38 results about "L-Glucose" patented technology

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

The invention relates to the technical field of gene engineering, in particular to a D-psicose-3-epimerase mutant and application of the D-psicose-3-epimerase mutant in synthesis of psicose. According to the present invention, the 59th site, the 106th site and the 188th site of the wild type D-psicose-3-epimerase derived from Brevibacillus thermophilus (Brevibacillus thermophilus) are subjected to simultaneous mutation to obtain the D-psicose-3-epimerase mutant, such that the efficiency of producing D-psicose by using fructose as the raw material is significantly improved, the reaction of catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the yield of the D-psicose is significantly improved; the conversion rate is up to 36.5%; according to the present invention, the coexpression binding enzyme strain is constructed by coupling glucose isomerase, such that the intermediate transfer efficiency and the enzyme stability are improved, the reaction for producing D-psicose through catalysis of 500 g / L glucose is performed for 6 h, and the conversion rate can achieve 30.5%; and the activity of the immobilized enzyme can still be kept at 80% after the immobilized enzyme is repeatedly used for 10 batches.

The invention discloses a beta-glucosidase mutant and application of the beta-glucosidase mutant in preparation of gentiooligosaccharide, and belongs to the technical field of enzyme engineering and functional oligosaccharide preparation. According to the invention, site-directed mutagenesis is carried out on a beta-glucosidase gene derived from Thermotoga maristima STB25, and high-efficiency expression of the beta-glucosidase gene in bacillus subtilis is realized. Beta-glucosidase prepared from the mutant is added into a reaction system with 480 g / L glucose as a substrate, reaction is carried out under the conditions that the pH is 6.0 and the temperature is 60 DEG C, and the yield of gentiobiose oligomer can reach 22.12% and is increased by 66.44% compared with that of a wild type. The beta-glucosidase mutant provided by the invention has good acid stability, thermal stability and glucoside conversion capability, can generate gentiobiose through catalysis by taking glucose as a substrate, and has a relatively good application prospect in the field of gentiooligosaccharide production.

The invention discloses a construction method of engineering bacteria for producing glycerol and lipid by efficiently fermenting lignocellulose hydrolysate, and belongs to the technical field of microorganisms. The invention provides a method for producing glycerol and lipid by fermenting lignocellulose hydrolysate by using Candida glycinogenes which is resistant to high osmotic pressure as a production strain. According to the present invention, with the Candida glycyinogenes engineering strain Cg-AAGD constructed by using the method, the virus-free lignocellulose hydrolysate (containing about 175 g / L of glucose and 25 g / L of xylose) can be fermented to produce 82.2 g / L of glycerol and 7.3 g / L of lipid; according to the method, the lignocellulose hydrolysate is selected as a raw material, the purpose of deep resource utilization of agricultural waste is achieved, the related technological operation flow is relatively simple and convenient, and the requirement for production equipment is easy to achieve.

The invention relates to a culture medium for serum-free suspension culture of F81 cells and application of the culture medium, the culture medium comprises the following components in concentration: 4000-4500 mg / L of amino acid, 80-120 mg / L of vitamin, 5000-5100 mg / L of inorganic salt, 5000-5500 mg / L of glucose, 1000-1030 mg / L of adherence promoting substance, 20-40 mg / L of growth factor, 2000-2040 mg / L of buffering agent and 2-2.4 mg / L of antioxidant, the pH value of the culture medium is 7.0-7.2, and the pH value of the culture medium is 0.5-1.5. The antioxidant is prepared from partially hydroxylated glutathione and a carboxymethyl chitosan-gallic acid grafted copolymer. The partially hydroxylated glutathione and the carboxymethyl chitosan-gallic acid graft copolymer are compounded to serve as an antioxidant of the culture medium, so that the oxidation resistance of the culture medium is enhanced synergistically, and the cell survival rate is increased.

The invention is applicable to the technical field of microbial fermentation, and provides a use method of an erythritol fermentation accelerant, which comprises the following steps: step 1, culturing to obtain a candida lipolytica seed solution; step 2, fermenting erythritol for 22-26 hours in the first stage; the fermentation medium comprises 10-12 g / L of a fermentation accelerator, and the fermentation accelerator comprises 20-22 parts of urea; 30 to 40 parts of trehalose; 1.4 to 1.6 parts of inositol; and step three, at the second fermentation stage of the erythritol, pumping 130-160g / L glucose into the fermentation tank at one time until the fermentation total sugar concentration is 300-320g / L, and continuing fermentation to obtain the erythritol. In conclusion, by adding the fermentation accelerant, the endurance capacity and the metabolic activity of the thalli to the hypertonic environment and the activity of key enzymes such as erythritol reductase are improved, meanwhile, after the thalli enter the logarithmic phase, glucose is supplemented till the thalli are stressed to produce sugar alcohol with high concentration and hyperosmotic pressure, the fermentation period can be effectively shortened, and the erythritol conversion rate is improved.

The invention discloses a screening method of high-yield glycerol saccharomyces cerevisiae, and belongs to the technical field of microorganisms. The screening method comprises the following steps: (1) respectively preparing a liquid culture medium containing 200-350g / L of glucose and a solid culture medium containing 200-350g / L of glucose; (2) inoculating fermented mash in a fermentation stage after yellow wine brewing into a liquid culture medium, and culturing; then carrying out gradient dilution on the culture solution to obtain a bacterial suspension; coating the bacterial suspension on a flat plate of a solid culture medium, and standing and culturing; (3) picking bacterial colonies from the solid culture medium, inoculating the bacterial colonies into a liquid culture medium, and culturing to complete strain activation; inoculating the activated strain into a new liquid culture medium for culturing and fermenting; taking fermentation liquor, determining the content of glycerol by adopting a high performance liquid chromatography, and screening to obtain the high-yield glycerol strain. According to the invention, strains for efficiently synthesizing glycerol can be screened in a short time, and the workload of screening high-yield glycerol strains is effectively reduced.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The invention discloses a low-temperature preservation method of Octopus variabilis sperms and spermatophore, the Octopus variabilis sperms adopt an ultralow-temperature cryopreservation method, the Octopus variabilis sperms are protected by using a cryoprotectant composed of a 0.6 mol / L glucose solution diluent and 20% glycerol, the diluent contains nutrient substances, and the Octopus variabilis sperms have the characteristics of inactivation of sperms and small damage to sperms. The cryoprotectant has the functions of a permeable antifreeze agent and a non-permeable antifreeze agent, so that the sperms are subjected to dual protection in the freezing process; the octopus variabilis spermatophore is preserved at a low temperature, a 1.0 mol / L glucose solution is used as a spermatophore protection solution, the spermatophore structure can be maintained within one month, and high sperm activity of octopus variabilis sperms can be maintained within a short period (one month). Based on requirements in experiments, two octopus variabilis sperm preservation methods are provided and can be flexibly used, and technical support is provided for preservation of octopus variabilis germplasm resources and overcoming of the problem of asynchronous male and female development.

The invention discloses a method for improving biosynthesis of propylene glycol by thermoanaerobacter thermosaccharolyticus through metabolic engineering modification, and belongs to the technical field of genetic engineering. According to the invention, the tdk gene, the adhE gene and the Ldh gene are knocked out, so that the propylene glycol synthesis capability of the thermoanaerobacter thermosaccharolyticus is improved, the fermentation yield of propylene glycol is further improved, and compared with an original strain, when domestication fermentation is carried out by taking 10g / L glucose as a substrate, the propylene glycol yield of a wild type strain is 1.07 g / L, the propylene glycol yield of a modified engineering strain is 3.95 g / L, and the propylene glycol yield is improved by 269.2%. In the fermentation test process by increasing the glucose concentration, the yield of propylene glycol consumed by 29.5 g / L glucose can reach 11.62 g / L, the conversion rate from glucose to propylene glycol reaches 39.4%, and the method has industrial application potential.

The invention relates to a serum-free culture medium for in-vitro amplification of neural stem cells and a preparation method of the serum-free culture medium, and belongs to the technical field of biology. The culture medium comprises a DMEM / F12 and Neurobasic double-basal culture medium (the volume ratio is 1: 1), a bFGF / EGF / BDNF three-growth-factor combination (20 / 15 / 10ng / mL), a B27 / N2 supplement and a metabolism regulator (6g / L glucose + 0.1 mM beta-mercaptoethanol), and is prepared through the processes of stepwise dissolution of a buffer system, magnetic stirring and mixing, positive-pressure filtration and sterilization and the like. The innovation points are as follows: the double-basal culture medium synergistically balances amino acid and neurotransmitter nutrition, so that the glucose consumption rate is reduced by 25%; the three factors synergistically activate a proliferation pathway and inhibit differentiation, the amplification multiple reaches 14.8 times (7 days), and the Netin positive rate is gt; 95%; a metabolism regulation system controls lactic acid lt; the karyotype abnormality rate is 1t; and 0.3%. According to the scheme, the cost is reduced by 33%, and the method is completely free of animal-derived components and suitable for large-scale automatic production of clinical-grade neural stem cells.

The invention provides a strain for producing mannitol and a method for producing mannitol by using the strain, and belongs to the technical field of food biology. According to the present invention, the fermentation lactobacillus fermentum SK50.004 is screened from the pickled vegetables, and the strain is used for the fermentation production of the mannitol; by optimizing the carbon source composition and inorganic salt components in the fermentation medium, the yield of mannitol produced by fermentation of the strain at 37-40 DEG C under the conditions that the concentration of CuSO45H2O is 10 mg / L and the total sugar concentration is 265 g / L (glucose is 80 g / L and fructose is 185 g / L) reaches 160 g / L, the maximum productivity is 4.21 g / L / h, byproducts in the fermentation process are reduced, the production efficiency is remarkably improved, and the production cost is reduced. And a reliable technical support is provided for industrial efficient production of mannitol.

The present invention discloses a chemically defined culture medium for producing outer membrane vesicles and its use. Specifically, the chemically defined culture medium of the present invention comprises: 0.75-12 g / L ammonium sulfate, 2.5-20 g / L sodium chloride, 0.75-12 g / L sodium hydrogen phosphate, 0.75-12 g / L sodium thiosulfate pentahydrate, 2.5-40 g / L glucose, 0.075-1.2 g / L glutamic acid, 0.075-1.2 g / L arginine, 0.075-1.2 g / L serine, 0.001-0.02 g / L vitamin B5, 0.001-0.02 g / L vitamin B6, 0.01-0.2 g / L calcium chloride dihydrate, 0.03-0.4 g / L magnesium sulfate, and 0.003-0.04 g / L ferrous sulfate heptahydrate. The chemically defined culture medium of the present invention is safe, environmentally friendly, and can produce a high yield of outer membrane vesicles.

The present invention discloses an enzymatic synthesis system and application of a levonorgestrel intermediate, comprising the following components: 1.800-5.900 g / L dipotassium hydrogen phosphate, 0.775-2.325 g / L potassium dihydrogen phosphate, 38.500-115.500 g / L glucose, 0.100-0.188 g / L Tween 600.100, 13.500-40.500 g / L Triton X-100, and 0.050-0.150 g / L defoamer. The reaction catalytic system of the present invention ensures substrate emulsification while reducing product emulsification, significantly promoting reaction conversion and increasing the yield of the reaction product by as much as 95%.

The present invention relates to the technical field of cell culture, and specifically discloses a method for domesticating an adherent cell line into a fully suspended cell line. The adherent-dependent cells are inoculated on microcarriers and cell proliferation is carried out in DMEM medium containing 10% fetal bovine serum. After the cells proliferate to 10E6 cells / mL, the microcarriers are lysed, the cell clusters are centrifuged and collected. The cell clusters are inoculated in a medium containing 10% fetal bovine serum, 0.1% shear force protectant, and 2 g / L glucose and cultured at a rotation speed of 30 to 50 rpm for 3 to 4 days to enable the cells to form embryoid body clusters of 50 μm to 150 μm. The embryoid body clusters are mechanically pipetted to disperse them, and are domesticated for 18 to 22 days with DMEM medium containing different concentration gradients of low molecular weight dextran sulfate to obtain a successfully suspended and domesticated cell line. The method of the present invention improves the uniformity of cell embryoid bodies, makes the properties of the embryoid bodies more stable, and can efficiently obtain a suspended cell line.

The present invention belongs to the field of microbial technology and discloses an enzyme mutation expression engineered bacterium for synthesizing D-psicose and its application. Thermus oshimai Glucose isomerase and its source Flavonifractor of the lungs A dual-enzyme system DH5α / pHY-P constructed by D-psicose 3-epimerase 43 ‑TogI‑P 43‑ DAEase, and through protein modification, the mutant strain BL10 / pHY-P was constructed to produce glucose isomerase and D-psicose 3-epimerase. 43 ‑TogI 182 ‑P 43 ‑DAEase 38 The strain was used for whole-cell catalysis, and it was able to catalyze the one-pot conversion of 500 g / L glucose into 185 g / L D-psicose, with a conversion rate of up to 35%, which is the highest level of D-psicose production from glucose at present. It greatly reduces production costs and has significant economic benefits.

The invention relates to the technical field of veterinary microbiology, in particular to a culture medium suitable for mycoplasma bovis culture and a preparation method of the culture medium. The culture medium is composed of a basic part and an auxiliary part, the basic part is prepared from 15-16 g / L of PPLO powder, 2-3 g / L of yeast extract, 2-3 g / L of sodium pyruvate, 1-1.5 g / L of glucose and 20-60 ml / L of phosphate buffer solution and ultrapure water (0.06-0.12 g / L of agar is added to a solid culture medium), and the auxiliary part is prepared from a proper amount of sodium chloride and a proper amount of sodium chloride. The auxiliary part comprises 1 to 1.5 ml / L of 10 * MEM, 8 to 12 ml / L of cholesterol with a mass concentration of 0.5%, 8 to 12 ml / L of glutamine with a mass concentration of 1%, 8 to 12 ml / L of crotonic acid with a mass concentration of 4%, 8 to 12 ml / L of malic acid with a mass concentration of 0.5%, 2 to 6 ml / L of a deoxynucleotide sodium salt solution with a molar concentration of 5 mmol / L, 40 to 60 ml / L of bovine serum and ultrapure water; the invention also provides a preparation method and application of the culture medium, which are simple and convenient, small in batch difference, simplified in production process and improved in product stability. According to the culture medium, stable mycoplasma bovis with high viable count can be obtained in a short time, and meanwhile, the problems of long detection period and the like of an existing quantitative bacterium concentration method can be effectively solved.

The application discloses a recombinant domain fusion enzyme and application thereof in preparation of D-allulose, wherein the recombinant domain fusion enzyme is formed by splicing and recombining a 5' domain and a partial 3' domain of D-tagatose 3-epimerase and an active center domain of xylose isomerase. The application uses the domain fusion enzyme of the 5' domain and the partial 3' domain of D-tagatose 3-epimerase and the active center domain of xylose isomerase as a biological catalyst, and the yield of D-allulose generated by catalyzing isomerization of 260 g / L glucose is 35.9%. The method disclosed by the application is rapid and efficient, reaction conditions are simple, substrate conversion rate is high, and byproducts are few, so that the pressure of separation and purification is reduced, and the method is suitable for large-scale industrial production.

The invention relates to a dendrobium officinale tender shoot extract and application thereof, in particular to application of the dendrobium officinale tender shoot extract obtained by differentiation induction of dendrobium officinale stem cells in preparation of products with whitening and / or freckle removing effects. Wherein the differentiation induction is carried out in an MS culture medium containing 0.1 to 1 g / L of NAA, 0.5 to 2 mg / L of KT, 15 to 30 g / L of glucose and 10 to 100 g / L of maltose. The extract of the dendrobium officinale tender shoots differentiated from the dendrobium officinale stem cells provided by the invention can obviously inhibit the activity of tyrosinase, so that the effects of whitening and removing freckles are realized.

The invention provides an aspergillus flavus selective culture medium, a preparation method and application thereof, and a separation method and a culture method of aspergillus flavus, and relates to the technical field of biology. The aspergillus flavus selective culture medium provided by the invention is prepared from 50 to 100g / L of corn flour, 50 to 100g / L of peanut powder, 1 to 5g / L of citric acid, 1 to 5g / L of malic acid, 15 to 20g / L of agar powder, 10 to 20g / L of glucose, 50 to 100mg / L of chloramphenicol, 100 to 200mg / L of cycloheximide, 5 to 10g / L of sodium chloride, 15 to 20g / L of dichloronaphthalene chloramphenicol agar, 2 to 5g / L of yeast extract, 5 to 10g / L of peptone, 0.5 g / L of magnesium sulfate and 1g / L of monopotassium phosphate. The pH value of the aspergillus flavus selective culture medium is 5.5-6.5. The culture medium can significantly improve the aspergillus flavus separation efficiency.

The invention relates to the technical field of edible fungus cultivation, in particular to a lentinus edodes liquid strain culture medium containing macadamia nut skin powder and application thereof.The lentinus edodes liquid strain culture medium containing macadamia nut skin powder comprises 15 g / L of corn flour, 5 g / L of wheat bran, 20 g / L of glucose, 3 g / L of yeast extract, 2 g / L of monopotassium phosphate, 1 g / L of magnesium sulfate and 5-25 g / L of macadamia nut skin powder, the mushroom liquid strain prepared by adopting the culture medium disclosed by the invention has the advantages that the pellet density is improved by 13-35%, the manganese peroxidase activity is improved by 6.67-24 times, the laccase activity is improved by 2.37-4 times, and hyphae can grow more efficiently after the mushroom liquid strain is inoculated to a culture medium.

The present invention relates to the field of genetic engineering technology, and particularly relates to a D-psicose-3-epimerase mutant and its application in the synthesis of psicose. The present invention simultaneously mutates multiple sites at the 59th, 106th, and 188th positions of the wild-type D-psicose-3-epimerase derived from Brevibacillus thermoruber Brevibacillus thermoruber to obtain a D-psicose-3-epimerase mutant, significantly improving its efficiency in producing D-psicose from fructose. When catalyzing the reaction of 500 g / L fructose to produce D-psicose for 4 h, the conversion rate reaches 36.5%. By coupling with glucose isomerase and constructing a co-expressing conjugate enzyme strain, the intermediate transfer efficiency and the stability of the enzyme are improved. When catalyzing the reaction of 500 g / L glucose to produce D-psicose for 6 h, the conversion rate reaches 30.5%. Moreover, the immobilized enzyme can be reused for 10 batches, and its activity can still be maintained at 80%.

The invention discloses saccharomyces cerevisiae capable of efficiently utilizing cellulose hydrolysate as well as a construction method and application thereof. The construction method comprises the following steps: 1) selecting a xylose metabolism saccharomyces cerevisiae haploid chassis strain of a xylose isomerase pathway; 2) performing spore splitting on industrial ethanol-producing yeast to obtain haploid industrial yeast strains; and (3) fusing and screening the strains obtained in the steps (1) and (2) to obtain the saccharomyces cerevisiae capable of efficiently utilizing the cellulose hydrolysate. The saccharomyces cerevisiae. At the shake flask level, 70 g / L of glucose in the acid-treated cellulose hydrolysate can be completely consumed within about 24 h, meanwhile, xylose starts to be consumed gradually, and 20 g / L of xylose in the acid-treated cellulose hydrolysate can be consumed within 72 h. The growth amount OD600 can reach about 15.0 at most, 45 g / L of ethanol is generated under the aerobic condition, rapid growth in industrial cellulose hydrolysate is achieved, glucose and xylose are fully utilized as growth carbon sources, and ethanol is generated.

This invention discloses a high-antigenic-activity avian Pasteurella multocida culture medium, its preparation method, and its application. The high-antigenic-activity avian Pasteurella multocida culture medium consists of: 8-13 g / L bacteriological peptone, 3-8 g / L growth-promoting peptides, 18-23 g / L yeast extract, 2-7 g / L glucose, 4-9 g / L sodium chloride, 1-5 g / L dipotassium hydrogen phosphate, and the remainder being water. Using this high-antigenic-activity avian Pasteurella multocida vaccine culture medium promotes bacterial growth and reproduction, requires a shorter culture time, and achieves a high cell density, ultimately reaching 1.84 × 10¹⁰ CFU / mL. It significantly improves fermentation speed and the cell density of avian Pasteurella multocida in the fermentation broth, making it more suitable for culturing avian Pasteurella multocida. Furthermore, the vaccine prepared using the culture medium provided by this invention has a protection efficiency of up to 100% and excellent antigenic activity, laying the foundation for the production of avian Pasteurella multocida vaccines.

The present invention relates to the technical field of cell culture. Specifically disclosed is a recombinant trypsin cell digestion solution for maintaining high cell viability. The recombinant trypsin cell digestion solution consists of the following concentrations of components: 5000-8000 mg / L sodium chloride, 500-1500 mg / L glucose, 500-1500 mg / L disodium hydrogen phosphate, 100-400 mg / L sodium bicarbonate, 1000-2000 mg / L potassium dihydrogen phosphate, 1000-2000 mg / L potassium chloride, 0.5-2 mM of EDTA, 100-500 mg / L poloxamer 188 PRO, 100-500 mg / L HEPES, and 100-500 mg / L recombinant trypsin, with the balance being pure water. The provided recombinant trypsin cell digestion solution greatly improves cell viability after digestion, and solves the problem of reduced cell viability after conventional trypsin digestion.

The present application relates to Dendrobium candidum tender bud extract and its use, specifically to the application of Dendrobium candidum tender bud extract obtained by differentiation induction of Dendrobium candidum stem cells in the preparation of products with whitening and / or freckle-removing effects; wherein the differentiation induction is carried out in MS medium containing 0.1-1 g / L NAA, 0.5-2 mg / L KT, 15-30 g / L glucose and 10-100 g / L maltose. The Dendrobium candidum tender bud extract obtained by differentiation of Dendrobium candidum stem cells provided by the present application can significantly inhibit the activity of tyrosinase, thereby achieving the effects of whitening and freckle removal.

The application discloses a kind of recombinant escherichia coli and its construction method and the method for producing glucaric acid by metabolic engineering, belong to the field of metabolic engineering.The application realizes the production pathway of glucuronic acid by expressing the myo-inositol-1-phosphate synthase gene (Ino1) and myo-inositol oxidase gene (MIOX) derived from plants in escherichia coli, simultaneously expresses the aldehyde acid dehydrogenase gene (Udh) derived from Agrobacterium tumefaciens, and successfully constructs the synthesis pathway of glucaric acid.The recombinant escherichia coli generates glucaric acid 2.53g / L in LB-G (10g / L glucose is added in LB medium) fermentation medium.The method for fermenting and producing glucaric acid by the recombinant escherichia coli proposed in the application has broad development prospect, and lays the foundation for biosynthesis of glucaric acid.

The application belongs to the technical field of hangover food after acute alcoholism, and discloses application of fermented sea cucumber liquid rich in acidic polysaccharide in hangover food. The hangover food is fermented sea cucumber liquid rich in acidic polysaccharide; the fermented sea cucumber liquid rich in acidic polysaccharide comprises 35% sea cucumber homogenate liquid, 7.5 g / L glucose and 4% probiotic strains. The fermented sea cucumber liquid rich in acidic polysaccharide activates ethanol dehydrogenase and acetaldehyde dehydrogenase activity, reduces blood alcohol concentration after 3 hours of drinking, prolongs the average latency time, shortens the average duration time and improves the tolerance to acute alcoholism; improves liver antioxidant enzyme activity and slows down liver cell damage caused by continuous intake of high-dose alcohol. The fermented sea cucumber liquid rich in acidic polysaccharide can be applied to the prevention of acute alcoholism and hangover health care functional food.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

The invention discloses a tea culture medium capable of improving antioxidant activity of lactobacillus plantarum, which is prepared by the following steps: soaking sour tea in boiling water, filtering to obtain tea water, dissolving 1 / 5-1 / 10 MRS culture medium in the tea water, and sterilizing at high temperature and high pressure, or 38-42 g / L of peptone, 18-22 g / L of glucose, 0.08-0.12 g / L of magnesium sulfate, 0.4-0.6 g / L of manganese sulfate, 1-3 g / L of dipotassium phosphate and 1 L of tea water are mixed and sterilized to prepare the culture medium. Through a DPPH clearance rate experiment, the tea culture medium disclosed by the invention is found to be capable of improving the antioxidant activity of the plant lactobacillus SC75-2-2; the method comprises the following steps: culturing the plant lactobacillus SC75-2-2 by adopting a tea culture medium, crushing a culture bacterium solution to obtain an acellular supernatant, smearing the acellular supernatant on a rat full-thickness skin wound, collecting a skin tissue sample, and carrying out HE analysis, Masson analysis and immunohistochemical analysis, and the result shows that the acellular supernatant has the capability of promoting wound healing.