40 results about "Zebrafish embryo" patented technology

The invention belongs to the technical field of toxicity tests, and discloses a method for evaluating zebra fish developmental toxicity by single or combined use of fluopyram and tebuconazole. The method is simple, efficient and accurate, through a combined toxicity test and a single pesticide test, reference data is conveniently increased according to the influence of different environments and different medicaments on animals, the influence of pesticides on zebra fish development is calculated according to the death rate and the median lethal concentration of zebra fish embryos, the data is reasonably analyzed and processed, and the method is suitable for popularization and application. The problems that related evaluation standards formulated by depending on a single effect are not accurate and cannot truly reflect the change of the real environment quality are avoided, the environmental risk of effectively evaluating the pollutants in the environmental water body is improved, and an environmental threshold value safer for aquatic organisms is formulated conveniently.

The invention belongs to the technical field of experimental animal models, and particularly relates to a construction and analysis method of a zebra fish heart failure model. The invention discloses a construction method of a zebra fish heart failure model. The construction method comprises the following steps: S1, pretreating a zebra fish embryo; and S2, putting the zebra fish embryos pretreated in the step S1 into an exposure solution for exposure, thereby completing the construction of the zebra fish heart failure model. According to the construction and analysis method of the zebra fish heart failure model, accurate induction and dynamic monitoring of heart failure phenotypes are achieved through a standardized drug treatment process and a multi-mode heart function evaluation system, and an efficient platform is provided for cardiovascular drug screening and toxicity evaluation. The construction and analysis method of the zebra fish heart failure model further extends to the field of medicine and functional food heart protection efficacy evaluation, and objective grading authentication of food and medicine efficacy is achieved.

The application belongs to the technical field of animal model construction, and relates to a construction method and application of a zebrafish aging model. The construction method comprises the following steps: 1, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein A and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-I is obtained, and a knock-in strain I is obtained through screening; 2, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein B and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-II is obtained, and a knock-in strain II is obtained through screening; 3, the knock-in strain I and the knock-in strain II are mated, zebrafish with fluorescent protein A and fluorescent protein B simultaneously expressed in the heart are screened from the offspring, and a wrn gene knockout homozygote, that is, a zebrafish aging model, is obtained. The construction method is more convenient to breed, significantly reduces breeding costs and the workload of screening and breeding, and has good application value.

The invention belongs to the technical field of gene engineering, and particularly relates to a construction method of a ribosomal disease zebrafish model. The invention provides a construction method of a ribosomal disease zebrafish model, which comprises the following steps: directionally mutating a pno1 gene in a wild zebrafish embryo to obtain F0-generation zebrafish; mating the F0-generation zebrafish with wild type zebrafish, and screening out positive heterozygote F1-generation zebrafish from birth zebrafish as the ribosomal disease zebrafish model. The zebrafish mutant is constructed through the targeted ribosomal factor gene, multi-system phenotypes of hematopoietic system defects, skeletal insufficiency and digestive system dysplasia can be reproduced at the same time, the problem that in the prior art, disease phenotypes are incomplete is solved, and the zebrafish mutant can be widely applied to pathogenesis research and drug screening of ribosomal diseases and has a wide application prospect. An important platform is provided for developing a novel treatment means.

This provides a rapid method for comprehensively measuring the toxicity of sewage. [Solution] The invention includes the steps of: (S1) measuring zebrafish embryo toxicity indices using water samples in the full process of a sewage treatment plant, including traditional toxicity indices and behavioral toxicity indices, and performing data preprocessing; (S2) establishing a comprehensive sewage toxicity prediction model based on different target variables, with traditional toxicity indices as target variables and behavioral toxicity indices as feature variables; and (S3) inputting zebrafish embryo behavioral toxicity indices data from water samples for measurement as input data into the prediction model, selecting a corresponding prediction model according to the prediction results of the target variables, and obtaining the comprehensive toxicity of the sewage samples for measurement. The present invention is suitable for rapid measurement of comprehensive toxicity in large quantities of actual sewage samples because it can obtain standardized comprehensive toxicity indices for actual sewage samples while maintaining the advantages of speed, ease of operation, and low cost.

The invention discloses application of transgenic zebrafish in preparation of an animal model capable of visually monitoring mast cells. The transgenic zebrafish is prepared by the following method: respectively carrying out XhoI and BamHI double enzyme digestion on a cpa5 gene promoter sequence (SEQ ID NO: 1) and plasmids with fluorescent protein genes and Tol2 transposon recognition sites, and then connecting to obtain recombinant plasmids; and then introducing the recombinant plasmid and Tol2 transposase mRNA into a zebra fish embryo in a microinjection mode in a 1-cell period of zebra fish embryo development, and culturing at constant temperature to obtain the transgenic zebra fish. The cpa5 promoter is constructed to drive an expression framework of the fluorescent protein, so that the mast cells naturally emit fluorescence in the zebra fish body, and an effective animal model is provided for mechanism research and drug screening of mast cell related diseases.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The invention discloses a construction method and application of an adriamycin-induced zebrafish osteoporosis-like model, and the construction method comprises the following steps: by utilizing the characteristics of transparency and rapid bone development of zebrafish embryos and juvenile fishes, selecting the zebrafish embryos of 2dpf, and culturing the zebrafish embryos to 6dpf in an adriamycin aqueous solution of 20 mu m, so as to obtain the adriamycin-induced zebrafish osteoporosis-like model. Therefore, the visual zebrafish osteoporosis-like model is efficiently constructed, juvenile fish bodies are transparent, imaging is visual, bone development changes can be observed in vivo in real time, and visual evaluation of bone development injuries is achieved. The built zebrafish osteoporosis-like model clearly shows the systematic inhibition effect of chemotherapy drugs on bone formation and mineralization, has the advantages of being short in building period, easy and convenient to operate, high in repeatability and sensitive to drug reaction, can be used for rapid dose screening and intervention experiments, and has good application prospects. The method has great application potential in research of pathogenesis of CIOP, screening of anti-osteoporosis drugs and evaluation of curative effect of the anti-osteoporosis drugs.

This invention discloses a method for extracting and preparing polysaccharides from the traditional Chinese medicine *Cat Ginseng* and its applications. The process involves drying and pulverizing the raw material, defatting it with 95% ethanol, and then using ultrasound-assisted extraction. The liquid-to-solid ratio, extraction time, and ultrasonic power are optimized using response surface methodology. The extract is then centrifuged, concentrated, precipitated with alcohol, deproteinized and decolorized using macroporous resin, dialyzed, and freeze-dried to obtain crude polysaccharides. These crude polysaccharides are then purified by DEAE-52 and Sephadex G-150 column chromatography to obtain three homogeneous new polysaccharide fractions: AvPs1-1, AvPs2-1, and AvPs2-2. The polysaccharide extract, dominated by AvPs2-1 and AvPs2-2, not only exhibits protective effects on zebrafish embryonic development but also demonstrates significant anti-fibrotic activity in a liver injury model. Due to its natural non-toxicity, water solubility, and multiple functions including embryonic development protection and anti-fibrosis, this polysaccharide shows broad application prospects in functional foods, health products, and therapeutic drugs.

This invention relates to a method for synthesizing ascorbate glucoside via yeast lysate and its application, belonging to the field of cosmetic technology. The invention discloses a Bifida ferment lysate, which is a fermentation product lysate of *Bifidobacterium longum*, wherein the *Bifidobacterium longum* is at least one of *Bifidobacterium longum* HJ-005, *Bifidobacterium longum* subsp. *CGMCC* 1.2186, and *Bifidobacterium longum* subsp. *infant* CGMCC 1.15639; the nucleotide sequence of the 16S rRNA of *Bifidobacterium longum* HJ-005 is shown in SEQ ID NO: 1. This invention uses a specific fermentation product lysate of *Bifidobacterium longum* to catalyze the transglycosylation reaction of vitamin C, obtaining a reaction product containing AA2G, thereby increasing the yield of AA2G, significantly reducing the preparation cost of AA2G, and significantly inhibiting the production of melanin in zebrafish embryos, exhibiting a whitening effect. It can be used as a cosmetic raw material without purification.

The invention discloses a method for detecting biotoxicity of refinery wastewater based on dual levels of zebra fish embryos. The method comprises the following steps: S1, analyzing a death rate; s2, oxidative stress level analysis: respectively determining the ROS content and the SOD content of the zebrafish embryos by adopting an ROS kit and an SOD kit so as to determine the oxidative stress level of the zebrafish embryos; and S3, carrying out gene transcriptomics analysis. The invention provides a detection method for comprehensively and effectively determining the biotoxicity of the refinery wastewater by taking omics analysis as a technical means and combining determination of death rate of zebra fish embryos, morphological deformity occurrence condition and oxidative stress level, and a most scientific and reasonable detection result is finally obtained layer by layer progressively. The experimental process has the characteristics of short time period, intuitive and comprehensive experimental result and the like, and can truly reflect the biotoxicity of the refinery wastewater.

The application discloses a kind of based on the analysis method and system of melanin content of migration control, and the present application relates to the technical field of quantitative analysis method, and along the illumination parameter is photographed to zebra embryo, and multiple images of different angles of zebra embryo are collected;According to multiple images, corresponding stereoscopic data are determined, and time series three-dimensional model is generated according to the alignment of the stereoscopic data and time point data, to ensure the accuracy of time series three-dimensional model.Therefore, melanin area is determined based on the identification of time series three-dimensional model, and melanin three-dimensional volume is determined according to the area position of melanin area and the voxel statistics of melanin area;The migration path of melanin area is collected, and the quantitative analysis of melanin content is triggered according to the migration path of melanin area, the migration speed of melanin area and melanin three-dimensional volume, to ensure the accuracy of quantitative analysis of melanin content.

This invention belongs to the field of biomedical technology, specifically disclosing the application of mancozeb in the preparation of ERK inhibitors. Through zebrafish embryo models and HEK293T cell experiments, this invention demonstrates that mancozeb can specifically inhibit the MAPK / ERK pathway as an ERK inhibitor, and that at concentrations of 2μM to 8μM, it can induce a three-dimensional spiral notochord malformation in zebrafish embryos similar to that caused by 100μM U0126 treatment, providing a new approach for the preparation of MAPK / ERK pathway inhibitors.

The invention relates to an immunofluorescence method and kit for detecting zebra fish heart DNA methylation level, and belongs to the technical field of biological detection. The immunofluorescence method provided by the invention comprises the following steps: exposing a zebrafish embryo to a substance to be detected, dissecting a heart sample of the zebrafish embryo and carrying out fixation, membrane permeation, protease K digestion, hydrochloric acid DNA denaturation and neutralization treatment, and then carrying out sealing, incubation of a 5-methylcytosine primary antibody and a fluorescently labeled secondary antibody, cell nucleus staining and fluorescence detection. And finally, analyzing and evaluating the DNA methylation level through fluorescence imaging. According to the method disclosed by the invention, through a combined step of protease K and hydrochloric acid denaturation treatment, the binding efficiency to the antibody is remarkably improved, so that high-sensitivity and low-background detection on the methylation level of the whole DNA in a zebra fish micro heart sample is realized; the detection kit provided by the invention can be widely applied to epigenetics evaluation of toxicity of environmental pollutants.

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

The invention discloses a construction method and application of a multi-organ development injury animal model based on non-coding long-chain RNA (Ribonucleic Acid). According to the present invention, it is found that the expression level of LncRNA URS0000A7E830 is significantly increased compared to the normal control group in the embryonic period amoxicillin exposed zebra fish model, and the URS0000A7E830 siRNA microinjection intervention is performed on the fertilized one-cell period zebra fish embryo based on the embryonic period amoxicillin exposed zebra fish model so as to effectively improve the multi-organ differentiation damage. The model established by the invention is novel, reliable, simple and convenient, and has important significance for illuminating the occurrence mechanism of the development injury of multiple organs and determining clinical early warning and intervention targets.

The present invention relates to zebrafish embryo and adult models constructed by inducing inflammation with carboxymethyllysine (CML), which causes glycation toxicity, and a method for screening anti-inflammatory agents using the same. The present invention provides a method for screening a novel anti-inflammatory agent using zebrafish in which embryonic death, acute cytokine storm, and neuro-paralysis has been induced, whereby inflammatory diseases can be prevented or treated in a more convenient and cost-effective manner.

The invention discloses a zebra fish-based spatial resolution lipidomics method, and belongs to the technical field of biological medicines. The zebrafish-based spatial resolution lipidomics method provided by the invention comprises the following steps: separating a zebrafish trunk from yolk; sample pretreatment and chromatography-mass spectrometry detection. According to the method, yolk is sucked by a pipettor, trunks are cleaned in batches by a vortex instrument, after the trunks and the yolk are pretreated, a sample is subjected to lipidomics detection by an ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap high-resolution mass spectrometer, and further data analysis is performed. According to the method, the trunk and yolk samples which can be used for high-quality and high-coverage lipidomics analysis are obtained while high sample flux is maintained for the first time, and regionalized analysis of zebrafish embryo / larva lipid metabolism is realized. The method has the advantages of simple sample mechanism, low lipid signal interference, high sensitivity and the like, and can detect inrecognizable subtle changes and key lipid molecules in the whole sample.

The invention discloses a method for creating fluorescent zebra fish based on an enhancer of a muscle specific gene. The invention provides a method for creating fluorescent zebrafish, which comprises the following steps: by taking an upstream promoter and an enhancer of a fmyhc2.2 gene as target sequences, amplifying the upstream promoter and the enhancer and seamlessly connecting the upstream promoter and the enhancer with a fluorescent protein carrier to form a recombinant plasmid, carrying out microinjection on a zebrafish embryo, selecting F0 generation individuals capable of driving fluorescent protein expression, and culturing until sexual maturity, thereby obtaining the fluorescent zebrafish. Selecting male and female individuals for selfing to obtain F1 generation, observing and selecting individuals with strong fluorescence from F1 generation embryos through a fluorescence microscope, and culturing the individuals with strong fluorescence to sexual maturity to obtain the transgenic zebrafish with remarkably discolored skin. The fluorescent zebra fish is created by utilizing the enhancer of the muscle specific gene, an innovative method is provided for creating a zebra fish ornamental strain, a support is provided for researching a regulation mechanism of zebra fish muscle related genes, and the fluorescent zebra fish has important scientific value and industrial application value.

The application discloses a robot cell microinjection operation method based on dynamic balance game mechanism reinforcement learning. The method comprises the following steps: training an injection operation decision model using a total loss function; the total loss function comprises a loss of a PPO reinforcement learning network, an entropy regularization term and a state prediction loss of a state prediction network; during the robot cell microinjection operation process, injection environment images are collected in real time, input into an instance segmentation network model, and environment mask images are obtained; the injection needle tip real-time coordinates and cell mask images are obtained according to the environment mask images; a feature extraction network receives the cell mask images, a PPO reinforcement learning network receives the feature extraction results and the injection needle tip real-time coordinates, and the expected position of the injection needle tip is obtained as feedback information to control the robot. The application can perform safe and high-precision zebrafish embryo cell microinjection operation in a real scene, and provides technical support for the fields of gene editing and cell breeding.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

The invention provides a gene ucp1 related to cold resistance of fishes and application of the gene ucp1 in cold-resistant breeding of the fishes, and belongs to the technical field of gene engineering. Based on zebrafish developmental biology and molecular biology research, a gene ucp1 related to fish cold resistance is found, based on a CRISPR / Cas9 technology, a CRISPR / Cas9 gene editing target site of the ucp1 gene is constructed, a zebrafish embryo is converted, and transgenic zebrafish with ucp1 knocked out is obtained. Results show that knockout expression of the ucp1 can improve the cold resistance of the zebra fish and relieve fish fever reaction caused by viruses, which indicates that the ucp1 gene is a gene for regulating and controlling the cold resistance of the fish and has a wide application prospect in the aspect of breeding of the cold-resistant fish.

A chemical reduction detoxification method for 6PPD-quinone, an aminoquinone compound derived from tire rubber antioxidants, is disclosed. The method involves mixing wastewater containing 6PPD-Q with a sodium sulfite solution and incubating at room temperature for 30 minutes. This reduction treatment converts the quinone group to hydroquinone, thereby eliminating its ability to participate in redox cycles and generate reactive oxygen species. The detoxification rate was as high as 92.4% in zebrafish embryo mortality tests and as high as 97.3% in bacterial luminescence inhibition tests. This invention provides an efficient and economical method for mitigating the ecological risks posed by tire-derived aminoquinone pollutants in water systems.

The invention provides a construction method of a Sec24b gene knockout zebrafish model, which comprises the following steps: designing a target site according to a Sec24b gene sequence, carrying out in-vitro transcription on gRNA, mixing the gRNA and Cas9 protein, injecting the mixture into a single-cell stage zebrafish embryo to obtain an F0 generation embryo, and carrying out a series of screening identification and passage to finally obtain a stably inherited Sec24b gene deleted zebrafish. Compared with a traditional method, the technology has the advantages of being higher in knockout efficiency, not prone to off-target, smaller in embryotoxicity and the like. The zebrafish with the Sec24b gene knocked out, which is constructed by the invention, can be stably inherited and can be used for researching skeletal development diseases.

The application provides an antioxidant composition and a preparation method and application thereof, and belongs to the field of cosmetic raw materials. The application adopts mixed strains of Brevibacterium flavum ATCC21493, Corynebacterium glutamicum ATCC14067 and Bacillus subtilis ATCC6633 to ferment raw materials of tryptone, yeast extract, vitamin B1 and biotin, so that a fermentation product with rich small molecule amino acids, gamma-aminobutyric acid and high activity is obtained, and the fermentation product has good antioxidant, soothing, moisturizing and repairing effects and has a wide application prospect in cosmetics. The prepared cosmetic shows significant activity in zebrafish embryo test, has high safety, does not disturb the functions of the body itself while realizing antioxidant, and has extremely high biological safety and universality.

This application discloses a nuclear localization signal AoNLS and its application in enhancing CRISPR / Cas9 editing in zebrafish. The nuclear localization signal AoNLS is derived from the zebrafish proteome. Through bioinformatics mining and functional screening, a structurally unique amino acid, KRKREAE, was identified, with its amino acid sequence shown in SEQ ID NO:1. This sequence is composed of both basic and acidic amino acids, and its mode of action differs from the single charge-driven mechanism of traditional NLS, achieving a breakthrough improvement in nuclear input efficiency. The Pcs2-AoNLS-GFP system, constructed by fusing AoNLS with GFP protein, showed a 1.56-fold increase in nuclear input efficiency in zebrafish embryos compared to the traditional SV40 NLS. Simultaneously, the Pcs2-AoNLS-Cas9 system, constructed by fusing AoNLS with Cas9 protein, significantly enhanced the editing efficiency of the Stat3 and Amh target genes by 1.72 times and 1.82 times, respectively, compared to the Pcs2-SV40 NLS-Cas9 gene editing system. The AoNLS sequence provided in this application is short and highly efficient, and can be used as a core component of the next-generation gene editing tool nuclear delivery system, with good application prospects in the fields of aquaculture breeding and developmental biology research.

The invention discloses a zebra fish embryo chemical substance toxicity evaluation system and method based on deep learning. The system comprises a data enhancement module, a data processing module and a data processing module, wherein the data enhancement module is used for executing staged differential enhancement; the development stage identification module is used for outputting a development stage classification result based on a YOLOv5m model; the toxicity end-point morphology recognition module is used for segmenting the multi-embryo instance based on a Mask R-CNN model and outputting a toxicity end-point morphology classification result; the toxicity judgment module is used for calculating a development delay index DDI according to a deviation between an actually measured morphological parameter of a development stage classification result and a standard development stage morphological parameter; receiving a toxicity end-point morphology classification result, and calculating an accumulated score TS; and classifying the toxicity risk levels of the chemical substances through weighted fusion of DDI and TS. Compared with the prior art, the method has the advantages that zebrafish embryonic development can be accurately identified, scientific quantitative evaluation of chemical substance toxicity is realized, and reliable support is provided for high-flux compound toxicity screening and environmental toxicity prediction.

The application discloses a method for evaluating health risks of human pesticide mixed exposure by using a zebrafish embryo model, and comprises the following steps: 1) configuring pesticide exposure solutions with different concentrations; 2) culturing zebrafish embryos in the pesticide exposure solutions for 96 hours; 3) recording development indexes of the zebrafish embryos during the culturing process; 4) calculating toxicity endpoints according to the development indexes in the step 3); and 5) extrapolating the toxicity endpoints to human exposure level reference values. The application uses zebrafish as a model, measures different physiological indexes after pesticide mixed exposure, screens the most sensitive indexes by using a mathematical model, and finally provides intake reference values for human exposure to pesticide mixed pollution by using dose extrapolation calculation.

The invention relates to a method for evaluating a heavy metal cadmium (Cd) and polystyrene micro-nano plastic (PS) combined pollution ecological risk based on intestinal flora functions in a fish development period and a method for intervening and relieving drug screening. The method comprises the following steps: (1) respectively exposing zebrafish embryos to pollutants to be detected and a control solution in a period of 8-120 hpf after fertilization; (2) evaluating development indexes of zebrafish embryos in the exposure period; (3) evaluating the neural behavior of the zebrafish embryo during the exposure period; (4) determining the composition and diversity of the exposed zebrafish embryo intestinal microbiota through 16S rRNA high-throughput sequencing; and (5) detecting the biochemical index level of the exposed zebrafish embryo. The influence mechanism of Cd and PS composite exposure on zebra fish intestinal flora is disclosed from the perspective of host-microorganism interaction, a microbiological basis is provided for ecological risk assessment of composite pollutants in a water environment, and a potential target is provided for relieving pollution toxicity through antibiotic intervention.