149 results about "Swim bladder" patented technology

The invention provides a method for preparing antioxidant oligopeptide by performing ultrasonic enzymolysis on swim bladders. The method comprises the following steps of: crushing the swim bladders into swim bladder powder; adding a phosphate buffer solution with the pH value of 6.0 and trypsin into the swim bladder powder and stirring uniformly to obtain a swim bladder solution; performing enzymolysis on the swim bladder solution in water bath; performing ultrasonic treatment on the swim bladder solution for enzymolysis; after ultrasonic treatment, continuously performing enzymolysis in constant-temperature water bath; after the enzymolysis is finished, deactivating enzyme, cooling and filtering to obtain supernate; performing ultrafiltration on the supernate by using a 10,000-Da ultrafiltration membrane; and freeze-drying the solution obtained after ultrafiltration to obtain the swim bladder antioxidant oligopeptide. The antioxidant oligopeptide prepared by the method has a good effect of eliminating free radicals of a human body, can prevent ageing, inflammation and heart cerebrovascular diseases caused by arteriosclerosis, and can serve as a nutritional additive to be applied to food.

The invention discloses an intelligent feeding method based on a growth model and sonar feedback. The intelligent feeding method comprises the following steps: S1, establishing a growth model and a feeding prediction model of a fed group; S2, acquiring average specification data of fed groups by the aid of sonar systems before the feed is fed; S3, according to the growth model, the feeding prediction model and the average specification data of the fed group, formulating the feeding amount of the day, and determining the basic feeding amount of each meal in combination with the feeding frequency; s4, feeding the fed group according to the basic feeding amount of each meal, and meanwhile, detecting the number of swim bladder echoes in the feeding process of the fed group by utilizing a sonarsystem; S5, performing analysis processing based on a given algorithm on the echo number of the fish, and grading the number of feeding objects; s6, making a feeding decision according to the aggregation number of the fed groups; and S7, controlling the feeding rate and the feeding amount of the fed group according to the feeding decision.

The invention relates to a method for constructing an air bladder cell system of blotchy rockcod, which comprises: taking an air bladder tissue of the blotchy rockcod as a material to start primary culture, and culturing the air bladder tissue in a DMEM / F12 liquid medium which contains fetal calf serum, carboxymethyl chito-oligosaccharide, basic fibroblast growth factors, I-type insulin-like growth factors, N-acetyl glucose hydrochloride and culture supernatant of heart cells of the blotchy rockcod in the log phase and has a pH value of between 7.0 and 7.4; and adopting a trypsin digestion method for subculturing. The air bladder cell system of the blotchy rockcod constructed by the method is subcultured for 62 generations currently. The technology is scientific and reasonable, is hopeful to be applied to ecotoxicological research, performs pollution monitoring and safety evaluation on various environmental pollutants in the sea, and can also be applied to separation, identification and in vitro breeding of fish viroids, and research in the aspects of interaction of viruses and host cells, and so on.

The invention discloses a process of utilizing a sturgeon raw material to extract collagen. The process includes following steps: 1), selecting sturgeon skin and / or swim bladder which is washed cleanas a raw material, slicing, adding into 5-10wt% sodium hydroxide solution for soaking for 5-10h, filtering, and washing the material clean; 2), adding a material obtained in the step 1) into a defatting mixed solution for defatting for 1-2h, performing ultrasonic treatment during defatting, and washing clean after ultrasonic treatment is completed; 3), adding a material obtained in the step 2) into acid for swelling, homogenizing, using a sodium hydroxide solution to adjust pH to 4.0-5.5, adding protease for enzymolysis, adding yeast powder after enzymolysis is completed, controlling temperature at 30-35 DEG C, stirring for reaction for 2h, and performing enzyme deactivation; 4), discoloring enzymolysis liquid obtained after enzyme deactivation in the step 3), centrifuging, purifying, concentrating, and spray-drying to obtain collagen powder. The process can quickly extract collagen, and the collagen extracted by the process is high in purity, small in molecular weight and easy to absorb.

The invention provides an air supplement device used for a settleable fish-culturing net cage. The air supplement device comprises an air supplement cover located in the interior of a settleable net cage, an air supplement pipe, an air supplement hose, a bait feeding pipe and a bait feeding hose, wherein the air supplement cover is of an umbrella shape or a bell shape; the lower end of the air supplement pipe is positioned in the interior of the air supplement cover; the upper end of the air supplement pipe is connected with the lower end of the air supplement hose; the upper end of the air supplement hose is connected with an inflation pump arranged on a working platform on water surface; air supplied by the inflation pump is filled into the air supplement cover through the air supplementhose and the air supplement pipe; the lower end of the bait feeding pipe is connected with the upper part of the air supplement cover; the upper end of the bait feeding pipe is connected with the bait feeding hose; the upper end of the bait feeding hose is located above the water surface; the lower end of the bait feeding hose is connected with the bait feeding pipe; and sinkable feed particles added from the upper end of the bait feeding hose pass through the bait feeding hose, successively enter the bait feeding pipe and the air supplement cover, and fall into water located below the air supplement cover. The air supplement device provided by the invention can supplement lost air in a swim bladder for certain fish needing direction suction of air during long-term sink-cage culturing; and in order to tame cultured fish to contact air in the air supplement cover, the bait feeding pipe can be used to add the sinkable feed particles into the air supplement cover.