13 results about "L-Glucose" patented technology

The invention relates to a culture medium for serum-free suspension culture of F81 cells and application of the culture medium, the culture medium comprises the following components in concentration: 4000-4500 mg / L of amino acid, 80-120 mg / L of vitamin, 5000-5100 mg / L of inorganic salt, 5000-5500 mg / L of glucose, 1000-1030 mg / L of adherence promoting substance, 20-40 mg / L of growth factor, 2000-2040 mg / L of buffering agent and 2-2.4 mg / L of antioxidant, the pH value of the culture medium is 7.0-7.2, and the pH value of the culture medium is 0.5-1.5. The antioxidant is prepared from partially hydroxylated glutathione and a carboxymethyl chitosan-gallic acid grafted copolymer. The partially hydroxylated glutathione and the carboxymethyl chitosan-gallic acid graft copolymer are compounded to serve as an antioxidant of the culture medium, so that the oxidation resistance of the culture medium is enhanced synergistically, and the cell survival rate is increased.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The invention discloses a low-temperature preservation method of Octopus variabilis sperms and spermatophore, the Octopus variabilis sperms adopt an ultralow-temperature cryopreservation method, the Octopus variabilis sperms are protected by using a cryoprotectant composed of a 0.6 mol / L glucose solution diluent and 20% glycerol, the diluent contains nutrient substances, and the Octopus variabilis sperms have the characteristics of inactivation of sperms and small damage to sperms. The cryoprotectant has the functions of a permeable antifreeze agent and a non-permeable antifreeze agent, so that the sperms are subjected to dual protection in the freezing process; the octopus variabilis spermatophore is preserved at a low temperature, a 1.0 mol / L glucose solution is used as a spermatophore protection solution, the spermatophore structure can be maintained within one month, and high sperm activity of octopus variabilis sperms can be maintained within a short period (one month). Based on requirements in experiments, two octopus variabilis sperm preservation methods are provided and can be flexibly used, and technical support is provided for preservation of octopus variabilis germplasm resources and overcoming of the problem of asynchronous male and female development.

The invention provides a strain for producing mannitol and a method for producing mannitol by using the strain, and belongs to the technical field of food biology. According to the present invention, the fermentation lactobacillus fermentum SK50.004 is screened from the pickled vegetables, and the strain is used for the fermentation production of the mannitol; by optimizing the carbon source composition and inorganic salt components in the fermentation medium, the yield of mannitol produced by fermentation of the strain at 37-40 DEG C under the conditions that the concentration of CuSO45H2O is 10 mg / L and the total sugar concentration is 265 g / L (glucose is 80 g / L and fructose is 185 g / L) reaches 160 g / L, the maximum productivity is 4.21 g / L / h, byproducts in the fermentation process are reduced, the production efficiency is remarkably improved, and the production cost is reduced. And a reliable technical support is provided for industrial efficient production of mannitol.

The application discloses a recombinant domain fusion enzyme and application thereof in preparation of D-allulose, wherein the recombinant domain fusion enzyme is formed by splicing and recombining a 5' domain and a partial 3' domain of D-tagatose 3-epimerase and an active center domain of xylose isomerase. The application uses the domain fusion enzyme of the 5' domain and the partial 3' domain of D-tagatose 3-epimerase and the active center domain of xylose isomerase as a biological catalyst, and the yield of D-allulose generated by catalyzing isomerization of 260 g / L glucose is 35.9%. The method disclosed by the application is rapid and efficient, reaction conditions are simple, substrate conversion rate is high, and byproducts are few, so that the pressure of separation and purification is reduced, and the method is suitable for large-scale industrial production.

The invention discloses saccharomyces cerevisiae capable of efficiently utilizing cellulose hydrolysate as well as a construction method and application thereof. The construction method comprises the following steps: 1) selecting a xylose metabolism saccharomyces cerevisiae haploid chassis strain of a xylose isomerase pathway; 2) performing spore splitting on industrial ethanol-producing yeast to obtain haploid industrial yeast strains; and (3) fusing and screening the strains obtained in the steps (1) and (2) to obtain the saccharomyces cerevisiae capable of efficiently utilizing the cellulose hydrolysate. The saccharomyces cerevisiae. At the shake flask level, 70 g / L of glucose in the acid-treated cellulose hydrolysate can be completely consumed within about 24 h, meanwhile, xylose starts to be consumed gradually, and 20 g / L of xylose in the acid-treated cellulose hydrolysate can be consumed within 72 h. The growth amount OD600 can reach about 15.0 at most, 45 g / L of ethanol is generated under the aerobic condition, rapid growth in industrial cellulose hydrolysate is achieved, glucose and xylose are fully utilized as growth carbon sources, and ethanol is generated.

This invention discloses a high-antigenic-activity avian Pasteurella multocida culture medium, its preparation method, and its application. The high-antigenic-activity avian Pasteurella multocida culture medium consists of: 8-13 g / L bacteriological peptone, 3-8 g / L growth-promoting peptides, 18-23 g / L yeast extract, 2-7 g / L glucose, 4-9 g / L sodium chloride, 1-5 g / L dipotassium hydrogen phosphate, and the remainder being water. Using this high-antigenic-activity avian Pasteurella multocida vaccine culture medium promotes bacterial growth and reproduction, requires a shorter culture time, and achieves a high cell density, ultimately reaching 1.84 × 10¹⁰ CFU / mL. It significantly improves fermentation speed and the cell density of avian Pasteurella multocida in the fermentation broth, making it more suitable for culturing avian Pasteurella multocida. Furthermore, the vaccine prepared using the culture medium provided by this invention has a protection efficiency of up to 100% and excellent antigenic activity, laying the foundation for the production of avian Pasteurella multocida vaccines.

The application discloses a kind of recombinant escherichia coli and its construction method and the method for producing glucaric acid by metabolic engineering, belong to the field of metabolic engineering.The application realizes the production pathway of glucuronic acid by expressing the myo-inositol-1-phosphate synthase gene (Ino1) and myo-inositol oxidase gene (MIOX) derived from plants in escherichia coli, simultaneously expresses the aldehyde acid dehydrogenase gene (Udh) derived from Agrobacterium tumefaciens, and successfully constructs the synthesis pathway of glucaric acid.The recombinant escherichia coli generates glucaric acid 2.53g / L in LB-G (10g / L glucose is added in LB medium) fermentation medium.The method for fermenting and producing glucaric acid by the recombinant escherichia coli proposed in the application has broad development prospect, and lays the foundation for biosynthesis of glucaric acid.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

The invention provides a self-induction culture medium suitable for multi-plasmid co-expression recombinant bacteria and application of the self-induction culture medium. The self-induction culture medium is prepared from the following components: 0.6 to 1 g / L of glucose, 3 to 5 g / L of lactose, 5 to 15 g / L of glycerol, 5 to 15 g / L of yeast extract, 6.8 to 13.6 g / L of Na2HPO4, 3.0 to 6.0 g / L of KH2PO4, 0.5 to 10 g / L of NaCl, 1.0 to 3.0 g / L of NH4Cl, MgSO4 with the final concentration of 1 to 3 mol / L, CaCl2 with the final concentration of 0.1 to 2 mol / L, 0.15 to 10 g / L of methionine and 1 to 2 mg / L of vitamin B12. The self-induction culture medium is simple in component and convenient to operate, recombinant escherichia coli E.coli BL21 (DE3)-VA which simultaneously carries three recombinant expression vectors pETDuet-1, pCDFDuet-1 and pRSFDuet-1 and can express carboxylic acid reductase, methyltransferase and 3-dehydroshikimic acid dehydratase can be induced and cultured, the self-induction culture medium is used for fermentation production of vanillyl alcohol, the yield of vanillyl alcohol can reach 277.85 mg / L after fermentation is performed for 48 h, and the yield is 3.8 times that of an IPTG induction mode.

The invention discloses a recombinant structural domain fusion enzyme and application thereof in preparation of D-psicose. The recombinant structural domain fusion enzyme is formed by splicing and recombining a 5'structural domain and a part of 3 'structural domain of D-tagatose 3-differential structure enzyme with an active center structural domain of xylose isomerase. According to the invention, a structural domain fusion enzyme of a 5'structural domain and a part of 3 'structural domain of D-tagatose 3-epimerase and an active center structural domain of xylose isomerase is used as a biocatalyst, and the yield of D-psicose generated by catalyzing 260g / L glucose isomerization reaction is 35.9%. The method has the advantages of rapidness, high efficiency, simple reaction conditions, high substrate conversion rate, few by-products, reduction of the pressure of separation and purification, and suitableness for large-scale industrial production.

The invention discloses a Prussia adamsii BQS-1 (Bacillus amyloliquefaciens BQS-1) and an application thereof. Relates to the technical field of microorganisms. The strain provided by the invention can be used for degrading zearalenone in feed. In a zearalenone solution with the initial concentration of 2 mg / L, the degradation rate can reach 94.9 + / -0.5% after the strain acts for 1 d. The corn flour and the microbial inoculum are uniformly mixed, and the ZEN content is reduced by 61.8 + / -6.8% within 24 hours. If the pH value of the corn flour is adjusted to be neutral and 4 g / L glucose is added into a fermentation culture medium, the degradation effect can be further improved, and the degradation rate is increased to 75.8 + / -3.5%. Therefore, the prelisteria aryabhattai BQS-1 has a wide application prospect in the aspect of developing the ZEN degrading bacterial agent.

The invention discloses a ginseng adventitious root tissue culture medium and an induction method and application thereof. Relates to the technical field of tissue culture. Comprising the following raw materials: MS (Murashige and Skoog) used as a basic culture medium, 20 to 30 g / L of cane sugar, 5 to 10 g / L of glucose, 2 to 3 mg / L of IBA (Indole Butyl Acetate), 0.2 to 0.3 mg / L of NAA (Naphthalene Acetic Acid), 0.05 to 0.1 mg / L of The invention further provides an induction method and application. Compared with an earlier-stage research result, the total saponin content is further increased, and the adventitious root induction rate is increased to a certain extent.