The present invention provides an ELISA percolation method for rapidly detecting pathogen antibody, a kit for detection, and a preparation method of the kit. According to the present invention, an antigen-antibody reaction is used to make antigen or second antibody be immobilized on a nitrocellulose membrane, the pathogen IgM (IgG) antibody in a serum sample and the corresponding solid-phase antigen or second antibody on the membrane produce specific binding so as to form a complex when the serum sample passes through the nitrocellulose membrane due to a percolation effect while other unrelated substances are filtered, enzyme-labeled antibody or antigen is added and is combined with the antigen-antibody complex on the membrane during the filtering, and a coloration liquid is added to carry out coloration so as to produce the purple blot conveniently observed by naked eyes; and the time consuming of the detection process is short, the sensitivity is high, the accuracy is strong, the shelf life of the kit can achieve more than or equal to 12 months with the stable dilution buffer solution and the coloration prepared through the method, and the whole detection has characteristics of simple and rapid operation process, no requirement of special equipment, high sensitivity, and high accuracy.