12 results about "Fish embryo" patented technology

The application relates to a method for constructing a congenital pure red cell anemia zebra fish model, which comprises the following steps: performing directional mutation on a bysl gene in a wild-type zebra fish embryo to obtain F0 generation zebra fish; mating the F0 generation zebra fish with wild-type zebra fish to screen positive heterozygote F1 generation zebra fish from born zebra fish; mating the positive heterozygote F1 generation zebra fish with wild-type zebra fish to screen positive heterozygote F2 generation zebra fish from born zebra fish; and mating the positive heterozygote F2 generation zebra fish to screen positive homozygote zebra fish from born zebra fish as a congenital pure red cell anemia zebra fish model. The model can simulate the symptoms of congenital pure red cell anemia, and can be used for mechanism research, drug evaluation and new drug screening of the disease.

The present application relates to the technical field of skin care products, and discloses a composition with the effect of soothing sensitive skin, which is composed of the following components in mass percentage: plant extract 10-12%, skin repair factor 2-3%, jasmine hydrolat 10%, humectant 6-7%, and the balance being water. The composition with the effect of soothing sensitive skin has significant soothing effect, which is close to the soothing effect of common hormone ointments on the market. The composition can significantly reduce inflammatory factors, has good anti-inflammatory effect, simultaneously repairs damaged skin, enhances the moisturizing function, and enhances the skin barrier through the moisturizing and repairing effect. The composition has the effect of resisting anaphylaxis by reducing the content of trypsin and inhibiting the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with the effect of soothing sensitive skin has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

The invention discloses application of transgenic zebrafish in preparation of an animal model capable of visually monitoring mast cells. The transgenic zebrafish is prepared by the following method: respectively carrying out XhoI and BamHI double enzyme digestion on a cpa5 gene promoter sequence (SEQ ID NO: 1) and plasmids with fluorescent protein genes and Tol2 transposon recognition sites, and then connecting to obtain recombinant plasmids; and then introducing the recombinant plasmid and Tol2 transposase mRNA into a zebra fish embryo in a microinjection mode in a 1-cell period of zebra fish embryo development, and culturing at constant temperature to obtain the transgenic zebra fish. The cpa5 promoter is constructed to drive an expression framework of the fluorescent protein, so that the mast cells naturally emit fluorescence in the zebra fish body, and an effective animal model is provided for mechanism research and drug screening of mast cell related diseases.

The present application relates to the technical field of aquaculture, and in particular to a method and system for regulating the incubation period of dark-striped pufferfish embryos based on an effective accumulated temperature model, an electronic device, and a storage medium. The method comprises the following steps: obtaining a target incubation period based on the incubation environment temperature; obtaining the actual expected incubation temperature for each day based on the effective accumulated temperature model and the target incubation period; obtaining the current actual incubation water temperature; and adjusting the current actual incubation water temperature to approach the actual expected incubation temperature for the day using a fuzzy control system. The present application uses a fuzzy PID temperature regulation algorithm for fish embryo incubation based on an effective accumulated temperature model, thereby achieving intelligent regulation of the water temperature for factory-scale embryo incubation.

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

The utility model discloses a fish embryo separator for an ecological toxicology test in the field of ecological toxicology tests, which comprises a separating body and a handle, the handle is rod-shaped, one end of the handle is obliquely connected with the outer wall of the separating body, and the other end of the handle extends upwards and is a handheld end. According to the utility model, the special tool for separating the embryo of the fish for the ecological toxicology test is manufactured, so that the adverse effect of embryo damage possibly caused by using a non-special tool is avoided, the design convenient to operate and observe can also improve the separation quality, and the working efficiency of an operator is improved.

This application discloses a method and application for screening fish embryos based on 1,6-hexanediol-induced intracellular phase changes, belonging to the field of fish breeding technology. This method addresses the lack of non-destructive screening methods for early embryos in existing technologies. During the maternal zygote transition period, which includes fish embryonic development, 0.1% 1,6-hexanediol is used for stress treatment. Utilizing its ability to interfere with intracellular biomolecule condensation, it induces intracellular phase changes, thereby eliminating embryos with poor internal environmental stability and low robustness, while retaining high-quality embryos with high developmental potential. The screened embryos show a significantly reduced rate of deformities in the current generation, and superior traits are heritable to offspring. The fertilization rate and hatching rate of offspring are significantly improved, and the deformity rate is significantly reduced. This application is simple to operate, cost-effective, and suitable for large-scale seedling production, providing a new technical pathway for the breeding of superior fish breeds.

The invention discloses a low-temperature vacuum fried fish processing method and a low-temperature vacuum fried fish product. The processing method comprises the following steps: unfreezing fish frozen at a preset temperature in a low-temperature and high-humidity environment; performing rinsing treatment and pre-washing treatment on the unfrozen fish, and adding the fish into pickling liquid for pickling treatment to obtain fish embryos; putting the fish embryos into a fish washing pond to be subjected to cleaning and fishy smell removal treatment; performing freezing treatment on the fish embryos subjected to cleaning and fishy smell removal treatment, and performing sealed temporary storage on the frozen fish embryos at-18 DEG C; performing vacuum frying treatment on the fish embryos subjected to the freezing treatment; and mixing and uniformly stirring the fish embryos subjected to the vacuum frying treatment with seasonings. The pre-drying time in the vacuum frying process of the aquatic products can be shortened, the color uniformity of the vacuum fried products is improved, and the vacuum frying yield is increased.

The invention discloses a zebra fish embryo chemical substance toxicity evaluation system and method based on deep learning. The system comprises a data enhancement module, a data processing module and a data processing module, wherein the data enhancement module is used for executing staged differential enhancement; the development stage identification module is used for outputting a development stage classification result based on a YOLOv5m model; the toxicity end-point morphology recognition module is used for segmenting the multi-embryo instance based on a Mask R-CNN model and outputting a toxicity end-point morphology classification result; the toxicity judgment module is used for calculating a development delay index DDI according to a deviation between an actually measured morphological parameter of a development stage classification result and a standard development stage morphological parameter; receiving a toxicity end-point morphology classification result, and calculating an accumulated score TS; and classifying the toxicity risk levels of the chemical substances through weighted fusion of DDI and TS. Compared with the prior art, the method has the advantages that zebrafish embryonic development can be accurately identified, scientific quantitative evaluation of chemical substance toxicity is realized, and reliable support is provided for high-flux compound toxicity screening and environmental toxicity prediction.

The invention relates to the technical field of incubation equipment, in particular to an experimental zebra fish embryo incubation device which comprises a box body, a partition plate is fixedly connected in the box body, and an incubation assembly, an illumination assembly and a moving assembly are arranged in an incubation cavity. A driving assembly, an air supply assembly, a storage assembly, a water supply assembly and a constant temperature assembly are arranged in the operation cavity. The moving assembly and the hatching assembly are driven by the driving assembly to move in an 8 shape, zebrafish embryos at different positions in the hatching assembly can be evenly illuminated through the 8-shaped movement of the hatching assembly, the possibility that the embryos are damaged by light is reduced, and the survival rate of the embryos is increased.

The present disclosure relates to a plate for evaluating toxicants using fish embryos, a method of screening developmental neurotoxicants using the same, and an apparatus and a kit for evaluating developmental neurotoxicants including the plate. The plate and the screening method of the present disclosure provide a novel test method utilizing characteristics of the swim bladder formation process in fish embryos. This method offers an alternative to animal testing and enables effective toxicity evaluation with a small amount of sample.

The application provides a preparation method of a protein peptide of Phascolosoma esculenta and application of the protein peptide in cell repair and whitening. The preparation method of the protein peptide of Phascolosa esculenta comprises the following steps: cutting fresh and live Phascolosoma esculenta after cleaning, homogenizing the Phascolosoma esculenta by adding water, adding flavor protease and neutral protease, carrying out enzymolysis for 6 h under the condition of 55 DEG C, carrying out enzyme inactivation treatment by heating at 100 DEG C for 15 min, centrifuging to take supernatant, ultrafiltration, and freeze-drying, so that the protein peptide of Phascolosoma esculenta is prepared. The protein peptide of Phascolosoma esculenta can promote cell repair and inhibit melanin synthesis of zebra fish embryos.