153results about How to "Low biological toxicity" patented technology

The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.

The invention relates to a medical catheter and a method for forming an antibacterial hydrophilic coating on the surface of the medical catheter. The method includes the following steps of preparing graphene oxide dispersoid, dissolving hydrophilic polymers and cellulose ester in organic solvent to form a hydrophilic macromolecular solution, adding the hydrophilic macromolecular solution to the graphene oxide dispersoid in the stirring process to form an antibacterial hydrophilic macromolecular solution, soaking the catheter in a coupling agent solution after conducting surface processing on the catheter so that the surface of the catheter can be dip-coated with a coupling agent layer, soaking the catheter coated with the coupling agent layer in the antibacterial hydrophilic macromolecular solution, and preparing the medical catheter with the antibacterial hydrophilic coating after the catheter is taken out, dried and solidified. Due to the fact that the antibacterial hydrophilic coating is formed on the surface of the catheter, the coating contains graphene oxide, other hydrophilic macromolecules and other components, the catheter can have excellent antibacterial and bacteriostatic capacity, the contact angle of the catheter and water is reduced, and the surface of the catheter can have the ultra-strong lubrication effect and the bacteriostatic effect at the same time.

The invention relates to a preparation method of transition metal doped carbon fluorescent quantum dots. According to the method, a metal chelator and transition metal salt are dissolved in an organicphase and water which are incompatible with each other, then, a heating reaction is conducted, concentration and purification are performed after the reaction, and transition metal doped carbon fluorescent quantum dots with different solubility are prepared. The method is convenient to operate, doping of carbon fluorescent quantum dots with metal ions can be realized without harsh reaction conditions or large instruments. The obtained carbon dots have multiple different characteristics according to different types of doping metal ions and different solvents. The prepared carbon dots have great application value in preparation of bio-labeling sensing and medical imaging, photoelectric and light-emitting devices and the like due to these characteristics.

The invention discloses a method for treating the mixed waste water of the electroplating industry, which comprises the following steps of: firstly, reacting ferrous salt and the mixed waste water of the electroplating industry, then adding sodium hydroxide or lime, regulating the pH value to 6.5 to 7.5 and precipitating for 1.5 to 2h, wherein the concentration of the ferrous salt in the mixed waste water of the electroplating industry is 2000 to 4000mg/L; after precipitating the waste water, carrying out aerobic biochemical treatment on the obtained supernatant liquid by an aerobic biochemical treatment system filled with ceramsite with the detention time of 4 to 8h and removing the COD value and the residual cyanogen in the mixed waste water; and adding a chemical oxidant of sodium hypochlorite into the treated waste water, further oxidizing the cyanogen in the waste water, regulating the pH value of the waste water to 7 to 8.5 and precipitating again for 1.5 to 2h. The method has relatively low treatment cost, not only heavy metal and cyanide reach the standard, but also the COD value in the waste water can be obviously lowered, and the treated waste water reaches a novel discharge standard.

The invention belongs to the technical fields of preparation and application of metal optical function nano materials and in particular relates to a fluorescent silver nanocluster as well as a simple preparation method and application thereof. The fluorescent silver nanocluster is synthesized by reacting soluble silver salt with a stabilizer, wherein the fluorescent silver nanocluster has the mean particle size of more than 1.5nm and less than 2nm; the fluorescence emitted by the fluorescent silver nanocluster has the wavelength of 610nm under excitation of light with the wavelength of 510nm; the emitted fluorescence has high illumination stability. The method is readily available in raw materials, simple in process, convenient to operate, low in biotoxicity and easy to popularize and apply. The synthesized fluorescent silver nanocluster can be applied to the fields of optical imaging, biological markers, biological or chemical sensors and the like, and especially can be used for qualitative and quantitative detection on the activity of alpha-L-fucosidase.

The invention provides a method for inhibiting peroxidase activity by using fluorescent graphene quantum dots. The method comprises the following steps: (1) adding a carbon source into a certain amount of water to prepare a mixed solution, heating the solution until the moisture is completely evaporated, transferring the solution into a high pressure reactor, heating and reacting for a period of time, dissolving the product in water, regulating the pH value of the system to be neutral by using alkali liquor, purifying the product, and drying, thereby obtaining solid graphene quantum dots; and (2) dispersing the quantum dots obtained in the step (1) in secondary distilled water, taking a proper amount of quantum dot solution and enzyme solution, adding the solution into a buffer solution, adding a color developing agent and H2O2 after a period of time, uniformly shaking the mixed solution, rapidly adding the solution into a cuvette, measuring the light absorption value of the maximum absorption wavelength of the product on an ultraviolet-visible spectrophotometer, and performing blank sample control, thereby determining the relative enzyme activity. The graphene quantum dots are prepared by adopting low-price and readily available raw materials, and the method is green and environment-friendly. The structure of the enzyme can be changed by virtue of the interaction between the graphene quantum dots and zymoprotein, and regulation of the enzyme activity is realized.

The invention discloses a recycling treatment system of high-salinity high-organic-matter chemical wastewater. The recycling treatment system comprises an electrocatalytic oxidation device, a halotolerant bacteria biochemical system, an MBR aerobic device, an electroosmosis unit, an ultrafilter and an RO unit, the halotolerant bacteria biochemical system comprises a halotolerant bacteria anaerobic device, a halotolerant bacteria aerobic device and a halotolerant biological aerated filter. The invention further discloses a treatment method of the high-salinity high-organic-matter chemical wastewater. By the recycling treatment system and the treatment method, the problem of recycling treatment of the high-salinity high-organic-matter chemical wastewater is solved effectively, recycling rate of water and salt is high, no wastewater is discharged basically, no solid waste is generated, generated water is high in quality, generated salt is high in purity, and water resources and salt in the high-salinity high-organic-matter chemical wastewater are recycled to greatest extent; zero discharging of wastewater in the process of enterprise production is realized, and the recycling treatment system and the treatment method have important environment benefit.

The invention relates to a catalyst for removing nitrogen oxide through selective catalytic reduction for an automobile diesel engine adopting Ce2O3 and V2O5 double active compositions. The catalyst adopts scordierite honeycomb ceramics as a framework material, the Ce2O3 and the V2O5 as main active compositions, WO3 or MoO3 as an auxiliary catalyst composition, and TiO2 as a coating substrate, wherein the mass fraction of the main active compositions, namely the Ce2O3 and the V2O5, to the auxiliary catalyst composition WO3 or MoO3 to the coating substrate TiO2 is 1-3:6:12:85-93. The specific process comprises the following steps: preparing a TiO2 precursor solution; coating a TiO2 coating; coating an auxiliary catalyst; determining the loading of active compositions of oxides; and coating the main active compositions, namely the Ce2O3 and the V2O5. The catalyst replaces the V2O5 with high toxicity with the Ce2O3 with low toxicity, and reduces the environmental hazards of the catalyst, and the replaced catalyst has wider active temperature range, particularly greatly improves the low-temperature activity, and is particularly suitable for the traffic conditions of urban highways of China.

The invention discloses a method for PMIDA wastewater produced by using an IDA method. The method comprises the following steps: regulating pH of wastewater of PMIDA mother liquid condensate to be 12 by using quick lime and liquid caustic soda; introducing steam and heating to be at 50-60 DEG C; performing alkali condensation and introducing into a biological water distributing tank; regulating pH of secondary washing water to be 1-3 and performing a micro-electrolytic reaction; adding hydrogen peroxide and performing catalytic oxidization in the presence of a surface catalyst; regulating pH to be 7.5-8; pumping wastewater into a biochemical system; using a strain which can be tolerant to 1-3 percent of salinity; degrading organic pollutants by using an ABR anaerobic I/continuous aerobic I/ABR II/continuous aerobic II method and simultaneously turning organophosphorus into orthophosphate; precipitating sludge and transporting outwards; enabling liquid supernatant to flow into a phosphorous removal reaction tank; adding coagulants and lime to be fully subjected to reaction to enable mixed liquid to flow into a phosphorous removal precipitation tank; enabling the liquid supernatant to reach the discharge standard; transporting outwards the sludge. The method is low in operation cost; effluent water is subjected to biochemical treatment so that COD completely reaches the discharge standard; after chemical phosphorous removal, the total phosphorous reaches the discharge standard, so that the pollutants are reduced to the lowest level, the chromaticity is greatly reduced, and the method has a good environmental effect.

The invention relates to a dendritic multi-amino polymer drilling fluid used for mud rock well drilling. The drilling fluid is characterized by consisting of a dendritic multi-amino polymer, drilling fluid bentonite, sodium carbonate, a polymer coating agent, potassium polyacrylate, a hydrolyzed polyacrylonitrile ammonium, a bit balling resistant lubricant and water. The dendritic multi-amino polymer drilling fluid can improve an inhibitive performance of the drilling fluid, can greatly improve the inhibitive performance on clay and drilled chips of the drilling fluid, can reduce plasticity of daub rock, can avoid water-absorption swelling and dispersing of the daub rock, can reduce friction resistance, can prevent bit balling, can increase a penetration rate and can ensure safety of a downhole operation.

The invention relates to a synthetic method of admium telluride/cadmium sulfide/zinc sulfide quantum dots. An inner core is an admium telluride quantum dot and is coated by cadmium sulfide and zinc sulfide in sequence. The synthetic method for the quantum dots comprises the following steps of: firstly, preparing an admium telluride quantum dot with excellent fluorescent characteristic by hydrolyzing a sulfhydryl group; secondly, adding thiourea with a certain concentration into the reacted admium telluride solution, forming free suplfur ions released by slowly hydrolyzing and photolyzing thiourea and cadmium ion dangling bonds existing on the surface of the admium telluride quantum dot into bonds; generating a thin cadmium sulfide casing layer on the surface of the cadmium sulfide quantum dot; and finally adding a zinc acetate solution and a sodium sulfide solution into the admium telluride/cadmium sulfide quantum dots with nuclear shell structures and carrying out hydro-thermal growth in a polytetrafluoroethylene digestion tank to form the admium telluride/cadmium sulfide/zinc sulfide quantum dots. The quantum dots have the characteristics of high fluorescent strength, favorable stability, high quantum yield and low biotoxicity and are hopeful to be applied to the field of biomarkers instead of the traditional organic dye. The synthetic method has the advantages of simple process, convenience, low cost and strong operability.

The invention discloses a fluorescent probe and a manufacturing method thereof and application of the fluorescent probe in detection on ribose nucleic acid (RNA). The probe has a thiazole orange styrene structure which is shown in a chemical structural formula (I) (please see the formula in the description) and is simple and stable in structure and easy to manufacture. The probe can be used for specific detection on RNA, wherein RNA in a solution can be quickly detected through a fluorespectro photometer or directly through visual inspection under fluorescent lamp irradiation; the probe also can be used for detecting or labeling or displaying existence and distribution of RNA in living cells. The fluorescent material has the efficient and specific recognition capacity on ribose nucleic acid (RNA), has the advantages of very good cell membrane permeability, low photoinduced toxicity, biotoxicity and light bleaching property and the like and overcomes the defects that other detection methods are high in cost and equipment requirement, relatively complex in technical operation and the like.

The invention relates to chitosan hydrogel dressing and a preparation method thereof, and belongs to the technical field of hydrogel dressing in a biological medical material. The chitosan hydrogel dressing is prepared from 1 to 8 parts of chitosan or a derivative thereof, 0.1 to 3 parts of gamma-polyglutamic acid or salt thereof and 6 to 10 parts of oxidized hyaluronic acid or salt thereof. The chitosan hydrogel dressing provided by the invention can be prepared by taking water as a solvent and the biological cross-linking agent oxidized hyaluronic acid as a cross-linking agent through Schiffbase reaction, without the need of introducing other cross-linking agents and initiators; the cost and the biological toxicity of a gel product are extremely reduced, and the safety of clinical application of a gel material is ensured. The dressing has excellent biocompatibility and water absorbability; the cross-linking agents and the initiators are not added, so that the chitosan hydrogel dressing is safe and has no toxin, and a production process and a degraded product of the dressing do not pollute the environment.

The invention relates to a near infrared fluorescence probe adopting nile blue as a parent, a preparation method thereof and applications thereof. The fluorescence probe has a structure shown as the general formula I as the attached drawing 1. The fluorescence probe can effectively improve deficiencies of tumor labeling fluorescence probes at present, can sensitively and accurately response to target cells COX-2 expression amount of which is abnormal, and is suitable for effective specific near infrared fluorescence probes labeling living tumor cells. The fluorescence probe provided by the invention is simple in synthesis and products are easy to obtain. The fluorescence probe provided by the invention has a very low fluorescence background in non-tumor cells and high fluorescence signals in tumor cells, and has strong specific labeling function for tumor cells. In addition, compounds of the type have good cell membrane permeability, low biotoxicity, low phototoxicity and low photobleaching capability and can locate a special organelle of a certain kind tumor cell.

The invention discloses a preparation method of a water-soluble bi-colour fluorinated graphene quantum dot. The preparation method of the water-soluble bi-colour fluorinated graphene quantum dot is characterized in that graphite fluoride is taken as a raw material, and stripping, modification and refluxing are carried out, so that a blue and yellow fluorescent fluorinated graphene quantum dot with good water solubility and stable chemical properties is obtained. The obtained water-soluble bi-colour fluorinated graphene quantum dot has great application prospects in the aspects of biology and sensing as well as photovoltaic devices, and is a novel fluorescent nano material with a high application value.

The invention relates to an adhesive for carrying out heat vulcanization adhesion on EPDM (Ethylene-Propylene-Diene Monomer) and metal. The adhesive is prepared from the following components in parts by weight: 100 parts of chlorosulfonated polyethylene rubber CSM, 50 to 70 parts of p-dinitrosobenzene, 50 to 70 parts of polyisocyanate PK preparation which is subjected to end sealing by a silane coupling agent KH 550, 30 to 40 parts of packing and 960 to 1080 parts of mixed solvent, wherein the mixed solvent is a blend solvent of tetrachloroethylene and xylene, and the mass ratio of the tetrachloroethylene and the xylene is 1:1. The adhesive for carrying out the heat vulcanization adhesion on the EPDM and the metal, provided by the invention, is stable in storage performance and simple in using technology and has a single-coating system; the adhesive has higher adhesion strength on adhesion of the EPDM of a peroxide vulcanization system and a sulfur vulcanization system and the metal, the adhesion strength after vulcanization is greater than or equal to 3 MPa, and the peeling strength is greater than or equal to 10 KN/m under 180 DEG C.

The invention belongs to the field of nanomaterials and medical engineering and relates to antioxidant Ce-doped CQDs (carbon quantum dots) as well as a preparation method and an application thereof. Glycine is taken as a surface passivator, citric acid is taken as a carbon source material, cerium nitrate provides a cerium element, residues and water are removed through dialysis and freeze drying with a one-step hydrothermal carbonization method, and the Ce-doped CQDs are obtained. The prepared Ce-doped CQDs have excellent characteristics of a higher fluorescence property, better antioxidant effects and good biocompatibility and the like, are successfully applied to antioxidant experiments of in-vitro live cells and have broad application prospects in the field of biomedicine. The providedpreparation method has the characteristics of being simple in process, low in cost and prone to mass production.

The invention belongs to the soil environment management field in the environmental engineering and relates to the construction and application of a new genetically engineered microorganism capable of restoring the soil polluted by heavy metal cadmium. The preparation method of the genetically engineered microorganism comprises the following steps: monkey metallothionein alpha structure domain tetramer is fused in the ice-nucleating protein N-end of Xanthomona campestris ATCC33913 to be used as a main functional unit for constructing a broad host range expression plasmid; then cadmium-specific promoter is used for expression in Pseudomonas putida X4 which can resist various heavy metals efficiently, thus realizing the surface display of monkey metallothionein alpha structure domain tetramer in the bacterium and obtaining genetically engineered microorganism CH01 which can adsorb heavy metal cadmium efficiently and specifically. The engineered microorganism can remarkably adsorb heavy metal cadmium in the presence of various heavy metals; and the result of the symbiotic water culture with wheat, cowpea and corn shows that the engineered microorganism CH01 can colonize in the rhizosphere of plant and fast adsorb cadmium ions. The invention also discloses a construction method of the genetically engineered microorganism CH01 of which preservation number is CGMCC No.M209320.

The invention discloses a core-shell type nano-contrast agent. The core-shell type nano-contrast agent comprises gadolinium ions and human serum albumin assembled outside the gadolinium ions and serving as a protecting group. The invention further provides a preparation method of the core-shell type nano-contrast agent. The core-shell type nano-contrast agent disclosed by the invention is combined with a nano-technology, and the human serum albumin is taken as a carrier for wrapping the paramagnetic metal gadolinium ions, thus MRI (magnetic resonance imaging) is realized, and biocompatibility and a tumor targeting property are improved; the prepared core-shell type nano-contrast agent has the advantages of small particle size and low biotoxicity; and the preparation method provided by the invention is simple.

The invention aims to provide a wastewater treatment matter, and discloses a preparation method of a water-treatment iron-carbon micro-electrolysis agent. The preparation method of the water-treatment iron-carbon micro-electrolysis agent comprises the following steps: mixing 4L of scrap iron sieved by a 100 mesh sieve, 4L of powder activated carbon and 2L of bentonite, adding water and ammonium bicarbonate, stirring evenly, making into balls by artificial granulating, then drying for 20min at 105 DEG C, and roasting at high temperature. The water-treatment iron-carbon micro-electrolysis agent can effectively remove organic matters in the wastewater, reduce biotoxicity of the wastewater and improve the biodegradability of the wastewater.

The invention discloses a porous chitosan microcarrier as well as a preparation method and an application thereof. The porous chitosan microcarrier takes chitosan as a main component, the diameter ofthe porous chitosan microcarrier is 100-300 mu m, interconnected pores are formed in the surface and the interior of the porous chitosan microcarrier, the diameter of the pores is 10-70 mu m, and theporosity is 95% or higher. The porous chitosan microcarrier is uniform in size, the interconnected pores are formed in the surface and the interior, and the highest porosity can reach 95% or higher. With adoption of the porous chitosan microcarrier for culturing cells, the cells can not only be adhered to the surface of the carrier, but also can enter the internal pores to grow, effective cell-cell connection is formed in three-dimensional space, real three-dimensional culture is realized, in vivo environment is effectively simulated, vitality and function of cells are better maintained, and the microcarrier has good biocompatibility.

The invention discloses a method for catalyzing ring-opening polymerization of cyclic ester by a zinc catalyst, and belongs to the technical field of polymer synthetic materials. The problems that anexisting zinc catalyst for catalyzing ring opening of cyclic ester needs the participation of support ligands, and reaction activity and controllability are improved by changing steric hindrance and electrical property of central metal are solved. According to the invention, under the catalysis of a bis(hexaalkyldisilazane)zinc catalyst, lactone is initiated by an alcohol compound to carry out a ring-opening polymerization reaction, and a polyester material with rich structure is obtained. The catalyst adopted by the invention is a green and environment-friendly bis(hexaalkyldisilazane)zinc catalyst which does not need ligand participation, efficient and high-controllable active polymerization of different lactones can be realized under mild conditions, and the monomer universality is verygood, so that various polyester materials with different structures are obtained. The system has good universality for ring-opening polymerization of cyclic ester monomers, catalyzes the synthesis ofvarious degradable polyester materials, and promotes the development of sustainable polymers.

The invention discloses a biomedical degradable magnesium-based bulk amorphous alloy and a preparation method thereof. The chemical composition of the alloy is MgaZnbCacSrd, wherein the atomic percentage of a is 60-73, the atomic percentage of b is 22-35, the atomic percentage of c is 2-6, the atomic percentage of d is 0-2, and a+b+c+d = 100. The Mg-Zn-Ca-Sr bulk amorphous alloy has (1) high amorphous forming ability, (2) good mechanical biocompatibility, high specific strength and low Young's modulus, and (3) suitable corrosion rate and excellent biological compatibility. The amorphous formation critical size is 6mm. The biomedical degradable magnesium-based bulk amorphous alloy has a wide application prospect in the field of biological materials, medical equipment and structural materials.

The invention relates to a method for preparing polyester by catalyzing cyclic anhydride/epoxide ring opening alternate copolymerization with potassium acetate. According to the method, potassium acetate catalyzes an epoxy compound and cyclic anhydride to be subjected to ring-opening alternate copolymerization to synthesize polyester, wherein potassium acetate is used as a catalyst to catalyze thecopolymerization of the cyclic anhydride and the epoxy compound, the reaction formula is defined in the specification, R1 and R2 are anhydride substituents, are the same or different, and simultaneously or independently are a hydrogen atom, a double bond, a benzene ring or a norbornene ring, and R3 and R4 are epoxy compound substituents, and are simultaneously or independently H, methyl, benzenering or cyclohexyl. The preparation method comprises the following steps: carrying out a polymerization reaction on excessive epoxide, cyclic anhydride and potassium acetate by using a normal-pressurebulk polymerization method, precipitating the polymer, filtering, and drying. Compared with the existing metal organic catalyst and non-metal organic catalyst, the potassium acetate provided by the invention has low biotoxicity, low price and simple preparation method, and has great application prospects.

The invention aims at providing an emulsifier for oil-based drilling fluid. The emulsifier is selected from any two or three types of waste oil and grease, a saponified substance of the waste oil and grease and amidate of the waste oil and grease, wherein the saponified substance of the waste oil and grease is obtained through reacting the waste oil and grease with alkali, and the amidate of the waste oil and grease is obtained through reacting the waste oil and grease with organic amine under the catalysis of alkali. The invention further provides a preparation method of the emulsifier and application of the emulsifier in the oil-based drilling fluid. The emulsifier has a good emulsifying effect; the biological toxicity is low, the cost of the emulsifier for the oil-based drilling fluid can be greatly reduced, the waste oil and grease can be changed into the valuable from the waste, and the environment pollution caused by the waste oil and grease is reduced, so that the environmental protection is facilitated.

The invention discloses application of cysteamine in preparing a medicine for treating cancer. Sensitivity on cancer cells by chemotherapeutics is increased by the cysteamine with low dosage, thereby realizing that cancer cells are effectively killed by the chemotherapeutics with low dosage. The invention greatly improves the medicine effect of the chemotherapeutics and lowers the side effects of chemotherapy.

The invention discloses a treatment system for ferment antibiotics wastewater. The treatment system comprises an adjusting tank, a cavitation air floatation machine, a coagulative precipitation tank,a hydrolysis acidification tank, a UASB reactor, a primary sedimentation tank, an A/O system, a secondary sedimentation tank, a Fenton oxidation reaction box, a reaction sedimentation tank and a sludge thickening tank which are successively connected. The invention further discloses a treatment method for the ferment antibiotics wastewater. The treatment method comprises the following steps: pretreatment: the adjusting tank, the cavitation air floatation machine and the coagulative precipitation tank; biochemical treatment: the hydrolysis acidification tank, the UASB reactor, the primary sedimentation tank, the A/O system and the secondary sedimentation tank; and advanced treatment: the Fenton oxidation reaction box and the reaction sedimentation tank. The combined technology is used for treating the ferment antibiotics wastewater, the biotoxicity of the ferment antibiotics wastewater is reduced, and the biodegradability of the wastewater is improved, so that the wastewater is discharged after reaching a standard, the treatment technology is simple, and the operating cost is low.

The invention relates to a preparation method of a fluorescent carbon dot capable of imaging RNA in a living cell for a long time in a targeting manner and a product and application of the preparationmethod, the fluorescent carbon dot is hydro-thermally synthesized from m-phenylenediamine, an amine compound and water as the raw materials; the synthesis method is simple, the conditions are controllable, the prepared carbon dot has the performances of strong green fluorescence emission, ultralow biotoxicity and strong photobleaching resistance, can image the RNA in the cell for a long time, canserve as an RNA probe to distribute and locate the RNA in the conventional cell, or is applied to the dynamic change of the RNA in the cell, and can further be used to indicate the state of the celland screen an anti-cancer drug taking RNA polymerase I as a target spot.

The invention provides a nanometer genetic medicine resistant to tumors and a preparation method and application thereof. The nanometer genetic medicine resistant to tumors comprises amphipathic cationic polymers, acid response functional molecules and small interference RNA, wherein the acid response functional molecules are coupled with the amphipathic cationic polymers, and the small interference RNA adheres to the surfaces of the amphipathic cationic polymers through electrostatic adsorption. The provided nanometer genetic medicine resistant to tumors is great in biocompatibility, low in toxic and side effect and high in bioavailability and has acid pH responsiveness and electric charge overturning performance; the nanometer genetic medicine resistant to tumors has a broad applicationprospect and a high application value in preparation of medicine resistant to tumors or in preparation of medicine for treating vascular normalization.

The invention relates to gadolinium doped zinc oxide nanoparticles and a preparation method thereof. The gadolinium doped zinc oxide nanoparticles are characterized in that the gadolinium doped zinc oxide nanoparticles are evenly dispersed nanoparticles with diameter of 2-6 nm; fluorescence spectrum shows that the nanoparticles have excitation wavelength of 320-360 nm and emission wavelength of 500-560 nm; the excitation wavelength and the emission wavelength show redshift with the increase of the doping amount of gadolinium; the fluorescence intensity first increases then decreases with the increase of the doping amount of gadolinium; and when a molar ratio of zinc to gadolinium is at 1:0.08, the fluorescence enhancement reaches the maximum. The product has application prospect in magnetic resonance and fluorescence double functional imaging. The invention has the advantages that a cheap zinc material with good biocompatibility is employed for direct synthesis of gadolinium doped zinc oxide nanoparticles through a sol-gel method, the method on the one hand reduces the toxicity of the final product, and on the other hand reduces the cost of the product, and the product has application prospect in magnetic resonance and fluorescence double functional imaging.