61 results about "Citrobacter" patented technology

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms, such as, Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Escherichia, Salmonella, Bacillus, Streptomyces and Pseudomonas, containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions. Specifically, Citrobacter and, Klebsiella provide the source of exogenous genes for such active dehydratase enzyme.

The invention relates to the fields of genetic engineering and biomedicine, and more specifically relates to a biomarker of schizophrenia and its application. The invention discloses a marker of schizophrenia and its application in preparing a kit for diagnosing schizophrenia, and also discloses a kit for detecting the marker. The schizophrenia markers disclosed in the present invention include Prevotella, Succinivibrio, Fusobacterium, Lactobacillus, and Megasphaera , Acidaminococcus, Citrobacter, Phascolarctobacterium, Coprococcus, Roseburia, Blautia 11 species At least 5 of the microorganisms. The marker provided by the invention can judge schizophrenia and normal people, the AUC is above 0.81, has good diagnostic efficiency, and can be used to prepare a kit for effectively diagnosing schizophrenia.

The invention discloses a microbial herbicide and relates to the technical field of pesticides. The microbial herbicide comprises components in parts by weight as follows: 10-30 parts of cup fungi, 10-20 parts of pseudomonas pseudoacaligenes, 5-10 parts of citrobacter, 3-7 parts of fusarium, 1-5 parts of entyloma, 15-25 parts of sclerotinia sclerotiorum, 3-10 parts of achromobacter and 15-25 parts of halobacterium. The microbial herbicide is prepared from microorganisms such as cup fungi, pseudomonas pseudoacaligenes, citrobacter, fusarium, entyloma, sclerotinia sclerotiorum and the like and has the advantages of high selectivity, safety for non-target organisms and mammal animals, good environment compatibility, few development cost, novel chemical structure and unique action mode by optimizing the proportion of the components.

The present invention provides a novel class of monoclonal antibodies which have a high affinity, broad spectrum neutralizing reactivity to flagellin from various Gram-negative bacteria including, but not limited to, E. coli, Salmonella, Serratia, Proteus, Enterobacter, Citrobacter, Campylobacter and Pseudomonas. The present invention further provides methods of treating inflammatory bowel disease (IBD) and methods of treating enterobacterial infections using anti-flagellin antibodies in humans, other animals and birds.

The invention provides a bioenzyme formaldehyde remover and a preparation method and relates to the field of formaldehyde. The bioenzyme formaldehyde remover comprises, by weight, 5-10 parts of soybean protein, 0.5-2 parts of bioenzymes, 3-5 parts of bacillus subtilis, 1-3 parts of pseudomonas amygdala, 3-5 parts of citrobacter, 1-3 parts of alcaligenes faecalis, 3-5 parts of plant extracts, 1-3 parts of tea polyphenol, 0.5-2 parts of citric acid, 1-2 parts of protective agent and 0.2-1 part of active agent, the bioenzymes include formaldehyde dehydrogenase, polyphenol oxidase, protease and polysaccharide hydrolase, and the plant extracts include aloe extract, ivy extract, maguey extract and dayflower extract. Various microorganisms, the bioenzymes and the plant extracts are adopted to jointly act to remove formaldehyde, so that removal effect is improved; the bioenzyme formaldehyde remover is safe and nonhazardous.

The present invention provides a microorganism for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising a) at least one gene encoding a dehydratase activity; b) at least one gene encoding a glycerol-3-phosphatase; and c) at least one gene encoding protein X. The protein X may be derived from a Klebsiella or Citrobacter gene cluster. The recombinant microorganism may further comprise d) at least one gene encoding a protein having at least 50% similarity to a protein selected from the group consisting of protein 1 (SEQ ID NO:60 or SEQ ID NO:61), of protein 2 (SEQ ID NO:62 or SEQ ID NO:63) and of protein 3 (SEQ ID NO:64 or SEQ ID NO:65) from Klebsiella or Citrobacter.

The present invention is related with the Microbiology field and particularly with a composition and a method for early detection, identification, differentiation and count of microscopic organisms, concretely Gram-negative microorganisms. The composition described in the invention consist on a mixture of substances of protein origin with a total nitrogen content from 9 to 20% and in relationship between 2:1 to 24:1, concerning to the content of inhibitors of the Gram-positive organisms. It contains a mixture of organic and inorganic substances that facilitate the differentiation of the Gram-negative organisms, being this mixture in a relationship from 0.5:1 to 2:1 concerning to the mixture of substances of protein origin. The referred composition allows the detection and differentiated count of E. coli and other coliform organisms due to the blue-greenish color of the colonies of these microorganisms on the orange bottom of the medium; Salmonella not typhi for the red color of the centers of the colonies on rosy bottom of the medium; Salmonella typhi and Proteus for the transparency of the colonies; Citrobacter and Klebsiella for the violet color of the colonies on the pink to orange bottom of the medium and Pseudomonas aeruginosa for the orange color with darker center of the colony, taking greenish pigmentation after 24 hours and producing greenish fluorescence under low ultraviolet light.

The invention relates to a method for purifying tetravalent selenium-containing sewage under an anaerobic condition and an aerobic condition by utilizing citrobacter freundii. According to the method, the Citrobacter freundii Strain Y9, bought from America Type Culture Collection, is taken as a strain, the citrobacter freundii is inoculated in the tetravalent selenium-containing sewage under the anaerobic condition and the aerobic condition and takes sodium citrate, sodium acetate or sodium nitrate as an electron donor, tetravalent selenium in the wastewater is taken as an electron acceptor, and the tetravalent selenium is reduced into element selenium by the Citrobacter freundii Strain Y9 after a biochemical treatment, so that the aim of removing the tetravalent selenium from the sewage under the anaerobic condition and the aerobic condition is realized. The method is simple in process, convenient to operate, low in treatment cost, large in treatment range and free of secondary pollution.

The invention belongs to the technical field of microbial degradation, and specifically relates to a compound microbial agent for kitchen oil and wastewater treatment, and a preparation method and applications of the compound microbial agent. In order to reduce the pollution of oil pollutants to environment, the compound microbial agent for kitchen oil and wastewater treatment is provided. The compound microbial agent includes Bacillus, Citrobacter and Pseudomonas, and the effective viable count ratio of each bacterium is 2-10: 1-4: 3-9. The preparation method of the compound microbial agent and applications of the compound microbial agent in oil degradation are also provided. Degradation can be performed by inoculating the compound microbial agent in oily wastewater or activated sludge during using according to the inoculating amount of 1.0-15%. The compound microbial agent is suitable for the treatment of kitchen wastewater, can reduce odor emission and can be industrially applied ona large scale; and the compound microbial agent is small in investment and high in efficiency and has no secondary pollution.

The invention discloses a lake riverway ecological restoration agent. The lake riverway ecological restoration agent is prepared from, by weight, 60-80 parts of nitrifying bacteria, 20-30 parts of mycobacterium szulgai, 20-30 parts of bifidobacteria, 16-20 parts of yeast, 6-8 parts of neutral citrobacter, 6-8 parts of bacillus subtilis, 6-8 parts of Yulei bacteria, 6-8 parts of actinomycetes, 6-8parts of thiobacillus, 6-8 parts of thermophilic desmolysing bacteria, 6-8 parts of bacillus cereus, 6-8 parts of bacillus flexus, 6-8 parts of paenibacillus, 6-8 parts of brevibacillus choshinensis 6-8 parts of saccharomyces cerevisiae, 6-8 parts of corn flour, 6-8 parts of soybean meal, 16-20 parts of oxidant, 10-12 parts of flocculant and 40-60 parts of carriers. According to the lake riverwayecological restoration agent, through the aerobic and anaerobic respiration of the nitrifying bacteria, mycobacterium szulgai, bifidobacteria, yeast, neutral citrobacter, bacillus subtilis, actinomycetes, thiobacillus, thermophilic desmolysing bacteria, bacillus cereus, bacillus flexus, paenibacillus, brevibacillus choshinensis and saccharomyces cerevisiae, organic and inorganic substances in water can be decomposed and absorbed, and the eutrophication root of the water is cut off.

The invention discloses a soil ecological restoration material and a preparation method thereof. The soil ecological restoration material is prepared from the following components in parts by weight: 6 to 12 parts of Nocardia globerula, 4 to 9 parts of bacteria nitroxide, 15 to 25 parts of lactic acid bacteria, 8 to 16 parts of bacteria nitroxide, 7 to 18 parts of neutral citrobacter, 3 to 15 parts of white rot fungi, 4 to 9 parts of Yulei bacteria, 1 to 7 parts of actinomycetes, 1 to 5 parts of aspergillus, 3 to 9 parts of bacillus natto, 4 to 11 parts of bacillus licheniformis, 3 to 11 parts of bacillus cereus, 4 to 13 parts of rhodopuesdomonas pulstris, 40 to 100 parts of animal manure, 30 to 90 parts of plant straw and 30 to 80 parts of water. The invention further discloses a preparation method for the soil ecological restoration material. The preparation method comprises the following specific steps: producing seeds; uniformly stirring, and placing the seeds into a fermentation room for performing anaerobic fermentation for 2 to 4 weeks. The soil ecological restoration material has the advantages of good soil restoration effect, and environmental friendliness and the like.

The invention discloses an enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof, and belongs to the technical field of agricultural microorganisms. The invention discloses an enhanced high-efficiency nitrogen-fixing bacteria, which comprises at least one of klebsiella MNAZ1050, citrobacter MNAZ1397 and pseudomonas MNAZ228; the invention further discloses an enhanced high-efficiency nitrogen-fixing complex microbial system which comprises nitrogen-fixing bacteria and non-nitrogen-fixing bacteria; wherein the nitrogen-fixing bacteria comprise at least one of the above three bacteria; the non-nitrogen-fixing bacteria comprises at least one of acinetobacter ACZLY512 and kluyveromyces AZ981. The nitrogen-fixing bacteria in the nitrogen-fixing complex microbial system have a better nitrogen-fixing effect when being independently used, and the nitrogen-fixing effect of the nitrogen-fixing complex microbial system constructed by cooperatively using the nitrogen-fixing bacteria and the non-nitrogen-fixing bacteria is more remarkable than that of the nitrogen-fixing bacteria when being independently used, so that crop growth can be promoted under the condition of obviously reducing nitrogen fertilizer application, and the nitrogen-fixing complex microbial system has important significance for improving the crop yield.

The invention discloses citrobacter vinegaticus and application of the citrobacter vinegaticus in removal of chloramphenicol and dissolution of phosphorus and potassium. The name of the citrobacter vinegatus is Citrobacter portucalensis SZW2, the preservation number of the citrobacter vinegatus is GDMCC NO: 61695, and the citrobacter vinegatus is preserved in Guangdong Province Microbial Culture Collection Center on the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City on May 27, 2021. The Citrobacter portucalensis SZW2 can remove chloramphenicol in a co-metabolism mode and can secrete organic acid substances to reduce the pH value in a system, so insoluble phosphorus and potassium substances are dissolved, the content of available phosphorus and potassium in soil can be increased, and therefore, the Citrobacter portucalensis SZW2 can be used for removing chloramphenicol or promoting plant growth.

The invention discloses low-molecular-weight alfalfa polysaccharide as well as a preparation method and application thereof in regulating intestinal flora, the molecular weight of the low-molecular-weight alfalfa polysaccharide is 3-6 KDa, and monosaccharide comprises 0.75-1.16% of mannose, 68.57-81.02% of glucose and 1.10-4.53% of galactose; The low-molecular-weight alfalfa polysaccharide is prepared by cellulase extraction, Sevage reagent deproteinization, cotton-wool-shaped DEAE-52 cellulose decoloration and granular DEAE-52 cellulose separation and purification. The low-molecular-weight alfalfa polysaccharide has the effects of regulating intestinal flora, promoting the growth of probiotics such as bifidobacterium and lactobacillus, inhibiting the growth of pathogenic bacteria such as citrobacter and escherichia coli, generating short-chain fatty acids such as acetic acid through glycolysis, and reducing the pH value of the intestinal tract.

The invention belongs to the technical field of microbial degradation, and particularly relates to a grease degradation complex microbial inoculant as well as a preparation method and an application thereof. In order to reduce the pollution of grease pollutants to the environment, the invention provides the grease degradation complex microbial inoculant which comprises bacillus, Klebsiella and Citrobacter, and the effective viable count ratio of the bacillus to the Klebsiella to the Citrobacter is (2-8): (1-6): (1-4). The invention also provides the preparation method of the grease degradationcomplex microbial inoculant and the application of the grease degradation complex microbial inoculant in grease degradation. When in use, the strain is inoculated into oily wastewater or activated sludge according to an inoculation amount of 1.0-15% for degradation. The complex microbial inoculant is suitable for treating grease wastewater with various concentrations, and is also suitable for various sewage treatment technologies such as aerobic, facultative, anaerobic and the like. The complex microbial inoculant can be industrially applied on a large scale, and has the advantages of small investment, high efficiency, no secondary pollution and the like.

The invention discloses a method for repairing uranium-cadmium combined polluted soil with microorganisms, and aims to solve the problems that an existing heavy metal soil repairing method mainly aimsto repair soil polluted by a single heavy metal, and no effective repairing measure is provided for soil polluted by heavy metals cadmium and uranium at the same time. The method for repairing the uranium-cadmium combined polluted soil with microorganisms comprises the following steps: adopting a compound bacterial strain in repairing of the uranium-cadmium combined polluted soil, wherein the compound bacterial strain is formed by compounding bacillus subtilis, citrobacter and bacillus cereus. The three bacterial strains are compounded to obtain the microbial compound at a specific ratio, sothat the removal level of metal uranium and metal cadmium in soil can be greatly improved, and the microbial compound has a good removal effect. The method is simple to operate, good in removal effecton metallic uranium and metallic cadmium, high in removal rate and excellent in soil remediation effect.

The invention discloses an environment-friendly deodorization device and method for kitchen wastewater. The environment-friendly deodorization device comprises a deodorization tank, a microbial agentmixing unit and a tank body heating unit, a feeding channel communicating with the interior of the deodorization tank is obliquely arranged on the side face of the top of the deodorization tank upwards, the microbial agent mixing unit is installed on the feeding channel, and a mixed microbial agent used for degrading starch, protein, grease and cellulose is contained in a microbial agent containing box of the microbial agent mixing unit. The mixed microbial agent is prepared from dried bacillus, citrobacter and lactobacillus. Main components including starch, protein, grease and cellulose in the kitchen wastewater are degraded by using the mixed microbial agent of bacillus, citrobacter and lactobacillus. A mechanical temperature control module can effectively control a proper temperature,so that the mixed microbial agent is promoted to degrade two odors including ammonia gas and hydrogen sulfide at the same time at the proper temperature, and odor generation can be reduced, and odor can also be degraded.

The invention discloses a microbial flora for preventing and treating wheat scab. The microbial flora mainly comprises two major components, namely bacteria and actinomycetes, wherein the identified bacterial components also comprise bacillus, pseudomonas, pantoea, bifidobacterium, bacillus aceticus, lactobacillus, stenotrophomonas, klebsiella, citrobacter and flavobacterium. The microbial flora has a relatively strong prevention and control effect on wheat scab and gibberellin thereof; the microbial flora is wide in raw material source, low in production and preparation cost, quick in effectin field application and free of secondary pollution to the environment; and the microbial flora disclosed by the invention is separated from infected straws, and is relatively strong in adaptabilityto a complex repair environment in the field and relatively significant in repair effect.

The invention discloses a preparation method of dye decolorizing complex microbial inoculant. The method comprises the following steps: separating mangrove sea mud to obtain Citrobacter H-3, Novosphingobium TH-5 and Paenibacillus TH-7 with relatively strong dye decolorizing performance, and respectively culturing to obtain bacterial liquids; and mixing the bacterial liquids, culturing and domesticating to obtain the complex microbial inoculant. The complex microbial inoculant has excellent decolorizing and degrading performance on simulated dyeing wastewater, is simple in preparation method and low in cost, can widely grow in a pH and saline environment, and has decolorizing and degrading performance on various dyes. Compared with single bacterium, the complex microbial inoculant has the advantage that the decolorizing effect is remarkably improved. Due to synergistic combination of Citrobacter, Novosphingobium and Paenibacillus, the dye in the dyeing wastewater can be quickly decolorized and degraded by the complex microbial inoculant, and secondary pollution cannot be caused.

The invention discloses an application method of bacillus subtilis in indoor formaldehyde removal. The application method comprises the following steps: preparing a bio-enzyme formaldehyde scavenger from bacillus subtilis and other raw materials, activating bacillus subtilis and citrobacter to prepare a bacillus subtilis bacterial solution and a citrobacter bacterial solution, uniformly mixing thebacillus subtilis liquid, the citrobacter liquid and other raw materials to obtain the bio-enzyme formaldehyde scavenger, detecting a formaldehyde content value in the indoor environment, and directly spraying a bio-enzyme formaldehyde scavenger to the indoor and indoor furniture surfaces in response to the fact that the content value is greater than a preset value, or directly adding the bio-enzyme scavenger into a decoration material during house decoration. According to the bio-enzyme formaldehyde scavenger prepared from bacillus subtilis and other raw materials, formaldehyde is removed through the combined action of bacillus subtilis, other microorganisms, bio-enzyme and plant extracts, the removal effect is effectively improved, and the bio-enzyme formaldehyde scavenger is safe, harmless, biodegradable and free of secondary pollution to the environment.