39 results about "Limb bones" patented technology

The invention discloses a preparation method of a bone peptide solution and belongs to the field of biological engineering. The preparation method comprises the following steps: (1) crushing fresh limb bones of an animal, performing decompression extraction and ultrasonic treatment in a reaction kettle, extracting a concentrated solution and separating solid from liquid to obtain filtrate; (2) digesting by enzymolysis, namely respectively adding protease into the concentrated solution to perform enzymolysis; (3) heating the enzymolysis solution to realize enzyme deactivation, performing ultrafiltration and collecting ultrafiltrate to obtain the bone peptide solution. By crushing cells by adopting decompression extraction with assistance of ultrasonic wave, the extraction rate is increased; the method disclosed by the invention has the advantages of improving the degradation efficiency by adopting an enzymolysis method for production, reducing use of a great amount of acid, alkali and the like in a general extraction method, retaining active polypeptide in the bone peptide better and improving the product quality. The bone peptide product prepared by the method disclosed by the invention is good in quality, high in rate of finished products, remarkable in treatment effect, easy to operate and suitable for industrial production.

Disclosed is a dinosaur model with multi joint assembly that allows the user to grow creative idea and pursue self-lead learning during the assemblage of relatively many parts into the finished article and which is designed to allow free modification of actions and to provide a definite anatomical structure of dinosaur during the sequential assembly of a skeletal frame. It comprises: a skeletal frame in which skull, cervical vertebra, trunk skeleton, and tail bone units are sequentially connected through joints and the trunk skeleton unit is articulated with limb bone units; an integumental frame, responsible for appearance, comprising head integument, neck integument, trunk integument, tail integument, and limb integument units that are associated with the skull, the cervical vertebra, the trunk skeleton, the tail bone, and the limb bone units, respectively; and a connection member, intercalated between the skeletal frame and the integumental frame, for connecting the frames each other.

The invention provides a limb bone segmentation method and device, an electronic device and a readable storage medium, and relates to the field of medical image processing. The method comprises the steps of performing image preprocessing on a to-be-segmented limb DR image when the to-be-segmented limb DR image is obtained; obtaining a target limb DR image, using a first skeleton segmentation modelto carry out skeleton region identification and image segmentation on the target limb DR image; obtaining a skeleton distribution area of the target limb DR image; performing image cutting on the target limb DR image according to the bone distribution area; obtaining a limb foreground image corresponding to the bone distribution area; and finally, carrying out limb bone category identification and image segmentation on each bone part in the limb foreground image by adopting a second bone segmentation model to obtain a segmentation image comprising a limb bone category distribution region of each bone part, thereby finishing high-precision image segmentation operation and obtaining a limb bone segmentation result with high segmentation precision.

A method for setting up 3-D model of human limbs includes shooting image series of human limbs postures and picking up mark point from them, confirming space 3-D coordinates corresponded by these marks then obtaining space 3-D position data of limb bone at each time, solving out corresponding deformation parameter and volume by equation with coordinate value as well as deformation parameter of rotation conical curve, and using ball and curve to draw out model of human limbs.

A compound bone peptide injection for treating rheumatic and rheumatoid arthritides, bone fracture, osteoporosis, cervical spondylopathy is prepared from scorpion and the limb bone of young ox through high-temp and-pressure extracting, depositing in alcohol and then in acid, heat treating, cold storage, ultrafiltration, further preparing, pouring in containers in N2 atmosphere, sterilizing and lamp examining.

The invention relates to a puncture device, in particular to a four-limb bone tumor positioned puncture device. The puncture device comprises a ring, a circumferential rotating seat, a ring matching groove, an arc plate, a vertical slide post, a top screw, a threaded perforated plate, puncture screws and a screw seat. Four limbs can penetrate through the ring and fixed in any position of the ring,so that different positions of four limbs can be punctured. The ring matching groove is formed in the lower side of the circumferential rotating seat, the circumferential rotating seat is buckled onthe periphery of the ring through the ring matching groove, the vertical slide post is glidingly connected onto the circumferential rotating seat, upper and lower ends of the vertical slide post are fixedly connected with the threaded perforated plate and the arc plate, the upper side surface of the arc plate is an arc surface, the arc surface of the arc plate is attached to the inner circumference of the ring, the threaded perforated plate is connected with the top screw through threads, the lower end of the top screw props against the upper side of the circumferential rotating seat, the screw seat is arranged on one side of the circumferential rotating seat, and the puncture screws are inserted in the screw seat.

The invention discloses a method for improving the cell compatibility of bio-derived hydroxyapatite. The method comprises: after freezing pig limb bones for three months at a temperature of -176 to -18 DEG C, removing soft tissues from the surface of the bone tissue, sawing the bone into loose bone blocks by using a bone saw, carrying out ultrasonic cleaning with purified water without organic solvents, removing the bone marrow components, placing in a freeze-drying machine, and carrying out freeze-drying; placing in a supercritical carbon dioxide reaction kettle, carrying out a reaction, taking out, placing in an alkali solution, carrying out freeze-drying, taking out, placing in a ventilation muffle furnace, and carrying out gradual temperature increase and decrease treatment; and packaging, and carrying out cobalt-60 gamma ray radiation sterilization to prepare the bio-derived hydroxyapatite. According to the present invention, the degreasing and protein removing treatment is performed with supercritical carbon dioxide so as not to produce the organic matter residue, such that the biocompatibility and the biomechanical properties of the prepared material can be effectively improved.

The invention provides a limb bone movement simulation structure. A movable structure is connected between caput femur and pelvis or between scapula and humerus. The limb bone movement simulation structure is characterized in that the movable structure comprises an L-shaped plate, two perpendicular face plates of the L-shaped plate are respectively provided with shaft holes and shaft pins matched with the shaft holes. The shaft holes on one face of the L-shaped plate of a metal construction member are connected with the caput femur or the humerus through the shaft pins, and the shaft holes on the other face are connected with the pelvis or the scapula through the shaft pins to form the movable structure between the caput femur and the pelvis or between the scapula and the humerus and to form the simulation structure capable of simulating limb bone movements. The outer face portion of a bearing of the pelvis or the outer face portion of a bearing of the scapula is an arc face. A side portion of the other perpendicular face plate of the L-shaped plate is provided with a limiting boss which is movably matched with the arc face to control rotation angle between the femur and the pelvis or between the scapula and the humerus, and to simulate limitation of limb movements of a human body. The limb bone movement simulation structure is exquisite in structure, vivid in appearance, economic, applicable, and capable of well achieving the purposes of teaching and training.

The method for diagnosing osteoporosis, using a method for determining the dynamics of closure of cavities to evaluate the effectiveness of the application of various bone protectors, relates to the field of medicine, for the diagnosis of osteoporosis and measurement of the effectiveness of therapeutic and prophylactic treatment of conditions associated with osteoporosis. The method is characterised by laboratory, histological and radiological methods of examination (standard radiography) and by measuring bone mineral density using the radiological absorptive method on osteometers, into which an additional diagnostic criterion is introduced, namely the measurement of cavities by specially adjusting them to measure cavities in trabecular bone sections using the radiological absorptive method and in addition, distilled water is introduced into special incubation apparatuses around the limb bones of the hands and feet which are being examined. The problem being addressed by the claimed invention is to objectively evaluate the effectiveness of a preparation (preparations) (bone protector) and measure cavities in trabecular bone sections. It proposes to improve the diagnostic capabilities of computerised osteometers by measuring cavities caused by osteoporosis and determine the severity of the disease not only on the basis of mineral density, but also on the basis of the presence of cavities in trabecular bone sections.

The invention discloses a preparation technology for a bone peptide glucose injection. The injection is prepared from adding a certain proportion of glucose on the basis of a bone peptide raw materialliquid; the bone peptide raw material liquid can be prepared from performing hot pressing extraction, concentration, alcohol precipitation, acid-base precipitation and ultrafiltration on limb bones of pigs; and adding the glucose in proportion, and diluting the mixture with injection water so that the injection can be prepared. The injection consists of the bone peptide raw material liquid, the glucose and the injection water; and each 100 ml of the injection contains 50-100 mg of the bone peptide raw material liquid (by polypeptide) and 5 g of the glucose. Thus, a process that a bone peptideinjection is temporarily dissolved in a glucose solution during intravenous drip in the past can be simplified, convenient clinical use can be realized, efficiency can be enhanced, and the probability of drugs being polluted can be reduced; and the injection can be used for promoting fracture healing.

The invention belongs to the technical field of specimen preparation, and particularly relates to a method for removing soft tissues from a canine bone specimen. The method comprises the steps: removing large muscles attached to a skeleton, and dividing the skeleton into a skull, a spine, limb bones, a sternum and ribs; preparing alkaline protease solutions with the concentrations of 0.3% and 0.5%; putting the skull, the limb bones and the chest ribs into 0.3% alkaline protease solution, and respectively soaking in a constant-temperature incubator at 50 DEG C for 36 hours, 66 hours and 60 hours; putting the spine bone into a 0.5% alkaline protease solution, and soaking in a 50 DEG C constant-temperature incubator for 60 hours; and taking out the bone and washing with water. After the softtissues are removed by the method, the prepared specimen is complete in bone and less in residual grease of the bone, and the prepared specimen is white in bone color.

The invention relates to a method for efficiently separating and culturing mesenchymal stem cells of a primarily-cultured rabbit, which comprises the specific steps of: executing an experimental animal by pumping air embolism and then soaking for 15-20min with alcohol for sterilizing, placing in a clean bench for separating limb bones, shearing two ends of the bones after repeatedly washing with a PBS (Phosphate Buffer) and DMEM (Dulbecco's Modified Eagle Medium) culture solution, and washing marrow cavities with the DMEM solution containing heparin with concentration of 625U/ml; respectively filtering marrow liquids obtained from the step 1 by using syringes with different diameters, preparing a monoplast suspension and then slowly injecting into an eccentric pipe containing isovolumetric Percol 1 for centrifuging for 20min, collecting an intermediate cloud cell layer, washing cells with an LG-DMEM solution, centrifuging for 5min, repeatedly washing for 2-3 times, adding the washed cells to the LG-DMEM solution containing a proper proportion of double antibodies (1%) and comprising 10% fetal calf serum, blowing and beating to form a cell suspension, counting and regulating the cell density to 1*10<8>/L, inoculating in a 25cm<2> plastic culture bottle, and placing in a culturing box with conditions of a temperature of 37 DEG C and a humidity of 5 percent for culturing. The invention can be effectively, conveniently and efficiently separating and culturing the mesenchymal stem cells of the primarily-cultured rabbit and is prepared for further basic experiments and clinical research.

The invention discloses an elasticity modulus determination method and preparation method of an individualized limb bone fracture setting internal fixation device.The elasticity modulus determination method comprises the steps that CT scanning is performed on a target limb bone, a three-dimensional model is rebuilt, the material attributes are given to the target limb bone, a finite element analysis model is built, and an elasticity modulus gradient distribution rule is obtained through analysis; the internal fixation device is drawn according to the operation requirements, and the elasticity modulus of the internal fixation device is obtained by aiming at the assembly relationship between the fracture part of the limb bone and the internal fixation device, combining the elasticity modulus gradient distribution rule and adopting an interpolation method.According to the elasticity modulus determination method and preparation method of the individualized limb bone fracture setting internal fixation device, internal individualization and external individualization are both achieved, the internal fixation device is externally fit with the human body limb bone and internally good in matching with the biomechanical characteristic of the individual bone, stress distribution of a steel plate and screws is more uniform and more reasonable, the stress shielding effect of an internal fixation material on the bone can be reduced, the compressing force of a fracture interface can be increased, the fracture heating fixation requirement is better met, and a scientific basis can be supplied to following research and development of a novel material, optimized design of an operation method and operative instruments and building of a relative database.

The invention discloses an externally-applied traditional Chinese medicine emulsion for the prevention and treatment of knee osteoarthritis and a preparation method thereof. The externally-applied traditional Chinese medicine emulsion for the prevention and treatment of knee osteoarthritis is an O/W type dispersion, the average particle size of a dispersion phase is 0.5 micron, and the externally-applied traditional Chinese medicine emulsion is pharmaceutically named as a microemulsion preparation or a sub-nano emulsion preparation; and the externally-applied traditional Chinese medicine emulsion for the prevention and treatment of knee osteoarthritis is prepared from the following raw materials in parts by mass: 0.42 part of eucommia barks, 0.07 part of sangusis draconis, 0.25 part of fructus psoraleae, 0.07 part of buthus martensi karsch, 0.7 part of rhizoma drynariae, and 0.7 part of rehmanniae vaporata, and the content of Chinese medicinal materials in the finally prepared emulsion is 0.22%. The emulsion disclosed by the invention has the effects of nourishing liver and kidney, strengthening muscles and bones, and promoting blood circulation to remove meridian obstruction, and is suitable for treating orthopedic diseases such as limb bone arthralgia, neck, waist and leg pains and the like caused by the deficiency of liver and kidney and the stasis of tendons and vessels; and the emulsion can be applied to affected parts directly through the skin, and has a characteristic of remarkable drug effect.