5results about How to "The separation method is simple" patented technology

This invention discloses a method and its application for isolating mitochondria from mesenchymal stem cells (MSCs) to improve their yield, purity, and activity. Compared to existing methods for isolating MSCs, the method of this invention effectively improves the yield and purity of MSCs, increasing the number of mitochondria by 2.96 times. The method also better maintains the activity of MSC mitochondria, significantly increasing cytochrome C content and exhibiting superior activity in treating autoimmune hepatitis. Furthermore, the method is simple, requires minimal reagents, and has broad application prospects in the field of mitochondrial transplantation therapy.

The application relates to the technical field of chemical synthesis, in particular to a heterogeneous catalyst which can be used for synthesizing benzyl phenylpropynoate compounds. x Ni y M z , M is at least one of Pt, Pd, Rh, Ru and Ir, x, y and z represent molar numbers, x+y+z=10, z is 1-2, and x:y is 1-8:1; and the carrier is a metal oxide or a molecular sieve. The heterogeneous catalyst can be used for synthesizing benzyl phenylpropynoate compounds by adopting a one-pot method, has high catalyst activity, good substrate universality, good stability, can be repeatedly used for more than 5 times, and can be separated by adopting a filtering mode, so that the separation method is simple. Moreover, a product post-treatment method is simple, and the target product has high yield.

The invention discloses a near-infrared fluorescent probe with an aggregation-induced emission enhancement effect. The near-infrared fluorescent probe is used for dual detection of hydrogen sulfide and hydrazine. The fluorescent probe is synthesized by carrying out Knoevenagel condensation reaction on 4-hydroxy-3-(1, 4, 5-triphenyl-1H-imidazolyl) benzaldehyde (I) and 2-(2-methyl-4H-chromene-4-subunit) malononitrile (II) under the catalytic action of piperidine, and the chemical structural formula of the fluorescent probe is as shown in the formula (III). The fluorescent probe shows high selectivity and high sensitivity to both hydrogen sulfide and hydrazine, the detection limits are as low as 50.1 nM and 89.2 nM respectively, and the fluorescent probe can be used for various scenes such as food spoilage monitoring and environmental water sample detection, can be used for biological imaging, and shows important application potential in the aspect of detection of hydrazine and hydrogen sulfide in organisms.

The invention discloses a heterogeneous catalyst, a preparation method and application of the heterogeneous catalyst in synthesis of carbonic ester compounds. The heterogeneous catalyst provided by the invention is MO-Cu-CMNDI, MO represents one of metal oxides such as MgO, Bi2O3, ZnO, CuO, Fe2O3, ZrO2, Ga2O3, CeO2, SiO2, Al2O3 and TiO2, preferably CeO2, and Cu-CMNDI represents a metal coordination network material [Cu (CMNDI) (H2O) 4] synthesized by CuSO4 and N, N '-bis (carboxyl methyl)-1, 4, 5, 8-naphthalimide (CMNDI). When the MO-Cu-CMNDI catalyst is used for the synthetic reaction of the carbonic ester compound, the catalytic activity is high, the reusability is good, and the yield of the reaction is improved.

This invention provides a method for preparing 2,5-furandicarboxylic acid in an acidic system, belonging to the field of bio-based chemical preparation. It is a method for synthesizing 2,5-furandicarboxylic acid by catalytic oxidation of 2,5-diformylfuran in an acidic environment. The method uses 2,5-diformylfuran as a raw material and employs an aqueous / organic two-phase catalytic system, using hydrogen peroxide to oxidize 2,5-diformylfuran to prepare 2,5-furandicarboxylic acid. In this invention, the raw material 2,5-diformylfuran and the catalyst are dissolved in the organic phase, the aqueous phase is acidic, and the oxidation product 2,5-furandicarboxylic acid precipitates in the aqueous phase. High-purity 2,5-furandicarboxylic acid can be obtained by simple filtration. The catalyst in the organic phase can be recycled multiple times. This invention does not use precious metal catalysts, does not require the addition of additional inorganic bases before or after the reaction, has simple product separation, generates little waste, and has broad application prospects.