200 results about "Brevibacillus" patented technology

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention discloses a compound microbial fertilizer and application thereof in field planting. The survival rate of the paenibacillus mucilaginosus HX-02 disclosed by the invention can be up to 62.8% after 170d under the condition of 38% of nitrogen, phosphorus and potassium. The strain is organically combined with bacillus velezensis RKN1111 and a nitrogen-phosphorus-potassium inorganic fertilizer (N + P2O5 + K2O = 38%), the compound microbial fertilizer is developed, the viable count of effective bacteria is not less than 1.5 billion / g, and the viable count of paenibacillus mucilaginosus is not less than 0.5 billion / g. The compound microbial fertilizer taking the strain as a main functional bacterium is applied to field corn planting, can replace part of chemical fertilizers and increase soil fertility, can reduce the use amount of the chemical fertilizers by 15.56%, and achieves the effects of promoting the growth of field crops and increasing the yield by 10.2%. The paenibacillus mucilaginosus HX-02 is separated from soil, is harmless to human, livestock and environment, and meets the requirements of green plant protection and agricultural sustainable development.

The invention discloses bacillus velezensis Scm3 and application thereof.The bacillus velezensis Scm3 is obtained by screening a strain Scm with an inhibition effect on aeromonas hydrophila from turbot intestinal contents and then conducting ultraviolet mutagenesis on the strain to obtain a strain Scm3 with an enhanced antagonism effect on aeromonas hydrophila, and the strain Scm3 is applied to the preparation of the bacillus velezensis Scm3 and the application of the bacillus velezensis Scm3 to prepare the bacillus velezensis Scm3. 16SrDNA (deoxyribonucleic acid) sequence identification and genome sequencing prove that the strain is bacillus velezensis, and the inhibition mechanism of the bacillus velezensis Scm3 on aeromonas hydrophila is researched by a non-contact co-culture method.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention provides brevibacillus laterosporus LY15-1 and application of the brevibacillus laterosporus LY15-1, the brevibacillus laterosporus LY15-1 is preserved in China General Microbiological Culture Collection Center on February 8, 2025, and the preservation number of the brevibacillus laterosporus LY15-1 is CGMCC No. 33504. The brevibacillus laterosporus disclosed by the invention has excellent effects of promoting growth and preventing and treating root rot at the same time.

The invention relates to the technical field of plant tissue culture seedling raising, in particular to paenibacillus feed and application thereof in promoting cunninghamia lanceolata test-tube plantlet height growth. The feed paenibacillus paenibacillus SY0317 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 20575, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation time is August 31, 2020. The cunninghamia lanceolata test-tube plantlet cultured by adopting the strain has high growth speed, the growth amount is increased by more than 60%, the culture time of the cunninghamia lanceolata tissue culture plantlet is shortened, large-scale production of the cunninghamia lanceolata tissue culture plantlet can be realized, and the strain has remarkable economic and social benefits.

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention relates to brevibacillus parabrevis and application thereof in tissue isolation, and belongs to the technical field of biology. The brevibacillus parabrevis JN-6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.29951, and the preservation date is March 7, 2024. The brevibacillus parabrevis JN-6 disclosed by the invention has the advantages that the preservation number is CGMCC NO.29951; the brevibacillus parabrevis provided by the invention has collagen degradation capability, and the produced collagenase can be used for adipose tissue separation and is further cultured into adipose-derived stem cells. When the produced collagenase is used for digestion and separation, the number of obtained cells can be 6.17 * 10 < 6 > / mL, the adipose-derived stem cells can be effectively differentiated, and the method has a good application prospect.

The invention discloses a complex microbial inoculant for promoting lignocellulose degradation and a preparation method and application thereof, and relates to the technical field of microorganisms, the complex microbial inoculant comprises brevundimonas sp. AFBI (Brevundimonas sp.), alcaligenes faecalis AFBII (Alcaligenes faecalis), Achromobacter AFBIII (Achromobacter sp.), Staphylococcus aureus GBSI (Staphylococcus sp.), Glutamic Bacter GBS II (Glutamic Bacter sp.) and Bacillus (Bacillus sp.). According to the composite microbial agent provided by the invention, all strains have no antagonistic action and are mutually cooperated, and lignocellulose can be efficiently and stably degraded.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention relates to the technical field of bio-fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula as well as a preparation method and application of the bio-organic fertilizer. The bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula provided by the invention comprises a kitchen fermentation base material and a complex microbial inoculant in a weight ratio of 100: (1-10), the complex microbial inoculant comprises one or more of bacillus atrophaeus, bacillus coagulans, bacillus licheniformis, bacillus velezensis and brevibacillus laterosporus; the invention further provides a preparation method of the bio-organic fertilizer and application of the bio-organic fertilizer in prevention and treatment of root rot of codonopsis pilosula. The bio-organic fertilizer provided by the invention can degrade glyphosate in soil on one hand, and also can reduce the occurrence of root rot of codonopsis pilosula on the other hand, so that the growth of codonopsis pilosula and the accumulation of active value components of codonopsis pilosula can be promoted.

The invention discloses paenibacillus alvei NNUB1, a fungicide and application of the paenibacillus alvei NNUB1. The paenibacillus alvei NNUB1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28005. The invention further discloses a preparation method of the paenibacillus alvei NNUB1. The newly separated paenibacillus alvei NNUB1 has excellent activity of resisting soil transmission plant pathogens, not only can antagonize various fusarium fungi, but also can effectively inhibit lauraceae lauraceae; the strain also has the characteristic of promoting plant growth, and the biomass of plants can be obviously improved; test data show that the strain can effectively inhibit soil-borne pathogens of continuously-cultivated soil, remarkably promote crop growth and has an excellent application prospect.

The invention relates to a method for in-situ remediation or management and control of a petroleum contaminated site by micro-nano aeration enhanced microorganisms, which comprises the following steps: 1) injecting micro-nano bubbles into soil or underground water, and continuously aerating for 3-5 days for 6-10 hours every day; an air source of the micro-nano bubbles is one or a combination of two of air and oxygen; 2) 12-24 hours after the step 1) is completed, a compound microbial agent is injected into the soil or the underground water, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, leucas, phenyl bacilli and brevibacillus in the viable count ratio of (1-25): (1-15): (1-5): 1. According to the method, the energy consumption is low, secondary pollution is avoided, the remediation or control effect is excellent, and the total petroleum hydrocarbon (C10-C40) pollutant concentration of the contaminated site after remediation is remarkably reduced.

The present invention discloses a composite microbial agent capable of improving the nitrogen fixation ability of plants and its application, belonging to the field of agricultural production. The composite microbial agent is composed of Brevibacillus sp. GD296 and Bacillus velezensis DPT-03, and the ratio of the effective viable bacteria count of the two is 1.2:1. Also disclosed is a composite microbial fertilizer, which comprises the following components in parts by weight: 30-35 parts of soybean meal powder, 45-50 parts of rice husk powder, 8-10 parts of corn flour, 1-3 parts of alkaline soil conditioner, and the above-mentioned composite microbial agent, and the total effective viable bacteria count of the composite microbial agent in the composite microbial fertilizer is ≥ 350 million / g. Through experiments, it is found that the composite microbial agent is a combination of nitrogen-fixing bacteria and non-nitrogen-fixing bacteria, and the two have a promoting effect and can improve the nitrogen fixation effect; and using the composite microbial fertilizer prepared with the above-mentioned composite microbial agent can also significantly improve the growth of plants in alkaline soil.

The invention provides a microbial agent for preventing and treating nematode diseases as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the microbial agent is mainly prepared from the following raw materials in percentage by mass: 60-75% of microbial thalli; 25%-40% of an additive; wherein the microbial thalli are prepared from purpureocillium lilacinum, bacillus subtilis, brevibacillus laterosporus, bacillus mucilaginosus and lactobacillus sporogenes. Through the collaborative design of a specific strain combination and a functional additive, the stress resistance and colonization ability of the microbial inoculum in a complex soil environment can be effectively improved, meanwhile, an efficient synergistic mechanism of fungi and bacteria is established, the microbial inoculum can adapt to complex soil types, and the control effect on nematodes can be effectively improved. The invention fundamentally solves the industrial obstacles of poor environmental adaptability, short lasting period, single function and the like of biological agents, and provides a solution for large-scale application for chemical pesticide substitution.

The invention discloses bacillus velezensis, a microbial preparation as well as a preparation method and application of the microbial preparation, and relates to the technical field of microorganisms. The bacillus velezensis strain is preserved in the China Center for Type Culture Collection, and is named as bacillus velezensis XNW-BLSYB1, the preservation number is CCTCC NO: M 2025721, the preservation time is April 8, 2025, and the 16S rDNA sequence of the bacillus velezensis strain is as shown in SEQID No.1. The bacillus velezensis strain has the advantages that the bacillus velezensis strain is named as bacillus velezensis XNW-BLSYB1; the spore formed by the bacillus velezensis XNW-BLSYB1 strain can survive at high temperature, and can inhibit the reproductive and respiratory syndrome virus and inhibit the activity of bacteria such as streptococcus suis, infectious actinobacillus pleuropneumoniae, clostridium perfringens, pasteurella multocida and escherichia coli.

The invention relates to the technical field of gene engineering, in particular to a D-psicose-3-epimerase mutant and application of the D-psicose-3-epimerase mutant in synthesis of psicose. According to the present invention, the 59th site, the 106th site and the 188th site of the wild type D-psicose-3-epimerase derived from Brevibacillus thermophilus (Brevibacillus thermophilus) are subjected to simultaneous mutation to obtain the D-psicose-3-epimerase mutant, such that the efficiency of producing D-psicose by using fructose as the raw material is significantly improved, the reaction of catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the yield of the D-psicose is significantly improved; the conversion rate is up to 36.5%; according to the present invention, the coexpression binding enzyme strain is constructed by coupling glucose isomerase, such that the intermediate transfer efficiency and the enzyme stability are improved, the reaction for producing D-psicose through catalysis of 500 g / L glucose is performed for 6 h, and the conversion rate can achieve 30.5%; and the activity of the immobilized enzyme can still be kept at 80% after the immobilized enzyme is repeatedly used for 10 batches.

The invention relates to an antibacterial peptide LK-KJT-B01 for gram-negative bacterium infection and a preparation method and application thereof.The antibacterial peptide LK-KJT-B01 can be extracted from bacillus laterosporus, has the advantages of being broad in spectrum, safe, free of toxicity and the like, has a very strong bactericidal effect on gram-negative bacterium infection, and can be used for preparing antibacterial peptide LK-KJT-B01 for gram-negative bacterium infection. Particularly, the sensitivity to carbapenem-resistant gram-negative bacteria is high, and the application prospect is very wide.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The present invention discloses a method for reducing and fixing Cr(VI) by key functional metal-reducing microorganisms based on a mutualistic symbiotic model: S1, preparing a mixed functional bacterial community electrolyte consisting of conductive bacteria and metal-reducing functional bacterial communities, wherein the conductive bacteria is Shewanella, and the metal-reducing functional bacterial communities include one or more of Bacillus cereus, Ralstonia pietermaritzii, and Paenibacillus alvei; S2, enriching an electroactive biofilm: pouring the prepared mixed functional bacterial community electrolyte into an electrolytic cell in a three-electrode bioelectrochemical system, and applying a positive potential of 100 to 300 mV; S3, reducing and fixing Cr(VI): after generating a mature anode biofilm with a stable current output, pouring out the electrolyte in the electrolytic cell, adding a new electrolyte containing Cr(VI), and applying a negative potential of 500 to 0 mV to the working electrode, so that the Cr(VI) in the electrolyte is reduced and fixed.

The invention relates to the technical field of kitchen waste treatment, and particularly discloses compound bacteria and application thereof in kitchen waste recycling. The compound bacteria disclosed by the invention comprise a component A and a component B, the component A is formed by mixing bacillus velezensis, bacillus amyloliquefaciens, pseudomonas, bacillus atrophaeus, brevibacillus laterosporus and trichoderma harzianum; and the component B is prepared by mixing bacillus parlicheniformis, bacillus licheniformis, urea bacillus thermophilus and fat bacillus thermophilus. According to the technical scheme of resourceful treatment on the kitchen garbage by using the compound bacteria provided by the invention, the degradation effect of the kitchen garbage can be effectively improved, and the purposes of reduction, harmlessness and resourceful treatment of the kitchen garbage and energy consumption reduction in the treatment process are achieved; and the kitchen waste resourceful treatment material has an excellent application effect as a soil conditioner and a feed.

The invention provides a bacillus strain which is named as bacillus sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37528, and the preservation date is January 26, 2026. The bacillus strain is named as bacillus sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC). The 16S rRNA (ribosomal Ribonucleic Acid) nucleotide sequence of the bacillus sp. Is as shown in SEQ ID NO: 1. Applications are also provided for promoting plant growth and improving plant quality. The bacillus sp has the capabilities of dissolving phosphorus, secreting IAA (Indoleacetic Acid) and producing siderophores, can promote plant growth and improve plant quality, and is used for promoting the growth of new wheat straw seedlings.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention relates to the field of microorganisms and application, in particular to brevibacillus mat, a microbial agent containing the brevibacillus mat and application of the brevibacillus mat. The invention provides a brevibacillus sschleri strain KY987 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the brevibacillus sschleri strain KY987 is CGMCC No. 37625. The strain not only has the activity of killing main pathogenic bacteria of soybean root rot, but also has broad-spectrum bactericidal activity on various plant pathogenic bacteria. In addition, the strain also has caenorhabditis elegans killing activity, is easy to grow, and is suitable for realizing large-scale industrial application.

The application relates to a soil Brevibacillus solid-state fungicide as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating seed liquid of soil Brevibacillus M7 into a fermentation medium to culture, so as to obtain a fermentation liquid; centrifuging the fermentation liquid to obtain bacterial slurry; uniformly mixing the bacterial slurry with a protective agent solution to obtain a suspension, and then drying to obtain the soil Brevibacillus solid-state fungicide; in the preparation process, specific fermentation culture and specific composite protective agents are adopted, so that the activity of the bacterial body in the solid-state fungicide can be greatly reserved, high-activity solid-state fungicide is obtained, and the storage time is long. The solid-state fungicide still maintains high activity after being stored for 12 months.

The invention provides a method for improving pollination rate and podding rate of alfalfa, and belongs to the technical field of biology. The invention provides a strain of salt-tolerant Bacillus brevis EB3, the preservation number of which is CGMCC No.33617, and further provides a microbial inoculum composition composed of the strain and marine yeast, a composition composed of the microbial inoculum composition and a pollen nutrient solution, and a method for improving the pollination rate and the pod bearing rate of alfalfa by using the composition. The method effectively solves the problems of low setting rate of alfalfa, abortion of florets, serious flower drop and the like, can significantly improve the number of alfalfa pollen and the germination rate of the pollen, and reduces the drop rate of the florets, so that the effect of significantly improving the seed yield of the alfalfa is achieved, and the method is simple to operate, stable in effect and low in cost.

This disclosure relates to compositions and methods for controlling personal care odors. More specifically, this disclosure relates to compositions comprising a ferment extract of Brevibacillus laterosporus or a fraction thereof for controlling body odor and methods for using the same. Compositions containing a ferment extract of Brevibacillus laterosporus are suitable for use as underarm / axillary deodorants and for odor control in personal care applications.