402results about How to "The separation method is simple" patented technology

The invention discloses a hermetia illucens breeding system and belongs to the technical field of green cultivation. The hermetia illucens breeding system comprises a culture pond and a ceiling arranged at the upper part of the culture pond; the culture pond is sequentially provided with a water absorption layer, a heating device and a grid layer from bottom to top; the heating device comprises a heating tape and a fixing frame for fixing the heating tape; a compost is put on the upper part of the water absorption layer; the heating device is located in the compost; the grid layer is located on a surface layer of the compost; a lifting device is arranged on the side of the culture pond; the lifting device drives the heating device to lift; and a heat preservation cover is arranged at the top of the culture pond. Hermetia illucens is bred by using the hermetia illucens breeding system, so that the survival rate of hermetia illucens larvae is high and the hermetia illucens is not affected by an external environment; furthermore, the hermetia illucens is easily separated from the compost and insect feces do not need to be cleaned, so that the cost of manpower and material resources is greatly reduced. In addition, the compost in the culture pond is digested and fermented in the hermetia illucens breeding process and can be directly returned to the field as an organic fertilizer, so that the hermetia illucens breeding system is economical and environmentally friendly.

The invention relates to a separation method and a culture method for umbilical cord mesenchymal stem cells. The separation method comprises the following steps: thoroughly cleaning umbilical cord tissue of a healthy newborn by using a PBS (phosphate buffer solution) containing streptomycin and penicillin, and removing blood; shearing the umbilical cord into small sections uniform in length, and mechanically separating, bluntly stripping Wharton' s jelly, and removing umbilical arteries and umbilical veins; uniformly shearing the Wharton' s jelly; re-suspending the sheared Wharton' s jelly through an MSCs (mesenchymal stem cells) culture medium, inoculating to a culture dish with laid gelatin, and putting in a CO2 culture box for cultivation; conducting centrifugal separation to obtain tissue blocks and a cell resuspension solution. The culture method comprises the following steps: enwrapping the culture dish, discarding the gelatin, and washing with the PBS; inoculating the separated out tissue blocks and the cell resuspension into the culture dish; performing digestive subculture after cell fusion growth rate reaches 80-90%.

The invention discloses a noise separation-based power transformer noise assessment method. The steps include: 1) building a voiceprint feature database in advance according to voiceprint feature information corresponding to noise signals generated by a fan component, an iron core component and a winding component when a standard power transformer is in different running states; 2) collecting in real time sound wave signals sent when a transformer to be tested runs, so as to obtain noise signals to be assessed; 3) separating noise signals generated by the fan component, the iron core component and the winding component from the noise signals to be assessed; and 4) extracting voiceprint feature information of each noise signal, identifying the running state of the transformer to be tested, and assessing the noise level of each component according to the running state. The noise separation-based power transformer noise assessment method provided by the invention can realize assessment of the noise level of each component in the power transformer utilizing acoustic characteristics, and has the advantages of easy operation implementation and high assessment precision and efficiency.

The method relates to the method to extract the sunflower oil and recover the protein by water-enzyme method. The producing procedure comprises the following steps: dry-pulverizing the uncoated sunflower seed, adding citrate buffer to the powder and decomposing the powder by compound cellulose; centrifugalizing to get free oil, emulsion, digest and tablet; high speed centrifugalizing to get some free oil; filtering, vacuum concentrating, alcohol precipitating, centrifugalizing and freeze-drying to recycle the protein. The special treatment in the invention has substantially increased the free oil yield and the quality of the free oil is better than the refined oil. The water-extracting and alcohol-precipitating method to get the protein is simple and efficient; besides, the method has maintained the sunflower protein's nutritive value and functional value very well. Compared with the traditional squeezing and water-extracting technologies, the invention has many advantages such as simple producing equipments, safe operation, less energy expenditure and less pollution.

The invention discloses an isolated culture method of human amnia mesenchymal stem cells, which comprises the following steps: carrying out human placenta amnia tissue acquisition, amnia tissue disinfection, washing, shredding and mixed collagenase digestion to obtain mesenchymal stem cells, and carrying out mesenchymal stem cell in-vitro culture. The method can obtain abundant mesenchymal stem cells, which have the advantages of uniform and stable phenotype and high multiplication capacity and have the multidirectional differentiation potential, from the human amnia tissues; and the culture medium in-vitro amplification is performed to purify the cells, thereby satisfying the quantity for cryopreservation or clinical use. The method has the advantages of short cell culture period and high repetitiveness. The stem cells obtained by the method have the phenotypes of CD105<+>, CD90<+> and CD73<+>.

The invention discloses an immunosuppressor which containing a compound shown in the following general formula I and/or the general formula II, or an optical isomer thereof; wherein, R1, R2, R3, R4, R5, R6 and R7 are selected from H, OH, O-acyl, OMe, OEt, O<n>Pr, OPr, F, Cl, Br, NH2, NO2 or CN; wherein, site 5 and site 6 are an epoxy bond or a double bond; in the general formula I, the ether linkages of site 14 and site 27 are connected or disconnected; in the general formula II, the sites 14 and 15 and the sites 16 and 17 are the epoxy bond or the double bond, or a plant extract containing the compound. The immunosuppressor has stronger immuno-suppression effect, generates repression function to T and B lymphocyte periods which are excessively proliferated, and can be used for curing the self immunological diseases and relevant diseases.

The invention relates to a voice converting system and method using blind voice separation. The voice converting method comprises the steps of: applying a blind voice separation method which combines sub-band decomposition with ICA (Independent Component Analysis) in a voice input module at the front end of the voice converting system to separate a mixed signal including background noise and expected voice, and converting the expected voice obtained through separation and subjected to an unequal-length framing process to realize conversion of specific voice of people in a noise environment.

This invention is attributed to the field of organic chemistry and specifically relates to the method to synthesize drug intermediates (R)-2-amino-1-((R)-6-fluoro-3, 4-dihydrochromeno) ethanol and (R)-2-amino-1-((S)-6-fluoro-3, 4-dihydrochromeno) ethanol. The method is that, drug intermediates (2R)-2-[(1R)-4, 4-dimethyl-3, 5-dioxocyclopentyl]-6-fluoro-4-chromanone and (2S)-2-[(1R)-4, 4-dimethyl-3, 5-dioxocyclopentyl]-6-fluoro-4-chromanone are adopted as raw materials and reduced by Clemmensens method with the products reacting with p-toluenesulfonyl chloride in pyridine. The consequent products are dissolved in solvent and dry ammonia is introduced for heating reflux. The total yield can be as much as 32%.

The invention discloses a method to separate and enrich a metallic carbon tube with special electrical performance from single-walled carbon nanotubes. The method of the invention is that: a right amount of to be separated carbon nano tubes and separating promoter are added into an organic solvent, and the system is ultrasonically mixed, so that the separating promoter can fully absorb specific carbon nano tubes, then precipitation in the solution is removed and the liquid is remained; and then the carbon tubes suspending in the liquid are separated, the organic solvent is used for cleaning the carbon tubes to remove the organic that is not absorbed by the carbon tubes, thus, the product enriched with metallic carbon tubes is prepared. The separating promoter adopted by the invention is a compound with a linear condensed nucleus aromatic hydrocarbon structure, the condensed nucleus part of the compound comprises at least three aromatic rings.

The invention relates to application of a linker of polyethylene glycol and fat-soluble compounds in biological catalysis. In the invention, mPEG (methoxy polyethylene glycol) is linked with various fat-soluble compounds such as sterides, triterpene and the like by linking bridges to synthesize mPEG-modified water-soluble derivatives: OS-Z-mPEG. Via the derivatives, the original fat-soluble organic compounds can be completely dissolved in water without adding any cosolvents to perform homogeneous biological conversion or catalysis; and after reaction, by the solvent precipitation characteristic of the mPEG polymer, the converted products are selectively precipitated, filtered and collected from the fermentation liquid, thereby achieving the aims of dissolving the fat-soluble compounds and simplifying separation of the converted products.

The present invention is the preparation process of 2,3,3',4'-diphenyl tetraformic dianhydride and its derivative. Through the coupling reaction in non-proton polar solvent with 3- and 4- chloro o-dippthalate or 3- and 4-chlorophthalimide as basic material, nickel halide as catalyst, trialkylphosphine as ligand and zinc powder as reductant, diphenyl tetraformate or diphenyl tetraformyl diimide is prepared. The produced diphenyl tetraformate or diphenyl tetraformyl diimide is then hydrolyzed in NaOH solution and concentrated HCl acidified to obtain 2,3,3',4'-diphenyl tetraformic acid, which is further reflux treated in toluene or xylene or vacuum high-temperature sublimed to obtain 2,3,3',4'-diphenyl dianhydride.

The invention discloses a method for separating enriching metallic and semiconductor carbon nano-tubes from single-walled carbon nano-tubes. The method of the invention is that: first, purified carbon nano-tubes of 1 weight account that waits for separation and 0.05 to 2 weight accounts of separating accelerator are taken to be added into organic solvent for ultrasonic mixing and lead the separating accelerator to sufficiently adsorb the specific carbon nano-tube, then liquid and sediments are separated, carbon tubes that are suspended in the liquid are separated, and products of enriching metallic carbon-tubes are acquired; next, 1 weight account of sediment and 0.05 to 2 weight accounts of the separating accelerator are taken to be added into the organic solvent for ultrasonic mixing processing, then sediments and liquid are separated, carbon tubes that are suspended in the liquid are separated, and products of enriching semiconductor carbon tubes are acquired.

The invention provides a detection method for galacto-oligosaccharide based on biological enzyme technology, which belongs to the technical field of food engineering. According to the invention, at first, the method of removing proteins by using trichloroacetic acid and lead acetate is employed for pretreatment of an object to be detected, and membrane filtration is utilized to remove impurities so as to obtain a sample to be detected; then, sodium hydroxide and sodium acetate with different concentration are utilized for ion exchange and gradient elution; finally, a four-potential pulsed amperometric detector is used for detection. The invention is applicable to separation and detection of cow's milk containing galacto-oligosaccharide and a variety of galacto-oligosaccharide components in a galacto-oligosaccharide solution system; employment of ion chromatography, utilization of the methods of ion exchange and gradient elution for separation of galacto-oligosaccharide and cooperativedetection with the four-potential pulsed amperometric detector enable contamination and interference to an analytical column, the detector and the like caused by macro-molecular substances such as proteins, fat and emulsifiers which might exist in the sample to be eliminated, thereby allowing accurate and rapid detection to be achieved.

The invention belongs to the field of resource utilization of biomass waste, and particularly relates to a separation method for solid-phase biomass hydrothermal liquefaction products. The solid-phase biomass hydrothermal liquefaction products refer to solid-phase products (comprising oil-phase products and solid residues) obtained by the hydrothermal liquefaction reaction of biomasses, wherein the biomasses comprise enteromorpha, microalgae, rice straws, salix psammophila, tobacco stems, corncobs, sawdust, water hyacinths and the like. According to the method, the solid-phase biomass hydrothermal liquefaction products are mainly used as raw materials, a separation scheme of performing alkali washing and acidification and sequentially and stepwise extracting products by using organic solvents with different polarities is adopted, and the obtained products are analyzed, so that phenol, fatty acid, furan and cyclopentenone compounds can be separated in a way of combining multi-stage solution pH regulation and multi-stage stepwise extraction with the organic solvents with different polarities; the separation method is simple and convenient, environment-friendly, less in pollution, low in cost and favorable for industrial production, and the solvents can be recycled.

The invention discloses a separating system and a separating method for residual monometers in a polymer viscous liquid. The separating system comprises a gas supply device, a gas heating device, a separating device and a vacuumizing device, wherein the separating device comprises a reactor/a material filling tank, a condenser and a storage tank, wherein the reactor/the material filling tank is provided with a stirrer, and a gas dispersing pipe is mounted at the bottom of the reactor/the material filling tank. Gas provided by the gas supply device enters the gas dispersing pipe which is mounted at the bottom of the reactor/the material filling tank after passing through a gas heating device; the gas is blown to the polymer viscous liquid through the gas dispersing pipe, so that the viscous liquid is bubbled and the residual monomers are evaporated; evaporated residual monomers and the gas enter the condenser together under the action of vacuum, the residual monomers and the gas are separated by condensing; the residual monomers enter the storage tank and are stored in the storage tank. According to the separating system and the separating method, equipment is simple; process parameters are easy to control; the energy consumption is low; the processing amount is large. The separating system and the separating method can be used for separating the residual monomers from the polymer viscous liquid.

The invention provides a C-SiC micro powder separating method which is simple and environmentally friendly. According to the method, liquid the density of which is between that of C and that of SiC serves as a dispersion medium, a C-SiC micro powder mixture is added to the dispersion medium, standing and layering are conducted after full and even dispersion, C floating on the upper layer of the liquid is removed, and then the liquid dispersion medium is filtered and recovered to obtain separated SiC micro powder. Due to the fact that no chemically active substance needing to be removed is added to the separating system, no chemical reaction occurs, and pollutant emission is avoided completely. The separating method has the advantages that technology and equipment are simple, materials are cheap and easy to obtain, cost is low, yield is high and the like and is suitable for large-scale industrial production. The separated SiC micro powder is uniform in grain size and high in purity and can be widely applied to the fields such as dissociated abrasive cutting fluids and SiC abrasive wheels.