69 results about "Acid protein" patented technology

The invention relates to bone health compositions comprising an acidic protein fraction of milk, to a method of producing said bone health composition, to methods of treatment comprising said bone health compositions and to medicinal uses of said bone health compositions. One broad aspect of the invention provides a bone health composition comprising an acidic protein fraction derived from milk, from a component of milk, from whey, from hydrolysates thereof, or from a combination thereof, or from a combination thereof wherein the composition does not comprise caseinoglycomacropeptide (CGMP). Another broad aspect provides a method of manufacturing the composition of the invention using anion exchange chromatography.

The present invention provides a method of removing product-related inactive or partially active species, high molecular weight aggregates, as well as other process-related impurities from preparations of acidic proteins by using ceramic hydroxyapatite chromatography.

A peptide composition derived from a protein composition when added to uncooked food effects moisture retention during cooking of the food. The peptide composition is derived from a dry protein mixture or an aqueous acidic protein solution which, in turn, is derived from animal muscle tissue. The peptide composition is added to uncooked poultry, meat or fish prior to cooking. The dry protein mixture and aqueous acidic protein solution comprise myofibrillar proteins and sarcoplasmic proteins substantially free of myofibrils and sarcomeres.

The invention discloses a preparation method of an injectable gel material of sodium alga acid-protein adhesive used for treating myocardial infarction, relating to injectable hydrogelin and a preparation method of the hydrogelin. The material and the method solve the technical problems of the existing injectable hydrogelin using calcium ion for crosslinking such as poor compatibility with organisms and undesirable mechanical property. The injectable gel material of sodium alga acid-protein adhesive used for treating myocardial infarction is made from the component sodium alga acid and the component protein adhesive that are mixed. The preparation method includes: adding sodium alga acid in water to oscillate with light avoidance on a table concentrator, thus generating partially oxidized sodium alga acid solution; adding glycol to the solution and placed on the table concentrator to oscillate, then adding sodium chloride to oscillate, dissolve and precipitate, dissolving the precipitate in water to attain collosol, and dialyzing and freeze-drying to obtain partially oxidized sodium alga acid; formulating the sodium alga acid solution to obtain sodium alga acid component; formulating gelatin or collagen to obtain protein adhesive component; and mixing the sodium alga acid component with the protein adhesive component for use. The injectable gel material can be used for treating myocardial infarction.

The invention provides a method for separating protein from yellow slurry of bean curd by using a flocculating agent, comprising the following steps: chitosan and one or more than two of the following mixtures (is) are added into the yellow slurry of bean curd as compound flocculating agent: polymerized aluminium trichloride, polymerized iron sulfate and sodium alginate; the total volume of the flocculating agent is 0.1-3mg / mL; the flocculating agent is first stirred and dispersed, then stirred and mixed mechanically for 5-10 min at 20 DEG C, placed still for 10-24h at the temperature between 0-4 DEG C, centrifugated and precipitated, finally, the protein is gained. In the invention, the combination of the chitosan and other flocculating agents is used as the compound flocculating agent, especially the natural giant molecule amphiprotic flocculating agent-chitosan composition is used, and compared with the traditional synthesized high molecule flocculating agent, the chitosan composition is more environmental friendly, the protein recycling rate is high, bigger molecule copolymer can be formed, the flocculating effect is good, the using amount is small, the cost is low, and the recycled protein product has good quality; in addition, the flocculating agent can be used in acid protein drinks or used as protein extender in the industries of baking, meet product, etc.

The invention discloses a method for preparing bone peptide extract. The method comprises the following steps: (1) pig limb bones are smashed and added to water for hot-pressing extraction, and extract solution is concentrated so as to obtain concentrated solution on standby; (2) acid precipitation is carried out, which is to add dilute hydrochloric acid to the concentrated solution for acid precipitation; (3) alkali precipitation is carried out, which is to filter acid precipitation liquid and add lye to filtrate for alkali precipitation; (4) alcohol precipitation is carried out, which is to filter alkali precipitation liquid, add the dilute hydrochloric acid to the filtrate, reduce pressure, concentrate, cool, add ethanol in, keep stand for more than 12 hours at a temperature between 0 and 5 DEG C and obtain liquid medicine on standby; and (5) ultrafiltration is carried out, which is to ultrafilter the liquid medicine obtained in a step (4), collect ultrafiltrate and well mix the ultrafiltrate. The method has the advantages that the method extracts the pig limb bones and then carries out concentration, thereby providing convenience for production and reducing cost; the method respectively adopts acid precipitation, alkali precipitation and alcohol precipitation to remove acidic protein, basic protein and neutral protein, thereby speeding up ultrafiltration; the method adopts ultrafiltration to guarantee the thorough removal of impure protein and effectively control high molecular weight matter; and the method guarantees clinical safety.

The present invention provides a rapid and relatively simple process for purification of acidic proteins expressed in tobacco cells. The process comprises three main purification steps: precipitation with polyethyleneimine, column chromatography with a hydrophobic interaction resin, and column chromatography with hydroxyapatite. The process provides pure or essentially pure protein at a very high yield.

The present invention relates to method of extracting polysaccharide from Hojothuria leucosppilota. The method includes the steps of: alkaline hydrolysis of Hojothuria leucosppilota, enzymolysis, eliminating acid protein, alcohol precipitation, etc. The obtained Hojothuria leucosppilota polysaccharide product contains Hojothuria leucosppilota polysaccharide over 55 wt%. The Hojothuria leucosppilota polysaccharide has the functions of reducing cerebral ischemia, improving cerebral pia mater microcirculation, preventing platelet aggregation and thrombosis, and resisting coagulation, so that the Hojothuria leucosppilota polysaccharide may be used in preparing medicine for treating ischemic cerebral apoplexy and thrombotic diseases.

A cosmetic agent for treating keratin fibers is provided herein. The cosmetic agent includes, in a cosmetically acceptable carrier, in relation to the total weight of the cosmetic agent, from about 0.001 to about 10 wt. % of at least one protein which has a content of glutamic acid and / or aspartic acid of from about 10 to about 60%, in relation to the total content of all amino acids of the protein. The cosmetic agent further includes, in a cosmetically acceptable carrier, in relation to the total weight of the cosmetic agent, from about 0.01 to about 10 wt. % of at least one salt which includes at least one divalent cation, and / or of at least one salt which includes at least one trivalent cation.

The invention provides a method for preparing a beluga swimming bladder protein peptide. The method specifically comprises the following steps: treating and shredding fresh beluga swimming bladders, adding 5-fold dilute alkali liquor, soaking for 3 hours, performing centrifugal separation, washing the precipitate until the pH value is neutral; adding a 5-fold dilute acid solution, soaking for 2 hours, performing centrifugal separation, and washing the precipitate until the pH value is neutral; adding 10-fold water, extracting at the temperature of 90 DEG C for 6 hours, and filtering; cooling to 50 DEG C, regulating the pH value to 9, adding 0.5 percent of alkaline protease, performing enzymolysis at the temperature of 50 DEG C for 4 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; cooling to 45 DEG C, regulating the pH value to be 2.5, adding 0.3 percent of acid proteinase, performing enzymolysis at the temperature of 45 DEG C for 5 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; and regulating the pH value to be neutral, filtering, desalting, concentrating, and drying, thereby obtaining the beluga swimming bladder protein peptide.

There is provided herein a system and method for de-toxifying and de-scaling source water. In some embodiments, source water will be mixed with either an aluminum source or an iron source to separate endotoxins from acidic proteins and convert the naturally present bicarbonate in source water to carbon dioxide. Endotoxins and carbon dioxide will then be removed from source water by a stage of hydrophobic membranes to produce de-toxified and de-carbonated source water. Calcium hydroxide will be mixed with the de-toxified and de-carbonated source water to form precipitates comprising foulants and sulfate. A recoverable and reusable amine solvent will also be used to induce efficient precipitation. Possible reuse applications for the treated source water by the inventive methods that minimize excessive uses of potable water may include hydro-fracturing of shale and sand formations and heavy oil recovery by steam injection.

The invention belongs to the technical field of chromatography, and discloses an Fe3O4-COOH magnetic nano-material modified open tubular column as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing Fe3O4-COOH MNPs according to a hydrothermal method; (2) fixing PDDA on the inner wall of a capillary through electrostatic self-assembly; (3) adsorbing the Fe3O4-COOH MNPs to the surface of the PDDA through electrostatic self-assembly, so as to obtain the Fe3O4-COOH magnetic nano-material modified open tubular column. The Fe3O4-COOH magnetic nano-material modified open tubular column takes glycosylated magnetic particles as a chromatography stationary phase, has an excellent hydrophilic performance and electrostatic interaction due to rich carboxyl groups, and can be used for effectively inhibiting adsorption of a biological sample, performing excellent separation functions on acid protein, polypeptide, amino acid and the like, and effectively detecting the micro heterogeneity of glycosylated protein; the preparation method has the advantages that the conditions are mild, the operation is simple and convenient, and the repeatability and the reproducibility are high.

A low salt protein solution not capable of forming a gel is obtained from animal muscle tissue by forming an aqueous acidic protein solution which is filtered to remove salt and acid. The low salt protein solution can be formed into a gel by adding a physiologically acceptable salt to the low salt protein solution and heating the resultant protein solution with added salt.

The invention relates to the field of genetic engineering. In particular, the invention relates to a fungus-derived acid protease g412 as well as a gene and application thereof, and the amino acid sequence of the fungus-derived acid protease g412 is shown as SEQ ID NO.1 or SEQ ID NO.2. The acid protease disclosed by the invention has good properties, and can be applied to industries such as food, feed and pharmacy. According to the technical solution of the invention, a genetic engineering means can be utilized to produce the protease with excellent properties which is suitable for industrial application.

The invention provides an acidic Macadamia nut glycoprotein and a production method thereof. The production method mainly comprises the steps of adding water into defatted oil meal, carrying out boiling, adding an alkaline solution into precipitates to extract glycoprotein, separating acidic protein by an acidity gradient precipitation separating technology, and then, carrying out dewatering and drying, thereby obtaining the acidic Macadamia nut glycoprotein. According to the method, oil meal resources of Macadamia nuts are fully utilized, the production process is simple, the purification method is easy to operate, the product purity reaches up to 96% or more, the utilization ratio of resources is further increased, and waste discharge is also reduced. The invention further provides a glycoprotein product which has the bioactivity of resisting oxidation, propagating probiotics and the like. Simultaneously, the invention further provides a coproduction method of Macadamia nut proteins,neutral polysaccharides and acid glycoproteins. Three kinds of products, i.e., the Macadamia nut proteins, the neutral polysaccharides and the acid glycoproteins can be simply, conveniently and efficiently obtained from the defatted oil meal, the process is simple, production and popularization are facilitated, and thus, the method has a good application prospect.

The invention relates to the field of medicine, in particular to a preparation process of human immunoglobulin for intravenous injection. Octanoic acid is used for replacing ethanol as a precipitatingagent, so that acidic protein in blood plasma generates precipitates; IgG molecules with high isoelectric points are reserved on supernate; only through one-step precipitation reaction, the materialscan be transferred to a chromatography working procedure; caprylate is used; lipid envelope viruses are inactivated under the mild condition in short time; even partial non-lipid envelope viruses canbe inactivated; the action time is short; the effect is good; the bioactivity of the immunoglobulin cannot be easily influenced; the product safety is improved; the quality and the yield of the product are ensured; a two-step chromatography working procedure is used; most partial polymers and other impure protein are removed; the pyrogen is reduced; chromoprotein such as ceruloplasmin can be adsorbed; the product appearance is effectively improved; one-step precipitation reaction is used; the loss is reduced; the product yield is improved; the preparation process of human immunoglobulin for intravenous injection has the advantages of high product yield, high purity and high safety.

There is provided herein a system and method for de-toxifing and de-scaling source water. In some embodiments, source water will be mixed with either an aluminum source or an iron source to separate endotoxins from acidic proteins and convert the naturally present bicarbonate in source water to carbon dioxide. Endotoxins and carbon dioxide will then be removed from source water by a stage of hydrophobic membranes to produce de-toxified and de-carbonated source water. Magnesium hydroxide will be mixed with the de-toxified and de-carbonated source water to form precipitates comprising foulants and sulfate. A recoverable and reusable amine solvent will also be used to induce efficient precipitation. Possible reuse applications for the treated source water by the inventive methods that minimize excessive uses of potable water may include hydro-fracturing of shale and sand formations and heavy oil recovery by steam injection.

The present invention discloses separation and purification process of neurotoxin and cytotoxin of cobratoxin, and the process can separate neurotoxin and cytotoxin of cobratoxin simultaneously and raise the comprehensive utilization efficiency of cobratoxin. The process includes first treating cobratoxin liquid with acetone to eliminate liposoluble components from cobratoxin liquid, regulating the pH value of the water solution of cobratoxin and acetone powder with acid, centrifuging to eliminate the acid protein and polypeptide, specifically PAA adsorbing and precipitating the alkali protein and alkali polypeptide in cobratoxin liquid, centrifuging to separate PAA-alkali protein / polypeptide composition and eliminate water soluble components; desorbing PAA-alkali protein / polypeptide with pH variation, and eliminating PAA with calcium chloride; and separating and purifying neurotoxin and cytotoxin of cobratoxin with Sephadex G-50. The product has low salt content and may be frozen directly.

The invention belongs to the technical field of agro-biology and particularly relates to fungus-originated acid protease Bs2688 and gene and application thereof. An amino acid sequence of the acid protease is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The invention provides a new protease gene; proteinases with good character are produced by means of genetic engineering; the new protease gene is applicable to the industries, such as feed, food and medicine.

Hair treatment agents include selected protein hydrolysates and selected complexes of acidic protein hydrolysates and basic fatty acid amidoamines.

The invention provides acid protease Candidapepsin as well as a heterogeneous expression and purification method thereof. The acid protease Candidapepsin is sourced from candida tropicalis, an amino acid sequence of the acid protease Candidapepsin is as shown in SEQ ID NO.1, the enzyme activity of the acid protease Candidapepsin can be up to 268.2U / mL, and the specific enzyme activity can be up to2086.7U / mL and is far higher than the enzyme activity of like reported acid protease; the hydrolysis effect of the acid protease Candidapepsin is superior to that of commercial papain and commercialacid protease. The invention also provides the heterogeneous expression and purification method of the acid protease Candidapepsin. According to the heterogeneous expression and purification method provided by the invention, the yield of Candidapepsin protease is increased to a large extent, the yield can be up to 384.3mg / L, and a wide popularization and application prospect is obtained.

A meat or fish composition which retains moisture during cooking is provided. A dry protein mixture or an aqueous acidic protein solution derived from animal muscle tissue is added to the meat or fish prior to cooking. The dry protein mixture and aqueous acidic protein solution comprise myofibrillar proteins and sarcoplasmic proteins substantially free of myofibrils and sarcomeres.

The present invention relates to a compound enzyme preparation for pig feeds. The compound enzyme preparation is characterized by comprising the following raw materials in parts by weight: 22-27 partsof acid cellulase, 8-12 parts of glucanase, 23-27 parts of medium-temperature alpha-amylase, 23-27 parts of acid protease, 8-12 parts of saccharifying enzyme and 3-6 parts of lipase. The compound enzyme preparation for the pig feeds improves the digestion utilization rate of plant fibers and proteins, improves the utilization rate of the feeds, can effectively increase the immunity and digestionand absorption of pig bodies, reduces morbidity and improves ecological environment.

Hair treatment agents include selected amino acids and selected complexes of acidic protein hydrolysates and basic fatty acid amidoamines.

The invention belongs to the technical field of agrobiology, and particularly relates to a fungus-derived acid protease Bs2688 mutant and a gene and application thereof. The amino acid sequence of theprotease provided by the invention is shown in SEQ ID NO. 3. The invention provides a novel protease gene, and the protease with excellent properties is produced by using a genetic engineering meansand is applied to a feed, food, medicine and other industries.

The invention discloses a method for efficiently expressing acid protease and application thereof, and belongs to the field of gene engineering. The expression enzyme activity of the recombinant pichia pastoris P.pastoris GS115 / pPIC9K-en-alpha SP-acidprotea provided by the invention reaches 5395U / mL and is improved by 109% compared with the enzyme activity of a control strain containing original alpha-signal peptide; the acidic protein disclosed by the invention is in a pH range of 1-5, the enzyme can maintain the enzyme activity of 60% or more, the residual enzyme activity is 80% or more after the enzyme is treated at 20-40 DEG C for 30 minutes, and the acid protease can be suitable for industries such as food, feed or brewing and the like. According to the technical scheme provided by the invention, the acid protease suitable for industrial application can be produced by utilizing a genetic engineering means.

The present invention relates to a nano compound condom, Every condom contains a solid metal cation carrier material which is formed from (NaAg)2ZrPO4 or (NaNn)2ZrPO4 or (NaCu)2ZrPO4 or their mutually-combined product and whose microparticle size is below 0.1 micrometer and whose weight is 0.01-0.8 of that of emulsion. Its released metal cation can change the chemical composition of bacterial mechanism, and can break the acid protein on which the bacteria depend for existance, can kill AIDS vines and various VD pathogens, and can prevent cross-infection.