Soothing and oil-controlling composition derived from natural plants and application of soothing and oil-controlling composition

Abstract

The invention discloses a soothing and oil-controlling composition derived from natural plants and application thereof, and belongs to the technical field of daily cosmetics. The composition is formed by compounding an artemisia annua extract, a rehmannia root extract, a calendula extract and a pomelo fruit extract, and the four plant extracts have a synergistic effect, have the effects of repairing, relieving and controlling oil, and can be used for preparing a skin care product special for oil-sensitive skin. The composition provided by the invention can effectively solve the problems of external oil and internal dry, redness and sensitivity, grease accumulation and the like of oily sensitive skin, and meets multiple skin care requirements.

Description

Technical Field

[0001] This invention belongs to the technical field of daily cosmetics, specifically relating to a soothing and oil-controlling composition made from natural plant extracts, and the application of this composition in the preparation of repairing, soothing, and oil-controlling skin care products. Background Technology

[0002] Oily and sensitive skin often faces the dilemma of being oily on the outside but dry on the inside. On the one hand, excessive sebum secretion leads to oil accumulation, enlarged pores, and easy growth of Propionibacterium acnes, causing inflammation. On the other hand, the skin barrier is damaged, the ability to retain moisture decreases, and the tolerance to external stimuli is low, resulting in symptoms such as sensitivity and redness, forming a vicious cycle of "excessive sebum secretion causing inflammatory response and damaging the skin barrier".

[0003] Currently available skincare products often have limited soothing or oil-controlling effects from single plant extracts, making it difficult to address the multiple issues associated with oily and sensitive skin simultaneously. Research indicates that the synergistic effect of multiple plant extracts can achieve complementary benefits and enhance the overall skincare outcome. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention selects Artemisia annua extract, Rehmannia glutinosa root extract, Calendula officinalis extract, and pomelo fruit extract as core active ingredients, optimizes the ratio of the four plant extracts to achieve a synergistic effect, and provides a natural plant composition that combines soothing and oil-controlling effects.

[0005] The present invention specifically discloses the following technical solutions: In a first aspect, the present invention provides a natural plant-derived soothing and oil-controlling composition, wherein the composition contains the following components in parts by weight: Artemisia annua extract 0.04-0.1 parts; 0.125-0.5 parts of Rehmannia glutinosa root extract; Calendula extract 0.005-0.05 parts; Grapefruit extract 0.025-0.2 parts.

[0006] Preferably, the composition contains the following components in parts by weight: Artemisia annua extract 0.09-0.1 parts; 0.2-0.3 parts of Rehmannia glutinosa root extract; Calendula extract 0.02-0.03 parts; Grapefruit extract 0.03-0.05 parts.

[0007] More preferably, the composition contains the following components in parts by weight: Artemisia annua extract 0.1 part; 0.25 parts of Rehmannia glutinosa root extract; Calendula extract 0.025 parts; 0.04 parts grapefruit extract.

[0008] Secondly, the present invention provides the application of the composition described in the first aspect in the preparation of skin care products with repairing, soothing and oil-controlling effects.

[0009] Thirdly, the present invention provides a natural plant-derived soothing and oil-controlling moisturizing water, wherein the moisturizing water contains the composition described in the first aspect.

[0010] Preferably, the composition is added to the moisturizing water in an amount of 0.2-0.85%.

[0011] Preferably, the moisturizing water further contains at least one of the following: a humectant, a thickener, a pH adjuster, an antibacterial agent, a daily fragrance, and a solvent.

[0012] More preferably, the moisturizer is at least one selected from glycerin, allantoin, 1,2-hexanediol, butylene glycol, sodium hyaluronate, 1,3-propanediol, glyceryl polyether-26, trehalose, polyethylene glycol-8, and PEG / PPG-17 / 6 copolymer. The thickener is at least one of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, carbomer, acryloyl dimethyl taurate / VP copolymer, sclerotium gum, and hydroxyethyl cellulose; The pH adjuster is at least one of sodium hydroxide, arginine, and sodium citrate; The antibacterial agent is at least one of p-hydroxyacetophenone, phenoxyethanol, and triethanolamine; The solvent is deionized water.

[0013] Fourthly, the present invention provides a method for preparing the aforementioned moisturizing water, the method comprising the following steps: Step 1. Heat deionized water to 50-60℃, add thickener and pre-disperse evenly, then add humectant and stir continuously at 300-400r / min until the raw materials are completely dissolved and mixed evenly to obtain a mixture; Step 2. Add the pre-dispersed antibacterial agent (at 50-60℃) to the mixture, stir well, and turn on the cooling. Step 3. Cool the mixture to 30-40℃, add each component of the composition in sequence, stir evenly, then add daily fragrance and pH adjuster to adjust pH to 6.0-7.0, continue stirring and cooling to room temperature before discharging, and finally sieve and fill to obtain the moisturizing water.

[0014] In this invention: The core function of Artemisia annua extract is anti-inflammatory and soothing. The artemisinin it contains can be metabolized into dihydroartemisinin, which can reduce inflammatory response, soothe the skin, combat skin irritation, improve skin redness, sensitivity and other discomforts, and break the vicious cycle of "excess oil-inflammation-barrier damage". Rehmannia root extract has the core functions of controlling oil and removing acne, soothing and reducing inflammation. It inhibits the activation of inflammatory pathways, induces the release of inflammatory factors, causes oxidative stress, stimulates the endogenous chronic inflammation to actively resolve, improves skin condition, inhibits sebum secretion, and reduces the number, size and redness of acne on the skin surface. Calendula extract is primarily used to soothe redness, repair, and lock in moisture. It can effectively reduce skin sensitivity, decrease hemoglobin content, reduce the area of ​​erythema, enhance the moisture content of the stratum corneum, reduce transepidermal water loss, improve the "oily outside, dry inside" condition, strengthen the soothing and repairing effects of the composition, and has a targeted improvement effect on the irritation response of sensitive skin, thus enhancing the gentleness of skincare. The core function of grapefruit extract is to instantly reduce redness and provide long-lasting anti-allergy effects. It is rich in flavonoids and alkaloids and has a "neuro-inflammatory-itch-triple anti-allergy" effect, which targets the TRPV1 nerve pain receptor, inhibits NF-kV inflammatory factors, and reduces histamine-mediated itching. It can effectively relieve skin burning, redness and stinging, improve the skin's tolerance to external stimuli, and help other ingredients maintain the skin's water-oil balance, preventing the skin from becoming dry and tight after oil control.

[0015] The beneficial effects of this invention are: 1. In the composition provided by the present invention, four plant extracts, namely Artemisia annua extract, Rehmannia glutinosa root extract, Calendula officinalis extract and pomelo fruit extract, complement each other in a targeted manner. Artemisia annua and Rehmannia glutinosa root extract regulate sebum secretion, reduce inflammation and soothe the skin, and alleviate acne. Calendula officinalis and pomelo fruit extract reduce redness, soothe and relieve allergies. Together, they break the vicious cycle of oily and sensitive skin, and solve the three core problems of excessive oil, red and acne-prone skin, and sensitive and damaged skin. The effect is better than that of a single extract. 2. The composition provided by this invention can be applied to various repairing, soothing and oil-controlling skin care products such as moisturizing water, lotion, and face cream. The preparation process is gentle, simple to operate and easy to industrialize, and the formula can be flexibly adjusted to adjust the types of excipients to suit different skin care needs. Detailed Implementation

[0016] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0017] Unless otherwise specified, the test methods used in the examples and comparative examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified; and the percentages mentioned in the examples and comparative examples are mass percentages unless otherwise specified.

[0018] I. In this invention Artemisia annua extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.; Rehmannia glutinosa root extract: purchased from Guangzhou Youran Biotechnology Co., Ltd.; Calendula extract: purchased from Shanghai Huiwen Biotechnology Co., Ltd.; Grapefruit extract: purchased from Suzhou Nakang Biotechnology Co., Ltd.; All other raw materials used in the embodiments of this invention are commercially available.

[0019] II. Soothing and oil-controlling composition 1. Compositions 1-5 The soothing and oil-controlling compositions 1-5 were prepared by compounding according to the mass ratio of each raw material in Table 1. Table 1. Mass ratio of each raw material Raw material name Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Artemisia annua extract 0.04 0.09 0.1 0.1 0.1 Rehmannia glutinosa root extract 0.125 0.2 0.25 0.3 0.5 Calendula extract 0.005 0.02 0.025 0.03 0.05 Grapefruit extract 0.025 0.03 0.04 0.05 0.2 2. Composition 6-10 The soothing and oil-controlling composition 6-10 was obtained by compounding the raw materials according to the mass ratio of each raw material in Table 2. Table 2. Mass ratio of each raw material Raw material name Composition 6 Composition 7 Composition 8 Composition 9 Composition 10 Artemisia annua extract / 0.1 0.1 0.1 1 Rehmannia glutinosa root extract 0.25 / 0.25 0.25 1 Calendula extract 0.025 0.025 / 0.025 1 Grapefruit extract 0.04 0.04 0.04 / 1 III. Performance Testing 1. Preparation of experimental materials (1) Reagents and Samples Reagents: Dipotassium glycyrrhizate (purity ≥98%), hyaluronidase, sodium hyaluronate, thiazolyl blue (MTT, purity ≥99%), fetal bovine serum (FBS), DMEM medium (high glucose type, containing L-glutamine), human immortalized sebaceous gland cells (SZ95, passages 3-5), Nile red staining solution (concentration 100μg / mL), testosterone (purity ≥98%, 6μg / mL), linoleic acid (purity ≥99%, 100μmol / L).

[0020] 2. Soothing efficacy test (1) Experimental principle Hyaluronidase is a key enzyme in type I hypersensitivity reactions. It can specifically degrade hyaluronic acid in the skin, damage the skin barrier, and trigger inflammation. The soothing ability can be evaluated by measuring the inhibition rate of hyaluronidase activity in a sample. The higher the inhibition rate, the stronger the soothing effect.

[0021] (2) Sample preparation Dissolve each of the compositions 1-10 in sterile deionized water to prepare test solutions 1-10 with a mass fraction of 0.5%.

[0022] (3) Experimental grouping Set up 4 groups, with 4 repeating holes in each group: Blank control group: only Tris-acetic acid buffer solution, calcium chloride solution, sodium hyaluronate, distilled water, sodium hydroxide solution, acetylacetone solution, and P-DAB colorimetric reagent were added; hyaluronidase and sample were not included. Model control group: Hyaluronidase was added, no sample was included, and other reagents were the same as those in the blank control group; Positive control group: 0.02 g / mL dipotassium glycyrrhizate solution (50 μL / well) and hyaluronidase were added, and the remaining reagents were the same as those in the model control group; Sample group: Add 0.5% test solution (50μL / well) and hyaluronidase, and the remaining reagents are the same as those in the model control group.

[0023] (4) Detailed test procedures Reagent preparation: Prepare 0.2 mol / L Tris-acetic acid buffer solution (pH 5.6), 0.01 mol / L calcium chloride solution, 0.1% sodium hyaluronate solution, 0.5 mol / L sodium hydroxide solution, 1.5% acetylacetone solution, and 0.1% P-DAB colorimetric reagent; Sampling procedure: See Table 3 for detailed sampling steps; Table 3 (Unit: μL)

[0024] Absorbance measurement: After the sample was added, the absorbance of each well was measured at a wavelength of 530 nm using an ELISA reader, and the average absorbance of the four replicate wells in each group was recorded. Inhibition rate calculation: The hyaluronidase inhibition rate is calculated using the formula: Y = [1 - A / B] × 100%, where B is the average absorbance of the model control group and A is the average absorbance of each sample group.

[0025] 3. Oil control efficacy test (1) Cell safety concentration test (MTT) 1) Experimental Principle MTT can be reduced to blue-purple crystalline formazan by succinate dehydrogenase in the mitochondria of living cells. The amount of formazan produced is directly proportional to the number of living cells. By measuring the absorbance of formazan, cell viability can be calculated, and sample concentrations that are non-toxic to SZ95 cells can be screened for subsequent oil control experiments.

[0026] 2) Sample preparation Dissolve compositions 1-10 in culture medium to prepare test solutions with mass fractions of 0.1%, 0.05%, 0.025%, and 0.0125%, respectively.

[0027] 3) Experimental grouping Set up 3 groups, with 3 repeating holes in each group: Zeroing well: Contains only DMEM culture medium, no cells or samples. Blank control wells: DMEM medium and cell suspension were added, but no sample was included. Sample wells: Add DMEM culture medium + cell suspension + 0.1%, 0.05%, 0.025%, and 0.0125% of sample solution, respectively.

[0028] 4) Experimental Operation 1. Cell preparation: SZ95 cells were digested from the culture flask, resuspended in DMEM medium containing 10% FBS, and the cell density was adjusted to 8 × 10⁶ cells / year. 4 pcs / mL, for later use; 2. Inoculation and incubation: Add cell suspension and culture medium to 96-well plates according to the groups, and incubate in an incubator at 37℃ and 5% CO2 for 24 hours to allow the cells to adhere and grow. 3. Drug administration: After incubation for 24 hours, discard the old culture medium in each well, add fresh culture medium containing different concentrations of samples according to the groups, and continue to incubate in the incubator for 24 hours; 4. MTT staining: After incubation, discard the culture medium in each well, add 5 mg / mL MTT solution (20 μL / well) and fresh DMEM culture medium (180 μL / well) to each well, and continue incubation for 4 hours; 5. Crystallization dissolution: Discard the supernatant of each well, add dimethyl sulfoxide (150 μL / well) to each well, place on a shaker and shake at low speed for 10 min to completely dissolve the blue-purple crystals; 6. Absorbance measurement: The absorbance of each well was measured at a wavelength of 490 nm using an ELISA reader, and the average absorbance of the three replicate wells for each group was recorded; 7. Cell viability calculation: Calculate cell viability using the formula: H = (A 样品孔 -A 调零孔 ) / (A 对照组 -A 调零孔 () × 100%, where A sample well is the average absorbance of each sample group, A zeroing well is the average absorbance of the zeroing well, and A control group is the average absorbance of the blank control group; 8. Determination of safe concentration: The highest sample concentration with cell viability ≥90% was selected as the concentration to be used in subsequent lipid secretion inhibition experiments.

[0029] 5) Determination of safe concentration All samples showed cell viability ≥90% at a mass fraction of 0.025%, and 0.025% was determined as the concentration for subsequent oil control tests.

[0030] (2) Sebum secretion inhibition test (Nile red staining method) 1) Experimental Principle Nile red is a lipophilic fluorescent dye that can specifically bind to intracellular lipids and emit orange-red fluorescence under a fluorescence microscope. By measuring the fluorescence intensity, the intracellular lipid content can be quantified, thereby evaluating the inhibitory effect of the sample on lipid secretion in SZ95 cells. The lower the fluorescence intensity, the higher the lipid inhibition rate and the stronger the oil control effect.

[0031] 2) Experimental grouping Based on the results of cell safety concentration validation, 0.025% (volume fraction) was determined as the safe concentration for all samples. Three groups were set up, with three replicate wells in each group: Blank control group: SZ95 cells + DMEM medium, without testosterone-linoleic acid induction treatment, and without sample addition; Model control group: SZ95 cells + DMEM medium + 6 μg / mL testosterone + 100 μmol / L linoleic acid (to induce lipid synthesis), no sample added; Sample group: SZ95 cells + DMEM culture medium + 6 μg / mL testosterone + 100 μmol / L linoleic acid + 0.025% test solution.

[0032] 3) Detailed test procedures 1) Preparation of cell spreaders: Place sterile cell spreaders into 12-well plates, adding 2 mL of 3×10⁻⁶ cells per well. 5 SZ95 cell suspensions were incubated at 37°C and 5% CO2 for 24 hours to allow the cells to adhere to the cell wall. 2) Induction and drug administration: Discard the old culture medium in each well, add fresh DMEM culture medium to the blank control group, and add DMEM culture medium containing 6 μg / mL testosterone and 100 μmol / L linoleic acid to the other groups. At the same time, add the corresponding concentration of sample solution to each sample group and continue incubation for 24 h. 3) Cell fixation: After incubation, discard the culture medium, gently rinse the cell slides three times with PBS buffer, add 4% paraformaldehyde solution to each well, and fix at room temperature for 10 min; 4) Fluorescent staining: Discard the paraformaldehyde solution, rinse 3 times with PBS, add 100 μg / mL Nile Red staining solution (1 mL / well) to each well, and incubate at room temperature in the dark for 10 min; 5) Cleaning and slide preparation: Discard the staining solution, rinse 3 times with PBS, carefully remove the cell slide, blot the edges dry with filter paper, place the cell side down on the glass slide, add 1 drop of anti-fluorescence quenching mounting medium, and cover with a coverslip. 6) Fluorescence observation and analysis: Observe cell staining using a fluorescence microscope, analyze the average fluorescence intensity of each field of view using ImageJ software, and calculate the mean fluorescence intensity of each group; 7) Calculation of oil inhibition rate: The oil inhibition rate is calculated according to the formula: O=(I 模型组 -I 样品组 ) / I 模型组 ×100%, where I 模型组 I represents the mean fluorescence intensity of the model control group. 样品组 The mean fluorescence intensity of each sample group; 4. Test Results (1) Results of the soothing test Table 4 Group Average absorbance (530nm) Hyaluronidase inhibition rate (%) Blank control group 0.050±0.001 / Model control group 0.111±0.005 / Positive control group 0.086±0.003 22.52% Composition 1 0.069±0.002 37.84% Composition 2 0.066±0.002 40.54% Composition 3 0.059±0.001 46.85% Composition 4 0.063±0.003 43.24% Composition 5 0.076±0.002 31.53% Composition 6 0.088±0.004 20.72% Composition 7 0.093±0.002 16.22% Composition 8 0.096±0.005 13.51% Composition 9 0.093±0.004 16.22% Composition 10 0.081±0.003 27.03% (2) Oil control test results Table 5 Group mean fluorescence intensity Oil inhibition rate (%) Blank control group 6.32±0.34 / Model control group 8.70±1.39 / Composition 1 5.32±0.51 38.85% Composition 2 5.56±0.48 36.09% Composition 3 4.95±0.52 43.10% Composition 4 5.01±0.45 42.41% Composition 5 5.89±0.52 32.30% Composition 6 6.78±0.61 22.07% Composition 7 6.98±0.59 19.77% Composition 8 7.04±0.63 19.08% Composition 9 7.12±0.58 18.16% Composition 10 6.55±0.65 24.71% 5. Results Analysis As shown in Table 4, compositions 6-9 are control groups lacking any of the core ingredients, and their inhibition rates are all lower than those of the complete formula composition 3, proving that all four ingredients are indispensable. The complete formula using the four plant extracts is the key to achieving high-efficiency soothing. Composition 10 is a mixture of the four ingredients in equal proportions, and its inhibition rate is inferior to that of the complete formula with a specific ratio, indicating that ratio optimization is crucial to efficacy and cannot be achieved simply by mixing.

[0033] As shown in Table 5, compositions 6-9 exhibited a significant decrease in oil control due to the lack of a single core ingredient, demonstrating that the synergistic effect of the four components can maximize oil control efficacy.

[0034] IV. Application Example – Preparation of Moisturizing Water Step 1. Heat deionized water to 50-60℃, add xanthan gum, carbomer and acryloyl dimethyl taurine / VP copolymer and pre-disperse evenly, then add sodium hyaluronate, 1,3-propanediol, glycerol polyether-26, trehalose, polyethylene glycol-8 and PEG / PPG-17 / 6 copolymer in sequence, and stir continuously at 300-400 r / min until the raw materials are completely dissolved and mixed evenly to obtain a mixture; Step 2. Pre-disperse p-hydroxyacetophenone evenly with butanediol at 50-60℃, then add it to the mixture, stir evenly and start cooling; Step 3. Cool the mixture to 30-40℃, add each component of composition 3 in sequence, stir evenly, then add daily fragrance and pH adjuster to adjust pH to 6.0-7.0, continue stirring and cooling to room temperature before discharging, and finally sieve and fill to obtain the moisturizing water.

[0035] For specific dosage amounts, please refer to Table 6.

[0036] Table 6. Percentage of raw materials for moisturizing water Raw material name Application Example 1 Application Example 2 Application Example 3 Sodium hyaluronate 0.01 0.01 0.01 1,3-Propanediol 0.05 0.05 0.05 Glyceryl polyether-26 0.02 0.02 0.02 Trehalose 0.1 0.1 0.1 Polyethylene glycol-8 0.1 0.1 0.1 PEG / PPG-17 / 6 copolymer 0.08 0.08 0.08 Xanthan Gum 0.03 0.03 0.03 Carbomer 0.008 0.008 0.008 Acryloyldimethyltaurate / VP copolymer 0.01 0.01 0.01 p-Hydroxyacetophenone 0.05 0.05 0.05 Butylene glycol 0.2 0.2 0.2 Composition 3 0.2 0.6 0.85 Daily fragrance Appropriate amount Appropriate amount Appropriate amount pH adjuster Appropriate amount Appropriate amount Appropriate amount Deionized water Add to 100 Add to 100 Add to 100 Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A natural plant-derived soothing and oil-controlling composition, characterized in that, The composition contains the following components in parts by weight: Artemisia annua extract 0.04-0.1 parts; 0.125-0.5 parts of Rehmannia glutinosa root extract; Calendula extract 0.005-0.05 parts; Grapefruit extract 0.025-0.2 parts.

2. The composition according to claim 1, characterized in that, The composition contains the following components in parts by weight: Artemisia annua extract 0.09-0.1 parts; 0.2-0.3 parts of Rehmannia glutinosa root extract; Calendula extract 0.02-0.03 parts; Grapefruit extract 0.03-0.05 parts.

3. The composition according to claim 2, characterized in that, The composition contains the following components in parts by weight: Artemisia annua extract 0.1 part; 0.25 parts of Rehmannia glutinosa root extract; Calendula extract 0.025 parts; 0.04 parts grapefruit extract.

4. The composition according to any one of claims 1-3 is used in the preparation of skin care products with repairing, soothing and oil-controlling effects.

5. A natural plant-derived soothing and oil-controlling moisturizing water, characterized in that, The moisturizing water contains the composition according to any one of claims 1-3.

6. The moisturizing water according to claim 5, characterized in that, The composition is added to the moisturizing water at an amount of 0.2-0.85%.

7. The moisturizing water according to claim 6, characterized in that, The moisturizing water also contains at least one of the following: moisturizer, thickener, pH adjuster, antibacterial agent, daily fragrance, and solvent.

8. The moisturizing water according to claim 7, characterized in that, The moisturizer is at least one of glycerin, allantoin, 1,2-hexanediol, butylene glycol, sodium hyaluronate, 1,3-propanediol, glyceryl polyether-26, trehalose, polyethylene glycol-8, and PEG / PPG-17 / 6 copolymer. The thickener is at least one of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, carbomer, acryloyl dimethyl taurate / VP copolymer, sclerotium gum, and hydroxyethyl cellulose; The pH adjuster is at least one of sodium hydroxide, arginine, and sodium citrate; The antibacterial agent is at least one of p-hydroxyacetophenone, phenoxyethanol, and triethanolamine; The solvent is deionized water.

9. A method for preparing the moisturizing water according to claim 8, characterized in that, The preparation method includes the following steps: Step 1. Heat deionized water to 50-60℃, add thickener and pre-disperse evenly, then add humectant and stir continuously at 300-400r / min until the raw materials are completely dissolved and mixed evenly to obtain a mixture; Step 2. Add the pre-dispersed antibacterial agent (at 50-60℃) to the mixture, stir well, and turn on the cooling. Step 3. Cool the mixture to 30-40℃, add each component of the composition in sequence, stir evenly, then add daily fragrance and pH adjuster to adjust pH to 6.0-7.0, continue stirring and cooling to room temperature before discharging, and finally sieve and fill to obtain the moisturizing water.

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