336 results about "Pseudomonas cruciviae" patented technology

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention discloses an application of NSC348884 in preparation of an antibacterial drug, the bacteria of the NSC348884 are gram-negative bacteria or drug-resistant gram-negative bacteria, and the gram-negative bacteria are acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa or klebsiella pneumoniae; based on an antibacterial drug high-throughput screening technology, it is found from 1280 small molecule compounds that the benzimidazole compound NSC348884 has direct bacteriostasis and sterilization effects on gram-negative bacteria and drug-resistant gram-negative bacteria, and no drug resistance is generated after the benzimidazole compound NSC348884 is continuously applied for 14 days. Further research shows that NSC348884 can interfere with lipid synthesis of gram-negative bacterium cell membranes, inhibit formation of biological membranes and induce lipid metabolism disorder, so that bacteriostatic and bactericidal effects are achieved. In addition, the NSC348884 can also enhance the sensitivity of the drug-resistant bacteria to the imipenem and the polymyxin B.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention belongs to the technical field of biology, and particularly relates to application of hepcidin expression quantity in larimichthys crocea liver as larimichthys crocea visceral white-spot disease resistance evaluation index and a construction method. The hepcidin expression quantity in the larimichthys crocea liver is applied as the larimichthys crocea visceral white-spot disease resistance evaluation index. The construction method of the resistance evaluation index comprises the following steps: taking healthy small yellow croaker intraperitoneal injection pseudomonas proteinus strain bacterial liquid, and determining a half lethal dose in 96 hours; the method comprises the following steps: injecting a half lethal dose of bacterial liquid of pseudomonas proteinus strains into the abdominal cavity of healthy small yellow croaker for 96 hours, and after infection, taking different tissues to carry out hepcidin gene expression fluorescent quantitative PCR analysis; a regression model of gene expression quantity and bacterium loading quantity is established through fluorescence quantification, and the relationship between hepcidin expression quantity and disease resistance is constructed. Accurate disease-resistant phenotype information is provided for development of breeding of visceral white-spot disease-resistant improved varieties of the small yellow croakers, the accuracy of disease-resistant breeding is promoted, early detection of diseases is realized, and prevention and treatment of the diseases are effectively guided.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention relates to the field of biodiesel catalytic materials, and particularly discloses a catalyst for kitchen waste oil, a preparation method and biodiesel. The catalyst for the kitchen waste oil is prepared from a Fe3O4-SiO2-Pr < 3 + > nano-particle carrier and a lipase solution, the catalyst is prepared from a Pr (NO3) 3 solution doped with Pr < 3 + > being 0.88-0.92 wt%, the lipase solution is a pseudomonas lipase solution with the concentration being 22-28 mg / mL, and the pH is 7.0; the preparation method comprises the following steps: preparing Fe3O4 nanospheres, coating the surfaces of the Fe3O4 nanospheres with SiO2 layers, dipping the Fe3O4 nanospheres in a Pr (NO3) 3 solution, carrying out calcination treatment to obtain a Fe3O4-SiO2-Pr < 3 + > nanoparticle carrier, placing the carrier in a lipase solution, carrying out adsorption fixation, and separating out the carrier to obtain the catalyst. The catalyst is used for processing biodiesel and has the advantages of being easy to recycle, high in loading capacity, low in activity loss and good in stability.

The invention discloses bacillus safensis and application thereof to synergistically promoting soybean root nodule formation and yield increase with pseudomonas, the bacillus safensis is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34634, the preservation date is May 22, 2025, the preservation number is CGMCC No.34634, the preservation number is CGMCC No.34634, the preservation number is CGMCC No.34634, and the preservation number is CGMCC No.34634. The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. According to the method disclosed by the invention, the increase of nodules and the increase of nitrogenase activity can be promoted by co-inoculating the bacillus safensis and the pseudomonas, and meanwhile, the increase of the yield of a biological membrane is also promoted by co-inoculating.

The invention discloses pseudomonas capable of dissolving phosphorus and producing siderophores and application of the pseudomonas, and relates to the technical field of microorganisms. The bacterial strain P-14-21 is identified as a new species of Pseudomonas sp. And is named as Pseudomonas sp. P-14-21, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024, and the preservation number is GDMCC No: 64969. The invention also discloses a preparation method of the Pseudomonas sp. P-14-21, and the bacterial strain is named as Pseudomonas sp. P-14-21. The bacterial strain is named as Pseudomonas sp. P-14-21 and is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024. Besides, the pseudomonas P-14-21 disclosed by the invention has the capacity of dissolving organic phosphorus and high-yield siderophore, the phosphorus dissolving index can reach 2.4, and the activity unit (su) of the siderophore can reach 94.41%, so that the strain has excellent development and application values in agricultural production.

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

The present invention relates to the pharmaceutical field, and provides a boric acid β-lactamase inhibitor or a use thereof. The boric acid β-lactamase inhibitor is a compound shown in formula (I), or an optical isomer or a pharmaceutically acceptable salt thereof: formula (I). The ultra-broad-spectrum boric acid β-lactamase inhibitor of the present invention has an inhibition effect on A, B, C, and D enzymes, and carbapenem drug-resistant bacteria such as Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa.

The invention discloses an aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83 and application thereof, the strain is classified and named as Pseudomonas protegens, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.36443. The invention further discloses a preparation method of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The invention further discloses an application of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The strain has outstanding ammonia nitrogen metabolism capability under an aerobic condition, can quickly and efficiently convert ammonia nitrogen in a water body into a harmless or low-toxicity form, has wide tolerance and adaptability to environmental factors such as pH, temperature and the like, and is stable in performance. The strain is suitable for bioremediation of natural water, domestic sewage and various industrial wastewater, can effectively improve the denitrification efficiency and the system stability, and has the advantages of simplicity and convenience in operation, environmental friendliness and high practical application value.

The present invention discloses an application and method of a SPILR protein for improving plant resistance to Pseudomonas syringae, belonging to the field of agricultural science and technology. Specifically, the method comprises using Pseudomonas syringae to induce expression of the SPILR protein in Arabidopsis leaves, constructing an expression vector pCambia1300-SPILR containing the nucleotide sequence of the SPILR protein, transforming the expression vector into Agrobacterium tumefaciens GV3101 competent cells, culturing the cells, and infecting plant leaves to obtain Pseudomonas syringae-resistant plants. Therefore, genetic engineering techniques can be used to cultivate resistant varieties.

The application discloses a method for inhibiting growth of pseudomonas by using fucoidan and application thereof, and is characterized in that the fucoidan is a degradation product of natural seaweed (sodium alginate), and is a linear oligosaccharide with a polymerization degree of 2-25 and composed of guluronic acid and mannuronic acid, and has the advantages of safety, health and no toxic side effects; in combination with the research of the inventors that the fucoidan can target and inhibit growth of pseudomonas in vitro and in intestinal flora, the fucoidan can meet the demand of practical application, can be used for delaying corruption and deterioration of fresh food caused by pseudomonas, and can be used for inhibiting growth of pseudomonas on the surface or in the body of aquatic products or fresh food, and has good application prospect and safety.

The present application relates to the field of oil production, discloses a microbial wax removal and prevention agent, and a preparation method and application thereof, the microbial wax removal and prevention agent comprises a nutrient solution and microorganisms; the microorganisms comprise Exiguobacterium aurantiacum, and at least one of Bacillus stearothermophilus, Pseudomonas schneideri, Bacillus licheniformis and Rhodococcus ruber.The microbial wax removal and prevention agent is suitable for both ordinary wax-containing oil well wax removal and prevention and high calcium and magnesium wax-containing oil reservoir, has efficient wax dissolving and prevention effect, and has a wide application range.

The present invention relates to a polyhydroxyalkanoate synthase (PHA synthase) mutant derived from Pseudomonas 6-19 (KCTC 11027BP) which can produce a lactate ester polymer and / or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for producing a lactate ester polymer and / or copolymer using the said synthase mutant. The polyhydroxyalkanoate synthase mutant of the present invention derived from Pseudomonas 6-19 can effectively produce a lactate ester polymer and / or copolymer by using lactyl-CoA as a substrate which is difficult to use as a substrate with a conventional polyhydroxyalkanoate synthase.

This invention discloses a composite microbial agent with both efficient ferulic acid degradation and growth-promoting functions, and its preparation method. The active component of this composite microbial agent contains *Pseudomonas soybeanii* with accession number CGMCC No. 36468. Pseudomonas soyae Strain A9, and *Pseudomonas aeruginosa* with accession number CGMCC No. 36469. Paraburkholderia oxyphila Strain A23. This invention, by screening and combining two functionally complementary strains, fundamentally solves the technical problems of existing single-strain ferulic acid degrading bacteria agents having limited function and unstable field application efficacy, achieving improved degradation efficiency and synergistic growth promotion effects.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The invention discloses a bacteriophage capable of efficiently splitting Pseudomonas ongdamura and a composition and application thereof, the bacteriophage is Pseudomonas ongdamura bacteriophage PL1P1, the preservation number is CCTCCM20251191, the Pseudomonas ongdamura bacteriophage PL1P1 is a virulent bacteriophage separated from the nature, and the Pseudomonas ongdamura bacteriophage PL1P1 has relatively high tolerance to ultraviolet rays and pH, and can be used for efficiently splitting Pseudomonas ongdamura and Pseudomonas ongdamura and Pseudomonas ongdamura. The bacteriophage is suitable for different prevention and treatment environments and can achieve a good fresh-keeping effect on chilled meat, DNA of the bacteriophage cannot encode protein possibly causing potential health risks, the possibility of carrying lysogenic genes does not exist, the bacteriophage has high affinity and splitting capacity, the titer of 10 < 10 > PFU / mL or above can be achieved within 24 h of culture, and the bacteriophage has good application prospects. The Pseudomonas longdamura phage PL1P1 can specifically partially or completely inactivate Pseudomonas longdamura, can complete mass proliferation only by using a small amount of initial phage, and provides a high-quality phage strain source for industrial production of phage bactericides.

The invention discloses an application of a DMP3 gene and an encoding protein thereof in improving plant disease resistance, and belongs to the technical field of gene engineering. The DMP3 gene and the encoding protein thereof are used for improving the disease resistance of plants, the nucleotide sequence of the DMP3 gene is shown as SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the DMP3 gene is shown as SEQ ID NO: 2, especially the disease resistance of the plants to pseudomonas syringae. The invention further provides a PAD3 gene promoter induced by pathogenic bacteria and a plant expression vector containing the PAD3 gene promoter induced by the pathogenic bacteria and a DMP3 gene, wherein the sequence of the PAD3 gene promoter is shown as SEQ ID NO: 3. Real-time quantitative PCR (polymerase chain reaction) shows that Pseudomonas syringae infection remarkably induces up-regulation of expression of a DMP3 gene of a plant, functional experiments show that DMP3 gene expression can activate immune response of the plant, and overexpression of DMP3 causes death of the plant.

The application discloses a pseudomonas strain TR14 and application thereof in degrading nicotine, and the pseudomonas strain TR14 is obtained by being separated from soil in which tobacco is planted perennially, has good degrading capacity on nicotine, and has a degrading rate of 98.44% on 7g / L nicotine after being cultured for 24 hours, and the highest tolerance reaches 8g / L nicotine. The strain can effectively degrade nicotine content in upper tobacco leaves.

The invention belongs to the technical field of solid waste recycling, and discloses efficient carbon sequestration pseudomonas stutzeri from an oil-containing solid waste residue source and application of the efficient carbon sequestration pseudomonas stutzeri, and the efficient carbon sequestration pseudomonas stutzeri is pseudomonas stutzeri A58-5 with the preservation number of CCTCC NO: M 20241582. The Pseudomonas stutzeri is obtained by taking Pseudomonas stutzeri A58 separated from oil-containing solid waste residues of an oil field as an original strain and carrying out ARTP mutagenesis and screening, has the excellent characteristics of reducing pollution and sequestering carbon and is relatively strong in stress resistance; the method has wide application prospects in the fields of deep recycling (soil conversion) of oil-containing solid waste residues, improvement of the carbon sequestration capacity of the petroleum-contaminated soil, remediation of the petroleum-contaminated soil and the like, and is of great significance in pollution reduction and sequestration increase in the petroleum and petrochemical industry under the background of supporting double carbon.

The invention belongs to the technical field of biology, and provides a pseudomonas aeruginosa endotoxin specific binding polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID No. 1, SEQ ID No. 3 or SEQ ID No. 4 in a sequence table. Experiments prove that the polypeptide has excellent affinity with pseudomonas aeruginosa endotoxin, and compared with an existing anti-endotoxin clinical drug polymyxin B, the polypeptide has obviously better pseudomonas aeruginosa endotoxin detoxification capability. On the basis, the invention further provides application of the polypeptide in removal or separation or analysis of the endotoxin of the pseudomonas aeruginosa and application of the polypeptide in preparation of a detoxification medicine for the endotoxin of the pseudomonas aeruginosa. The polypeptide can be widely applied to the biomedical fields of biological separation, biological detection, disease diagnosis and treatment and the like.