70 results about "Sepiella inermis" patented technology

The invention discloses a precision pond culture method for sepiella maindroni de Rochebrune. The precision pond culture method includes splashing 75 kilogram/mu of quicklime water at the bottom of a pond to comprehensively disinfect the pond and kill harmful organisms, laying black and thick mulching films on the pond and the periphery of the pond completely after the pond is disinfected, configuring an aerator, and seed breeding, wherein the breeding density is determined according to individual sizes of seeds, 400-450 seeds with trunk lengths ranging from 1.2 centimeters to 2.5 centimeters can be bred in each square meter of the pond, and 300-350 baby sepiella maindroni de Rochebrune with the trunk length ranging from 3.0 centimeters to 5.5 centimeters can be bred in each square meter of the pond. The precision pond culture method has the advantages that fast growth of the sepiella maindroni de Rochebrune can be promoted by means of adding compound microbial fertilizers, the EPA (eicosapentaenoic acid) content is increased, sepiella maindroni de Rochebrune diseases are reduced, the quantity of sepiella maindroni de Rochebrune which dies of diseases is reduced, beneficial microorganisms in water can be increased, and both the final quality and the quality are improved.

The invention belongs to the technical field of sea culture and particularly relates to a method for feeding sepiella maindroni. The method comprises steps of parent temporary culture feeding and seedling culture feeding. The parent temporary culture feeding comprises steps of temporarily culturing matched sepiella maindroni in an indoor cement pool and feeding the sepiella maindroni by using artemia larvae, vitamin, shrimps and minerals alternately at one-hour intervals in the temporary culture period. The seedling culture feeding comprises steps of feeding feed within 24 hours from seedling hatching, wherein the feed consists of raw materials such as basic materials, white fish meal, microcrystalline cellulose, dextrin, biological extract oil, the artemia larvae, the shrimps and flavones, and feeding pulpy feed to net boxes used for culturing the sepiella maindroni at five-time intervals each day. By the aid of the method, reasonable nutrition requirements of the sepiella maindroni can be met, the good intake effect is achieved, the feed cannot be accumulated excessively in the water, the sepiella maindroni can grow quickly and the survival rate of the sepiella maindroni is high and can reach more than 43%.

The invention discloses a method for feeding Sepialla maindroni with soft formula feed, which is characterized in that through preparing the formula feed powder needed to feed everyday, the formula feed powder is prepared into the long rod shaped soft formula feed with the length of 2 to 6 cm and the diameter of 0.2 cm, where the water content being 10% to 20%, the weight percentage of crude protein being 38% to 45% and the weight percentage of crude fat being 9% to 11%; and the long rod shaped soft formula feed is reserved with moisture retention and is evenly fed into a net cage of culturing the Sepialla maindroni for average three times a day with the everyday feeding weight being 8% to 12% of the total weight of the Sepialla maindroni in the net cage. The method has the advantages that survival rate of the Sepialla maindroni is high and reaches to more than 43%; the Sepialla maindroni grows well; benefits of artificial culture of the Sepialla maindroni are improved; and feed cost and man-hour cost for culturing the Sepialla maindroni are reduced.

The invention discloses a fluorescent labeling method for the inner shell of Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. In addition, a mode of staining solution injection is adopted, the injection is carried out at an acute angle, thus, the staining solution can be uniformly distributed on the inner shell favourably, and the invention is especially suitable for Sepiella. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

The invention relates to agricultural seawater aquaculture techniques and discloses a method for increasing fry breeding survival rate of Sepiella maindroni de Rochebrune. The method comprises the following steps: (1) fertilized egg incubation; (2) larva feeding; (3) fry-separated culture; (4) fresh and live feedstuff feeding acclimatization; and (5) later-stage staged-temperature-control raising. According to the method disclosed by the invention, in view of physiological characteristics of the Sepiella maindroni de Rochebrune, the acclimatization of chilled miscellaneous shrimps can be achieved in 5 days, the phenomena of batch-size death and self-mutilation do not occur, and the fry breeding survival rate can be increased to 50% from 20%. The method disclosed by the invention has the characteristics of simplicity and convenience in use, economical efficiency and practicability, and the fry breeding survival rate of the Sepiella maindroni de Rochebrune is effectively increased.

The invention discloses a cuttle-fish artificial insemination and incubation method and special agent thereof, wherein the eggs in oviduct of the female cuttle-fish are transferred into a container with nutrient solution to perform external insemination and isolated culture and an agarose layer is set between the container bottom and eggs; the nutrient solution contains the fresh or freeze-dry oviduct gland secretion of the female cuttle-fish. The special agent contains culture container bottom liner and nutrient solution and the culture container bottom liner is 0.2% of agarose and the nutrient solution contains 1-10 portions by weight of blood serum and 1000 portions by weight of sterile sea water. Because the molecular structure of agarose is loose, the nutrient material can freely flow and penetrate, the eggs on the agarose liner has little distortion without nutrition deficiency of the contact surface, The nutrition is provided by the oviduct gland secretion and the blood serum of low concentration, the eggs do not distort, thus the whole development process of the cuttle-fish embryo cultured in vitro is greatly accomplished.

The invention discloses environment-friendly nutritional type sepiella maindroni mixed feed which is prepared from the following components in parts by weight: 10%-25% of imported fish meal, 20%-40% of domestic fish meal, 10%-20% of soybean meal, 4%-10% of peanut meal, 10%-20% of strong flour, 5%-15% of alpha-starch, 2%-6% of fish oil, 2%-6% of soybean oil, 1%-5% of soybean lecithin oil, 2%-8% of sepia paste, 0.5%-2.5% of calcium dihydrogenphosphate, 0.2%-1% of vitamin premix, 0.5%-2.5% of mineral substance premix, 0.02%-0.2% of antioxidant, 0.05%-0.25% of choline chloride and 0.02%-0.05% of mildew preventive, totaling 100%. The invention aims to provide the environment-friendly, nutritional and efficient sepiella maindroni mixed feed and a preparation method thereof.

The invention discloses a subcutaneous fluorescent labeling method for Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. A special labeling reagent for the subcutaneous fluorescent labeling method for the Sepiella maindroni contains one portion of alizarin complexometric indicator powder and four to eight portions of yoghurt according to weight portion and forms a label in a subcutaneous area which is relatively concentrated, and the label has higher identifiability than a general staining label. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

The invention discloses a culture method capable of improving the cuttlebone medicinal value of sepiella maindroni. The method comprises the following steps: temporary parent culture, seedling raising, larva culture and adult culture, wherein adding the step of screening the amino acid component of the parent mantle for the temporary cultured sepiella maindroni in the step of temporary parent culture in the culture process so as to select the parent sepiella maindroni with the cuttlebone rapid proliferation potential; in the step of larva culture, feeding the sepiella maindroni larva with the artemia or copepoda which is strengthened by the amino acid relevant with cuttlebone proliferation; and in the step of adult culture, feeding the sepiella maindroni adult with the fodder which contains the amino acid relevant with cuttlebone proliferation. By supplying the relevant ammonia acid greatly needed by the cuttlebone growth, the method can ensure the nutritional need for the endoskeleton growth of the sepiella maindroni, in particular the synthesis need for the biomineralization regulatory protein. The method can use up the aspartic acid in the sepiella maindroni to promote the mineralization. The method has the advantages of simple steps, remarkable effects, and suitability for large-scale generalization.

The invention discloses a sepiella maindroni basal bait formula which contains the following components in parts by weight: 74 parts to 85 parts of aquatic product meat emulsion, as well as 4.2 parts to 5.8 parts of casein, 0.6 part to 1.8 parts of sodium chloride, 0.42 part to 0.46 part of threonine, 1.02 parts to 1.36 parts of glutamic acid, 1.72 parts to 2.04 parts of proline, 0.1 part to 0.2 part of valine, 0.26 part to 0.35 part of isoleucine, 0.3 part to 0.6 part of leucine, 0.7 part to 0.9 part of tyrosine, 0.5 part to 0.8 part of lysine, 1.2 parts to 1.3 parts of arginine and 0.2 partto 0.6 part of tryptophan. The aquatic product meat emulsion is fish meal or shrimp chaff or combination of the fish meal and the shrimp chaff. The sepiella maindroni basal bait has the advantages ofcomprehensive nutrition, good feeding attraction property, low bait coefficient, low cost and convenience in manufacturing.

The invention relates to a method for quickly and efficiently detecting a sepiella maindroni population by using environmental DNA to detect the sepiella maindroni population without damaging the environment and the species population. Collected eDNA samples are taken from the soil, sediment and water and the like, no environmental burden is caused to detection points, and no adverse influence iscaused to the living environment of the species. Special primers and probes are utilized to accurately, quickly and efficiently detect the DNA of sepiella maindroni, the efficiency and accuracy of thedetection are improved, and the detection cost is reduced. Compared with a field survey method used by the transmission of the sepiella maindroni population distribution detection, the method has theadvantages of saving time and labor, being low in cost and efficient and causing no harm to target species and no damage to a survey point ecosystem.

The invention relates to the technical field of squid breeding, and particularly relates to a device and operation method for over-summer relay preservation of sepiella maindroni germplasm. The devicefor over-summer relay preservation of sepiella maindroni germplasm comprises a breeding box; a thermal-insulation water-insulation wall is arranged in the breeding box; the thermal-insulation water-insulation wall divides the inner part of the breeding box to a squid parent spawning pond and a fertilized egg hatching pond; the box wall of the breeding box is provided with a water circulation device; the water circulation device comprises a water inlet pipe and a water outlet pipe; the water inlet pipe is connected with the water outlet pipe through a water return pipe; the water return pipe is provided with a water stop valve, a filter, a high-pressure pump, a water temperature regulator and a salinity regulating device; a floating object is arranged on the pond water surface of the squidparent oviposition pond; the floating object is penetrated by a feeding hopper; the bottom of the feeding hopper is provided with a material through tube; two sides of the material through pipe are provided with discharge pipes; and the material through pipe is provided with egg-picking attaching strips. According to the invention, the survival rate of squid breeding is ensured, so that squid fertilized eggs and larvae can smoothly live through summer.

The invention belongs to the technical field of mariculture, and particularly relates to a formula feed for feeding sepiella maindroni. The formula feed is prepared from the following raw materials in percentage by weight: 20-25 percent of wheat flour, 8-10 percent of corn DDGS, 15-18 percent of dried-powder of housefly larvae, 12-14 percent of albumen powder, 3-5 percent of beer yeast, 1-2 percent of DHA microalgae oil, 1 percent of spirulina, 2 percent of fish oil, 1 percent of licorice root powder, 5-8 percent of dried cabbage powder, 1-2 percent of black soybean meal, 0.1-0.2 percent of choline chloride, 0.2 percent of sodium chloride, 0.04-0.05 percent of L-carnitine, 2-4 percent of isomaltooligosacharide, 0.3 percent of compound bacillus, 0.05-0.06 percent of a mildew-proof agent, 1-3 percent of compound adhesive, 1-2 percent of compound mineral, 0.5-1.5 percent of vitamin complex and the balance of dregs of codonopsis pilosula. The formula feed is reasonable in ingredient formula, sufficient in raw material source and rich in nutrition of raw material ingredients, the resource utilization rate is greatly improved, and the survival rate and weight gain rate of sepiella maindroni are remarkably improved.

The invention discloses a marking method for calcein of sepiella maindroni otolith and a detection method thereof. The marking method comprises the following steps: adding calcein into aquaculture water, thereby preparing into a solution in concentration of 200-400mg/L; exposing to the sun; adding mianserin and uniformly mixing, thereby acquiring a marked soaking solution; hungering the to-be-marked sepiella maindroni and then soaking in the marked soaking solution; and soaking in fresh seawater after ending the soaking in the marked soaking solution, thereby acquiring the mark of the sepiella maindroni otolith. The invention has the beneficial effects that the marking method disclosed by the invention is simple, the purpose of nondestructive marking can be achieved and the large-scale release marking action can be realized at one time; the mixed compound with high-strength fluorescent light can be formed according to the marking method, the result observation and comparison become convenient and the marking time is long-lasting; the marking method is suitable for the sepiella maindroni in different stages; and an effective method is supplied for evaluating the proliferation release effect of the sepiella maindroni.

The invention discloses a sepiella maindroni egg adhesive device. The sepiella maindroni egg adhesive device is convenient in egg taking operation, and the problem that in the ascending process of the egg adhesive device, adhesive eggs adhering to the egg adhesive device fall off and run off due to sea water shock can be effectively avoided. The sepiella maindroni egg adhesive device comprises a storage box which is sleeved with an annular air bag, an egg adhesive mechanism and a linkage air inflation and exhaust mechanism, wherein the egg adhesive mechanism comprises a plurality of vertical guide rails arranged in the storage box side by side, egg adhesive otter boards which correspond to the vertical guide rails one to one and can ascend and descend along the vertical guide rails, and a floating box which is located above the storage box and provided with a closed inner cavity; the egg adhesive otter boards are connected with the floating box through otter board connecting rods.

The invention relates to a releasing method for sepiella maindroni fries. The method includes the following steps that a, preparation work includes the steps that when a living water transport ship hold or a transport cask is used for loading and transporting the sepiella maindroni fries to a releasing sea area, a water inlet valve of the living water hold or transport cask is closed, water is drained to the depth of 20 cm-40 cm, a water drainage port valve is closed, and oxygenation is stopped; b, fry collecting includes the steps that a living water hold opening is covered with black-out cloth, and a light source with the illumination degree of 1000 lx-2000 lx is aligned with corners of the living water hold for illumination; c, siphon releasing includes the steps that a plastic pipe is used for siphoning the fries into sea water from the corners of the living water hold, wherein the plastic pipe is smooth in inner wall, and the diameter of the plastic pipe is larger than or equal to 6 cm; in the process of siphoning, the water inlet valve of the living water hold is opened, water inlet amount is adjusted to make the water depth of the living water hold be kept from 20 cm to 40 cm until the fries are released into the sea. The phenomenon that due to stress reaction, the sepiella maindroni fries perform ink squirt is reduced, and the survival rate of the released fries is increased. Meanwhile, the labor intensity for releasing is lowered, and the time for releasing is shortened, and efficiency is improved.

The invention discloses a bottom sinking type centralized collecting device using hole entering habit of sepiella maindroni, and aims to provide a bottom sinking type centralized collecting device which can catch the individual sepiella maindroni, does not affect normal growth or propagation of the sepiella maindroni, can float up automatically after catching the sepiella maindroni, and thus facilitates actual catching operation. The bottom sinking type centralized collecting device using hole entering habit of sepiella maindroni comprises a floating rack, wherein the floating rack is providedwith a floating rack block, a plurality of vertical supporting rods, a plurality of supporting legs for supporting the floating rack and a plurality of sepiella maindroni catching devices; the supporting legs are used for supporting the floating rack and are positioned below the floating rack; the sepiella maindroni catching devices are in one-to-one correspondence to the vertical supporting rods; and each sepiella maindroni catching device comprises a balancing weight, a sepiella maindroni catching device, a triggering mechanism, a connecting device and an unlocking mechanism.

The invention discloses a compound feed used for feeding sepiella maindroni de rochebrune, comprising the following materials based on weight: basal feed 35 to 65kg, white fish meal 10 to 50kg, microcrystalline cellulose 3 to 8kg, dextrin 5 to 30kg and biological extracted oil 3 to 6.5kg; wherein, the materials are mixed with water to obtain a compound feed with a water content of 10 to 30 percent. The compound feed used for feeding sepiella maindroni de rochebrune has the advantages that the compound feed can be used to solve the problem in frozen little fish feeding and provides a base for the large scale manual feed of sepiella maindroni de rochebrune; meanwhile, the pollution degree of feeding water body polluted by frozen little fish is reduced and the survival rate of the sepiella maindroni de rochebrune is increased by more than 8 percent, furthermore, the feeding cost is reduced and the benefit of manual feeding sepiella maindroni de rochebrune is increased.

The invention discloses a Speiella maindroni seedling delivery device. The purpose is to provide the Speiella maindroni seedling delivery device which has the advantages of convenient operation and high efficiency, and can effectively resolve the problem of the survival rate of seedlings caused by the facts that the transparent nylon bag is inconvenient to fix and the seedlings are easy to waggle during transport. The Speiella maindroni seedling delivery device comprises a transport vehicle, a mounting rack arranged on the transport vehicle, and a plurality of temporary seedling feeding devices arranged on the mounting rack. The mounting rack comprises a vertical guide rod and a supporting platform which is lifted and lowered along the vertical guide rod. The temporary seedling feeding device comprises a temporary feeding barrel arranged on an installation frame, a sealing cover body matched with a temporary feeding barrel, and a vertical connecting rod arranged in the middle of the upper end face of the sealing cover body. The supporting platform is provided with a hole of the connecting rod which is corresponding to the vertical connecting rod. The upper end of the vertical connecting rod passes through the hole of the connecting rod. The upper side of the vertical connecting rod is arranged on the outer side surface of the vertical connecting rod. A connecting rod limiting block is arranged on the outer side of the vertical connecting rod and above the supporting platform.

The invention discloses a method for discriminating male and female sepiella maindroni. The method comprises the following steps of measuring lengths of left peduncle 3, left peduncle 4, left tentacula and mantle of the sepiella maindroni requiring gender discriminating; calculating by the following discriminants, wherein for male, F1=223.42X3+165.85X4+7.951X5-108.06; for female, F2=151.48X3+92.71X4+19.19X5-60.03; in the discriminants, replacing X3 with the length of left peduncle 3/mantle, replacing X4 with the length of left peduncle 4/mantle, and replacing X5 with the length of left tentacula/mantle. The method has the advantages that by utilizing the discriminants, the discriminating accuracy is high, the discriminating is visual and accurate, the operation is easy and simple, and the injury to to-be-distinguished organisms is avoided; the result is accurate, the operation is simple, and the injury to organisms is avoided.

The invention, which belongs to the technical field of gene engineering, provides a metabolic biomarker in a metabolic biomarker in a senescence process of sepiella maindroni. The metabolic biomarkercomprises uric acid. The screening method of the metabolic biomarker includes the following steps: collecting serum samples of individuals at different reproductive stages; preprocessing the serum samples; carrying out liquid chromatography-mass spectrometry analysis on the serum samples successively; screening LC-MS data processing to obtain metabolic biomarkers; carrying out comparison and identification on the metabolic biomarkers; and constructing a metabolic pathway of the biomarkers and carrying out analysis. According to the invention, micro metabonomics analysis is carried out on the serum of sepiella maindroni at different reproductive stages by using the high performance liquid chromatography-tandem mass spectrometry technology and screening is carried out; the content of uric acid is greatly reduced after spawning by being compared with the content before spawning. The following formulas are used: the content before spawning/the content after spawning=2.16; VIP=1.5385. The provided metabolic biomarker is a potential metabolic biomarker.

The invention discloses a microsatellite marker for identifying sepiella maindroni pedigree and also discloses an application of the microsatellite marker in identifying sepiella maindroni pedigree. The invention has the beneficial effects that the microsatellite marker for identifying sepiella maindroni pedigree screened according to the invention is in large number of allele, is in high polymorphism and can be used for identifying the population genetic structure of sepiella maindroni, genetic and breeding evaluation, paternity test, and the like. The application of the microsatellite markerwith nucleotide sequence shown as SEQ ID No:1-19 in identifying sepiella maindroni pedigree can make up the limitation of fuzzy genetic background and few molecular markers in the present sepiella maindroni germplasm monitoring. The method is high in efficiency and short in identifying period.

The invention provides a change feature analysis method of liver mtDNA copy number in the sepiella maindroni aging process. The method includes: sample genome DNA extraction, single copy gene primer design, real-time quantitative PCR standard preparation and standard curve establishing of nuclear genome and mitochondrial genome single copy gene, detection of mitochondrial genome copy number in a sample and content calculation, wherein a sample DNA extraction method includes: adding sepiella maindroni tissue into polybutylene succinate powder and alkyl ether ammonium sulfate, grinding together,and performing extraction, organic solvent separation and precipitation to obtain sample DNA. By applying fluorescent quantitative PCR technology, accurate detection and quantification of mitochondria can be realized, and analyzing change of liver mtDNA copy number in the sepiella maindroni aging process is conducive to providing a solution for increasing spawning rate and providing new referencefor studying human aging.

The invention discloses terminal equipment for discriminating male and female sepiella maindroni. The terminal equipment comprises a terminal body, wherein the terminal body is provided with a camera and a screen; the terminal is provided with a program for taking a picture of the sepiella maindroni to be discriminated, and calculating the peduncle or tentacula end positions from the picture; by artificially selecting the left peduncle 3 end, the left peduncle 4 end, the left tentacula end and the tail end in a point way, and measuring the distance differences between the measuring base line and the left peduncle 3 end, the left peduncle 4 end, the left tentacula end and the tail end, the program can calculate the lengths of the left peduncle 3, the left peduncle 4, the left tentacula and mantle length; by adopting a discriminant to calculate, the type is judged according to the maximum values obtained by F1 and F2, wherein F1 represents male, and F2 represents female. The terminal equipment has the advantages that by utilizing the discriminant, the discriminating accuracy is high, the discriminating is visual and fast, the operation is easy and simple, and the injury to to-be-distinguished organisms is avoided, the result is accurate, the operation is simple, and the injury to organisms is avoided.