199 results about "Microcystis aeruginosa" patented technology

The invention relates to a preparation and the preparation method for restoring the microecological bacteria on the interface layer of water-sediment. The microecological bacteria preparation uses 0.2-0.4mm of medical stone as carrier, absorbs and fixes the composite microbe optimally combined by bacillus subtilis, bacillus licheniformis, bacillus megatherium, and thin yellow streptomyces. The amount ratio of bacillus subtilis to bacillus licheniformis to bacillus megatherium approximates to one to one to one; the volume ratio of the mixture liquid of the three kinds of bacillus to the thin yellow streptomyces liquid is two to one. The invention has the advantages that spreading the preparation of the invention into the interface layer of functional water body water-sediment in the spring March to April each year can prevent blue algae (aerugo micro-capsule algae is the most) from fulminantly growing in summer June to August. The preparation can improve the quality of water, can prevent eutrophication development, and can promote the carbon, nitrogen, and oxygen circulation on the interface layer of water-sediment. The microecological bacteria preparation has high effective colony amount and strong adaptability; and is effective to restore the microecological environment at the interface layer of water-sediment.

The invention discloses a method for determining the biotoxicity of atrazine by utilizing microcystis aeruginosa. The method comprises the following steps: A, cultivating the microcystis aeruginosa; B, preparing microcystis aeruginosa liquid; C, determining the response time of the chlorophyll fluorescence of the microcystis aeruginosa on the biotoxicity of the atrazine; and D, drawing the standard curve for determining the biotoxicity of the atrazine by utilizing the chlorophyll fluorescence of the microcystis aeruginosa, namely adding atrazine standard liquid with series concentration into the preparation liquid of the microcystis aeruginosa, completely mixing until the 'optimum response time' determined by the step C and determining the chlorophyll fluorescence intensity of the microcystis aeruginosa; and setting three parallel samples for each concentration, setting a blank sample by substituting aseptic distilled water for the sample, calculating the photosynthetic inhibition ratio, and finally drawing the 'dose-effect relation curve of atrazine concentration and photosynthesis inhibition ratio' to serve as the standard curve for quantitatively determining the biotoxicity of the atrazine by the chlorophyll fluorescence method of the microcystis aeruginosa. The method can determine the biotoxicity of the atrazine simply, conveniently and quickly, and is low in cost and environment-friendly.

The present invention belongs to the technical field of environment protection, in particular to a method to degrade Microcystic aeruginosa through microorganisms. The method is that firstly an algae-lysing bacterial strain Brevibacillus spp.FDK2 can be achieved by separating from and sublimating deep eutrophication urban lake water body;the algae-lysing bacterial strain Brevibacillus spp.FDK2 is cultured by a culture medium to acquire bacterial liquid with a certain concentration. The bacterial liquid is added into a water sample containing Microcystic aeruginosa. Under a certain condition, the bacterial strain has a good degrading effect on the Microcystic aeruginosa. The degrading removing rate is more than 90 percent.

The invention relates to a microcystic aeruginosa Chinese herbal medicine algal inhibiter and a preparation method. The invention belongs to the water pollution control technical field. The microcystic aeruginosa Chinese herbal medicine algal inhibiter is a Chinese herbal medicine-based algal inhibiter and is characterized in that the Chinese herbal medicine coptis chinensis is boiled to obtain the algal inhibiter. The preparation method of the microcystic aeruginosa Chinese herbal medicine algal inhibiter is liquid preparation and is characterized in that the Chinese herbal medicine coptis chinensis is put into water and boiled to obtain the Chinese herbal medicine liquid preparation. The product has the advantages of excellent performance, high efficiency in inhibiting microcystic aeruginosa, rapid effect, easy use, wide application range, simple preparation method, easy operation, high efficiency, low toxicity and low preparation cost, which can be widely used in artificial water supply systems, reservoirs, ponds and lakes and other water bodies.

The present invention discloses a method for culturing golden algea and an application thereof in controlling blooming algae, which belong to the field of water pollution control technology. The method for culturing golden algea is as follows: golden algea and microcystic aeruginosa are added into the BG11 culture medium; the golden algea lives on the microcystic aeruginosa to grow heterotrophically; after seven to ten days of culturing, the microcystic aeruginosa is gradually eaten up; the golden algea gets into the stable growing period; high-density golden algea liquid is obtained; and the golden algea liquid is centrifugated in order to harvest the golden algea. The golden algea harvested by centrifugating or the golden algea liquid is directly added into the water body, and the golden algea swallows the blooming algae, so that the overgrowth of the blooming algae can be controlled and the blooming algae can be eliminated. The method which utilizes the golden algea to swallow the blooming algae to prevent the outbreak of the blooming algae and control the growth of the blooming algae is an algae-inhibiting method which is characterized by good ecological safety, cheap materials, economy, high efficiency and easy utilization, and has the advantages of easy golden algea culturing, good algae control effect, etc.

The invention discloses a method for separating and purifying microcystin by utilizing series-connected solid phase extraction columns. The method comprises the following steps of: A, preparing an algae powder material, namely harvesting wild bloom-forming cyanobacteria or microcystis aeruginosa cultured indoors, cooling and drying to prepare dried algae powder; B, preparing the solid phase extraction columns, namely putting C18 fillers into Solid Phase Extraction (SPE) columns; C, extracting algae toxin, namely weighing the dried algae powder, adding methanol according to a proportion, putting on a shaking table and oscillating at room temperature; D, removing impurities, namely adding a supernatant and an organic extractant into a separating funnel according to a proportion, mixing and uniformly shaking, standing, and after delamination, removing the organic extractant; E, separating and purifying the algae toxin, namely after pretreatment, enriching sample solution by using pre-activated series-connected SPE columns, and eluting the toxin by using 20 to 50 percent methanol aqueous solution; and F, performing Methyl Cellulose (MC) detection. The method has good separation effect, large loading amount, is easy and convenient to operate and low in cost, can obtain two microcystins with the weight of more than or equal to 10 mg and higher purity each time, and the High Performance Liquid Chromatography-Ultra Violet/Diode Array Detector (HPLC-UV/DAD) detection can reach 85 to 90 percent.

The invention discloses a quick counting method for microcystis aeruginosa. The quick counting method comprises the following steps of: measuring the absorbancy of microcystis aeruginosa with different concentrations at wavelength of 680nm through a microplate reader, accurately counting frustules through a microscope at the same time, creating a good linear relation between the absorbancy and cell density, and quickly counting the microcystis aeruginosa in a sample to be measured through an obtained correction curve or regression equation. The simple, safe, effective and quick quantifying method for microcystis aeruginosa provided by the invention overcomes the defects of lack of algae monitoring and counting measures and low monitoring and counting efficiency in China.

The invention discloses a chromone-containing benzoyl hydrazone compound capable of suppressing growth of cyanobacteria in a general formula I, synthesis of the compound and an in-vivo experiment on the benzoyl hydrazone for suppressing the growth of the cyanobacteria. In the formula, an R<1> represents hydrogen, halogen, C1-C4 alkyl groups or hydroxyl groups, an R<2>, an R<3> and an R<4> respectively represent hydrogen, halogen, hydroxyl groups or trifluoromethyl, and substituent groups represented by the R<2>, the R<3> and the R<4> can be identical or different. In the in-vivo experiment on the compound to microcystis aeruginosa FACHB912 which is the main type of cyanobacteria, a half maximal effective concentration value EC<50> of the compound ranges from 0.17muM to 33.37muM, and thus the compound has a remarkable effect for suppressing the growth of the cyanobacteria and can be used as an effective ingredient of an algicide.

The invention discloses bacillus cereus and application thereof. The bacillus cereus has a very strong algal inhibiting effect, and particularly has a very strong inhibiting effect on microcystis aeruginosa, and the algal inhibition rate after a seven-day process is more than 90%. The bacillus cereus NMSW16 is simple in culture and fermentation, can be cultured in a large scale, can generate fermentation liquor through fermenting, and can be used for governing water bloom and restoring water eutrophication.

The invention discloses an algae inhibitor for verdigris microcystis aeruginosa. The algae inhibitor is composed of n-nonanoic acid and linoleic acid in the volume ratio of 1:1, the applicable concentration range of the algae inhibitor in verdigris microcystis aeruginosa ranges from 0.04mL/L to 0.08mL/L. Compared with the prior art, two different kinds of fatty acids, namely the n-nonanoic acid and the linoleic acid, are selected to be combined, after inhibition effects of the combined fatty acids are evaluated by means of three kinds of different evaluation systems, the result indicates that the combination of the fatty acids has excellent synergic inhibition effects indeed. Further, the algae inhibitor is low in cost, environment-friendly and safe.

The invention relates to an inhibition method of microcystis aeruginosa. A cordate houttuynia extract is used for inhibition. The inhibitor raw material of the inhibition method is rich and easy to get and environment-friendly during the treatment process of cyanobacterial bloom, and does not cause secondary pollution on water.

The invention discloses a method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa. The method comprises the following steps: A, preparing the microcystis aeruginosa chlorophyll a standard solution; B, measuring the concentration of chlorophyll a in a stock solution, and calibrating the concentration by the national standard method; C, achieving fluorimetric determination of the stock solution and respectively measuring the fluorescence intensities of all solutions; D, building a microcystis aeruginosa standard fluorescence curve, and drawing a microcystis aeruginosa chlorophyll a fluorescence curve according to the concentration and the fluorescence intensity of the stock solution; and E, building a microcystis aeruginosa calibration fluorescence curve. According to the calibration curve between the fluorescence intensity of the microcystis aeruginosa solution and the concentration of the chlorophyll a, the concentration of the chlorophyll a can be real-timely and quickly obtained only by directly measuring the fluorescence intensity of water samples through a luminoscope.

The invention relates to a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment, belonging to the technical field of environment-friendly water treatment. The invention provides Lysinibacillus fusiformis of which the collection number is CGMCC NO.7520. The invention also provides a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment. The screened algicidal bacteria has a certain dissolution action on the dominant alga strain microcystis aeruginosa in blue algae bloom, and the algicidal bacteria TR3 can be obtained by separation. Under the optimum conditions, the removal rate of the algicidal bacteria TR3 for microcystis aeruginosa within 96 hours can reach 97.18%, so the algicidal bacteria TR3 has very ideal alga dissolution effect; and thus, the algicidal bacteria TR3 can better display the alga dissolution characteristic in a natural water body, and has wider application prospects in large-area water body blue algae bloom pollution treatment.

The invention provides a strain of stenotrophomonas sp., which is named as stenotrophomonas F6, and has a preservation number of CGMCC No.6547. Two active algicidal substances, namely cyclo(glycine-proline) and hydroquinone, are separated from the metabolism products of the stenotrophomonas F6, and then the substances are purified and identified. The half effective concentration of the cyclo(glycine-proline) on inhibiting microcystis aeruginosa 9110 is 9.5 mg/L, and the cyclo(glycine-proline) has no inhibiting effect on synechococcus BN60. The half effective concentrations of hydroquinone on inhibiting microcystis aeruginosa 9110 and synechococcus BN60 are 0.96 mg/L and 5.6 mg/L. The stenotrophomonas F6 and the active algicidal substances (cyclo(glycine-proline) and hydroquinone) can be applied to the development and production of novel algicide, and finally are applied to the control of cyanobacterial bloom in fresh water.

The invention relates to an application of artemisinin as algae inhibiting agents. The algae is microcystis aeruginosa or scendesmus obliquus. The artemisinin is extracted from artemisia annua of terrestrial plant, is a degradable natural product with good environmental compatibility and lower toxicity on higher animals and has a wide application range as the algae inhibiting agents.

The invention belongs to the technical field of microbial engineering, and particularly relates to a microcystis aeruginosa lysozyme and an application thereof; specifically, the strain is heat-resistant bacillus sporothermodurans, and the preservation name of the strain is heat-resistant bacillus sporothermodurans sporothermodurans Z-1; the invention also optimizes an applicable condition culturesystem of the strain, discusses the algae-lysing mechanism of the strain, researches the influence of parameters such as temperature, concentration of added bacterial liquid and pH value on the algae-lysing efficiency, and tests show that the strain has good dissolving and eliminating effects on microcystis aeruginosa, and mainly exerts the algae-lysing effect through extracellular products, wherein the efficiency of treating microcystis aeruginosa is highest under the conditions that the temperature is 25-40 DEG C, the illumination intensity is 1800-2200 lux, the light and dark period is (10-15) h: (10-15) h, and the pH value is 6-9, and the method can be applied to the treatment of the microcystis aeruginosa polluted water body.

The invention belongs to the field of water purification and relates to a bacillus pumilus with efficient alga-lysing activity and application thereof. The bacillus pumilus NMCC46 with efficient alga-lysing activity is preserved in the China General Microbiological Culture Collection Center with the preservation No. as CGMCC No 3378 and the preservation date as Nov. 2, 2009. The bacillus pumilus NMCC46 has a very strong restraining effect to the growth and reproduction of microcystis aeruginosa, and has the algal inhibiting rate of 97.45% after 7 days. The fermentation supernate of the bacillus pumilus NMCC46 has an excellent alga-lysing effect at the final concentration of 0.5%. Alga-lysing active ingredients generated by the bacillus pumilus have excellent heat resistance and pH stability. Therefore, the bacillus pumilus NMCC46 can be used for controlling cyanobacterial bloom.

The invention relates to a polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa. Secondary effluent water of an urban sewage plant serves as a culture medium, the nontoxic microalgae and toxic cyanobacteria are subjected to joint culture in the secondary effluent water of the urban sewage plant, the density proportion between the initial nontoxic microalgae and toxic cyanobacteria is controlled to be (2:1)-(10:1), in the culture process, sodium nitrate and monopotassium phosphate serve as complementation sources of nitrogen and phosphorus respectively, the concentration of the culture medium is controlled to meet the conditions that total nitrogen (TN)=14.0-16.0 mg.L<-1>, total phosphorus (TP)=0.28-0.40 mg.L<-1>, and the proportion between nitrogen and phosphorus is (40-50): 1. Compared with the prior art, the polyculture type microalgae cultivation method is applicable to nontoxic microalgae culture, inhibition to growth of the microcystis aeruginosa is achieved, and the method is a safe, reliable and economic cultivation method; besides, according to the technical method, the secondary effluent water of the urban sewage plant serves as a culture medium source, and the function of deeply purifying sewage is achieved.

The invention discloses a method for extracting a microcystis aeruginosa extracellular polymer, The method comprises the following steps of taking an algae liquid of the microcystis aeruginosa, and performing centrifuging to obtain a dissolved type extracellular polymer; suspending the lower layer residue in a buffer solution, and performing centrifuging to obtain a loose attached type extracellular polymer; suspending the lower layer residue in an NaCl buffer solution, and adding sodium hydroxide, adjusting the pH value to be 11, and under the conditions of 4 DEG C and 80-120 rpm, performingstirring for 10-20 minutes, and then putting the mixture into a water bath at 40-60 DEG C for heating for 20-40 minutes; and performing centrifuging on the buffer solution to obtain a tightly combinedtype extracellular polymer. According to the method, the extracellular polymer is extracted by adopting a combination mode of "NaOH and heating" on microcystis aeruginosa, and the parameter extraction conditions are strictly controlled, so that a large amount of EPS in the algae be extracted, the algae cells can not be damaged, and pollution of the EPS is not caused, and therefore, the effectiveEPS component is extracted.

The invention discloses a microcystis aeruginosa research device which comprises a water tank, a motor and a lighting system, wherein the water tank is in the shape of an annular column with an upward opening; the motor is arranged in the center of circle of the water tank; an output shaft of the motor is provided with vertical supporting beams; the top ends of the supporting beams are provided with horizontal suspension arm beams; water pushing sheets are arranged below the suspension arm beams and extend into the water tank; and the lighting system is arranged at the periphery of the water tank. The device has the characteristic that a water body has basically consistent flow velocity during an experiment.

The invention provides brevundimonas sp. Brevundimonas sp. is named brevundimonas sp.J4, with collection number of CGMCC (China General Microbiological Culture Collection Center) NO.8976. The strain screened from the water body of the Taihu Lake has strong lytic effects on the dominant strain-microcystis aeruginosa in the cyanobacterial bloom, can be used for developing and producing novel algicides, and is finally applied to control of the fresh water cyanobacterial bloom.

The invention discloses an algistat, which is an absolute methanol crude extract of onion stalks, an absolute acetone crude extract of the onion stalks, an absolute petroleum ether crude extract of the onion stalks or an ethanol crude extract of the onion stalks. Compared with the prior art, the crude extracts from the onion stalks by the organic solvents perform an inhibitory effect to microcystis aeruginosa from high to low as follows: the inhibitory effect of absolute acetone is larger than that of 75% ethanol, the inhibitory effect of the 75% ethanol is larger than that of absolute petroleum ether, and the inhibitory effect of the absolute petroleum ether is larger than that of absolute methanol. The inhibition ratio of the absolute acetone pure extract of the onion stalks is enhanced along with the time, and a concentration effect is shown. The inhibition ratio of a high dose group on the fourth day can be up to 97.7%. EC50 (50% effective concentration) computed on the fifth day is 0.03g/L, which proves that the algal inhibition effect of the purified absolute acetone extract is more obvious than that before purification.

The invention belongs to the field of environmental microbial restoration, and discloses a method for synchronously dissolving algae/degrading algal toxins by using a microbial combined preparation. According to the method, a Pseudomonas aeruginosa fermentation liquid is utilized to dissolve microcystis aeruginosa in the logarithmic growth phase, and a Pycnoporus sanguineus spore liquid is utilized to degrade residual microcapsule algal toxins in the alga dissolution system, thereby achieving the goals of synchronously removing algae and degrading metabolites. The method is simple and is low in cost. The fermentation time is 48 hours, and the alga dissolution time is 7 days. When the degradation time is 7 days, the alga dissolution effect reaches 83.31%, and the degradation efficiency of microcapsule algal toxins reaches 67.63%, thereby achieving the goal of synchronously removing algae and degrading metabolites. The method has high practical application value, and provides references for solving the problems of lake/reservoir water eutrophication and drinking water deep treatment.