48 results about "Genus Lysinibacillus" patented technology

The invention provides lysinibacillus fusiformis, the collection number of which is CGMCC (China General Microbiological Culture Collection Center) NO.8835. The invention further provides a microbial agent which contains thalli and a culture medium, wherein the thalli comprises the lysinibacillus fusiformis the collection number of which is CGMCC NO.8835. The invention further provides applications of the lysinibacillus fusiformis and the microbial agent in preparing a microbial fertilizer. Through the technical scheme, the fertilizer efficiency of a phosphorous fertilizer can be more effectively increased.

The invention discloses a COD (chemical oxygen demand) degradation microbial inoculant which comprises the following components in percentage by volume: 15-20% (preferably 20%) of Bacillus subtilis, 10-15% (preferably 15%) of Bacillus amyloliquefaciens, 10-20% (preferably 10%) of Bacillus anthracis, 10-15% (preferably 10%) of Staphylococcus pasteuri, 10-15% (preferably 10%) of Aerococcus viridans, 10-20% (preferably 10%) of Lactobacillus casei, 10-15% (preferably 10%) of Lysinibacillus fusiformis and 15-20% (preferably 15%) of Enterococcus. The COD degradation microbial inoculant effectively lowers the turbidity of the water body, and enhances the weight concentration of the water body on the premise of implementing environmental protection and saving water. The invention also discloses a preparation method and application of the COD degradation microbial inoculant.

The invention relates to a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment, belonging to the technical field of environment-friendly water treatment. The invention provides Lysinibacillus fusiformis of which the collection number is CGMCC NO.7520. The invention also provides a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment. The screened algicidal bacteria has a certain dissolution action on the dominant alga strain microcystis aeruginosa in blue algae bloom, and the algicidal bacteria TR3 can be obtained by separation. Under the optimum conditions, the removal rate of the algicidal bacteria TR3 for microcystis aeruginosa within 96 hours can reach 97.18%, so the algicidal bacteria TR3 has very ideal alga dissolution effect; and thus, the algicidal bacteria TR3 can better display the alga dissolution characteristic in a natural water body, and has wider application prospects in large-area water body blue algae bloom pollution treatment.

The invention discloses a lysinibacillus fusiformis strain and use thereof. The lysinibacillus fusiformis strain is lysinibacillus, named after lysinibacillus fusiformis GZU-Lys01, and preserved in the Chinese typical culture preservation centre on December 31, 2019; and the preservation number is CCTCC NO:M20191140. The lysinibacillus fusiformis (Lysinibacillus fusiformis strain) GZU-Lys01 in theinvention can effectively dissociate difficultly dissolved and / or undissolved phosphorus, potassium, nitrogen and the like in coal gangue, and convert into nutritional components absorbed by plants,such as available phosphorus, rapidly available potassium and hydrolyzable nitrogen; particularly, the GZU-Lys01 can effectively convert difficultly dissolved phosphorus and / or undissolved phosphorusin the coal gangue into available phosphorus; simultaneously, the GZU-Lys01 also can effectively dissociate other phosphorus-containing minerals; when the GZU-Lys01 is used for preparing a coal ganguemicrobial fertilizer, other phosphorus-containing minerals can be doped properly; the phosphorus source for preparing the microbial fertilizer is increased; and, due to the GZU-Lys01 strain, coal gangue resource recycling becomes possible.

Disclosed is an immunopotentiating composition comprising an effective immunostimulating substance which can activate natural immunity and a subsequent immunity mediated by lymphocytes without causing any damage to cells. Specifically disclosed is an immunopotentiating composition which is characterized by comprising, as an active ingredient, an immunostimulating substance produced by decomposing at least one bacterium selected from an MRE symbiotic bacteria group consisting of Bacillus sp. (FERM BP-11209), Lysinibacillus fusiformis (FERM BP-11206), Bacillus sonorensis, Lysinibacillus sp. (FERM BP-11207) and Comamonas sp. (FERM BP-11208).

The invention discloses a lysinibacillus fusiformis for degrading a dairy cow faeces sour matter and application of the lysinibacillus fusiformis. Lysinibacillus fusiformis LF-N1 provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) now with a preservation number being CCTCC No: M 2017289. The lysinibacillus fusiformis LF-N1 can effectively degrade short-chain sour fatty acid compounds contained in biomass substrates including sawdust when being therein, is suitable for preparing an efficient microbial agent product in a dairy cow breeding bed ecological breeding mode, and can also efficiently degrade two organic pollutants, including indole and skatole in livestock and poultry industry, thus having very good application prospect in the aspects of ecological breeding, environmental governance and the like.

The invention discloses a composite microbial inoculum of lysinibacillus and bacillus subtilis and a preparation method thereof, and belongs to the technical field of agricultural microbes. The composite microbial inoculum is prepared by blending and confecting bacillus subtilis R31 fermentation broth and lysinibacillus fusiformis LW-3 fermentation broth, wherein the collection number of the lysinibacillus fusiformis LW-3 is CGMCC (China General Microbiological Culture Collection Center) No. 16228; and the mixing proportion of the bacillus subtilis R31 fermentation broth to the lysinibacillusfusiformis LW-3 fermentation broth is (1 to 10) to (10 to 1). The composite microbial inoculum provided by the invention has obvious growth-promotion, antibacterial and disease-prevention effects on aplant, can obviously inhibit the occurrences of diseases of bacterial wilt, blight and root rot and the like, improves a yield and quality, has the characteristics of being safe, efficient, nontoxicand pollution-free, can effectively reduce the use of a chemical fertilizer and a pesticide, relieves environmental pollution pressure, and assists agricultural sustainable development.

The invention discloses a microbial deodorant and a preparation method thereof. The microbial deodorant is specially used for removing putrid odors generated by NH3 and H2S and comprises lysinibacillus fusiformis J2 which can absorb and utilize H2S and a bacterial strain with nitrosation ability. The bacterial strain with nitrosation ability is one of pseudomonas YF1 or sinorhizobium fredii YS2 or a mixed bacterial strain of YF1 and YS2. The bacterial strain concentrations of the pseudomonas YF1, sinorhizobium fredii YS2 and lysinibacillus fusiformis J2 are above order of magnitudes of 1*10<8> / ml. The deodorant disclosed by the invention has the advantages that the microbial deodorant has the utilizing effect to NH3 and H2S which generate putrid odors, and meanwhile further can obstruct emission of putrid odors and has a remarkable deodorizing effect. The preparation method of the microbial deodorant is simple, flow in process flow, low in cost and convenient to use, and the microbial deodorant can be stored at normal temperature with a long period of validity.

The invention discloses lysinibacillus fusiformis and an application thereof in land reclamation and ecological reconstruction in a mining area. The strain is the lysinibacillus fusiformis, and has acollection number of CGMCC No. 9799. The strain is capable of improving the nutrient content of land reclamation soil in the mining area, and has obvious effects for improving the content of organic matters, alkali-hydrolyzale nitrogen, rapidly available potassium and available phosphorus in the land reclamation soil in the mining area. Moreover, further experiments prove that, the lysinibacillusfusiformis is capable of further improving the biomass of crops, and for culture of wheat or oilseed rapes, the biomass of crops in the land reclamation soil in the mining area can be improved; particularly important, due to use of the lysinibacillus fusiformis, the community structure of soil microorganisms can be effectively and obviously changed, the microbial diversity of the soil is improved,and the quantity of microbes is effectively increased. Therefore, remediation of the microbial community in the soil is accelerated, and the lysinibacillus fusiformis can be widely applied to remediation of the land reclamation soil in the mining area.

The invention discloses a Lysinibacillus fusiformis strain and an enzyme preparation, and application of the same to degradation of pesticide residues. The invention provides the Lysinibacillus fusiformis strain FY-7, with an accession number of CGMCC No. 13523. The invention also provides screening, induction and domestication of the strain and a preparation method for a pesticide residue-degrading active enzyme. Chlopyrifos is used as a carbon source for screening of the target strain, and then the target strain is subjected to induction and domestication with a single pesticide and a mixed pesticide. The strain is subjected to fermentation, then bacterial cells are collected, broken and centrifuged, and the obtained supernatant can be used for preparing the pesticide residue-degrading enzyme preparation. The prepared pesticide residue-degrading enzyme preparation can be used for degrading pesticide residues on vegetable and fruit and has application potential in degradation of pesticide residues on vegetable and fruit.

The invention provides lysinibacillus fusiformis and application thereof, and belongs to the technical field of agricultural microorganisms. The classified name of the lysinibacillus fusiformis is lysinibacillus fusiformis LW-3, and the preservation number is CGMCC No.16228. The lysinibacillus fusiformis LW-3 with the preservation number being CGMCC No.16228 has the functions of phosphate solubilization and potassium dissolving, soil is regulated, soil fertility can be effectively improved, the usage amount of chemical fertilizer is decreased, the soil environment is improved, and good growthof plants is promoted.

The invention discloses a method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quick lime. The quick lime is used for improving the effect of mineralizingand fixing the heavy metals by microorganisms, the quick lime can generate calcium ions and hydroxyl ions when reacting with water, the generated calcium ions can provide a calcium source required bycalcification of the microorganisms, and the hydroxyl ions can improve the pH of a tailing solidification system; the lysinibacillus fusiformis simultaneously cement tailing particles with the quicklime while generating more calcium carbonate crystals to fix the heavy metals, so that the concentration of lead ions, copper ions, zinc ions and nickel ions in tailing leachate is reduced, the possibility that heavy metals in the tailings are leached and flow into nearby soil and rivers is greatly reduced, and large-scale environmental pollution is avoided; and meanwhile, a solidified body has certain compressive strength, so that a feasible idea is provided for resource utilization of the tailings.

The invention discloses a preparation method of a coal gangue microbial fertilizer, wherein the coal gangue microbial fertilizer is prepared by dissociating coal gangue serving as a raw material by using lysinibacillus fusiformis GZU-Lys01. According to the method, waste coal gangue is recycled, and the method is simple in preparation process, mild in condition, green and environment-friendly.

The invention belongs to the technical field of biology, and relates to bacillus for biodegradation of uric acid, the bacillus can generate enzyme for catalytic degradation of uric acid, and the bacillus is lysinibacillus fusiformis YC-01 strain. The invention also relates to a lysinibacillus fusiformis preparation for degrading uric acid. The lysinibacillus fusiformis preparation contains one or more of bacterial cells, spores and crude enzyme of the bacillus. Research results show that the bacillus for biodegrading uric acid and the enzyme produced by the bacillus are safe to human bodies, can efficiently biodegrade uric acid, and have important application prospects in the aspects of removing uric acid and treating human hyperuricemia and corresponding complications.

The invention discloses a method for mineralizing heavy metals in a leachate of garbage burning bottom ash by Lysinibacillus fusiformis. Lysinibacillus fusiformis is inoculated into the garbage burning bottom ash leachate containing urea and calcium chloride, shaking culture in a shaker at 20 to 30 DEG C at a shaking speed of 100 to 150 rpm is carried out, so that mineralization of heavy metals inthe garbage burning bottom ash leachate is realized. According to the invention, through repairing and treatment of the heavy metals in the garbage burning bottom ash leachate by the Lysinibacillus fusiformis under the conditions of urea and calcium chloride, the method has the advantages of simple process, convenient operation, obvious effect, low treatment cost, wide application range, no secondary pollution and other advantages. Analysis on the repaired leachate after degradation by ICP-OES shows that the removal rates of Pb, Cd and As are 96.0%, 98.9% and 92.8%, respectively.

The invention provides preparation and application of a netropsin lysinibacillus fusiformis selenium-rich inoculant, and relates to the field of organic selenium preparations for animal breeding. The netropsin lysinibacillus fusiformis is able to enrich inorganic selenium with high concentration, and mycoprotein per 10 kg (dry weight) is able to enrich 20,000 mg selenium in total; inoculant powder prepared through the produced selenium-rich inoculant containing selenium-enriched protein contains more than 3,000 mg / kg of selenium in total and can be directly fed to animal to supplement selenium element, thus the meat quality of the bred animal can be improved; toxicity experiment verifies that netropsin lysinibacillus fusiformis does not cause adverse reaction in tested animal; the netropsin lysinibacillus fusiformis can be used for producing phytase, protease and glycosidase, so that the digestion of the animal can be improved, and the feed utilization rate is increased.

The invention relates to a Lysinibacillus fusiformis ZJB-17006 and application thereof. The preparation of L-amino acids by catalyzing N-phenylacetyl-DL-amino acid has an enantioselectivity value reaching 99%, and the preparation of 2-amino-4-[hydroxy(methyl)phosphoryl]-L-butyric acid by catalyzing 2-N-phenylacetyl-4-[hydroxy(methyl)phosphoryl]-DL-butyric acid has an enantioselectivity value reaching 99%.

The invention provides application of lysinibacillus fusiformis with an MICP function in greening restoration of tailings. The application comprises the following steps: taking quantitative slag around mine tailings in a mining area according to regional ecological environment characteristics, taking the slag back to a laboratory for liquid Yao culture, and screening microorganisms with an MICP function; according to a color developing agent result and a 16S analysis result, confirming that one strain is lysinibacillus fusiformis, and confirming the function of decomposing urea to induce calcium carbonate precipitation of the lysinibacillus fusiformis; determining the MICP function of the strain according to physicochemical properties of the strain, especially calcium carbonate precipitation reaction; on the basis of the MICP solidified alien soil regreening composite ecological restoration technology, adopting alien soil for planting plants to improve the ecological environment of the mining area, and realizing the tailing regreening effect. The application has a good tailing solidification effect, can solidify slag, has a promotion effect, namely a seed enhancement effect, on regional plant seed germination, and is beneficial to reduction of regional mine restoration technology cost.

The invention discloses application of lysinibacillus fusiformis in removing hexavalent chromium in eutrophic wastewater, and belongs to the technical field of microorganisms. The strain is preservedin China General Microbiological Culture Collection Center; the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.10053, the preservation name is Lysinibacillus fusiformis WTXJ1-4, and the preservation date is November 25, 2014. Through single factor test and orthogonal test optimization, the appropriate biological adsorption conditions of the strain for the hexavalent chromium in the eutrophic wastewater are determined as follows: the pH value is 2.0, the initial concentration of potassium dichromate is 50 mg / L, the adsorption time is 24 hours, the dosage of wet thalli is 1.0 g / L, the adsorption temperature is 34 DEG C, and the rotating speed is 160 r / min. And when the optimized adsorption conditions are balanced, theremoval rates of the WTXJ1-4 living bacteria on the hexavalent chromium and total chromium in the eutrophic wastewater respectively reach 91.6%-94.8% and 42.2%-43.8%. According to the present invention, the Lysinibacillus fusiformis WTXJ1-4 living bacteria have strong adsorption-reduction property, strong tolerance and strong resistance on the hexavalent chromium in the eutrophic wastewater, wherein the action ranges are as follows: the concentration of potassium dichromate is 0-100 mg / L, the concentration of potassium dichromate is 100-300 mg / L, and the concentration of potassium dichromate is 300-500 mg / L.

The invention discloses lysinibacillus fusiformis for rhizoctonia solani inhibition. A microbial strain and agricultural chemical products thereof are provided and are nontoxic to human and animals, environmentally friendly and effective in rhizoctonia solani prevention. Microbial bactericides for rhizoctonia solani prevention and treatment are diversified.

Disclosed is an immunopotentiating composition comprising an effective immunostimulating substance which can activate natural immunity and a subsequent immunity mediated by lymphocytes without causing any damage to cells. Specifically disclosed is an immunopotentiating composition which is characterized by comprising, as an active ingredient, an immunostimulating substance produced by decomposing at least one bacterium selected from an MRE symbiotic bacteria group consisting of Bacillus sp. (FERM BP-11209), Lysinibacillus fusiformis (FERM BP-11206), Bacillus sonorensis, Lysinibacillus sp. (FERM BP-11207) and Comamonas sp. (FERM BP-11208).

The invention discloses lysinibacillus fusiformis H16 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Lysinibacillus fusiformis H16, and the preservation number of the Lysinibacillus fusiformis H16 is CCTCC (China Center for Type Culture Collection) NO: M 2021179. The Lysinibacillus fusiformis H16 disclosed by the invention can inhibit the growth of the Ustilaginoidea virens strain YY7850, which is an example that the Lysinibacillus fusiformis is found to have antagonism to the Ustilaginoidea virens pathogenic bacteria for the first time, and due to the fact that the strain H16 has the capacity of inhibiting the Ustilaginoidea virens pathogenic bacteria, the strain has the potential of being developed into a rice false smut biocontrol bacterium.

The invention provides a method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria. The modified clay is cetyl trimethyl ammonium bromide modified sodium montmorillonite. The algal inhibiting bacteria can be lysinibacillus fusiformis J8 which is called a J8 strain for short. The J8 strain is preserved in the Guangdong microbial culture collection center on December 13th, 2017, and the preservation number is GDMCC NO. 60298. Experimental data shows that the combined application of the modified clay and the algal inhibiting bacteria (J8) has an obvious inhibition effect on algal cells, the continuous algal inhibition time can reach 168 hours, and the rebound of the number of the algae cells is avoided. The modified clay used in the method is wide in source,simple to prepare, low in cost and easy to operate; the modified clay and the algal inhibiting bacteria are combined so that the red tide can be more thoroughly inhibited; a method for inhibiting thered tide by combining the modified clay and the algal inhibition bacteria, no secondary pollution is generated, and the method is an environment-friendly method.

The invention discloses a vitamin B-producing 12 strain, the preservation number of which is GDMCC No.60674. The invention also discloses the vitamin B-producing 12 The strain GDMCC No.60674 was used for the production of vitamin B 12 Applications. vitamin B 12 The strain GDMCC No.60674 can ferment and produce vitamin B through liquid static culture 12 , and the strain belongs to the genus Lysinibacillus, and there is currently no Lysinibacillus producing vitamin B 12 Therefore, the strain GDMCC No.60674 has good industrial application value.

The invention discloses a lysinibacillus fusiformis and its application in ecological reconstruction of mine area reclamation. The bacterium is Lysinibacillus fusiformis, and its preservation number is CGMCC No.9799. The strain can increase the nutrient content of reclaimed soil in mining areas, and has a significant effect on increasing the content of organic matter, alkaline nitrogen, available potassium and available phosphorus in reclaimed soil in mining areas; and further experiments have shown that the spindle-shaped lysine bacillus It can also increase the biomass of crops, whether it is for the cultivation of wheat or rapeseed, it can increase the biomass of crops in reclaimed soil in mining areas; it is particularly important that the use of spindle-shaped lysine bacillus can effectively and significantly change soil microorganisms The community structure of the soil can improve the diversity of soil microorganisms, effectively increase the number of microorganisms, thereby speeding up the restoration of soil microbial communities, and can be widely used in the restoration of reclaimed soil in coal mining areas.

The invention relates to application of siderophore plant-growth-promoting rhizobacteria, in particular to application of lysinibacillus fusiformis to prevention and / or treatment of iron chlorosis of cinnamomum camphora. The fermentation method of the siderophore plant-growth-promoting bacteria comprises the following steps of: transferring lysinibacillus fusiformis into an LB liquid culture medium with the pH value of 8.0-10.0, and carrying out shake culture under the conditions of 26-30 DEG C and 140-160r / min until the logarithmic phase to obtain a seed bacteria liquid; inoculating the seed bacteria liquid into a fresh LB liquid culture medium with the pH value of 8.5-10.5 according to the inoculum size of 2% by volume, and performing shaking culture and fermentation to obtain fermentation liquid; and calculating the effective viable count in the fermentation liquid, then centrifuging the fermentation liquid, discarding supernate, diluting the fermentation liquid with water to obtain a fermentation diluent with the viable count of 10<9>CFU / mL, and then irrigating or spraying the fermentation diluent into soil for planting cinnamomum camphora. The siderophore plant-growth-promoting rhizobacteria can be used for effectively preventing and treating iron chlorosis of cinnamomum camphora, and is simple in process, environment-friendly, long in lasting period and low in cost.