22 results about "Brine shrimp" patented technology

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The present invention relates to a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, and, more specifically, to: a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, the method feeding spirulina and Haematococcus to hatched brine shrimp to prepare a tropical fish feed, and thus can promote the growth of tropical fish, boost the immune system and improve the consumption, digestion, absorption and palatability for tropical fish.

The utility model discloses an acete chinensis length measuring tool, which relates to the technical field of acete chinensis research and comprises a measuring disc, a plurality of measuring grooves are arranged on the measuring disc, each measuring groove can accommodate a single-tail acete chinensis sample, and a length scale layer is arranged in each measuring groove along the length direction so as to measure the length of the corresponding acete chinensis sample. According to the utility model, the lengths of acete chinensis samples can be measured in batches, and the measurement efficiency and the measurement accuracy are improved.

The application discloses a disease prevention and multi-nutrition strengthening method for biological bait of blue crab breeding, which is applied to biological bait of the blue crab during the period from the nauplius to the zoea, and technical scheme points include disease prevention and multi-nutrition strengthening of rotifer and disease prevention and multi-nutrition strengthening of brine shrimp nauplius. Through multiple disinfection and multi-nutrition strengthening of the living biological bait, especially the rotifer and the brine shrimp nauplius, diseases of the crab seedlings caused by the biological bait with disease bacteria are prevented, and meanwhile, nutrition substances in the biological bait are specialized according to the growth and development needs of the crab seedlings, so that good growth and development of the crab seedlings are ensured.

This invention provides a method for the recovery of seahorses with bulging eyes. Algae, bacteria, and light are the three key factors causing bulging eyes. The recovery is achieved through the following three steps: A) Pond removal: The entire pond of seahorses exhibiting bulging eyes is removed, soaked in fresh water, and then placed in a clean new pond to reduce the impact of excessive bacteria and algae in the original pond; B) New pond setup: A shade net is installed above part of the new pond, and several attachment devices are placed directly below it to reduce the impact of light; C) Breeding management: After being placed in the new pond, the seahorses are fed once a day in the morning with brine shrimp soaked in pathogen inhibitors; frozen mysids are fed at two-hour intervals and seven and a half-hour intervals. A slight water flow is maintained throughout the breeding period, and aeration is kept on except when feeding and cleaning; Once the seahorses' eyes return to normal, feeding with brine shrimp is stopped, but the shade net is retained.

The utility model relates to the technical field of brine shrimp treatment, in particular to a brine shrimp hatching and collecting device which comprises a box body, a filtering tank and a waste water tank which are sequentially connected from top to bottom, a filtering plate is arranged in the filtering tank, a water outlet is formed in the lower portion of the waste water tank, a water inlet is formed in the upper portion of the box body, and a PH detector is arranged in the box body. A heating assembly is further inserted into the box body, a shrimp inlet, a salt inlet and an alkali inlet are formed in the side wall of the box body, the shrimp inlet is connected with shrimp storage equipment through a shrimp inlet pipe, the salt inlet is connected with salt storage equipment through a salt inlet pipe, the alkali inlet is connected with alkali storage equipment through an alkali inlet pipe, and a partition plate is further arranged in the box body; the whole device further comprises a controller in signal connection with the PH detector, the heating assembly, the shrimp storage device, the salt storage device and the alkali storage device, and the controller is used for controlling all the devices to add shrimps, water, salt and alkali into the box body. The device effectively solves the technical problems that an incubation device is single in adjusting means, the shrimp incubation requirement cannot be met, and the incubation efficiency is low.

The invention develops the method for inducing and controlling the propagation of the tea bark beetles by utilizing the sex pheromone, so that the sex pheromone of the tea bark beetles can be quickly obtained at high yield, and the obtained sex pheromone can be effectively utilized to induce and control the propagation of the tea bark beetles. The tea bark beetles are collected from rotten wood in an electric shock desinsectization mode for the first time. The low temperature of 16 DEG C, the high humidity of about 88%, low-concentration alcohol stimulation and blue light irradiation are selected, and rapid incubation of the tea bark beetle eggs is achieved. According to the method, high light with low temperature of 18 DEG C, high humidity of about 86% and 1200 Lux is selected for stimulation, so that rapid eclosion of the tea bark beetle pupae is realized. Alkali stimulation of 120 mg / L sodium hydroxide at the high temperature of 32 DEG C and the long photoperiod of 20 L: 4 D is selected, and nutrition enhancer fairy year eggs are added, so that the yield of sex pheromones is increased.

The invention belongs to the technical field of molecular biology, germplasm resource science and molecular breeding, and discloses an artemia 5K liquid phase chip based on a targeted capture sequence, a preparation method and application thereof, and the liquid phase chip comprises 4329 SNP sites. Compared with a previous fingerprint spectrum developed based on SNP molecular markers, the artemia liquid-phase chip disclosed by the invention contains more SNP markers, the identification accuracy is higher, the later detection cost is lower, the design is flexible, and interested marker sites can be added at any time in the later period.

The invention belongs to the technical field of hermatypic coral cultivation, and particularly relates to a hermatypic coral asexual cultivation method based on hydranth separation and reattachment. According to the method provided by the invention, natural separation of the hydranth is induced, the defects of mechanical stripping or chemical induction are avoided, the separation rate of the hydranth is high, and the separated hydranth is high in form integrity rate and activity, so that a foundation is laid for subsequent reattachment. According to the method, the adhesion rate of the hydranths is increased through the pretreated adhesion matrix, the survival rate of the hydranths is maintained at about 80% after the hydranths are bred for 60 days in combination with appropriate environmental parameters and accurate feeding, and the period of the hydranths from being separated to being bred into transplantable larvae is greatly shortened through the specific treatment method. Meanwhile, complex equipment or expensive chemical reagents are not needed in the whole cultivation process, brine fairy shrimps easy to cultivate are adopted as bait, the source is wide, the cost is low, the method is suitable for large-scale application and popularization, a feasible technical scheme is provided for large-scale asexual cultivation of hermatypic coral, and the method has important significance on restoration and protection of a coral reef ecological system.

The present application provides a kind of dish ware for quickly separating and counting brine shrimp eggs, including dish ware body, the dish ware body includes bottom disc and the sieve tray that can be installed on bottom disc and can rotate in bottom disc, the bottom disc includes disc body, the central position of the disc body is equipped with bearing, the sieve tray includes support, the central position of the support is equipped with mesh screen for separating and counting brine shrimp oocyte, the mesh screen includes four fan-shaped areas of same size;The present application has the following beneficial effects: the present application can separate brine shrimp oocyte from each other and uniformly distribute in culture dish without damaging the appearance structure of brine shrimp oocyte, so that the fertility can be quickly and effectively counted, not only reduces the experimental time cost and labor cost, but also provides a basis for obtaining uniformly distributed brine shrimp oocyte image when measuring egg diameter of subsequent brine shrimp oocyte, so as to ensure the accuracy of egg diameter measurement.

The invention discloses a method for producing all-male largemouth micropterus salmoides based on soaking induction of sex reversal of largemouth micropterus salmoides, and particularly relates to the field of artificial propagation of all-male largemouth micropterus salmoides, the method comprises the following steps: S01, taking fairy shrimps as initial feed when largemouth micropterus salmoides fries obtained by incubation swim flatly; s02, transferring the largemouth micropterus salmoides fry into a water body in which a sexual reversal induction reagent is dissolved, keeping feeding three times every day during the period, and soaking and breeding the fry for 60 days; and S03, after soaking and culturing for 60 days, transferring the largemouth bass fry into a conventional culture water body for culturing, and after sexual maturity of the largemouth bass fry, obtaining a male population capable of generating seminal fluid, namely the all-male largemouth bass. According to the method, largemouth bass fries are soaked in a sex reversal induction reagent with a specific concentration, sex reversal fishes with a high survival rate and a low malformation rate can be directly obtained, and then large-scale cultivation of all-male largemouth bass is achieved.

The invention relates to blunt-snout bream terminal deoxynucleotidyl transferase and application thereof, and belongs to the technical field of gene engineering. The invention provides Branchiostoma belcheri terminal deoxynucleotidyl transferase (BbeTdT). The amino acid sequence of the Branchiostoma belcheri terminal deoxynucleotidyl transferase (BbeTdT) is shown as SEQ ID NO: 2 or SEQ ID NO: We find that terminal deoxynucleotidyl transferase (TdT) derived from Branchiostoma belcheri not only has terminal transferase activity under in vitro conditions, but also has nucleotide preference different from that of TdT of vertebrates. The BbeTdT has better selectivity aiming at a specific sequence or a terminal structure, and non-specific addition can be reduced in a practical application process, so that complementation with mammal TdT is formed, and the BbeTdT is suitable for industrial processes needing different preferences. In addition, the BbeTdT mutant is constructed, the activity of the terminal transferase of the BbeTdT mutant is higher, and the engineering transformation potential of the BbeTdT is further optimized.

The invention relates to the technical field of aquaculture, and discloses an industrial aquaculture workshop shell shrimp larva metamorphosis development cultivation method, which comprises the following steps: S1, when zoeae enters a metamorphosis development critical window, carrying out hypotonic period stress pretreatment on an aquaculture water body; s2, changing the water body, and adding a water body environment synchronous inducer composed of an ecdysone synthesis key precursor, a GABAergic nerve inhibitor and a chitin synthesis unit at one time; s3, then forcibly stopping feeding the larvae, and then feeding fortified baits; s4, after the larvae are intensively metamorphosed and developed into opossum shrimp larvae, changing a large amount of water to reset the culture environment. Through combination of environmental factor pretreatment, physiological function pulse type induction and a specific nutrition recovery strategy, accurate regulation and control of the larva metamorphosis process are achieved, metamorphosis development synchronism is remarkably improved, the cultivation period is shortened, and the survival rate is remarkably increased.

The invention discloses a cultivation method for preventing skin diseases of hippocampus kelloggi larvae, and belongs to the technical field of aquaculture. The method comprises the following steps: a bait preparation step: mixing artemia nauplii and copepods to prepare composite bait; disease prevention and management: adding a zanthoxylum nitidum water extract into the water body; and a special bait feeding step: taking the compound bait as a unique bait source for feeding, and preventing skin diseases through a zanthoxylum nitidum aqueous extract during the feeding process. The method solves the technical bottlenecks of low survival rate, abnormal intestinal development, difficulty in domestication and bacterial infection of the hippocampus kelloggi larva in the mouth opening stage. Experiments show that the method can significantly improve the survival rate and growth performance of the hippocampus kelloggi larvae and improve the health state of the hippocampus kelloggi larvae. On one hand, by promoting intestinal development, the digestion and absorption functions and the immune level of the body are effectively improved; on the other hand, common diseases such as skin ulcer and tail rot caused by bacterial infection can be obviously prevented, and a reliable guarantee is provided for healthy breeding of the hippocampus kelloggi.

1. Name of the designed product: packing box (double sauce of brine shrimp). 2. Use of the designed product: the designed product is used for product packing box. 3. Design points of the designed product: in shape. 4. Picture or photo showing the design points most: assembly 1 perspective view 1.

The utility model discloses an intelligent hatching device for aquaculture bait, which comprises a rack, a hatching pond, a constant temperature device, a water oxygenation device, a water inlet device and a shrimp discharge device, the hatching pond is fixed on the rack, the water inlet device is communicated with a water inlet of the hatching pond, the shrimp discharge device is communicated with a water outlet of the hatching pond, and the hatching pond is fixed on the rack. The constant temperature device is arranged on the inner wall of the hatching pond, and the water oxygenation device is communicated with the inside of the hatching pond. By means of the mode, the temperature of the hatching water body can be automatically adjusted, the oxygen content in the water body can be greatly improved by means of oxygen supply from bottom to top, the hatching rate of baits (brine shrimps) is greatly improved, and meanwhile it is guaranteed that fresh baits can be hatched in different time periods by means of the multiple sets of independent hatching ponds.

This invention belongs to the fields of molecular biology, germplasm resource science, and molecular breeding technology. It discloses an artemia 5K liquid-phase chip based on targeted capture sequences, its preparation method, and its applications. The liquid-phase chip includes 4329 SNP sites. Compared to previous fingerprinting based on SNP molecular markers, the artemia liquid-phase chip of this invention contains more SNP markers, has higher identification accuracy, lower post-detection costs, and is more flexible in design, allowing for the addition of marker sites of interest at any time.

The scheme discloses a zebrafish full-automatic precision feeding breeding system and a use method thereof, and comprises a worm breeding unit for hatching brine shrimp eggs; and a feeding unit for feeding the brine shrimp eggs bred by the worm breeding unit into a breeding container of zebrafish. The brine shrimp eggs can be automatically bred, and the zebrafish can be bred through the brine shrimp eggs, so that the breeding effect is obviously improved and the cost is reduced. The system has the ability of stable self-purification and sewage reduction, reduces the artificial breeding maintenance cost, and improves the stability and durability of the breeding environment.

The invention discloses a breeding method of onychostoma macrolepis. The breeding method comprises the following steps: S1, selecting parent fishes; s2, the selected parent fishes are fed into a breeding pond to be cultured, and feed with crude protein larger than or equal to 35% is fed; s3, preparing oxytocin to inject the female parent fish and the male parent fish in the breeding season; s4, artificial insemination: mixing eggs with semen, standing for 1 minute, and adding clear water for activation; s5, fertilized eggs are disinfected and then put into an incubator to be hatched in running water; and S6, cultivating larvae, and feeding artemia nauplii and microparticle compound feed. Compared with the prior art, the breeding method has the advantages that operation and use are convenient, and large-scale production and use can be achieved.

The invention discloses a brain network neural unit recognition system based on uniform design and a krill swarm algorithm, belongs to the technical field of brain science and computational intelligence cross, and comprises a recognition unit and a recognition method. The identification unit comprises a data acquisition and preprocessing module, a uniform initialization module, a krill position updating module, a uniform design crossing module, a fitness evaluation and updating module and a result output module, and the data acquisition and preprocessing module is used for acquiring brain network data, preprocessing the brain network data, and outputting the preprocessed data to the krill position updating module; and obtaining a connection relationship between the neural units. According to the method, the global uniform search capability of uniform design and the efficient optimization capability of the krill swarm algorithm are creatively combined, so that the three core pain points of easy local optimum, unstable result and slow convergence speed in the existing brain network module identification technology are successfully solved; and a technical means with excellent performance is provided for basic research of brain science and clinical auxiliary diagnosis of neuropsychiatric diseases.

The penaeus japonicus larva breeding system comprises an outer-layer tank (1) and an adjusting lamp (2), and is characterized in that the adjusting lamp (2) is arranged on the rear side face of the outer-layer tank (1), a hook (3) is arranged on the top of the outer-layer tank (1), the bottom of the hook (3) is fixedly connected with a parent penaeus japonicus grid (4), a juvenile penaeus japonicus grid (5) is arranged in the outer-layer tank (1), and the juvenile penaeus japonicus grid (5) is arranged in the outer-layer tank (1). The juvenile shrimp grids (5) are fixedly connected with bolting silk filtering assemblies (6), a water pipe (13) penetrates through the rear side faces of the juvenile shrimp grids (5), and a water pump (21) is arranged at the other end, away from the juvenile shrimp grids (5), of the water pipe (13). Rotary water flow is formed in the juvenile shrimp grids, the phenomenon that larvae of shrimps sink to the bottom and accumulate is avoided, redundant seawater can be discharged from the bolting-silk filtering assembly, the bolting-silk filtering assembly can prevent bait organisms from overflowing, the bait density is high, and meanwhile the water health of the breeding device is guaranteed.

The present application provides a kind of based on broadband acoustic multi-feature fusion's Caridina multidentata echo identification method, comprising: collecting original broadband echo data, and simultaneously carrying out net sampling;Original broadband echo data is preprocessed;Dual-frequency frequency difference feature is extracted to candidate echo unit;Time-frequency feature is extracted to candidate echo unit;Candidate echo unit is mapped as acoustic image target, and morphology feature and texture feature are extracted;Multi-feature fusion and classification model training are carried out;The multi-dimensional feature vector of the candidate echo unit to be identified is input into the classification model, and the Caridina multidentata echo identification result is output, and the Caridina multidentata echo identification result is post-processed, to obtain the final Caridina multidentata echo distribution result.The present application can significantly improve the accuracy and stability of Caridina multidentata echo identification under the background of multi-species habitat, and is suitable for Caridina multidentata resource monitoring, fishery management and nuclear power cold source disaster-causing organism monitoring.