118 results about "Shewanella oneidensis" patented technology

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

The invention discloses application of a Harmine derivative to preparation of antibacterial medicines. The bacteria is selected from Acinetobacter, Bacillus, Campylobacter, Chlamydia, Chlamydia trachomatis, Clostridium, Citrobacter, Escherichia, enterohemorrhagic escherichia coli, enteric bacteria, Enterococcus, Francisella, Haemophilus, helicobacter, Klebsiella Bacillus, Lester monocytogenes, Moraxella, Mycobacterium, Neisseria, proteus, Pseudomonas, Salmonella, shewanella oneidensis, Shigella, Stenotrophomonas, Staphylococcus, Streptococcus and Yersinia.

The invention belongs to the technical field of biology, and relates to a preparation method and application of shewanella spp-nanometer ferrous sulfide capsules. The method comprises the steps that an LB culturing medium and an M9 culturing medium are prepared, shewanella spp is inoculated to the LB culturing medium for culturing, and bacterial liquid is obtained; the LB culturing medium and theM9 culturing medium are mixed, sodium lactate, calcium chloride and magnesium sulfate are added, and reaction buffering liquid is obtained; then, the bacterial liquid is centrifuged, bacterial sludgeis added into the reaction buffering liquid until the OD 600 value is 0.05-5; then, a ferric chloride solution and a sodium thiosulfate solution are added into the reaction buffering liquid after inoculation of the bacterial sludge, and after culturing, centrifugation, washing and resuspending, the shewanella spp-nanometer ferrous sulfide capsules are obtained; in the process of synthesizing the capsules, the activity of the biological metabolism mechanism is fully utilized, the raw materials are simple and cheap, the application cost is low, and quantization production can be achieved; multi-period efficient removal for hexavalent chromium is efficiently achieved, and a new method is provided for efficiently processing heavy metal pollution.

The invention discloses shewanella and application thereof in a microbiological fuel cell, and relates to the technical field of biology. The shewanella is Shewanella marisflavi EP1 which is preserved in 'China Center for Type Culture Collection' in January 2009 with the preservation number of CCTCC M 209016. The shewanella is characterized in that: the shewanella is gram-negative; the thalli are rod-shaped and straight; two ends are circular; the diameter is 0.3 to 1.0 micro; the length is 2.5 to 6.0 micros; the thalli can singly exist or are arranged in pair or in a short-chain shape; and the thalli have autogenetic flagella and can move. The shewanella is separated from offshore ocean sediments, has wide salt ion concentration tolerance activity, and can generate power in electrode liquid at the concentration of up to 8 percent. Therefore, the internal resistance of the microbiological fuel cell is effectively reduced.

The invention belongs to the field of biological nano-materials, and specifically relates to a preparation method of biological nano-ferrous sulfide. The preparation method comprises the specific steps: firstly, a phosphate-ferric sustained release system is constructed; secondly, shewanella oneidensis MR-1 is inoculated into an LB culture medium, subjected to activation culture, and then inoculated into the phosphate-ferric sustained release system under aseptic and anaerobic conditions, and when the OD600 value of the final concentration of the shewanella oneidensis MR-1 in the system is 0.1-2, inoculation with the shewanella oneidensis MR-1 stops; and then sodium thiosulfate mother liquor is added, and the biological nano ferrous sulfide is obtained by culturing, centrifuging and washing. The novel phosphate-ferric sustained release system is constructed to realize the quantitative control of ferric iron. A series of nano-ferrous sulfide with different catalytic properties can be controllably bio-synthesized by adjusting the adding amount of the sulfur source in synthesis, the catalytic performance is effectively regulated, and a new way is opened up for regulation of the catalytic performance of the bio-synthesized nano-material.

The invention discloses a Brevibacillus laterosporu with function in quickly decomposing nitrite nitrogen and bacteriostasis function and an application thereof. The screening strain is Brevibacillus laterosporu YB-02, the strain collection number is CGMCC-No. 9757. The strain provided by the invention has a capacity of effectively decomposing nitrite nitrogen in water, has cholate resistance and trypsin resistance, and further has higher inhibiting effects to mariculture pathogenic bacteria like vibrio splendidus, shewanella smarisflavi and edwardsiella tarda. Therefore, the strain provided by the invention can be used for developing probiotics products for mariculture water purification and bacterial inhibition, the antibiotics abuse is avoided, the fishing disease is prevented and treated effectively, the quality and yield of marine food products are improved; the strain has effective, fast and safe water purification effect to acute water pollution, and therefore, the strain is wide in application prospect.

The invention discloses arginine decarboxylase and application thereof and belongs to the technical field of biological engineering. Shewanella putrefaciens is cloned by adopting genome database mining and homologous sequence alignment and other virtual screening means and combining with a molecular biological means to obtain an arginine decarboxylase gene. The molecular biological means, process optimization and the like are adopted, the enzyme production capacity of arginine decarboxylase production strains is improved, and a platform for efficiently producing gamatine through biological catalysis is established by optimizing a catalytic system. Crude enzyme liquid obtained through fermentation is purified and then is converted, a reaction system is clear in composition, and extraction and purification of follow-up products are promoted. Under the condition of 37 DEG C, the decarboxylase has high activity, meanwhile is beneficial to the growth of escherichia coli of host cells, and at the temperature, the reaction speed is improved greatly, a conversion period should be 24 hours, the gamatine yield can be up to 61-71 g / L, and the conversion rate can be up to 68-82%.

The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: E. coli W3110, a human related enterobacterium and Shewanella oneidensis MR-1, an environmentally important bacterium with versatile metabolism.

The invention relates to the technical field of biological energy, and discloses a recombinant plasmid, recombinant shewanella and an electricity generation method of a microbial fuel cell (MFC). A promoter suitable for the shewanella is selected to be connected with an exogenous electron carrier flavins gene, so that the shewanella is enabled to generate more flavis, and the electrochemical performance of the MFC is improved; furthermore, a mutant strain is combined with a porin oprF gene, so that the formation of a biofilm is further improved, the going in and out of the flavins in the shewanella can be promoted, the electron transfer rate is increased, and electrons are better transmitted. Furthermore, biocompatible graphene oxide (GO) is used and can be self-assembled into the three-dimensional multilayer biofilm together with the shewanella, so that the thickness of the biofilm and the anode biocapacity are further greatly improved; the operation is simple and fast, and the electrochemical performance of the MFC is effectively improved.

The invention discloses a method for synthesizing shewanella halitios into god nanoparticles and application of the gold nanoparticles. The method comprises the following steps that firstly, shewanella halitios Z4 cultivated to a stable period is centrifugally collected, the shewanella halitios Z4 is cleaned by deionized water, and then the shewanella halitios Z4 is prepared into a bacterium suspension; secondly, the bacterium suspension in the step one is added into a chloroauric acid solution, sodium lactate is added as an electron donor, a table concentrator carries out shake cultivation, and a nanogold solution is obtained through the reaction; thirdly, the nanogold solution in the step two is centrifugally collected, and is dried for 12 hours to 36 hours at the temperature of 60 DEG C to 90 DEG C to obtain the gold nanoparticles. According to a green synthesis method, no large type equipment is needed, the method is simple, the condition is moderate, hydrogen does not need to be added as the electron donor, cost is low, and the method is safe. The synthetic gold nanoparticle particles can be used as catalysts to catalyz degradation of nitroaniline, and the method can be applied to removal of environment pollutants.

The invention relates to a method for increasing genetic transformation of improved Shewanella oneidensis MR-1 and belongs to the field of mutation or genetic engineering. The method comprises the following steps: adding nanometer titanium dioxide grains into a solution formed by mixing a donor bacterium Escherichiacoli WM3064 and a recipient bacterium S.oneidensis MR-1 of a foreign gene in an equal proportion way, and then statically culturing. According to the method, the foreign gene is effectively boosted to transfer from the donor bacterium to the recipient bacterium and the genetic transformation efficiency of the foreign gene to the S.oneidensis MR-1 is obviously increased. The invention also provides a test basis to the new application of a nanometer material in the field of genetic engineering. The method has a potential practical use value and meets the demand of the national strategy of sustainable development.

The invention discloses a use of Shewanella as a feed additive in the abalone culture. Shewanella (comprising WA64 and WA65 strains) has strong a capability of antagonism of abalone pathogenic bacteria and thus Shewanella can be used as a feed additive for abalone culture. The Shewanella as feed additive for abalone culture has the advantages that Shewanella promotes that juvenile abalones vie with pathogenic bacteria for adhesion sites, maintains a normal microecological balance of the intestinal canal, can colonize in the intestinal canal of an abalone, promotes nutrient absorption, improves immunity thereby improving a growth speed and disease resistance of a Haliotis discus hannai, can replace a chemical drug for disease treatment, and does not damage the ecological environment.

The invention discloses shewanella piezotolerans 34# and an application thereof to algae inhibition. The collection number of the shewanella piezotolerans 34# is CGMCC NO. 6490, and the shewanella piezotolerans 34# has the capacity of producing a long-chain QS (quorum sensing) signal and the function of inhibiting alga. A method provided by the invention has a good effect of inhibiting the red-tide algae, can be used for preventing red-tide organisms effectively, can realize the biological antagonistic prevention and control concept of controlling alga by bacteria and has field popularization value.

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: E. coli W3110, a human related enterobacterium and Shewanella oneidensis MR-1, an environmentally important bacterium with versatile metabolism.

The invention discloses a microbial agent capable of efficiently biologically converting a form of cadmium in polluted soil. The microbial agent can be utilized for efficiently converting cadmium difficult to utilize in the polluted soil into soluble cadmium and mainly contains the following components in percentage by weight: 25%-35% of Candida utilis 15%-20% of Sporotrichum sp., 15%-20% of Acidithiobacillus ferrooxidans, 15%-20% of Sulfobacillus acidophilus and 5%-15% of Shewanella oneidensis. By processing the soil by virtue of the microbial agent, the removal rate of cadmium is 82%-95%. Compared with an existing cadmium polluted soil converting and restoring technique, the microbial agent is capable of efficiently converting the form of cadmium, short in cycle, low in cost and easy to generalize.

The invention discloses shewanella frigidimarina and a method for producing exopolysaccharide. The strain W32-2(shewanella frigidimarina) capable of being used for high-yield production of exopolysaccharide is separated and screened from gravel and sediment samples of the South Pole, and preserved in China General Microbiological Culture Collection Center on Feb 01, 2016 with the preservation number of CGMCC No.12129. A polar region source exopolysaccharide product with antioxidant activity and probiotic activity is prepared by means of the strain W32-2(shewanella frigidimarina), and the product is easy to prepare and has broad market prospects.

The invention belongs to the technical field of biochemical detection, and particularly relates to a bioelectrochemical detection method for vitamin B2. The detection method is characterized by comprising the following steps: forming a reaction liquid system by using an electron acceptor of Shewanella oneidensis, Shewanella oneidensis liquid and a pH buffer solution together; controlling a certain electric potential by adopting a three-electrode system; adding a VB2-containing sample into a reaction liquid of a bioelectrochemical sensor, and detecting the change of a current value; determining the concentration of the VB2 in the sample according to a linear relation between the change of the concentration of the VB2 and a current response value; the detection method does not depend on large-scale instruments and equipment, is low in detection cost, simple in operation and high in accuracy and specificity, and can be applied to detecting the VB2 in biochemical samples, food and medicines.

The invention discloses an immobilized pellet of Shewanella Oneida and the application of Shewanella Oneida in treating printing and dyeing wastewater. The invention proposes for the first time that Shewanella oneida is used in the treatment of printing and dyeing wastewater, and provides a new way for the decolorization of printing and dyeing wastewater. The decolorization efficiency of Oneida Shewanella to printing and dyeing wastewater is particularly outstanding, so the present invention makes Oneida Shewanella into immobilized pellets, and the immobilized pellets not only effectively improve the decolorization efficiency of Oneida Shewanella. Reuse rate, and greatly improve the decolorization efficiency of printing and dyeing wastewater, the highest decolorization efficiency can reach 43.31%.

The invention generally relates to methods, devices, and microbes involved in performing redox imbalanced fermentations. In one aspect, the invention provides a device that generally includes an electrode and at least one microbe in electron communication with the electrode. The microbe generally can exhibit increased activity of at least one enzyme involved in converting a substrate to a redox imbalanced product. In another aspect, the invention provides a method for performing redox imbalanced fermentation. Generally, the method includes providing a substrate to a microbe under conditions effective for the microbe to metabolize the substrate to a redox imbalanced product. At least one microbe may be in contact with an electrode. In some cases, metabolic conversion of the substrate to the redox imbalanced product can include transferring electrons between the electrode and the microbe. In other cases, metabolic conversion of the substrate to the redox imbalanced product exhibits a carbon flux from organic substrate to organic product of at least 80%. In another aspect, the invention provides a genetically modified Shewanella oneidensis microbe.

The invention belongs to the field of bioengineering, and relates to a shewanella putrefaciens phage isolated strain and application thereof. The shewanella putrefaciens phage isolated strain is shewanella putrefaciens phage SppYZU05 with the preservation number being CCTCC NO:M2016717. The invention also discloses bacteria inhibition application of the shewanella putrefaciens phage SppYZU05 as afood additive composition or a biological fresh-keeping agent in food production and storage, particularly, spoilage of fresh water or sea water products in cold chain circulation caused by shewanellaputrefaciens phage multiplication can be inhibited, and the product shelf life is prolonged.

The invention discloses an efficient electron carrier screening, designing and modifying method, and belongs to the field of bioelectrochemical catalysis. According to the invention, Shewanella oneidensis is used as an experimental object to screen and modify an electron carrier so as to improve the extracellular electron transfer capability of the electron carrier. In order to screen out electroncarriers with excellent performance, a single-chamber bioelectrochemical system and a double-chamber bioelectrochemical system are established, a microbial extracellular electron transfer detection method is established and perfected, and evaluation indexes such as cytotoxicity, current density, reduction equivalent and coulombic efficiency are determined. In order to further improve the performance of the electron carrier, the anthraquinone-2-sodium sulfonate (AQS) electron carrier is reasonably designed and modified based on a density functional theory, and seven substituent group positionsand six side chain groups are selected for theoretical calculation of standard Gibbs free energy change and oxidation-reduction potential. Experimental analysis proves that the method for reducing the redox potential of the electron carrier through design and transformation is an effective method for improving the extracellular electron transfer capacity of electroactive microorganisms.

The invention discloses P. denitrificans / S. oneidensis / graphene / calcium alginate denitrifying gel microsphere as well as preparation method and application thereof, and belongs to the technical fieldof environmental protection. According to the invention, calcium alginate is adopted to embed denitrifying microorganisms P. denitrifians, S. oneidensis MR-1 and graphene to form P. denitrifians / S. oneidensis MR-1 / graphene / calcium alginate denitrifying gel microspheres, and the prepared microspheres have strong resistance to toxic and harmful environmental impact, and can realize recycling of strains and materials. When the microsphere is applied to an anaerobic denitrification system polluted by nitrate or co-polluted by nitrate, the time required by reaching 90% of nitrate removal rate can be greatly shortened, and the accumulation of intermediate products nitrite and nitrous oxide is reduced.

The invention discloses a method for rapidly removing hexavalent chromium pollutants by using an electron carrier, and belongs to the technical field of environment. The physiological property that the Shewanella oneidensis MR-1 can be used for removing hexavalent chromium pollutants is used as a basis; a strain is cultured to a stable growth period; thalli are collected and transferred to a reactor to carry out re-suspending; the hexavalent chromium pollutant removal capability of the electron carriers namely riboflavin and anthraquinone-2-sodium sulfonate is measured; under the condition that the concentration of riboflavin is 10-50 [mu]M, the Cr<6+> removal rate of riboflavin is increased to 1.40-2.26 times, the removal rate is increased to 42.0-67.7% from 30.0%, and the Cr<6+> removalrate is higher under the condition that the pH value is equal to 8; anthraquinone-2-sodium sulfonate can also improve the removal speed and removal rate of Cr<6+>, and under a concentration of 100 [mu]M, the maximum removal rate reaches 98.1% and is close to 100%. Hexavalent chromium pollutants can be quickly removed by utilizing the electron carrier, so that the problem of low removal efficiencyof hexavalent chromium pollutants at present is solved, and the method has potential environmental remediation and application values.

The invention discloses a method for repairing chromium-contaminated soil through a biochar-loaded hematite-shewanella loihica complex. Hematite is loaded on biochar by a hydrothermal method, then the hematite and shewanella loihica form a BC@ alpha-Fe2O3 @ loihiica complex, and fulvic acid is added as an electron shuttle, so that the chromium-contaminated soil is repaired. The ratio of the charcoal to the hematite is 10: 1; the proportion of the biochar loaded hematite in the complex is 0.05 g / mL (complex); and the repairing process is carried out under an anaerobic condition, the repairing time is 5 days, and the temperature is 30 + / -2 DEG C. The biochar-loaded hematite-shewanella loihica complex has a good remediation effect on soil polluted by hexavalent chromium with different concentrations, the remediation time is short, the biochar-loaded hematite-shewanella loihica complex can be used for remediation of soil polluted by high-concentration hexavalent chromium, and an efficient and rapid method is provided for clay polluted by high-concentration hexavalent chromium which is difficult to remedy.

The invention relates to the field of pollution management and discloses a method for digesting bottom mud of black and odorous river channel through a microorganism preparation. The microorganism preparation is added into a black and odorous river channel; the microorganism preparation is prepared by mixing CQ1, CQ2 and CQ3 according to the volume ratio of (1 to 3) to (1 to 3) to 1; the CQ1 is amixed bacterium agent of bacillus subtilis, bacillus aureus, acinetobacter, psychrobacter, acinetobacter and pseudomonas strains; the CQ2 is a mixed bacterium agent of arcobacter, acinetobacter and shewanella oneidensis strains; and the CQ3 is a mixed bacterium agent of aeromonas, ralstonia eutropha and ralstonia eutropha strains. By adopting the method disclosed by the invention, the problems that original biological population structures and living conditions of rivers and lakes are damaged in a traditional dredging manner, a self-cleaning function is weakened so that negative influences onecological restoration are caused and secondary pollution is easy to cause, principal and secondary aspects cannot be treated at the same time, people are likely to be disturbed and the management price is high are solved.