Composite bacterial preparation as well as preparation method and application thereof

A technology of compound bacterial agents and strains, which is applied in the field of agricultural biology, can solve the problems of nitrogen reducing compost quality, human and animal health hazards, and cannot be used alone, so as to achieve increased nitrogen accumulation, strong ammonia suppression ability, and reduce ammonia production. Effect

Inactive Publication Date: 2011-09-28
SICHUAN AGRI UNIV
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  • Summary
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Problems solved by technology

[0006]Generally, composting poultry and livestock manure with high fiber content can greatly shorten the composting cycle by using compound bacterial agents represented by EM bacterial agents, but due to the It is a kind of poultry manure with high protein content. The rapid and massive decomposition of nitrogen-containing organic substances during the decomposing process will cause a large amo...

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  • Composite bacterial preparation as well as preparation method and application thereof
  • Composite bacterial preparation as well as preparation method and application thereof
  • Composite bacterial preparation as well as preparation method and application thereof

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preparation example Construction

[0040] Three, the preparation of composite bacterial agent:

[0041] The present invention enumerates the preparation method of composite bacterial agent as follows:

[0042] (1) Slant culture: Inoculate the isolated strains into agar medium under sterile conditions, use beef extract medium for Bacillus brevis and Pseudomonas fluorescens, and improve Gaoshi No. 1 for Thermoactinomyces sugarcane Culture medium, cultivated in an incubator at 45°C to 50°C for 1 to 4 days;

[0043] (2) First-level seed cultivation: the strains obtained in step (1) were inoculated under aseptic conditions in liquid medium corresponding to various bacteria (beef extract medium was used for bacteria, and improved Gao's No. 1 medium), under the condition of 45°C-50°C (optimum temperature is 50°C), 100-200r / min shaker culture for 1-4 days, to obtain first-class seeds;

[0044] (3) Secondary seed cultivation: Inoculate primary seeds in fermenters respectively, sterilize at 120-125°C (the optimum tempe...

Embodiment 1

[0052] The compound microbial agent that this embodiment enumerates is used to process pig manure, and it is made up of the following components of volume ratio: 15 parts of Bacillus brevis fermentation bacteria liquid, 40 parts of Pseudomonas fluorescens fermentation bacteria liquid, sugarcane thermal radiation 45 parts of bacterium fermented bacterium liquid, prepare according to the preparation method of above-mentioned composite bacterium agent. There are about 400 million bacteria or actinomycetes in every mL of fermentation broth.

[0053] After the compound bacterial agent is cultivated, add pig manure compost to ferment according to the specific method of using the above-mentioned composite bacterial agent for pig manure composting. Samples were taken and analyzed on the 2nd, 5th, 10th, 15th, and 20th days of composting and fermentation, and the boric acid absorption solution was replaced every 2 to 3 days. A certain amount of deionized water was added to the fresh co...

Embodiment 2

[0055] The compound microbial agent that this embodiment enumerates for processing pig manure is made up of the following components of volume ratio: 40 parts of Bacillus brevis fermentation bacteria liquid, 15 parts of Pseudomonas fluorescens fermentation bacteria liquid, sugarcane thermal radiation 45 parts of bacterial fermentation liquid. There are about 400 million bacteria or actinomycetes in each mL of fermentation broth.

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Abstract

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

Description

technical field [0001] The invention relates to a composite bacterial agent and its preparation method and application, in particular to a composite bacterial agent for treating pig manure, its preparation method and application, and belongs to the field of agricultural biotechnology. Background technique [0002] With the continuous large-scale development of the breeding industry, a large amount of poultry manure produced seriously pollutes the environment and restricts economic development, which has become an important issue of ecological and environmental protection that cannot be ignored. In 2000, the output of livestock and poultry manure in my country reached 3.64 billion tons, which was 3.8 times the amount of industrial solid waste in the same period. According to statistics, the daily excretion of feces and urine of a pig is 6kg, which is 5 times that of human excrement and urine, and the annual output of feces and urine is about 2.5t. With the improvement of peo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/08C12R1/39C12R1/01
CPCY02W30/40
Inventor 王昌全李冰曾晰菀杨娟曾敏周锐李启权袁大刚
Owner SICHUAN AGRI UNIV
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