161results about How to "Easy to purify" patented technology

The invention relates to the technical field of bioengineering, and discloses a mutant of Burkholderia lipase as well as a recombinant expression vector and application of the mutant, the mutant comprises P131S, T132A, T251A, T251S, P131A, P131Q, T132G, Q88S and P113A single processes; and a Q88S double process and a P113A double process; and a multi-site mutation combination: a combination I: Q88S, P113A, T132G and L287S, and a combination II: Q88S, P113A, T132G and L287S; a combination II: Q88S, P113A, P131S and L287S; and a combination III: Q88S, P113A, P131S and T251A. Based on experiments, the invention finds that each mutant can not only improve the protein yield, but also improve the enzyme activity by 57% at most; ePA enrichment experiments find that the EPA content of the mutant can be increased by 33% at most, and the mutant has excellent EPA purification and enrichment effects.

The invention discloses a rare-earth metal arylamine group compound as well as preparation method and application thereof. The invention discloses the rare-earth metal arylamine group compound which is characterized in that a general formula of the rare-earth metal arylamine group compound is (2,6-R12PhNH)5LnLi2(THF)2, wherein Ln is a rear-earth metal selected from one of neodymium, samarium, ytterbium or yttrium; R1 is selected from one of hydrogen, methyl or isopropyl. The rare-earth metal arylamine group compound has the advantages that the rare-earth metal arylamine group compound has a definite structure; raw materials used in the preparation method are simple, easily obtained and cheap; the reaction process is simple and easily operated; the rare-earth metal arylamine group compound product is conveniently purified and the yield of the product is high; the rare-earth metal arylamine group compound has a high activity when acting as a single component catalyst to catalyze a hydrogen phosphine reaction of aldehyde or ketone and phosphite ester; the use amount of the catalyst is less; the yield is high; a substrate has wide universality; a new and simple method is provided for synthesis of a-hydroxyphosphonate.

The invention relates to the technical field of chemical synthesis, and particularly provides a preparation method of a semaglutide side chain, which comprises the following steps: step S1, carrying out reaction on octadecanedioic acid mono-tert-butyl ester and trifluoroacetic acid pentafluorophenyl ester to generate a first intermediate; s2, enabling the first intermediate to react with 1-tert-butyl L-glutamic acid to generate a second intermediate; s3, enabling the second intermediate to react with pentafluorophenyl trifluoroacetate to generate a third intermediate; and S4, carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid, so as to generate a semaglutide side chain, and carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid. According to the preparation method disclosed by the embodiment of the invention, the pentafluorophenyl trifluoroacetate is used as the condensation reagent for two times to activate the carboxylic acid, and the amidation reaction is carried out for two times to construct the amido bond after each activation, so that compared with the traditional carbodiimide and urea positive ion type condensation reagent, the chiral racemization in the condensation process can be effectively avoided, and the yield of the product is improved. The condensation chiral racemization impurity can be lower than 0.3%.

The invention provides a synthesis method of 6-(t-butyloxycarboryl)-3, 6-diazabicyclo [3.1. 1] heptane, which comprises the following steps: starting from N-benzyl-2, 6-piperidinedione, firstly forming a dibromide 1, then forming a bridge ring intermediate 2 under alkaline conditions, then removing benzyl to obtain an intermediate 3, then reducing dicarbonyl to form free amine 4, and finally synthesizing the 6-(t-butyloxycarboryl)-3, 6-diazabicyclo [3.1. 1] heptane. Then performing Boc protection on amino to obtain an intermediate 5, and performing debenzylation to form a target product. According to the synthesis method, few impurities are generated, and the product is easy to purify and high in purity, so that the total yield is increased, the unit cost is effectively reduced, and large-scale industrial production is facilitated.

An in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply relates to the field of solid waste resource utilization and renewable energy technology. The purpose of the present invention is to solve the problems of low hydrogen solubility and insufficient carbon dioxide bioconversion in the biogas purification process, as well as the inhibitory effect of excessive hydrogen dosage on biogas production by anaerobic digestion of sludge. The present invention utilizes the anaerobic digestion process of sludge in an anaerobic environment to produce biogas, and adds hydrogen to the process by direct hydrogen input or zero-valent iron corrosion, so that hydrogen penetrates into the liquid phase. The hydrogenotrophic methanogens in the system use hydrogen as an electron donor and carbon dioxide in the biogas produced by anaerobic digestion of sludge as an electron acceptor to convert hydrogen and carbon dioxide into methane to achieve in-situ biogas purification. The present invention can obtain an in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply.

The invention provides a preparation method of cresol trozole trisiloxane, and belongs to the technical field of sun-screening agent preparation. The preparation method of cresol trozole trisiloxane comprises the following steps: carrying out Claisen rearrangement reaction on a concentrated solution of a compound as shown in a formula II to obtain a compound as shown in a formula III; and reacting the compound shown in the formula III with heptamethyltrisiloxane to obtain a compound shown in a formula IV. The concentrated solution of the compound shown in the formula II is subjected to the Claisen rearrangement reaction under the solvent-free condition to prepare the compound shown in the formula III, the reaction time for preparing the compound shown in the formula III is shortened, the reaction efficiency for preparing the compound shown in the formula III is improved, the yield for preparing the compound shown in the formula III is increased, the reaction time for preparing cresol trozole trisiloxane is shortened, and the yield of cresol trozole trisiloxane is increased. The reaction efficiency of cresol trozole trisiloxane preparation is improved, and the yield of cresol trozole trisiloxane preparation is improved.

The invention provides a preparation method of high-purity molybdenum trioxide, which belongs to the technical field of molybdenum trioxide production and comprises the following steps: S1, crushing, grinding and roasting molybdenum ore sand to obtain crude molybdenum oxide, adding the crude molybdenum oxide into a sodium hydroxide solution, heating, stirring and filtering to obtain a crude sodium molybdate solution; s2, adjusting the pH value of the crude sodium molybdate solution to 5.5-7.0, introducing the crude sodium molybdate solution into an exchange column of macroporous cation exchange resin, then adjusting the pH value of the solution to 7.0-8.5, and introducing the crude sodium molybdate solution into an exchange column filled with anion exchange resin to obtain a high-purity sodium molybdate solution; s3, excessive ammonia water is added into the high-purity sodium molybdate solution, ammonium molybdate is separated out, washing and drying are conducted, and high-purity ammonium molybdate is obtained; and S4, the high-purity ammonium molybdate is put into a rotary calcining furnace, rare earth oxide is sprayed on the inner wall of a furnace tube, and the high-purity molybdenum trioxide is prepared by adopting a calcining mode of firstly heating, then cooling and then heating. According to the invention, the purpose of producing high-purity molybdenum trioxide is realized.

The invention relates to the field of separation and purification, and discloses a norbornene separation and purification method and system. The method provided by the invention is suitable for separation and purification of the norbornene synthesis liquid obtained by a solvent method, high-purity norbornene can be obtained in two steps, and meanwhile, a solvent which can be reused in a norbornene synthesis process can be directly obtained. According to the method, high-purity norbornene suitable for COC synthesis can be obtained at a high yield, and the method has the advantages of being short in technological process, high in resource utilization rate, reliable in process, easy to manage, easy to industrially popularize and the like.

The invention discloses a porcine delta-coronavirus double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) detection kit based on an S protein monoclonal antibody as well as a use method and application of the porcine delta-coronavirus double-antibody sandwich ELISA detection kit. A PDCoV S protein is expressed by adopting an ExpiCHO expression system, then the purified S protein is used as an immunogen to immunize a mouse, and the monoclonal antibody is obtained. And then, respectively using the monoclonal antibody 3D7 and the MAb 9G4 labeled by the HRP as capture and detection antibodies, and establishing the double-antibody sandwich ELISA kit. The kit has good sensitivity, the detection limit of purified S protein is 0.12 ng / mL, and the detection limit of PDCoV is 1.96 * 10 < 3 > copies / [mu] L. Specificity analysis shows that the kit has no cross reactivity with other porcine intestinal coronaviruses PEDV, TGEV and PoRV with clinical symptoms similar to PDCoV, the consistency with an RT-PCR (reverse transcription-polymerase chain reaction) method reaches 91.03%, and the Kappa value is 0.814, so that the reliability of the kit for clinical sample detection is shown. In addition, the kit can also quantitatively evaluate the content of S protein in PDCoV inactivated vaccines and subunit vaccines, and provides a powerful tool for evaluating the vaccines.

The invention belongs to the technical field of biology, and particularly relates to a CHO cell suspension culture medium as well as a preparation method and application thereof. The culture medium comprises amino acids, vitamins, inorganic salts, trace elements, carbohydrates and other molecular compounds; the amino acids comprise glycine, L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cysteine, L-cystine, L-glutamic acid, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine. The culture medium provided by the invention can meet the high-density suspension culture of various CHO cells, can improve the production efficiency and reduce the production cost, and has wide application prospects and values. The culture medium provided by the invention overcomes the defects of the existing culture medium with determined chemical components, and provides more choices for the culture medium with determined chemical components in China.

The invention discloses a preparation method of a chicken DKK1 protein polyclonal antibody, and relates to the technical field of poultry genetic engineering, and the method comprises the following steps: (1) constructing a chicken DKK1 gene recombinant expression plasmid by using nucleotide as shown in SEQ ID No.1 and a prokaryotic expression vector; (2) transforming the chicken DKK1 gene recombinant expression plasmid into a host cell, then performing screening and induced expression to obtain a His-DKK1 fusion protein, and purifying the His-DKK1 fusion protein; and (3) immunizing an animal with the purified His-DKK1 fusion protein, and performing serum separation and purification to obtain the chicken DKK1 protein polyclonal antibody. The invention also provides an application of the chicken DKK1 protein polyclonal antibody in a chicken DKK1 protein detection reagent or kit. The DKK1 protein in chicken tissues in different development stages can be specifically recognized, and an important tool is provided for functional research of DKK1 on chickens.

The invention provides a method for synthesizing semaglutide, wherein Fmoc-N(FMPB)-Gly-OtBu and HBTU react with a swollen AM Resin in the presence of an activator to obtain Fmoc-N(FMPB-AM Resin)-Gly-OtBu; Fmoc-Arg(Pbf)-OH and HOBT are added to a reaction system of a resin and a PIP / DMF solution, and reacted under DIC activation conditions until the ninhydrin test is negative; the above steps of removing Fmoc protection and coupling the corresponding amino acid or polypeptide fragment are repeated, and the peptide resin coupling is completed sequentially from the C-terminus to the N-terminus according to the semaglutide sequence, and the crude semaglutide is obtained by cleavage, filtration, precipitation, and drying. The method adopts the condensation of the modified Gly with the amino resin to obtain the resin, which can be successfully completed at a higher temperature, and the synthesis efficiency and synthesis yield are higher.

The invention provides a signal peptide for enhancing cell-free system protein expression and application of the signal peptide in D2P, and particularly, by optimizing a signal peptide sequence, enhancing the yield of cell-free expression protein and saving time cost and capital cost, commercial application of protein in the fields of scientific research, medicine, massive health, beauty, food and the like is greatly promoted.

The utility model discloses a device capable of improving the high purity of quartz sand. The device comprises a screening box, a feeding port is formed in the top of the screening box, a plurality of supporting legs are fixedly connected to the bottom of the side wall of the screening box, an impurity removal cavity and an adsorption cavity are formed in the screening box, and the device further comprises a separation screening assembly located in the middle of the screening box; the material beating mechanism is located at the top of the separating and screening assembly; the magnetic suction impurity removal mechanism is located in the middle of the separating and screening assembly and used for adsorbing magnetic minerals in the filtered quartz sand; according to the scheme, by arranging the magnetic suction impurity removal mechanism, tiny magnetic minerals in quartz sand can be circularly adsorbed and removed, so that the situation that the purity of the quartz sand is affected due to the fact that the tiny magnetic minerals are difficult to filter and remove is avoided, and the purpose of effectively improving the purification effect on the quartz sand is achieved; impurities adhered to the quartz sand can be slapped and dispersed, so that the purification of the quartz sand is facilitated.

The invention relates to a wild jujube CYP450 enzyme gene ZjCYP30487 and an application of the wild jujube CYP450 enzyme gene ZjCYP30487 in biological synthesis of jujuboside. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The wild jujube CYP450 enzyme gene ZjCYP30487 provided by the invention can be used for biosynthesis of 16-hydroxyl dammarendiol in a biological synthesis route of jujuboside.

The invention belongs to the field of virus purification, and particularly relates to a foot-and-mouth disease virus plaque purification method and a foot-and-mouth disease virus thereof. The high-quality foot-and-mouth disease virus is obtained by screening and purifying the foot-and-mouth disease virus through a specific 1 * cell culture medium, a 2 * cell culture medium, a cell maintenance medium and a culture solution which are prepared by the method and through a virus plaque purification technology. The seed virus screening and purifying method is low in cost, the problems that an existing seed virus screening method is high in cost, complex in operation and the like are solved, and high-quality foot and mouth disease viruses can be obtained through virus plaque purification. According to the method, the virus titer of the conventional foot-and-mouth disease virus seed virus is increased by more than 3 times, and the content of antigen 146S is increased by 40%.

The invention relates to a method for preparing an electrical equipment insulating liquid from waste edible oil methyl ester with an improved pour ignition point characteristic, and belongs to the technical field of insulating liquids. Distilling the waste edible oil methyl ester to obtain distilled waste edible oil methyl ester; the method comprises the following steps: adding a molecular sieve, immobilized lipase IM-100, neopentyl glycol, distilled waste edible oil methyl ester and methyl nonanoate into a reaction vessel, reacting for 72-96 hours under the conditions that the temperature is 40-50 DEG C and the stirring rotating speed is 120-130 rpm, and separating out the molecular sieve and the immobilized lipase IM-100 to obtain waste edible oil-nonanoate neopentyl glycol diester; and carrying out adsorption, vacuum drying and filter membrane filtration on the obtained waste edible oil-nonanoic acid-based neopentyl glycol diester to generate the waste edible oil-nonanoic acid-based neopentyl glycol diester insulating liquid. The obtained waste edible oil-nonanoic acid neopentyl glycol diester insulating liquid has an excellent low-temperature flow characteristic, and the ignition point also reaches the fireproof safety standard of K-grade insulating oil, so that the risk of accidents of power equipment such as a transformer and the like under severe environment conditions is reduced to a great extent; the vacancy of the insulating liquid in high-safety application places such as offshore wind power and the like is supplemented, and more choices are provided for the application of the insulating liquid.

This invention discloses a recombinant vector, recombinant bacteria, and fermentation method for high-yield GHK production, belonging to the field of biomedical technology. The recombinant expression vector of this application comprises a bicistronic expression cassette controlled by a T7 / lac heterozygous promoter, sequentially containing the encoding (GHK). n The vector encodes the first cistron of the polypeptide and the second cistron, where n is an integer from 2 to 200. When this vector is transformed into *E. coli* BL21, the resulting recombinant bacteria can simultaneously express (GHK) after IPTG induction. n Polypeptides and trypsinogen. During fermentation, trypsinogen is activated and enables the processing of (GHK). n In situ enzymatic cleavage of peptides efficiently releases GHK. This invention integrates the traditional multi-step process into a "one-step fermentation," simplifying the production process and reducing purification difficulty. Examples show that the GHK yield can reach up to 3.9 g / L, providing an efficient and convenient new strategy for the large-scale biomanufacturing of GHK.

The application discloses a kind of tetraphenyl ethylene-chalcone derivative preparation and its application preparation and its application.The structure is as follows: the method includes: 4-(1,2,2-triphenyl ethenyl) acetophenone, aldehyde derivative, sodium hydroxide (provides alkaline condition, pH=8.3~8.5), is placed in anhydrous ethanol, under the condition that heating temperature is 80 DEG C, reflux reaction 12h, after reaction is ended, the functional organic material is separated and purified.The functional organic material synthesized according to the above method has larger conjugated system, and has significant pressure-induced color change characteristics.The functional organic material disclosed in the application has low preparation cost, simple operation, simple method, and the material shows significant pressure-induced color change characteristics, can be applied to pressure-induced color change, luminescent material, printing and dyeing, color printing and anti-counterfeiting technical field.

The invention provides a polyunsaturated sulfobetaine containing a glycidyl group, which is used for graft crosslinking modification of starch, cellulose, polyurethane, polyamide, polyester, polyolefin or polyether etc. containing amino or hydroxyl groups in the polymer chain structure, and simultaneously introduces quaternary ammonium cations and unsaturated sulfobetaine to give the modified polymer material hydrophilicity, antibacterial property, polymerizability and the like.

The invention belongs to the technical field of bioengineering, and particularly relates to a DKK3 nano antibody, a detection kit and application of the DKK3 nano antibody. The nucleotide sequence and the amino acid sequence of the DKK3 nano antibody provided by the invention are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2. The molecular weight of the DKK3 nano antibody is about 16 kD, and the DKK3 nano antibody can be specifically combined with DKK3 protein, has relatively high affinity, can be used for accurately detecting expression of DKK3 in tissues by an immunohistochemical method, and provides a new method for research on DKK3 gene functions.

The invention belongs to the field of medicinal chemistry, and particularly relates to a colopivir synthesis process which is simple in process route, cheap and safe in reagent, free of heating and cooling operation, low in energy consumption, good in product quality, high in yield and wide in commercial development prospect, the synthesis efficiency and operation convenience are greatly improved, and the time cost is reduced.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention discloses benzaldehyde p-toluenesulfonyl hydrazone and a synthesis method thereof, and belongs to the technical field of organic compounds. The preparation method comprises the following steps: mixing benzaldehyde, p-toluenesulfonyl hydrazide and water, and carrying out condensation reaction to obtain benzaldehyde p-toluenesulfonyl hydrazone. According to the method, green and environment-friendly water is adopted as a solvent, the reaction temperature is mild, purification is easy, the requirement for equipment is low, the purity of the obtained benzaldehyde p-toluenesulfonyl hydrazone is larger than 99.5%, and the yield reaches up to 99.9% or above.

The invention discloses a recombinant vector containing a human astrovirus nucleic acid fragment, which is obtained by inserting an exogenous nucleotide sequence into a prokaryotic expression vector skeleton, and the exogenous nucleotide sequence is formed by sequentially connecting the following gene sequences: an SUMO fusion tag DNA sequence, an SAP gene sequence, an MS2 phage related DNA sequence and a human astrovirus sequence fragment. The tail end of the prepared human astrovirus pseudovirus particle contains a stem-loop structure, so that the human astrovirus pseudovirus particle is not easily degraded by nuclease, and the stability of virus nucleic acid RNA (Ribonucleic Acid) is improved; meanwhile, a human astrovirus conservative RNA gene sequence is wrapped by the phage MS2 coat protein, so that the phage MS2 coat protein has relatively high ribonuclease and deoxyribonuclease resistance, and the long-term stability of virus nucleic acid RNA is enhanced. And the stability problem of the virus RNA quality control product in the production and use processes is solved.

The invention relates to a method for preparing a hydroxyl naphthaldehyde derivative by using a cobalt complex, which comprises the following step: by taking naphthol and paraformaldehyde as raw materials and the cobalt complex containing an ortho-carborane benzothiazole structure as a catalyst, reacting at room temperature to synthesize the hydroxyl naphthaldehyde derivative. Compared with the prior art, the cobalt complex containing the ortho-carborane benzothiazole structure is applied to the reaction for catalytically synthesizing the hydroxyl naphthaldehyde derivative through the one-pot method, the use equivalent of the catalyst is low, the reaction condition is mild, the substrate universality is high, and the yield is high.

The invention discloses an organic compound, a mixture, a composition, an organic light-emitting device and a display panel. The organic compound has a structure as shown in a general formula (1) or a general formula (2): # imgabs0 # imgabs1 #. The organic compound has better overall molecular solubility and is easy to purify, so that the purity of the organic compound is improved, and the efficiency and the service life of an organic light-emitting device prepared from the organic compound are further improved.

The invention discloses a PEDV-S1 recombinant protein antigen as well as a preparation method and a vaccine thereof, and belongs to the technical field of recombinant protein vaccines. The CHO eukaryotic expression system is adopted, high expression and complete glycosylation modification of protein can be achieved, and therefore it is guaranteed that the vaccine has strong immunogenicity; through His tag fusion expression design, nickel column affinity chromatography is conveniently adopted for purification, the process is simple and convenient, and the protein recovery rate is also improved; in combination with a serum-free suspension culture technology, the method is suitable for large-scale production, and the stability among batches is good; the prepared vaccine is high in safety and few in side reaction; an immune challenge test further proves that the vaccine can induce a body to generate a high-level antibody and can effectively protect PEDV attack.

A cyclohexyl imine production system comprises a reactor, a filter, a dealcoholization tower, a decanter, and a separation tower, wherein a feed port is provided at the bottom of the reactor for connecting with adiponitrile, ethanol, alkali, and a hydrogen source, a discharge port is provided at the top of the reactor, and the dealcoholization tower is fed through the filter, the bottom liquid outlet of the dealcoholization tower is connected to the decanter, and the light phase outlet of the decanter is connected to the feed port of the separation tower. The present invention has a simple structure and is easy to operate, and can continuously and stably produce cyclohexyl imine and hexamethylenediamine at the same time, meeting the actual needs of chemical enterprises.

The invention relates to a method for catalytically synthesizing 2, 4-disubstituted phthalazine 1-(2H)-ketone from 1, 5, 7-triazabicyclo [4.4. 0] dec-5-ene. According to the method, 3-alkenyl phthalide and hydrazine compounds are taken as initial raw materials, TBD which is low in price and easy to obtain is taken as a catalyst, sodium tert-butoxide is taken as alkali, reaction is carried out at room temperature, and the 2, 4-disubstituted phthalazine 1-(2H)-ketone is obtained at high yield. According to the method, the cheap TBD is adopted as the catalyst, the reaction is carried out under the room temperature condition, and compared with a traditional reaction strategy, the economic cost is greatly reduced, the steps are simple, heating and a transition metal catalyst are not needed, operation is convenient, the yield is high, the cost is low, and good economic benefits are achieved.