64 results about "Pseudomonas cruciviae" patented technology

This invention discloses a composite microbial agent with both efficient ferulic acid degradation and growth-promoting functions, and its preparation method. The active component of this composite microbial agent contains *Pseudomonas soybeanii* with accession number CGMCC No. 36468. Pseudomonas soyae Strain A9, and *Pseudomonas aeruginosa* with accession number CGMCC No. 36469. Paraburkholderia oxyphila Strain A23. This invention, by screening and combining two functionally complementary strains, fundamentally solves the technical problems of existing single-strain ferulic acid degrading bacteria agents having limited function and unstable field application efficacy, achieving improved degradation efficiency and synergistic growth promotion effects.

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The application discloses a pseudomonas strain TR14 and application thereof in degrading nicotine, and the pseudomonas strain TR14 is obtained by being separated from soil in which tobacco is planted perennially, has good degrading capacity on nicotine, and has a degrading rate of 98.44% on 7g / L nicotine after being cultured for 24 hours, and the highest tolerance reaches 8g / L nicotine. The strain can effectively degrade nicotine content in upper tobacco leaves.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

The application discloses a Pseudomonas atacamensis STR8 and an application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas atacamensis STR8, and the preservation number is CCTCC NO: M 2025609. The hydrolysis circle HC value of the strain on a tributyrin plate is 2.78, and the lipase activity reaches 139.86 U / mL. The application also provides an application of the strain and a lipase produced by the strain in catalyzing hydrolysis of tocopherol succinate to prepare tocopherol. The strain STR8 is fermented to obtain a crude enzyme liquid, and the crude enzyme liquid is added into a reaction system containing the tocopherol succinate to perform a hydrolysis reaction. After 9 hours of reaction, the content of the tocopherol is increased by 750% compared with a control group. The strain and the method provided by the application have high catalytic efficiency, mild reaction conditions and environmental friendliness, and are suitable for green industrialized production of the tocopherol.

This invention belongs to the field of bioengineering and provides a method for constructing *Pseudomonas aeruginosa* strains that produce 2-hydroxyphenazine and its applications. This invention uses Qlu-1-3 from patent ZL202011026637.X as the starting strain, and achieves this by knocking out a gene that inhibits phenazine synthesis in the genome. mdtA , gspM The gene enabled the engineered strain to produce 2-hydroxyphenazine at concentrations of 156.3 mg / L and 216.4 mg / L, laying the foundation for the subsequent production of 2-OH-PHZ using Pseudomonas aeruginosa.

A genetically engineered strain of Pseudomonas is provided, which is capable of metabolizing lactose as a sole carbon source in the production of polyhydroxyalkanoates (PHAs). A method of engineering microorganisms and a method of PHA production are also provided, wherein lactose is used as a sole carbon source.

The application discloses a flagellum inhibiting peptide of pseudomonas aeruginosa and a preparation method and application thereof, and the sequence of the flagellum inhibiting peptide is Lys-Ile-Gly-Leu-Phe-Arg-Trp-Arg. The synthetic inhibiting peptide has a time-dependent self-assembly ability, can gradually evolve from scattered granular into filamentous structure, and finally forms a stable net-like morphology. The synthetic inhibiting peptide can significantly inhibit the flagellum motility of PAO1 and other strains of pseudomonas aeruginosa, and has synergistic antibacterial activity when combined with allicin, ciprofloxacin, meropenem, levofloxacin, polymyxin B and the like. Moreover, the synthetic inhibiting peptide has obvious bacteriostatic and healing-promoting effects on burn wound infection.

The application discloses an N-(3-cyclobutyrolactone)-4-nitrophenyl butyryl amide and amikacin composition and application of the composition in treating chronic infection of drug-resistant bacteria. After the N-(3-cyclobutyrolactone)-4-nitrophenyl butyryl amide and the amikacin are combined, not only the sensitivity of pseudomonas aeruginosa to the amikacin can be obviously increased, but also the formation amount of the pseudomonas aeruginosa biofilm can be greatly reduced, so that the composition can be applied to preventing and treating chronic infection of drug-resistant pseudomonas aeruginosa.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

Provided are the screening and identification of a Pseudomonas sp. strain for producing feruloyl esterase at high yield and a use of the Pseudomonas sp. strain as a bioaugmentation agent in fermented foods, relating to the technical field of brewing. By means of screening, a Pseudomonas sp. strain A-4H for producing feruloyl esterase at high yield is obtained. After cultivation for 36 h, the Pseudomonas sp. strain A-4H can produce ferulic acid at a concentration of 153.79±2.36 mg / L, and the activity of feruloyl esterase in a fermentation broth reaches 136.63±10.27 U / L. The addition of the Pseudomonas sp. A-4H during the brewing of Huangjiu can effectively increase the content of ferulic acid, the content of phenolic acid, and the content of organic acid in Huangjiu, providing an effective biological pathway for increasing the content of ferulic acid in fermented foods.

The application discloses a mandelonitrile-degrading bacterium SYY40 and a culture method and application thereof, relates to the technical field of microbial pollution degradation and environmental pollution, and provides the mandelonitrile-degrading bacterium SYY40 which is identified as Pseudomonas sp. SYY40 and named Pseudomonas sp. SYY40, and is preserved in the China General Microbiological Culture Collection Center on May 19, 2025, and the preservation number of the strain is CGMCC NO. 34596. The strain can realize efficient removal of mandelonitrile, and the degradation rate of the mandelonitrile reaches 100% within 48 hours.

A compound microbial agent for enhancing corn yield, its preparation method, and its application are disclosed, relating to the fields of agriculture and biotechnology. Replacing traditional chemical fertilizers with this compound microbial agent can effectively improve crop growth and reduce environmental pollution. The compound microbial agent comprises a compound bacterial solution and a compound carrier. The compound bacterial solution includes a suspension of Enterobacter and a suspension of Pseudomonas, while the compound carrier includes wood ash, vermiculite, sodium carboxymethyl cellulose, trehalose, and humic acid. The method involves mixing the Enterobacter and Pseudomonas suspensions to form the compound bacterial solution; then mixing the compound bacterial solution with the compound carrier at a 1:1 volume ratio, air-drying, maturing, and adjusting the moisture content to obtain the compound microbial agent. This agent can improve soil physicochemical properties, promote corn growth, and achieve the goal of increasing yield and income.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

The application provides a pseudomonas koreensis WF3 and a saline-alkali soil microbial fertilizer, a preparation method and application thereof, and belongs to the technical field of biological composting.The preparation method of the saline-alkali soil microbial fertilizer provided by the application comprises the following steps: (1) configuring a fertilizer carrier comprising matured organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; (2) inoculating the pseudomonas koreensis WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the saline-alkali soil microbial fertilizer.The pseudomonas koreensis WF3 has the abilities of producing extracellular polysaccharides and organic acids, and can survive in a strong saline-alkali environment; the fertilizer carrier is fermented by the pseudomonas koreensis WF3; the prepared microbial organic fertilizer can effectively improve the soil organic carbon, total nitrogen and water-stable large aggregate (>2.5mm) content, increase the corn yield, reduce the soil pH and total salt content, and has a remarkable improvement effect on the saline-alkali soil.

This invention discloses a salt-tolerant, alkali-tolerant, and hydrocarbon-loving composite functional microbial agent for the remediation of oil-containing saline-alkali land, its preparation method, and its application. It relates to the field of environmental microbiology and soil bioremediation technology. The composite microbial agent is composed of halophilic Bacillus, alkaliphilic Pseudomonas, and petroleum hydrocarbon-degrading Mycobacterium, compounded in a viable count ratio of 3:2:2, with a single strain having a viable count ≥ 5.0 × 10⁻⁶. 9 This invention, with a concentration of CFU / g, can tolerate extreme environments including 12% NaCl concentration and pH 9.5. Through targeted screening and precise formulation of functional bacterial strains, it achieves a synergistic effect of salt and alkali tolerance and broad-spectrum petroleum hydrocarbon degradation. It can be widely applied in oilfield ecological restoration projects, recultivation of saline-alkali polluted farmland, and in-situ bioremediation of oil- and salt-alkali co-contaminated soils. The preparation process is simple, and the bacterial cell activity retention rate is high, solving the technical problems of poor stress resistance and low degradation efficiency of traditional degrading agents. It possesses excellent remediation effects and industrial application value.

This invention belongs to the field of biotechnology, specifically relating to a *Pseudomonas putida* genome insertion site library and its construction method. This invention, for the first time, systematically screened and obtained 17 specific genome insertion sites suitable for *Pseudomonas putida*. The constructed insertion site library covers different expression intensities, filling the gap in existing technologies lacking efficient integration sites for *Pseudomonas putida*, and providing diverse options for personalized expression of target genes. The screened insertion sites are all located in the regions of opposing transcription genes, avoiding interference with the host's basal metabolic network. The recombinant strain exhibits stable growth performance without significant growth inhibition, making it suitable for large-scale industrial applications. The integration method, based on homologous recombination, achieves stable integration of the target gene without plasmid maintenance, avoiding expression instability caused by plasmid loss and copy number fluctuations. It also reduces the continuous use of antibiotics, lowering industrialization costs and environmental risks, and the construction method is simple and efficient.

The present application relates to a kind of detection methods of strong putrefaction Pseudomonas lundensis, comprising extracting nucleic acid in the sample to be measured;Then with nucleic acid as template, using specific RAA primer pair is recombined enzyme-mediated amplification, and obtains amplification product;RAA primer pair targets the aprX gene of Pseudomonas lundensis comprising single nucleotide polymorphism site associated with strong putrefaction phenotype;Finally, the amplification product is added to CRISPR / Cas detection system, incubated at constant temperature, then real-time detects its fluorescence signal;Detection system includes Cas protein, specific crRNA and fluorescent reporter probe, and crRNA can recognize single nucleotide polymorphism site associated with strong putrefaction phenotype in Pseudomonas lundensis aprX gene.The present application improves the detection accuracy, and detection process is simple to operate, and reaction system is simple, not only improves detection efficiency, also reduces detection cost.

This invention relates to the field of genetic engineering technology, and provides a method for high-yield production of 2-hydroxyphenazine using Pseudomonas aeruginosa, using strain Qlu-1ΔHΔPΔO as the starting strain, and knocking out pykA Genes were used to obtain strain Qlu-1ΔHΔPΔO-1; pykA The gene sequence is shown in SEQ ID NO. 1. This invention uses *Pseudomonas aeruginosa* Qlu-1ΔHΔPΔO from ZL 202210461035.X as the starting strain, and employs a scarless knockout method... pykA Genes were used to obtain the engineered strain Qlu-1ΔHΔPΔO-1. After fermentation and yield testing, it was found that the 2-hydroxyphenazine yield of Qlu-1ΔHΔPΔO-1 was increased to 321.2 mg / L, thus achieving an increase in the yield of 2-hydroxyphenazine in the strain.

The application discloses application of BMS-833923 and derivatives thereof and a medicine, and belongs to the technical field of antibiotics. The BMS-833923 and the derivatives thereof can be used as colistin adjuvants to jointly inhibit or eliminate gram-negative bacteria, and can also independently inhibit or eliminate gram-positive bacteria. The gram-negative bacteria can include at least one of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, pan-drug-resistant strains BAA-1800, BAA-1794 and BAA-1792, and the gram-positive bacteria can include at least one of Staphylococcus aureus and Bacillus subtilis. By taking the BMS-833923 and the derivatives thereof as adjuvants of colistin, the therapeutic index of the colistin can be effectively expanded, and lower, non-toxic doses of the colistin can be used in clinical treatment of effective treatment of drug-resistant bacterial infections.

This invention relates to an antimicrobial peptide and its applications. The amino acid sequence of the antimicrobial peptide is at least one of the sequences shown in SEQ ID No. 1 to SEQ ID No. 8. The antimicrobial peptide exhibits excellent broad-spectrum antimicrobial activity, for example, against Acinetobacter spp. (…). Acinetobacter ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) and Bacillus spp. ( Bacillus This bacterium possesses antibacterial activity. Against the backdrop of increasingly severe global bacterial resistance and the gradual ineffectiveness of traditional antibiotics against multidrug-resistant bacteria, this provides a new treatment option for carbapenem-resistant bacteria such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Bacillus subtilis, which pose a significant threat to public health, and offers new possibilities for solving the clinical dilemma of "no available drugs."

This invention proposes a method for the biosynergistic extraction and refined utilization of all active ingredients in ginseng, belonging to the fields of medical, dental, or cosmetic formulations. This invention utilizes ginseng stress extract obtained through specific microbial treatment. This method leverages the stress stimulation of *Pseudomonas fluorescens* to efficiently synthesize various saponins, polysaccharides, and volatile oils in ginseng, resulting in an extract rich in components and with enhanced activity. Combining fractional extraction, resin purification, and nanoemulsification processes, a skincare raw material with comprehensive components, high stability, and excellent bioavailability is ultimately obtained, exhibiting a powerful synergistic effect in anti-inflammatory and anti-aging properties.

This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

This invention provides a SNP molecular marker related to Pseudomonas recruitment in cucumber and its application, belonging to the field of microbial technology. The SNP molecular marker is closely related to the enrichment of Pseudomonas in cucumber. Haplotype cucumbers with the SNP molecular marker CCA recruit Pseudomonas more efficiently than haplotype cucumbers with the SNP molecular marker TTG, increasing the abundance of Pseudomonas in the xylem. This invention further demonstrates that by applying a *Pseudomonas chrysogenum* inoculant to different haplotype cucumbers, the *Pseudomonas chrysogenum* inoculant significantly increases the number of *Pseudomonas chrysogenum* in haplotype cucumbers with the SNP molecular marker CCA, more significantly promoting cucumber growth and increasing plant height, stem diameter, leaf area, fresh weight, dry weight, and the content of 4-methylene glutamine.

This invention discloses a strain of *Alcaligenes* HHR-1-1 and its application in the degradation of carotenoids and the preparation of flavorings, relating to the field of microbial fermentation technology. The *Alcaligenes* strain of this invention (… Pseudomonas alcaligenes The HHR-1-1 strain has the accession number CCTCC NO: M 20252745, and the *Alcaligenes* HHR-1-1 strain was deposited at the China Center for Type Culture Collection on December 1, 2025. The *Alcaligenes* HHR-1-1 strain can degrade carotenoids with a degradation rate of 80%–97%, and its main degradation products are β-ionone and its oxides, and dihydroactinolone. Furthermore, the fermentation broth of *Alcaligenes* HHR-1-1 strain and carotenoids can be used in flavor preparation, effectively improving the quality of the flavors.

This invention belongs to the field of genetic engineering technology, specifically relating to an engineered strain of *Pseudomonas* that regulates rhizosphere colonization and phosphorus solubilization, its construction method, and its applications. This invention achieves gene-specific expression through a phosphorus starvation-inducible promoter and knocks out… crp The gene reduces metabolic burden, increases colonization rate and phosphorus solubility, and its functional activity does not significantly decrease under extreme environments. The engineered strain constructed in this invention has growth-promoting effects on grain crops such as wheat and corn, as well as specialty economic crops such as strawberries and tomatoes. It is tolerant to extreme environments such as severe salinity, low temperature, strong acidity, low oxygen, and low nutrition. It can activate various insoluble phosphorus compounds such as tricalcium phosphate, phytate phosphorus, and ferric phosphate, breaking through the application limitations of existing strains in single crops, soils, and phosphorus forms.

This invention provides a method for treating farmland drainage using an aerated ecological filter, relating to the field of wastewater treatment technology. The aerated ecological filter comprises, from top to bottom, a coarse-particle packing layer, an aeration device, a filter screen, a Fe-BC@CS-MOF porous material layer, another filter screen, and a fine-particle packing layer. Pseudomonas taiwanensis and Pseudomonas chengduensis are introduced into the aerated ecological filter for cultivation and acclimatization, which converts nitrate nitrogen in farmland drainage into ammonia nitrogen and degrades organic pollutants, reducing COD in the water. The Fe-BC@CS-MOF porous material layer can adsorb ammonia nitrogen, phosphate, and heavy metals. Ammonia nitrogen and phosphate are desorbed through acid leaching, while heavy metals are retained in the porous material, allowing for the recovery and reuse of ammonia nitrogen and phosphate.