64 results about "Acid protein" patented technology

The invention relates to bone health compositions comprising an acidic protein fraction of milk, to a method of producing said bone health composition, to methods of treatment comprising said bone health compositions and to medicinal uses of said bone health compositions. One broad aspect of the invention provides a bone health composition comprising an acidic protein fraction derived from milk, from a component of milk, from whey, from hydrolysates thereof, or from a combination thereof, or from a combination thereof wherein the composition does not comprise caseinoglycomacropeptide (CGMP). Another broad aspect provides a method of manufacturing the composition of the invention using anion exchange chromatography.

The invention discloses a preparation method of an injectable gel material of sodium alga acid-protein adhesive used for treating myocardial infarction, relating to injectable hydrogelin and a preparation method of the hydrogelin. The material and the method solve the technical problems of the existing injectable hydrogelin using calcium ion for crosslinking such as poor compatibility with organisms and undesirable mechanical property. The injectable gel material of sodium alga acid-protein adhesive used for treating myocardial infarction is made from the component sodium alga acid and the component protein adhesive that are mixed. The preparation method includes: adding sodium alga acid in water to oscillate with light avoidance on a table concentrator, thus generating partially oxidized sodium alga acid solution; adding glycol to the solution and placed on the table concentrator to oscillate, then adding sodium chloride to oscillate, dissolve and precipitate, dissolving the precipitate in water to attain collosol, and dialyzing and freeze-drying to obtain partially oxidized sodium alga acid; formulating the sodium alga acid solution to obtain sodium alga acid component; formulating gelatin or collagen to obtain protein adhesive component; and mixing the sodium alga acid component with the protein adhesive component for use. The injectable gel material can be used for treating myocardial infarction.

The invention provides a method for separating protein from yellow slurry of bean curd by using a flocculating agent, comprising the following steps: chitosan and one or more than two of the following mixtures (is) are added into the yellow slurry of bean curd as compound flocculating agent: polymerized aluminium trichloride, polymerized iron sulfate and sodium alginate; the total volume of the flocculating agent is 0.1-3mg/mL; the flocculating agent is first stirred and dispersed, then stirred and mixed mechanically for 5-10 min at 20 DEG C, placed still for 10-24h at the temperature between 0-4 DEG C, centrifugated and precipitated, finally, the protein is gained. In the invention, the combination of the chitosan and other flocculating agents is used as the compound flocculating agent, especially the natural giant molecule amphiprotic flocculating agent-chitosan composition is used, and compared with the traditional synthesized high molecule flocculating agent, the chitosan composition is more environmental friendly, the protein recycling rate is high, bigger molecule copolymer can be formed, the flocculating effect is good, the using amount is small, the cost is low, and the recycled protein product has good quality; in addition, the flocculating agent can be used in acid protein drinks or used as protein extender in the industries of baking, meet product, etc.

The present invention provides a rapid and relatively simple process for purification of acidic proteins expressed in tobacco cells. The process comprises three main purification steps: precipitation with polyethyleneimine, column chromatography with a hydrophobic interaction resin, and column chromatography with hydroxyapatite. The process provides pure or essentially pure protein at a very high yield.

The present invention relates to method of extracting polysaccharide from Hojothuria leucosppilota. The method includes the steps of: alkaline hydrolysis of Hojothuria leucosppilota, enzymolysis, eliminating acid protein, alcohol precipitation, etc. The obtained Hojothuria leucosppilota polysaccharide product contains Hojothuria leucosppilota polysaccharide over 55 wt%. The Hojothuria leucosppilota polysaccharide has the functions of reducing cerebral ischemia, improving cerebral pia mater microcirculation, preventing platelet aggregation and thrombosis, and resisting coagulation, so that the Hojothuria leucosppilota polysaccharide may be used in preparing medicine for treating ischemic cerebral apoplexy and thrombotic diseases.

A cosmetic agent for treating keratin fibers is provided herein. The cosmetic agent includes, in a cosmetically acceptable carrier, in relation to the total weight of the cosmetic agent, from about 0.001 to about 10 wt. % of at least one protein which has a content of glutamic acid and/or aspartic acid of from about 10 to about 60%, in relation to the total content of all amino acids of the protein. The cosmetic agent further includes, in a cosmetically acceptable carrier, in relation to the total weight of the cosmetic agent, from about 0.01 to about 10 wt. % of at least one salt which includes at least one divalent cation, and/or of at least one salt which includes at least one trivalent cation.

The invention provides a method for preparing a beluga swimming bladder protein peptide. The method specifically comprises the following steps: treating and shredding fresh beluga swimming bladders, adding 5-fold dilute alkali liquor, soaking for 3 hours, performing centrifugal separation, washing the precipitate until the pH value is neutral; adding a 5-fold dilute acid solution, soaking for 2 hours, performing centrifugal separation, and washing the precipitate until the pH value is neutral; adding 10-fold water, extracting at the temperature of 90 DEG C for 6 hours, and filtering; cooling to 50 DEG C, regulating the pH value to 9, adding 0.5 percent of alkaline protease, performing enzymolysis at the temperature of 50 DEG C for 4 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; cooling to 45 DEG C, regulating the pH value to be 2.5, adding 0.3 percent of acid proteinase, performing enzymolysis at the temperature of 45 DEG C for 5 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; and regulating the pH value to be neutral, filtering, desalting, concentrating, and drying, thereby obtaining the beluga swimming bladder protein peptide.

There is provided herein a system and method for de-toxifying and de-scaling source water. In some embodiments, source water will be mixed with either an aluminum source or an iron source to separate endotoxins from acidic proteins and convert the naturally present bicarbonate in source water to carbon dioxide. Endotoxins and carbon dioxide will then be removed from source water by a stage of hydrophobic membranes to produce de-toxified and de-carbonated source water. Calcium hydroxide will be mixed with the de-toxified and de-carbonated source water to form precipitates comprising foulants and sulfate. A recoverable and reusable amine solvent will also be used to induce efficient precipitation. Possible reuse applications for the treated source water by the inventive methods that minimize excessive uses of potable water may include hydro-fracturing of shale and sand formations and heavy oil recovery by steam injection.

The invention relates to the field of genetic engineering. In particular, the invention relates to a fungus-derived acid protease g412 as well as a gene and application thereof, and the amino acid sequence of the fungus-derived acid protease g412 is shown as SEQ ID NO.1 or SEQ ID NO.2. The acid protease disclosed by the invention has good properties, and can be applied to industries such as food, feed and pharmacy. According to the technical solution of the invention, a genetic engineering means can be utilized to produce the protease with excellent properties which is suitable for industrial application.

The invention relates to the field of medicine, in particular to a preparation process of human immunoglobulin for intravenous injection. Octanoic acid is used for replacing ethanol as a precipitatingagent, so that acidic protein in blood plasma generates precipitates; IgG molecules with high isoelectric points are reserved on supernate; only through one-step precipitation reaction, the materialscan be transferred to a chromatography working procedure; caprylate is used; lipid envelope viruses are inactivated under the mild condition in short time; even partial non-lipid envelope viruses canbe inactivated; the action time is short; the effect is good; the bioactivity of the immunoglobulin cannot be easily influenced; the product safety is improved; the quality and the yield of the product are ensured; a two-step chromatography working procedure is used; most partial polymers and other impure protein are removed; the pyrogen is reduced; chromoprotein such as ceruloplasmin can be adsorbed; the product appearance is effectively improved; one-step precipitation reaction is used; the loss is reduced; the product yield is improved; the preparation process of human immunoglobulin for intravenous injection has the advantages of high product yield, high purity and high safety.

There is provided herein a system and method for de-toxifing and de-scaling source water. In some embodiments, source water will be mixed with either an aluminum source or an iron source to separate endotoxins from acidic proteins and convert the naturally present bicarbonate in source water to carbon dioxide. Endotoxins and carbon dioxide will then be removed from source water by a stage of hydrophobic membranes to produce de-toxified and de-carbonated source water. Magnesium hydroxide will be mixed with the de-toxified and de-carbonated source water to form precipitates comprising foulants and sulfate. A recoverable and reusable amine solvent will also be used to induce efficient precipitation. Possible reuse applications for the treated source water by the inventive methods that minimize excessive uses of potable water may include hydro-fracturing of shale and sand formations and heavy oil recovery by steam injection.

The present invention discloses separation and purification process of neurotoxin and cytotoxin of cobratoxin, and the process can separate neurotoxin and cytotoxin of cobratoxin simultaneously and raise the comprehensive utilization efficiency of cobratoxin. The process includes first treating cobratoxin liquid with acetone to eliminate liposoluble components from cobratoxin liquid, regulating the pH value of the water solution of cobratoxin and acetone powder with acid, centrifuging to eliminate the acid protein and polypeptide, specifically PAA adsorbing and precipitating the alkali protein and alkali polypeptide in cobratoxin liquid, centrifuging to separate PAA-alkali protein/polypeptide composition and eliminate water soluble components; desorbing PAA-alkali protein/polypeptide with pH variation, and eliminating PAA with calcium chloride; and separating and purifying neurotoxin and cytotoxin of cobratoxin with Sephadex G-50. The product has low salt content and may be frozen directly.

The invention belongs to the technical field of agro-biology and particularly relates to fungus-originated acid protease Bs2688 and gene and application thereof. An amino acid sequence of the acid protease is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The invention provides a new protease gene; proteinases with good character are produced by means of genetic engineering; the new protease gene is applicable to the industries, such as feed, food and medicine.

Hair treatment agents include selected protein hydrolysates and selected complexes of acidic protein hydrolysates and basic fatty acid amidoamines.

A meat or fish composition which retains moisture during cooking is provided. A dry protein mixture or an aqueous acidic protein solution derived from animal muscle tissue is added to the meat or fish prior to cooking. The dry protein mixture and aqueous acidic protein solution comprise myofibrillar proteins and sarcoplasmic proteins substantially free of myofibrils and sarcomeres.

The present invention relates to a compound enzyme preparation for pig feeds. The compound enzyme preparation is characterized by comprising the following raw materials in parts by weight: 22-27 partsof acid cellulase, 8-12 parts of glucanase, 23-27 parts of medium-temperature alpha-amylase, 23-27 parts of acid protease, 8-12 parts of saccharifying enzyme and 3-6 parts of lipase. The compound enzyme preparation for the pig feeds improves the digestion utilization rate of plant fibers and proteins, improves the utilization rate of the feeds, can effectively increase the immunity and digestionand absorption of pig bodies, reduces morbidity and improves ecological environment.

The invention belongs to the technical field of agrobiology, and particularly relates to a fungus-derived acid protease Bs2688 mutant and a gene and application thereof. The amino acid sequence of theprotease provided by the invention is shown in SEQ ID NO. 3. The invention provides a novel protease gene, and the protease with excellent properties is produced by using a genetic engineering meansand is applied to a feed, food, medicine and other industries.

The invention discloses a method for efficiently expressing acid protease and application thereof, and belongs to the field of gene engineering. The expression enzyme activity of the recombinant pichia pastoris P.pastoris GS115/pPIC9K-en-alpha SP-acidprotea provided by the invention reaches 5395U/mL and is improved by 109% compared with the enzyme activity of a control strain containing original alpha-signal peptide; the acidic protein disclosed by the invention is in a pH range of 1-5, the enzyme can maintain the enzyme activity of 60% or more, the residual enzyme activity is 80% or more after the enzyme is treated at 20-40 DEG C for 30 minutes, and the acid protease can be suitable for industries such as food, feed or brewing and the like. According to the technical scheme provided by the invention, the acid protease suitable for industrial application can be produced by utilizing a genetic engineering means.

The present invention relates to a nano compound condom, Every condom contains a solid metal cation carrier material which is formed from (NaAg)2ZrPO4 or (NaNn)2ZrPO4 or (NaCu)2ZrPO4 or their mutually-combined product and whose microparticle size is below 0.1 micrometer and whose weight is 0.01-0.8 of that of emulsion. Its released metal cation can change the chemical composition of bacterial mechanism, and can break the acid protein on which the bacteria depend for existance, can kill AIDS vines and various VD pathogens, and can prevent cross-infection.

The invention provides a human disease associated acidic protein and polynucleotides which identify and encode DAAP. The invention also provides expression vectors, host cells. agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of DAAP.

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of a compound bone peptide injection. The preparation method provided by the invention comprises the following steps: preparing a bone peptide extracting solution and a scorpion extracting solution, mixing the bone peptide extracting solution with the scorpion extracting solution, performing acid treatment, alkali treatment and activated carbon treatment on the mixed solution, performing filtering, adding an additive, and further performing treating. According to the invention, a production process is innovated, and acid protein and basic protein are treated in the step of preparing the bone peptide extracting solution. Acid treatment and neutral filtration are performed in the step of preparing the scorpion extracting solution, and finally, the additive is added, so that the prepared bone peptide extracting solution is high in medicine stability, the problem that the clarity is reduced after long-term storage is solved, polypeptide substances are larger than or equal to 30mg/ml, and total nitrogen is larger than or equal to 10mg/ml; and according to the scorpion extracting solution, polypeptide substances are larger than or equal to 0.5mg/ml, total nitrogen is larger than or equal to 0.25mg/ml, and the scorpion extracting solution is far superior to similar products.

The invention relates to a preparation method of high-density yeast fermentation liquor. The method comprises the following steps: performing size mixing on corn, soybean meal and water, respectivelyadding high-temperature amylase, glucoamylase and acidic protein for thorough enzymolysis, and carrying out solid-liquid separation to obtain solid wet residues and a diluted composite nutrient solution; diluting a part of the diluted composite nutrient solution, inoculating the diluted solution with a yeast seed solution for culturing, and carrying out reduced-pressure evaporation on the residualdiluted composite nutrient solution to obtain a concentrated solution; and in the culture process, controlling sufficient dissolved oxygen in a fed-batch manner of the concentrated composite nutrientsolution and nutritive salt, stopping feeding when the yeast density is maximum, acquiring yeast fermentation liquor when the fermentable sugar is used up, and performing standing to obtain high-density yeast liquid. According to the invention, different enzyme preparations are used for enzymolysis to obtain sugar and amino acid required by yeast growth, and the high-density yeast liquid is obtained in a continuous fed-batch fermentation and batch discharge manner, so that the yeast content is increased, the fermentation and sterilization steam consumption is small, the production water consumption is low, the discharge amount of tank washing wastewater for a fermentation tank is reduced, and the effects of energy conservation, consumption reduction and clean production are achieved.

The invention belongs to the technical field of formaldehyde scavengers and in particular relates to a compound protein formaldehyde scavenger as well as a preparation method and application of the compound protein formaldehyde scavenger. The compound protein formaldehyde scavenger comprises the following raw materials: 10-20% of plant proteins, 0.2-1.0% of acidic proteinase, 10-20% of animal proteins, 0.2-1.0% of alkaline protease, 0.2-1.0% of oxidant, 0.2-1.0% of decolorizing agent, 0.2-2.0% of penetrating agent, 5-15% of humectant and 40-70% of water. The compound protein formaldehyde scavenger contains a great amount of amino acid derivatives, biological enzymes and the like, the amino acid derivatives react with formaldehyde, the compound protein formaldehyde scavenger is good in chemical stability, nontoxic, tasteless and nonvolatile and incapable of generating secondary pollution due to certain antibacterial property, and the biological enzymes and the like adsorb formaldehyde to play a clearing role; and the compound protein formaldehyde scavenger is relatively low in raw material cost, simple and convenient in preparation method, wide in application range, green, environment-friendly and higher in formaldehyde removal efficiency due to the combination of the plant proteins and the animal proteins.