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Metabolic biomarker in metabolic biomarker in senescence process of sepiella maindroni

A technology of squid and biomarkers, which is applied in the field of metabolic biomarkers, can solve the problems of no squid and other problems, and achieve the effect of convenient operation and reasonable design

Pending Publication Date: 2019-12-31
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant research report on squid mansoni at home and abroad

Method used

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  • Metabolic biomarker in metabolic biomarker in senescence process of sepiella maindroni
  • Metabolic biomarker in metabolic biomarker in senescence process of sepiella maindroni
  • Metabolic biomarker in metabolic biomarker in senescence process of sepiella maindroni

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Experimental program
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Embodiment 1

[0032] A screening method for metabolic biomarkers in the aging process of squid mansoni

[0033] 1. Collect serum samples from individuals at different reproductive stages:

[0034] The squid used was obtained from the squid seed breeding and cultivation demonstration base in Shacheng Town, Fuding City, Fujian Province, and individuals at different reproductive stages were collected (before sexual maturity and spawning; during spawning; after spawning) When the serum samples were dying), they were marked as three groups A, B, and C, and each group had 6 replicates. In addition, in order to obtain high-throughput data with high sensitivity and high resolution, an equal mixture of all samples to be tested was set as a quality control group (QC group).

[0035] 2. Pretreatment of serum samples:

[0036] Take 100 μL of serum, add 3 times the volume of acetonitrile, vortex and shake for 1-3 minutes, let stand at 4°C for 10 minutes, centrifuge at 13000r / min for 10 minutes in a ce...

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Abstract

The invention, which belongs to the technical field of gene engineering, provides a metabolic biomarker in a metabolic biomarker in a senescence process of sepiella maindroni. The metabolic biomarkercomprises uric acid. The screening method of the metabolic biomarker includes the following steps: collecting serum samples of individuals at different reproductive stages; preprocessing the serum samples; carrying out liquid chromatography-mass spectrometry analysis on the serum samples successively; screening LC-MS data processing to obtain metabolic biomarkers; carrying out comparison and identification on the metabolic biomarkers; and constructing a metabolic pathway of the biomarkers and carrying out analysis. According to the invention, micro metabonomics analysis is carried out on the serum of sepiella maindroni at different reproductive stages by using the high performance liquid chromatography-tandem mass spectrometry technology and screening is carried out; the content of uric acid is greatly reduced after spawning by being compared with the content before spawning. The following formulas are used: the content before spawning / the content after spawning=2.16; VIP=1.5385. The provided metabolic biomarker is a potential metabolic biomarker.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a metabolic biomarker in the aging process of squid mansoni. Background technique [0002] Sepiella japonica belongs to the phylum Molluscs, Cephalopoda, Order Sepia, Sepiaceae, and the genus Sepia. It is an important economic species in the East China Sea and has high edible, medicinal and economic value. The life cycle of Squid mansonii is generally only one year, and its resources are composed of the supplementary groups of that year. However, since the mid-1970s, due to the deterioration of environmental conditions and overfishing by fishermen, the resources of squid mansoni in my country have been greatly damaged and even tended to be exhausted. In order to quickly restore the resources of squid mansoni in our country and carry out resource restoration work, it is important to increase the intensity of artificial breeding of squid mansoni, implement ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N30/02G01N30/06
CPCG01N33/5308G01N30/02G01N30/06G01N2800/7042
Inventor 郭宝英徐鸣祁鹏志许中天
Owner ZHEJIANG OCEAN UNIV
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