248 results about "Specimen preparation" patented technology

A bio electron microscope and an observation method which can observe a bio specimen by low damage and high contrast to perform high-accuracy image analysis, and conduct high-throughput specimen preparation. 1) A specimen is observed at an accelerating voltage 1.2 to 4.2 times a critical electron accelerating voltage possible to transmit a specimen obtained under predetermined conditions. 2) An electron energy filter of small and simplified construction is provided between the specimen and an electron detector for imaging by the electron beam in a specified energy region of the electron beams transmitting the specimen. 3) Similarity between an observed image such as virus or protein in the specimen and a reference image such as known virus or protein is subjected to quantitative analysis by image processing. 4) A preparation protocol of the bio specimen is made into a chip using an MEMS technique, which is then mounted on a specimen stage part of an electron microscope to conduct specimen introduction, preparation and transfer onto a specimen holder.

Provided is a specimen preparation apparatus capable of supplying a specimen from the specimen preparation apparatus to a specimen analyzer without burdening an operator. This specimen preparation apparatus comprises a stained specimen preparation part for preparing a specimen on a slide glass and staining the specimen, a keeping part for storing the stained specimen slide glass prepared in the stained specimen preparation part and a control part for deciding whether to supply the stained specimen slide glass to the keeping part or to the external apparatus.

A specimen carrier for use with an ex-situ lift-out (EXLO) milling process includes a carrier top surface having at least one specimen support area and at least one aperture formed through the specimen carrier top surface. The aperture includes a first opening having an open wider upper end and a narrower lower end. The first opening is bounded by opposed sidewalls in spaced-apart orientation that are inwardly inclined from the wider upper end to the narrower lower end. The aperture is configured to enable a specimen to sit over the opening and can be wedged between the first opening opposed sidewalls so that a region of interest to be milled is centered about the open end of the opening. Specimens so mounted can then be re-thinned via charged particle instruments such as focused ion beam (FIB) milling, broad beam ion milling, or via laser ablation.

The invention of Integrated Versatile and Systems Preparation of Specimens relates an open module technology which integrates synchronously the methods of protection, isolation and alteration of specimens into the “One for All” product or kit. The product or kit comprises a core module without or with Plug-in modules and a set of comprehensive protocols which can simultaneously or individually isolate systems biomolecules including DNA / ccfDNA, Large RNA / mRNA / ccfRNA, Small RNA / miRNA / ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite versatilely from a vast variety of specimens including solid specimens and liquid specimens. The product or kit can accept new and custom Plug-in modules to expand functions and applications. The product or kit prepares specimens and biomolecules with features and benefits of high quality, easy, fast, no toxicity, safe to user and environment, low demanding, cost-effective, reducing waste, saving nature resources and protecting environment, and leads to a low-carbon and Green economy in preparation of specimens.

There is disclosed a cross-sectional specimen preparation apparatus. The milling position can be modified or corrected in a short time. Also, the internal structure of the specimen can be known. The apparatus has an optical observation device for observing a cross section of the specimen milled by the ion beam. During ion beam irradiation or when the irradiation is interrupted, a shutter is opened. The cross section of the specimen can be observed while maintaining the vacuum inside a processing chamber. The apparatus further includes an adjusting mechanism for varying the relative position between the specimen and the shielding material. Whenever one sectioning operation ends, an image of the cross section is accepted and the milling position is moved an incremental distance. A three-dimensional image of the specimen is constructed from obtained plural images.

In an apparatus for microwave-assisted preparation of specimens, the microwave chamber for reception of specimens to be processed is embodied as a waveguide (3), in particular as a monomode waveguide, and is equipped with at least one opening (3a) for introduction of the at least one specimen (11) into the waveguide. A cooling circuit comprises a cooling means (8) adapted to cool the fluid (12), which fluid surrounds the at least one specimen and is separated from the cooling liquid of the cooling circuit, in the region of the at least one specimen (11) inside the waveguide. The opening (3a) can be sealed in microwave-tight fashion, by means of a closure means (7), during operation of the apparatus.

A blood analyzer comprising: a specimen preparation section for preparing a measurement specimen that is used to measure a white blood cell count among CBC measurement items; a measurement section for obtaining optical information from blood cells contained in the measurement specimen; and a controller carrying out operations comprising: classifying blood cells contained in the measurement specimen into at least white blood cells and blood cells suspected to be abnormal blood cells based on the optical information; obtaining distribution data of the white blood cells and distribution data of the blood cells suspected to be abnormal blood cells; counting the white blood cells based on the distribution data of the white blood cells; and determining presence or absence of the abnormal blood cells in the blood sample, based on the distribution data of the blood cells suspected to be abnormal blood cells. A blood analyzing method and a computer program product are also disclosed.

The invention relates to an electrolytic extraction and determination method for nonmetallic inclusions in steel, and belongs to the field of physical research methods of metal. Aiming at the defects of an existing electrolytic method for observing the nonmetallic inclusions in the steel, and by improvement and innovation from the aspects such as specimen preparation, electrolyte, an electrolyzer and an extraction method, the invention provides a method, comprising the steps of for dissolving a specimen in the specially-made electrolyzer by adopting novel inorganic electrolyte with a simple formula, carrying out filtering, magnetic separation and sampling, and then directly observing the nonmetallic inclusions in the steel on filter paper; the method has the advantages of accurate determination and wide application range.

The invention discloses an adjustable model for the preparation of a rock specimen containing cracks and a use method thereof. The form comprises a base, a size-adjustable model frame and an upper press plate; the upper press plate is a rectangular steel plate which can be taken away or positioned on the model frame like a lid; the center of the rectangular steel plate is provided with a round hole, the circumference of the round hole is provided with angle markings, a round positioning plate is located in the round hole, the round positioning plate is provided with clearance positioning grooves of different distances and angle indication marks. For the model frame size is adjustable, therefore suitable to the preparation of specimens of various sizes, thus overcomes the old day shortage of one model set for only one specimen size, and achieves the function of specimens preparation in many different sizes; because the round positioning plate can spin at will, therefore can adjust the angle of a prefabricated clearance. The clearance positioning groove on the round positioning plate can adjust the distance between the clearances, this fact blazes a trail in the shortcomings of monotone styles of prefabricated model clearances in the past and achieves the prefabrication of clearances with different angles and different distances.

The invention relates to a transmission electron microscope specimen, which comprises a substrate on which an interest region is arranged on. A dielectric layer and a metal layer are sequentially formed on the substrate and the interest region. The difference between the contrast of the dielectric layer and the contrast of the interest region is larger than a preset value. The preparation method of the transmission electron microscope specimen comprises the steps of: providing the substrate and forming the interest region on the substrate; forming the dielectric layer on the interest region and the substrate; forming the metal layer on the dielectric layer, wherein the difference between the contrast of the dielectric layer and the contrast of the interest region is larger than the preset value; and sectioning the transmission electron microscope specimen, wherein the transmission electron microscope specimen comprises the interest region, the dielectric layer and the metal layer. The preparation method of the transmission electron microscope specimen is simple and feasible and clearer and more accurate transmission electron images can be obtained by using the transmission electron microscope specimen.

The invention provides a method for displaying 6082 aluminium alloy structure, pertaining to the technical field of metallographic specimen preparation. The specific steps of the method are: a 6082 aluminium alloy test specimen is put on a waterproof abrasive paper for coarse grinding and polishing, then anode oxidation is carried out, the formulation of anode oxidation solution is 45 to 70mL of hydrofluoric acid and 12 to 16g of boronic acid, then distilled water is added for dilution to be 1L; the voltage of anode oxidation is 20 to 40V, the time of anode oxidation is 70 to 120s, then the test specimen is washed and blow-dried; and the clear 6082 aluminium alloy structure can be observed under a polarizing microscope. The invention has the advantage that the problem that 6082 aluminium alloy structure is difficult to display is solved, the structure of aluminium alloy structure after recrystallization and extrusion is clearly displayed. The method is simple and convenient to be implemented.

An apparatus for in-situ specimen preparation is described. The apparatus includes an ion beam column 21 including at least: an liquid metal alloy ion source 56 including a first element for providing a light ion species with a mass of 10 g / mol to 60 g / mol and a second element for providing a heavy ions species with a mass of 150 g / mol or higher, a mass separator 58 for selectively separating the light ion species and the heavy ion species, and a focusing element for focusing the ion beam on a specimen. The apparatus further includes a specimen-beam-tilt unit for tilting the ion beam with respect to the specimen.

A method of preparing ultra-thin TEM specimens is provided by flipping the sample upside down for FIB thinning Such preparation method is compatible with the ex-situ lift-out system and offers high quality TEM specimens without the curtaining effect.

The invention discloses a specimen preparation and organization exposure method of metallic phase of zinc and alloy thereof. The method comprises the following steps: performing wet grinding with a flat file with fine teeth, performing mechanical polishing, performing chemical polishing and exposing organization, wherein the chemical polishing step adopts the chemical polishing liquid composed of sulfuric acid, lactic acid, hydrogen peroxide and water; and the organization exposure step adopts the etching agent composed of potassium metabisulfite, sodium sulfate, chromium oxide and water, thus samples for metallographic observation can be successfully prepared. The operation is simple and convenient, the time for sample preparation is short, the consumption of material is low; and the organization exposure definition of the prepared samples is high, thus the method is particularly suitable for the specimen preparation and organization exposure of metallic phase of zinc and alloy thereof.

The invention discloses a preparation method for a transmission electron microscope (TEM) specimen of a cadmium zinc tellurium (CdZnTe) and metal interface. The preparation method is used for solving the technical problem that the reject rate is high because the specimen is broken very easily when the TEM specimen of the CdZnTe and metal interface is prepared in the prior art. The technical scheme is that the preparation method comprises the following steps of: after a CdZnTe single crystal is linearly cut, washed and polished, conducting chemical corrosion; preparing a metal electrode in a resistive evaporation coating machine; folding and sticking the specimen and drying the specimen on a heating table; and grinding, polishing and thinning the specimen along the normal line direction ofa sticking gap, polishing the two surfaces of the specimen, sticking a copper grid on one surface, using a manual grinder to thin the specimen, and finally using a Gatan 691 ion mill to thin the specimen till the specimen is punctured. Since a multi-piece sticking method and a manual grinder for grinding and polishing through high-grade abrasive paper are adopted, after thinning and polishing arecompleted, the damage caused to an interface area in the thinning process is minimized and the success rate of specimen preparation is improved. The success rate of specimen preparation is improved from 30-40 percent in the background technology to 60-70 percent.

The analyzer is composed of optical system, signal acquisition system and computer. Design of integrating sphere is utilized in the optical system of the analyzer. The procedure of the signal acquisition system is as follows. Incident light controlled by computer passes through optical modulator becomes varied light alternated intensity in high and low. Continuously, with being passed through grating spectrum analyzer, the light irradiates on a sample to be tested. Near infrared reflected back from the sample passing through the integrating sphere irradiates on a sensor so as to measure out the intensity of the light. Processing the measured intensity, computer obtains each component of sample measured and contents of each component. The portable analyzer can test rock and mine directly without need of specimen preparation. The measured data can be obtained in 70 second. Thus, it is suitable for use in field.

A specimen kit having a tiny chamber is disclosed for a specimen preparation for TEM. The space height of the chamber is far smaller than dimensions of blood cells and therefore is adapted to sort nanoparticles from the blood cells. The specimen prepared under this invention is suitable for TEM observation over a true distribution status of nanoparticles in blood. The extremely tiny space height in Z direction eliminates the possibility of aggregation of the nanoparticles and / or agglomeration in Z direction during drying; therefore, a specimen prepared under this invention is suitable for TEM observation over the dispersion and / or agglomeration of nanoparticles in a blood.

The invention discloses a voltage-transformation type softening dripping performance testing method and equipment. The voltage-transformation type softening dripping performance testing method comprises the following steps of test specimen preparation, sample loading, program temperature rise control, gas ingredient control, pressure control and data collection. The voltage-transformation type softening dripping performance testing equipment comprises a pressurization device, a molten drop furnace body, a control system, a supporting frame, a gas supply system and a collection device, whereinone side of the supporting frame is provided with the molten drop furnace body; the bottom end of the molten drop furnace body is provided with the collection device connected with the bottom of the supporting frame; and the control system and the gas supply system are connected through a pipeline. By use of the equipment, load pressure applied to furnace charges can be changed, and in an experiment process, the load pressure applied to the furnace charges gradually changes along with the rise of the temperature. Blast furnace charge softening dripping performance parameters obtained by the method and the equipment can more objectively reflect the internal practical situation of the blast furnace.

The invention discloses a metallographic specimen clamping device which comprises an upper clamp and a lower clamp. The upper clamp and the lower clamp are each provided with at least two through holes. The through holes in the upper clamp correspond to the through holes in the lower clamp one to one. Bolts penetrate through the through holes in the corresponding positions of the upper clamp and the lower clamp, and nuts are further arranged on the bolts. Anti-skid protruding structures are arranged on the opposite clamping faces of the upper clamp and the lower clamp. According to the metallographic specimen clamping device, specimen grinding time is saved, the work efficiency of specimen preparation is improved, and the labor intensity of workers is lowered.

The invention discloses a uniformly mixing and filtering integrated processing mechanism for a specimen. A filter ventilation mechanism is driven to move vertically and rotate horizontally by a motor; the filter ventilation mechanism is rotated and is pressed down; the top pressing surface of the filter ventilation mechanism presses a specimen container; a ventilation needle is inserted into the specimen container for ventilating; a uniformly mixing mechanism is arranged below the filter ventilation mechanism and is driven to rotate forwardly or reversely by a uniformly-mixing motor; the specimen container is accommodated in a hollow uniformly-mixing seat and is driven to rotate forwardly or reversely by the uniformly-mixing motor; a test tube carrying mechanism is arranged below the uniformly-mixing mechanism, and is provided with an accommodating hole for accommodating a test tube; a jacking mechanism is arranged below the uniformly-mixing mechanism, and is driven to move vertically by a jacking motor; when the jacking mechanism moves upwards, a platform is jacked to press against the bottom of the test tube, so that the test tube is butted with the bottom of the specimen container, and a specimen preparation in the specimen container is dripped into the test tube. The uniformly-mixing and filtering integrated processing mechanism for the specimen is simple in structure, and integrates a uniformly-mixing function and a filtering function.

The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.

The present invention enables a sample to be observed in a clean state directly after preparation of a final observation surface when preparing a sample for observing a material that is sensitive to heat. The present invention is a method of preparing a sample using a charged particle beam device including a microprobe having a cooling mechanism, a first sample holder having a mechanism for retaining a sample in a cooled state, and a stage into which the microprobe and the first sample holder can be introduced, the method including cutting a bulk-shaped sample piece from the sample on the first sample holder retained in a cooled state; adhering the sample piece to a distal end of the microprobe that is cooled to a fixed temperature and transferring the sample piece to a second sample holder for thin film observation retained in a cooled state, which is different from the first sample holder, within a vacuum chamber of the charged particle beam device; separating the sample piece that has been transferred to the second sample holder from the microprobe and thin film processing the sample piece to a thickness that is less than the thickness during cutting; and observing the sample piece after the thin film processing.

The invention discloses a preparation method of colored metallographic specimens of aluminum alloys of Al-Mg and Al-Mg-Si series. By the steps of electrochemical polishing and anode lamination, gas evolution amount can be increased, bubbles are convenient to desorb, the fluidity and the flowing speed of solution are increased, and bubbles adsorbed on the end surface of a specimen can be effectively discharged, so that the electric field applied to the end surface of the specimen is more uniform, and the solution flows at a high speed. The colored metallographic specimens of aluminum alloys can effectively display microscopic structures of different aluminum alloy series, the grain size can be conveniently measured, and the problem that the aluminum alloy grains are difficult to display in the traditional specimen preparation method is solved. The specimens prepared by the preparation method has the advantages of high contrast display, clear grain boundary, and high accuracy of the measurement result when used for color metallographic analysis.

The invention discloses a preparation method of macrofungi specimens. According to the preparation method, a macrofungi specimen is cleaned and undergoes anticorrosion pretreatment; then, the specimen is plasticized with polyethylene glycol; a plasticizer on the surface of the plasticized specimen is sucked dry with filter paper; and finally, prepared transparent urea-formaldehyde resin and the plasticized macrofungi specimen are fused completely. By the preparation method, breeding of microbes in the macrofungi specimen is inhibited effectively, the original color of the macrofungi specimen is maintained effectively, and the problem of environmental pollution during preservation of specimens is solved. The preparation method provided by the invention is suitable for preservation of macrofungi of various colors as well as preservation of macrofungi specimens and opens up a new way for the preparation of macrofungi specimens.

A sample vial is disclosed for use in conjunction with an automated cytological specimen preparation system.

The invention discloses a rock triaxial compression test method suitable for sample preparation through drilling. The method comprises the steps of 1, excavating a test adit in a mountain body by a blasting method; 2, specimen preparation: selecting a cylinder as a specimen, wherein the diameter D of the specimen is more than or equal to 30cm, and the height H of the specimen is equal to the product of the diameter D and (2.0-2.5); 3, sheathing a ring-shaped rubber capsule on the peripheral surface of the specimen, and sealing and anchoring a ring-shaped groove by a ring-shaped steel plate, wherein the shape of the ring-shaped rubber capsule is matched with those of the specimen and the ring-shaped groove; 4, installing axial loading equipment on the top surface of the specimen, finally, installing a plurality of displacement sensors in a symmetrical way on a bearing plate, and therefore, a rock triaxial compression test can be carried out according to the requirements of technical regulations. The rock triaxial compression test method has the advantages that the specimen can be prepared on site in a mechanized way, and the problems that the rock is manually dug in the existing specimen preparation process so that the long time is consumed, the excavated volume is large, and the cost is high can be solved. After the rock triaxial compression test method is used, the time of the rock triaxial compression test is greatly shortened, the working efficiency is increased, and the test expense is reduced.

The invention discloses a preparation method for a titanium alloy rocking-rolling piece metallographic specimen. A titanium alloy TC4 rocking-rolling piece is taken as a raw material; the preparationmethod comprises the following steps of dividing, performing coarse grinding, performing fine grinding, performing electrolytic polishing, corroding by using a corrosive and the like in sequence. Thepreparation method for the titanium alloy rocking-rolling piece metallographic specimen disclosed by the invention can be applied to a product which deforms easily, is large in deformation amount, high in stress and irregular in appearance; after the specimen is corroded, the specimen is in a matte colour and bright colour; the corrosion uniformity is good; a corroding method has high selectivity;important metallographic information, such as grain morphology and grain structure type, can be obtained by observing the corroded metallographic structure through a metallographic microscope, so that the corroded metallographic structure is more beneficial to observation and is observed more accurately; the preparation method is low in cost, simple in specimen preparation process, short in specimen preparation period, and worthy of market promotion and application.

The invention discloses a demounted combination-type metallographic specimen high-efficient grinding and polishing clamp. The demounted combination-type metallographic specimen high-efficient grinding and polishing clamp is characterized in that one end of each of four connection rods (2) is inserted into a corresponding peripheral hole in a square-shaped central connection block (1), and the other ends of the four connection rods (2) are separately connected with a magnetic suction disc (7), so that the clamp of a cross-shaped integral structure is formed; holes for placing metallographic specimen clamping components are formed in each connection rod (2); during a specimen grinding and polishing process, well-clamped metallographic specimen clamping components are placed in the holes, and are fixed through screws (5) in the metallographic specimen clamping components. By using the demounted combination-type metallographic specimen high-efficient grinding and polishing clamp, metallographic specimens do not need to be grabbed by hand directly during a metallographic specimen preparation process, so that the problem that the specimen preparation quality is not stable enough when the specimens are held by hand and the like are solved.

The invention relates to an encapsulating method for a biological specimen. The technical proposal adopted is that: a biological specimen is fixed, sterilized, antisepticised and cleaned, polyoxyethylene (PEG six hundred) is used to plasticize the specimen, the plasticized specimen is placed in a porcelain dish which is provided with absorbent paper, is then put into an incubator of thirty degrees to forty degrees for twelve to twenty-four hours and are finally fully integrated with a prepared transparent Lauxite until the preparation of specimen is completed. The encapsulating method has the advantages of a good encapsulating effect, mould proofing, insect prevention and color retention, which is particularly applicable to encapsulating the biological specimen with more water content, such as Craspedacusta, earthworms, mollusks and small-sized fishes.

The invention discloses a fluorescence in situ hybridization method of 5S rDNA on a plant chromosome. The fluorescence in situ hybridization method mainly comprises the steps of chromosome specimen preparation, 5S rDNA probe preparation, chromosome fluorescence in situ hybridization, hybridization signal detection and the like. The invention mainly solves the problem that the labeling of the conventional fluorescence in situ hybridization probe is complicated and cumbersome and provides the simple, convenient, fast and accurate probe labeling and hybridization method. According to the fluorescence in situ hybridization method, the FISH positioning of the 5S rDNA on the plant chromosome can be completed within three hours. The 5S rDNA probe is an oligonucleotide probe. When the 5S rDNA probe is synthesized, fluorophore can be added into the 5S rDNA probe to modify the 5S rDNA probe, so that labeling is not needed. Compared with the prior art, the fluorescence in situ hybridization method is simple and low in cost.