The invention discloses a refined synthetical culturing method for Cuora flavomarginata. The method comprises the following steps: 1) parent turtle: a, identifying the parent turtles; b, raising the parent turtles; c, mating of the parent turtles; d, spawning and hatching; 2) childish turtle: a, earlier-stage raising of the childish turtles; b, later-stage raising of the childish turtles; c, overwintering of the childish turtles; 3) young turtle: a, breeding; b, feeding baits; c, daily management; d, overwintering of the young turtle; and 4) adult turtle: a, breeding; b, feeding baits; c, daily management; d, overwintering of the adult turtle. The culturing method is capable of improving the survival rate of the Cuora flavomarginata to 90%, greatly reducing the death rate of the childish turtle period and the young turtle period, and performing the comprehensive culture from the source, and has the characteristics of high parent turtle mating efficiency, high germ cell quality, scientific management, high adult turtle quality, high viewing value, and high pharmaceutical value.