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Microorganism bacterium agent for processing thick oil sewage and preparation method thereof

A microbial agent and heavy oil sewage technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of heavy oil sewage that cannot meet the discharge standards, and avoid incomplete sewage treatment, The effect is obvious and easy to operate

Inactive Publication Date: 2007-07-04
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a compound microbial agent for treating heavy oily sewage and its preparation method. The microbial agent has a strong synergistic effect, can improve the biodegradability of heavy oily sewage in the treatment of heavy oily sewage, and can more effectively degrade the heavy oily sewage. The refractory organic pollutants in the oily sewage can well solve the problem that the heavy oily sewage cannot be discharged up to the standard

Method used

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  • Microorganism bacterium agent for processing thick oil sewage and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0022] This embodiment adopts self-prepared oily waste water as (gL -1 ): Crude oil 10g, ammonium sulfate 0.5g, sodium nitrate 0.5g, calcium chloride 0.02g, magnesium sulfate 0.2g, potassium dihydrogen phosphate 1.0g, sodium dihydrogen phosphate 1.0g, adjust pH 7.5, degrade petroleum hydrocarbons indoors experiment.

[0023] The bacterial agent adopts bacterial strains, specifically: 10% Pseudomonas aeruginosa, 15% Bacillus subtilis, 8% Bacillus licheniformis, 12% Propionibacterium freundii freudennreichii), 14% Aureobacterium liquefaciens, 5% Bacilluscirculans, 10% Curtobacterium flaccumfaciens, 10% Arthrobacter globiformis, 6% Clavibacter xyli) and 10% Anaerobaculum thermoterrenum

[0024] The specific operation steps of the preparation method of microbial bacterial agent are as follows:

[0025] 1) Activation of strains: using the strains, inoculate the strains on the slant culture medium in the prepared test tube (in this example, bacterial culture medium) by convention...

Embodiment 2

[0036] The difference from Example 1 is:

[0037] The water used in the experiment is heavy oil production water from Liaohe Oilfield, which has complex components. Among them, the organic pollutants are mainly alkanes, aromatics, organic acids and petroleum colloids, and the content of unsaturated hydrocarbons and non-hydrocarbons is relatively low. The proportion of substances below 12 carbons in saturated hydrocarbons is not high, and the proportion of high-carbon chains and aromatic substances is relatively high. The sewage is difficult to degrade, and the average value of CODcr is about 300mg / L.

[0038] The bacterial agent adopts bacterial strains, specifically: 8% Pseudomonas aeruginosa, 12% Bacillus subtilis, 5% Bacillus licheniformis, 10% Propionibacterium freundii freudennreichii), 20% Aureobacterium liquefaciens, 10% Bacilluscirculans, 5% Curtobacterium flaccumfaciens, 5% Arthrobacter globiformis, 10% Clavibacter xyli) and 15% Anaerobaculum thermoterrenum.

[0039...

Embodiment 3

[0051] The difference from Example 1 is:

[0052] The experimental water is heavy oil sewage from Liaohe Oilfield Wastewater Treatment Plant. The water quality of the sewage is alkaline. After oil separation and air flotation treatment, the CODcr concentration is 200-220mgL -1 , BOD5 value is very low, BOD5 / CODcr is less than 0.10, basically no biodegradability. Petroleum and organic polymers such as emulsifiers and demulsifiers are the main pollutants, and they are also the main refractory pollutants.

[0053] The bacterial agent adopts bacterial strains, specifically: 5% Pseudomonas aeruginosa, 10% Bacillus subtilis, 15% Bacillus lichenformis, 15% Propionibacterium freundii ( Propionibacterium freudennreichii), 10% Aureobacterium liquefaciens, 9% Bacillus circulans, 9% Curtobacterium flaccumfaciens, 9% Arthrobacter globiformis, 5% Clavibacter xyli) and 13% Anaerobaculum thermoterrenum.

[0054] The specific operation steps of the preparation method of microbial bacterial ...

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Abstract

The invention relates to the biological treatment for oil extraction wastewater, which in detail relates to a bacterial agent for treating thick oil wastewater and the method for preparing the same. The comprised components and their proportion by weight are as follows: pseudomonas aeruginosa 5-10%, bacillus subtilis 10-15%, lichen bacilli 5- 15%, Citrobacter propionate tumefaciens 10- 15%, liquid gold tumefaciens 10- 20%, annular gemma bacillus 5- 10%, wilting Bacillus pumilus 5- 10%, spherical arthrobacter 5- 10%, crabstick tumefaciens 5- 10%, and hot ground anaerobic rod baceria 10- 15%. It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing. The cooperation action is strong, the biological surface activating agent generating- bacteria can enlarge the dissolution of hydrocarbon petroleum in water and make it is easier for hydrocarbon petroleum to contact with bacteria; the anaerobic bacteria can improve the biodegradation of thick oil wastewater; the degradation perfomace of aerobic bacteria is good and suitable for biological treatment for thick oil wastewater.

Description

technical field [0001] The invention relates to biological treatment technology of oil production sewage, in particular to a composite microbial bacterial agent for treating heavy oil sewage and a preparation method thereof. Background technique [0002] With the development of the petroleum industry, most oilfields in my country have entered the stage of thermal recovery of heavy oil with high water content or super heavy oil, and the water content in crude oil production fluid is as high as 80% to 90%. There have been many reports at home and abroad on the treatment of oilfield produced water. Most of the treatment of heavy oil sewage at home and abroad is reused in the boiler as circulating water after advanced treatment. The treatment of heavy oil sewage generally includes oil-water separation, coagulation sedimentation, air flotation and other processes. The biggest drawback is that it cannot meet the standard discharge (mainly is COD up to standard). After advanced t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/385C12R1/125C12R1/10C12R1/09C12R1/06
CPCC12N1/20C02F3/341C02F3/343C12N1/26C12P39/00
Inventor 郭书海李凤梅王鑫牛明芬
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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