Method for preparing bone peptide extract and products thereby

An extract and bone peptide technology, applied in the field of biopharmaceuticals, can solve the problems of ineffective removal of allergic reactions, complicated process routes, and impact on product quality, and achieve the effects of ensuring clinical safety, reducing volume, and reducing operational difficulty

Inactive Publication Date: 2009-02-18
高翔
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The acid precipitation pH range of this preparation process cannot effectively remove acidic and neutral proteins, causing al

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] 1. Extraction: Put the crushed pork limb bones into the extraction tank, add purified water twice the weight of the feed, and extract by hot pressing, the pressure is controlled at 0.11-0.12mPa, and the hot pressing extracts twice, each time for 1 hour;

[0027] 2. Concentration: The medicinal solution obtained after hot-pressure extraction is concentrated, and the filtrate is first concentrated to 50%±2% of the weight of the raw material;

[0028] 3. Acid precipitation: Combine the concentrated solution again, remove the upper layer of grease, continue to concentrate to 60% of the weight of the raw material, and cool to room temperature; adjust the pH value to with 1:1 hydrochloric acid (prepared by volume ratio of purified water and 36-38% hydrochloric acid) 1.5, keep at 100℃ for 45 minutes, cool to room temperature, stand at 0-5℃ for more than 12h;

[0029] 4. Alkali precipitation: filter the acid precipitate, adjust the pH of the filtrate to 8.5 with ...

Example Embodiment

[0032] Example 2

[0033] 1. Extraction: Put the crushed pork limb bones into the extraction tank, add purified water twice the weight of the feed, and extract by hot pressing, the pressure is controlled at 0.11-0.12mPa, and the hot pressing extracts twice, each time for 1 hour;

[0034] 2. Concentration: The medicinal solution obtained after hot-pressure extraction is concentrated, and the filtrate is first concentrated to 50%±2% of the weight of the raw material;

[0035] 3. Acid precipitation: then combine the concentrated solution, remove the upper layer of oil, continue to concentrate to 70% of the weight of the raw material, cool to room temperature; adjust the pH value to with 1:1 hydrochloric acid (prepared by volume ratio of purified water and 36-38% hydrochloric acid) 3.0, keep at 100℃ for 45 minutes, cool to room temperature, stand at 0-5℃ for more than 12h;

[0036] 4. Alkali precipitation: filter the acid precipitation solution, adjust the pH value of the filtrate to ...

Example Embodiment

[0039] Example 3

[0040] 1. Extraction: Put the crushed pork limb bones into the extraction tank, add purified water 3 times the weight of the feed, hot-press extraction, pressure control at 0.11-0.12mPa, hot-press extraction twice, 1 hour each time;

[0041] 2. Concentration: The medicinal solution obtained after hot-press extraction is concentrated, and the filtrate is first concentrated to 50%±2% of the weight of the raw material;

[0042] 3. Acid precipitation: Combine the concentrated solution again, remove the upper layer of grease, continue to concentrate to 65% of the weight of the raw material, and cool to room temperature; adjust the pH value to with 1:1 hydrochloric acid (prepared with purified water and 36-38% hydrochloric acid by volume) 2.0, keep at 100℃ for 60 minutes, cool to room temperature, stand at 0-5℃ for more than 12h;

[0043] 4. Alkali precipitation: filter the acid precipitate, adjust the pH of the filtrate to 9.0 with 20% sodium hydroxide, keep it at 10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing bone peptide extract. The method comprises the following steps: (1) pig limb bones are smashed and added to water for hot-pressing extraction, and extract solution is concentrated so as to obtain concentrated solution on standby; (2) acid precipitation is carried out, which is to add dilute hydrochloric acid to the concentrated solution for acid precipitation; (3) alkali precipitation is carried out, which is to filter acid precipitation liquid and add lye to filtrate for alkali precipitation; (4) alcohol precipitation is carried out, which is to filter alkali precipitation liquid, add the dilute hydrochloric acid to the filtrate, reduce pressure, concentrate, cool, add ethanol in, keep stand for more than 12 hours at a temperature between 0 and 5 DEG C and obtain liquid medicine on standby; and (5) ultrafiltration is carried out, which is to ultrafilter the liquid medicine obtained in a step (4), collect ultrafiltrate and well mix the ultrafiltrate. The method has the advantages that the method extracts the pig limb bones and then carries out concentration, thereby providing convenience for production and reducing cost; the method respectively adopts acid precipitation, alkali precipitation and alcohol precipitation to remove acidic protein, basic protein and neutral protein, thereby speeding up ultrafiltration; the method adopts ultrafiltration to guarantee the thorough removal of impure protein and effectively control high molecular weight matter; and the method guarantees clinical safety.

Description

technical field [0001] The invention relates to a preparation method of bone peptide extract and a product prepared by the method, belonging to the field of biopharmaceuticals. Background technique [0002] The source of Guning injection is Guning injection. The preparation method of Guning injection is described in detail in the quality standard of Guning injection, and the preparation method of Guning injection is also described in "Biochemical Pharmacology" published by China Medical Press , Guning injection is made from pig limb bones, and phenol, talcum powder and activated carbon are added in the preparation method. [0003] At present, some extraction processes related to osteopeptide have been disclosed. Most of the processes only adopt the process of precipitating acidic protein and basic protein respectively with acid and alkali, and also use resin column adsorption to remove impurities. [0004] The Chinese patent application with the publication number CN 183100...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/32A61P19/08C07K2/00
Inventor 高翔
Owner 高翔
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap