65 results about "Brevibacillus" patented technology

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention provides a bacillus strain which is named as bacillus sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37528, and the preservation date is January 26, 2026. The bacillus strain is named as bacillus sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC). The 16S rRNA (ribosomal Ribonucleic Acid) nucleotide sequence of the bacillus sp. Is as shown in SEQ ID NO: 1. Applications are also provided for promoting plant growth and improving plant quality. The bacillus sp has the capabilities of dissolving phosphorus, secreting IAA (Indoleacetic Acid) and producing siderophores, can promote plant growth and improve plant quality, and is used for promoting the growth of new wheat straw seedlings.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention relates to the field of microorganisms and application, in particular to brevibacillus mat, a microbial agent containing the brevibacillus mat and application of the brevibacillus mat. The invention provides a brevibacillus sschleri strain KY987 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the brevibacillus sschleri strain KY987 is CGMCC No. 37625. The strain not only has the activity of killing main pathogenic bacteria of soybean root rot, but also has broad-spectrum bactericidal activity on various plant pathogenic bacteria. In addition, the strain also has caenorhabditis elegans killing activity, is easy to grow, and is suitable for realizing large-scale industrial application.

This disclosure relates to compositions and methods for controlling personal care odors. More specifically, this disclosure relates to compositions comprising a ferment extract of Brevibacillus laterosporus or a fraction thereof for controlling body odor and methods for using the same. Compositions containing a ferment extract of Brevibacillus laterosporus are suitable for use as underarm / axillary deodorants and for odor control in personal care applications.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid based on a corynebacterium glutamicum CPSPC system as well as a construction method and application of the genetically engineered bacterium. Wild type C.glutamicum ATCC 13032 is taken as a chassis strain, and a functional combination of a glycine ribosome switch BsGR from bacillus subtilis and a promoter Ptuf / Ph36 is verified through fluorescent protein mCherry; a GCS system of escherichia coli and bacillus subtilis is introduced to the strain DPAg-15, genes related to synthesis of 5, 10-dimethyltetrahydrofolic acid are tested, it is found that serA delta197GTG / ATG significantly increases the yield of D-pantothenic acid, the yield of D-pantothenic acid of the constructed strain DPAj-2-tuf is increased to 23.06 g / L (63 h), the yield is 0.37 g / L / h, and the conversion rate is 0.17 g / g; the engineering strain DPAj-2-tuf is obtained by dynamically regulating and controlling a D-pantothenic acid synthesis path through a CPSPC system and combining plasmid overexpression serA delta197GTG / ATG, the yield of the engineering strain DPAj-2-tuf is increased by 2.1 times compared with that of a traditional method, and an efficient technical scheme is provided for producing D-pantothenic acid through a microbial fermentation method.

The invention relates to a complex microbial inoculant for mixed compost of sugarcane filter mud and sugarcane leaves and an application method, and belongs to the technical field of microorganisms, the complex microbial inoculant comprises a high-temperature microbial inoculant, and the high-temperature microbial inoculant is prepared from thermophilic carbon monoxide streptomyces G-4-7, brevibacillus brevis G-5-2 and thermophilic chaetomium; the medium-temperature microbial inoculum is prepared from trichoderma longibrachiatum, staphylococcus warneri LH-403 and bacillus thuringiensis LMU-81, and the medium-temperature microbial inoculum is prepared from the trichoderma longibrachiatum and the staphylococcus warneri LH-403; the complex microbial inoculant adopts a two-stage inoculation method. The complex microbial inoculant can effectively enhance degradation of lignocellulose so as to promote compost maturity, the humification degree of compost is further improved through the biological enhancement effect, the nitrogen fixation effect of the compost is facilitated, the germination index of the compost is improved, resource utilization of organic matter rich in lignocellulose can be promoted, and the environment-friendly effect is achieved. And a technical basis is provided for a green treatment mode of agricultural wastes.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The application relates to the technical field of mold removers, in particular to a preparation method of a composite mold remover and the composite mold remover. The preparation method of the composite mold remover comprises the following steps: S1. preparing modified montmorillonite; S2. culturing Brevibacillus parvus in a culture medium, obtaining Brevibacillus parvus bacterial liquid after the culturing is completed, and then preparing the obtained bacterial liquid into dry powder; S3. mixing methyl mercaptoacetate, dimethyl trisulfide and 3-methyl butyl sulfide S-methyl ester with water, and then adding the dry powder to obtain a mixed solution; S4. mixing the mixed solution with the modified montmorillonite to obtain a composite mold remover preparation; and S5. drying and packaging the composite mold remover. The composite mold remover obtained by the application is applied to moldy feed, and excellent mold removal effect can be achieved after 24 hours of mold removal treatment.

The present disclosure provides a Paenibacillus strain, and a hemostatic polysaccharide produced by same and use thereof. In the present disclosure, a Paenibacillus sp. 1229 has a deposit number of CCTCC NO: M 2022553. A produced exopolysaccharide (EPS) Hemoadhican has a hemostatic function, and can be prepared as a hemostatic material in different dosage forms for different types of hemostasis. The hemostatic material has a high hemostatic speed, no need for applying pressures to the wound for a long time, safety and non-toxicity, desirable biocompatibility, and no requirement of removal after use. The material is suitable for the application of hemostasis in irregular bleeding such as solid organs in vitro or in vivo and diffuse bleeding.

The invention discloses a culture optimization method of brevibacillus laterosporus and application of brevibacillus laterosporus in inhibiting pathogenic bacteria of hawthorn, an LB liquid culture medium is adopted in the culture process, and the culture medium comprises the following components: 10 g / L peptone, 15 g / L sucrose and 10 g / L yeast powder; the initial pH of fermentation is 7-8, the culture temperature is 30-35 DEG C, the rotating speed is 180-200rpm, the culture time is 24-36 hours, and the inoculum size of the brevibacillus laterosporus CB-528 bacterial liquid is 3-5vol%, and finally obtaining the fermented bacterial liquid through fermentation. The zymophyte liquid prepared on the basis of the method can be used for preparing biopesticides for preventing and treating Chinese yam diseases. The fermentation process disclosed by the invention is simple to control, easy to operate and obvious in effect, and the thallus yield and the spore rate can be greatly improved under the fermentation process. The fermentation culture solution disclosed by the invention has the characteristics of high activity, high viable organism content and high spore rate, and can prolong the preservation time of a subsequent preparation and improve the drug effect stability of the preparation.

This invention discloses a plasmid for gene overexpression in non-pattern *Bacillus laterosporus*. Its leader region contains a methyltransferase expression cassette derived from the *Bacillus laterosporus* i83 genome, enabling stable existence in specific hosts containing the RM system. Based on this, a PidR2 promoter is added to obtain the pMCPidR2 shuttle plasmid. The PidR2 promoter effectively drives target gene expression, the pWV01 replicon ensures plasmid replication in non-pattern hosts, and the chloramphenicol resistance gene is used for positive colony selection. The plasmid contains a TraJ element, allowing it to be introduced into non-pattern microbial hosts via conjugation transfer. This invention also provides a target gene expression strategy in non-pattern microorganisms, achieving high transformation efficiency and high strain yield through conjugation transfer transformation of donor bacteria, conjugation transfer helper bacteria, and recipient bacteria, resulting in stable expression of the target protein. This invention provides an effective vector for efficient and stable gene overexpression in specific non-pattern microorganisms, helping to solve the problem of difficult genetic manipulation of industrial production strains.

The invention relates to a thermophilic carbon monoxide streptomyces and a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the classification name of the strain is thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The efficient lignin degradation complex microbial inoculant can be prepared from the thermophilic carbon monoxide streptomyces, Brevibacillus agri G-5-2 and Chaetomium thermophilum by using the thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The complex microbial inoculant can efficiently degrade bagasse lignin in a high-temperature environment, enzyme systems of all strains are complementary, the degradation efficiency is synergistically improved, resource utilization of bagasse rich in lignin can be promoted, and a technical basis is provided for a green treatment mode of agricultural waste.

The invention belongs to the technical field of gene engineering, and relates to a plasmid of bacillus, an artificial recombinant vector as well as a construction method and application thereof, the plasmid is called pBso for short, and the nucleotide sequence of the plasmid is shown as SEQ ID NO.1. In a plasmid sequence, a protein coded by an open reading frame is a replication element Rep protein of a gram-positive bacterium rolling circle replication type plasmid, and the nucleotide sequence of the protein is shown as SEQ ID NO.2. The artificial recombinant vector is constructed by carrying out gene recombination on plasmids of the bacillus. The construction method of the artificial recombinant vector comprises the following steps: cloning a replication element Rep protein by using a molecular cloning means to construct the artificial recombinant vector with neomycin resistance. The replication element Rep protein of the plasmid pBso of the bacillus can be used for constructing an artificial recombinant vector, stably exists in the bacillus subtilis and the bacillus amyloliquefaciens and is subjected to replication and passage, and the steps of obtaining the engineering bacillus are simplified.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

U I T T R E K S E L The present invention belongs to the field of agricultural microbial control technology, and specifically relates to a growth—promoting and disease—preventing compound microbial inoculant, a preparation method and an application thereof. The compound microbial inoculant specifically includes microbial powder, growth auxiliary materials, and. a desiccant. The microorganisms include Talaromyces flavus, Trichoderma harzianum, Bacillus velezensis, Bacillus licheniformis, and Brevibacillus laterosporus. Through the synergistic effect of multiple microbial strains, the compound microbial inoculant of the present invention enhances the control effect against Verticillium wilt pathogens, and overcomes the shortcomings of a single biocontrol microbe, such as a single biocontrol mechanism, poor environmental adaptability, inability to colonize in soil for a long time, and unstable control effect. Furthermore, the compound microbial inoculant of the present invention can increase the yields of cotton and pepper, and improve the fiber quality of cotton. The present invention provides scientific and technological support and decision-making services for realizing green, environmentally friendly, cost—saving, and efficiency-increasing modern agriculture.

The invention discloses brevibacillus and application thereof in prevention and treatment of meloidogyne incognita. The brevibacillus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35054. The strain has a strong biocontrol effect on meloidogyne incognita, also has a strong growth promoting effect on tomato plant growth, and can make tomatoes blossom and bear fruits in advance.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The application discloses a soil Brevibacillus with ammonia oxidation capacity, an ammonia assimilation-ammonia oxidation composite microbial agent and application thereof. The strain is QN6, which has been preserved in the China General Microbiological Culture Collection Center on December 23, 2024, and the preservation number is CGMCC No.33138. The strain can efficiently convert ammonia nitrogen, increase the total nitrogen content and humic acid content of compost, and accelerate humification. The strain and compost bacillus QY6 (CGMCC No.33139) can be compounded into a double-function composite microbial agent to strengthen the ammonia assimilation and ammonia oxidation paths of compost, and provide an important technical breakthrough for solving the dual difficulties of 'difficult to preserve nitrogen and weak to promote decomposition' of compost. The strain and the double-function composite microbial agent can be used for composting, and the obtained compost product can significantly improve the soil nutrient supply capacity, promote crop growth and improve crop quality, and provide an effective technical means for agricultural waste resource utilization, soil improvement and high-quality crop cultivation.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

This application relates to the field of metabolic engineering and genetic engineering technology for production, specifically providing a uracil-producing strain, its construction method, and its application. This strain is... E. coli Knockout of the uridine kinase gene in the W3110 genome udk Simultaneously integrates the T7 RNA polymerase gene; weakens the uridine kinase gene. pyrH Overexpression of carbamoyl phosphate synthase gene carAB ribose-phosphokinase gene prsA pyrimidine-5'-nucleotide nuclease gene ppnN UMP phosphohydrolase gene umpH and umpG Ribonucleotide hydrolase 1 gene rihA and glutamine synthase gene glnA Heterogeneous introduction of wild-type Bacillus subtilis B. subtilis 168 orotate nucleoside monophosphate decarboxylase gene pyrF and orotic acid ribosyltransferase gene pyrE To achieve safe, efficient, and low-cost production of uracil.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

The application provides a Brevibacillus parabrevis GBW-M0 and application thereof in aquaculture. The Brevibacillus parabrevis GBW-M0 is classified as Brevibacillus parabrevis Brevibacillus laterosporus , and the preservation number is CGMCC No. 32335. The Brevibacillus parabrevis GBW-M0 is prepared through seed liquid preparation and submerged fermentation to obtain Brevibacillus parabrevis GBW-M0 fermentation liquor. The Brevibacillus parabrevis GBW-M0 fermentation liquor can reduce the concentration of hepatointestinal ciliates carried in the Penaeus vannamei, and is used for preventing and controlling microsporidian diseases of aquaculture animals. Meanwhile, the Brevibacillus parabrevis GBW-M0 fermentation liquor can inhibit the growth of Microcystis aeruginosa, and is used for treating blue-green algae blooms in aquaculture water bodies, thereby improving the success rate of aquaculture.

The application provides a huperzine A-producing cold-tolerant Paenibacillus sp. strain Sys-Hup-008, the strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, and the preservation number is CCTCC NO:M20252403; the 16S rDNA sequence of the strain is shown as SEQ ID NO. 1. The cold-tolerant Paenibacillus sp. strain Sys-Hup-008 provided in the application can metabolically produce huperzine A, can be subjected to large-scale fermentation culture in a short period, and has low fermentation cost, and is not limited by time domain, season and other conditions, so that the problems of high cost, complicated steps and many intermediate pollutants in chemical synthesis of huperzine A can be solved through the microbial fermentation approach. Meanwhile, the application also provides optimized microbial fermentation conditions and a formula of a culture medium, and the yield of huperzine A is high.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention relates to the technical field of microorganisms, in particular to a microbial composition, a complex microbial inoculant and application. The microbial composition disclosed by the invention comprises bacillus amyloliquefaciens, brevibacillus brevis and trichoderma harzianum, and can be used for simultaneously preventing and treating rice blast in a rice seed production process and aspergillus flavus pollution in a storage process, so that the full life cycle protection of the rice seeds from production to storage is realized; the problem that in the prior art, different agents need to be used for preventing and treating rice blast and aspergillus flavus pollution is solved. In addition, the complex microbial inoculant containing the microbial composition is safe, efficient and environment-friendly, can replace chemical pesticides, reduces environmental pollution and pesticide residues, is simple in preparation method and easy for industrial production, and has a wide application prospect.