12 results about "Acid protein" patented technology

The application discloses a vaccine and a preparation method thereof, and the preparation method comprises the following steps: mixing an acidic protein antigen solution containing a phosphate buffer solution with an aluminum adjuvant to make the molar ratio of phosphorus and aluminum below 0.9, so as to obtain an acidic protein vaccine; or mixing the aluminum adjuvant with a solution containing phosphate ions to make the molar ratio of phosphorus and aluminum above 0.2, and then mixing the aluminum adjuvant with an alkaline protein antigen solution containing a phosphate buffer solution, so as to obtain an alkaline protein vaccine; wherein the acidic protein antigen is any kind of protein antigen with a net negative charge; and the alkaline protein antigen is any kind of protein antigen with a net positive charge. According to the preparation method, the molar ratio of phosphorus and aluminum is adjusted to a specific range according to the net charge of the protein antigen, so that the isoelectric point of the aluminum adjuvant can be effectively changed, the charge carried by the aluminum adjuvant is opposite to the charge carried by the protein antigen, and then the aluminum adjuvant and the protein antigen are attracted to each other through electrostatic interaction. The vaccine antigen prepared by the method has high antigen adsorption rate and good stability, the preparation method is simple, and the method is suitable for industrial application.

The present application relates to the field of biological detection, in particular to an electrochemiluminescence sensor based on antibody modified nanochannel and a construction method and use method thereof. The electrochemiluminescence sensor based on antibody modified nanochannel comprises an anodic aluminum oxide film with a nanochannel penetrating through the surface, and a hydrogel is attached to one side surface of the anodic aluminum oxide film, which can reduce the problem of easy fragmentation of the anodic aluminum oxide film caused by direct contact operation, and the modifier is completely modified on the hydrogel layer, so that the modifier is separated from the nanochannel, and the problem of easy blockage of the anodic aluminum oxide film nanochannel modified by the antibody is solved. In addition, all the modifiers can be removed by boiling, and the non-directional utilization rate of the anodic aluminum oxide film is improved. The construction method of the electrochemiluminescence sensor based on antibody modified nanochannel can achieve good modification effect in a short time and improve the efficiency. When used in collagen fiber acid protein analysis, the clinical operation efficiency is improved, and rapid, high sensitivity, high accuracy and complete quantitative detection of collagen fiber acid protein are realized.

The invention discloses a drug delivery carrier material, a drug delivery coating and a preparation method thereof, and belongs to the technical field of biological materials. The preparation method of the drug delivery coating comprises the following steps: reacting polypeptide containing hydrophobic amino acid with acidic protein in a solution system in which the pH value is 3-7, the temperature is 20-50 DEG C and an oxidizing agent exists, and mixing the prepared coating with a drug solution to realize drug filling, and then mixing with a phosphate solution to induce the micro-nano pore structure in the coating to be closed. The isoelectric points of the polypeptide and the acidic protein are both less than 7, and the difference value of the isoelectric points is less than or equal to 2.5; the acidic protein is negatively charged under the condition that the pH is greater than or equal to 7. According to the method, the contradiction that high loading and low burst release of an existing carrier are difficult to achieve at the same time is effectively solved, the coating is mild in drug loading condition, effective loading and activity retention of various active biomolecules can be achieved, the coating can also adapt to various drug types through modular design, and a brand new mode is provided for constructing a next-generation intelligent drug delivery system.

The invention discloses electron accelerator equipment for feather meal irradiation, which is characterized in that a feather meal track is provided with an irradiation hall in a matching manner, the irradiation hall is provided with an accelerator, and powder directly falls into a stock bin after being irradiated by the irradiation hall. According to the technical scheme adopted by the invention, the accelerator production line is put, and the traditionally modified feather meal is irradiated, so that the amount of acidic protein is increased.

The invention discloses a Chlamydomonas reinhardtii sourced umami peptide and a preparation, screening and evaluation method thereof. Chlamydomonas reinhardtii powder is used as a raw material to extract total protein, salting-out crude protein is obtained through salting-out, and high-purity acidic protein is obtained through purification; the method comprises the following steps: by taking salting-out crude protein as a substrate, carrying out step-by-step enzymolysis (40DEG C, pH 7.5 and 1h respectively) on flavourzyme (3000U / g) and trypsin (1000U / g), carrying out 10kDa and 3kDa ultrafiltration grading on enzymatic hydrolysate, and determining that a component with acidic protein less than 3kDa is an optimal component (Ka = 38.8067 + / -1.2, and a threshold value is 0.176 mg / mL) by combining an electronic tongue, sensory evaluation and a delicate flavor threshold value. Peptide fragments are identified through LC-MS / MS, 30 non-toxic and non-sensitization potential umami peptides are obtained through virtual screening, and molecular docking verifies that the umami peptides are stably combined with a T1R1-VFT receptor. The process has synergistic interaction, the product is natural and safe, and high-quality raw materials and technical schemes are provided for development of natural seasonings. The abstract drawing is figure 3.

The application relates to the field of antibody detection, in particular to an antibody based on anti-glial fibrillary acidic protein and application thereof. The application provides a nanobody Nb-14 against human glial fibrillary acidic protein, the amino acid sequence of which is shown as SEQ ID NO: 10, the EC 50 = 2.45 x 10 ‑ 6 mol / L, and the endocytosis efficiency in U87MG cells is greater than 90%; qualitative and quantitative detection of glial fibrillary acidic protein can be realized, and the antibody has high specificity and detection sensitivity.

Compositions of aCap virus-ribonucleic acid protein complex conjugates (VRC), wherein a viral capsid protein is covalently linked to a ribonucleic acid protein complex, i.e., a ribonucleoprotein (RNP), for selective delivery of the RNP into a targeted cell nucleus, and their preparation and uses as therapeutics.

The invention discloses a method for promoting fibrosis of plant proteins by a subcritical water technology, which comprises the following steps: (1) dispersing plant proteins in water to obtain a uniform protein dispersion liquid; (2) adjusting the pH value of the protein dispersion liquid to 2.0-2.5 to obtain an acidic protein dispersion liquid; and (3) treating the acidic protein dispersion liquid in a subcritical water environment at 105-120 DEG C for 20-60 minutes to obtain the vegetable protein nanofiber. According to the method, protein is placed under an acidic condition, and a protein space structure is rearranged in a subcritical water environment. In a subcritical water environment, a tertiary structure of the protein is partially or completely expanded, so that a hydrophobic group is exposed. The process promotes the conversion of the protein from a spherical structure to a fibrous structure.

The application belongs to the field of biological medicine, and relates to application of chondroitin acid protein 1 in preparation of a reagent for diagnosing lung adenocarcinoma or evaluating lung adenocarcinoma prognosis. The application screens CRTAC1 from a GEO database through bioinformatics analysis and a machine learning algorithm, and the CRTAC1 is significantly lowly expressed in lung adenocarcinoma tissues, and an AUC reaches 0.972. Verification of a GEPIA database shows that low expression of the CRTAC1 is significantly related to poor prognosis such as advanced pathological stages, and a patient with high expression has better prognosis. The CRTAC1 blocks epithelial-mesenchymal transition through inhibition of an EGF / ErbB signal path, and remodels a tumor immune microenvironment through inhibition of M2 type macrophage polarization. A mouse lung metastasis model proves that overexpression of the CRTAC1 significantly reduces lung metastasis nodules. The application provides a new molecular marker and a drug target for early diagnosis, prognosis evaluation and precise treatment of lung adenocarcinoma.

The application belongs to the technical field of biotechnology, and particularly relates to a preparation method of a multifunctional nanofiber composite carrier with precise intestinal delivery capacity and application thereof, and comprises the following steps: S1, mixing and treating protein raw materials, a pH regulator and potassium ferrocyanide to obtain an acidic protein solution; S2, emulsifying the acidic protein solution with vegetable oil and an emulsifier to obtain an emulsion; and S3, heating the emulsion to obtain the carrier. The preparation method is characterized by simple preparation method, convenient operation, wide application range, easy scale production and the like. The carrier prepared by the preparation method has good stability, good slow-release effect, release responsiveness in different regions of the small intestine and the colon, improvement of constipation and the like, and has SOD-like enzyme activity, is suitable for embedding different types of active substances, is applied to nutrition and health functional food and medicines, and has a wide prospect.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.