The invention discloses a tool plate jacking and positioning device which comprises a feeding carrier plate. A jacking module, a jacking blocking module and a non-return module are arranged on the feeding carrier plate, the jacking module comprises a jacking air cylinder and a jacking supporting plate, the jacking supporting plate is located in a jacking station, the jacking air cylinder drives the jacking supporting plate to ascend and descend, a tool bottom plate is supported on the jacking supporting plate and ascends and descends along the jacking supporting plate, the jacking blocking module comprises a jacking blocking air cylinder and a jacking blocking block, the jacking blocking air cylinder drives the jacking blocking block to do reciprocating motion, the jacking blocking block limits the front end of the tool bottom plate, the non-return module comprises a non-return elastic block doing elastic reset motion, and the non-return elastic block limits the rear end of the tool bottom plate. The structure is simple and reasonable, feeding operation is completed in a full-automatic mode, operation is convenient and fast, the labor intensity is greatly reduced, feeding work is stable and reliable, working efficiency is improved, feeding precision is effectively ensured, and safety coefficients are improved.