120 results about "L-Glucose" patented technology

The invention relates to a medicinal preparation with inhibiting effect on tumor metastasis, which contains steroidal saponin extractives. The effective active components of the medicinal preparation comprise the following eight steroidal saponin general extractives which are eight steroid saponins: steroidal saponin I, steroidal saponin II, dioscin, gracillin, steroidal saponin H, steroidal saponin VII, saponin A (pennogenin-3-O-alpha-L-isodulcitol-yl(1-4)-[alpha-L-isodulcitol-yl(1-2)]-alpha-L-isodulcitol-yl(1-2)-beta-D-heteroside) and saponin B (pennogenin-3-O-alpha-L-isodulcitol-yl(1-4)-[alpha-L-isodulcitol-yl(1-2)]-alpha-L-isodulcitol-yl(1-3)-beta-D-heteroside), and the effective active components of the medicinal preparation also comprise medical dressings. The weight content of the eight steroidal saponin general extractives is 5%-30%, and the weight content of the medical dressings is 70%-95%. The invention is a medicinal preparation with less toxic and side effects and inhibiting effect on tumor metastasis.

The invention discloses a quantitative fructose assay kit which comprises inorganic acid deproteinized extract A, inorganic base deproteinized extract B, fructose calibration solution, a reagent 1 containing 0.001-0.1mol/L adenosine triphosphate sodium salt, a reagent 2 containing 1-100KU/L hexokinase and 1-100KU/L glucose-6-phosphate dehydrogenase, and a reagent 3 containing 0.001-0.1mol/L nicotinamide adenine dinucleotide. The seminal plasma fructose assay method comprises the following steps: respectively adding the reagent 1 and the reagent 2 to deproteinized seminal plasma and the fructose calibration solution, and mixing uniformly; reacting at the temperature of 10-40 DEG C for 5-120 minutes, then reading the absorbance respectively at the wavelength of 280-400nm; adding the reagent 3 respectively, and mixing uniformly; reacting under the same conditions and reading the absorbance; and calculating the difference between the absorbance read at the first time and the absorbance read at the second time, and comparing or calculating the absorbance of a seminal plasma specimen and the fructose calibration solution to obtain the concentration of the seminal plasma fructose. The kit and the method can be used for quantitative determination of fructose in sera, plasma, body fluid, food and solid extracting solution, the methodology is special, unique, clean and environment-friendly, manual operation and automatic batch assay can be realized, and the kit and the method are easy to popularize and apply clinically.

Methods for synthesizing Salacinol, its stereoisomers, and analogues, homologues and other derivatives thereof potentially useful as glycosidase inhibitors are described. In some embodiments the compounds of the invention may have the general formula (I) or (II):

The synthetic schemes may comprise reacting a cyclic sulfate with a 5-membered ring sugar containing a heteroatom (X). The heteroatom preferably comprises sulfur, selenium, or nitrogen. The cyclic sulfate and ring sugar reagents may be readily prepared from carbohydrate precursors, such as D-glucose, L-glucose, D-xylose and L-xylose. The target compounds are prepared by opening of the cyclic sulfates by nucleophilic attack of the heteroatoms on the 5-membered ring sugars. The resulting heterocyclic compounds have a stable, inner salt structure comprising a heteroatom cation and a sulfate anion. The synthetic schemes yield various stereoisomers of the target compounds in moderate to good yields with limited side-reactions. Chain-extended analogues of Salacinol are also described.

The invention discloses a corynebacterium glutamicum strain for production of 5-aminolevulinic acid and construction and application of corynebacterium glutamicum strain. A construction method includes: (1) deleting a lactic dehydrogenase coding gene 1dhA and acetic acid generation genes pta-ackA, pqo and cat in corynebacterium glutamicum to obtain a strain named CB4; inserting a strong sod promoter in front of a phosphoenolpyruvate carboxylase coding gene ppc in the strain CB4 to obtain a strain CB5; deleting a gene pck in the strain CB5 to obtain a strain CB6; (2) transferring plasmid pXA and plasmid pEP2<tuf>-rhtA into the strain CB6. The strain constructed according to the method is capable of generating 2.78g/L 5-aminolevulinic acid in a culture medium with 10g/L glucose serving as a carbon source, which lays a foundation for subsequent continuous feeding of a fermentation tank to increase yield of the 5-aminolevulinic acid.

The purpose of the present invention is to provide a method for highly accurately detecting a cancer cell. The method of the present invention is characterized by comprising imaging with the use of a fluorescently labeled L-glucose derivative. By using the method and imaging agent according to the present invention, a high contrast between a cancer cell and a normal cell can be obtained compared with the case that imaging is conducted with the use of a fluorescently labeled D-glucose derivative. According to this method, moreover, no fasting is needed for the determination. Thus, the imaging can be quickly carried out without imposing a burden on a patient.

A soft drink and method of producing a soft drink that is a fortified, normally zero calorie beverage, formulated with natural flavors such as lemon or berry that can be formulated from L-Gluamine, sodium bicarbonate, a potassium salt like potassium chloride or potassium gluconate, caffeine with optional B vitamins, folic acid and vitamin C added for fortification along with a sweetener. This drink mixture can help reduce or eliminate symptoms of a hangover caused by alcohol consumption as well as supply a large portion of the recommended daily requirements for some vitamins and minerals.

An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.

Adipose tissue cryopreservation liquid at a clinical application level and a cryopreservation method. The adipose tissue cryopreservation liquid at the clinical application level comprises 15-50-mmol/L saccharose, 15-50-mmol/L mannitol injection, 1-5-mmol/L glucose injection, 1-3-mol/L adenosine injection, 0.5-5-mmol/L reduced glutathione, 1-4.5-mol/L trehalose and 0.1-1-mmol/L EDTA-2Na; and the components are mixed to form mixed liquid, and the mixed liquid is the adipose tissue cryopreservation liquid at the clinical application level. By means of the adipose tissue cryopreservation liquid,in combination with the cryopreservation method thereof, at the clinical application level, the defect of adverse reaction due to 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), phenol red, DMSO, animal serum, blood platelet extracts and the like which are used in the prior art is effectively overcome.

The invention provides an isobutanol synthetic strain construction method implemented by guiding the adjustment of intracellular reducing power based on a genomic scale metabolic network model. Based on the genomic scale metabolic network model, by adopting flow balance analysis and metabolic minimum adjustment analysis, the action law of different reconstruction modes of an intracellular reducing power metabolism to strain growth and isobutanol synthesis is simulated, and according to phenotypic coefficients, a conclusion that glyceraldehyde-3-phosphate dehydrogenase is a key target spot of the intracellular reducing power adjustment of an isobutanol synthetic strain is obtained. By using a synthetic biological artificially-regulated element, an NADP+ depended glycerin-3-phosphate dehydrogenase metabolic pathway is built and adjusted so as to match and balance the intracellular reducing power metabolism, thereby obtaining an efficient isobutanol synthetic strain. The intracellular NADPH/NADP ratio of the strain reaches 0.4-0.8, and when 20-50 g/L glucose as a substrate is adopted for carrying out batch fermentation, the yield of isobutanol can reach over 8 g/L in 36 h, which is increased by over 60%.

The invention provides a kit for extracting DNA (deoxyribonucleic acid) from white cells in human whole blood. The kit comprises a red blood cell lysis buffer and a DNA extracting solution, wherein the red blood cell lysis buffer comprises the following components: 4.5-5.5mmol/L sodium chloride, 0.8-1.2 percent (v/v) triton 100, 300-340mmol/L glucose and 0.008-0.012mol/L tri-hydroxymethyl aminomethane. The kit provided by the invention can be used for solving the defect of an existing kit for extracting the DNA in human whole blood white cells. The invention provides a kit for quickly extracting the DNA from white cells in the human whole blood, and the kit is simple and quick in operation, high in extraction efficiency, safe to use, and low in manufacture cost. By applying the kit, the DNA in white cells of a clinical whole blood sample for PCR (Polymerase Chain Reaction) technology detection can be rapidly extracted, and DNA in white cells and DNA of viruses in the white cells in whole blood are extracted.

A method for synthesizing Salacinol, its stereoisomers, and analogues, homologues and other derivatives thereof potentially useful as glycolsidase inhibitors. The compounds of the invention may have the general formula (I) or (II):

The synthetic schemes comprise reacting a cyclic sulfate with a 5-membered ring sugar containing a heteroatom (X). The heteroatom preferably comprises sulfur, selenium, or nitrogen. The cyclic sulfate and ring sugar reagents may be readily prepared from carbohydrate precursors, such as D-glucose, L-glucose, D-xylose and L-xylose. The target compounds are prepared by opening of the cyclic sulfates by nucleophilic attack of the heteroatoms on the 5-membered ring sugars. The resulting heterocyclic compounds have a stable, inner salt structure comprising a heteroatom cation and a sulfate anion. The synthetic schemes yield various stereoisomers of the target compounds in moderate to good yields with limited side-reactions.

The invention relates to the biotechnology field and in particular relates to a comprehensively optimized technological method for amplifying recombinant adenovirus. The method is used for amplifying human embryonic kidney cells (HEK293) by using poly-fiber paper carriers through a bioreactor; in this way, the whole set of process flow of adenovirus replication and amplification is established. The optimized technological method provided by the invention comprises the following steps of: under the condition of comprehensively adapting to type 5 adenovirus replication and amplification system, utilizing a DMEM culture medium containing 10% of blood serum at the cell culture stage, utilizing a DMEM culture medium containing 20% of blood serum in 20 hours after virus inoculation absorption; employing a blood serum-free culture medium added with 0.25% of lactoalbumin hydrolysate at the virus collecting stage, and simultaneously, supplementing 2g/L glucose every 20 hours. The way of batch collection and batch liquid exchange is adopted; and the method enables the optimized process to achieve high virus titer on the basis of reducing the later-stage purification difficulty and meeting the requirements of biological products. Therefore, the optimized process established by the invention an efficient recombinant adenovirus amplification process having excellent repeatability, and is suitable for any bioreactor with the poly-fiber paper as the carriers to amplify the recombinant adenovirus.

The invention discloses a microbial complex microbial agent for degrading lactic acid, and belongs to the technical field of microbes. The degradability of the pichia kudriavzevii provided by the invention to the lactic acid is up to 12.69 g/L, and is 2.04 times that of a model strain; at the same time, the strain can metabolize ethanol, and the OD600 can reach 4.48 after the strain is fermented in a sorghum juice culture medium at 30 DEG C and 200 rpm for 3 days; after fermentation is performed for 60 h, 58 g/L glucose in the sorghum juice culture medium can be completely depleted, and 13.06g/L ethanol can be produced; and the pichia kudriavzevii can relieve lactic acid pressure in a fermentation system when degrading the lactic acid, so that saccharomyces cerevisiae can grow and be metabolized to produce liquor.

The invention discloses a banana fusarium oxysporum culture medium and application thereof. The culture medium comprises the following components: 10g/L glucose, 2-8g/L aspartic acid, 5% by volume of20*nitrate, 0.5% by volume of 200*ferric salt, 0.1% by volume of 1000*vitamins and 0.1% by volume of 1000*trace elements, and the pH value of the culture medium is 6.2-6.5. By adopting the culture medium disclosed by the invention, germination of banana fusarium oxysporum conidium is promoted, interference of macromolecule proteins is avoided, and analysis on banana fusarium oxysporum secretion proteins is facilitated.

The invention discloses a semicontinuous fermentation method for producing gamma-aminobutyric acid by lactic acid bacteria, which comprises the following steps: inoculating lactic acid bacteria onto an Man, Rogosa and Sharpe gelatin (MRSG) culture medium; and fermenting in a mode of segmental substrate addition, segmental temperature control and segmental oxygen control to obtain gamma-aminobutyric acid, wherein the solvent of the MRSG culture medium is water, and the solute comprises the following materials at concentration: 7 to 12g/L glucose, 20 to 30g/L peptone, 30 to 40g/L beef extract, 0.05 to 1g/L manganese sulfate, 0.01 to 0.05g/L vitamin B, and 2 to 5g/L sodium glutamate. When the method provided by the invention is adopted, the yield of the gamma-aminobutyric acid can reach 13.1to 38.7g/L.

The application discloses a debonding protectant. The debonding protectant is prepared through mixing the following ingredients: dispase II with a concentration of 14g/L to 58g/L, kanamycin with a concentration of 14g/L to 58g/L, collagenase with the content of 0.8*10<6>U/L to 1.25*10<6>U/L and 0.7g/L-2.2g/L glucose. Simultaneously, the application provides a method for preparing a monocell suspension with high survival rate. Specifically, tissue blocks can be dispersed into single cells in ultrashort time through ablation action of an ultrasonic ablatograph, and cytoactivity is reserved as much as possible, and destruction to the cells is not caused. According to the application, the technical problem in applications of primary cell culture and monocell sequencing that high-activity primary cells are required to be acquired is excellently solved. Meanwhile, consumed time is greatly shortened, the whole dispersion process is shortened into several seconds from several hours required bythe existing enzyme digestion method, the stability is high, the mono-cellularization is good, and the activity of the cells is high.

The invention discloses a control method for efficient production of calcium gluconate by microbial fermentation. The method includes: taking a gluconic acid high-producing strain as the starting strain, conducting seed culture, then transferring the strain into a fermentation tank to perform fermentation production of calcium gluconate, and during fermentation, according to an online measured oxygen transfer coefficient (Kla), automatically controlling the adding speed and adding amount of 500g/L glucose and nutrients. The method realizes direct, real-time and accurate control of a calcium gluconate fermentation process.

Disclosed is a high-yield method for using a multi-step fermentation method to manufacture glycerin, which belongs to the technical field of biochemical industry. Through adjusting the ventilation capacity and the rotating speed, the fermentation can be divided into four stages for staged-fermentation with the process steps: a hyperosmotic yeast starter liquid which accounts for 5 to 10 percent of the inoculation amount according to the volume is added into an initial fermentation medium containing 250 g/l to 380 g/l glucose concentration, and the aerobic fermentation is performed under the condition of 30 DEG C to 40 DEG C; the ventilation capacity is turned up every 6 hours to finally reach 0.6 vvm to 3.5 vvm, the stirring rate reaches 70 rpm to 600 rpm, when the glucose concentration is lower than 30 g/l to 50 g/l, the multi-step fermentation enters the third stage of fermentation, when the ventilation capacity is turned down every 4 hours to 6 hours to finally reach 0 vvm, the stirring rate reaches 10 rpm to 50 rpm, the multi-step fermentation enters the forth stage of anaerobic fermentation, and when the glucose concentration is lower than 2 g/l, the fermentation stops. After the fermentation is finished, the fermentation liquor undergoes solid-liquid separation, and the glycerin is prepared into products through separation and purification. The invention has the advantages that the residual sugar is obviously reduced at the later stage of fermentation, and the glycerin is not re-consumed.

The invention discloses a method for preparing lactobacillus acidophilus powder by freeze-drying in vacuum. The method comprises the following steps of: adding lactobacillus acidophilus into a culture medium for culturing the lactobacillus acidophilus; after centrifugal collection of thalli, washing the thalli with physiological saline and centrifuging to remove clear liquid; after preparing the thalli into bacterial suspension by using a protecting agent I; adding a protecting agent II; and finally performing freeze-drying, wherein the formula of the protecting agent I comprises the following components: 10 to 20g/L trehalose, 10 to 20 g/L glycerol, 5 to 15 g/L bovine serum albumin, 6.5 to 8.5g/L sodium chloride and 60 to 100 g/L sucrose; and the formula of the protecting agent II comprises the following components: 150 to 250g/L skim milk powder, 10 to 40g/L glucose, 6.5 to 8.5g/L sodium chloride, and 0.2 to 0.3g/L ascorbic acid. The survival rate of the lactobacillus acidophilus powder prepared by the invention is up to 99.9 percent.

A method for detecting bioavailable lead in soil comprises the following steps: s100, collecting and pretreating a sample, to be more specific, collecting the soil, air-drying the collected soil at room temperature, grinding, sieving by a 200-mesh sieve to prepare the soil sample, weighing 1-5 g of the soil sample, and putting the soil sample into a 250 ml polytetrafluoroethylene centrifugal cup,respectively adding 25 ml of a 1 mol/L strontium chloride solution and 25 ml of a 0.5mol/L glucose solution, stirring by a constant-temperature magnetic stirrer, centrifuging by a centrifugal machine,taking supernate, filtering the supernate with a filtering membrane to obtain an extracting solution, and diluting the extracting solution to a scale by using a HNO3 solution with the concentration of 1%; s200, determining the concentration of the bioavailable lead in the extracting solution, to be more specific, establishing a standard curve used for determining the different concentrations of the bioavailable lead by adopting an atomic absorption spectrometer through a flame atomic absorption method, and measuring the concentration of the bioavailable lead in the extracting solution throughthe standard curve by adopting the atomic absorption spectrometer; and s300, analyzing results, to be more specific, according to the measured mass of the soil sample and the measured concentration of the extracting solution, calculating the content of the bioavailable lead in the measured soil sample.

The invention relates to a method for preparing sulforaphane by using glucoerucin, comprising the following steps: (1) preparing glucoerucin; (2) preparing L-glucose sulforaphane: adding sulforaphane obtained by the step (1), (+)-L-diethyl tartrate, alkoxy titanium or alkoxy vanadium compound in a mixed solution of methanol, ethanol and ethyl acetate to obtain a product of L-glucose sulforaphane;(3) taking broccoli seeds and crushing, filtering through gauze to obtain a filtrate, adding ammonium sulfate in the filtrate at a temperature of 4 DEG C with a saturation of 55 %, mixing uniformly, centrifuging the precipitate to dissolve the precipitate in deionized water, and dialyzing overnight to obtain a crude enzyme of hydrolase; and (4) purifying the L-glucose sulforaphane. The method aims at overcoming the disadvantages of high cost of raw materials, low yield of sulforaphane and the like. According to the invention, sesame seeds which are cheaply obtained are used as raw materials, thus the cost of the raw materials is one fifth that of broccoli seeds, and the sulforaphane yield obtained by using sesame seeds is 3 times the yield obtained by using broccoli seeds.

The invention discloses construction and application of heat-resistant yeast engineering bacteria capable of producing glycerine under high temperature aerobiotic condition. A heat-resistant kluyveromyces marxianus is used as a platform, methods of genetic engineering, metabolic engineering and the like are utilized, a strain having triose phosphate isomerase (TPI1) deletion is constructed, and the constructed strain has the capacity of efficiently producing the glycerine through efficient utilization of glucose, levulose and xylose under the high temperature aerobiotic condition. The obtainedkluyveromyces marxianus strain YZB115 produces 40.32 g/L glycerine through 80g/L glucose, produces 41.84 g/L glycerine through 80g/L levulose and 18.64g/L glycerine through 80g/L xylose under the aerobiotic condition of 42 DEG C, the production rates are respectively 0.84g/L/h, 0.50g/L/h and 0.22g/L/h, the yields are respectively 0.50g/g, 0.50g/g and 0.23g/g, in the fermentation process of the constructed engineering strain, a by-product namely ethanol is not produced, and xylitol is not accumulated when the operation of producing the glycerine through fermentation of xylose is ended.

The invention discloses a formula and preparation method of high-purity medicinal nano silver paste. According to the formula and preparation method of the high-purity medicinal nano silver paste, specific high-purity spherical ultrafine nano silver powder and the medicinal nano silver paste are selected. According to the indexes of the specific high-purity spherical ultrafine nano silver powder, the silver purity is larger than or equal to 99.99 percent, the silver shape is spherical, and the silver particle size ranges from 0.1 nm to 10 nm. According to the formula of the medicinal nano silver paste, the medicinal nano silver paste comprises 1 g/L to 2 g/L glucose, 1 g/L to 2 g/L nano-silver, 996 g/L to 998 g/L pure water. The preparation method of the medicinal nano silver paste comprises dispersing, stirring, atomizing, collecting and liquid taking.

The invention relates to a Klebsiella sp. and its application. The Klebsiella sp. DHU-S1 has the preservation number of CGMCCC No. 18075, and its nucleotide sequence is as shown in SEQ ID NO. 1. The Klebsiella sp. is used for dissolving and killing blue-green algae in a water body. The most suitable algae-dissolving conditions of the strain are as follows: inoculation amount is 10%, pH is 7.0, temperature is 25 DEG C, and 10g/L glucose is added as a supplementary carbon source. Under the conditions, the dissolution rate of blue-green algae by the Klebsiella sp. reaches 100%.