The invention relates to the technical field of
plant tissue culture and genetic transformation, and particularly discloses a stable genetic transformation method of lily, which comprises the following steps: infecting a transformation donor by taking a scale of a lily seedball as a transformation
receptor, co-culturing after infection, and
cutting the co-cultured scale to a detoxification culture medium containing
cephalosporin for detoxification to obtain the stable genetic transformation of the lily. Then inoculating to a first screening culture medium containing
kanamycin and
cephalosporin for screening culture to obtain a resistant
seedling, inoculating the resistant
seedling to a second screening culture medium containing
kanamycin for screening culture, and planting the resistant
seedling in a culture medium for culture to obtain a resistant
plant. According to the method, the scales of the lily bulbs are directly used as transformation receptors,
agrobacterium tumefaciens infection is performed, detoxification and screening culture are performed respectively to obtain stable genetic transformation resistant plants of the Lanzhou lily, sterile seedling culture is not needed, the transformation receptors are sufficient and easy to obtain, the operation process is simple and convenient, no
pollution is caused, transgenic identification is easy, and false positive is low; the whole genetic transformation period is shortened.