34 results about "Brucella abortus" patented technology

The technology is a veterinary pharmaceutical formulation of two vaccines, one from an RNA viral vector system constituted by an RNA recombinant particle that codifies for a Cu/Zn superoxide dismutase protein of Brucella abortus, and the other based on naked RNA constituted by a recombinant molecule of naked RNA that carries a sequence for the synthesis of at least one recombinant Cu/Zn superoxide dismutase protein of Brucella abortus and some Semliki Forest virus genes. An expression system based on the Semliki Forest virus and a use of this system, in addition to a method for the preparation of the pharmaceutical formulations.

According to the invention, through amplifying a strong promoter in Ensifer adhaerens and carrying out bioinformatics analysis and functional verification, a strong promoter is obtained, and the strong promoter can be widely used for gene expression, genetic manipulation and strain improvement in Sinorhizobium meliloti, Zymomonas mobilis, Caulobacter crescentus, Pseudomonas denitrificans, Agrobacterium tumefaciens, Brucella abortus, Pseudomonas fluorescens, Rhizobium leguminosarum, Ensifer adhaerens and other alpha-proteobacteria and has a nucleotide sequence of SEQ ID NO: 1. The invention also relates to a plasmid vector containing the strong promoter, a method for constructing a genetic engineering strain by using the strong promoter, and an application of the corresponding strain and ahost cell in starting expression of a target gene.

A PCR kit for simultaneously detecting Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis as well as a preparation method and a using method thereof, and belongs to the field of gene detection of anthropozoonosis pathogens. The PCR kit comprises a PCR liquid reactant, DNA polymerase, positive quality control standard substances and negative quality control standard substances; the PCR liquid reactant comprises four pairs of primers for identifying Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis and a PCR buffer solution containing dNTPs, Mg<2+> and double distilled water. The PCR kit has high practicability, can be used for directly detecting the type of Brucella in a contaminated sample, has high detection speed, high sensitivity (10<2>cfu/ml), high specificity and good repeatability, is safe, reliable, quick, simple and convenient, can be used for qualitative detection of the type of Brucella in the sample, and can replace traditional etiological and serological methods.

Methods and compositions for the treatment of Brucella induced diseases and disorders are disclosed herein. In preferred embodiments, the invention relates to vaccines. In additional embodiments, the invention relates to formulations capable of releasing said vaccines at a controlled rate of release in vivo. In further embodiments, the invention relates to modified strains of the bacteria Brucella melitensis and Brucella abortus. In still further embodiments, the invention relates to compositions that do not induce clinical symptoms or splenomegaly in a subject receiving said compositions.

The invention discloses a mycobacterium bovis and brucella abortus dual detection card and a preparation method thereof and aims to provide a detection card which is capable of simultaneously detecting mycobacterium bovis and brucella abortus in a bovine serum specimen, is short in detection time, high in stability, simple in operation and intuitive and reliable in result judgment and does not need other instruments or professional personnel. According to the technical key points, the detection card comprises a shell (1), wherein a sample adding hole (2) and an observation window (3) are formed in the shell (1); and a colloidal gold test strip is arranged in the shell (1). The detection card is characterized in that the colloidal gold test strip comprises a bottom plate (4); a sample pad (5), a gold label pad (6), a coating membrane (7) and an absorbent pad (8) are sequentially connected onto the bottom plate (4); a mycobacterium bovis membrane protein MPB70-MPB83 detection line T1, a brucella abortus LPS detection line T2 and a goat anti-rabbit polyclonal antibody quality control line C are formed in the coating membrane (7); and the three lines are arranged in parallel. The detection card belongs to the technical field of biology.

Compositions and methods for the diagnosis and prevention of B. abortus infection are provided.

The invention discloses a method for detecting a coupling antibody of brucella abortus and luminescent quantum dots, which belongs to the field of detector and quarantine. The method comprises the following steps: (1) quantum dots, brucella abortus polyclonal antibodies and coupling agent are mixed and act for two hours in room temperature to obtain solution containing quantum dots which are coupled with brucella abortus polyclonal antibodies; (2) the coupling product is purified, and unreacted brucella abortus antibodies and quantum dots are removed; (3) the coupling product acts with a test sample coated on a slide, the luminescent detection is then carried out after the dissociative coupling product is rinsed out, and the test sample contains corresponding antigens when the luminescence exists. The invention has the advantages that the method has extremely low detection limit, high sensitivity and strong specificity, and simultaneously, a plurality of brucella abortus antigen ingredients can be detected rapidly, simply and conveniently; in addition, the device is simple and the cost is low and the method can be used for on-site rapid detection of brucella abortus.

The invention relates to a Brucella abortus reference serum bank. The Brucella abortus reference serum bank has the following meanings: (1) the Brucella abortus reference serum bank with a certain size is established for the first time internationally; (2) reference substances are provided for scientific assessment of a brucellosis diagnostic method; (3) substance basis is provided for development of various brucellosis diagnostic methods for cattle.

The invention provides primer sets and kits for the multiple RPA detection of brucella abortus, brucella melitensis and brucella suis, and belongs to the technical field of brucella detection. The primer sets include B107F, B107R, B192F, B192R, B285F and B285R; and the nucleotide sequences of the B107F, B107R, B192F, B192R, B285F and B285R are successively shown as SEQ ID No. 1- SEQ ID No. 6. Multiple RPA detection can be performed on samples through the specific primer sets, the detection sensitivity of the brucella abortus is 10 pg/[mu]L, and the detection sensitivities of the brucella melitensis and brucella suis are 1 pg/[mu]L; and the provided primer sets and kits can respectively detect three spiked samples which are manually set serum, beef and milk, and compared with a brucella Bruce-Ladder detection method of national standard GB/T 18646-2018 diagnostic techniques for animal brucellosis, results are consistent.

The invention discloses a strong promoter from ensifer adhaerens as well as a plasmid vector and an application of the strong promoter. Function verification is performed by amplifying one strong promoter in ensifer adhaerens, and the strong promoter which can be widely applied to gene expression, gene operation and strain improvement of alpha-proteobacteria such as sinorhizobium meliloti, zymomonas mobilis, caulobacter crescentus, pseudomonas denitrificans, agrobacterium tumefaciens, brucella abortus, pseudomonas fluorescens, rhizobium leguminosarum and ensifer adhaerens is obtained and has the nucleotide sequence being SEQ ID NO:1. The invention also relates to the plasmid vector containing the strong promoter, a method for constructing a gene engineering strain by use of the promoter, the corresponding strain and an application of a host cell in starting target gene expression.

The invention discloses a xylose-induced promoter. By amplifying a xylose-induced promoter in sinorhizobium meliloti, bioinformatics analysis and function verification are carried out, the xylose-induced promoter which can be widely applied to gene expression, genetic gene operation and strain improvement in alpha-proteobacteria such as Sinorhizobium meliloti, Zymomonas mobilis, Caulobacter crescentus, Pseudomonas denitrificans, Agrobacterium tumefaciens, Brucella abortus, Pseudomonas fluorescens, Rhizobium leguminosarum and Sinorhizobium adhaerens is obtained, and the nucleotide sequence of the promoter is SEQ ID NO: 1 or SEQ ID NO: 2. The invention also relates to a plasmid vector containing the xylose-induced promoter, a method for constructing a genetic engineering strain by using thepromoter, a corresponding strain, and application of a host cell to induction and starting of expression of a target gene under xylose inducible conditions.

The invention provides a method for detecting a brucella abortus attenuated vaccine strain S19 and a primer group used for detection, wherein the sequence of the adopted primer group is SEQ ID NO: 1-4. The primer group and method have advantages that 1) the primer group and method are efficient and sensitive, amplification can be completed in 30 min, and the lowest detection limit of an amplification template is 1.0*10<-3> ng/[mu]l; 2) the primer group and method are high in specificity, and two pairs of PRA primer groups specifically identify and detect S19 and don't cause any cross reactionwith brucellin of other species and biological types, brucellin vaccine strains and common non-brucellin strains; 3) the primer group and method are simple to operate, the prepared RPA reaction systemundergoes a reaction in a 39-degree constant-temperature condition to undergo a reaction, and a PCR instrument is not required; and 4) the primer group and method are high in flux, a large amount ofsamples can be detected in one time in a water bath or a constant-temperature incubator, and are not limited by the quantity of test holes of the PCR instrument.

The invention discloses brucella abortus virus fluorescent microsphere immunochromatography test paper and a preparation method and detection method thereof. The invention aims to provide the brucellaabortus virus fluorescent microsphere immunochromatography test paper which has strong specificity, high sensitivity and good repeatability and can be used for quantitative and qualitative analysis.According to the technical scheme, the brucella abortus virus fluorescent microsphere immunochromatography test paper comprises a bottom plate, the bottom plate is sequentially connected with a samplepad, a combination pad, a chromatographic membrane and a water absorption pad, a line C and a line T are arranged on the chromatographic membrane, and the combination pad is sprayed with fluorescentmicrospheres labeled with a BrG11 antibody; a BrF11 antibody is sprayed on the line T; and the line C is painted with A goat anti-mouse IgG antibody. The invention belongs to the field of pathogen detection of experimental animals.

This invention discloses methods for identifying Francisella tularensis vaccine candidates. It enables identification of novel vaccine candidates and quality assurance for vaccine batches, assessment of protection in vaccinates and identification of the infecting agent in vaccinates. Mice were first vaccinated with Brucella abortus O-polysaccharide (OPS) vaccine. These animals were then given 10 LD₅₀s of F. tularensis live vaccine strain (LVS). Sixty percent (60%) of the vaccinated mice survived the multiple lethal doses. Sera were collected from these surviving mice and the antibodies were used to probe supernatant and cell lysates of live F. tularensis LVS cultures. Several F. tularensis components were identified only by the noted “survivor” antisera. Of these identified proteins, enzyme digestions and chemical oxidation suggest post-translational modifications of some proteins e.g. a 52 kDa glycoprotein, a 45 kDa lipoprotein and a 19 kDa nucleoprotein. The 52 kDa component caused nitrous oxide induction in tissue cultures at low concentrations, cell death at high concentrations. Vaccination with this gave partial protection while addition of other components acted synergistically to give enhanced protection from 250 LD₅₀s of F. tularensis LVS.

The invention discloses an establishment method of indirect ELISA (enzyme-linked immunosorbent assay) for detection of Brucella abortus HSP70. The establishment method includes the steps of preparinga fusion protein of Brucella abortus HSP70, and purifying the fusion protein to obtain a purified protein; using the purified protein as an antigen to detect a Brucella serum sample, and determining antigen coating concentration, serum dilution, confining liquid type, secondary antibody diluted concentration and color rendering time; determining a critical value of indirect ELISA; determining an ELISA results judging standard; analyzing sensitivity and specificity of an indirect ELISA method. HSP70 is subjected to prokaryotic expression to obtain the purified protein; the purified protein is used as the antigen to establish the indirect ELISA method for detection of Brucella abortus; the important method is provided for the serological test of brucellosis.

The invention discloses a primer-probe for RNA-isothermal-amplification detection on brucella abortus, a kit and a detection method. The specific detection primer-probe, the detection kit containing the detection primer-probe and the detection method employing RNA isothermal amplification by utilizing the detection kit have the characteristics of high sensitivity, high specificity, low pollution (an amplification product RNA is easy to degrade in a natural environment) and rapidness, have low requirements on instruments and are simple in operation process.