25 results about "Ester hydrolysis" patented technology

The application discloses a Pseudomonas atacamensis STR8 and an application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas atacamensis STR8, and the preservation number is CCTCC NO: M 2025609. The hydrolysis circle HC value of the strain on a tributyrin plate is 2.78, and the lipase activity reaches 139.86 U / mL. The application also provides an application of the strain and a lipase produced by the strain in catalyzing hydrolysis of tocopherol succinate to prepare tocopherol. The strain STR8 is fermented to obtain a crude enzyme liquid, and the crude enzyme liquid is added into a reaction system containing the tocopherol succinate to perform a hydrolysis reaction. After 9 hours of reaction, the content of the tocopherol is increased by 750% compared with a control group. The strain and the method provided by the application have high catalytic efficiency, mild reaction conditions and environmental friendliness, and are suitable for green industrialized production of the tocopherol.

This invention belongs to the field of chemical pharmaceutical technology, and particularly relates to a method for preparing an anti-puncture flash evaporation material. Addressing the issue that the hydrophilicity of the molecular structure in existing technologies needs improvement, this invention provides a maleic methylamine polyethylene glycol activated ester and its preparation method, comprising a reaction condensation of 3-maleimide propionic acid and tert-butyl methylamine polyethylene glycol propionate, followed by acid hydrolysis of the ester, and then adding the activated ester for a catalytic reaction to obtain the final product Mal-CH2CH2COO-N(CH3)-(PEG). n -CH2CH2COONHS. This invention creatively designs a maleic methylamine polyethylene glycol activated lipid structure. The methylamine structure can increase solubility in aqueous media, making it more hydrophilic than the existing Mal-CH2CH2COO-PEG-COONHS molecular structure, which is more conducive to the binding of drugs to the human body environment.

The application discloses a preparation method of 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid. The 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid is prepared from 4-fluoro-2-nitrobenzoic acid methyl ester as a starting material through aromatic nucleophilic substitution reaction, nitro reduction reaction, ester hydrolysis reaction, amino acylation reaction, thioether oxidation reaction and the like. The method has the advantages of mild reaction condition, high yield, low cost, easy availability of raw materials, and the like. The synthesis process meets the requirements of green chemistry, the synthesis route is innovative, the reaction post-treatment is simple, and the product is easy to purify.

The application relates to a chiral triazole-oxazoline compound and a preparation method and application thereof, and belongs to the technical field of organic synthesis. The chiral triazole-oxazoline compound has the structural formula: the preparation method comprises the steps of click reaction, triazole N2 substitution reaction, Suzuki coupling, triazole ester hydrolysis, amidation with a chiral amine alcohol and dehydration condensation. After a chiral triazole-oxazoline compound and a palladium salt compound are added to generate a catalyst in situ, asymmetric addition of aryl boronic acid on a carbon-carbon double bond of a prochiral organic compound is carried out to prepare a chiral organic compound. The application has the advantages of low raw material cost, few synthesis steps, low experiment site requirement, relatively mild reaction conditions and simple operation for preparing the novel triazole-oxazoline compound.

This invention discloses a method for preparing lentilana-degraded impurities. Starting with 3-methyl-5-[5-(3,4,5-trichlorophenyl)-5-trifluoromethyl-4,5-dihydroisoxazol-3-yl]-thiophene-2-carboxylic acid and glycine ester, a condensation reaction is carried out under a condensing agent catalysis, followed by ester hydrolysis under alkaline conditions to obtain lentilana-degraded impurities. This invention features a concise synthetic route, mild reaction conditions, simple operation, and a purity exceeding 96.0%, avoiding the problem of low purification efficiency associated with chromatographic column separation.

This document discloses methods for generating peptides, including liquid-phase peptide synthesis methods in which peptide elongation is carried out in one-pot cycles of one or more. Specifically, this disclosure provides liquid-phase peptide synthesis methods using a solvent system comprising 2-methyltetrahydrofuran for condensation reactions, and aggregation liquid-phase peptide assembly methods in which benzyl alcohol anchor groups are selectively removed from N-protected C-protected peptides via base-catalyzed ester hydrolysis.

This invention relates to the field of thin film technology, specifically providing a polymer thin film and its manufacturing method, comprising the following raw materials in parts by weight: 48-52 parts of recycled polypropylene (rPP), 38-42 parts of polybutylene adipate-butylene terephthalate (PBAT), 7-9 parts of surface-aminated modified nanocellulose whiskers (CNC, diameter 20-30 nm), and 1.2-1.8 parts of bio-based interface stabilizer. This invention utilizes maleic anhydride grafted lignin to form a three-dimensional hydrogen bond network with rPP / PBAT. DSC measurements show a phase separation temperature difference ΔT = 35℃ and an interfacial peel strength of 13.2 N / cm. The synergistic effect of the epoxy-functionalized castor oil ring-opening reaction and the release of reactive oxygen free radicals from mesoporous zinc oxide (6-10 nm) increases the gel content to 72%. Hindered amine additives are released slowly through the mesoporous zinc oxide nanopores, blocking the PBAT ester hydrolysis chain reaction, resulting in a 60% reduction in the measured hydrolysis rate. A temperature path of 155℃→210℃→160℃ is used, with a melt viscosity fluctuation rate of ±2.5%, solving the problem of a narrow processing window caused by the heat sensitivity of PBAT.

A preparation method of 3-iodo-1H-indole-2-carboxylic acid, which comprises the following steps: taking indole-2-carboxylic acid ethyl ester as raw material, converting into 3-formyl-1H-indole-2-carboxylic acid ethyl ester, then converting into 3-iodo-1H-indole-2-carboxylic acid ethyl ester, and finally converting into the target compound 3-iodo-1H-indole-2-carboxylic acid through an ester hydrolysis reaction. The synthesis method realizes the conversion from 3-formyl-1H-indole-2-carboxylic acid ethyl ester to 3-iodo-1H-indole-2-carboxylic acid ethyl ester in a simple method in a key step; finally, the compound 3-iodo-1H-indole-2-carboxylic acid is prepared through simple operation, low cost and relatively mild reaction conditions.

The present application relates to the field of paint technology, in particular to a kind of far infrared paint for floor and preparation method thereof.The paint takes far infrared organic-inorganic hybrid tung oil resin and core-shell far infrared nanoceramic powder as main components, hybrid resin is grafted with tetraethoxysilane and titanium acid tetraisopropyl ester hydrolysis polycondensation after epoxidized tung oil silane, to form Si-O-Si and Si-O-Ti network;Core-shell far infrared nanoceramic powder is coated with polydopamine, branched polyethyleneimine and tannic acid layer.The preparation method includes epoxidation, grafting, polycondensation, core-shell modification and mixing dispersion.The paint has high far infrared emissivity, excellent wear resistance and water resistance, and is storage stable, while retaining natural oil permeability and wood texture.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The application relates to the technical field of fabrics, and discloses a moisture-absorbing, sweat-releasing and antibacterial titanium fiber fabric and a preparation method thereof. The 2,4-bis(N,N-dihydroxyethylamino)-6-[N-(3-aminopropyl)methyl acrylamide]-1,3,5-homotriazine is used to graft modify the polyamide fiber fabric, the hydrophilic amide bond, imino and a large number of hydroxyl groups are grafted to the fabric surface, the hydrophilicity and the moisture regain of the fabric are significantly improved, and the moisture-absorbing and sweat-releasing performance of the fabric is improved. Meanwhile, the limiting oxygen index and the flame-retardant performance are improved. A large number of hydroxyl groups on the surface of the polyamide fabric can interact with the hydroxyl groups on the surface of the titanium dioxide precursor generated by hydrolysis of tetrabutyl titanate, the generated nanometer titanium dioxide can be uniformly loaded on the fabric surface, more photocatalytic antibacterial sites are exposed, and the antibacterial performance of the fabric is significantly improved.

The invention discloses Korean pseudomonas STR7 and application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas koreensis STR7, and the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 2025608. The HC value of a hydrolysis circle of the strain on a tributyrin plate is 3, and the lipase activity reaches 136.81 U / mL. The invention also provides application of the strain and the lipase produced by the strain in preparation of tocopherol by catalyzing hydrolysis of tocopherol succinate. The strain STR7 is fermented to obtain crude enzyme liquid, the crude enzyme liquid is added into a reaction system containing tocopherol succinate for hydrolysis reaction, and after the reaction is performed for 9 hours, the content of tocopherol is increased by 865% compared with that of a control group. The strain and the method provided by the invention are high in catalytic efficiency, mild in reaction condition, environment-friendly and suitable for green industrial production of tocopherol.

This invention discloses a strain of *Pseudomonas fluorescens* STR2 and its applications, belonging to the field of microbial technology. The strain is classified as *Pseudomonas fluorescens* STR2, with accession number CCTCC NO: M 2025606. This strain exhibits a hydrolysis zone HC value of 4.23 on tributyrate plates and a lipase activity of 149.72 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR2 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

This invention discloses a method for preparing lanamivir I. The method includes: starting with N-acetylneuraminic acid, esterification to obtain II, followed by acetylation and cyclization without separation to obtain III; then deacetylation to obtain IV, followed by carbonate esterification to obtain V; etherification to obtain VI, followed by azide ring-opening to obtain VII; azide reduction to obtain VIII, followed by guanidinolation to obtain IX; then ester hydrolysis to obtain X; and finally deprotection of the Boc protecting group to obtain lanamivir I, with an overall yield of 25-46%. This invention uses thionyl chloride to catalyze methyl esterification, ensuring complete reaction without separation and allowing direct use in the next step; it utilizes trimethylsilyl trifluoromethanesulfonate as a catalyst to achieve a one-step acetylation and cyclization reaction, simplifying the operation; the azide ring-opening reaction conditions are mild, requiring no strict temperature control, and the yield can reach 88.9%; simultaneously, a crystallization purification step is introduced after guanidinolation, making the intermediate quality controllable, and the final product yield can reach over 85%.

This invention discloses a self-healing grout for building structures and its preparation process, belonging to the field of building materials technology. The grout comprises cement-based cementitious materials and a self-healing solvent powder. The self-healing solvent powder is prepared by grafting furfural-furan-methanol oligomers onto the surface of silanized wollastonite, and then compounding it with a lithium tetraborate-glycerol complex under acidic conditions. The dry grout comprises silicate cement, quartz sand, fly ash, silica fume, an expanding agent, the self-healing solvent powder, and a high-efficiency water-reducing agent. This invention utilizes an organic-inorganic hybrid supramolecular cross-linked microparticle structure, which sequentially undergoes borate ester hydrolysis, coordination dissociation, wollastonite dissolution, and calcium metaborate precipitation and expansion during crack seepage, achieving efficient crack filling and repeated self-healing. It is independent of microorganisms, exhibits strong robustness to cement-based highly alkaline environments, and the repair products form chemical bonds with the matrix, significantly improving the long-term durability and mechanical recovery capacity of the grout.

The application discloses a preparation method of fosnetupitant and intermediates thereof, which comprises the following steps: taking an amino thiophene compound II-a as a starting material, generating a chlorinated compound II-d through a plurality of reactions such as urea formation, ring closure and chlorination, then selectively catalytically hydrogenating and removing the chlorine at the C-4 position of the pyrimidine ring to obtain a compound II-e, and then preparing an intermediate compound II through ester hydrolysis and condensation with dimethylamine hydrochloride; taking a compound III-a and a compound III-b as starting materials to generate a substitution reaction to obtain a compound III-c, and then reducing the compound III-c through catalytic hydrogenation to obtain an intermediate compound III; and further generating a Buchwald-Hartwig coupling reaction of the intermediate compound II and the intermediate compound III to obtain a finished product of fosnetupitant. The preparation method can not only reduce the cost, simplify the synthesis route, control each reaction and improve the total yield, but also is more economic and environmental. Meanwhile, the application improves the stability and reliability of the process, and provides a guarantee for industrialized scale production of fosnetupitant.

Disclosed are an esterase for preparing sucralose and a use thereof. The amino acid sequence of the esterase is as shown in SEQ ID NO. 1, and the esterase can be used to catalyze the hydrolysis of sucralose-6-ethyl ester to prepare sucralose. The present invention further provides a gene encoding the esterase, a recombinant vector containing the gene, and a genetically engineered bacterium obtained by transforming the recombinant vector. The present invention is the first to apply the esterase having the amino acid sequence as shown in SEQ ID NO. 1 to the hydrolysis of sucralose-6-ethyl ester for preparing sucralose, and achieves excellent results, having an enzyme activity up to 5427 U / g, a substrate yield up to 100%, fewer by-products, a short reaction cycle, and a simple process, thereby greatly reducing production costs.

This invention discloses a strain of Bacillus wiedmannii STR18 and its applications, belonging to the field of microbial technology. The strain is classified as Bacillus wiedmannii STR18, with accession number CCTCC NO:M 2025611. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate plates and a lipase activity of 140 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR18 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 800% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

This invention discloses a method for preparing an all-weather biomimetic marine antifouling coating inspired by fluorescent squid, comprising the following steps: S1: preparation of UCN-Ag heterojunction powder; S2: synthesis of dynamic polyurethane prepolymer; S3: chain extension of dynamic polyurethane solution; S4: configuration and spraying of the composite coating. The coating of this invention achieves physical self-renewal of the surface based on ester hydrolysis, and autonomous repair of mechanical damage based on the reversible exchange of dynamic disulfide bonds and multiple hydrogen bonds. Simultaneously, it utilizes the synergistic effect of blue fluorescence excited by LAP in the dark with UCN-Ag to continuously generate reactive oxygen species under light-free conditions, further reducing bioadhesion. The antifouling coating prepared by this invention has a simple synthesis process and is environmentally friendly; it possesses advantages such as all-weather bactericidal and algal-resistant properties, autonomous damage repair, and long-term stability, and can be widely used for the protection of various marine equipment and facilities.

A process for extracting a compound selected from an alcohol or a carboxylic acid from an aqueous solution by forming an ester in the presence of at least one reagent selected from a carboxylic acid or an alcohol and an enzymatic catalyst, characterized in that the reaction mixture is in the form of a Pickering emulsion comprising at least a first water phase and an oil phase, said oil phase comprising at least one extraction solvent, said ester formed in the oil phase is then hydrolyzed to recover said compound to be extracted.

This invention discloses a strain of *Enterobacter cloacae* STR5 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* STR5, with accession number CCTCC NO: M 2025607. This strain exhibits a hydrolysis zone HC value of 3.95 on tributyrate plates and a lipase activity of 121.53 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR5 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The application belongs to the technical field of organic synthesis, and particularly relates to a synthesis process of 3-bromo-1-(3-chloro-2-pyridyl)-1H-pyrazole-5-carboxylic acid. The synthesis process takes maleic anhydride as a starting material, and 3-bromo-1-(3-chloro-2-pyridyl)-1H-pyrazole-5-carboxylic acid is obtained after esterification, bromination, acyl chlorination, cyclization, NBS bromination, DDQ oxidation, ester hydrolysis, acidification and refining, the atomic utilization rate is sufficient, and the product quality is excellent. In addition, the synthesis process has the advantages of strong stability, excellent bromination selectivity, mild and efficient oxidation reaction, industrialization friendliness, simple and controllable operation, low three-waste discharge, and is suitable for industrialized continuous production, and can be further popularized to the preparation of halogenated pyridyl pyrazole carboxylic acid compounds.