Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease

A neutral protease and fish oil technology, which is applied in the production of fat/fat and fat production, can solve the problems of narrow range of process parameters, high cost, and difficulty in industrial production, and achieve a wide range of process parameters, low cost and difficulty. low effect

Inactive Publication Date: 2014-05-07
JISHOU UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting fish oil from the viscera of almond carp by using neutral protease, which solves the problems that the tec...

Method used

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  • Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease

Examples

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specific Embodiment approach

[0011] The specific embodiment of the present invention comprises the following steps:

[0012] (1) Remove gallbladder, swim bladder and intestinal digesta from the viscera of almond carp, wash, drain and mince into a homogenate;

[0013] (2) Mix the homogenate and water at a weight ratio of 1:1.0~1:3.0, stir evenly, adjust the pH to 6.5~7.0 with hydrochloric acid or sodium hydroxide, and add 2.0%~ 3.0% neutral protease, shake well, raise the temperature to 40-55°C, and carry out the enzymatic hydrolysis reaction for 2.0-3.0 hours.

[0014] (3) Centrifuge the enzymatic solution at 4000-5000r / min for 10-20 minutes, and then divide it into four layers, from top to bottom: fish oil layer, non-enzymatic layer, enzymatic solution layer, and residue Layer, absorb the upper layer to get fish oil.

[0015] Optimally, in step (2), the weight ratio of homogenate to water is 1:1.25, the pH value is 7.0, the amount of neutral protease added is 2.0% of the weight of carp viscera, the enz...

Embodiment 1

[0020] (1) Remove gallbladder, swim bladder and intestinal digesta from the viscera of almond carp, wash, drain and mince into a homogenate;

[0021] (2) Mix the homogenate and water at a weight ratio of 1:1.25, stir evenly, adjust the pH to 7.0 with sodium hydroxide, add 2.0% neutral protease based on the weight of carp viscera, shake well, and heat up to 45 ℃, carry out enzymatic hydrolysis reaction for 2.0h;

[0022] (3) The enzymatic solution was centrifuged at 4500r / min for 15 minutes, and after centrifugation, it was divided into four layers, from top to bottom: fish oil layer, non-enzymatic layer, enzymatic solution layer, and residue layer, Absorb the upper layer to get the fish oil.

[0023] The extraction rate of crude fish oil was 92.13%. The analysis results of its physical and chemical properties are shown in Table 4 Crude Fish Oil Sample 1. It can be seen that the fish oil extracted by this method has reached the secondary standard of crude fish oil.

Embodiment 2

[0025] The operation steps are the same as in Example 1, and the process parameters are: material-to-liquid ratio 1:1.5, pH to 7.0, based on the weight of carp offal, add 2.5% neutral protease, shake well, heat up to 55°C, and carry out enzymatic hydrolysis reaction for 3.0 hours ; The extraction rate was 92.02%. The analysis results of its physical and chemical properties are shown in Table 1, fish oil sample 2. The extracted fish oil has reached the secondary standard of fish oil. The experimental data are shown in Table 1 below:

[0026] Table 1

[0027]

[0028] The grading standard of fish oil in the above table refers to SC / T3502-2000.

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Abstract

The invention discloses a method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease. The method comprises the following steps: 1, removing the gall bladder, swim bladder and digests in the intestinal tract in the internal organs of elopichthys bambusa, cleaning, draining and mincing into the homogenate; 2, mixing the homogenate and water according to a weight ratio of (1:1.0)-(1:3.0), uniformly stirring, adding hydrochloric acid or sodium hydroxide to regulate the pH value to be 6.5-7.0, adding the neutral protease which has 2.0-3.0 percent of the weight of the homogenate based on the weight of the homogenate, uniformly mixing, performing enzymatic hydrolysis in the temperature range of 40-55 DEG C for 2.0-3.0 hours, and forming enzymatic hydrolysate; and 3, filling the enzymatic hydrolysate in a container, and centrifuging under the condition of 4000-5000r/min for 10-20 minutes through a centrifugal machine, wherein the centrifuged enzymatic hydrolysate is divided into four layers which are sequentially a fish oil layer, a non-enzymolysis layer, an enzymatic hydrolysate layer and a residue layer from top to bottom, and the fish oil on the uppermost layer is the final fish oil. The method has the beneficial effects that the technology for extracting the fish oil from the internal organs of elopichthys bambusa is wide in technical parameter range, the difficulty of industrial production is low, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of animal oil extraction, and relates to a method for extracting fish oil from the viscera of almond carp by using neutral protease. Background technique [0002] Fish oil is rich in n-3 series polyunsaturated fatty acids and has high nutritional value. Among them, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are not only one of the important components to maintain cell life activities, but also have the functions of preventing myocardial infarction, arrhythmia, lowering blood lipids, and atherosclerosis. , coronary arteriosclerosis, anti-oxidation scavenging free radicals, anti-aging and other physiological functions. In recent years, with the recognition of the nutritional value of fish oil, the development and utilization of fish oil products has become a research hotspot at home and abroad, and is widely used in food, medicine, feed, chemical and other industries. [0003] The carp belong...

Claims

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Application Information

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IPC IPC(8): C11B1/00
Inventor 罗庆华向建国王海磊王苗苗吴沛霖
Owner JISHOU UNIVERSITY
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