Method for improving cell compatibility of bio-derived hydroxyapatite

A hydroxyapatite and compatibility technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of loss of cytocompatibility, affecting the speed of osteogenesis, transplantation failure, etc., to improve biocompatibility , improve cell compatibility, improve the effect of impact

Active Publication Date: 2017-12-19
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Bone transplantation is the second largest tissue transplant after blood transfusion. Currently, the commonly used bone graft materials mainly include bio-derived materials and artificial synthetic materials. Biologically-derived materials have a more similar physical structure and chemical structure to autologous bone tissue. The composition is widely used in clinic, but bone tissue derived from allogeneic or heterogeneous bone tissue has strong antigenicity, and it is easy to cause immune rejection after transplantation, resulting in transplantation failure. In order to effectively reduce the immunogenicity of such materials, often Decellularization, deproteinization and other methods are used to remove the antigen carrier, and the most commonly used method of deantigenization for xenograft bone is calcined bone prepared by high temperature calcination to remove collagen and other organic components in bone tissue, the main component of which is hydroxyl Apatite, this kind of bio-derived hydroxyapatite effectively reduces the antigenicity, but it loses the good cytocompatibility of the original bone tissue, which is not conducive to the attachment of osteoblasts after implantation, thus affecting the osteogenesis At the same time, it needs to use a large amount of organic solvent to remove protein during the preparation process, which is easy to affect the microstructure of bone tissue and cause organic solvent residue, which affects the biocompatibility of the material

Method used

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  • Method for improving cell compatibility of bio-derived hydroxyapatite
  • Method for improving cell compatibility of bio-derived hydroxyapatite
  • Method for improving cell compatibility of bio-derived hydroxyapatite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Observation of supercritical carbon dioxide degreasing effect, scanning electron microscope observation of bone materials that have undergone supercritical carbon dioxide degreasing and non-degreasing bone materials. , Fat is removed thoroughly, and cell components can be removed at the same time.

[0031] Depend on figure 1 It can be seen that there are a large number of fat droplets in the material without degreasing, and the fat cleaning is not thorough; figure 2 It can be seen that there are no fat particles on the surface of supercritical carbon dioxide degreased bone tissue, and cell lacuna after cell removal can be seen.

Embodiment 2

[0033] The biologically derived hydroxyapatite-like material prepared by the present invention was observed with the naked eye and a scanning electron microscope, and the results showed that the color of the material without supercritical carbon dioxide degreasing was yellowish-white after calcining, indicating that the fat removal in the early stage was not complete, and the subsequent hydroxyapatite The preparation of the material has a significant impact; the color of the calcined material degreased by supercritical carbon dioxide is ivory white, and the observation of the microstructure shows that the traditional calcined method is prone to produce a large number of microcracks on the surface of the calcined bone, while the calcined bone prepared by this method has basically no cracks on the surface. microcracks occur.

Embodiment 3

[0034] Example 3 In order to show the biomechanical properties of the bio-derived hydroxyapatite-like material prepared by the present invention, the biomechanical properties of hydroxyapatite prepared by traditional methods and hydroxyapatite prepared by this method were measured, as follows:

[0035] The prepared composite material was trimmed into 5×5×5mm cubes, 5 pieces in each group, and its compressive strength was measured by RGT-20A microcomputer-controlled universal testing machine, the loading rate was 1mm / s, and the elastic modulus was calculated. The specific results are shown in Table 1. It shows that the hydroxyapatite prepared by this method can effectively improve the biomechanical properties of the material due to the reduction of microcracks generated during calcination.

[0036] Table 1 Compressive strength and elastic modulus of bio-derived hydroxyapatite-like materials prepared by traditional calcination method and this method

[0037]

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Abstract

The invention discloses a method for improving the cell compatibility of bio-derived hydroxyapatite. The method comprises: after freezing pig limb bones for three months at a temperature of -176 to -18 DEG C, removing soft tissues from the surface of the bone tissue, sawing the bone into loose bone blocks by using a bone saw, carrying out ultrasonic cleaning with purified water without organic solvents, removing the bone marrow components, placing in a freeze-drying machine, and carrying out freeze-drying; placing in a supercritical carbon dioxide reaction kettle, carrying out a reaction, taking out, placing in an alkali solution, carrying out freeze-drying, taking out, placing in a ventilation muffle furnace, and carrying out gradual temperature increase and decrease treatment; and packaging, and carrying out cobalt-60 gamma ray radiation sterilization to prepare the bio-derived hydroxyapatite. According to the present invention, the degreasing and protein removing treatment is performed with supercritical carbon dioxide so as not to produce the organic matter residue, such that the biocompatibility and the biomechanical properties of the prepared material can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of biological transplantation materials, and in particular relates to a method for improving the cell compatibility of biologically derived hydroxyapatite. Background technique [0002] Bone transplantation is the second largest tissue transplant after blood transfusion. Currently, the commonly used bone graft materials mainly include bio-derived materials and artificial synthetic materials. Biologically-derived materials have a more similar physical structure and chemical structure to autologous bone tissue. The composition is widely used in clinic, but bone tissue derived from allogeneic or heterogeneous bone tissue has strong antigenicity, and it is easy to cause immune rejection after transplantation, resulting in transplantation failure. In order to effectively reduce the immunogenicity of such materials, often Decellularization, deproteinization and other methods are used to remove the antigen carrier,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/50
CPCA61L27/12A61L27/3608A61L27/365A61L27/3691A61L27/50A61L2430/02
Inventor 张育敏牛晓军
Owner SHANXI UNIV OF CHINESE MEDICINE
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