Lysinibacillus fusiformis strain and use thereof
A lysine bacillus and spindle-shaped technology, which is applied in the field of the spindle-shaped bacillus lysine and its use, can solve the problems of toxic substances flowing into the soil, occupying land resources, heavy metal pollution, etc., and achieves the ability to improve, improve Phosphorus absorption coefficient, stress relief effect
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[0027] The separation of spindle-shaped lysine bacillus GZU-Lys01 comprises the following steps:
[0028] 1. Isolation and screening of functional strain GZU-Lys01
[0029] 1) Collection of samples
[0030] (1) The sample was collected from a coal gangue hill in a coal production area, and the fully weathered gangue sample and nearby soil samples were selected, and the collected samples were put into a collection bag;
[0031] (2) crush and sieve the sample collected, and collect a 100-mesh sample;
[0032] (3) Store the samples in a refrigerator at 4°C for later use.
[0033] 2) Screening of functional strains
[0034] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;
[0035] (2) Shake in a constant temperature shaker at 180r / min, 27°C for 30min;
[0036] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10 -2 , 1.0×10 -3 , 1.0×10 -4 , 1.0×10 -5 , 1.0×10 -6 , 1.0×10 -7 .
[0037] (4) ...
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