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Lysinibacillus fusiformis strain and use thereof

A lysine bacillus and spindle-shaped technology, which is applied in the field of the spindle-shaped bacillus lysine and its use, can solve the problems of toxic substances flowing into the soil, occupying land resources, heavy metal pollution, etc., and achieves the ability to improve, improve Phosphorus absorption coefficient, stress relief effect

Active Publication Date: 2020-11-06
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All coal production areas basically store coal gangue in the open air, and the massive accumulation of coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; weathering and spontaneous combustion of coal gangue will release toxic gases, generate dust, and cause heavy metal pollution. Pollution; the rain washes the gangue, causing the toxic substances in the gangue to flow into the soil and pollute the water quality
In short, the massive accumulation of coal gangue has caused waste of resources and serious environmental pollution, so it is of great significance to develop new methods of resource utilization of coal gangue

Method used

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  • Lysinibacillus fusiformis strain and use thereof
  • Lysinibacillus fusiformis strain and use thereof
  • Lysinibacillus fusiformis strain and use thereof

Examples

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Embodiment

[0027] The separation of spindle-shaped lysine bacillus GZU-Lys01 comprises the following steps:

[0028] 1. Isolation and screening of functional strain GZU-Lys01

[0029] 1) Collection of samples

[0030] (1) The sample was collected from a coal gangue hill in a coal production area, and the fully weathered gangue sample and nearby soil samples were selected, and the collected samples were put into a collection bag;

[0031] (2) crush and sieve the sample collected, and collect a 100-mesh sample;

[0032] (3) Store the samples in a refrigerator at 4°C for later use.

[0033] 2) Screening of functional strains

[0034] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;

[0035] (2) Shake in a constant temperature shaker at 180r / min, 27°C for 30min;

[0036] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10 -2 , 1.0×10 -3 , 1.0×10 -4 , 1.0×10 -5 , 1.0×10 -6 , 1.0×10 -7 .

[0037] (4) ...

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Abstract

The invention discloses a lysinibacillus fusiformis strain and use thereof. The lysinibacillus fusiformis strain is lysinibacillus, named after lysinibacillus fusiformis GZU-Lys01, and preserved in the Chinese typical culture preservation centre on December 31, 2019; and the preservation number is CCTCC NO:M20191140. The lysinibacillus fusiformis (Lysinibacillus fusiformis strain) GZU-Lys01 in theinvention can effectively dissociate difficultly dissolved and / or undissolved phosphorus, potassium, nitrogen and the like in coal gangue, and convert into nutritional components absorbed by plants,such as available phosphorus, rapidly available potassium and hydrolyzable nitrogen; particularly, the GZU-Lys01 can effectively convert difficultly dissolved phosphorus and / or undissolved phosphorusin the coal gangue into available phosphorus; simultaneously, the GZU-Lys01 also can effectively dissociate other phosphorus-containing minerals; when the GZU-Lys01 is used for preparing a coal ganguemicrobial fertilizer, other phosphorus-containing minerals can be doped properly; the phosphorus source for preparing the microbial fertilizer is increased; and, due to the GZU-Lys01 strain, coal gangue resource recycling becomes possible.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a spindle-shaped lysine bacillus strain and its application. Background technique [0002] my country is a large country of coal production, and coal occupies an important position in my country's energy structure. The coal gangue produced in the process of coal mining and washing has become the largest industrial solid waste because it has not been effectively utilized. All coal production areas basically store coal gangue in the open air, and the massive accumulation of coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; weathering and spontaneous combustion of coal gangue will release toxic gases, generate dust, and cause heavy metal pollution. Pollution: Rainwater scours coal gangue, causing toxic substances in coal gangue to flow into the soil and pollute water quality. In short, the massive accumulation of coal gangue ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/40C12R1/01
CPCC12N1/20C05G3/44C12R2001/01C12N1/205
Inventor 谢承卫高弦王应兰吉俐李小军董丽敏
Owner GUIZHOU UNIV
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