319 results about "Brevibacillus" patented technology

Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

The invention discloses a clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system-based bacillus licheniformis genome editing vector. A preparation method of the bacillus licheniformis genome editing vector comprises the steps of integrating a Cas9 protein expression cassette and a small guide ribonucleic acid (sgRNA) scaffold expression cassette into a starting vector pHY xyl to obtain pHY-Cas9-sgRNA scaffold; then, integrating any one of multiple sgRNA fragments, which are designed and synthesized by taking amyL as a target site, into the pHY-Cas9-sgRNA scaffold to obtain the bacillus licheniformis genome editing vector. The CRISPR-Cas9 system-based bacillus licheniformis genome editing vector can greatly improve the current situation that at present, the gene editing of bacillus licheniformis is difficult, and obviously increases the gene editing efficiency.

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

Bacteria of the sp. Bacillus that produce a lipopeptide are found to be effective in the treatment and prophylaxis of gastro-intestinal disease when administered as a probiotic. In particular, a strain of Bacillus bacteria identified as PB6 is useful for the treatment of Antibiotic Associated Diarrhea (AAD) or the more serious condition Clostridium difficile associated diarrhea (CDAD) when administered as a probiotic. Additionally, these bacteria have been found efficient for the treatment of immunorelated diseases such as Inflammatory Bowel Disease (IBD).

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The invention discloses a Brevibacillus borstelensis Po CGMCC No. of 2441 and the application thereof. A microbial inoculum that contains the brevibacillus borstelensis Po CGMCC No. of 2441 also belongs to the extent of protection of the invention. Model oil displacing experiment results indicate that after the oil displacement with polymers, the brevibacillus borstelensis oil displacement can be used for raising the recovery factor by 3 to 5 percent (OOIP), the brevibacillus borstelensis oil displacement combined with a polymer protecting slug can be used for raising the recovery factor by about 7 percent (OOIP), and a brevibacillus borstelensis oil displacing method that is combined with chemical oil displacement can be used for raising the recovery factor by 6 to 13 percent (OOIP). The experiment results indicate that the strain can be widely applied into the field of oil exploiting industries and particularly into the field of enhanced microorganism oil exploiting, and is applicable to large-area popularization and application.

The invention provides a kind of Brevibacillus laterosporus (STP-B.latCGMCCNo.1119), the character follows this. G+, haulm shape, the shape is (0.4-0.5)*(2-2.5)mu. The spore adnation is from neutrality to sub-neutrality. The spore bursa will enlarge. Most of the spore is vertebra aitch, the remainder is disciform. VP-, ph value of culture fluid is 7.2, amylolysis-, gelatin dispergation-, decomposition casein+, katalase+, oxidase-, cirate -, dextrose aerogenesis-, dextrose acidgenesis+, L-arabinose acidgenesis-, xylose acidgenesis-, mannit acidgenesis+, azotate deocidisation+, methyl red+, pH5.7 growth+.

The invention discloses a compost inoculant and applications thereof. The active ingredients of the composting inoculant provided by the invention include clostridium thermocellum, geobacillus stearothermophilus, Taiwan false xanthomonas, brevibacillus agri and bacillus licheniformis in a CFU (colony-forming unit) ratio of (3-5):(1-2):(1-2):(1-2):(1-2). Strains in the inoculant provided by the invention can grow in a symbiosis state well, and under the conditions of microaerobic co-culturing, lignocelluloses can be regularly proliferated and effectively decomposed in proportion. The inoculant is simple in manufacturing process due to co-culturing, stable in decomposition function, and long in quality ensured period. Experiments show that when the inoculant is adopted for fermenting composts, large gas feeding is not required, the energy is saved, and the nitrogen loss is reduced; composts can be thoroughly decomposed in advance, thereby improving the quality of fertilizers.

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

The present invention belongs to the technical field of environment protection, in particular to a method to degrade Microcystic aeruginosa through microorganisms. The method is that firstly an algae-lysing bacterial strain Brevibacillus spp.FDK2 can be achieved by separating from and sublimating deep eutrophication urban lake water body;the algae-lysing bacterial strain Brevibacillus spp.FDK2 is cultured by a culture medium to acquire bacterial liquid with a certain concentration. The bacterial liquid is added into a water sample containing Microcystic aeruginosa. Under a certain condition, the bacterial strain has a good degrading effect on the Microcystic aeruginosa. The degrading removing rate is more than 90 percent.

The invention discloses a compound microbial agent with a function of effectively degrading cellulose at high temperature. The compound microbial agent contains five actinomycetes: Streptomyces thermoviolaceus, Streptomyces thermodiastaticus, Streptomyces thermocarboxydus, Streptomyces albidoflavus and Streptomyces thermovulgaris, and one bacteria: Brevibacillus borstelensis. The compound microbial agent solves the problem of slow composting and long cycle of garden waste due to the limitation in cellulose degradation difficulty, accelerates cellulose degradation in the composting process, shortens the fermentation period, and improves the conversion efficiency, thereby having a broad application prospect in the aspects such as the application of composting treatment and resource utilization of garden waste, and the like.

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function on pathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content of organic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobacco bacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×1010 cfu / g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.6% of biocontrol rate of tobacco bacterial wilt in the soil suffering from tobacco bacterial wilt. If the microbial organic fertilizer is applied in the soil without continuous cropping obstacle in a long time, it can prevent the occurrence of the bacterial wilt of continuous cropping tobacco to a great extent.

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

This invention provides compositions of Bacillus strains and methods for using such compositions to inhibit the activity and / or growth of fungal pathogens of plants. In one embodiment, this invention provides a composition comprising Bacillus bacteria selected from the group consisting of Brevibacillus laterosporus strain CM-3, Brevibacillus laterosporus strain CM-33, Bacillus amyloliquefaciens BCM-CM5, Bacillus licheniformis ATCC-11946, Bacillus mojavensis BCM-01, Bacillus pumilus NRRL-1875, Bacillus subtilis 10 DSM-10, Bacillus subtilis NRRL-1650, Bacillus megaterium BCM-07, Paenibacillus polymyxa DSM-36, Paenibacillus chitinolyticus DSM-11030, and combinations thereof. In another embodiment, this invention provides a method for preparing a bacterial composition comprising one or more Bacillus strains by growing Bacillus strain bacteria until the bacteria form spores, collecting said spores, and formulating said composition.

The invention discloses a salt-tolerant Brevibacillus brevis and an application of complex microorganisms thereof in advanced treatment of high salt content degradation-resistant oil extraction sewage. The salt-tolerant Brevibacillus brevis is reserved in China General Microbiological Culture Collection Center, CGMCC for short, and the preservation number is CGMCC No.2148. The bacillus can bear high salinity which reaches 70000ppm, and has a wide applicable temperature range. The Brevibacillus brevis has a high COD degradation rate for petroleum hydrocarbon and salt-containing and oil-containing degradation-resistant sewage, and at 25-45 DEG C, the COD degradation rate for high salt content sewage (whose mineralization is higher than 30000ppm) reaches 60%. Brevibacillus brevis, Bacillus subtilis (registration number KC109003.1), Bacillus circulans Jordan (registration number JX893033.1), Bacillus amyloliquefaciens (registration number JX997921.1), Pseudomonas stutzeri (registration number KC113178.1) and Pseudomonas mendocina Marinobacter (registration number EU43329.1) compose complex microorganisms. A synergism is provided for high efficiency degradation of high salt content and oil containing degradation-resistant oil extraction sewage; the COD of incoming water is lower than 350ppm, and the COD of the discharged water after treatment is lower than 60ppm.

The invention provides a bacterial strain LT3 producing alkaline cellulose and a breeding method and an initial optimization of cellulose production conditions thereof. The bacterial strain LT3 with higher secretory volume of alkaline cellulose is obtained by steps of conducting accumulation culture, separation and purification, identification of Congo red dye and liquid fermentation on samples obtained from rotten wood or nearby soil thereof; the bacterial strain is identified to be bacillus cereus by initial identification, sequence clone and phylogenetic analysis; and 16s and r DNA sequences are shown as sequence list SEQ ID NO.1. The invention has potential theoretical and application values for research on the action mechanism of the alkaline cellulose and the production of the alkaline cellulose preparation; and the bacterial strain LT3 of the alkaline cellulose can produce the alkaline cellulose of single component and is convenient for separation and purification, thus being an ideal material for gene clone of the alkaline cellulose and having potential theoretical and practical significances for constructing alkaline cellulose fibrinolysis engineered bacterial strain and questing the action mechanism of the alkaline cellulose, and the method is simple, fast and easy for implementation.

The invention relates to an antagonistic bacterium for preventing the fungal diseases of broccoli as well as a preparation method and the application of a microbial preparation of the antagonistic bacterium, and belongs to the field of biological prevention and treatment of crop diseases. The invention provides a strain of brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622, a production method of a microbial preparation containing the brevibacillus brevis, and also the application of the brevibacillus brevis BBC-3 or the microbial preparation in preventing and treating the fungal diseases of the broccoli. The brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622 is obtained by virtue of preliminary screening and secondary screening, wherein a plate opposing test is performed for the preliminary screening and a liquid fermentation liquid is adopted for the secondary screening; the brevibacillus brevis BBC-3 is capable of effectively preventing and treating a plurality of fungal diseases of the broccoli, such as gray mold, downy mildew, stalk break, black rot and black spot, and has the prevention and treatment efficiency of above 70%; in addition, the brevibacillus brevis BBC-3 is good in environmental safety and has excellent development application prospect.

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.

The invention provides a method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu. In fermentation of the brevibacillus laterosporu, when the yield of the antimicrobial peptide is no longer increased, active carbon is directly added into the fermentation liquor; as the active carbon adsorbs the fermentation product, the inhibiting effect of the product is relieved, and the density of the thallus and the yield of the antimicrobial peptide are greatly improved. According to the method, the active carbon is taken as an adsorption stripping coupling agent, has low cost, is environmentally friendly, and can be directly added in the fermentation process without being activated in advance; the antimicrobial peptide can be adsorbed without regulating the pH value of the fermentation liquor; and the method is simple and easy to promote, and the active carbon can be regenerated through desorption after fermentation, thus being recycled repeatedly, and lowering the production cost.

The invention provides a biological water flushing fertilizer. The biological water flushing fertilizer is prepared by mixing a microorganism strain and an organic fertilizer, wherein the microorganism strain is obtained by carrying out strain liquid fermentation on bacillus subtilis, brevibacillus laterosporu and bacillus mucilaginosus; the biological water flushing fertilizer is composed of the following components in percentage by mass: 10%-20% of the microorganism strain, 20%-30% of humic acid, 30%-60% of calcium nitrate or phosphorus nitrate, 5%-10% of boric acid, 5%-12% of magnesium sulfate, 5%-8% of ferrous sulfate, 1.5%-5% of organic matters, 2%-6% of sulfur powder, 0.5%-1.0% of bentonite and 1%-5% of an inductive agent. The preparation method comprises the following steps: liquid fermentation of the strain, adsorption of fermented strain liquid, and preparation of the biological water flushing fertilizer. According to the biological water flushing fertilizer, the nutritional value of fertilizer raw materials is obviously improved and the fertilizer effect is rapidly improved; the fertilizer utilization efficiency effect and the effect of preventing diseases and increasing the output are improved. Meanwhile, the soil physical and chemical properties are also improved; the hardening is eliminated, the soil is loosened, the pH value of the soil is adjusted and the soil structure is improved.

The invention discloses a method for degrading triphenyl phosphate through brevibacillus brevis and application thereof and belongs to the technical field of environment organic pollutant biotreatment. The method specifically comprises the steps that a degradation culture medium with triphenyl phosphate as a unique carbon source is inoculated with the brevibacillus brevis under the condition that the temperature is 30 DEG C; five days after degradation of a constant-temperature shaking table, the concentration of the triphenyl phosphate left in the culture medium is measured through a gas chromatography-mass spectrometer (GC-MS), and in this way, the degrading effect of the brevibacillus brevis on the triphenyl phosphate is analyzed. According to the method, environment suitability of the method is high, and the cost is low; the degradation rate of organophosphorus fire retardant can reach 95.4% five days later. The actual application value is high, and a reference is provided for solving the problem of water organophosphorus fire retardant pollution treatment.

The invention discloses a bacillus strain for degrading aflatoxin B1 and screening and application thereof. The class of the strain is named bacillus aryabhattai DT and perserved in the China GeneralMicrobiological Culture Collection Center, with a perservation number of CGMCC 14949. A treatment method comprises: strain screening and separation by using a medium taking coumarin as a unique carbonsource, and 16S rDNA authentication. Meanwhile, the degradation effect of the fermentation culture solution of the strain on aflatoxin B1 is explored. A single colony of bacillus aryabhattai on a solid medium is picked and inoculated in 50mL of growth medium and then vibration is performed for 12-24h on a shaking table at 30-40 DEG C; the fermentation culture solution of the bacillus aryabhattaireacts with 2mu g / mL aflatoxin B1 for 3-4d in a dark place to realize a degradation rate of 82.98%. The screened bacillus aryabhattai realizes an obvious degradation effect on aflatoxin B1 and has thecharacteristics of high efficiency, energy saving, environmental friendliness and the like, and can be applied to the preparation of relevant aflatoxin detoxification agents.

The invention provides a method for producing a microbial fertilizer by using kitchen waste. The microbial fertilizer contains one selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus megatherium, Brevibacillus laterosporus, Bacillus thuringiensis, Bacillus subtilis and Streptomyces microflavus or mixed bacteria of two or more selected from the group. The method for producing the microbial fertilizer comprises the following steps: removing foreign matters in the kitchen waste, heating the kitchen waste, taking out grease, crushing the rest kitchen waste, adding enzyme for enzymolysis, putting the kitchen waste having undergone enzymolysis into a fermentation tank, performing sterilizing, inoculating one or more culturedstrains, performing fermenting with a pure bacterium or mixed bacteria to obtain a single-bacterium liquid microbial fertilizer or a composite-bacteria liquid microbial fertilizer, and adsorbing the liquid bacterial fertilizer with a carrier to obtain the solid microbial fertilizer. The microbial fertilizer is produced by utilizing the kitchen waste, so production cost is remarkably reduced, and the pollution of the kitchen waste to the environment can be effectively reduced.

The invention discloses bacillus methylotrophicus BMF 04, further discloses application of the bacillus methylotrophicus BMF 04 to inhibiting plant pathogenic fungi, application of sterile fermentation liquor of strains to inhibiting the plant pathogenic fungi, application of the sterile fermentation liquor of the strains to preparing bacterium inhibition medicines and application of the sterile fermentation liquor to promoting growth of cucumber seedlings, discloses a preparation method for sterile fermentation liquor of the bacillus methylotrophicus BMF 04 and a fermentation culture method for producing bacterium inhibition substances and further discloses a solid-state fermentation culture method for producing spores from the bacillus methylotrophicus BMF 04. A preservation number of the bacillus methylotrophicus BMF 04 is CCTCC NO:2017375. The sterile fermentation liquor of the strains is used as an effective component for the bacterium inhibition medicines. The bacillus methylotrophicus BMF 04, the application, the preparation method, the fermentation culture method and the solid-state fermentation culture method have the advantages that the novel marine bacterium strains withbroad-spectrum resistance are screened pertinently for common pathogenic bacteria in crops, and species relations of the excellent resistant strains are determined by means of strain morphological observation, physiological and biochemical tests and 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis; research is further carried out on antibacterial application of the bacillus methylotrophicus BMF 04, and directions can be provided to application of the strains.

The invention discloses a bacillus subtilis-coded PRPP (Phosphoribosyl Pyrophosphate) transamidase mutant gene pruF and an application thereof. The bacillus subtilis-coded PRPP transamidase mutant gene pruF sequence is shown as SEQ ID No.1. An engineering bacterium which is established and contains the bacillus subtilis-coded PRPP transamidase mutant gene pruF is biologically safe and clear in genetic background, so that the capacity of the bacillus subtilis which synthesizes riboflavin can be greatly improved, and the accumulation level of riboflavin can be improved by over 20%.

The invention discloses a brevibacillus brevis strain and application thereof. The strain is brevibacillus Bb-1, and is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC NO.12365. The invention also provides application of an antibiological inoculants prepared from the brevibacillus brevis Bb-1 to preparation and treatment of root-knot nematode. The brevibacillus brevis is separated from soil, has a good prevention effect on the root-knot nematode, can easily realize the fixed planting in the soil, and is favorable for the early stage prevention and control of the root-knot nematode harm. The strain has huge application potential on the problem solving of the prevention and the treatment of the root-knot nematode, and is favorable for realizing the high-yield safe and harmless production of vegetables; the killing rate of the fermentation liquid of the brevibacillus brevis Bb-1 strain on the root-knot nematode reaches 100 percent; the prevention and treatment effect in the potted plant experiments reaches 81.6 percent; the prevention and treatment effect is high and stable; the agricultural production requirements are met.

The invention discloses a blocking remover and a preparation method thereof. The blocking remover comprises following raw materials: Brevibacillus borstelensis biosurfactant fermentation liquid and hydrophobic nano silicon dioxide, and an emulsifier, an anti-scaling agent, an anti-swelling agent and a cosolvent can also be contained. The preparation method of the blocking remover comprises the following steps: firstly, adding the biosurfactant fermentation liquid, the emulsifier and the cosolvent into a reaction container, heating, and uniformly stirring; secondly, adding the anti-scaling agent and the anti-swelling agent into the solution obtained in the former step, and stirring for dissolving; and finally, continuously heating a prepared solution, adding hydrophobic inorganic nanoparticles into the reaction container, and uniformly stirring. The blocking remover has low oil-water interfacial tension, can effectively strip and emulsify residual oil and colloidal asphaltene deposits in a medium-low permeable layer to form a low-viscosity emulsion, remove organic blocking, resist hypersalinity, can be completely degraded by microorganisms, and is green and environment-friendly.