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Method for remedying polluted soil through multi-pond-alga water circulation irrigation system

ActiveCN107971334ASolve the problem of repairing high water consumptionImprove fertilityContaminated soil reclamationSoil-working methodsAutomatic controlAdditive ingredient
The invention provides a method for remedying soil heavy metal pollution, improving saline and alkaline land and recycling microalgae for resource utilization through a multi-pond-alga water circulation irrigation system, and belongs to the field of soil remediation and resource utilization. Through the interaction of man-made ponds, microalgae and soil, a pond-microalga combined remediation system is adopted as the core, the soil is prepared and domesticated to remedy the microalgae, the multiple ponds are used for collecting rainwater, the microalgae are cultivated and subjected to expandingpropagation, and the microalgae are sprayed to the field; through circulation irrigation, multi-time spraying and alga water recycling, heavy metal in the polluted soil is taken away, the salinity ofthe soil is reduced, the nutritional ingredients of the soil are improved, and the soil fertility is restored; the microalgae are harvested through the method of pond enrichment and flocculation andaeration, the alga charcoal preparation process is utilized through combination, and resource utilization is achieved; and the system achieves automatic control through a sensor, a PC and a control component. The remediation method comprises a heavy metal/saline and alkaline land remediation microalga cultivation system, a water collection system, an intelligent spray irrigation unit, a microalgarecycling system and a microalga resource utilization system.
Owner:江苏氿官资源环境研究院有限公司

Pseudomonas aeruginosa nucleic acid fluorescent PCR (polymerase chain reaction) detection kit and detection method

The invention discloses a Pseudomonas aeruginosa nucleic acid fluorescent PCR (polymerase chain reaction) detection kit and detection method. The detection kit comprises a PCR reaction solution, an enzyme mixed solution, a positive control, a negative control and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, primers for target polynucleotide amplification and probes for target polynucleotide detection; the primers comprise a forward primer and a reverse primer. Or, the detection kit comprises a PCR reaction solution, an enzyme mixed solution and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, a forward primer for target polynucleotide amplification, a reverse primer for target polynucleotide amplification and probes for target polynucleotide detection. The Pseudomonas aeruginosa nucleic acid fluorescent PCR detection kit shown in the embodiment of the invention with the advantages of no need of nucleic acid extraction, high detection sensitivity, wide detection range and high detection accuracy can quickly and accurately detect Pseudomonas aeruginosa DNA (deoxyribonucleic acid) in plasma, urine and other samples.
Owner:SANSURE BIOTECH INC

Imaging model in small animal living bodies with echinococcus granulosus and construction method thereof

The invention discloses an imaging model in small animal living bodies with echinococcus granulosus and a construction method thereof. The construction method comprises the steps of: feeding CF-1 male mice; carrying out in vitro culture and drug treatment on protoscolex; carrying out in vitro fluorescence imaging and fluorescence gradient analysis; pretreating mice with depilatory paste for experimental animals, injecting the abdominal cavity of each mouse with chloral hydrate anesthetic, implanting three groups of stained scoleces under Glisson capsules by injection, dividing the mice into three groups according to different groups of injected protoscolex; in a control group, injecting the livers of mice with protoscolex on which drug treatment is not carried out, collecting images every 12 hours after injection, carrying out fluorescence intensity analysis on the ROIs region, putting into a polyethylene cage after imaging, and carrying out in vitro fluorescence gradient detection in the positive control group. The construction method constructs a mouse model with echinococcus granulosus which can express luciferase, provides a platform for drug sensitive tests in living bodies with echinococcosis, and can monitor the growth and transfer of hydatid in the living model dynamically in a long term.
Owner:王思博
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