102results about How to "Shorten acclimatization time" patented technology

The invention discloses an automatic device for evaluating the memory function of a primate experimental animal and a method for evaluating the memory function of the primate experimental animal. The device comprises an experimental operation cage (1), a touch display screen (15), a horizontal exhibition stand (12), an infrared ray sensing automatic award food distributor (16) and a main control computer (19). Through interactive operation of the touch display screen and the animal, direct interactive operation between experiment personnel and the animal is avoided, and the evaluation of the cognitive ability is more objective. Through the device, the brain function can be evaluated subjectively according to a judgment made by a tester. The device and the method are particularly suitable for evaluating the cognitive memory of primate animals, particularly old non-human primate experimental animals and disease model non-human primate experimental animals.

The present invention relates to one kind of functional biological carbon fiber and its preparation process and application as biomembrane carrier. The biomembrane carrier is prepared with polyacrylonitrile carbon fiber, and through electrochemical surface treatment in 5-20 % concentration ammonium bicarbonate electrolyte to increase the surface acid oxygen containing functional groups and raise wettability, and gluing with water soluble epoxy resin in the amount 0.5-2 %. The biomembrane carrier can fix nitrobacteria, sulfate reducing bacteria and anaerobic methanogen. It may be used in treating high concentration acrylon waste water, and can eliminate sulfate, COD and ammonia nitrogen effectively. In addition, the biomembrane carrier is easy to regenerate and has no secondary pollution.

The invention provides a method for starting an SNAD process based on non-woven fabric ring packing and belongs to the technical field of water treatment. One packing is adopted to carry out a nitrosation reaction, an anaerobic ammonia oxidation and a denitrification reaction at the same time in one reactor. The non-woven fabric ring packing is composed of an inner layer non-woven fabric and an outer layer framework. Anaerobic ammonium oxidation bacteria can be bred on the surface of the packing through domestication and cultivation, partial nitrate is transformed into nitrogen through feeding organic carbon sources and domesticated heterotroph type denitrifying bacteria, finally, nitrosobacteria are fed, so the outer layer nitrosobacteria can consume most dissolved oxygen and transform partial ammonia nitrogen into the nitrite; accordingly, degradation substrates at a proper proportion can be provided for growth and metabolism of ANAMMOX bacteria, and meanwhile a beneficial anaerobic environment is provided for the denitrifying bacteria. The method has the advantages of being better in film culturing effect and shorter in domestication period and is a sustainable wastewater treatment technology, thereby being wide in application prospect.

The invention discloses a method for degrading ammonia nitrogen in high-salinity sewage by microbes, wherein a treatment system in the method comprises an anaerobic biochemical reaction tank, a first-stage aerobic biochemical pool and a second-stage aerobic biochemical pool. Firstly, the sewage and the recirculation water simultaneously flow into the anaerobic biochemical reaction tank to be treated by denitrification and hydrolysis reaction; then, the sewage orderly flows into the first-stage aerobic biochemical pool and the second-stage aerobic biochemical pool to be respectively treated by first-stage aerobic treatment and the second stage aerobic treatment; the active sludge in the first-stage aerobic biochemical pool is the domesticated salt-tolerant bacteria for performing first-stage nitration on the ammonia nitrogen, and besides the domesticated salt-tolerant bacteria, efficient nitrifying bacteria are also added into the second-stage aerobic biochemical pool for performing second-stage high-strength nitration on the ammonia nitrogen=, thus, the effluent ammonia nitrogen and the COD (Chemical Oxygen Demand) can reach the emission standard.

The invention relates to a method for preparing selective microbial packing. A plant organic fiber or a mixture of the plant organic fiber and activated carbon is used as basic packing, and the method comprises the following steps of: screening, separating and purifying activated sludge to obtain specific desulfurization bacteria; massively propagating and culturing the bacteria in nutrient solution to prepare desulfurization bacterium mother solution; and performing nutrient solution soaking, spraying and mother solution biofilm formation on the basic packing to prepare the selective microbial packing. The biological packing is used for microbial purification in waste gas treatment, and is particularly used for removing stinky pollutants produced in petrochemical plants, sewage treatment plants, urban sewage treatment plants, animal husbandry farms and the like. By the method, class I pollutants in exhaust gases of a refinery are treated specially, the treatment load and efficiency of the pollutants are improved, and impact resistance during change of working conditions is improved.

The invention relates to a method for relieving anaerobic ammonium oxidation system salinity shock with betaine as an additive. According to the invention, a successfully started anaerobic ammonium oxidation SBR reactor is used for processing salt-containing wastewater, wherein a volatile suspended sludge concentration is maintained at 6-9g / L, an inlet water NH4<+>-N concentration is 180-220mg / L, and the molar ratio of NH4<+>-N to NO2<->-N is controlled at 1:1-1:1.32 through adding (NH4) 2SO4 and NaNO2; the betaine concentration in the reactor is maintained at 0.1-5mmol / L through adding betaine; with a heating band, the temperature of the reactor is controlled at 30-35 DEG C; DO is controlled at 0-0.1mg / L; inlet water pH is stabilized at 7.0-8.5 through dropping NaOH / HCl; anoxic stirring is carried out for 4-8h after water feeding; when stirring is finished, the material is allowed to stand and to precipitate; when precipitation is finished, a supernatant is discharged. With the method provided by the invention, anaerobic ammonium oxidation activity recovery time when the anaerobic ammonium oxidation system is under salinity load shock can be effectively shortened, nitrogen removal rate can be improved, and a higher total nitrogen removal rate can be realized.

The invention provides a method for domestication of salt-resistant activated sludge. The method comprises that substances (such as soil or water) in a high-salt environment are directly added into activated sludge having a normal salt concentration according to a certain ratio and then salt concentration loading is gradually increased, and in the above process, the microflora in the high-salt environment and the microflora in the activated sludge carry out bidirectional coevolution so that novel microflora is formed. The method is free of a complex bacterium screening process, has simple and easy processes, utilizes a novel salinity gradient-increasing scheme, prevents a biomass loss caused by overquick increasing of salinity pressure and realizes fast and efficient domestication of high-salt sludge.

The invention relates to mobile skid-mounted equipment for efficient water treatment. The mobile skid-mounted equipment for efficient water treatment can operate continuously or intermittently and is characterized by comprising three independent skid-mounted parts, namely, a pre-treatment flocculent precipitation system, a biological treatment system and a deep membrane treatment system, and the integrated mobile skid-mounted treatment equipment is communicated with a sewage tank through a conveying water pump; in terms of flocculent precipitation, a specific agent is adopted, and suspended matter as well as poisonous and harmful heavy metal and the like can be removed through accurate flocculation, stirring and precipitation; the biological treatment system comprises an aerobic tank, an MBR (membrane biological reactor) tank and the like; the deep treatment device can remove high salt and other ions through filtration of reverse osmosis membranes. The equipment has the benefits as follows: discharged water meets the national discharge or reuse standard after collected waste water in oil and gas fields is treated through the integrated skid-mounted equipment for water treatment. The equipment has the advantages of advanced technique, stable performance, good treatment effect, little investment, simplicity in operation, convenience in management, use and maintenance and the like and is a whole set of mobile treatment device specially designed according to water quality characteristics of the waste water in the oil and gas fields.

The invention discloses a method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using an aerobic dynamic drainage method and relates to a method for preparing the PHA. The invention solves the problems that the acclimatization time for synthesizing PHA through active sludge mixed flora is long, a reactor works unstably and the synthesized PHA is low in content in the prior art. The method comprises the following steps: 1, active sludge is put into a sequencing batch reactor (SBR); 2, 6-12 hours are taken as a cycle of operation, in the cycle of operation, two-time water introduction, precipitation and drainage are carried out, the drainage rate is 1:1, during the first water introduction, a carbon source and various nutrients are introduced, and during the second water introduction, only the supplement of nitrogen nutrient is carried out; 3, 2-4 cycles are operated every day, the sludge detention time is 1-10 days and sludge-water mixed liquid is collected; 4, batch experiments are carried out, half supernatant is discharged, equivalent carbon source is supplemented, aeration, precipitation and drainage are carried out, and the process is repeated for 6 to 8 times. The invention belongs to the field of biodegradable plastic synthesis.

The present invention discloses a method for producing the compound micro-organism microbial inoculum that is used for degrading the waste gas of VOCs. Firstly, culture medium containing the triphenyl substance is adopted to naturalize the active mud that is taken from a sewage treatment plant; the active dirt that is obtained in the domestication way and has high degrading performance is used as the mixed strain to be inoculated to the microbial inoculum carrying material, and the compound micro-organism microbial inoculum can be obtained after the solid fermentation culture. The preparing method comprises the steps of domestication of the active mud, mixing of the microbial inoculum carrying material at a giving ratio and the solid fermentation culture, so that the preparing method is simple, and the cost is low, and the advantages that the species group of the active mud mixture micro-organism is rich, the bacteria group structure is reasonable, and the microbial inoculum favors the long-term storage are effectively combined. Since the preparing method is used for preparing the target pollutant, the domestication time in the actual application of the waste-gas organism treatment can be shortened, and the rapid startup of the reaction device can be accelerated. The active mud rapid-startup waste-gas organism purification reactor can be substituted, so as to realize the high-efficient degrading of the target pollutant.

The invention relates to application of functional biological carbon fiber. The carbon fiber of which the surface contact angle is 50 to 180 degrees and the range of equilibrium moisture content is 1.0 to 5.9 percent is adopted as a biofilm carrier. The biofilm carrier can rapidly fix nitrobacteria, denitrifying bacteria, sulfatereducting bacteria and anaerobic methanogens. Acrylic and other high-concentration production wastewater is treated mainly through two-phase anaerobic / aerobic nitration and other processes, so that sulfate, COD, BOD, ammonia nitrogen and nitrate nitrogen can be effectively reduced and removed. In addition, a regeneration method of the biofilm carrier is simple and easy to apply and free from secondary pollution. The invention is particularly applicable to the biological treatment of acrylic wastewater and other industrial wastewater with high sulfate, high COD, low carbon-sulfur ratio and substances difficult to biodegrade.

The invention discloses an anaerobic fluidized bed series constructed wetland type microbial fuel cell device and method. The anaerobic fluidized bed series constructed wetland type microbial fuel cell device comprises an anaerobic fluidized bed and a constructed wetland type microbial fuel cell which are connected in series; the anaerobic fluidized bed cultures a carrier to which a biofilm is attached as an anode of the constructed wetland type microbial fuel cell. Compared with a traditional active sludge method, a novel sewage treatment system of the anaerobic fluidized bed and the constructed wetland type microbial fuel cell, provided by the scheme, has the advantages that two devices are connected in series to realize deep treatment of sewage and improve the treatment effect of waterquality; the anaerobic fluidized bed can culture the carrier to which the biofilm is attached, and the carrier is used for the anode of the constructed wetland type microbial fuel cell, so that startof the microbial fuel cell can be accelerated.

The invention discloses a method for quickly starting a pre-denitrification biological aerated filter process, belongs to the field of sewage treatment, and solves the problem that the conventional pre-denitrification biological aerated filter process consumes long time to culture pre-denitrification flora so as to result in over-long starting time. The method for quickly starting the pre-denitrification biological aerated filter process adopts the following mode: firstly, forming biofilm; secondly, emphasizing on culturing naturalized nitrobacterium; and finally culturing naturalized denitrified bacteria. Therefore, the acclimation link is saved, the acclimation time is shortened, and the debugging cost is reduced. The method can rapidly culture the denitrified bacteria in an anoxic denitrification biological aerated filter in the pre-denitrification biological aerated filter process and finish the debugging for putting into use as soon as possible. Due to the adoption of the method, the effluent of the pre-denitrification biological aerated filter process can be debugged to reach the standard in 120 to 140 days.

The invention discloses a serum-free medium for full-suspension culture of MDCK cells (madin-darby canine kidney cells) and a preparation method of the serum-free medium. The serum-free medium for full-suspension culture of the MDCK cells consists of a basic metabolism nutrient, nucleotide, vitamins, inorganic salt, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives; and the preparation method of the serum-free medium for full-suspension culture of the MDCK cells comprises the following steps: 1) preparing mixed liquid: dissolving and mixing the raw materials; and 2) regulating pH: regulating pH of the mixed liquid to 6.3-6.7, and setting a constant volume, so that the serum-free medium for full-suspension culture of the MDCK cells is obtained. The medium supports the high-density full-suspension culture of the MDCK single cells and is capable of greatly shortening a time of domesticating the MDCK cells from adherent cells into the serum-free full-suspension cells; and the medium is applicable to the large-scale production of biological products, in particular veterinary biological products.

The invention discloses a biological product containing aerobe combined bacteria and a preparation method and application thereof. The biological product includes aerobe combined bacteria loaded on a porous carrier; and the aerobe combined bacteria includes bacillus and pseudomonas putida. The preparation method includes the steps of: 1) soaking the porous carrier with acid or alkali and rinsing the porous carrier with water to a neutral state to obtain pretreated porous carrier; and 2) loading the aerobe combined bacteria liquid on the porous carrier. The biological product can be used in sewage treatment, and significantly improve the efficiency of biochemical treatment, efficiently remove COD and ammonia nitrogen, improve the outlet water quality and reduced the sludge yield by more than 80%.

The invention discloses a method for removing organic arsenic in livestock cultivation waste water by utilizing three-stage anaerobic treatment process. The method comprises the steps of adding pulverized fuel ash and slaked lime mixture between an acid producing phase and a methane producing phase in a two-phase anaerobic treatment as an adsorbed phase of an adsorbing medium, and forming the three-stage anaerobic treatment process for removing the organic arsenic in the livestock cultivation waste water. The treatment process has good removal effect on the organic arsenic, and has arsenic content in treated water to be lower than 20mug / L, which is lower than the emission standard of waste water in China, thus realizing treatment of wastes with processes of wastes against one another, reducing harm of arsenic to the environment and human health by utilizing the pulverized fuel ash and the slaked lime to adsorb and fix the organic arsenic, and fully utilizing solid wastes as the treated pulverized fuel ash and the slaked lime can be used as building materials. Simultaneously, the activity of methane producing bacteria can be improved, and the running stability of a reactor can be enhanced.

A preparation method of a halophilic decontamination bacterial agent and the bacterial agent prepared by the same belong to the technical field of biological treatment of salinity wastewater. The preparation method comprises the following steps: 1) inoculating the test tube strain of the halophilic decontamination bacterium into nutrient agar for culturing; 2) inoculating the cultured strain into a fermentor medium of preset volumetric quantity according to the inoculation quantity which is 1-2% of the medium weight to be cultured till logarithmic growth phase; and 3) in the fermentor production process, maintaining the ventilation volume of aseptic air to be 4.0-6.0L / min, the stirring rate to be 60-120r / min, the culture temperature to be 25-40 DEG C and the full-process culture time to be 24-48h, subpackaging the culture solution after fermentation or adopting aseptic attachments for adsorption and drying, thus preparing the solid bacterial agent. The strain of the bacterial agent was collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.3314. The invention adopts the bioaugmentation technology, greatly improves the capability of treating salinity wastewater and has low cost and significant effects.

The invention discloses a plant yeast additive and a preparation method thereof and relates to the technical field of feed additives. The components of the plant yeast additive comprise the following raw materials in percentage by weight: 2-2.5% of dry carambola leaves, 3-3.5% of dry sugarcane leaves, 3-3.5% of dry granadilla leaves, 2-2.5% of dry wild chrysanthemum flowers, 2-2.5% of brown sugar, 0.1-0.15% of dry yeast and the balance of water. The preparation method comprises the following steps: placing the dry carambola leaves, the dry sugarcane leaves, the dry granadilla leaves and the dry wild chrysanthemum flowers into boiling water and soaking for 2-3 hours; then, filtering residues and impurities of the carambola leaves, the sugarcane leaves, the granadilla leaves and the chrysanthemum flowers; and after standing the filtrate, adding the dry yeast and the brown sugar and fermenting for at least 30 days to form sourness to obtain the plant yeast additive. According to the preparation method disclosed by the invention, a fermentation solution obtained after the soak solutions of the four plants are fermented is used as the plant yeast additive, so that not only can the treating effect be improved, but also the sludge domestication time can also be shortened.

The invention discloses a method for enhancing high-concentration ethylbenzene waste gas treatment by using a biological trickling filter. According to the invention, a biological trickling filter device is inoculated by using active sludge; domestication is carried out under low-concentration ethylbenzene waste gas; when domestication is finished, the ethylbenzene is regulated to a high concentration range; when high-concentration ethylbenzene removing efficiency is stabilized, a non-ionic surfactant and an inorganic salt cosolvent are added into a nutrient solution; with a solubilization effect of the surfactant, a mass transfer process of slightly soluble organics between three phases of gas, liquid, and bio-membrane is promoted. Therefore, bioavailability is improved, and degradation efficiency is improved. With the method provided by the invention, running device and other running conditions are not changed, and operation process continuous long-time running can be ensured. The method is economical, and has the advantages of high efficiency, environment friendliness, and convenient operation and maintenance.

The invention discloses an efficient microbial nutrition enhancer which comprises the following components by weight percent: 3-8% of K, less than or equal to 0.1% of Co, less than or equal to 0.1% of Mo, less than or equal to 0.1% of Ni, less than or equal to 0.05% of Ga, less than or equal to 0.08% of Im, 0.5-2.0% of butyric acid, 2-6% of synergist and the balance of water. According to the efficient microbial nutrition enhancer, the synergist is introduced into the enhancer, so that the time used for cultivating and acclimating anaerobic activated sludge in the anaerobic biochemical treatment can be shortened, the stability of the whole anaerobic biological treatment process is improved, and the total quantity of probiotics in the acclimated sludge can be greatly increased.

The invention discloses a sludge acclimation method for acclimatizing bacteria in active sludge to high salty environment. The method includes that (1) the salinity of the industrial waste water is measured; (2) an array with a last term of 1 is selected according to the estimated acclimation period and the salinity of the objective sewage, and the salinity of each prepared water can be obtained by the salinity of the waste water multiplying each item of the array with the last term of 1; (3) the mixed sewage is prepared according to the calculated salinity of the prepared water for the acclimation of the active sludge. The method of the invention has the advantages of simple and easy technique and strong operability, improves the success rate of the sludge acclimation and the treatment effect of the sludge, shortens the sludge acclimation period, fastens the acclimation speed of the active sludge in the reactor and saves the cost.

The invention discloses a biological oxidized filter material, a method for preparing the biological oxidized filter material and an application thereof to odor treatment. The biological oxidized filter material comprises the raw materials in weight percentage: 50 to 70 percent of dairy manure, 5 to10 percent of poultry dung, 6 to 10 percent of corn straw, 1 to 3 percent of corn flour, 1 to 4 percent of straw, 12 to 15 percent of soil and 5 to 8 percent of mud. The filter material is characterized by fast desired effect, extensive and secure odor treatment, long service life, etc.

The invention discloses an immobilized microalgae reactor taking mycelium pellets as the carriers and a wastewater processing method. The reactor comprises a water inlet system, a reactor body, and awater outlet system. The water inlet system comprises a water inlet box. The water inlet box is sequentially connected to a water pump and a water inlet pipe. The reactor body comprises an uprising pipe and a descending pipe. The other end of the water inlet pipe is communicated with the top end of the descending pipe. The uprising pipe is arranged in the cavity of the descending pipe. The bottomof the descending pipe is provided with an aeration head. The aeration head is communicated with an air compressor through an air inlet pipe. The water outlet system comprises a water outlet box and awater outlet pipe. The water outlet pipe is installed on the lateral wall of the descending pipe. The water outlet pipe is communicated with the descending pipe. Water from the water outlet pipe is introduced into the water outlet box. The wastewater processing method comprises following steps: manufacturing a reactor, preparing a condensed alga solution, mycelium pellet suspension, flocculant fermentation broth, and photosynthetic bacteria concentrate, and rapidly starting and running a bacterium-alga commensalism system. The problems that the time for generating a mycelium pellet-microalgaeparticle system is long and the stability of the system is bad are solved.

The invention discloses a preparation method of a denitrification embedding immobilized granule. The preparation method includes the steps of: a) dissolving polyvinyl alcohol in water at high temperature and successively adding alginic acid and SiO2 to fully dissolve the raw materials to obtain a PVA-SA hydrogel solution, and cooling the solution; b) mixing a nitrobacteria microbial agent with thePVA-SA and the hydrogel solution, and fully stirring the components to uniformly mix the microorganisms and the hydrogel; c) dropwise adding the mixture to a crosslinking agent prepared from boric acid and aluminum sulfate, immobilizing the components, and washing the product with clear water and drying the product. In the invention, with the powdery nitrobacteria microbial agent as a target microbial agent, the denitrification embedding immobilized granule is produced. Compared with a method of enriching nitrobacteria, difficulty of mechanical manufacturing is greatly reduced; the method promotes the development of industrial wastewater treatment of embedding immobilization.

The invention discloses a domestication method and application of a nuclear polyhedrosis virus adapted to spodoptera frugiperda. The domestication method includes the following steps: performing UV irradiation on an autographa californica nuclear polyhedrosis virus, applying the treated virus to artificial feed of the spodoptera frugiperda to obtain virus-containing feed, feeding hungry spodopterafrugiperda larvae with the virus-containing feed, collecting poisonous insects or dead insects, performing crushing, performing filtration, and performing centrifugal collection to obtain a free-state autographa californica nuclear polyhedrosis virus; and performing iteration culture and amplification alternately on the free-state autographa californica nuclear polyhedrosis virus by using isolated cells and insects of the spodoptera frugiperda to obtain a domesticated autographa californica nuclear polyhedrosis virus, and improving viral titer of the domesticated virus to the spodoptera frugiperda by an insect cultivating method to obtain the nuclear polyhedrosis virus adapted to the spodoptera frugiperda. The autographa californica nuclear polyhedrosis virus provided by the invention hasthe characteristics of strong pertinence, long control acting time, no side effects, and difficulty in producing drug resistance by pests, and the virus domestication efficiency is fast and efficient.

The invention discloses a high-efficiency biological composite carbon source, and the biological composite carbon source comprises the following active ingredients by mass content: 20.0-28.0% of glucose and 35.0-45.0% of crude glycerin; and the balance is water, and the sum of mass content is 100%. The high-efficiency biological composite carbon source can effectively provide the sufficient carbonsource for the biological flora in a biochemical system, and can ensure the safety of a use process.

The present invention discloses a method of culturing and storing integrated nitrobacteria and a reaction vessel. The distribution device of the reaction vessel is placed inside a tank body. An air inlet tube and an air outlet tube are respectively extended inside the tank body to be connected with the distribution device. The position of the distribution device inside the tank body can be adjusted up and down by the air inlet tube and the air outlet tube. The culturing step and the storing step are processed inside the same reaction vessel with the air inlet tube, the air outlet tube and an air distribution device. The culturing step is that the air distribution device is placed at the bottom of a vessel; the temperature is kept between 20 DEG C and 35 DEG C; culture medium dissolved oxygen is higher than 2.0mg / L. The storing step is that the temperature is kept between 20 DEG C and 35 DEG C; the air distribution device is adjusted to be higher than one third distance from the bottom; the culture medium dissolved oxygen is less than 0.8mg / L; the glycerin or glucose of 1.8 to 3.0g / L is added according to the cubage of the culture medium. The present invention ensures that the half-life of the nitrobacteria reaches 170 days to 220 days, reduces the production steps, improves the working efficiency and provides technical support for the large scale production of the nitrobacteria.

The invention discloses a modified filling for a waste gas bio-trickling filter and a preparation method of the modified filling. The preparation method comprises the steps of after washing ceramic particles with the particle sizes of 5mm-100mm with water, steeping the ceramic particles in hydrochloric acid, taking out the ceramic particles, washing the ceramic particles with water, steeping the ceramic particles in an NaOH solution, taking out the ceramic particles, washing the ceramic particles to be neutral with water, drying, steeping with a modifier, draining off, and drying, so as to obtain the modified filling for the waste gas bio-trickling filter. The modified filling for the waste gas bio-trickling filter and the preparation method are particularly applicable to bio-trickling filter devices, the absorption effect of the obtained modified filling to degrading bacteria is good, the waste gas treatment efficiency of the bio-trickling filter can be effectively improved, meanwhile, the raw material cost is low, the processing process is simple, and the market application prospect is wide.

The invention discloses a modular microorganism in-situ domestication method adaptive to a fast water flow, belonging to the fields of designs in environmental engineering, ecological engineering and hydraulic engineering. The modular microorganism in-situ domestication method adaptive to the fast water flow mainly comprises the following steps: (1) flow guide dike arrangement, (2) water inlet weir construction, (3) aeration device arrangement, (4) microorganism adhesion substance module construction and fixation, and (5) microorganism preparation throwing. According to the method, domestication areas are delimited, water flow gradient and dissolved oxygen gradient are changed, and advanced in-situ domestication is performed on river microorganisms step by step, so that then adaptation time of the microorganisms is reduced when the microorganisms are transplanted to the same riverway for ecological restoration, the survival rate of the microorganisms is increased favorably, and a microorganism purification effect is enhanced.

The invention relates to a circulation type methane matrix membrane biological membrane reactor and a sewage treatment method. A seal head seals the upper ends of a main reaction region and is provided with an air inlet pipe, one end of a hollow fiber membrane is sealed in the seal head and communicated with the air inlet pipe, the lower portion of the main reaction region is connected with an anti-impact porous plate, the lower portion of the anti-impact porous plate is connected with a sludge region, a blow-down pipe is arranged at the bottom of the sludge region, the main reaction region and a circulating pipe are connected together to from an upper transiting pipe and a lower transiting pipe, the upper end of the circulating pipe is connected with a driving motor, a motor shaft of thedriving motor is connected with an axial-flow type stirring paddle, a water inlet pipe is arranged at the lower end of the circulating pipe, and a water outlet pipe is arranged on one side of the upper end of the circulating pipe. The circulation type methane matrix membrane biological membrane reactor is convenient to operate; the effect of methane mass transfer is good, and the utilization rateis high; sludge easily attaches to the circulation type methane matrix membrane biological membrane reactor; nitrogen-containing wastewater can be treated.