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Special shewanella and application thereof

A technology of Shewanella and bacterial agents, applied in the field of Isoshewanella, can solve environmental problems such as pollution, waste of resources, etc., and achieve the effects of simple process, improved phosphorus absorption coefficient, and environmental friendliness

Pending Publication Date: 2022-01-07
中国烟草总公司贵州省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of coal gangue has caused waste of resources and serious environmental pollution, so it is of great significance to develop new methods of resource utilization of coal gangue

Method used

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  • Special shewanella and application thereof
  • Special shewanella and application thereof
  • Special shewanella and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Isolation and screening of functional strain GZT-Ali01

[0036] 1) Collection of samples

[0037] (1) Samples were collected from moldy plastered phosphogypsum samples. Select plastered phosphogypsum samples with obvious damp and moldy conditions in the construction site, and put the collected samples into collection bags.

[0038] (2) The collected samples were crushed and sieved, and the 100-mesh samples were collected.

[0039] (3) Store the samples in a refrigerator at 4°C for later use.

[0040] 2) Screening of functional strains

[0041] (1) Accurately weigh 1 g of the sample into a sterilized 250 mL Erlenmeyer flask, and add 99 mL of sterile water.

[0042] (2) Shake for 30 minutes in a constant temperature shaker at 180r / min at about 27°C.

[0043] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10-2, 1.0×10-3, 1.0×10-4, 1.0×10-5, 1.0×10-6, 1.0×10-7 .

[0044] (4) Take 100uL of each concentration and spread on 3 solid m...

Embodiment 2

[0050] Example 2. The drawing of the growth curve of the functional strain

[0051] 1) Preparation of seed solution. The strain was inoculated in LB liquid medium with an inoculation loop, and cultivated to the logarithmic phase at 35°C.

[0052] 2) Vaccination. Take 10 mL of the above seed solution and inoculate it into 200 mL of liquid LB medium, mix well and take 5 mL of the mixed solution and put them in 18 marked sterile test tubes.

[0053] 3) Cultivate. Place the inoculated test tubes at 35°C and incubate in a shaker at 180r / min.

[0054] 4) Measure. Use uninoculated LB liquid culture medium as a blank, and conduct photoelectric turbidimetry with the sample to be tested at a wavelength of 600nm.

[0055] Measurement results such as figure 1 , the strain enters the logarithmic phase after 15h in the culture process of LB liquid medium, enters the stable phase from 17h to 30h, and enters the decay phase after 35h.

Embodiment 3

[0056]Example 3. Identification of functional strains

[0057] 1) Colony appearance

[0058] Such as figure 2 , it can be seen from the figure that the colony morphology of the strain in the solid medium of complete nutrient agar is off-white, opaque, moist, viscous, and the colony morphology is irregular.

[0059] 2) Microscopic morphology of bacteria

[0060] After Gram staining, the microscopic appearance of the bacteria was as follows: image 3 , it can be seen from the figure that the bacterial strain is observed under a microscope, the thalline is granular, a single grain exists, and the thalline has no spores, and it is identified as a Gram-negative bacterium.

[0061] 3) Scanning electron microscope morphology of bacteria

[0062] Such as Figure 4 , the shape of the electron microscope of the bacteria is rod-shaped, which is consistent with the characteristic image of the optical microscope. The length is about 950nm. The surface of the bacteria is shriveled an...

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Abstract

The invention discloses a special shewanella and application thereof. The special shewanella is preserved in the China Center for Type Culture Collection on July 8, 2021, the preservation number is CCTCC NO: M 2021844, and the special shewanella is classified and named as fermented seafood special shewanella GZT-Ali01. The special shewanella GZT-Ali01 can dissociate nitrogen, phosphorus, potassium and other components in the coal gangue or the phosphogypsum-doped coal gangue, convert the components into hydrolytic nitrogen, available phosphorus, quick-acting potassium and other nutritional components which can be absorbed by plants, and particularly can effectively dissociate insoluble phosphorus and / or insoluble phosphorus components in the coal gangue or the phosphogypsum-doped coal gangue. And the process flow for dissociating the coal gangue or the phosphogypsum-doped coal gangue by adopting the strain disclosed by the invention is also simple, the dissociation effect is obvious, the cost is low, and the method is green and environment-friendly.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a kind of Shewanella isobacteria and its application. Background technique [0002] Coal gangue is a kind of black rock with high ash content, low carbon content, low calorific value, and harder than coal that is associated with coal seams during coal formation and removed during the production of raw coal in coal mines. In the process of coal mining and processing, about tens of millions of tons of coal gangue are produced every year. The accumulation of a large amount of coal gangue not only occupies land resources, but also pollutes the environment. The composition of coal gangue is complex, and due to the different places of origin, the compositional differences will be relatively large, and the properties are also different, which brings great difficulties to the comprehensive utilization of coal gangue. So far, coal gangue has not been effectively utilized and has become ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/80C12R1/01
CPCC12N1/20C05F11/08C05G3/80
Inventor 谢承卫张景宁李小军盛定红
Owner 中国烟草总公司贵州省公司
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