655 results about "Pseudomonas cruciviae" patented technology

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

The invention relates pseudomonas, use thereof and a method for removing cadmium pollution to the environment. The pseudomonas class is named pseudomonas putidaHN103 and the collection number in a collection center is CCTCCNo.M2011184; and the shape and characteristics of the pseudomonas putidaHN103 comprise: 1) on a culture medium, a bacterial colony which used to be colorless and transparent turns white, smooth and convex-like with irregular edges; and 2) according to the result of bacterial microscopic examination, pseudomonas belongs to brevibacterium and is gram negative. The use of the bacteria is the use of the bacteria as a biological repair material for removing cadmium pollution. The method for removing cadmium pollution to the environment comprises the following steps: 1) regulating the cadmium ion concentration in sewage to a range in which pseudomonas can grow normally; 2) placing pseudomonas; 3) keeping the pseudomonas in the sewage for a certain time period; and 4) removing bacterial absorbing cadmium ions. The pseudomonas requires small investment, is low in cost, high in adaptability and capable of effectively removing cadmium, can quickly and obviously lower the concentration of cadmium ions in the environment and is suitable for biological remediation environments polluted by cadmium.

The current invention provides high-affinity antibodies to the Pseudomonas aeruginosa PcrV protein that have reduced immunogenicity when administered to treat Pseudomonas aeruginosa infections.

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

The invention provides a plant bacteriostatic composition and application thereof in cosmetics. The plant bacteriostatic composition is obtained by compounding a licorice extract, a honeysuckle extract and a Mangnolia officinalis extract. The plant bacteriostatic composition has good bacteriostatic effect and particularly has a synergistic effect of inhibiting escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger. The plant bacteriostatic composition can be applied to skin-care and washing cosmetics, reduces the adding amount of original preservatives and enables a product to have both antibacterial and anti-inflammatory effects.

The invention discloses a sodium alginate compound immobilized microbial agent and a preparation method thereof, and the sodium alginate compound immobilized microbial agent solves the problem that an immobilization way of pseudomonas suitable for di-chlorine quinoline acid degradation in the prior art is absent. A carrier culture medium with the pseudomonas and sodium alginate are mixed to obtain a mixture, and the mixture is dropwise added into calcium chloride with concentration being 1-4% to stand for 2-4 hours to prepare a granule; the mass concentration of the sodium alginate is 2-6%; the carrier culture medium comprises an adsorption carrier and an inorganic salt liquid culture medium; a weight ratio of the adsorption carrier to the sodium alginate is (1-3) to 1; and the adsorption carrier consists of corn cob, bamboo charcoal and oil cake in a weight ratio being 1:(1-2):1. According to the invention, the degradation efficiency of bacteria is effectively improved, and the degradation rate of the immobilized microbial agent is much higher than the sum of the physical absorption efficiency of blank spheres and biological degradation efficiency of a free bacterial strain, so that the effect of mutual promotion can be achieved.

The invention relates to Pseudomonasputida WMQ-7, which has been collected in China Typical Model Cultivation Center (CCTCC) of Wuhan University on March 8, 2011 with the collection number of CCTCC M 2011060, and also relates to a method for separating and culturing the Pseudomonasputida WMQ-7 in hydrogen-absorbing enzyme-free soil of the rhizosphere of a leguminous plant. The method comprises the following steps of: enriching, separating and culturing the Pseudomonasputida WMQ-7 by using a culture device for continuously introducing H2 and a mineral salt culture medium, and screening strains with higher H2 oxidizing capacity by using gas chromatography. A hydrogen bacterium preparation is prepared from the optimal strains through fermented in a laboratory. The biological preparation can promote plant growth and improve yield, fertilizer efficiency and stress resistance of plants when used for agricultural production.

The invention provides mutated, cytotoxic forms of Pseudomonas exotoxin A (PE) comprising a furin cleavage sequence conjugated or fused directly to residues 395-613 of PE or variants of that sequence. These minimal forms of PE are smaller than previous cytotoxic forms of PE, reduce non-specific toxicity, and reduce immunogenicity due to domain II or domain Ib of PE. The invention further provides nucleic acids encoding said PEs, chimeric molecules containing them, and methods of use thereof.

The invention provides a foam dressing containing a composite silver-zinc antibacterial material and a preparation method of the foam dressing. The foam dressing containing the composite silver-zinc antibacterial material comprises polyurethane foaming plastic and the composite silver-zinc antibacterial material, wherein the composite silver-zinc antibacterial material is adhered to the inside of the polyurethane foaming plastic, comprises the following raw materials in content: nano silver, nano zinc and a nano inorganic carrier; and the composite silver-zinc antibacterial material contains the following raw materials in percentage by mass: 10%-20% of silver and 20%-40% of zinc. The composite silver-zinc antibacterial material is dispersed into the foam dressing in a manner of foam molding or ultrasonic dipping together with foam. The foam dressing containing the composite silver-zinc antibacterial material is capable of effectively absorbing diffusate of the wound surface, and effectively inhibiting growth and breeding of staphylococcus aureus, pseudomonas aeruginosa and candida albicans.

This invention relates to one kind of the antibacterial hydroxyl apatite compound coat, the preparation method and the application, its characteristic is the related compound coat is composed of the metal silver powder and the hydroxyl apatite powder, the metal silver powder is treated as the antibacterial increase ingredient, the silver powder' quality accounts for 1-5% of the compound coat, the particle size of the silver powder is 20-100mum, the particle size of the hydroxyl apatite powder is 10-100mum. This invention making the compound coat is realized by using the spray technics of the vacuum plasma. The provided compound coat has an above 95% excellent antibacterial effect on the coliform, the green pus pseudomonad and the golden yellow staphylococcus, and the cytotoxic rank in vitro of the compound coat is zero, has no cytotoxicity.

The invention discloses endophytic azotobacter of wheat producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application thereof. The endophytic azotobacter of wheat is pseudomonas sp. GDXM114, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.5040. The pseudomonas sp. GDXM114 with the collection number of CGMCC No.5040 has the advantages of higher nitrogenase activity, ACC deaminase activity and wide application prospect in producing nitrogenase microorganism inoculants and microbial organic fertilizers.

The present invention relates to one kind of genetic engineering bacteria, one kind of soybean disease causing variety of clove pseudomonads with preservation number of CGMCC 1276, and its construction process and use. The genetic engineering bacteria is obtained through inserting Tn5 transposon S17-1 into the genome of the initial strain to deactivate its temperature controlling gene. The strain may be used in industrial fermentation production of crown bacterin at 26-28 deg.c, in the yield near the initial bacteria and up to 35-40 mg/L.

the invention discloses a manufacturing method of rough rhamnolipid extract and application fermented by waste food and drink oil, which is characterized by the following: proceeding ultraviolet mutagenesis for natural sieved pseudomonas; picking fitful bacteria; adopting waste food and drink oil as ferment carbon source; making the density of rhamnolipid reach 35-45g/L; ultrafiltrating the ferment liquid to remove bacteria to obtain the powder-shaped rough extract through spraying and drying method; adding 0.01-3% rough extract in the biochemical dirty water to improve disposing efficiency.

The invention belongs to the technical field of microorganisms, and specifically relates to a strain of high-efficiency phosphate-resolving Pseudomonas and a microbial agent and application of the Pseudomonas. The strain was deposited on April 11, 2019 at General Microbiological Center of China Microbiological Culture Collection Management Committee, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposited number is CGMCC No. 17557. The bacteria can transform insoluble inorganic phosphorus into soluble available phosphorus with high efficiency, and the amountof dissolved phosphorus can reach 793 mg/L when calcium phosphate is used as the sole phosphorus source. The bacteria can be prepared into a phosphate-resolving microbial agent, which can be widely used in agricultural production, the content of the available phosphorus in soil can be effectively increased, the effect of saving fertilizer and increasing production can be achieved, and the microbial agent has good social, economic and environmental benefits.

A genetic recombinant method of pseudomonas aeruginosa for high-yield producing rhamnolipid includes following steps: (1) designing a primer for amplifying RhlAB gene according to the RhlAB gene sequence of rhamnolipid synthetase; (2) with chromosome of wild pseudomonas aeruginosa producing rhamnolipid as a template, performing PCR amplification to the RhlAB gene and performing rubber cutting purification to target genes through a purification kit; (3) performing enzyme digestion to the purified RhlAB gene to introduce the purified RhlAB gene into an escherichia coli-pseudomonas aeruginosa shuttle expression vector pAK1900 to obtain new plasmid pAK1900-AB; (4) introducing the constructed shuttle expression vector pAK1900-AB into a recipient bacterium pseudomonas aeruginosa competent cell through a heat-shock conversion method, coating a carboxyl-benzyl resistance plate with the competent cell and performing PCR verification to the constructed engineering bacteria; and (5) performing fermentation in a shake flask and measuring the yield of the rhamnolipid in a sulfuric acid-anthranone manner. The method can greatly reduce the production cost of the rhamnolipid, provides basis for industrial application of the rhamnolipid and can promote high-yield production of the rhamnolipid.

The invention relates to an exopolysaccharide generated by pseudomonas, a culture method and application. The pseudomonas sp. QL212 is preserved in common microbe center of China Committee for Culture Collection of Microorganisms on September 12, 2014, at the address of Institute of Microbiology of Chinese Academy of Sciences, No.3, Yard No. 1, West Beichen Road, Chaoyang District, Beijing, and having the preservation number of CGMCC NO.9651. The curdlan synthesized by the pseudomonas sp. QL212 serves as the exopolysaccharide; through fermentation condition optimization, the yield of curdlan is up to 5.94 g/L which is higher than the production peak of the already known pseudomonas curdlan.

The invention discloses a heterotrophic nitrification-aerobic denitrification pseudomonad strain, seed liquid obtained through culturing the strain, and a culture method. The strain is pseudomonad LJ9(pseudomonas indoloxydans LJ9), and is preserved in Guangdong culture collection center, the preservation time is 21 March 2018, and the preservation number is GDMCC NO:60339. The pseudomonad strainhas the capacity for resisting high carbon-nitrogen ratio and resisting high nitrate nitrogen and nitrite nitrogen, and has potential application value in the treatment problem of high-concentration organic waste water.

The invention relates to a culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and a fermentation method thereof, and a method for producing vitamin B12 by the same. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all of which contain artificial molasses composed of maltose, thiamine, riboflavin and biotin. By substituting the artificial molasses composed of maltose, thiamine, riboflavin and biotin for beet molasses and optimizing the formula of the culture medium, the invention solves the problem that the fluctuation range of fermentation titer is large because the quality of beet molasses is unstable, and acquires a method for stably and effectively producing vitamin B12. The invention saves the doses of raw and auxiliary materials, lowers the comprehensive cost and reduces the waste water discharge.