The invention belongs to the technical field of microorganisms, and specifically relates to a strain of high-efficiency phosphate-resolving Pseudomonas and a microbial agent and application of the Pseudomonas. The strain was deposited on April 11, 2019 at General Microbiological Center of China Microbiological Culture Collection Management Committee, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposited number is CGMCC No. 17557. The bacteria can transform insoluble inorganic phosphorus into soluble available phosphorus with high efficiency, and the amountof dissolved phosphorus can reach 793 mg/L when calcium phosphate is used as the sole phosphorus source. The bacteria can be prepared into a phosphate-resolving microbial agent, which can be widely used in agricultural production, the content of the available phosphorus in soil can be effectively increased, the effect of saving fertilizer and increasing production can be achieved, and the microbial agent has good social, economic and environmental benefits.