571 results about "Pseudomonas cruciviae" patented technology

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

The invention provides a recombinant vector for expressing LPL, a recombinant bacterium and an LPL production method, and belongs to the technical field of gene engineering. The invention provides a recombinant expression vector of Pseudomonas aeruginosa lipoprotein lipase LPL, wherein an LPL coding gene is derived from Pseudomonas aeruginosa. The recombinant expression vector is introduced into a pseudomonas aeruginosa PAO1 cell, an expression strain is successfully constructed, the expression quantity of target protein is improved, efficient expression of LPL in PAO1 is achieved, and the produced LPL is high in enzyme activity and wider in application range.

The invention discloses Pseudomonas sp. W50 and an application of the Pseudomonas sp. W50 in degradation of xanthate by combining the Pseudomonas sp. W50 with Cynanchum otophyllum. The Pseudomonas sp. W50 is preserved in Guangdong Microbial Culture Collection Center on July 31, 2024, and the preservation number of the Pseudomonas sp. W50 is GDMCC NO.64924. The Pseudomonas sp. W50 is preserved in the Guangdong Microbial Culture Collection Center on July 31, 2024. The degradation rate of 100mg / L BuX in 9h reaches up to 99%, and the strain also has various growth promoting characteristics of dissolving phosphorus, dissolving potassium, secreting IAA and GA and degrading cellulose. The BuX-polluted wastewater remediation agent can be used for remediation of BuX-polluted wastewater, can play a synergistic interaction role when being combined with pinkflower clover herb, has a more remarkable remediation effect, provides a new method for treatment of BuX pollution and promotion of healthy growth of aquatic plants in mining areas, and has a wide application prospect.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

Provided herein are systems for treating a subject with a pulmonary infection, for example, a nontuberculous mycobacterial pulmonary infection, a Burkholderia pulmonary infection, a pulmonary infection associated with bronchiectasis, or a Pseudomonas pulmonary infection. The system includes a pharmaceutical formulation comprising a liposomal aminoglycoside dispersion, and the lipid component of the liposomes consist essentially of electrically neutral lipids. The system also includes a nebulizer which generates an aerosol of the pharmaceutical formulation at a rate greater than about 0.53 gram per minute. The aerosol is delivered to the subject via inhalation for the treatment of the pulmonary infection.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention relates to application of ten antibacterial peptides in the antimicrobial field. The ten antibacterial peptides have strong antibacterial activity on multi-drug-resistant bacteria, including but not limited to gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, klebsiella pneumoniae and the like, and gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis and the like. Activity research proves that any one of the ten antibacterial peptides has a remarkable effect of inhibiting, killing or preventing microbial infection. Due to the strong antibacterial property of any antibacterial peptide, the antibacterial peptide has wide application potential in the aspects of preparing antibacterial drugs, preservatives, antibacterial coatings, antibacterial fibers, antibacterial packaging materials and the like. Due to the unique property of the antibacterial peptide in the aspect of antibacterial performance, a new direction is provided for the development of efficient and reliable antibacterial agents and antibacterial technologies and products in multiple fields.

The invention relates to the technical field of microbial degradation, and discloses application of pseudomonas aeruginosa in degradation of polypropylene plastic and a method for degrading the polypropylene plastic. The pseudomonas aeruginosa is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO: 27456. The pseudomonas aeruginosa has relatively high degradation efficiency and relatively high degradation rate on polypropylene plastics, and has a good market application prospect.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention belongs to the field of food processing, and particularly relates to an ostrich meat preservative and a preservation method. The ostrich meat preservative provided by the invention is prepared by matching and mixing the chitosan, the nisin and the tea polyphenol according to a specific proportion, so that the refrigeration refreshing time of the ostrich meat treated by the preservative can be effectively prolonged, the ostrich meat can maintain a relatively high L * value and a * value within 16 days of storage, the deterioration of meat color is delayed, the water-retaining property is remarkably improved, and the quality of the ostrich meat is improved. The content of thiobarbituric acid reactants and volatile basic nitrogen is obviously reduced, and fat oxidation and protein degradation are inhibited; the total number of bacterial colonies and the number of microorganisms such as coliform bacteria, pseudomonas, mould and saccharomycetes are obviously reduced. The growth and reproduction of putrefying bacteria can be effectively inhibited, and the cold fresh shelf life is prolonged.

The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.

The invention relates to the field of microorganisms, and in particular relates to pseudomonas sp. JKM2 for producing ACC (Acetobacter Cellulose) deaminase as well as application and a use method of the pseudomonas sp. JKM2. The strain is preserved in the China General Microbiological Culture Collection Center on October 15, 2024, and the preservation number is CGMCC NO: 32219. The pseudomonas sp. JKM2 capable of producing the ACC deaminase, disclosed by the invention, can be used for relieving soil compaction stress and promoting plant growth. According to the ACC deaminase-producing pseudomonas sp. As well as the application and the use method thereof, an ethylene synthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC) can be degraded, so that the ethylene content of a plant root system under soil compaction stress is effectively reduced, plant growth is promoted, the content or synthesis of IAA and ABA of the root system under soil compaction stress can be increased, the morphology of the plant root system is adjusted, and the growth of the plant is promoted. The tolerance of the plants to the soil compaction stress is enhanced.

The invention discloses a preparation method and application of a cow milk antibacterial peptide with wide inhibitory activity on gram-positive bacteria and gram-negative bacteria by a fermentation method, the cow milk antibacterial peptide is prepared by taking milk protein as a raw material through a microbial fermentation method and a multi-stage membrane separation and purification process, the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%, and the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%. And the peptide content in the product is 60-85%. The cow milk antibacterial peptide prepared by the preparation method disclosed by the invention has inhibitory activity on gram-positive bacteria and gram-negative bacteria. The prepared cow milk antibacterial peptide can be used for preparing drugs and / or health care products, food and skin care products for preventing and / or reducing infectious diseases caused by escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, porphyromonas gingivalis and streptococcus mutans, can also be used as a food preservative, an oral bacteriostatic agent, a preservative and the like, and has a good application prospect.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention discloses Pseudomonas protegens for producing nitropyrrole, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC (China Center for Type Culture Collection) M20241968. The invention further discloses a preparation method of the Pseudomonas protegens for producing nitropyrrole. The invention also discloses an engineering bacterium for producing nitropyrrole and a construction method of the engineering bacterium. The engineering bacterium is a mutant strain for blocking a bypass metabolic pathway of the pseudomonas. For example, the pseudomonas is taken as a target, a core gene in a BGC1 biosynthetic gene cluster in the pseudomonas is knocked out through a gene knockout method, or a core gene in a BGC16 biosynthetic gene cluster is knocked out, or a mutant strain of the core genes in two biosynthetic gene clusters of BGC1 and BGC16 is knocked out at the same time. The invention also discloses a biocontrol microbial inoculum containing the pseudomonas or the engineering bacteria. The invention further discloses application of the pseudomonas or the engineering bacteria or the biocontrol microbial agent in prevention and treatment of rhizoctonia solani, and the pseudomonas or the engineering bacteria or the biocontrol microbial agent has a remarkable antibacterial effect.

The invention discloses a strain of disease-resistant and growth-promoting pseudomonas avenae LC40 and application thereof, and relates to the technical field of microorganisms, the pseudomonas avenae is specifically pseudomonas avenae LC40, the pseudomonas avenae LC40 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32869. The invention further discloses a method for preparing the disease-resistant and growth-promoting pseudomonas avenae LC40. The bacterial strain can be used for inhibiting tobacco mosaic virus (TMV), tomato brown fruit ruffle virus (ToBRFV), rice blast germ (Magnaporthe oryzae) and alternaria tenuissima (Alternaria tenuissima), and the bacterial strain can be used for inhibiting the tobacco mosaic virus (TMV), the tomato brown fruit ruffle virus (ToBRFV), the rice blast germ (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae) and the allium tenuissima (Alternaria tenuissima). The Pseudomonas Asian LC40 provided by the invention has phosphorus solubilizing capacity and can secrete auxin compounds IAA, and field test results show that the LC40 strain has the effects of promoting growth and increasing yield of tomatoes. The pseudomonas Asian LC40 strain can effectively prevent and control plant diseases and promote crop growth, so that the yield and quality of crops are improved, and the pseudomonas Asian LC40 strain shows good application prospects of disease prevention and growth promotion.

The invention belongs to the technical field of microorganisms, discloses a strain of pseudomonas putida and application of the strain of pseudomonas putida as fish probiotics, and particularly discloses a strain of pseudomonas putida. The invention provides a symbiotic pseudomonas putida CD separated from fish, and the strain has relatively strong fat utilization capability, can generate a plurality of beneficial extracellular metabolites, can promote metabolism of fish lipids, carbohydrates and proteins and weight gain, and also can improve the iron content and hemoglobin content in fish tissues. The pseudomonas putida strain CD disclosed by the invention has good biological safety, and has a wide development prospect in the aspect of application as fish probiotics.

The invention discloses an application of NSC348884 in preparation of an antibacterial drug, the bacteria of the NSC348884 are gram-negative bacteria or drug-resistant gram-negative bacteria, and the gram-negative bacteria are acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa or klebsiella pneumoniae; based on an antibacterial drug high-throughput screening technology, it is found from 1280 small molecule compounds that the benzimidazole compound NSC348884 has direct bacteriostasis and sterilization effects on gram-negative bacteria and drug-resistant gram-negative bacteria, and no drug resistance is generated after the benzimidazole compound NSC348884 is continuously applied for 14 days. Further research shows that NSC348884 can interfere with lipid synthesis of gram-negative bacterium cell membranes, inhibit formation of biological membranes and induce lipid metabolism disorder, so that bacteriostatic and bactericidal effects are achieved. In addition, the NSC348884 can also enhance the sensitivity of the drug-resistant bacteria to the imipenem and the polymyxin B.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention discloses Pseudomonas putida, a bacterial agent thereof and an application of the Pseudomonas putida in chemical wastewater treatment, the Pseudomonas putida is named as PH-20 and preserved in the China General Microbiological Culture Collection Center on March 17, 2025, and the preservation number of the strain is CGMCC No. 33831. The invention further discloses a preparation method of the Pseudomonas putida. The pseudomonas putida disclosed by the invention can be used for rapidly degrading polybenzoic acid such as terephthalic acid, phthalic acid, isophthalic acid, trimellitic acid, trimellitic acid and pyromellitic acid in wastewater under the conditions of high salt and low temperature, and the degradation effect is still excellent under the condition that various pollutants coexist.

The invention discloses broad bean pseudomonas, a fungicide and application of the broad bean pseudomonas in biological control of diseases and plant growth promotion, and relates to the technical field of biocontrol bacteria. The preservation number of the broad bean pseudomonas is CGMCC (China General Microbiological Culture Collection Center) No.32657. The strain has growth promoting and biocontrol functions, can effectively prevent and treat various Chinese herbal medicine and crop diseases and promote plant growth, and has a good application prospect.

The invention belongs to the technical field of biology, and particularly relates to application of hepcidin expression quantity in larimichthys crocea liver as larimichthys crocea visceral white-spot disease resistance evaluation index and a construction method. The hepcidin expression quantity in the larimichthys crocea liver is applied as the larimichthys crocea visceral white-spot disease resistance evaluation index. The construction method of the resistance evaluation index comprises the following steps: taking healthy small yellow croaker intraperitoneal injection pseudomonas proteinus strain bacterial liquid, and determining a half lethal dose in 96 hours; the method comprises the following steps: injecting a half lethal dose of bacterial liquid of pseudomonas proteinus strains into the abdominal cavity of healthy small yellow croaker for 96 hours, and after infection, taking different tissues to carry out hepcidin gene expression fluorescent quantitative PCR analysis; a regression model of gene expression quantity and bacterium loading quantity is established through fluorescence quantification, and the relationship between hepcidin expression quantity and disease resistance is constructed. Accurate disease-resistant phenotype information is provided for development of breeding of visceral white-spot disease-resistant improved varieties of the small yellow croakers, the accuracy of disease-resistant breeding is promoted, early detection of diseases is realized, and prevention and treatment of the diseases are effectively guided.

The invention relates to the field of microorganisms, in particular to application of a pseudomonas strain y2 in synthesis of polyhydroxyalkanoate, a microbial agent prepared from the pseudomonas strain y2 is added into wastewater to be treated, and the pseudomonas strain y2 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC M 20231228. The strain realizes microbial efficient synthesis of PHA, has good synthesis capability and environmental adaptability when being used for PHA synthesis, and can realize efficient synthesis of PHA in a pure culture state. The chemical wastewater contains abundant organic matters and can be used as a carbon source for synthesizing the PHA by the pseudomonas strain y2, so that the production cost of the PHA is further reduced, a higher PHA synthesis yield is obtained, and a feasible way is provided for reducing the industrial production cost of the PHA.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.