317 results about "Brevibacillus" patented technology

Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

The invention provides a kind of Brevibacillus laterosporus (STP-B.latCGMCCNo.1119), the character follows this. G+, haulm shape, the shape is (0.4-0.5)*(2-2.5)mu. The spore adnation is from neutrality to sub-neutrality. The spore bursa will enlarge. Most of the spore is vertebra aitch, the remainder is disciform. VP-, ph value of culture fluid is 7.2, amylolysis-, gelatin dispergation-, decomposition casein+, katalase+, oxidase-, cirate -, dextrose aerogenesis-, dextrose acidgenesis+, L-arabinose acidgenesis-, xylose acidgenesis-, mannit acidgenesis+, azotate deocidisation+, methyl red+, pH5.7 growth+.

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

The present invention belongs to the technical field of environment protection, in particular to a method to degrade Microcystic aeruginosa through microorganisms. The method is that firstly an algae-lysing bacterial strain Brevibacillus spp.FDK2 can be achieved by separating from and sublimating deep eutrophication urban lake water body;the algae-lysing bacterial strain Brevibacillus spp.FDK2 is cultured by a culture medium to acquire bacterial liquid with a certain concentration. The bacterial liquid is added into a water sample containing Microcystic aeruginosa. Under a certain condition, the bacterial strain has a good degrading effect on the Microcystic aeruginosa. The degrading removing rate is more than 90 percent.

The invention discloses a bacillus strain for degrading aflatoxin B1 and screening and application thereof. The class of the strain is named bacillus aryabhattai DT and perserved in the China GeneralMicrobiological Culture Collection Center, with a perservation number of CGMCC 14949. A treatment method comprises: strain screening and separation by using a medium taking coumarin as a unique carbonsource, and 16S rDNA authentication. Meanwhile, the degradation effect of the fermentation culture solution of the strain on aflatoxin B1 is explored. A single colony of bacillus aryabhattai on a solid medium is picked and inoculated in 50mL of growth medium and then vibration is performed for 12-24h on a shaking table at 30-40 DEG C; the fermentation culture solution of the bacillus aryabhattaireacts with 2mu g / mL aflatoxin B1 for 3-4d in a dark place to realize a degradation rate of 82.98%. The screened bacillus aryabhattai realizes an obvious degradation effect on aflatoxin B1 and has thecharacteristics of high efficiency, energy saving, environmental friendliness and the like, and can be applied to the preparation of relevant aflatoxin detoxification agents.

The invention discloses bacillus methylotrophicus BMF 04, further discloses application of the bacillus methylotrophicus BMF 04 to inhibiting plant pathogenic fungi, application of sterile fermentation liquor of strains to inhibiting the plant pathogenic fungi, application of the sterile fermentation liquor of the strains to preparing bacterium inhibition medicines and application of the sterile fermentation liquor to promoting growth of cucumber seedlings, discloses a preparation method for sterile fermentation liquor of the bacillus methylotrophicus BMF 04 and a fermentation culture method for producing bacterium inhibition substances and further discloses a solid-state fermentation culture method for producing spores from the bacillus methylotrophicus BMF 04. A preservation number of the bacillus methylotrophicus BMF 04 is CCTCC NO:2017375. The sterile fermentation liquor of the strains is used as an effective component for the bacterium inhibition medicines. The bacillus methylotrophicus BMF 04, the application, the preparation method, the fermentation culture method and the solid-state fermentation culture method have the advantages that the novel marine bacterium strains withbroad-spectrum resistance are screened pertinently for common pathogenic bacteria in crops, and species relations of the excellent resistant strains are determined by means of strain morphological observation, physiological and biochemical tests and 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis; research is further carried out on antibacterial application of the bacillus methylotrophicus BMF 04, and directions can be provided to application of the strains.

The invention discloses a bacillus subtilis-coded PRPP (Phosphoribosyl Pyrophosphate) transamidase mutant gene pruF and an application thereof. The bacillus subtilis-coded PRPP transamidase mutant gene pruF sequence is shown as SEQ ID No.1. An engineering bacterium which is established and contains the bacillus subtilis-coded PRPP transamidase mutant gene pruF is biologically safe and clear in genetic background, so that the capacity of the bacillus subtilis which synthesizes riboflavin can be greatly improved, and the accumulation level of riboflavin can be improved by over 20%.