Method for preparing beluga swimming bladder protein peptide

Abstract

The invention provides a method for preparing a beluga swimming bladder protein peptide. The method specifically comprises the following steps: treating and shredding fresh beluga swimming bladders, adding 5-fold dilute alkali liquor, soaking for 3 hours, performing centrifugal separation, washing the precipitate until the pH value is neutral; adding a 5-fold dilute acid solution, soaking for 2 hours, performing centrifugal separation, and washing the precipitate until the pH value is neutral; adding 10-fold water, extracting at the temperature of 90 DEG C for 6 hours, and filtering; cooling to 50 DEG C, regulating the pH value to 9, adding 0.5 percent of alkaline protease, performing enzymolysis at the temperature of 50 DEG C for 4 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; cooling to 45 DEG C, regulating the pH value to be 2.5, adding 0.3 percent of acid proteinase, performing enzymolysis at the temperature of 45 DEG C for 5 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; and regulating the pH value to be neutral, filtering, desalting, concentrating, and drying, thereby obtaining the beluga swimming bladder protein peptide.

Description

technical field [0001] The invention relates to a protein processing method, in particular to a preparation method of beluga fish swim bladder protein peptide. Background technique [0002] The sturgeon is a large carnivorous fish that only exists in Heilongjiang, the border river between China and Russia. The beluga has a huge body and is a large fish, known as the "king of freshwater fish". The whole body of the sturgeon fish is full of treasures. Its meat is thick and thornless, and its taste is delicious and fresh. The swim bladder of the sturgeon fish is relatively large and has two vertical bands, accounting for about 5% of the weight of the sturgeon fish. It is rich in collagen, but its texture is tough and not easy to tear, so it is not suitable for direct consumption. Therefore, it has not been well received. Utilization requires processing to be better digested and absorbed by the human body. Contents of the invention [0003] The invention provides a prepara...

AI Technical Summary

Problems solved by technology

The swim bladder of the sturgeon fish is relatively large and has two vertical bands, accounting for about 5% of the weight of the sturgeon fish. It is rich in collagen, but its texture is tough and not easy to tear, so it is not suitable for direct consumption. Therefore, it has not been well received. Utilization, need to be processed in order to be better digested and absorbed by the human body