Polyphenol cross-linking agent and application thereof to preparation of anti-calcification biovalve

A technology of polyphenolic compounds and biological valves, applied in the field of biomedical engineering, to achieve the effects of reducing tissue calcification, low cost, and preventing arteriosclerosis

Active Publication Date: 2018-09-07
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the defects in the prior art, the object of the present invention is to provide a polyphenolic cross-linking agent and its application in the preparation of anti-calcification biological valves, so that the biological heart valve material modified by polyphenolic natural compounds has Better mechanical properties and stability can significantly reduce tissue calcification, inflammation and thrombus, and reduce biological toxicity, thereby greatly extending the service life and overcoming the serious calcification and short service life of biological heart valve materials processed by traditional glutaraldehyde cross-linking methods. defect

Method used

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  • Polyphenol cross-linking agent and application thereof to preparation of anti-calcification biovalve
  • Polyphenol cross-linking agent and application thereof to preparation of anti-calcification biovalve
  • Polyphenol cross-linking agent and application thereof to preparation of anti-calcification biovalve

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preparation example Construction

[0034] Wherein, the preparation method of the completely decellularized bio-based material comprises steps:

[0035] S1: Obtain fresh bio-based materials, and store fresh bio-based materials in a storage transport solution at 2-10°C; the storage transport solution is PBS solution containing penicillin and streptomycin and / or PBS solution containing penicillin and streptomycin D-Hanks solution; in the PBS solution containing penicillin and streptomycin or the D-Hanks solution containing penicillin and streptomycin, the concentration of penicillin is 100U / mL, and the concentration of streptomycin is 0.1mg / mL; The base material includes, but is not limited to, one or more of porcine pericardium, bovine pericardium, small intestine, swim bladder and mesentery;

[0036] Within 4 hours, in a sterile environment, peel off the surface fat of the bio-based material, and then rinse it several times with deionized water, PBS solution and / or D-Hanks solution;

[0037] S2: The bio-based m...

Embodiment 1

[0042] This embodiment provides a polyphenolic crosslinking agent, which is prepared by dissolving curcumin in an organic solvent ethanol solution, and then diluting it with PBS phosphate buffer solution. The specific preparation method includes steps:

[0043] Dissolve 0.8 mg of curcumin in 2 mL of absolute ethanol solution, then dilute with 200 mL of PBS phosphate buffer to prepare a 0.004 mg / mL curcumin cross-linking agent solution, and adjust the pH to 7.4;

[0044] Wherein, the formula composition of PBS phosphate buffer saline (1L) is as follows: Sodium chloride (NaCl) 8g, disodium hydrogen phosphate (NaCl) 2 HPO 4 ) 1.42g, potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, potassium chloride (KCl) 0.2g.

Embodiment 2

[0046] This embodiment provides a polyphenol crosslinking agent, which is prepared by dissolving resveratrol in an organic solvent ethanol solution, and then diluting it with PBS phosphate buffer solution. The specific preparation method includes steps:

[0047] Dissolve 100 mg of resveratrol powder in 4 mL of absolute ethanol solution, then dilute with 6 mL of PBS phosphate buffer to prepare a 10.0 mg / mL resveratrol cross-linking agent solution, and adjust the pH to 7.4.

[0048] Wherein, the formula composition of PBS phosphate buffer saline (1L) is as follows: Sodium chloride (NaCl) 8g, disodium hydrogen phosphate (NaCl) 2 HPO 4 ) 1.42g, potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, potassium chloride (KCl) 0.2g.

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Abstract

The invention relates to a polyphenol cross-linking agent and application thereof to preparation of an anti-calcification biovalve. The polyphenol cross-linking agent is prepared in a way that polyphenol compound is dissolved in organic solvent, and then, buffer solution is adopted for dilution, wherein the polyphenol compound is selected from one or multiple of procyanidine, curcumin, resveratrol, puerarin, aloin and aloe-emodin. The preparation method for the anti-calcification biovalve comprises the following steps: dipping a bio-based material which completely removes cells into the polyphenol cross-linking agent to be subjected to cross-linking, and fully cleaning after ending; dipping in PBS (polybutylene succinate) solution and / or D-Hanks solution to be subjected to post-processingto obtain the anti-calcification biovalve. The anti-calcification biovalve provided by the invention has better mechanical property and stability, tissue calcification, inflammation and thrombus can be obviously reduced, biotoxicity is lowered, service life is greatly prolonged, and the defects of serious calcification, short service life and the like of biological cardiac valve materials processed with a traditional glutaraldehyde crosslinking method are overcome.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a polyphenol cross-linking agent and its application in preparing anti-calcification biological valves. Background technique [0002] In recent years, the incidence of heart valve diseases has continued to increase worldwide. In the United States, 80,000 artificial heart valve replacement operations are performed every year, and about 20,000 people die directly from the disease. Clinically, the conventional treatment plan for valvular heart disease is mainly artificial mechanical valve and artificial biological valve replacement. Patients using artificial mechanical valve need long-term anticoagulant therapy, and the probability of postoperative thrombosis and anticoagulant complications is relatively high. Therefore, In recent years, it has been gradually replaced by artificial biological valves. The artificial biological valve is mainly derived from bovine peric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/00A61L27/36A61L27/54
CPCA61L27/00A61L27/3633A61L27/50A61L27/54A61L2300/42A61L2300/422A61L2430/20A61L2430/40
Inventor 刘婧王志红秦怡博冷希岗孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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