This invention discloses a
Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General
Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of
Bacillus subtilis strain EB-8 in the
fermentation modification of tartary buckwheat flour is also disclosed. This invention's
Bacillus subtilis strain EB-8 can significantly increase the
amylose content of tartary buckwheat, helping to inhibit
starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total
flavonoid and total phenolic content of tartary buckwheat, enhancing its
antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as
lysine and
methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold
processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the
functional activity and
processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.