10results about How to "The separation method is simple" patented technology

The invention relates to the technical field of biological detection, and discloses an instant staining lipoprotein sample separation method, which comprises the following steps: S1, lipoprotein staining: taking 10 [mu] L of a sample loading staining solution, adding 50 [mu] L of a plasma lipoprotein sample, and completing staining without waiting and heating incubation; s2, sample loading of dyed lipoprotein: assembling a sample density separation liquid pre-loading column in a disc electrophoresis tank, adding an electrophoresis buffer solution, and adding the lipoprotein sample dyed in the step S1 to the upper end of the sample density separation liquid pre-loading column; and S3, electrophoretic separation of the lipoprotein subtypes: switching on a power supply of an electrophoresis apparatus, enabling the lipoprotein with negative charges to migrate to a positive electrode under the action of an electric field, and realizing separation according to different particle sizes of the lipoprotein subtypes. The lipoprotein loading staining solution is good in dispersity and uniformity, and in the preparation process of the lipoprotein loading staining solution, the dye does not need to be filtered to remove insoluble substances and does not need to be heated; the staining solution is good in stability and can be stored at normal temperature.

The invention relates to the technical field of chemical synthesis, in particular to a preparation process of clindamycin phosphate, which comprises the following preparation steps: chlorination reaction, alcoholization reaction, ketonization reaction and esterification reaction. Adding 50-55 parts by weight of phosphorus oxychloride, 340-380 parts by weight of a chelating agent and 60-70 parts by weight of triethylamine, and stirring until the materials are dissolved; the preparation method comprises the following steps: adding acetone into clindamycin propylidene until the acetone is completely dissolved, then adding the acetone into the clindamycin propylidene, keeping the temperature at 5-15 DEG C to react for 4-8 hours, and then hydrolyzing, removing a chelating agent, decolorizing, adsorbing, desorbing and crystallizing to obtain a clindamycin phosphate finished product, the method has the advantages of reducing emission of pyridine hydrochloride and reducing environmental pollution.

This invention discloses a method and its application for isolating mitochondria from mesenchymal stem cells (MSCs) to improve their yield, purity, and activity. Compared to existing methods for isolating MSCs, the method of this invention effectively improves the yield and purity of MSCs, increasing the number of mitochondria by 2.96 times. The method also better maintains the activity of MSC mitochondria, significantly increasing cytochrome C content and exhibiting superior activity in treating autoimmune hepatitis. Furthermore, the method is simple, requires minimal reagents, and has broad application prospects in the field of mitochondrial transplantation therapy.

The application relates to the technical field of chemical synthesis, in particular to a heterogeneous catalyst which can be used for synthesizing benzyl phenylpropynoate compounds. x Ni y M z , M is at least one of Pt, Pd, Rh, Ru and Ir, x, y and z represent molar numbers, x+y+z=10, z is 1-2, and x:y is 1-8:1; and the carrier is a metal oxide or a molecular sieve. The heterogeneous catalyst can be used for synthesizing benzyl phenylpropynoate compounds by adopting a one-pot method, has high catalyst activity, good substrate universality, good stability, can be repeatedly used for more than 5 times, and can be separated by adopting a filtering mode, so that the separation method is simple. Moreover, a product post-treatment method is simple, and the target product has high yield.

The invention discloses a near-infrared fluorescent probe with an aggregation-induced emission enhancement effect. The near-infrared fluorescent probe is used for dual detection of hydrogen sulfide and hydrazine. The fluorescent probe is synthesized by carrying out Knoevenagel condensation reaction on 4-hydroxy-3-(1, 4, 5-triphenyl-1H-imidazolyl) benzaldehyde (I) and 2-(2-methyl-4H-chromene-4-subunit) malononitrile (II) under the catalytic action of piperidine, and the chemical structural formula of the fluorescent probe is as shown in the formula (III). The fluorescent probe shows high selectivity and high sensitivity to both hydrogen sulfide and hydrazine, the detection limits are as low as 50.1 nM and 89.2 nM respectively, and the fluorescent probe can be used for various scenes such as food spoilage monitoring and environmental water sample detection, can be used for biological imaging, and shows important application potential in the aspect of detection of hydrazine and hydrogen sulfide in organisms.

The invention provides a method for separating biflavone compounds from ferrugina flower buds, which specifically comprises the following steps: firstly, taking ferrugina flower bud powder, adding 95% ethanol for reflux extraction, and filtering to obtain filtrate; the preparation method comprises the following steps: firstly, filtering the raw materials to obtain a filtrate, then purifying the filtrate through semi-preparative HPLC (High Performance Liquid Chromatography) to obtain an eluent, detecting the eluent at 300nm, determining the eluent with the collection and retention time of 20.5-23.0 minutes and 38.4-40.6 minutes, and carrying out vacuum concentration and evaporation to dryness to obtain the biflavone compounds Rhusflavanone and Rhusflavanone. The separation method disclosed by the invention is simple, rapid, efficient and environment-friendly, and the obtained biflavone compound is high in purity. The biflavone compound obtained by the invention can be used as a reference substance, is used for measuring the content of the biflavone compound in the fermentum and counterfeit siamensis siamensis and siamensis siamensis and siamensis siamensis and siamensis siamensis, and effectively identifying the authenticity of a fermentum medicinal material or whether counterfeit replacement exists in a preparation or not, so that the technical blank is filled.

The invention discloses an efficient separation system for three-phase components of oily sludge and a separation method of the efficient separation system. The system comprises a homogenizing and tempering tank for dilution and viscosity reduction, a demulsification unit for chemical agent demulsification and thermal vibration agglomeration demulsification, a separation unit for three-phase separation and a recovery unit for recovering oil liquid, wastewater and oil sludge waste residues, an oil-containing sludge inlet of the homogenizing and conditioning tank is communicated with an oil-containing sludge outlet of the oil-containing sludge storage tank, a water inlet of the homogenizing and conditioning tank is communicated with a water outlet of the wastewater heating tank, and an oil-containing sludge outlet of the homogenizing and conditioning tank is communicated with an oil-containing sludge inlet of the demulsification unit; an oil-containing sludge outlet of the demulsification unit is communicated with an oil-containing sludge inlet of the separation unit; an oil phase outlet, a water phase outlet and an oil sludge solid phase outlet of the separation unit are respectively communicated with an oil phase inlet, a water phase inlet and an oil sludge solid phase inlet of the recovery unit; the oil-containing sludge is destabilized in a multi-stage enhanced synergistic demulsification mode, and efficient separation of three-phase components of the oil-containing sludge is realized through solid-liquid separation and oil-water separation.

The invention discloses a method for separating p-benzoquinone in phenol electrolytic circulating liquid, which comprises the following steps: carrying out centrifugal treatment on the phenol electrolytic circulating liquid, and taking supernate; dropwise adding organic alcohol into the supernate to obtain an organic phase; concentrating and drying the organic phase to obtain p-benzoquinone; wherein the volume ratio of the organic alcohol to the supernate is (0.5-5): 1. The organic alcohol compound is used as the separation solvent, the separation method is simple, the raw materials are wide in source and reasonable in price, and the prepared p-benzoquinone is high in yield, few in impurity and high in purity.

The invention discloses a heterogeneous catalyst, a preparation method and application of the heterogeneous catalyst in synthesis of carbonic ester compounds. The heterogeneous catalyst provided by the invention is MO-Cu-CMNDI, MO represents one of metal oxides such as MgO, Bi2O3, ZnO, CuO, Fe2O3, ZrO2, Ga2O3, CeO2, SiO2, Al2O3 and TiO2, preferably CeO2, and Cu-CMNDI represents a metal coordination network material [Cu (CMNDI) (H2O) 4] synthesized by CuSO4 and N, N '-bis (carboxyl methyl)-1, 4, 5, 8-naphthalimide (CMNDI). When the MO-Cu-CMNDI catalyst is used for the synthetic reaction of the carbonic ester compound, the catalytic activity is high, the reusability is good, and the yield of the reaction is improved.

This invention provides a method for preparing 2,5-furandicarboxylic acid in an acidic system, belonging to the field of bio-based chemical preparation. It is a method for synthesizing 2,5-furandicarboxylic acid by catalytic oxidation of 2,5-diformylfuran in an acidic environment. The method uses 2,5-diformylfuran as a raw material and employs an aqueous / organic two-phase catalytic system, using hydrogen peroxide to oxidize 2,5-diformylfuran to prepare 2,5-furandicarboxylic acid. In this invention, the raw material 2,5-diformylfuran and the catalyst are dissolved in the organic phase, the aqueous phase is acidic, and the oxidation product 2,5-furandicarboxylic acid precipitates in the aqueous phase. High-purity 2,5-furandicarboxylic acid can be obtained by simple filtration. The catalyst in the organic phase can be recycled multiple times. This invention does not use precious metal catalysts, does not require the addition of additional inorganic bases before or after the reaction, has simple product separation, generates little waste, and has broad application prospects.