22 results about "Fish embryo" patented technology

The invention relates to a method for evaluating toxicity of spiroxamine by using zebra fish and application of the method, and belongs to the technical field of toxicity detection. According to the method, spiroxamine culture solutions with different concentrations are prepared for culturing zebrafish embryos, embryonic development detection and juvenile fish behavior testing are carried out, embryonic development detection includes hatching rate, heart rate and body length, and juvenile fish behavior testing includes spontaneous movement testing, vortex escape testing, swimming behavior testing, reflex behavior testing and color stimulation testing. According to the method provided by the invention, the toxic effect of spiroxamine on zebra fish embryos and juvenile fishes is systematically and comprehensively evaluated, the intuition and accuracy of spiroxamine toxicity detection are improved, and the method can be applied to evaluating the toxicity of environmental samples containing spiroxamine.

The invention discloses a melanin content analysis method and system based on migration management and control, and relates to the technical field of quantitative analysis method.The method comprises the steps that a zebra fish embryo is shot along illumination parameters, and multiple images of the zebra fish embryo at different angles are collected; the corresponding three-dimensional data is determined according to the plurality of images, and the time sequence three-dimensional model is generated according to alignment of the three-dimensional data and the time point data, so that the accuracy of the time sequence three-dimensional model is ensured. Therefore, a melanin area is determined based on the identification of the time sequence three-dimensional model, and the three-dimensional volume of melanin is determined according to the area position of the melanin area and the voxel statistics of the melanin area; the migration path of the melanin area is collected, quantitative analysis of the melanin content is triggered according to the migration path of the melanin area, the migration speed of the melanin area and the three-dimensional volume of the melanin, and the accuracy of quantitative analysis of the melanin content is guaranteed.

The invention provides a hematopoietic anti-aging active component extracted from fish embryos as well as an extraction method and application of the hematopoietic anti-aging active component, and belongs to the technical field of active component extraction. The hematopoietic anti-aging active component is extracted from fish embryos which are developed from fish fertilized eggs to the earlier stage of incubation at the optimum temperature and stop development through rapid freezing, and is rich in exosomes, active protein, peptide, chondroitin sulfate, hyaluronic acid, small molecule metabolites, various active factors and the like, and all the nutritional active components are matched with one another, so that the effects of resisting aging and resisting aging are achieved. The cartilage development can be supported, the joint health is ensured, the hematopoietic effect is enhanced, and the skin aging is delayed. The orally disintegrating tablet containing the hematopoietic anti-aging active component can avoid inactivation of bioactive substances caused by high temperature, and the biological activity of the hematopoietic anti-aging active component is reserved to the maximum extent. The hematopoietic anti-aging active component and the orally disintegrating tablet are simple in preparation process and remarkable in hematopoietic anti-aging effect.

The application relates to a method for constructing a congenital pure red cell anemia zebra fish model, which comprises the following steps: performing directional mutation on a bysl gene in a wild-type zebra fish embryo to obtain F0 generation zebra fish; mating the F0 generation zebra fish with wild-type zebra fish to screen positive heterozygote F1 generation zebra fish from born zebra fish; mating the positive heterozygote F1 generation zebra fish with wild-type zebra fish to screen positive heterozygote F2 generation zebra fish from born zebra fish; and mating the positive heterozygote F2 generation zebra fish to screen positive homozygote zebra fish from born zebra fish as a congenital pure red cell anemia zebra fish model. The model can simulate the symptoms of congenital pure red cell anemia, and can be used for mechanism research, drug evaluation and new drug screening of the disease.

The present application relates to the technical field of skin care products, and discloses a composition with the effect of soothing sensitive skin, which is composed of the following components in mass percentage: plant extract 10-12%, skin repair factor 2-3%, jasmine hydrolat 10%, humectant 6-7%, and the balance being water. The composition with the effect of soothing sensitive skin has significant soothing effect, which is close to the soothing effect of common hormone ointments on the market. The composition can significantly reduce inflammatory factors, has good anti-inflammatory effect, simultaneously repairs damaged skin, enhances the moisturizing function, and enhances the skin barrier through the moisturizing and repairing effect. The composition has the effect of resisting anaphylaxis by reducing the content of trypsin and inhibiting the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with the effect of soothing sensitive skin has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

The invention discloses application of transgenic zebrafish in preparation of an animal model capable of visually monitoring mast cells. The transgenic zebrafish is prepared by the following method: respectively carrying out XhoI and BamHI double enzyme digestion on a cpa5 gene promoter sequence (SEQ ID NO: 1) and plasmids with fluorescent protein genes and Tol2 transposon recognition sites, and then connecting to obtain recombinant plasmids; and then introducing the recombinant plasmid and Tol2 transposase mRNA into a zebra fish embryo in a microinjection mode in a 1-cell period of zebra fish embryo development, and culturing at constant temperature to obtain the transgenic zebra fish. The cpa5 promoter is constructed to drive an expression framework of the fluorescent protein, so that the mast cells naturally emit fluorescence in the zebra fish body, and an effective animal model is provided for mechanism research and drug screening of mast cell related diseases.

The invention belongs to the field of animal models, and particularly relates to a zebrafish embryo model for 5-FU side effect research and a construction method and an evaluation method thereof. The construction method comprises the following steps: selecting zebrafish embryos for later use; and putting the zebrafish embryo into a culture hole, and then adding 5-FU for modeling treatment, thereby finishing the construction of the zebrafish embryo model for 5-FU side effect research. The 5-FU induced intestinal mucositis model is constructed by adopting a 3-5-day zebrafish embryo and analyzing information such as the survival rate, the travel trajectory, the intestinal morphology and ROS fluorescent living imaging of zebrafish, and the 5-FU induced intestinal mucositis model has the advantages of being simple in observation index, easy to observe, simple to operate, capable of saving operation time, convenient, rapid, high in efficiency and the like. The method has the characteristics of simple operation, high cost, cheaper mouse model, qualitative and quantitative properties and the like, and is suitable for high-throughput screening of 5-FU induced intestinal mucositis model drugs.

The present application relates to the technical field of aquaculture, and in particular to a method and system for regulating the incubation period of dark-striped pufferfish embryos based on an effective accumulated temperature model, an electronic device, and a storage medium. The method comprises the following steps: obtaining a target incubation period based on the incubation environment temperature; obtaining the actual expected incubation temperature for each day based on the effective accumulated temperature model and the target incubation period; obtaining the current actual incubation water temperature; and adjusting the current actual incubation water temperature to approach the actual expected incubation temperature for the day using a fuzzy control system. The present application uses a fuzzy PID temperature regulation algorithm for fish embryo incubation based on an effective accumulated temperature model, thereby achieving intelligent regulation of the water temperature for factory-scale embryo incubation.

The utility model relates to the technical field of cleaning equipment, and discloses an automatic cleaning system which comprises a basket body conveyor, an upper conveying platform and a lower conveying platform which are arranged on the basket body conveyor, a first conveying roller is arranged on the lower conveying platform, a second conveying roller is arranged on the upper conveying platform, the upper conveying platform is connected with a basket returning step, and a third conveying roller is arranged on the basket returning step. A basket overturning machine is arranged on one side of the basket body conveyor and used for overturning the rubber baskets conveyed by the first conveying roller, and the second conveying roller is used for conveying the rubber baskets reset after overturning to the third conveying roller. According to the automatic cleaning system, the first conveying roller, the basket overturning machine, the second conveying roller and the third conveying roller are matched with one another, so that the multiple rubber baskets are dumped in order, collision between the rubber baskets or between the rubber baskets and equipment is reduced, rubber basket fragments are prevented from being mixed into fish embryos, and the influence of the rubber basket fragments on subsequent fish embryo processing is reduced.

The invention provides a pelteobagrus fulvidraco embryonic cell line as well as a subculture method and application thereof, and belongs to the field of fish cell biology. The pelteobagrus fulvidraco cell line is a pelteobagrus fulvidraco embryo-derived cell line and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202516. The pelteobagrus fulvidraco embryonic cell line YCE is a fibrous cell, is continuously cultured for more than 100 generations, has a good growth state, can be stably passaged, can provide a large number of pelteobagrus fulvidraco embryonic cells, can be cryopreserved, and can still be stably passaged after being unfrozen. The fish embryo source cell line disclosed by the invention not only can be used for related research on an anti-infection immune mechanism of pelteobagrus fulvidraco, but also provides a new thought for an establishment method of a freshwater fish embryo cell line, and enriches basic biological data of fishes.

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

The utility model discloses a fish embryo separator for an ecological toxicology test in the field of ecological toxicology tests, which comprises a separating body and a handle, the handle is rod-shaped, one end of the handle is obliquely connected with the outer wall of the separating body, and the other end of the handle extends upwards and is a handheld end. According to the utility model, the special tool for separating the embryo of the fish for the ecological toxicology test is manufactured, so that the adverse effect of embryo damage possibly caused by using a non-special tool is avoided, the design convenient to operate and observe can also improve the separation quality, and the working efficiency of an operator is improved.

This application discloses a method and application for screening fish embryos based on 1,6-hexanediol-induced intracellular phase changes, belonging to the field of fish breeding technology. This method addresses the lack of non-destructive screening methods for early embryos in existing technologies. During the maternal zygote transition period, which includes fish embryonic development, 0.1% 1,6-hexanediol is used for stress treatment. Utilizing its ability to interfere with intracellular biomolecule condensation, it induces intracellular phase changes, thereby eliminating embryos with poor internal environmental stability and low robustness, while retaining high-quality embryos with high developmental potential. The screened embryos show a significantly reduced rate of deformities in the current generation, and superior traits are heritable to offspring. The fertilization rate and hatching rate of offspring are significantly improved, and the deformity rate is significantly reduced. This application is simple to operate, cost-effective, and suitable for large-scale seedling production, providing a new technical pathway for the breeding of superior fish breeds.

The present invention relates to a device and method for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos, and relates to the technical field of pollutant toxicity analysis. The device structure includes a machine base and an electric guide rail mechanism I; a carrier frame slidably arranged on the electric guide rail mechanism I, an active oscillation seat is arranged on the carrier frame, and a plurality of multi-well plates for placing fish embryos are arranged on the active oscillation seat. A lighting structure is arranged at a position below the carrier frame and at the position of the active oscillation seat; a liquid injection unit group and an incubation chamber, the liquid injection unit group is used to inject cyanotoxin working solutions with different high concentrations into a plurality of multi-well plates, and an observation unit group is arranged at the top of the incubation chamber; and an adjustable light-shielding unit group arranged between the lighting structure and the multi-well plates. The evaluation device provided by the present invention is convenient to use, reduces the manual labor intensity, improves the work efficiency, and is convenient for large-scale and high-efficiency evaluation and analysis of the developmental toxicity of high-concentration cyanotoxin to fish embryos.

The invention discloses a low-temperature vacuum fried fish processing method and a low-temperature vacuum fried fish product. The processing method comprises the following steps: unfreezing fish frozen at a preset temperature in a low-temperature and high-humidity environment; performing rinsing treatment and pre-washing treatment on the unfrozen fish, and adding the fish into pickling liquid for pickling treatment to obtain fish embryos; putting the fish embryos into a fish washing pond to be subjected to cleaning and fishy smell removal treatment; performing freezing treatment on the fish embryos subjected to cleaning and fishy smell removal treatment, and performing sealed temporary storage on the frozen fish embryos at-18 DEG C; performing vacuum frying treatment on the fish embryos subjected to the freezing treatment; and mixing and uniformly stirring the fish embryos subjected to the vacuum frying treatment with seasonings. The pre-drying time in the vacuum frying process of the aquatic products can be shortened, the color uniformity of the vacuum fried products is improved, and the vacuum frying yield is increased.

The present invention belongs to the technical field of aquaculture, and particularly relates to the application of zinc in preventing and treating infectious spleen and kidney necrosis virus infection. The present invention provides the application of zinc in the preparation of an agent for preventing and treating infectious spleen and kidney necrosis virus (ISKNV) infection. In vitro and in vivo antiviral experiments have confirmed that zinc plays a protective role against ISKNV infection. Zinc can significantly inhibit the expression of ISKNV-related genes in mandarin fish embryo cell line (MFF-1 cells), thereby inhibiting virus proliferation. By establishing disease models of cell and in vivo infection with ISKNV virus, the present invention proves that zinc is applicable to the prevention and treatment of ISKNV virus. As a drug for preventing or treating ISKNV virus infection, zinc can be used in the form of a water quality improver and an oral agent, and has the advantages of low dosage, safe ingredients, and good prevention and treatment effects.

The invention discloses a zebra fish embryo chemical substance toxicity evaluation system and method based on deep learning. The system comprises a data enhancement module, a data processing module and a data processing module, wherein the data enhancement module is used for executing staged differential enhancement; the development stage identification module is used for outputting a development stage classification result based on a YOLOv5m model; the toxicity end-point morphology recognition module is used for segmenting the multi-embryo instance based on a Mask R-CNN model and outputting a toxicity end-point morphology classification result; the toxicity judgment module is used for calculating a development delay index DDI according to a deviation between an actually measured morphological parameter of a development stage classification result and a standard development stage morphological parameter; receiving a toxicity end-point morphology classification result, and calculating an accumulated score TS; and classifying the toxicity risk levels of the chemical substances through weighted fusion of DDI and TS. Compared with the prior art, the method has the advantages that zebrafish embryonic development can be accurately identified, scientific quantitative evaluation of chemical substance toxicity is realized, and reliable support is provided for high-flux compound toxicity screening and environmental toxicity prediction.

The invention relates to the technical field of incubation equipment, in particular to an experimental zebra fish embryo incubation device which comprises a box body, a partition plate is fixedly connected in the box body, and an incubation assembly, an illumination assembly and a moving assembly are arranged in an incubation cavity. A driving assembly, an air supply assembly, a storage assembly, a water supply assembly and a constant temperature assembly are arranged in the operation cavity. The moving assembly and the hatching assembly are driven by the driving assembly to move in an 8 shape, zebrafish embryos at different positions in the hatching assembly can be evenly illuminated through the 8-shaped movement of the hatching assembly, the possibility that the embryos are damaged by light is reduced, and the survival rate of the embryos is increased.

Fluidic systems, devices and methods are provided for separating and sequestering particles from a fluid flow within traps in a fluidic chip is provided. The fluidic platform is particularly suited for parallel live zebrafish embryo studies providing automated zebrafish embryo trapping and flow through culture as well as whole mount zebrafish antibody staining functions. The zebrafish on a chip testing platform uses a chaotic hydrodynamic trapping process to trap and retain zebrafish embryos in a consistent body orientation (i.e., head pointed inward) without any external adjustments. The system and apparatus can also be adapted to be a multifunctional concentration gradient generator (CGG) that can be used to automatically immobilize dechorionated zebrafish embryos and generate chemical gradients for acute fish embryo toxicity (FET) tests.

The present disclosure relates to a plate for evaluating toxicants using fish embryos, a method of screening developmental neurotoxicants using the same, and an apparatus and a kit for evaluating developmental neurotoxicants including the plate. The plate and the screening method of the present disclosure provide a novel test method utilizing characteristics of the swim bladder formation process in fish embryos. This method offers an alternative to animal testing and enables effective toxicity evaluation with a small amount of sample.

The invention provides a construction method of a zebra fish developmental angiogenesis defect-cerebral hemorrhage model based on quintozene. Specifically, zebra fish embryos which normally develop 5-6 hours after fertilization (5-6 hpf) are exposed in a quintozene solution, and the zebra fish embryos are cultured and molded in an incubator. The invention also provides an application of quintozene in preparation of an angiogenesis defect and cerebral hemorrhage research reagent, and an application of a zebra fish developmental angiogenesis defect-cerebral hemorrhage model based on quintozene in high-throughput screening of therapeutic drugs. While the application field of quintozene is expanded, powerful improvement is provided for a modeling method of developmental angiogenesis defect-cerebral hemorrhage and a related drug screening method.

The application provides a preparation method of a protein peptide of Phascolosoma esculenta and application of the protein peptide in cell repair and whitening. The preparation method of the protein peptide of Phascolosa esculenta comprises the following steps: cutting fresh and live Phascolosoma esculenta after cleaning, homogenizing the Phascolosoma esculenta by adding water, adding flavor protease and neutral protease, carrying out enzymolysis for 6 h under the condition of 55 DEG C, carrying out enzyme inactivation treatment by heating at 100 DEG C for 15 min, centrifuging to take supernatant, ultrafiltration, and freeze-drying, so that the protein peptide of Phascolosoma esculenta is prepared. The protein peptide of Phascolosoma esculenta can promote cell repair and inhibit melanin synthesis of zebra fish embryos.