88results about How to "Easy to purify" patented technology

The invention discloses a rare-earth metal arylamine group compound as well as preparation method and application thereof. The invention discloses the rare-earth metal arylamine group compound which is characterized in that a general formula of the rare-earth metal arylamine group compound is (2,6-R12PhNH)5LnLi2(THF)2, wherein Ln is a rear-earth metal selected from one of neodymium, samarium, ytterbium or yttrium; R1 is selected from one of hydrogen, methyl or isopropyl. The rare-earth metal arylamine group compound has the advantages that the rare-earth metal arylamine group compound has a definite structure; raw materials used in the preparation method are simple, easily obtained and cheap; the reaction process is simple and easily operated; the rare-earth metal arylamine group compound product is conveniently purified and the yield of the product is high; the rare-earth metal arylamine group compound has a high activity when acting as a single component catalyst to catalyze a hydrogen phosphine reaction of aldehyde or ketone and phosphite ester; the use amount of the catalyst is less; the yield is high; a substrate has wide universality; a new and simple method is provided for synthesis of a-hydroxyphosphonate.

The invention relates to a wild jujube CYP450 enzyme gene ZjCYP30487 and an application of the wild jujube CYP450 enzyme gene ZjCYP30487 in biological synthesis of jujuboside. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The wild jujube CYP450 enzyme gene ZjCYP30487 provided by the invention can be used for biosynthesis of 16-hydroxyl dammarendiol in a biological synthesis route of jujuboside.

This invention discloses a recombinant vector, recombinant bacteria, and fermentation method for high-yield GHK production, belonging to the field of biomedical technology. The recombinant expression vector of this application comprises a bicistronic expression cassette controlled by a T7 / lac heterozygous promoter, sequentially containing the encoding (GHK). n The vector encodes the first cistron of the polypeptide and the second cistron, where n is an integer from 2 to 200. When this vector is transformed into *E. coli* BL21, the resulting recombinant bacteria can simultaneously express (GHK) after IPTG induction. n Polypeptides and trypsinogen. During fermentation, trypsinogen is activated and enables the processing of (GHK). n In situ enzymatic cleavage of peptides efficiently releases GHK. This invention integrates the traditional multi-step process into a "one-step fermentation," simplifying the production process and reducing purification difficulty. Examples show that the GHK yield can reach up to 3.9 g / L, providing an efficient and convenient new strategy for the large-scale biomanufacturing of GHK.

The application discloses a kind of tetraphenyl ethylene-chalcone derivative preparation and its application preparation and its application.The structure is as follows: the method includes: 4-(1,2,2-triphenyl ethenyl) acetophenone, aldehyde derivative, sodium hydroxide (provides alkaline condition, pH=8.3~8.5), is placed in anhydrous ethanol, under the condition that heating temperature is 80 DEG C, reflux reaction 12h, after reaction is ended, the functional organic material is separated and purified.The functional organic material synthesized according to the above method has larger conjugated system, and has significant pressure-induced color change characteristics.The functional organic material disclosed in the application has low preparation cost, simple operation, simple method, and the material shows significant pressure-induced color change characteristics, can be applied to pressure-induced color change, luminescent material, printing and dyeing, color printing and anti-counterfeiting technical field.

The invention belongs to the field of medicinal chemistry, and particularly relates to a colopivir synthesis process which is simple in process route, cheap and safe in reagent, free of heating and cooling operation, low in energy consumption, good in product quality, high in yield and wide in commercial development prospect, the synthesis efficiency and operation convenience are greatly improved, and the time cost is reduced.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention relates to a method for preparing a hydroxyl naphthaldehyde derivative by using a cobalt complex, which comprises the following step: by taking naphthol and paraformaldehyde as raw materials and the cobalt complex containing an ortho-carborane benzothiazole structure as a catalyst, reacting at room temperature to synthesize the hydroxyl naphthaldehyde derivative. Compared with the prior art, the cobalt complex containing the ortho-carborane benzothiazole structure is applied to the reaction for catalytically synthesizing the hydroxyl naphthaldehyde derivative through the one-pot method, the use equivalent of the catalyst is low, the reaction condition is mild, the substrate universality is high, and the yield is high.

The invention discloses a PEDV-S1 recombinant protein antigen as well as a preparation method and a vaccine thereof, and belongs to the technical field of recombinant protein vaccines. The CHO eukaryotic expression system is adopted, high expression and complete glycosylation modification of protein can be achieved, and therefore it is guaranteed that the vaccine has strong immunogenicity; through His tag fusion expression design, nickel column affinity chromatography is conveniently adopted for purification, the process is simple and convenient, and the protein recovery rate is also improved; in combination with a serum-free suspension culture technology, the method is suitable for large-scale production, and the stability among batches is good; the prepared vaccine is high in safety and few in side reaction; an immune challenge test further proves that the vaccine can induce a body to generate a high-level antibody and can effectively protect PEDV attack.

The invention relates to a method for catalytically synthesizing 2, 4-disubstituted phthalazine 1-(2H)-ketone from 1, 5, 7-triazabicyclo [4.4. 0] dec-5-ene. According to the method, 3-alkenyl phthalide and hydrazine compounds are taken as initial raw materials, TBD which is low in price and easy to obtain is taken as a catalyst, sodium tert-butoxide is taken as alkali, reaction is carried out at room temperature, and the 2, 4-disubstituted phthalazine 1-(2H)-ketone is obtained at high yield. According to the method, the cheap TBD is adopted as the catalyst, the reaction is carried out under the room temperature condition, and compared with a traditional reaction strategy, the economic cost is greatly reduced, the steps are simple, heating and a transition metal catalyst are not needed, operation is convenient, the yield is high, the cost is low, and good economic benefits are achieved.

The utility model discloses a crushing device for extracting ossein, which relates to the technical field of ossein extracting and crushing appliances and comprises a support and an aggregate collecting mechanism, a feeding hopper is arranged at the top of the support, a roller cavity is formed in the feeding hopper, a crushing mechanism is arranged in the roller cavity of the aggregate collecting mechanism, and a supporting plate is arranged in the middle of the support of the aggregate collecting mechanism. A screening mechanism is arranged at the top of the aggregate collecting mechanism supporting plate, and an aggregate collecting mechanism is correspondingly arranged below the screening mechanism of the aggregate collecting mechanism; according to the aggregate crushing and screening device, aggregate can be efficiently crushed conveniently through cooperative work of the crushing roller and the motor in the crushing mechanism, the crushed aggregate can be screened conveniently through cooperation of the filter plate and the vibration motor in the screening mechanism, and finally the problems that the aggregate which does not meet the granularity requirement is mixed into a qualified product and the material is prone to splashing are solved.

The application relates to the technical field of organic synthesis, in particular to a polymeric Schiff base metal compound, a preparation method and application thereof, the polymeric Schiff base metal compound has structures shown in formula I to formula IV. The polycentered polymeric Schiff base metal compound provided by the application has the advantages of simple synthesis method, easy purification and high product purity. Since long-chain polyether glycol is introduced, the melting point of the Schiff base catalyst is low, and the Schiff base catalyst is better dispersed in glycolide. The polymeric Schiff base catalyst has a polycentered structure, and the catalytic activity of the original single-center catalyst can be improved through the synergistic coordination effect between the poly-metal centers. The polymeric Schiff base catalyst provided by the application has high catalytic activity, and when the polymeric Schiff base catalyst is used to catalyze the ring-opening polymerization of glycolide to generate polyglycolic acid, the polymerization reaction speed is high. Experimental results show that when the polyglycolic acid is prepared by using the catalyst provided by the application, the reaction yield can reach 97.2%.

The application relates to the technical field of light-emitting materials, in particular to an organic compound, a composition and an organic electronic device. The organic compound has a structure as shown in any one of general formula (I) to (III). The organic compound is a novel light-emitting material, and when the organic compound is used in an organic electronic device, the problems of low light-emitting efficiency and short service life of the organic electronic device can be solved.

This invention provides a method for synthesizing N-cyclohexyl-2-benzothiazole, comprising the following steps: reacting cyclohexylamine and 2-halobenzothiazole in an alcohol solvent in the presence of a deacidifying agent at 60-80°C to generate N-cyclohexyl-2-benzothiazole. The method of this invention is simple to operate, uses readily available raw materials, does not use halogen-containing catalysts, has low pollution, and produces products with high purity and yield. It also avoids the use of DMSO as a solvent, thus solving the problem of difficult DMSO recovery and purification.

The application discloses strigasiaticaside A and an extraction method and application thereof, and finds, through chemical component research on the whole grass of striga asiatica, a new synbinaphthyl tetrahydrofuran lignan glycoside, that is, strigasiaticaside A, identifies the chemical structure of the new compound through nuclear magnetic resonance, mass spectrometry, ultraviolet, infrared and circular dichroism spectrum technology.The structure of the new compound is identified as (-) sesamin-5-O-beta-D-glucopyranoside.The anti-hepatitis activity of the new natural product is evaluated by using LPS stimulated HepG2 cells.The results show that when the concentration is 100 muM, the compound has a significant inhibitory effect on the production of inflammatory factors IL-10 and NF-kappa B protein, and has no cytotoxicity, indicating that the compound may have potential anti-hepatitis activity.

The invention relates to the technical field of conjugated microporous polymer materials, and discloses a covalent triazine unit-based conjugated microporous polymer membrane with negative charges and a preparation method thereof. The method comprises the following steps: firstly, preparing a negatively charged cyanophenyl monomer through nucleophilic substitution reaction of a cyanophenyl-containing compound and 1, 4-butane sultone, then taking the monomer and biphenyl nitrile as comonomers, and carrying out step-by-step polymerization, heating film formation, shock cooling shaping and dual-solvent soaking post-treatment under the catalysis of trifluoromethanesulfonic acid to obtain the cyanophenyl-containing polymer film. And finally obtaining the covalent triazine unit-based conjugated microporous polymer membrane with negative charges. The preparation process is high in controllability, and the obtained film has high stability of a covalent triazine structure and the porous characteristic and negative charge function of conjugated micropores, and has wide application prospects in the fields of gas separation and the like through verification.

The present application relates to the field of chemistry, and provide a kind of preparation method of glycyrrhizin dipotassium salt based on chelating resin deep removal heavy metal, comprising the following steps: glycyrrhizin monoammonium salt is added to alkaline pure water solution, stirring, adjust pH to 7.5~8.5, obtain solution;The obtained solution is passed through mixed resin column, flush mixed resin column, combine effluent, concentrate and dry, obtain glycyrrhizin dipotassium salt;Wherein, the mixed resin in the mixed resin is the composition of cation exchange resin and new chelating resin.The preparation method of the present application is simple and reasonable, green and environmental protection, in the preparation process, using cation exchange resin and new chelating resin promote glycyrrhizin dipotassium salt purification, so that the residual alcohol content in the obtained glycyrrhizin dipotassium salt is ≤500mg / kg, residual ammonia content is ≤40mg / kg, and lead, cadmium, arsenic, mercury content is all ≤0.2mg / kg.

The invention discloses an eurytopic chlamydomonas reinhardtii expression vector based on a PSAD promoter and a 3 * Flag tag as well as a construction method and application of the eurytopic chlamydomonas reinhardtii expression vector. The nucleotide sequence of the eurytopic chlamydomonas reinhardtii expression vector is shown as SEQ ID No.1; the vector comprises a PSAD promoter, a target gene insertion site EcoR V, a 3 * Flag tag at the 3'terminal, an rbcS2 terminator, a TUB2 promoter, a hygromycin B screening gene and an rbcS2 terminator. The construction method comprises the following steps: constructing a skeleton of a pHyg-PSAD-3Flag vector; constructing a sequence fragment fused with a 3 * Flag tag; and connecting the sequence fragment fused with the 3 * Flag tag with a plasmid skeleton fragment through T4 DNA (deoxyribonucleic acid) enzyme so as to construct the pHyg-PSAD-3Flag vector. According to the invention, the expression plasmid of the chlamydomonas pHyg-PSAD-3Flag can be used for efficiently screening algae strains capable of stably expressing target protein.

The application discloses a green preparation method of 4,4'-difluorobenzophenone. The method uses 4,4'-difluorobenzene as raw material, potassium bromide as catalyst, acetic acid as solvent, and oxygen as oxidant, and 4,4'-difluorobenzophenone is generated in high selectivity through one-step oxidation under mild conditions. The application surprisingly finds that in the presence of potassium bromide, a new non-metallic catalytic oxidation system of potassium bromide / acetic acid / oxygen is used to realize high-efficiency and high-selectivity conversion of the C-H bond at the benzyl position. Compared with the traditional method, the application does not need to use a transition metal catalyst, does not need to use carbon tetrachloride and other materials damaging the ozone layer, and does not need to involve a dangerous intermediate such as a diazonium salt, the process route is short, the atomic economy is high, the product yield is high, the product purity is high, the catalyst is cheap and easy to obtain, and the application has excellent industrial application prospect and green chemistry characteristics.

The utility model discloses a quenching device for high-strength alloy steel, which relates to the technical field of alloy steel quenching appliances and comprises a quenching tank, a plurality of condenser pipes are mounted at the lower end in the quenching tank, two ends of each condenser pipe penetrate through the quenching tank, a screen box is arranged at the upper end in the quenching tank, and the screen box is abutted against the inner wall of the quenching tank; according to the utility model, the motor is matched with the turntable to control the rotation of the connecting rod, so that the movement of the clamping jaw is convenient to control; the electric push rod is matched with the fixed rod to control the movement of the connecting plate, so that the clamping of the clamping jaw is convenient to control; the condensation pipe is matched with the quenching pool to control the temperature of the quenching pool, so that the alloy quenching is convenient; impurities of liquid in the quenching pool are controlled, alloy purification is facilitated, the physical strength of workers is saved, and the quenching efficiency of the workers is improved.

The application belongs to the technical field of nuclear fuel cycle and nuclear waste liquid treatment. The application reports a kind of carboxyl-substituted o-phenanthroline water-soluble ligand based on selective masking of part of trivalent actinide under high acidity conditions, and under optimal conditions, the separation coefficient of trivalent europium can reach 120 in 1.5M nitric acid; the separation coefficient of trivalent cerium can reach 4.4. The single crystal structure of the complex is a double metal dimer structure, which guarantees the high acidity tolerance and high selectivity of the ligand. The synthesis of the ligand involved in the application is simple, the extraction efficiency is high, and the extraction mode is novel; the related content will greatly promote the design and performance optimization of water-soluble lanthanum, lanthanum, actinium and actinium separation ligand.

The invention discloses a method for synthesizing tetracyclododecene (TCD) with high selectivity and low byproduct, which comprises the following steps of: pyrolyzing dicyclopentadiene (DCPD) serving as an initial raw material to generate cyclopentadiene (CPD), and reacting with norbornene (NB) in a system to generate TCD. According to the method, the feeding molar ratio of NB to DCPD is controlled to be 1: (0.237-2.138), preferably 3: 1, the reaction is carried out at the temperature of 160-280 DEG C and under the pressure of 3.0-8.0 MPa, CPD auto-agglutination and side reactions of CPD, DCPD, TCD and the like can be effectively inhibited, and the reaction selectivity is remarkably improved. The method is simple in process, the content of by-products in the obtained crude product is low, the components are relatively simple, the solvent and unreacted monomers can be conveniently recovered through rectification, the TCD product with the purity of 95% or above is finally obtained, and the whole process is easy for large-scale production.

The present application relates to a kind of enzyme immobilized chitosan emulsification material and its application in catalytic esterification reaction synthesis phytosterol acetate.The technical scheme used is: lipase is immobilized to chitosan surface by crosslinking agent to prepare the chitosan particle (CSPPL) of enzyme immobilized lipase, then the prepared CSPPL is used to prepare W / O Pickering emulsion catalytic system with phytosterol (PS), and the catalytic system can be used for the synthesis of phytosterol acetate, and each drop of emulsion is used as microreactor of reactant in oil phase.CSPPL can play the role of stabilizer, and also can play the role of catalyst;PS can play the role of stabilizer, and also as reaction substrate participates in catalytic reaction.Compared with homogeneous reaction, phytosterol / chitosan composite particle stable W / O Pickering emulsion catalytic system has good conversion rate.

The invention discloses an asiatic acid saponin compound and medical application thereof. The asiatic acid saponin compound comprises a compound with a structural formula as shown in a formula (I), and pharmaceutically acceptable salt or ester or solvate of the compound. The compound shown in the formula (I) has anti-inflammatory and anti-ulcer activity, so that the compound can be used for preparing medicines for preventing or treating inflammatory diseases or ulcerative diseases.

The invention relates to a formate dehydrogenase mutant and application thereof, and belongs to the field of enzyme engineering and carbon dioxide reduction. The invention provides a formate dehydrogenase mutant. The formate dehydrogenase mutant comprises a formate dehydrogenase catalytic subunit FdhA, an electron transport subunit FdhB and a membrane anchoring protein subunit FdhC, compared with formate dehydrogenase, the formate dehydrogenase mutant has the advantages that a membrane anchor protein subunit FdhC is deleted, or the membrane anchor protein subunit FdhC and an electron transport subunit FdhB are deleted. Compared with the existing formate dehydrogenase, the formate dehydrogenase mutant provided by the invention has high activity and can tolerate oxygen at the same time. The formate dehydrogenase mutant provided by the invention shows huge potential value in the carbon dioxide immobilization industry.

The application relates to the technical field of medicines, and particularly provides a preparation method of lecomycin based on a biosynthesis process. The application first provides a 4''-ketone erythromycin A compound and a recombinant host cell capable of efficiently fermenting the compound. A brand-new semi-chemical synthesis route of lecomycin is designed by taking the key intermediate as a raw material, and the route comprises, in sequence, epoxidation, amination, 6-position oximation, configuration inversion, rearrangement, reduction and ring expansion. The application completely avoids the cumbersome hydroxyl and amino protection and deprotection steps in the prior art, avoids the use of noble metal catalysts, thereby significantly simplifies the process, improves the atomic economy, and the total yield can reach about 32.1%. The application provides a brand-new green synthesis route of lecomycin, which is simple in steps, high in yield, low in cost and easy to industrialize, and meanwhile protects a plurality of novel intermediates in the route.

The application discloses an esterase mutant with improved thermal stability, and belongs to the technical field of enzyme engineering. Bacillus cereus The wild-type carboxylic acid esterase derived from the genus Pseudomonas is used as an evolution template, and through rational design and directional evolution means, multiple mutants are obtained, which are obviously superior to the wild type in catalytic activity, thermal stability, temperature tolerance and organic solvent tolerance. The mutant can adapt to more severe industrial reaction conditions, and has stronger application advantages in industrial production.

The invention discloses heat-resistant glucose dehydrogenase and application thereof in an enzyme fuel cell, and belongs to the crossing field of bioelectrochemistry and new energy technologies. According to the invention, firstly, a heat-resistant glucose dehydrogenase TeGDH is screened, and the heat-resistant glucose dehydrogenase TeGDH is subjected to heterologous expression in escherichia coli BL21 (DE3) and shows good heat stability and catalytic activity; teGDH is dissolved in an anolyte containing an electron mediator anthraquinone-2, 6-disulfonic acid disodium salt, and the heat-resistant high-energy enzyme fuel cell is prepared by adopting a non-immobilized system; the maximum power density of the battery at 50 DEG C reaches 1.74 mW / cm, and the battery has good thermal stability and long-term operation performance; the invention combines big data mining, enzyme engineering and electrochemical technologies, provides a novel enzyme fuel cell solution with high activity, high stability and low cost, and has a good application prospect.

This invention relates to mupirocin compounds, specifically to a method for preparing mupirocin derivatives. The method includes the following steps: 1) benzyloxyheptenol reacts with triethyl orthoacetate via a Claisen rearrangement to generate trans-ethyl olefin ester; 2) the trans-ethyl olefin ester undergoes oxidation to remove debenzylic protection, producing an alcohol compound; 3) the alcohol compound undergoes halogenation to generate a key branched iodide; 4) mupirocin reacts with excess trimethyl orthoformate under acidic catalysis for alcohol protection, followed by ester hydrolysis under alkaline conditions to obtain polyol-protected mupirocin acid; 5) the polyol-protected mupirocin acid reacts with the iodide via a nucleophilic substitution reaction to generate a polyalkyl mupirocin ester; 6) the polyalkyl mupirocin ester undergoes alkaline hydrolysis, followed by acid-base adjustment and deprotection to obtain the crude product, which is then neutralized to acid and purified by slurrying with an inorganic base to obtain the target compound. This invention avoids harsh reaction conditions, has readily available raw materials, high yield, simple operation and purification, and can be used for large-scale production of high-quality target compounds.

The invention belongs to the field of honey purification, and particularly relates to a purification device for honey processing. The invention discloses a honey processing purification device which is integrally mounted, is heated in a waterproof manner and does not affect the treatment efficiency. The honey processing purification device comprises a moving platform, a mounting frame, a mounting container, a preheating part, a filtering part, a pump body, a first connecting pipe, a discharging pipe, a second connecting pipe, a low-temperature concentration tank and the like, a mounting container, a pump body, a low-temperature concentration tank, a condensation pipe and a vacuum pump are sequentially mounted at the top of the movable platform from right to left. All mechanisms for honey purification are mounted on the moving platform, the equipment is convenient to move, purification work is facilitated, the preheating part and the filtering part which are integrally mounted up and down reduce the equipment mounting occupied area, and operation is facilitated; the air pressure in the low-temperature concentration tank is reduced to 20-30 kpa through the vacuum pump, so that the honey can be boiled at the temperature of 45 DEG C or below, and the evaporation speed of the honey water is moderate at the temperature of 45 DEG C.

The application provides a method for asymmetrically catalyzing synthesis of tetra-deuterated 1-(N-methyl imidazole)-4-nitro-3-phenyl butan-1-one derivative. The asymmetric Michael reaction of 1-(N-methyl imidazole)-3-phenyl propyl-2-en-1-one and nitromethane is catalyzed by a chiral ligand-nickel triflate complex, so that 1-(N-methyl imidazole)-4-nitro-3-phenyl butan-1-one derivative is constructed in one step with high yield, high enantioselectivity, high deuterium realization rate and high enantioselectivity, and the substrate has good universality. The product is easy to separate from the catalyst and the raw material; the catalytic system has the advantages of simple operation, no need to exclude air and moisture, convenient product purification and the like, and has great application prospect.