginella extract has multiple pharmacological effects, including anti-inflammatory, antioxidant, antibacterial, and anti-tumor properties. The antioxidant effects of Selaginella extract are particularly significant, effectively scavenging free radicals in the body and reducing cellular damage caused by oxidative stress. Furthermore, Selaginella extract has a potent anti-inflammatory effect, inhibiting the occurrence and progression of inflammatory reactions and helping to alleviate inflammatory-related diseases. Furthermore, Selaginella extract exhibits antibacterial and antiviral biological activities, which can enhance the body's immunity and improve the body's resistance to disease. Therefore, the potent pharmacological activity exhibited by Selaginella extract gives it broad application prospects in the medical field.

[0019] This invention uses Selaginella extract as the sole carbon source to prepare Selaginella extract carbon nanodots (SSE-CDs) via a one-step hydrothermal method. The SSE-CDs have a particle size of 2-3 nm and exhibit excellent fluorescence properties, with optimal excitation wavelengths at 380 nm and 440 nm, and corresponding optimal emission wavelengths at 466 nm and 506 nm, respectively. Cell viability screening and apoptosis results demonstrated that SSE-CDs significantly repaired lipopolysaccharide (LPS)-induced inflammatory damage in human immortalized keratinocytes (Hacat). Furthermore, SSE-CDs effectively mitigated cellular damage caused by oxidative stress. In vivo animal studies further confirmed the therapeutic efficacy of SSE-CDs for atopic dermatitis. Using a 2,4-dinitrofluorobenzene (DNFB)-induced AD mouse model, the study found that SSE-CDs inhibited the production of inflammatory factors and significantly reduced the inflammatory response by inactivating proteins involved in the NF-κB and MAPK signaling pathways. Therefore, SSE-CDs, as a new type of nanoformulation, shows great potential in the treatment of skin inflammatory diseases and provides new ideas and methods for the clinical treatment of AD. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a schematic diagram of the appearance of the Selaginella moellendorffii extract carbon nanodots; (the left figure is under a bright field (under a daylight lamp), and the right figure is under a 365nm ultraviolet lamp);

[0021] Figure 2 is a fluorescence excitation characteristic diagram of the Selaginella moellendorffii extract carbon nanodots;

[0022] Figure 3 is an infrared absorption characteristic diagram of the Selaginella moellendorffii extract carbon nanodots;

[0023] Figure 4 is a transmission electron microscope diagram of the Selaginella moellendorffii extract carbon nanodots;

[0024] Figure 5 is an H&E and Masson staining effect diagram of the Selaginella moellendorffii extract carbon nanodots on the skin and skin tissue sections of a mouse atopic dermatitis model;

[0025] Figure 6 is an inflammation factor inhibition trend diagram of the Selaginella moellendorffii extract carbon nanodots on the skin of a mouse atopic dermatitis model;

[0026] Figure 7 is a PCR expression trend diagram of the Selaginella moellendorffii extract carbon nanodots on the skin of a mouse atopic dermatitis model. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the spirit of the present application will be described in detail below, and any person skilled in the art can make changes and modifications to the technology taught by the present application without departing from the spirit and scope of the present application after understanding the embodiments of the present application.

[0028] The schematic embodiments of the present application and the descriptions thereof are used to explain the present application, but are not as limitations of the present application.

[0029] Embodiment 1

[0030] The present embodiment is a preparation method of Selaginella moellendorffii carbon nanodots with anti-inflammatory, dermatitis and skin care functions, which comprises the following steps:

[0031] 1. First, the Selaginella moellendorffii extract and deionized water are configured into an aqueous solution according to a mass ratio of 1:250-600, and stirred at about 30 degrees Celsius to completely dissolve the extract.

[0032] 2. The dissolved Selaginella moellendorffii extract aqueous solution is transferred to a polytetrafluoroethylene-lined autoclave.

[0033] 3. Put the autoclave into the oven and heat it to 170°C for 6 hours to react.

[0034] 4. After the reaction is completed, put the reactor into cold water to cool down quickly.

[0035] 5. Take out the sample and put it into a centrifuge at a speed of 10000 rpm for 5 minutes.

[0036] 6. After centrifugation, take the supernatant and filter the obtained solution with a 0.22 μm water phase filter membrane to remove unreacted impurities.

[0037] 7. Finally, store the obtained Selaginella martensii extract carbon nanodot skin care water at 4°C.

[0038] Figure 1 is a schematic diagram of the appearance of Selaginella martensii extract carbon nanodots, as shown in Figure 1 under daylight lamp, the Selaginella martensii extract carbon nanodot aqueous solution is light yellow, and under 365 nm ultraviolet lamp irradiation, the Selaginella martensii extract carbon nanodot aqueous solution is blue-green fluorescent.

[0039] The fluorescence excitation characteristic diagram of Selaginella martensii extract carbon nanodots is shown in Figure 2 Selaginella martensii extract carbon nanodots have fluorescence excitation dependent properties. This means that this carbon nanodot will emit fluorescence under the excitation of light at a specific wavelength. Specifically, its optimal excitation wavelength is 370 nm, that is, when irradiated with a wavelength of 370 nm, the Selaginella martensii extract carbon nanodot will emit the strongest fluorescence. And under this excitation, its optimal emission wavelength is 450 nm, that is, the Selaginella martensii extract carbon nanodot emits the strongest fluorescence at a wavelength of 450 nm.

[0040] (1) Selaginella martensii extract carbon nanodots of the present embodiment were used for mouse experiments:

[0041] 30 KM mice were randomly divided into five groups: blank control group, model group, drug groups (low concentration, medium concentration, high concentration), six mice in each group. After 1 week of adaptive feeding, modeling began. DNFB was dissolved in a mixed solution of acetone and olive oil (acetone and olive oil were prepared in a ratio of 4:1 by volume) to prepare a 0.15% concentration of DNFB solution. One day before the experiment, the back hair of the mice was removed, with an area of about 1.5 cm x 1.5 cm, and 100 μL / cm 2 of 0.15% DNFB solution was applied to the shaved area on the back of the mice to sensitize the mice. On the 5th day of sensitization, 50 μL of 0.15% DNFB solution was used to stimulate the sensitized skin on the back, and then the drug administration began.

[0042] The results are shown in Figure 5As shown in the results, the main organs of the Control Group, Model Group, Low Dose Group, Middle Dose Group, and High Dose Group were evaluated by H&E, and it was found that Selaginella martensii carbon nanodots had no obvious toxic effects on various organs in vivo. In addition, the skin of mice in the Control Group, Model Group, Low Dose Group, Middle Dose Group, and High Dose Group was stained with H&E and Masson to observe the recovery of atopic dermatitis in mice between different groups. It was observed that the skin tissue of the model group had abnormal keratinization on the skin surface, with partial swelling, obvious inflammatory cell infiltration in the dermis surface layer, and expansion of the intercellular space. Some of the accumulated dermal layer hair follicles had no obvious inflammatory cell infiltration in the skin tissue of the administration group, and no obvious edema occurred. This indicates that Selaginella martensii carbon nanodots have a certain effect on atopic dermatitis.

[0043] The results are shown in Table 1. Figure 6 As shown in the results, interleukins are a class of cytokines produced by white blood cells (mainly lymphocytes and monocytes), which play an important role in the immune system. Interleukins are mainly involved in regulating communication between immune cells, as well as affecting the proliferation, differentiation, and function of immune cells. IL-6 and IL-1β are mainly produced by macrophages, T cells, etc., and they play a key role in the inflammatory response, promoting the proliferation and differentiation of immune cells, inducing the production of other cytokines, and thus exacerbating the inflammatory response. TNF-α is a powerful pro-inflammatory cytokine mainly produced by activated macrophages, which participates in the regulation of apoptosis, inflammatory response, and activation of immune cells, and is a key factor in many inflammatory and autoimmune diseases. IgE is mainly involved in the immune response to allergic reactions and parasitic infections, and its elevated levels are usually associated with allergic diseases. MCP-1 is a chemotactic factor that has a strong chemotactic effect on monocytes, attracting monocytes to the site of inflammation and promoting their transformation into macrophages, thus playing a role in the inflammatory response and tissue repair. Changes in the levels of these factors can reflect the activity and progression of the disease, and provide potential targets for treatment.

[0044] ELISA method was used to detect IL-6, IL-1β, TNF-α, MCP-1 and IgE in the serum of mice. It was observed that the serum IL-6 level of the Model group was significantly higher than that of the Control group (P<0.01), indicating that the modeling was successful; compared with the Model group, the IL-6 level of the Selaginella moellendorffii extract carbon nanodot group (Low Dose, Middle Dose and High Dose) gradually decreased with the increase of the drug concentration. Similarly, it was found that the serum IL-1β level of the Model group was significantly higher than that of the Control group (P<0.01), indicating that the modeling was successful; compared with the Model group, the IL-1β level of the Selaginella moellendorffii extract carbon nanodot group gradually decreased with the increase of the drug concentration. At the same time, the serum TNF-α level of the Model group was significantly higher than that of the Control group (P<0.01), indicating that the modeling was successful; compared with the Model group, the TNF-α level of the Selaginella moellendorffii extract carbon nanodot group gradually decreased with the increase of the drug concentration. For the MCP-1 factor, the same analysis showed that the serum MCP-1 level of the Model group was significantly higher than that of the Control group (P<0.01), indicating that the modeling was successful; compared with the Model group, the MCP-1 level of the Selaginella moellendorffii extract carbon nanodot group gradually decreased with the increase of the drug concentration. At the same time, it was observed that the serum IgE level of the Model group was significantly higher than that of the Control group (P<0.01), indicating that the modeling was successful; compared with the Model group, the IgE level of the Selaginella moellendorffii extract carbon nanodot group gradually decreased with the increase of the drug concentration.

[0045] (II) QT-PCR detection of tissues

[0046] 1. The tissue was taken out from the ultra-low temperature refrigerator at-80℃ and transferred to a sterile flat-bottom 1.5 mL EP tube. 1 mL Trizol reagent was added to each tube, and after being mixed thoroughly, the tissue was ground in a tissue grinder, and the bottom of the EP tube was kept in the ice box during the grinding process. Then 200 μL of chloroform solution was added to each tube, the tube cap was closed, and it was shaken thoroughly and mixed, and it was placed at room temperature 15-25℃ for 5 minutes.

[0047] 2. Each EP tube was placed in a low-temperature refrigerated centrifuge, and centrifuged at 4℃, 13000 rpm, 15 min.

[0048] 3. After centrifugation, the supernatant in the EP tube was aspirated and transferred to a new 1.5 mL EP tube. 500 μL of isopropanol solution was added to each tube, the tube cap was closed, and it was shaken and mixed, and it was placed at room temperature 15-25℃ for 10 minutes.

[0049] 4. Each EP tube was placed in a low-temperature refrigerated centrifuge, and centrifuged at 4℃, 13000 rpm, 15 min.

[0050] 5. Carefully remove the EP tube, remove the supernatant with a pipette, and then add a 75% ethanol solution diluted with non-enzyme super-heavy water to each tube for washing, vortex for 5 s, centrifuge at 4°C, 7500 rpm, and 10 min;

[0051] 6. Remove the supernatant with a pipette, and let the EP tube stand upside down on the operating table at room temperature for natural drying for 5-10 minutes;

[0052] 7. Reverse transcription of the prepared RNA according to the kit instructions;

[0053] 8. Amplification of the prepared single-stranded cDNA;

[0054] 9. Export the expression of each gene using the instrument's built-in software. The primer sequences are as follows.

[0055]

[0056] Figure 7 For the mRNA levels of MCP-1, COX2, FLG, iNOS, TNF-a, IL-1b, and IL-6 in mouse skin tissue: the related protein expression levels of the Model group compared to the Control group increased significantly, and the results had significant differences (P<0.01), which proved that the specific dermatitis model was successfully created; the related factor levels of the Selaginella extract carbon nanodot group (Low Dose, Middle Dose, and High Dose) compared to the Model group gradually decreased, and the differences were statistically significant (P<0.01), which proved that the treatment group was effective.

[0057] Example 2

[0058] Analysis of the anti-inflammatory activity of the Selaginella extract carbon nanodots prepared in Example 1

[0059] The Selaginella extract carbon nanodots prepared in Example 1 were subjected to application performance testing, and the experimental subjects were 30 people randomly selected with different skin conditions, including 10 people in each of the experimental group 1, the experimental group 2, and the experimental group 3, and each was used once every 3 days. The experiment was self-controlled before and after, and the Selaginella extract carbon nanodot skin care water was used to observe the effect of the skin care water on the skin conditions of different people.

[0060] The results are shown in Table 1.

[0061] Table 1

[0062]

[0063] Example 3

[0064] Activity study of the Selaginella extract carbon nanodots prepared in Example 1 on atopic dermatitis

[0065] Forty patients with facial atopic dermatitis were divided into a conventional group (20 patients) and a study group (20 patients). Among them, the conventional group included 10 females and 10 males; the disease duration was 3 months to 5 years, with an average of (2.5 ± 1.3) years; the age was 20-55 years, with an average of (30 ± 5) years. The study group included 10 females and 10 males; the disease duration was 3 months to 5 years, with an average of (2.5 ± 1.3) years; the age was 20-55 years, with an average of (30 ± 5) years.

[0066] (I) Method: The conventional group was treated with a water solution of Selaginella moellendorffii extract, and one layer was applied to the affected areas of the face in the morning and evening, 2 times / d. The study group was treated with the carbon nanodots of the Selaginella moellendorffii extract of Example 1 with a mass concentration of 2 mg / mL, and one layer was applied to the affected areas of the face in the morning and evening, 2 times / d.

[0067] (II) Observation index:

[0068] (1) First, the desquamation, itching degree, seborrhea degree, and skin rash diameter of the patients were scored before and after treatment, and 0-3 points were recorded according to the symptoms of no symptoms, mild, moderate, and severe. Cure: the score decreased by >90% after treatment; marked effect: the score decreased by 60%-90% after treatment; improvement: the score decreased by 20%-60% after treatment; no effect: the score decreased by <20% or increased after treatment. The total effective rate was the sum of the cure rate, the marked effect rate, and the improvement rate.

[0069] (2) The skin physiological conditions of the patients in the two groups before and after treatment were compared, including the sebum excretion rate, pH value, skin water content, and transepidermal water loss (TEWL). Before and after treatment, the patients were guided to use a mild oil-removing facial cleanser to clean their faces, and then they rested in a temperature-controlled room with a temperature of (22 ± 2) ℃ and a humidity of (45 ± 5) % for 30 min. The sebum excretion rate was detected using a skin oil and moisture measuring instrument, the pH value was detected using a skin pH test probe, the skin water content was detected using a water test probe, and the TEWL amount was detected using a skin moisture loss test instrument.

[0070] (3) Comparison of serum Cat S levels in patients in two groups at different time periods, including before treatment (T0), 1 week after treatment (T1), 2 weeks after treatment (T2), and 4 weeks after treatment (T3). T0, T1, T2, and T3 were collected in the morning in a quiet state. 3 mL of fasting venous blood was collected, and the serum was separated using a medical centrifuge at a speed of 2,500 r / min for 10 min. The detection method was enzyme-linked immunosorbent assay (ELISA). ④ Comparison of adverse reactions during treatment in patients in two groups, including the occurrence of burning, erythema, tingling, and itching.

[0071] (4) Statistical methods: The data analysis in this study used statistical software SPSS 23.0. Count data was represented by the number of cases (%) and χ2 test was used. Measurement data was represented by x ± s and t test was used. P<0.05 was considered statistically significant.

[0072] (Three) Results:

[0073] (1) Clinical efficacy: The total effective rate of the study group was 80% [(16 / 20)], with 16 cases cured, 9 cases markedly effective, 10 cases improved, and 4 cases ineffective. The total effective rate of the conventional group was 50% [(10 / 20)], with 10 cases cured, 6 cases markedly effective, 4 cases improved, and 10 cases ineffective. The difference between the two groups was statistically significant (χ2=8.726, P<0.05).

[0074] (2) Skin physiological conditions: Compared with before treatment, the sebum secretion rate and TEWL levels of patients in both groups decreased after treatment, and the study group was lower than the conventional group. The pH value and skin water content levels increased, and the study group was higher than the conventional group (P<0.05), as shown in Table 2.

[0075] (3) Serum Cat S levels in patients in two groups at different time periods were compared with before treatment. The serum Cat S levels of patients in both groups at T1, T2, and T3 after treatment were continuously decreased, and the study group was lower than the conventional group (P<0.05), as shown in Table 3.

[0076] Table 2 Comparison of skin physiological conditions between two groups of patients before and after treatment

[0077]

[0078] Table 3 Comparison of serum Cat S levels between two groups of patients at different time periods

[0079]

Claims

1. The use of Selaginella tamariscina carbon nanodots with anti-inflammatory, dermatitis and skin care, characterized in that The Selaginella uncinata carbon nanodots are Selaginella uncinata extract carbon nanodots, and the Selaginella uncinata extract carbon nanodots are used for preparing anti-inflammatory and atopic dermatitis drugs, and the Selaginella uncinata extract carbon nanodots are used for preparing skin care products.

2. The Selaginella tamariscina carbon quantum dots for use according to claim 1, wherein the Selaginella tamariscina carbon quantum dots have anti-inflammatory, dermatitis and skin care effects. The mass concentration of the Selaginella uncinata extract carbon nanodots is 2-5 mg / mL. 3.The Selaginella uncinata carbon nanodots with anti-inflammatory, dermatitis and skin care according to claim 1, characterized in that In The preparation method of the Selaginella uncinata carbon nanodots with anti-inflammatory, dermatitis and skin care is carried out according to the following steps: The Selaginella uncinata extract is mixed with deionized water to obtain a Selaginella uncinata water solution; the Selaginella uncinata water solution is placed in a polytetrafluoroethylene-lined autoclave, and the temperature is kept at 160-180 DEG C for 5-7 hours, and then the Selaginella uncinata extract carbon nanodots are obtained after cooling, centrifugation and filtration.

4. The Selaginella tamariscina carbon quantum dots for use according to claim 3, characterized in that The preparation method of the Selaginella uncinata extract is as follows: The whole grass of Selaginella uncinata is washed, impurities are removed, and the Selaginella uncinata powder is obtained after crushing and sieving; the Selaginella uncinata powder is added to 70% ethanol with a volume percentage of 70% according to a solid-liquid ratio of 1:5-15, and then the Selaginella uncinata extract is obtained after extraction, concentration and drying; wherein the extraction conditions are that the extraction is carried out at room temperature for 3 times, and each time for 4 hours. 5.The Selaginella uncinata carbon nanodots with anti-inflammatory, dermatitis and skin care according to claim 3 or 4, characterized in that The mass concentration of the Selaginella uncinata extract carbon nanodots is 2-5 mg / mL. 6.The Selaginella uncinata carbon nanodots with anti-inflammatory, dermatitis and skin care according to claim 4, characterized in that In The Selaginella uncinata extract is added to 70% ethanol with a volume percentage of 70% according to a solid-liquid ratio of 1:5-10.

7. The Selaginella tamariscina carbon nanodots for use according to claim 3, wherein the Selaginella tamariscina carbon nanodots are used for treating or preventing inflammation, dermatitis and skin care. The centrifugation is achieved at a rotation speed of 10,000 rpm for 5 min.

8. The Selaginella tamariscina carbon quantum dots for use according to claim 3, wherein the Selaginella tamariscina carbon quantum dots have anti-inflammatory, dermatitis and skin care effects. The filtration is carried out under a 0.22 μm filter membrane.

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