402results about How to "The separation method is simple" patented technology

The invention discloses a hermetia illucens breeding system and belongs to the technical field of green cultivation. The hermetia illucens breeding system comprises a culture pond and a ceiling arranged at the upper part of the culture pond; the culture pond is sequentially provided with a water absorption layer, a heating device and a grid layer from bottom to top; the heating device comprises a heating tape and a fixing frame for fixing the heating tape; a compost is put on the upper part of the water absorption layer; the heating device is located in the compost; the grid layer is located on a surface layer of the compost; a lifting device is arranged on the side of the culture pond; the lifting device drives the heating device to lift; and a heat preservation cover is arranged at the top of the culture pond. Hermetia illucens is bred by using the hermetia illucens breeding system, so that the survival rate of hermetia illucens larvae is high and the hermetia illucens is not affected by an external environment; furthermore, the hermetia illucens is easily separated from the compost and insect feces do not need to be cleaned, so that the cost of manpower and material resources is greatly reduced. In addition, the compost in the culture pond is digested and fermented in the hermetia illucens breeding process and can be directly returned to the field as an organic fertilizer, so that the hermetia illucens breeding system is economical and environmentally friendly.

The invention relates to a method for transforming biomass sugar source into 5-hydroxymethyl-furfural, in particular to a method for preparing the 5-hydroxymethyl-furfural. The method comprises the following steps: using ionic liquid as a solvent, hexose or hexose source biomass as raw material substrates, and 0.5 to 50 percent (relative to the mass of the biomass sugar source) of acidic ionic liquid, inorganic acid or organic acid as a catalyst, and performing the reaction of materials for 1.5 minutes to 23 hours at normal pressure and at a temperature of between 80 DEG C and 100 DEG C to efficiently generate HMF. The method has the advantages of high HMF selectivity, less acid consumption, moderate operating conditions, fast reaction, reusable ionic liquid, simple process, environment protection and the like, and opens up a new approach for preparing commodity chemicals and replacing fuels starting from renewable biological resources.

The invention discloses a system and a method for industrially separating and purifying CO2 in mixed gas by a hydrate method. The system consists of a feed gas pretreatment system, a hydrate separation tower, a micro-bubble jet-loop reaction system, a column bottom hydrate decomposition system, a CO2 product collection system, a hydrate formation and decomposition heat comprehensive utilization system and a tail gas energy recovery system. The method integrates hydrate bubbling reaction technology, spraying reaction technology and micro-bubble jet reaction technology, adopts CO2 reflux displacement fractionation technology to improve the concentration of the CO2 in a hydrate phase, has simple separation technology and device and high separation rate and efficiency, comprehensively utilizes the formation heat and decomposition heat of the hydrate during refrigeration cycle, simultaneously recovers compression work of tail gas and cold energy for feed gas pretreatment, and has low energy consumption and cost during separation. The system and the method can be applied to industrial and continuous separation and purification of the CO2 in the mixed gas such as flue gas (CO2 / N2), IGCC synthetic gas (CO2 / H2), biomass synthetic gas (CO2 / H2), natural gas (CO2 / CH4), coalbed gas and the like.

The invention relates to a separation method and a culture method for umbilical cord mesenchymal stem cells. The separation method comprises the following steps: thoroughly cleaning umbilical cord tissue of a healthy newborn by using a PBS (phosphate buffer solution) containing streptomycin and penicillin, and removing blood; shearing the umbilical cord into small sections uniform in length, and mechanically separating, bluntly stripping Wharton' s jelly, and removing umbilical arteries and umbilical veins; uniformly shearing the Wharton' s jelly; re-suspending the sheared Wharton' s jelly through an MSCs (mesenchymal stem cells) culture medium, inoculating to a culture dish with laid gelatin, and putting in a CO2 culture box for cultivation; conducting centrifugal separation to obtain tissue blocks and a cell resuspension solution. The culture method comprises the following steps: enwrapping the culture dish, discarding the gelatin, and washing with the PBS; inoculating the separated out tissue blocks and the cell resuspension into the culture dish; performing digestive subculture after cell fusion growth rate reaches 80-90%.

The invention discloses a noise separation-based power transformer noise assessment method. The steps include: 1) building a voiceprint feature database in advance according to voiceprint feature information corresponding to noise signals generated by a fan component, an iron core component and a winding component when a standard power transformer is in different running states; 2) collecting in real time sound wave signals sent when a transformer to be tested runs, so as to obtain noise signals to be assessed; 3) separating noise signals generated by the fan component, the iron core component and the winding component from the noise signals to be assessed; and 4) extracting voiceprint feature information of each noise signal, identifying the running state of the transformer to be tested, and assessing the noise level of each component according to the running state. The noise separation-based power transformer noise assessment method provided by the invention can realize assessment of the noise level of each component in the power transformer utilizing acoustic characteristics, and has the advantages of easy operation implementation and high assessment precision and efficiency.

The invention discloses a composite janus microspheres based oil-water micro-emulsion separation method. With the polymers Janus microspheres with different structures and different chemical compositions as a base material, the method comprises the following steps: adsorbing magnetic nano particles on the surfaces of the microspheres through electrostatic interaction, to obtain composite Janus microspheres with magnetic responsiveness; mixing and stirring the composite Janus microspheres and an oil-water emulsion; realizing demulsification and layering of the oil-water emulsion; after the layering, applying a magnetic field to realize efficient and quick oil-water separation. According to the method disclosed by the invention, the composite Janus microspheres serve as a particle surfactanton the oil-water interface to realize the demulsification and layering of the oil-water emulsion; by applying the magnetic field, efficient and quick separation of oil and water can be realized. Theoil-water micro-emulsion separation method disclosed by the invention has the advantages of simplicity, quick process and easiness in condition control, and is applicable to efficient separation of oil water formed by different types of large-area oil.

The method relates to the method to extract the sunflower oil and recover the protein by water-enzyme method. The producing procedure comprises the following steps: dry-pulverizing the uncoated sunflower seed, adding citrate buffer to the powder and decomposing the powder by compound cellulose; centrifugalizing to get free oil, emulsion, digest and tablet; high speed centrifugalizing to get some free oil; filtering, vacuum concentrating, alcohol precipitating, centrifugalizing and freeze-drying to recycle the protein. The special treatment in the invention has substantially increased the free oil yield and the quality of the free oil is better than the refined oil. The water-extracting and alcohol-precipitating method to get the protein is simple and efficient; besides, the method has maintained the sunflower protein's nutritive value and functional value very well. Compared with the traditional squeezing and water-extracting technologies, the invention has many advantages such as simple producing equipments, safe operation, less energy expenditure and less pollution.

The invention discloses an isolated culture method of human amnia mesenchymal stem cells, which comprises the following steps: carrying out human placenta amnia tissue acquisition, amnia tissue disinfection, washing, shredding and mixed collagenase digestion to obtain mesenchymal stem cells, and carrying out mesenchymal stem cell in-vitro culture. The method can obtain abundant mesenchymal stem cells, which have the advantages of uniform and stable phenotype and high multiplication capacity and have the multidirectional differentiation potential, from the human amnia tissues; and the culture medium in-vitro amplification is performed to purify the cells, thereby satisfying the quantity for cryopreservation or clinical use. The method has the advantages of short cell culture period and high repetitiveness. The stem cells obtained by the method have the phenotypes of CD105<+>, CD90<+> and CD73<+>.

The invention discloses an immunosuppressor which containing a compound shown in the following general formula I and / or the general formula II, or an optical isomer thereof; wherein, R1, R2, R3, R4, R5, R6 and R7 are selected from H, OH, O-acyl, OMe, OEt, O<n>Pr, OPr, F, Cl, Br, NH2, NO2 or CN; wherein, site 5 and site 6 are an epoxy bond or a double bond; in the general formula I, the ether linkages of site 14 and site 27 are connected or disconnected; in the general formula II, the sites 14 and 15 and the sites 16 and 17 are the epoxy bond or the double bond, or a plant extract containing the compound. The immunosuppressor has stronger immuno-suppression effect, generates repression function to T and B lymphocyte periods which are excessively proliferated, and can be used for curing the self immunological diseases and relevant diseases.

The invention relates to a voice converting system and method using blind voice separation. The voice converting method comprises the steps of: applying a blind voice separation method which combines sub-band decomposition with ICA (Independent Component Analysis) in a voice input module at the front end of the voice converting system to separate a mixed signal including background noise and expected voice, and converting the expected voice obtained through separation and subjected to an unequal-length framing process to realize conversion of specific voice of people in a noise environment.

This invention is attributed to the field of organic chemistry and specifically relates to the method to synthesize drug intermediates (R)-2-amino-1-((R)-6-fluoro-3, 4-dihydrochromeno) ethanol and (R)-2-amino-1-((S)-6-fluoro-3, 4-dihydrochromeno) ethanol. The method is that, drug intermediates (2R)-2-[(1R)-4, 4-dimethyl-3, 5-dioxocyclopentyl]-6-fluoro-4-chromanone and (2S)-2-[(1R)-4, 4-dimethyl-3, 5-dioxocyclopentyl]-6-fluoro-4-chromanone are adopted as raw materials and reduced by Clemmensens method with the products reacting with p-toluenesulfonyl chloride in pyridine. The consequent products are dissolved in solvent and dry ammonia is introduced for heating reflux. The total yield can be as much as 32%.

The invention relates to a C4 hydrocarbon catalysis and separation method capable of separating isobutene and butene-2, mainly solving the technical problems that in the prior art, the rate of double-bond isomerism of butane-1 is low, the purity of isobutene is low and the separation method is complicated. The method of the invention comprises the following steps: feeding C4 fraction containing butane-1, butene-2 and isobutene and hydrogen in a rectifying tower reactor containing one or two Ni-based catalyst beds, using butadiene in C4 fraction to prepare butene-2 through selective hydrogenation, or using butadiene to react with thiol compound in C4 fraction and prepare thioether with higher boiling point, performing hydroisomerization of the double bond of butane-1 to approach the equilibrium concentration of butene-2, continuously breaking the thermodynamic equilibrium between butane-1 and butene-2 through the rectification process, separating butane-1, butene-2 and sulfur-containing compound with isobutene, using isobutene as overhead product to remove and removing the residual material from the tower bottom. Therefore, the problems are better solved and the method can be used in the industrial production of using ethylene plants to crack C4 hydrocarbon or the industrial production of using refinery C4 hydrocarbon to prepare high-purity isobutylene.

The invention relates to a method for preparation of polyoxymethylene dimethyl ether from paraformaldehyde, in particular to a method for preparation of polyoxymethylene dimethyl ether in the presence of an acidic resin catalyst and separation of polyoxymethylene dimethyl ether. The method adopts methylal and paraformaldehyde as raw materials to prepare polyoxymethylene dimethyl ether under mild reaction conditions. The reaction product is first subjected to molecular sieve adsorption, then to a reaction with an alkaline substance, and further to atmospheric and vacuum distillation so as to separate low-boiling point by-products and polyoxymethylene dimethyl ether (DMM3-8) as well as higher polymerization degree polyoxymethylene dimethyl ether (DMMn, with n being larger than 8), wherein the low-boiling point part and the high-boiling point part further react to prepare polyoxymethylene dimethyl ether. The separated polyoxymethylene dimethyl ether (DMM3-8) product can be directly used as a component for co-combustion with diesel.

The invention discloses a method to separate and enrich a metallic carbon tube with special electrical performance from single-walled carbon nanotubes. The method of the invention is that: a right amount of to be separated carbon nano tubes and separating promoter are added into an organic solvent, and the system is ultrasonically mixed, so that the separating promoter can fully absorb specific carbon nano tubes, then precipitation in the solution is removed and the liquid is remained; and then the carbon tubes suspending in the liquid are separated, the organic solvent is used for cleaning the carbon tubes to remove the organic that is not absorbed by the carbon tubes, thus, the product enriched with metallic carbon tubes is prepared. The separating promoter adopted by the invention is a compound with a linear condensed nucleus aromatic hydrocarbon structure, the condensed nucleus part of the compound comprises at least three aromatic rings.

The invention relates to application of a linker of polyethylene glycol and fat-soluble compounds in biological catalysis. In the invention, mPEG (methoxy polyethylene glycol) is linked with various fat-soluble compounds such as sterides, triterpene and the like by linking bridges to synthesize mPEG-modified water-soluble derivatives: OS-Z-mPEG. Via the derivatives, the original fat-soluble organic compounds can be completely dissolved in water without adding any cosolvents to perform homogeneous biological conversion or catalysis; and after reaction, by the solvent precipitation characteristic of the mPEG polymer, the converted products are selectively precipitated, filtered and collected from the fermentation liquid, thereby achieving the aims of dissolving the fat-soluble compounds and simplifying separation of the converted products.

The present invention is the preparation process of 2,3,3',4'-diphenyl tetraformic dianhydride and its derivative. Through the coupling reaction in non-proton polar solvent with 3- and 4- chloro o-dippthalate or 3- and 4-chlorophthalimide as basic material, nickel halide as catalyst, trialkylphosphine as ligand and zinc powder as reductant, diphenyl tetraformate or diphenyl tetraformyl diimide is prepared. The produced diphenyl tetraformate or diphenyl tetraformyl diimide is then hydrolyzed in NaOH solution and concentrated HCl acidified to obtain 2,3,3',4'-diphenyl tetraformic acid, which is further reflux treated in toluene or xylene or vacuum high-temperature sublimed to obtain 2,3,3',4'-diphenyl dianhydride.

Provided herein is a novel process for the preparation of triazolo[4,5-d]pyrimidine derivatives. Provided particularly herein is a novel, commercially viable and industrially advantageous process for the preparation of highly pure ticagrelor or a pharmaceutically acceptable salt thereof. Provided further herein is a novel process for the preparation of substituted cyclopentanamine derivatives, which are useful intermediates in the preparation of triazolo[4,5-d]pyrimidine compounds. Provided particularly herein is a novel, commercially viable and industrially advantageous process for the preparation of a ticagrelor intermediate, 2-[[(3aR,4S,6R,6aS)-6-amino-2,2-dimethyltetrahydro-3aH-cyclopenta[d][1,3]-dioxol-4-yl]oxy]-1-ethanol.

The invention discloses a method for separating enriching metallic and semiconductor carbon nano-tubes from single-walled carbon nano-tubes. The method of the invention is that: first, purified carbon nano-tubes of 1 weight account that waits for separation and 0.05 to 2 weight accounts of separating accelerator are taken to be added into organic solvent for ultrasonic mixing and lead the separating accelerator to sufficiently adsorb the specific carbon nano-tube, then liquid and sediments are separated, carbon tubes that are suspended in the liquid are separated, and products of enriching metallic carbon-tubes are acquired; next, 1 weight account of sediment and 0.05 to 2 weight accounts of the separating accelerator are taken to be added into the organic solvent for ultrasonic mixing processing, then sediments and liquid are separated, carbon tubes that are suspended in the liquid are separated, and products of enriching semiconductor carbon tubes are acquired.

The invention relates to two photon polymerization initiating system, especially relates to a high- effective method for synthesizing coumarins dyestuff connected with stilbene and its application in two photon polymerization. It comprises following steps: dissolving the mediate coumarins dyestuff with A1 gene into organic solvent; adding alkaline catalyst solution into it; dropping the organic phosphonic solvent carrying A2 gene with the same molar weight to that of coumarins dyestuff into reation system, heating to solvent boiling and refluxing; cooling and separting organic phase from water phase through extraction with organic solvent; drying and evaporating organic phase, separating coarse product with column chromatography, heating with iodine with its amount the same to that of catalyst, getting coumarins dyestuff connected with stilbene, which is used as photosensitiser in photon polymerization, and its structure is as follows: the substituents of A1 and A2 are the same or different, and are chose from alkyl, alkoxy, amido, heterocycle or substituted phenyl.

The invention provides an identification and separation device for waste plastics. The device comprises a crushing plant, a friction assembly, an accelerating unit, a constant magnetic field unit, a comparing unit, and a collecting unit. A variety of standard pure plastics or waste plastics are crushed by the crushing plant and then are charged by the friction assembly, the charged plastics enter into the accelerating unit for acceleration and then enter into a constant magnetic field at a same speed with an included angle formed by the track of the plastics and the direction of the magnetic field being greater than zero, thereby forming different movement tracks, the comparing unit compares the movement tracks of waste plastic particles with the movement tracks of a variety of standard pure plastic particles so as to determine that a waste plastic and a standard pure plastic which has a same movement track as the waste plastic does are of a same kind, and the collecting unit collects waste plastic particles which have similar movement tracks in the magnetic field. The invention also provides an identification and separation method for waste plastics. According to the invention, the disadvantages that an optical method for identification of waste plastics is work-environment-demanding and expensive can be overcome, and the purposes of easy operation and low cost in identification and separation of waste plastics of automobiles are achieved.

The invention provides a detection method for galacto-oligosaccharide based on biological enzyme technology, which belongs to the technical field of food engineering. According to the invention, at first, the method of removing proteins by using trichloroacetic acid and lead acetate is employed for pretreatment of an object to be detected, and membrane filtration is utilized to remove impurities so as to obtain a sample to be detected; then, sodium hydroxide and sodium acetate with different concentration are utilized for ion exchange and gradient elution; finally, a four-potential pulsed amperometric detector is used for detection. The invention is applicable to separation and detection of cow's milk containing galacto-oligosaccharide and a variety of galacto-oligosaccharide components in a galacto-oligosaccharide solution system; employment of ion chromatography, utilization of the methods of ion exchange and gradient elution for separation of galacto-oligosaccharide and cooperativedetection with the four-potential pulsed amperometric detector enable contamination and interference to an analytical column, the detector and the like caused by macro-molecular substances such as proteins, fat and emulsifiers which might exist in the sample to be eliminated, thereby allowing accurate and rapid detection to be achieved.

The invention discloses a flow measuring device for gas-liquid two-phase fluid and a measuring method thereof. The flow measuring device mainly consists of a horizontal pipeline, a liquid collection box, a gas collection box, a gas flow meter and a liquid flow meter; when flowing through the horizontal pipeline, the gas-liquid two-phase fluid generates natural separation under the action of gravity thereof, i.e. the liquid is deposited on the bottom of the pipeline, and the gas is gathered on the upper part of the pipeline; the liquid is collected into the liquid collection box through the porous wall of the lower part of the pipeline, and then is measured by the liquid flow meter; and simultaneously, part of the gas is collected into the gas collection box through the porous wall of the upper part of the pipeline, then is measured by the gas flow meter, and then is subject to conversion based on the proportional relationship to obtain the total gas flow. By adopting the invention, the separation method is simplified, the volume of the whole measuring device is greatly reduced, the measurement instantaneity is improved, and the automaticity is improved, thus the invention is convenient to be applied to engineering.

The invention relates to the field of image segmentation, in particular to a cell separation method. The cell separation method comprises the following steps: A, pre-segmenting a cell image by an OSTU method; B, performing hole filling on the pre-segmented image by a boundary tracking method; C, performing watershed segmentation on the image subjected to hole filling; and d, combining 'over-segmentation' regions of the segmented image and re-segmenting 'incomplete segmentation' regions. Compared with the conventional segmentation algorithm synthesizing concave point analysis and boundary detection and the conventional segmentation algorithm based on mathematical morphology, the cell separation method is clear in thinking and easy to implement in codes, so that the cell separation method is easy to implement. Compared with the traditional method, the cell separation method can obviously reduce the over-segmentation conditions.

Under the condition of constant temperature the prepared solid super acidic catalyst is added into the acetone solution, under the protection of nitrogen gas the pseudo ionone can be slowly added while stirring, after the reaction is completed, the solid super acidic catalyst is fertered and separated, then the acetone can be separated out in rotary evaporator, finally, the target product beta-ionone can be separated out by utilizing reduced pressure distillation process.

The invention relates to a synthesis method for (6R, 12aR)-6-(1, 3-benzodioxane heterocyclas-5-base)-2-methyl-2, 3, 6, 7, 12, -12a-hexahydro pyrazinyl [1', 2':1, 6] pyridyl [3, 4-b] indole-1, 4-diketone in the technical field of the pharmaceutical engineering. With the D-methyl tryptophan and piperonal as raw materials, the cis-1, 3-disubstituted carboline intermediates are prepared and generated through the Pictet-Spengler cyclization and then the products called tadalafil are obtained through the acylation ammoniation of the 3-secondary amino group and the final MTX substitution and condensation close-loop. By adopting the acidic condition protic solvents in the invention, the trans-1, 3-disubstituted carboline intermediates generated through the Pictet-Spengler cyclization, which is a key step in the existing process, are converted into those with a cis-configuration, thus greatly enhancing the stereospecific configuration yield of the carboline intermediates so as to enhace the overal yield of the tadalafil with reaching the goal of optimizing and improving the synthesis process.

The invention belongs to the field of resource utilization of biomass waste, and particularly relates to a separation method for solid-phase biomass hydrothermal liquefaction products. The solid-phase biomass hydrothermal liquefaction products refer to solid-phase products (comprising oil-phase products and solid residues) obtained by the hydrothermal liquefaction reaction of biomasses, wherein the biomasses comprise enteromorpha, microalgae, rice straws, salix psammophila, tobacco stems, corncobs, sawdust, water hyacinths and the like. According to the method, the solid-phase biomass hydrothermal liquefaction products are mainly used as raw materials, a separation scheme of performing alkali washing and acidification and sequentially and stepwise extracting products by using organic solvents with different polarities is adopted, and the obtained products are analyzed, so that phenol, fatty acid, furan and cyclopentenone compounds can be separated in a way of combining multi-stage solution pH regulation and multi-stage stepwise extraction with the organic solvents with different polarities; the separation method is simple and convenient, environment-friendly, less in pollution, low in cost and favorable for industrial production, and the solvents can be recycled.

The invention discloses a method for separating long terminal repeats of retrotransposons. Genome DNA is taken as a template, an RNaseH1 primer is respectively combined with different annealing control primers for first polymerase chain reaction (PCR) reaction, the first PCR product is diluted to serve as a template, an RNaseH2 primer and a universal primer UP are subjected to secondary PCR reaction amplification, conversion and extraction of plasma DNA are performed, and a sequence is subjected to sequencing to obtain an amino acid sequence of RNaseH enzyme, and proteins are encoded. Compared with the common PCR technology, the method has the advantages that: the operation is simple, long terminal repeat regions of the retrotransposons are easily aligned, and the pertinence is high; compared with a flow type magnetic microbead inverse hybrid method, the method has the advantages of saving hybrid, eluting, screening and other processes, simplifying the flow and having high efficiency;and compared with other separation methods, the method has the advantages of reducing time consumption, quickly obtaining target segments, cloning the long terminal repeats in short time, and reducing false positive rate.

The invention discloses a separating system and a separating method for residual monometers in a polymer viscous liquid. The separating system comprises a gas supply device, a gas heating device, a separating device and a vacuumizing device, wherein the separating device comprises a reactor / a material filling tank, a condenser and a storage tank, wherein the reactor / the material filling tank is provided with a stirrer, and a gas dispersing pipe is mounted at the bottom of the reactor / the material filling tank. Gas provided by the gas supply device enters the gas dispersing pipe which is mounted at the bottom of the reactor / the material filling tank after passing through a gas heating device; the gas is blown to the polymer viscous liquid through the gas dispersing pipe, so that the viscous liquid is bubbled and the residual monomers are evaporated; evaporated residual monomers and the gas enter the condenser together under the action of vacuum, the residual monomers and the gas are separated by condensing; the residual monomers enter the storage tank and are stored in the storage tank. According to the separating system and the separating method, equipment is simple; process parameters are easy to control; the energy consumption is low; the processing amount is large. The separating system and the separating method can be used for separating the residual monomers from the polymer viscous liquid.

The invention provides a C-SiC micro powder separating method which is simple and environmentally friendly. According to the method, liquid the density of which is between that of C and that of SiC serves as a dispersion medium, a C-SiC micro powder mixture is added to the dispersion medium, standing and layering are conducted after full and even dispersion, C floating on the upper layer of the liquid is removed, and then the liquid dispersion medium is filtered and recovered to obtain separated SiC micro powder. Due to the fact that no chemically active substance needing to be removed is added to the separating system, no chemical reaction occurs, and pollutant emission is avoided completely. The separating method has the advantages that technology and equipment are simple, materials are cheap and easy to obtain, cost is low, yield is high and the like and is suitable for large-scale industrial production. The separated SiC micro powder is uniform in grain size and high in purity and can be widely applied to the fields such as dissociated abrasive cutting fluids and SiC abrasive wheels.

The invention provides a mass isolation method of a natural sweetener Trilobatin. The method comprises the following steps: adding a crushed Trilobatin raw material into 30-90% (by volume) of ethanol according to the solid-to-liquid mass ratio of 1:10-50, carrying out thermal reflux for separation, filtering a separating medium to obtain filter residue and a filtrate, and carrying out vacuum concentration on the filtrate until there is no alcohol taste so as to obtain an isolate; dissolving the isolate by adding water, extracting with ethyl acetate, and carrying out vacuum concentration on an extract liquor to obtain an ethyl acetate extract; and separating the ethyl acetate extract by high-speed countercurrent chromatography so as to obtain the natural sweetener. The isolation method is simple and fast and has short isolation cycle. Monomers preparation amount is large, and monomers have multiple pharmacological activities.