76 results about "Zebrafish embryo" patented technology

The present invention belongs to the field of cosmetics detection, and discloses a method for evaluating the sun protection function of cosmetics and cosmetic raw materials and its application. This evaluation method uses a non-contact drug delivery device to test the sun protection effect of the test samples, and the test samples are finished cosmetics or diluted cosmetic raw materials; the tests include evaluating the in vitro sun protection efficacy by skin cells, evaluating the in vivo sun protection efficacy by the zebrafish embryo model, and result evaluation. This method evaluates the anti-aging effect of sun protection through the expression of β-galactosidase and / or the secretion of collagen in human dermal fibroblasts, and evaluates the sunburn prevention effect through the survival rate of human keratinocytes, DNA damage, or the secretion of inflammatory factors and the lethal / teratogenic conditions of zebrafish embryos, realizing multi-level and all-round evaluation of the sun protection efficacy. The method provided by the present invention is applicable to the evaluation of the sun protection function of various sun protection products, has accuracy and practicability, and provides an important technical basis for the research and development, quality control, and performance optimization of sun protection cosmetics.

The invention relates to the technical field of freeze-dried powder preparation, and discloses a white tomato exosome freeze-dried powder preparation method, which comprises: 1, manually picking white tomatoes 3-5 days after a color changing period, carrying out double-frequency ultrasonic cleaning, and pre-cooling in a 4 DEG C phosphate buffer solution containing 0.1% of vitamin C; according to the method, by virtue of an ultrasonic-cellulase synergistic crushing process, cells are mildly and efficiently crushed, so that the damage to an exosome structure is reduced, and the release efficiency and activity of the exosome are improved; the zebra fish embryo melanogenesis can be obviously inhibited, and the whitening and freckle-removing effects are relatively good.

The invention relates to a method for evaluating toxicity of spiroxamine by using zebra fish and application of the method, and belongs to the technical field of toxicity detection. According to the method, spiroxamine culture solutions with different concentrations are prepared for culturing zebrafish embryos, embryonic development detection and juvenile fish behavior testing are carried out, embryonic development detection includes hatching rate, heart rate and body length, and juvenile fish behavior testing includes spontaneous movement testing, vortex escape testing, swimming behavior testing, reflex behavior testing and color stimulation testing. According to the method provided by the invention, the toxic effect of spiroxamine on zebra fish embryos and juvenile fishes is systematically and comprehensively evaluated, the intuition and accuracy of spiroxamine toxicity detection are improved, and the method can be applied to evaluating the toxicity of environmental samples containing spiroxamine.

The invention discloses a red fluorescent silicon nanodot as well as a preparation method and application thereof. The method comprises the following steps: S1, weighing sulforhodamine 101, adding ultrapure water for dissolving, then adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, uniformly stirring, transferring the obtained mixture into a reaction kettle, and heating for reaction; and S2, after the reaction is finished, cooling to room temperature, purifying the obtained product by using a silica gel chromatographic column, eluting by using an eluent, collecting the eluent, and finally, freeze-drying to obtain the red fluorescent silicon nanodots. Compared with common blue-green fluorescence, red fluorescence emission has deeper tissue penetrability and background fluorescence interference resistance; the silicon nanodot provided by the invention not only has excellent light stability and high quantum yield, but also has characteristics similar to lipophilic cations, and can specifically target mitochondria in living cells; in addition, the silicon nanodots also show excellent imaging ability in zebrafish embryos and juvenile fish bodies.

The invention belongs to the technical field of biological medicine, and particularly relates to a construction method of a heat-syndrome zebrafish model, the model and application. The construction method comprises the following steps: adding a hot drug into a fluorescent zebrafish embryo for induction; the preparation method of the heat medicine comprises the following steps: S1, weighing traditional Chinese medicine heat syndrome whole-formula medicinal materials; s2, after water is added, heating and boiling are conducted, and suction filtration is conducted after reflux extraction; s3, repeating the step S2 twice; s4, combining the three filtrates, and freeze-drying after rotary evaporation to obtain traditional Chinese medicine heat syndrome whole-formula powder; and S5, dissolving to obtain the hot medicine. According to the construction method, the number of macrophages at the inflammation part of the zebra fish is remarkably increased, so that immune overexpression is caused, and the heat-syndrome zebra fish model is successfully obtained. The construction method disclosed by the invention is simple and convenient to operate, short in modeling time, high in experimental efficiency and easy to observe modeling indexes, and can be used for researching pathogenesis and treatment methods of the heat syndrome, screening drugs or vaccines for preventing or treating the heat syndrome and testing the safety and effectiveness of the drugs.

The invention discloses a melanin content analysis method and system based on migration management and control, and relates to the technical field of quantitative analysis method.The method comprises the steps that a zebra fish embryo is shot along illumination parameters, and multiple images of the zebra fish embryo at different angles are collected; the corresponding three-dimensional data is determined according to the plurality of images, and the time sequence three-dimensional model is generated according to alignment of the three-dimensional data and the time point data, so that the accuracy of the time sequence three-dimensional model is ensured. Therefore, a melanin area is determined based on the identification of the time sequence three-dimensional model, and the three-dimensional volume of melanin is determined according to the area position of the melanin area and the voxel statistics of the melanin area; the migration path of the melanin area is collected, quantitative analysis of the melanin content is triggered according to the migration path of the melanin area, the migration speed of the melanin area and the three-dimensional volume of the melanin, and the accuracy of quantitative analysis of the melanin content is guaranteed.

The invention discloses a medicine for treating or relieving heart failure, the medicine comprises an estrogen compound, and the estrogen compound is preferably mestraanol. According to the invention, verapamil hydrochloride is used for inducing and establishing the chronic heart failure model of zebrafish, and direct exposure of the estrogen compound MESTRANOL is carried out on a zebrafish embryo with heart failure, so that the heart failure of zebrafish is relieved. The phenotype of zebrafish is observed and analyzed, and physiological data, gene expression quantity related to heart failure and key events causing heart failure in rat H9c2 myocardial cells, such as ROS level and cell apoptosis, are counted; the estrogen compound MESTRANOL is innovatively found and proved to be capable of relieving heart rate decline, venous sinus extravasated blood, pericardial cavity enlargement and heart cyclization abnormity caused by heart failure, and has more excellent drug effects compared with positive drugs E2 and G1, so that a foundation is laid for developing new drugs for treating heart failure.

The invention belongs to the field of daily chemicals, and discloses a composition with light damage resistance, which comprises a ribonucleic acid solution, tetrahydromethylpyrimidine carboxylic acid and a spirulina maxima extract solution in a weight ratio of (60-80): (10-30): (1-10). The composition takes a ribonucleic acid solution, tetrahydromethylpyrimidinecarboxylic acid and a spirulina maxima extract solution as core components, can play a better anti-light damage effect, and samples are sent to a third-party detection mechanism for zebrafish embryo light damage cell apoptosis test. The test result shows that the effect of the composition is better than the cell apoptosis effect of ergothioneine with the same concentration, and evaluation of the skin moisture content increasing effect shows that the composition can well repair and supplement the skin barrier; meanwhile, the invention further discloses application of the composition and cosmetics.

The invention belongs to the technical field of toxicity tests, and discloses a method for evaluating zebra fish developmental toxicity by single or combined use of fluopyram and tebuconazole. The method is simple, efficient and accurate, through a combined toxicity test and a single pesticide test, reference data is conveniently increased according to the influence of different environments and different medicaments on animals, the influence of pesticides on zebra fish development is calculated according to the death rate and the median lethal concentration of zebra fish embryos, the data is reasonably analyzed and processed, and the method is suitable for popularization and application. The problems that related evaluation standards formulated by depending on a single effect are not accurate and cannot truly reflect the change of the real environment quality are avoided, the environmental risk of effectively evaluating the pollutants in the environmental water body is improved, and an environmental threshold value safer for aquatic organisms is formulated conveniently.

The invention belongs to the technical field of experimental animal models, and particularly relates to a construction and analysis method of a zebra fish heart failure model. The invention discloses a construction method of a zebra fish heart failure model. The construction method comprises the following steps: S1, pretreating a zebra fish embryo; and S2, putting the zebra fish embryos pretreated in the step S1 into an exposure solution for exposure, thereby completing the construction of the zebra fish heart failure model. According to the construction and analysis method of the zebra fish heart failure model, accurate induction and dynamic monitoring of heart failure phenotypes are achieved through a standardized drug treatment process and a multi-mode heart function evaluation system, and an efficient platform is provided for cardiovascular drug screening and toxicity evaluation. The construction and analysis method of the zebra fish heart failure model further extends to the field of medicine and functional food heart protection efficacy evaluation, and objective grading authentication of food and medicine efficacy is achieved.

The invention belongs to the technical field of animal model construction, and particularly relates to a construction method and application of an arrhythmia zebra fish model with atrial enlargement and abnormal second leap second structure, the model is a cyth3ae6 / e6 mutant zebra fish, sgRNA of a sixth exon of a target zebra fish cyth3a gene is designed, the cyth3a gene of a wild type zebra fish embryo is mutated by using a sgRNA and Cas9 protein mixture, and the target zebra fish cyth3a gene is obtained. Hybridizing the obtained F0-generation zebrafish with wild-type zebrafish, screening out F1-generation heterozygotes, performing internal crossing on the F1-generation heterozygotes, and screening out positive homozygote F2-generation zebrafish, namely a zebrafish model; the cyth3ae6 / e6 mutant zebrafish is constructed through cyth3a gene specific knockout for the first time, and the model construction method is simple, high in efficiency and high in success rate; the method can be used for evaluating or screening drugs for treating arrhythmia cardiomyopathy.

The present invention provides the use of thyroid hormone and its analogs in the preparation of a drug for treating α-thalassemia. Specifically, the drug can be used to regulate ζ-globin gene expression. During K562 cell differentiation, the thyroid hormone analogs can specifically and significantly upregulate the expression of the ζ-globin gene (HBZ) by as much as 50 times. After treatment with thyroid hormone and its analogs in model animal zebrafish embryos, the expression of the ζ-globin gene (hbae5) can also be specifically upregulated by as much as 30-70 times. Therefore, the present invention uses thyroid hormone and its analogs to specifically activate the expression of the ζ-globin gene, that is, to reactivate the silenced ζ-globin gene in α-thalassemia patients to inhibit the destruction of red blood cells. This method provides a method for preparing a drug for treating α-thalassemia, provides an economical, safe and effective method for the treatment of α-thalassemia, and can be widely promoted and used.

The application belongs to the technical field of animal model construction, and relates to a construction method and application of a zebrafish aging model. The construction method comprises the following steps: 1, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein A and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-I is obtained, and a knock-in strain I is obtained through screening; 2, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein B and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-II is obtained, and a knock-in strain II is obtained through screening; 3, the knock-in strain I and the knock-in strain II are mated, zebrafish with fluorescent protein A and fluorescent protein B simultaneously expressed in the heart are screened from the offspring, and a wrn gene knockout homozygote, that is, a zebrafish aging model, is obtained. The construction method is more convenient to breed, significantly reduces breeding costs and the workload of screening and breeding, and has good application value.

The invention belongs to the technical field of gene engineering, and particularly relates to a construction method of a ribosomal disease zebrafish model. The invention provides a construction method of a ribosomal disease zebrafish model, which comprises the following steps: directionally mutating a pno1 gene in a wild zebrafish embryo to obtain F0-generation zebrafish; mating the F0-generation zebrafish with wild type zebrafish, and screening out positive heterozygote F1-generation zebrafish from birth zebrafish as the ribosomal disease zebrafish model. The zebrafish mutant is constructed through the targeted ribosomal factor gene, multi-system phenotypes of hematopoietic system defects, skeletal insufficiency and digestive system dysplasia can be reproduced at the same time, the problem that in the prior art, disease phenotypes are incomplete is solved, and the zebrafish mutant can be widely applied to pathogenesis research and drug screening of ribosomal diseases and has a wide application prospect. An important platform is provided for developing a novel treatment means.

The invention provides a construction method and application of an IgM gene knockout zebrafish antibody deletion model, and belongs to the technical field of zebrafish model construction.The method is based on a CRISPR gene knockout technology and specifically comprises the following operation steps that gRNA is synthesized for a CRISPR target located in a CH2 exon region of a zebrafish IgM gene, the gRNA and Cas9 mRNA / Cas9 protein are introduced into a wild type zebrafish embryo, and the zebrafish antibody deletion model is obtained. The P0-generation mutant and wild type zebra fish are hybridized to generate a heterozygote F1 generation, and then the heterozygote F1 generation is selfed to obtain a homozygote mutant, namely the IgM gene knockout zebra fish antibody deletion model. The invention provides the first IgM gene knockout zebrafish antibody deletion model, which is beneficial to exploring the biological function of the IgM gene and determining the influence of immunoglobulin antibody M synthesis disorder on the immune response of the zebrafish, and has application value for rapidly screening and evaluating vaccine adjuvant drugs and natural efficacy factors.

The present invention relates to the field of astaxanthin oil, specifically to a method for preparing astaxanthin oil with high anti-inflammatory activity and its application. The present invention cultivates Haematococcus pluvialis H7 and Haematococcus pluvialis ENN71 in a racetrack photobioreactor and a pipeline photobioreactor to accumulate astaxanthin and prepare astaxanthin oil. Under the same concentration and conditions, the combined culture significantly enhances the anti-inflammatory activity of astaxanthin oil compared to astaxanthin oil prepared by culturing Haematococcus pluvialis H7 or Haematococcus pluvialis ENN71 alone, increasing the zebrafish embryo neutrophil aggregation inhibition rate by 76.2% and 115.2%, respectively. The present application optimizes the ratio of the combined culture of Haematococcus pluvialis H7 and Haematococcus pluvialis ENN71. When the mass ratio of Haematococcus pluvialis H7 to Haematococcus pluvialis ENN71 is 1:2, the astaxanthin oil prepared by the combined culture has the highest anti-inflammatory activity, with a zebrafish embryo neutrophil aggregation inhibition rate of 77.1±0.36%.

This invention belongs to the field of animal model construction technology, specifically relating to a method and application for constructing a zebrafish model of arrhythmia with atrial enlargement and abnormal intercalated disc structure. This model is cyth3a. e6 / e6 A mutant zebrafish model was developed by designing an sgRNA targeting exon 6 of the zebrafish cyth3a gene. The cyth3a gene in wild-type zebrafish embryos was mutated using a mixture of sgRNA and Cas9 protein. The resulting F0 generation zebrafish were crossed with wild-type zebrafish, and heterozygotes in the F1 generation were selected. These heterozygotes were then inbred, and positive homozygotes in the F2 generation were selected, thus forming the zebrafish model. This is the first time that a cyth3a gene-specific knockout model has been constructed. e6 / e6 The mutant zebrafish model is simple, efficient, and has a high success rate; it can be used to evaluate or screen drugs for the treatment of arrhythmic cardiomyopathy.

The present invention discloses a method for constructing a zebrafish autism model, comprising the following steps: Step 1: Prepare zebrafish embryos: Use a standard zebrafish culture method to cultivate healthy zebrafish embryos; Step 2: After culturing the zebrafish embryos for 1 day, peel off the egg membrane with forceps; Step 3: Expose the zebrafish embryos to a dopamine inhibitor; the duration is 1 day to 5 days; Step 4: Observe and evaluate the effect: When the zebrafish embryos develop to 5 days, observe the motor ability and molecular markers to evaluate the formation of the autism model of the zebrafish embryos under the action of the dopamine inhibitor. After 21 days, observe the social ability to evaluate the reliability of the autism model. The present invention aims to provide a simple, easy-to-implement and stable method for constructing a zebrafish autism model, providing a new way for the progress of autism research and treatment.

This provides a rapid method for comprehensively measuring the toxicity of sewage. [Solution] The invention includes the steps of: (S1) measuring zebrafish embryo toxicity indices using water samples in the full process of a sewage treatment plant, including traditional toxicity indices and behavioral toxicity indices, and performing data preprocessing; (S2) establishing a comprehensive sewage toxicity prediction model based on different target variables, with traditional toxicity indices as target variables and behavioral toxicity indices as feature variables; and (S3) inputting zebrafish embryo behavioral toxicity indices data from water samples for measurement as input data into the prediction model, selecting a corresponding prediction model according to the prediction results of the target variables, and obtaining the comprehensive toxicity of the sewage samples for measurement. The present invention is suitable for rapid measurement of comprehensive toxicity in large quantities of actual sewage samples because it can obtain standardized comprehensive toxicity indices for actual sewage samples while maintaining the advantages of speed, ease of operation, and low cost.

The invention discloses application of transgenic zebrafish in preparation of an animal model capable of visually monitoring mast cells. The transgenic zebrafish is prepared by the following method: respectively carrying out XhoI and BamHI double enzyme digestion on a cpa5 gene promoter sequence (SEQ ID NO: 1) and plasmids with fluorescent protein genes and Tol2 transposon recognition sites, and then connecting to obtain recombinant plasmids; and then introducing the recombinant plasmid and Tol2 transposase mRNA into a zebra fish embryo in a microinjection mode in a 1-cell period of zebra fish embryo development, and culturing at constant temperature to obtain the transgenic zebra fish. The cpa5 promoter is constructed to drive an expression framework of the fluorescent protein, so that the mast cells naturally emit fluorescence in the zebra fish body, and an effective animal model is provided for mechanism research and drug screening of mast cell related diseases.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The invention discloses a construction method and application of an adriamycin-induced zebrafish osteoporosis-like model, and the construction method comprises the following steps: by utilizing the characteristics of transparency and rapid bone development of zebrafish embryos and juvenile fishes, selecting the zebrafish embryos of 2dpf, and culturing the zebrafish embryos to 6dpf in an adriamycin aqueous solution of 20 mu m, so as to obtain the adriamycin-induced zebrafish osteoporosis-like model. Therefore, the visual zebrafish osteoporosis-like model is efficiently constructed, juvenile fish bodies are transparent, imaging is visual, bone development changes can be observed in vivo in real time, and visual evaluation of bone development injuries is achieved. The built zebrafish osteoporosis-like model clearly shows the systematic inhibition effect of chemotherapy drugs on bone formation and mineralization, has the advantages of being short in building period, easy and convenient to operate, high in repeatability and sensitive to drug reaction, can be used for rapid dose screening and intervention experiments, and has good application prospects. The method has great application potential in research of pathogenesis of CIOP, screening of anti-osteoporosis drugs and evaluation of curative effect of the anti-osteoporosis drugs.

This invention discloses a method for extracting and preparing polysaccharides from the traditional Chinese medicine *Cat Ginseng* and its applications. The process involves drying and pulverizing the raw material, defatting it with 95% ethanol, and then using ultrasound-assisted extraction. The liquid-to-solid ratio, extraction time, and ultrasonic power are optimized using response surface methodology. The extract is then centrifuged, concentrated, precipitated with alcohol, deproteinized and decolorized using macroporous resin, dialyzed, and freeze-dried to obtain crude polysaccharides. These crude polysaccharides are then purified by DEAE-52 and Sephadex G-150 column chromatography to obtain three homogeneous new polysaccharide fractions: AvPs1-1, AvPs2-1, and AvPs2-2. The polysaccharide extract, dominated by AvPs2-1 and AvPs2-2, not only exhibits protective effects on zebrafish embryonic development but also demonstrates significant anti-fibrotic activity in a liver injury model. Due to its natural non-toxicity, water solubility, and multiple functions including embryonic development protection and anti-fibrosis, this polysaccharide shows broad application prospects in functional foods, health products, and therapeutic drugs.

This invention relates to a method for synthesizing ascorbate glucoside via yeast lysate and its application, belonging to the field of cosmetic technology. The invention discloses a Bifida ferment lysate, which is a fermentation product lysate of *Bifidobacterium longum*, wherein the *Bifidobacterium longum* is at least one of *Bifidobacterium longum* HJ-005, *Bifidobacterium longum* subsp. *CGMCC* 1.2186, and *Bifidobacterium longum* subsp. *infant* CGMCC 1.15639; the nucleotide sequence of the 16S rRNA of *Bifidobacterium longum* HJ-005 is shown in SEQ ID NO: 1. This invention uses a specific fermentation product lysate of *Bifidobacterium longum* to catalyze the transglycosylation reaction of vitamin C, obtaining a reaction product containing AA2G, thereby increasing the yield of AA2G, significantly reducing the preparation cost of AA2G, and significantly inhibiting the production of melanin in zebrafish embryos, exhibiting a whitening effect. It can be used as a cosmetic raw material without purification.

The invention relates to the modelling of a disorder called tubulinopathy, which refers to a wide range of cortical developmental malformations (CDM) resulting from defects in genes encoding tubulin proteins that regulate neuronal migration during brain development, by knocking out the tubulin gamma 1 (Tubg1) gene in zebrafish (Danio rerio) embryos using CRISPR / Cas9 technology. The transgenic animal in question allows the investigation of the phenotype and molecular pathological mechanisms of CDMs resulting from the deficiency of the Tubg1 protein during the developmental process. In the invention, the region located in the third exon of the Tubg1 gene in zebrafish is targeted and knocked out with a guide RNA having the nucleotide sequence SEQ ID NO: 1.

The invention belongs to the field of animal models, and particularly relates to a zebrafish embryo model for 5-FU side effect research and a construction method and an evaluation method thereof. The construction method comprises the following steps: selecting zebrafish embryos for later use; and putting the zebrafish embryo into a culture hole, and then adding 5-FU for modeling treatment, thereby finishing the construction of the zebrafish embryo model for 5-FU side effect research. The 5-FU induced intestinal mucositis model is constructed by adopting a 3-5-day zebrafish embryo and analyzing information such as the survival rate, the travel trajectory, the intestinal morphology and ROS fluorescent living imaging of zebrafish, and the 5-FU induced intestinal mucositis model has the advantages of being simple in observation index, easy to observe, simple to operate, capable of saving operation time, convenient, rapid, high in efficiency and the like. The method has the characteristics of simple operation, high cost, cheaper mouse model, qualitative and quantitative properties and the like, and is suitable for high-throughput screening of 5-FU induced intestinal mucositis model drugs.

The invention discloses a method for detecting biotoxicity of refinery wastewater based on dual levels of zebra fish embryos. The method comprises the following steps: S1, analyzing a death rate; s2, oxidative stress level analysis: respectively determining the ROS content and the SOD content of the zebrafish embryos by adopting an ROS kit and an SOD kit so as to determine the oxidative stress level of the zebrafish embryos; and S3, carrying out gene transcriptomics analysis. The invention provides a detection method for comprehensively and effectively determining the biotoxicity of the refinery wastewater by taking omics analysis as a technical means and combining determination of death rate of zebra fish embryos, morphological deformity occurrence condition and oxidative stress level, and a most scientific and reasonable detection result is finally obtained layer by layer progressively. The experimental process has the characteristics of short time period, intuitive and comprehensive experimental result and the like, and can truly reflect the biotoxicity of the refinery wastewater.

The invention relates to a construction method of a congenital pure red blood cell aplastic anemia zebrafish model, which comprises the following steps: directionally mutating a bysl gene in a wild type zebrafish embryo to obtain F0 generation zebrafish; mating the F0 generation zebrafish with wild type zebrafish, and screening out positive heterozygote F1 generation zebrafish from the born zebrafish; mating the positive heterozygote F1-generation zebrafish with wild type zebrafish, and screening out positive heterozygote F2-generation zebrafish from the born zebrafish; and hybridizing the male and female positive heterozygote F2-generation zebrafish, and screening positive homozygote zebrafish from the born zebrafish as the congenital pure red blood cell aplastic anemia zebrafish model. The model can simulate the symptoms of the congenital pure red blood cell aplastic anemia, and can be used for mechanism research, drug evaluation and new drug screening of the congenital pure red blood cell aplastic anemia.

The application discloses a kind of based on the analysis method and system of melanin content of migration control, and the present application relates to the technical field of quantitative analysis method, and along the illumination parameter is photographed to zebra embryo, and multiple images of different angles of zebra embryo are collected;According to multiple images, corresponding stereoscopic data are determined, and time series three-dimensional model is generated according to the alignment of the stereoscopic data and time point data, to ensure the accuracy of time series three-dimensional model.Therefore, melanin area is determined based on the identification of time series three-dimensional model, and melanin three-dimensional volume is determined according to the area position of melanin area and the voxel statistics of melanin area;The migration path of melanin area is collected, and the quantitative analysis of melanin content is triggered according to the migration path of melanin area, the migration speed of melanin area and melanin three-dimensional volume, to ensure the accuracy of quantitative analysis of melanin content.

This invention belongs to the field of biomedical technology, specifically disclosing the application of mancozeb in the preparation of ERK inhibitors. Through zebrafish embryo models and HEK293T cell experiments, this invention demonstrates that mancozeb can specifically inhibit the MAPK / ERK pathway as an ERK inhibitor, and that at concentrations of 2μM to 8μM, it can induce a three-dimensional spiral notochord malformation in zebrafish embryos similar to that caused by 100μM U0126 treatment, providing a new approach for the preparation of MAPK / ERK pathway inhibitors.