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Method for applying zebra fish to test toxicity of organic solvent

An organic solvent, zebrafish technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as long method cycle, and achieve the effect of shortening detection cycle

Inactive Publication Date: 2013-01-30
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method cycle that above-mentioned two prior art reports is all longer

Method used

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  • Method for applying zebra fish to test toxicity of organic solvent
  • Method for applying zebra fish to test toxicity of organic solvent
  • Method for applying zebra fish to test toxicity of organic solvent

Examples

Experimental program
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Embodiment 1

[0031] The screening of embodiment 1 optimal embryo culture medium

[0032] 1.1 The preparation of different formulas of embryo culture medium and the effect of culturing zebrafish

[0033] A Prepare embryo culture solution containing the following concentrations of inorganic salts with ordinary water, adjust the pH to 7.2, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 1:

[0034] Table 1 Effects of embryo culture medium prepared with ordinary water on zebrafish embryos

[0035]

[0036]

[0037] B Use weakly alkaline water with a pH of 7.2 prepared by electrolysis of pure water to prepare an embryo culture solution containing the following concentrations of inorganic salts, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 2:

[0038] Table 2 Effects of embryo culture medium prepared ...

Embodiment 2

[0041] Example 2 Zebrafish Toxicity Assay

[0042] The above-mentioned embryo culture fluids of numbers A1 and B1 were used to prepare organic solutions with graded concentrations, and the solutions were subjected to the zebrafish toxicity test, wherein each treatment was repeated 5 times, and the average value was taken, and the control was the embryo culture fluid without solvent A1 and B1. The determination is mainly carried out through the following steps:

[0043] 1) The cultivation of zebrafish:

[0044] The male and female common zebrafish of the sexually mature AB line were separated into separate tanks, and were raised in a circulating water culture system, with 14 hours of lighting and 10 hours of darkness alternately, the water temperature was kept at 28°C, and brine shrimp artificially incubated for 24 hours were regularly fed;

[0045] 2) Embryo selection:

[0046] The day before egg collection, sexually mature zebrafish were placed in a mating tank at a ratio ...

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Abstract

The invention relates to a method for applying zebra fish to test toxicity of an organic solvent. The method comprises the following main steps of: preparing an embryo culture solution, then adding the organic solvent to the embryo culture solution, ensuring that the concentration of the organic solvent in the embryo culture solution reaches the concentration needed by measuring, using the embryo culture solution to which the organic solvent is added to culture zebra fish embryos and then observing and recording the developmental aberration rates and mortality of the zebra fish embryos in 6-12 hours to judge the toxicity of the organic solvent toward the embryos, wherein the embryo culture solution is directly prepared from alkalescent water with pH value being 6.7-8.5; and the embryo culture solution contains 5mM of NaCl, 0.17mM of KCl, 0.43mM of CaCl2 and 0.33mM of MgSO4. The method has the following beneficial effect that the embryo culture solution prepared from the alkalescent water ensures that the zebra fish embryos can quickly test the toxicity of the organic solvent, thus shortening the detection period.

Description

technical field [0001] The invention relates to the field of toxicity detection, in particular to a method for testing the toxicity of organic solvents using zebrafish. Background technique [0002] Most drugs in the prior art have poor water solubility, so a certain amount of solvent is often required in the preparation of dosage forms. However, as a chemical substance, solvents also have certain potential threats to humans or animals, and a large amount of intake can also affect the health of humans or animals. Therefore, it will be particularly important to quickly detect the toxicity of solvents and formulate their maximum usage limits. [0003] Zebrafish is a novel model organism. The live zebrafish screening model has many advantages, and it is an excellent experimental model fish to replace frogs, fruit flies, and mice as research objects. Zebrafish are cheaper to feed and maintain, require less space and are easy to reproduce indoors on a large scale. The length o...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 陈峰裴端卿粱桂婵周兴茹舒晓东
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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