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Klebsiella sp. and application thereof

A technology of Klebsiella, cgmccno.18075, applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problems that cannot be well satisfied with fast, effective, and cheap removal of harmful algae, and achieve thorough The effect of killing algae cells

Active Publication Date: 2019-11-22
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional chemical, physical, and biological treatment measures cannot well meet the requirements of fast, effective, and cheap removal of harmful algae in water bodies

Method used

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  • Klebsiella sp. and application thereof
  • Klebsiella sp. and application thereof
  • Klebsiella sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Strain isolation

[0042] (1) Collect water samples from Jingyue Lake, where algal blooms bloom in summer on the Songjiang Campus of Donghua University. Collect water samples and save them in sterile Erlenmeyer flasks, place the Erlenmeyer flasks in an incubator filled with ice cubes, and immediately transfer them to a sterile laboratory for colony enrichment culture.

[0043] (2) The above-mentioned collected water samples were passed through 10 -1 ,10 -2 ,10 -3 ,10 -4 ,10 -5 Five gradient dilutions were made. After dilution, the solutions were respectively spread on the aseptic plate medium of Microcystis aeruginosa under aseptic conditions, and placed in a 37°C incubator for enrichment culture. After 24 hours of culture, a single colony was obtained.

[0044] (3) Observe the shape of the grown colony, pick a single colony with an inoculation loop, and separate it by streaking on a sterilized LB solid medium plate. Repeat the separation and purification for 5...

Embodiment 2

[0053] Algae-dissolving test of Klebsiella sp. DHU-S1 on Microcystis aeruginosa

[0054] The specific implementation is as follows:

[0055] 1. Inoculate the strain DHU-S1 obtained above into 100mL inorganic salt liquid medium, and cultivate to the logarithmic phase;

[0056] 2. Culture Microcystis aeruginosa (Microcystis aeruginosa) FACHB905 in sterile BG11 medium and cultivate to the logarithmic phase; Microcystis aeruginosa culture conditions: in a constant temperature light incubator at 25°C, light: dark cycle ratio = 12h : 12h.

[0057] 3. Take the above Klebsiella sp. (Klebsiella sp.) DHU-S1 bacterium liquid cultured to the logarithmic phase, add it to the algae liquid of Microcystis aeruginosa cultured to the logarithmic phase at a volume fraction of 10%, and place it in 25 ℃ light incubator, with light: dark cycle ratio 12h: 12h co-cultivation. Another microcystis aeruginosa liquid added with the same volume fraction of inorganic salt medium (MSM) was taken as the c...

Embodiment 3

[0061] Effect of initial algae concentration on algicidal efficiency of Klebsiella sp. DHU-S1.

[0062] The specific implementation is as follows:

[0063] 1. Inoculate Klebsiella DHU-S1 in 100mL inorganic salt liquid medium and cultivate to the logarithmic phase;

[0064] 2. Add different amounts of Microcystis aeruginosa cultured to the logarithmic phase into sterile BG11 medium to obtain four groups of Microcystis aeruginosa liquids with different algae cell concentrations, each with three replicates. Experimental group 1 concentration: 1×10 5 CFU / mL. Experimental group 2 concentration: 1×10 6 CFU / mL. Experimental group 3 concentration: 1×10 7 CFU / mL. Experimental group 4: 1×10 8 CFU / mL.

[0065] 3. Take the bacterium liquid of Klebsiella sp. DHU-S1 cultivated to the logarithmic phase, and add it to the aeruginosa of experimental group 1, experimental group 2, experimental group 3, and experimental group 4 with a volume fraction of 10%. Microcystis algal liquid. A...

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Abstract

The invention relates to a Klebsiella sp. and its application. The Klebsiella sp. DHU-S1 has the preservation number of CGMCCC No. 18075, and its nucleotide sequence is as shown in SEQ ID NO. 1. The Klebsiella sp. is used for dissolving and killing blue-green algae in a water body. The most suitable algae-dissolving conditions of the strain are as follows: inoculation amount is 10%, pH is 7.0, temperature is 25 DEG C, and 10g / L glucose is added as a supplementary carbon source. Under the conditions, the dissolution rate of blue-green algae by the Klebsiella sp. reaches 100%.

Description

technical field [0001] The invention belongs to the field of water body purification and treatment, and in particular relates to Klebsiella and its application. Background technique [0002] In recent years, due to the increasing use of chemical products in industry and agriculture, the discharge of production and domestic wastewater into natural water bodies such as rivers and lakes has resulted in excessive nitrogen and phosphorus nutrient elements in the water body, causing severe water environmental problems. Typical such as water eutrophication. Algae multiply in eutrophic water bodies, and most harmful algae can secrete algal toxins. Algal toxins are highly toxic substances that are not easy to degrade under natural conditions, and will eventually accumulate to a lethal dose. Algae overgrowth in water also competes with other aquatic organisms for free oxygen. Eventually lead to the death of aquatic organisms, after the death of organisms, the organisms decompose and...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/22
CPCC12N1/20C02F3/34C12R2001/22C12N1/205
Inventor 赵晓祥李东鹏孙铸宇亢昕康雅欣刘靖宇
Owner DONGHUA UNIV
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