The utility model provides a kind of positioning mechanism and freight equipment, positioning mechanism is used to position guiding transport car, and guiding transport car is provided with cooperating piece, and positioning mechanism includes: installation main body, first avoiding opening is provided on installation main body;Baffle, it is set on installation main body, baffle has blocking position and avoiding position, baffle is rotatably arranged between blocking position and avoiding position;At least part of baffle is located in first avoiding opening, to form limiting space for limiting cooperating piece between installation main body, so that baffle is in blocking position, and guiding transport car is positioned by cooperating piece;At least part of baffle rotates to the upper side of first avoiding opening, to avoid cooperating piece, so that baffle is in avoiding position, and cooperating piece is separated from positioning mechanism by first avoiding opening.The present application solves the problem that the connection and disconnection operation between AGV dolly and goods shelf car in the prior art is inconvenient.