609results about How to "Easy to purify" patented technology

The invention discloses a recombinant XII type collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant XII type collagen is any one of (A1)-(A3); (A1) is an amino acid sequence as shown in SEQ ID NO: 1; (A2) is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1 and has the same functional protein; and (A3) is an amino acid sequence of a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the sequence (A1) or (A2). The recombinant XII type collagen provided by the invention has good antioxidant activity, and has a repairing effect on the problems of skin laxity, wrinkle increase and the like caused by degradation of type I collagen fibers of aged skin.

The invention relates to the technical field of bioengineering, and discloses a mutant of Burkholderia lipase as well as a recombinant expression vector and application of the mutant, the mutant comprises P131S, T132A, T251A, T251S, P131A, P131Q, T132G, Q88S and P113A single processes; and a Q88S double process and a P113A double process; and a multi-site mutation combination: a combination I: Q88S, P113A, T132G and L287S, and a combination II: Q88S, P113A, T132G and L287S; a combination II: Q88S, P113A, P131S and L287S; and a combination III: Q88S, P113A, P131S and T251A. Based on experiments, the invention finds that each mutant can not only improve the protein yield, but also improve the enzyme activity by 57% at most; ePA enrichment experiments find that the EPA content of the mutant can be increased by 33% at most, and the mutant has excellent EPA purification and enrichment effects.

The invention relates to a synthesis method of 1, 2-bis (4-vinyl phenyl) ethane, which comprises the following steps: 1) under the action of magnesium or zinc, carrying out self-coupling reaction on benzyl halide at a benzyl position to obtain 1, 2-bis (4-halogen phenyl) ethane, and 2) preparing the 1, 2-bis (4-vinyl phenyl) ethane from the 1, 2-bis (4-halogen phenyl) ethane, which is selected from any one of the following schemes: 2i: carrying out self-coupling reaction on the 1, 2-bis (4-vinyl phenyl) ethane obtained in the step 1) to obtain the 1, 2-bis (4-vinyl phenyl) ethane. The preparation method comprises the following steps: preparing a Grignard reagent from 1, 2-bis (4-halogen phenyl) ethane, and coupling the Grignard reagent with vinyl chloride to prepare 1, 2-bis (4-vinyl phenyl) ethane; and (2iii) coupling the 1, 2-bis (4-halogen phenyl) ethane obtained in the step (1) with vinyl magnesium chloride to prepare the 1, 2-bis (4-vinyl phenyl) ethane. According to the invention, the 1, 2-bis (4-vinyl phenyl) ethane is prepared from p-benzyl halide through two-step reaction at a relatively high yield, and the yield of the two-step reaction can reach 86.9%.

The invention provides a process for preparing various nutritional ingredients from oats, and relates to the technical field of deep processing of oats. The process comprises the following steps: crushing and sieving oats to obtain oat bran; the method comprises the following steps: extracting oat bran through a supercritical extraction technology to obtain pretreated oat bran and an extract, and carrying out gradient membrane treatment and membrane ultrafiltration on the extract to obtain oat oil; extracting the pretreated oat bran through continuous sectional extraction equipment; after first-stage protein extraction, second-stage dextran extraction, third-stage immobilized enzymolysis and fourth-stage filler decoloration, the materials enter a system balance tank, and membrane separation and concentration are performed to obtain the oat beta-dextran. According to the invention, various nutrient substances such as oat beta-glucan, oat protein, oat oil and the like can be extracted at the same time, the defects in the prior art are overcome, the automation degree is high, and energy conservation and emission reduction are realized.

The invention relates to the technical field of recombinant protein expression, in particular to recombinant human-derived III-type collagen as well as a preparation method and application thereof. The invention provides a recombinant human source III type collagen. The amino acid sequence of the recombinant human source III type collagen is shown as SEQ ID NO. 1. The recombinant human-derived III-type collagen prepared by the invention can be better applied to a human body, better exerts biological functions of the collagen, avoids repulsive interaction, is high in safety, can effectively promote cell proliferation and oxidation resistance, and is high in expression yield, stable in batches and good in quality; the recombinant human III type collagen prepared by the invention has the potential of being applied to preparation and production of biological medicines and cosmetics.

The invention relates to the technical field of medicine preparation, and discloses a preparation method of tirpotide, which comprises the following steps: S1, taking 10mmol of RinkAmide-AmResin, adding into a reaction column, and swelling for 30 minutes by using DMF (Dimethyl Formamide); s2, a deprotection mode is as follows: DBLK deprotection is performed twice for 5 min and 15 min respectively, and the resin is washed once with DMF in the middle; and S3, after deprotection, washing the resin with DMF for 5 times, taking a small amount of resin, and determining the completion of Fmoc deprotection through a Kaiser color test. The method effectively solves the problems that the preparation process is more, the process is tedious and the cost is high when a fragment method is adopted for synthesis, and the purification effect is influenced by most impurities difficult to remove; the purification process is simple, and a sample with the purity larger than 99.0% and the single impurity smaller than 0.2% can be obtained through two-step HPLC purification; a novel salt exchange method is provided, after a purified qualified sample solution is subjected to sedimentation centrifugation and washing centrifugation desalination, various salts can be added into the product, and then the product in other salt forms of the tilpotide is obtained through freeze-drying.

The invention discloses a rare-earth metal arylamine group compound as well as preparation method and application thereof. The invention discloses the rare-earth metal arylamine group compound which is characterized in that a general formula of the rare-earth metal arylamine group compound is (2,6-R12PhNH)5LnLi2(THF)2, wherein Ln is a rear-earth metal selected from one of neodymium, samarium, ytterbium or yttrium; R1 is selected from one of hydrogen, methyl or isopropyl. The rare-earth metal arylamine group compound has the advantages that the rare-earth metal arylamine group compound has a definite structure; raw materials used in the preparation method are simple, easily obtained and cheap; the reaction process is simple and easily operated; the rare-earth metal arylamine group compound product is conveniently purified and the yield of the product is high; the rare-earth metal arylamine group compound has a high activity when acting as a single component catalyst to catalyze a hydrogen phosphine reaction of aldehyde or ketone and phosphite ester; the use amount of the catalyst is less; the yield is high; a substrate has wide universality; a new and simple method is provided for synthesis of a-hydroxyphosphonate.

The invention relates to the technical field of chemical synthesis, and particularly provides a preparation method of a semaglutide side chain, which comprises the following steps: step S1, carrying out reaction on octadecanedioic acid mono-tert-butyl ester and trifluoroacetic acid pentafluorophenyl ester to generate a first intermediate; s2, enabling the first intermediate to react with 1-tert-butyl L-glutamic acid to generate a second intermediate; s3, enabling the second intermediate to react with pentafluorophenyl trifluoroacetate to generate a third intermediate; and S4, carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid, so as to generate a semaglutide side chain, and carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid. According to the preparation method disclosed by the embodiment of the invention, the pentafluorophenyl trifluoroacetate is used as the condensation reagent for two times to activate the carboxylic acid, and the amidation reaction is carried out for two times to construct the amido bond after each activation, so that compared with the traditional carbodiimide and urea positive ion type condensation reagent, the chiral racemization in the condensation process can be effectively avoided, and the yield of the product is improved. The condensation chiral racemization impurity can be lower than 0.3%.

The invention provides a synthesis method of 6-(t-butyloxycarboryl)-3, 6-diazabicyclo [3.1. 1] heptane, which comprises the following steps: starting from N-benzyl-2, 6-piperidinedione, firstly forming a dibromide 1, then forming a bridge ring intermediate 2 under alkaline conditions, then removing benzyl to obtain an intermediate 3, then reducing dicarbonyl to form free amine 4, and finally synthesizing the 6-(t-butyloxycarboryl)-3, 6-diazabicyclo [3.1. 1] heptane. Then performing Boc protection on amino to obtain an intermediate 5, and performing debenzylation to form a target product. According to the synthesis method, few impurities are generated, and the product is easy to purify and high in purity, so that the total yield is increased, the unit cost is effectively reduced, and large-scale industrial production is facilitated.

The invention provides a protein for synthesizing an octadecene compound and a method for synthesizing the octadecene compound. Relates to the field of enzymology. The amino acid sequence of the protein has more than 95% of consistency with the sequence as shown in SEQ ID No.3. The protein, hexadecanoic acid specific thioesterase, Z11 desaturase, gene silencing suppressor and vegetable fat synthesis related transcription factors are jointly expressed in plants and can be used for synthesizing (Z)-13-octadecenoic acid.

The invention belongs to the field of biological medicine, and relates to an anti-SEZ6 nano antibody or antigen binding fragment and application. The anti-SEZ6 nano antibody or the antigen binding fragment comprises three complementary determining regions, namely, CDR1, CDR2 and CDR3; the sequences of all the regions are respectively shown as SEQ ID NO: 1 to SEQ ID NO: 18. Compared with a conventional antibody, the anti-SEZ6 nano antibody is efficient in expression, simple in purification, small in molecular weight, high in affinity, relatively good in specificity, high in solubility and strong in tolerance, and can be widely applied to the fields of scientific research, diagnosis and treatment.

The invention relates to the technical field of organic photoelectric materials, in particular to an acceptor material containing a cyclohexyl side chain, the structural formula of the acceptor material containing the cyclohexyl side chain is one of Y-C1 or Y-C2, and the acceptor material containing the cyclohexyl side chain is # imgabs0. On the basis of a traditional A-D-A structure with a Y series analogue as a core, a large-volume steric hindrance side chain is introduced, and a large-volume cyclohexyl group is introduced into the side chain, so that the acceptor material containing the cyclohexyl side chain is obtained. The organic small molecule acceptor material has the advantages that the steric effect is increased, the intramolecular and intermolecular interaction is adjusted, the polarizability of molecules is enhanced, the intermolecular electron orbital overlapping is promoted, the delocalization of charge transfer pathways is promoted, the energy loss is further reduced, and the efficiency is improved; the material has excellent charge transfer characteristic and good intermolecular accumulation effect, is beneficial to the improvement of charge mobility, and has better photovoltaic performance in the test of a ternary organic solar cell.

An in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply relates to the field of solid waste resource utilization and renewable energy technology. The purpose of the present invention is to solve the problems of low hydrogen solubility and insufficient carbon dioxide bioconversion in the biogas purification process, as well as the inhibitory effect of excessive hydrogen dosage on biogas production by anaerobic digestion of sludge. The present invention utilizes the anaerobic digestion process of sludge in an anaerobic environment to produce biogas, and adds hydrogen to the process by direct hydrogen input or zero-valent iron corrosion, so that hydrogen penetrates into the liquid phase. The hydrogenotrophic methanogens in the system use hydrogen as an electron donor and carbon dioxide in the biogas produced by anaerobic digestion of sludge as an electron acceptor to convert hydrogen and carbon dioxide into methane to achieve in-situ biogas purification. The present invention can obtain an in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply.

The invention provides a synthesis method of tilpotide. The synthesis method comprises the following steps: carrying out a reaction on Fmoc-Linker-OH, HBTU and a swelling AM Resin to prepare Fmoc-Linker-AM Resin, and carrying out a reaction on the Fmoc-Linker-OH and the HBTU and the swelling AM Resin to prepare the tilpotide. Adding Fmoc-Ser (tBu)-OH and HOBT into a reaction system of the resin and the PIP / DMF, and completely reacting; sequentially completing coupling from the C end to the N end to obtain full-protection peptide resin; adding a cracking reagent into the full-protection peptide resin, reacting, collecting filtrate, cooling, precipitating, and drying to obtain crude peptide; the problem that in the prior art, a linker on MBHA Resin is broken to generate new impurities is solved. The defect of using heavy metal palladium or genotoxic substance hydrazine hydrate is avoided; the problem that [-(Pro-Pro)] deletion impurities are generated by step-by-step coupling of C-terminal Pro36, Pro37 and Pro38 is solved; finished products are saved, and industrial production is facilitated.

The utility model relates to the technical field of isotope separation, in particular to a system for enriching and purifying helium-3 in liquid helium. The system comprises a refrigeration unit and an enrichment unit, the refrigeration unit is of a nested structure and sequentially comprises an external Dewar (1), a cold shield (2) and an internal Dewar (3) from outside to inside; the enrichment unit comprises a heater (4) and a shell (5); the shell (5) comprises a linear section and an arc-shaped section which are connected from top to bottom; and the heater (4) is arranged in the cavity of the shell (5) and corresponds to the linear section. The system for enriching helium-3 from liquid helium below the helium-4 superfluid transformation temperature is designed by enriching firstly and then rectifying based on the superfluid characteristic of helium-4 and applying the thermomechanical effect of superfluid helium, it is predicted that the helium-3 concentration is increased from 10 <-7 > magnitude order to 10 <-3 > magnitude order, and subsequent helium-3 purification is facilitated.

The invention discloses a mycoplasma hyopneumoniae multi-antigen fusion protein and a vaccine composition and application thereof, and belongs to the technical field of genetic engineering and vaccines. The fusion protein comprises an escherichia coli heat-labile enterotoxin LTB, a mycoplasma hyopneumoniae antigen protein P97R1, a mycoplasma hyopneumoniae lipoprotein 418 and a mycoplasma hyopneumoniae surface antigen protein P46 from the N terminal to the C terminal in the sequence. The vaccine composition comprises the fusion protein and an adjuvant acceptable in veterinary medicine. A vaccine prepared from the fusion protein has the advantages of higher pertinence and immunogenicity, the cell and humoral immune response level is remarkably improved, and meanwhile, the fusion protein is easy to purify and prepare and suitable for large-scale industrial production.

The invention relates to an ardisia crenata carbon glycosyl transferase AcCGT64 gene and application of the ardisia crenata carbon glycosyl transferase AcCGT64 gene in preparation of bergenin, and belongs to the technical field of biology. The nucleotide sequence of the ardisia crenata carbon glycosyl transferase AcCGT64 gene is as shown in SEQ ID NO. 1, and the total length of the sequence is 1359 bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 451 amino acid residues are encoded. The ardisia crenata carbon glycosyl transferase AcCGT64 gene disclosed by the invention can be used as a biosynthesis regulation gene of bergenin, is applied to preparation of bergenin, and is remarkable in application prospect and easy to popularize and apply.

The invention relates to a giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene and application thereof in preparation of bergenin, and belongs to the technical field of biology. The nucleotide sequence of the giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene is as shown in SEQ ID NO.1, and the total length of the sequence is 1095bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 364 amino acid residues are encoded. The giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene disclosed by the invention can be used as a biosynthesis regulation gene of bergenin, is applied to preparation of bergenin, and is remarkable in application prospect and easy to popularize and apply.

The invention relates to the technical field of nucleic acid extraction and purification, in particular to a DNA and RNA co-extraction reaction reagent and an extraction method, the reaction reagent comprises a nucleic acid protective agent RP, a sample lysis binding solution LB, a protein washing solution WB1, a nucleic acid rinsing solution WB2 and a nucleic acid eluent EB; the nucleic acid protective agent RP comprises DTT, mercaptoethanol, TCEP and sodium hydroxide; the sample cracking binding solution LB comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, SDS, potassium chloride, sodium chloride, bromhexine, N-acetylcysteine, ambroxol hydrochloride, isopropanol and absolute ethyl alcohol; the protein washing liquid WB1 comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, sodium chloride, isopropanol and absolute ethyl alcohol; the nucleic acid rinsing liquid WB2 is prepared from Tris-HCl, sodium chloride, isopropanol and absolute ethyl alcohol; and the nucleic acid eluent EB is nuclease-free water. The reaction reagent can be used for rapidly and sufficiently enriching and purifying DNA and RNA of a sample at the same time.

The invention provides a preparation method of cresol trozole trisiloxane, and belongs to the technical field of sun-screening agent preparation. The preparation method of cresol trozole trisiloxane comprises the following steps: carrying out Claisen rearrangement reaction on a concentrated solution of a compound as shown in a formula II to obtain a compound as shown in a formula III; and reacting the compound shown in the formula III with heptamethyltrisiloxane to obtain a compound shown in a formula IV. The concentrated solution of the compound shown in the formula II is subjected to the Claisen rearrangement reaction under the solvent-free condition to prepare the compound shown in the formula III, the reaction time for preparing the compound shown in the formula III is shortened, the reaction efficiency for preparing the compound shown in the formula III is improved, the yield for preparing the compound shown in the formula III is increased, the reaction time for preparing cresol trozole trisiloxane is shortened, and the yield of cresol trozole trisiloxane is increased. The reaction efficiency of cresol trozole trisiloxane preparation is improved, and the yield of cresol trozole trisiloxane preparation is improved.

The invention provides a preparation method of high-purity molybdenum trioxide, which belongs to the technical field of molybdenum trioxide production and comprises the following steps: S1, crushing, grinding and roasting molybdenum ore sand to obtain crude molybdenum oxide, adding the crude molybdenum oxide into a sodium hydroxide solution, heating, stirring and filtering to obtain a crude sodium molybdate solution; s2, adjusting the pH value of the crude sodium molybdate solution to 5.5-7.0, introducing the crude sodium molybdate solution into an exchange column of macroporous cation exchange resin, then adjusting the pH value of the solution to 7.0-8.5, and introducing the crude sodium molybdate solution into an exchange column filled with anion exchange resin to obtain a high-purity sodium molybdate solution; s3, excessive ammonia water is added into the high-purity sodium molybdate solution, ammonium molybdate is separated out, washing and drying are conducted, and high-purity ammonium molybdate is obtained; and S4, the high-purity ammonium molybdate is put into a rotary calcining furnace, rare earth oxide is sprayed on the inner wall of a furnace tube, and the high-purity molybdenum trioxide is prepared by adopting a calcining mode of firstly heating, then cooling and then heating. According to the invention, the purpose of producing high-purity molybdenum trioxide is realized.

The invention relates to the field of separation and purification, and discloses a norbornene separation and purification method and system. The method provided by the invention is suitable for separation and purification of the norbornene synthesis liquid obtained by a solvent method, high-purity norbornene can be obtained in two steps, and meanwhile, a solvent which can be reused in a norbornene synthesis process can be directly obtained. According to the method, high-purity norbornene suitable for COC synthesis can be obtained at a high yield, and the method has the advantages of being short in technological process, high in resource utilization rate, reliable in process, easy to manage, easy to industrially popularize and the like.

The invention relates to a preparation method of lithium fluoroborate containing unsaturated bonds, and belongs to the technical field of lithium ion battery additives. The preparation method comprises the following steps: carrying out reaction on organic acid lithium containing unsaturated bonds and boron trifluoride gas in a solvent at a low temperature of-10 to 0 DEG C, and carrying out solid-liquid separation to obtain a lithium fluoroborate crude product containing unsaturated bonds; the solvent is a poor solvent of lithium fluoroborate containing unsaturated bonds. According to the method, boron trifluoride gas is used as a fluorine reagent, so that introduction of water is avoided; the method has the advantages of simple operation, high product quality, high yield, low cost, full utilization of raw materials, reduction of the generation of three wastes, and suitableness for large-scale production. The yield of the obtained product can reach 96% or above, and the purity can reach 99% or above.

The invention discloses a porcine delta-coronavirus double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) detection kit based on an S protein monoclonal antibody as well as a use method and application of the porcine delta-coronavirus double-antibody sandwich ELISA detection kit. A PDCoV S protein is expressed by adopting an ExpiCHO expression system, then the purified S protein is used as an immunogen to immunize a mouse, and the monoclonal antibody is obtained. And then, respectively using the monoclonal antibody 3D7 and the MAb 9G4 labeled by the HRP as capture and detection antibodies, and establishing the double-antibody sandwich ELISA kit. The kit has good sensitivity, the detection limit of purified S protein is 0.12 ng / mL, and the detection limit of PDCoV is 1.96 * 10 < 3 > copies / [mu] L. Specificity analysis shows that the kit has no cross reactivity with other porcine intestinal coronaviruses PEDV, TGEV and PoRV with clinical symptoms similar to PDCoV, the consistency with an RT-PCR (reverse transcription-polymerase chain reaction) method reaches 91.03%, and the Kappa value is 0.814, so that the reliability of the kit for clinical sample detection is shown. In addition, the kit can also quantitatively evaluate the content of S protein in PDCoV inactivated vaccines and subunit vaccines, and provides a powerful tool for evaluating the vaccines.

The invention belongs to the technical field of biology, and particularly relates to a CHO cell suspension culture medium as well as a preparation method and application thereof. The culture medium comprises amino acids, vitamins, inorganic salts, trace elements, carbohydrates and other molecular compounds; the amino acids comprise glycine, L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cysteine, L-cystine, L-glutamic acid, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine. The culture medium provided by the invention can meet the high-density suspension culture of various CHO cells, can improve the production efficiency and reduce the production cost, and has wide application prospects and values. The culture medium provided by the invention overcomes the defects of the existing culture medium with determined chemical components, and provides more choices for the culture medium with determined chemical components in China.

The invention discloses a cysteine fluorescent probe capable of lasting imaging and a preparation method and application thereof, the structure of the probe is one of the following formulas 1-2, and the probe has the functions of detecting the level of cysteine (Cys) in cells and organisms and lasting imaging. The cysteine fluorescent probe capable of lasting imaging is a novel Cys small-molecule fluorescent probe, and compared with the previously reported Cys small-molecule fluorescent probe, the cysteine fluorescent probe capable of lasting imaging has the advantages that the content of Cys in cells and living bodies can be monitored for a long time; the probe has a wide application prospect in early clinical diagnosis of diseases and long-term imaging of living bodies based on small molecular probes in the future. # imgabs0 #

The invention discloses a method for preparing vinyl sulfate by continuously oxidizing vinyl sulfite. In a fixed bed reactor, vinyl sulfite is used as a raw material and hydrogen peroxide is used as an oxidant. Under the condition of continuous introduction of carrier gas at 5-100°C, a high conversion rate of 98% of the raw material and a product selectivity of 88% are achieved. The catalyst is not deactivated after 300 hours of reaction and has good stability. The invention removes the reaction product in time and reduces product hydrolysis by continuously introducing carrier gas into the fixed bed reactor, thereby improving product selectivity. The fixed bed reactor is adopted to solve the problems of complex operation of intermittent reactors, difficult catalyst separation, discontinuous reaction, etc. The method for continuously preparing vinyl sulfate provided by the invention is simple, efficient, and has low energy consumption. The cheap titanium silicon molecular sieve reduces the reaction cost and is easy to industrialize, providing a new route for the efficient and green conversion of vinyl sulfite to prepare vinyl sulfate.

The invention is suitable for the technical field of organic chemical synthesis, and provides a novel condensed ring group macrocyclic aromatic hydrocarbon and a synthesis method thereof. The fused-ring-based macrocyclic aromatic hydrocarbon has the advantages of simple synthesis, easy purification and high synthesis yield, and different fused rings are hybridized in the same macrocyclic aromatic hydrocarbon skeleton at high yield through classic Friedel-Crafts acylation and Friedel-Crafts alkylation reactions. The condensed ring group macrocyclic aromatic hydrocarbon with diversified structures, good host-guest properties and high quantum yield luminescence is successfully obtained. In addition, the novel condensed ring group macrocyclic aromatic hydrocarbon and an electron-deficient object are co-crystallized in a solution or ground in a solid state to prepare a corresponding light-emitting charge transfer assembly, a new construction element is provided for developing supramolecular charge transfer co-crystals, and development of the field is promoted.

The invention discloses a preparation method of a chicken DKK1 protein polyclonal antibody, and relates to the technical field of poultry genetic engineering, and the method comprises the following steps: (1) constructing a chicken DKK1 gene recombinant expression plasmid by using nucleotide as shown in SEQ ID No.1 and a prokaryotic expression vector; (2) transforming the chicken DKK1 gene recombinant expression plasmid into a host cell, then performing screening and induced expression to obtain a His-DKK1 fusion protein, and purifying the His-DKK1 fusion protein; and (3) immunizing an animal with the purified His-DKK1 fusion protein, and performing serum separation and purification to obtain the chicken DKK1 protein polyclonal antibody. The invention also provides an application of the chicken DKK1 protein polyclonal antibody in a chicken DKK1 protein detection reagent or kit. The DKK1 protein in chicken tissues in different development stages can be specifically recognized, and an important tool is provided for functional research of DKK1 on chickens.

The invention provides a zinc-based metal organic framework material as well as a preparation method and application thereof. The zinc-based metal organic framework material comprises a mixed coordination framework which is formed by coordination of N and O and has pcu topology, and the minimum asymmetric unit of the zinc-based metal organic framework material is composed of three zinc ions, three deprotonated 1H-indazole-5-carboxylic acids and bridging water molecules. The preparation method comprises the following steps: heating a zinc salt aqueous solution to a reaction temperature, keeping for 30-40 minutes, cooling, and filtering for later use; dissolving an organic ligand in an organic solvent to obtain a ligand solution, heating to a reaction temperature, keeping for 30-40 minutes, and filtering for later use; mixing the standby solutions obtained by respective filtration, removing impurities, and adding a regulator to obtain a mixed solution; and carrying out standing reaction and purification treatment. The zinc-based metal organic framework material provided by the invention is a porous material formed by coordination self-assembly of an organic ligand and zinc ions, has a large specific surface area and aromatic ring modified pore walls, is more beneficial to adsorption of propylene and propane and weakening of adsorption of other low-carbon hydrocarbons, and is high in separation selectivity.