Method for preparing beluga swimming bladder protein peptide
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technique for swimming bladder and squid is applied in the field of preparation of squid bladder protein peptides, and can solve the problems of unsuitability for direct consumption and not being well utilized, etc.
Inactive Publication Date: 2015-01-28
HARBIN PATERNA BIOTECH DEV
View PDF3 Cites 8 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The swim bladder of the sturgeon fish is relatively large and has two vertical bands, accounting for about 5% of the weight of the sturgeon fish. It is rich in collagen, but its texture is tough an...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0008] Take fresh beluga swim bladder, cut it open, remove the blood vessels and mucous membrane, flatten it, rinse it with clean water, drain it, and chop it; weigh 1 kg of the chopped fish bladder, add 5 L of 0.5% sodium carbonate aqueous solution, and soak for 3 hours , centrifuge at 3500rpm for 10min, wash the precipitate repeatedly with water until the pH is neutral; add 5L of 0.2% hydrochloric acid aqueous solution, soak for 2h, centrifuge at 3500rpm for 10min, wash the precipitate repeatedly with water until the pH is neutral; add 10L of pure water , heated to 90°C, extracted for 6 hours under constant temperature stirring, and filtered; cooled the filtrate to 50°C, adjusted the pH to 9, added 50g of alkaline protease, hydrolyzed at 50°C for 4h, inactivated the enzyme at 95°C for 5min, and cooled to Adjust the pH to 2.5 at 45°C, add 30g of acidic protease, hydrolyze at 45°C for 5 hours, inactivate the enzyme at 95°C for 5 minutes, adjust the pH to neutral, filter, desalt...
Embodiment 2
[0010] Take a fresh beluga swim bladder, cut it open, remove blood vessels and mucous membranes, flatten it, rinse it with clean water, drain it, and chop it up; weigh 500g of the chopped swim bladder, add 2500mL of 0.5% sodium carbonate aqueous solution, soak for 3 hours, Centrifuge at 3500rpm for 10min, wash the precipitate repeatedly with water until the pH is neutral; add 2500mL of 0.2% hydrochloric acid aqueous solution, soak for 2h, centrifuge at 3500rpm for 10min, wash the precipitate repeatedly with water until the pH is neutral; add 5000mL of pure water, Heat to 90°C, under constant temperature stirring, extract for 6h, filter; cool the filtrate to 50°C, adjust the pH to 9, add 25g of alkaline protease, enzymatically hydrolyze at 50°C for 4h, inactivate the enzyme at 95°C for 5min, cool to 45°C ℃, adjust the pH to 2.5, add 15g of acidic protease, hydrolyze at 45℃ for 5h, inactivate the enzyme at 95℃ for 5min, adjust the pH to neutral, filter, desalt the filtrate, conce...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention provides a method for preparing a beluga swimming bladder protein peptide. The method specifically comprises the following steps: treating and shredding fresh beluga swimming bladders, adding 5-fold dilute alkali liquor, soaking for 3 hours, performing centrifugal separation, washing the precipitate until the pH value is neutral; adding a 5-fold dilute acid solution, soaking for 2 hours, performing centrifugal separation, and washing the precipitate until the pH value is neutral; adding 10-fold water, extracting at the temperature of 90 DEG C for 6 hours, and filtering; cooling to 50 DEG C, regulating the pH value to 9, adding 0.5 percent of alkaline protease, performing enzymolysis at the temperature of 50 DEG C for 4 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; cooling to 45 DEG C, regulating the pH value to be 2.5, adding 0.3 percent of acid proteinase, performing enzymolysis at the temperature of 45 DEG C for 5 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; and regulating the pH value to be neutral, filtering, desalting, concentrating, and drying, thereby obtaining the beluga swimming bladder protein peptide.
Description
technical field [0001] The invention relates to a protein processing method, in particular to a preparation method of beluga fish swim bladder protein peptide. Background technique [0002] The sturgeon is a large carnivorous fish that only exists in Heilongjiang, the border river between China and Russia. The beluga has a huge body and is a large fish, known as the "king of freshwater fish". The whole body of the sturgeon fish is full of treasures. Its meat is thick and thornless, and its taste is delicious and fresh. The swim bladder of the sturgeon fish is relatively large and has two vertical bands, accounting for about 5% of the weight of the sturgeon fish. It is rich in collagen, but its texture is tough and not easy to tear, so it is not suitable for direct consumption. Therefore, it has not been well received. Utilization requires processing to be better digested and absorbed by the human body. Contents of the invention [0003] The invention provides a prepara...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more