7 results about "Shewanella oneidensis" patented technology

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

This invention relates to the field of microbial technology, and more particularly to an alkali-resistant strain of *Shewanella allergensis* and its application in the degradation of ammonia nitrogen in wastewater. The strain is *Shewanella allergensis* DN-SY03, and its classification name is... Alishewanella alkalitolerans DN-SY03, with accession number CCTCC NO: M2026078, is capable of degrading ammonia nitrogen and COD in wastewater. It not only grows normally at low temperatures of 10~25℃ but also achieves highly efficient degradation of ammonia nitrogen and COD in wastewater, providing a new solution for the degradation of ammonia nitrogen and COD in wastewater under low winter temperatures.

The present application relates to the technical fields of synthetic biology and microbial metabolic engineering, and particularly to engineered Saccharomyces cerevisiae for synthesizing beta-carotene by co-utilizing glucose and organic acid and a construction method thereof, wherein the engineered Saccharomyces cerevisiae Sc-Car is used as a starting strain, the GAL80 gene is knocked out, and the SO1522 gene derived from Shewanella oneidensis MR-1 and the ACKA gene derived from Escherichia coli and the PTA gene derived from Clostridium kluyveri are introduced respectively to obtain a recombinant Saccharomyces cerevisiae strain Sc-CarL capable of co-utilizing glucose and lactic acid and a recombinant Saccharomyces cerevisiae strain Sc-CarA capable of co-utilizing glucose and acetic acid; the constructed strain can improve the synthesis capacity of beta-carotene under the condition of mixed carbon source.

This invention relates to a biomodified anode material, comprising: (A) *Pseudomonas putida* YT-1; (B) *Shewanella oneidensis* MR-1; (C) pyrite; (D) sodium alginate-PVA bilayer hydrogel; and (E) a substrate. The above-mentioned anode material of this invention can be used to fabricate microbial fuel cells, wherein the anode material regulates the soil "Fe(III)-Fe(II)" cycle through functional microorganisms, thereby affecting the arsenic speciation in soil pore water, inhibiting the anaerobic reduction reaction of pentavalent arsenic at the anode, promoting the migration of soluble trivalent arsenic in pore water to the cathode and its oxidation to pentavalent arsenic, inhibiting the anaerobic reduction reaction of arsenic in the soil, and promoting the co-precipitation of iron and arsenic to reduce the environmental risk of arsenic pollution.

This application relates to a plant essential oil composition and its application in aquatic product preservation. The plant essential oil composition contains tangerine peel essential oil and bergamot essential oil, which have excellent bactericidal effects against typical dominant spoilage bacteria in aquatic products—Shewanella and Vibrio parahaemolyticus. Furthermore, this invention integrates magnetic induction electric field-assisted extraction and compound essential oil preservation technology, significantly improving the extraction rate and antibacterial activity of plant essential oils. It is the first to construct a synergistic compound formula targeting dominant spoilage bacteria in aquatic products and achieve magnetoelectric-essential oil coupled preservation, extending the refrigerated shelf life of aquatic products by approximately 8 days. It has significant advantages of high efficiency, greenness, and safety, providing new ideas and technical support for the storage and preservation of aquatic products.

This invention proposes an alginate lyase, a compound enzyme preparation, and their applications, belonging to the field of enzyme engineering technology. It solves the technical problem that a single alginate lyase cannot efficiently prepare alginate oligosaccharides directly from brown algae. The alginate lyase of this invention is isolated from Shewanella. Shewanella sp. HD5 The original alginate lyase gene of *Shewanella* was obtained through heterologous expression. Shewanella sp. HD5 The alginate lyase of this invention was deposited on September 22, 2025, at the China Center for Type Culture Collection, Wuhan, Hubei Province, with accession number CCTCC NO: M20252078. This invention provides a high-yield lyase obtained through heterologous expression. It is combined with cellulase, pectinase, and papain to form a complex enzyme preparation that can be directly applied to the enzymatic hydrolysis of brown algae to obtain uniformly polymerized alginate oligosaccharides. Furthermore, the complex enzyme preparation exhibits higher extraction efficiency of alginate oligosaccharides than a single alginate lyase. The prepared alginate oligosaccharides can be used in food, feed, and pharmaceutical fields.