Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same

Abstract

A preparation method of a halophilic decontamination bacterial agent and the bacterial agent prepared by the same belong to the technical field of biological treatment of salinity wastewater. The preparation method comprises the following steps: 1) inoculating the test tube strain of the halophilic decontamination bacterium into nutrient agar for culturing; 2) inoculating the cultured strain into a fermentor medium of preset volumetric quantity according to the inoculation quantity which is 1-2% of the medium weight to be cultured till logarithmic growth phase; and 3) in the fermentor production process, maintaining the ventilation volume of aseptic air to be 4.0-6.0L / min, the stirring rate to be 60-120r / min, the culture temperature to be 25-40 DEG C and the full-process culture time to be 24-48h, subpackaging the culture solution after fermentation or adopting aseptic attachments for adsorption and drying, thus preparing the solid bacterial agent. The strain of the bacterial agent was collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.3314. The invention adopts the bioaugmentation technology, greatly improves the capability of treating salinity wastewater and has low cost and significant effects.

Description

technical field The invention belongs to the technical field of biological treatment of salty wastewater, and in particular relates to a preparation method of a salt-tolerant decontamination bacterial agent and the bacterial agent. Background technique At present, many conventional physical, chemical and biochemical methods can be applied to the treatment of saline wastewater. There are large differences in treatment, especially the impact of high salinity, which increases the difficulty of sewage treatment. It has been found through research that the toxic effect of high concentration inorganic salts on wastewater biological treatment is mainly through the destruction of microbial cell membranes and enzymes in bacteria through elevated environmental osmotic pressure, thereby destroying the physiological activities of microorganisms. In addition, the salinity in the wastewater increases the density of the wastewater, resulting in a decrease in the specific gravity differen...

AI Technical Summary

Problems solved by technology

Known technologies cannot solve the problem of purification and treatment of saline wastewater, while bioaugmentation treatment technology has the characteristics of high efficiency, stability, and investment saving. Therefore, the development of high-efficiency bacteria and bacteria agents is the expectation of the industry, and the market urgently needs a microbial salt-tolerant cleaner. Biofouling agent developed to provide new way to purify seawater

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