Novel canine bone specimen manufacturing method

Abstract

The invention belongs to the technical field of specimen preparation, and particularly relates to a method for removing soft tissues from a canine bone specimen. The method comprises the steps: removing large muscles attached to a skeleton, and dividing the skeleton into a skull, a spine, limb bones, a sternum and ribs; preparing alkaline protease solutions with the concentrations of 0.3% and 0.5%; putting the skull, the limb bones and the chest ribs into 0.3% alkaline protease solution, and respectively soaking in a constant-temperature incubator at 50 DEG C for 36 hours, 66 hours and 60 hours; putting the spine bone into a 0.5% alkaline protease solution, and soaking in a 50 DEG C constant-temperature incubator for 60 hours; and taking out the bone and washing with water. After the softtissues are removed by the method, the prepared specimen is complete in bone and less in residual grease of the bone, and the prepared specimen is white in bone color.

Description

technical field [0001] The invention belongs to the technical field of specimen preparation, and in particular relates to a method for preparing a novel dog bone specimen. Background technique [0002] The steps of making canine bone specimens mainly include removing soft tissue, degreasing, bleaching, and imitating the normal and natural posture of animals for stringing. Among them, the removal of soft tissue is an important step. In the process of removing soft tissue, it is easy to cause bone damage, and the residual oil will cause the bone to turn black in subsequent production. The degree of soft tissue removal directly affects the subsequent degreasing time, bleaching effect and Skeletal integrity is the key to making high-quality canine bone specimens. Because the bone size and bone density of different species are different, the methods for removing soft tissue are also different. At present, the most commonly used method is to use low-concentration sodium hydroxide...

AI Technical Summary

Problems solved by technology

Because the bone size and bone density of different species are different, the methods for removing soft tissue are also different. At present, the most commonly used method is to use low-concentration sodium hydroxide to remove soft tissue and degrease, and sodium hydroxide solution is corrosive. Bones are prone to damage and require constant observation. Failure to master the concentration and time will cause irreversible damage to bone specimens. Improper operation will also cause damage to human skin. Therefore, it is necessary to improve the method of removing soft tissues from dog bone specimens. On the basis of improvement, perfect the degreasing and bleaching procedures to obtain a complete white bone

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