Composite bacterial preparation as well as preparation method and application thereof
A technology of compound bacterial agents and strains, which is applied in the field of agricultural biology, can solve the problems of nitrogen reducing compost quality, human and animal health hazards, and cannot be used alone, so as to achieve increased nitrogen accumulation, strong ammonia suppression ability, and reduce ammonia production. Effect
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[0040] Three, the preparation of composite bacterial agent:
[0041] The present invention enumerates the preparation method of composite bacterial agent as follows:
[0042] (1) Slant culture: Inoculate the isolated strains into agar medium under sterile conditions, use beef extract medium for Bacillus brevis and Pseudomonas fluorescens, and improve Gaoshi No. 1 for Thermoactinomyces sugarcane Culture medium, cultivated in an incubator at 45°C to 50°C for 1 to 4 days;
[0043] (2) First-level seed cultivation: the strains obtained in step (1) were inoculated under aseptic conditions in liquid medium corresponding to various bacteria (beef extract medium was used for bacteria, and improved Gao's No. 1 medium), under the condition of 45°C-50°C (optimum temperature is 50°C), 100-200r / min shaker culture for 1-4 days, to obtain first-class seeds;
[0044] (3) Secondary seed cultivation: Inoculate primary seeds in fermenters respectively, sterilize at 120-125°C (the optimum tempe...
Embodiment 1
[0052] The compound microbial agent that this embodiment enumerates is used to process pig manure, and it is made up of the following components of volume ratio: 15 parts of Bacillus brevis fermentation bacteria liquid, 40 parts of Pseudomonas fluorescens fermentation bacteria liquid, sugarcane thermal radiation 45 parts of bacterium fermented bacterium liquid, prepare according to the preparation method of above-mentioned composite bacterium agent. There are about 400 million bacteria or actinomycetes in every mL of fermentation broth.
[0053] After the compound bacterial agent is cultivated, add pig manure compost to ferment according to the specific method of using the above-mentioned composite bacterial agent for pig manure composting. Samples were taken and analyzed on the 2nd, 5th, 10th, 15th, and 20th days of composting and fermentation, and the boric acid absorption solution was replaced every 2 to 3 days. A certain amount of deionized water was added to the fresh co...
Embodiment 2
[0055] The compound microbial agent that this embodiment enumerates for processing pig manure is made up of the following components of volume ratio: 40 parts of Bacillus brevis fermentation bacteria liquid, 15 parts of Pseudomonas fluorescens fermentation bacteria liquid, sugarcane thermal radiation 45 parts of bacterial fermentation liquid. There are about 400 million bacteria or actinomycetes in each mL of fermentation broth.
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