The invention provides a detection method for diagnosing
Brucella abortus by using a recombinant overlapping
peptide, and relates to the technical field of
veterinary drugs and detection of
zoonosis. The detection method for diagnosing the
Brucella abortus by using the recombinant overlapping
peptide (ROP-OMP31) comprises the following steps: screening an
antigen protein, namely screening the
antigen protein OMP31 from
Brucella abortus outer mold proteins; vaccine design, namely designing ROP-OMP31 by using a recombinant overlapping
peptide technology; artificially synthesizing, namely constructing a vector, performing
prokaryotic expression and performing HPLC identification; performing
escherichia coli transformation and
protein purification; performing
activity detection on ROP-OMP31, namely feeding the mouse for 3 weeks after nasal drip, and carrying out
staining to detect the activity by using acid-fast
staining; performing a
guinea pig skin test, establishing a
guinea pig model, and performing the
skin test after feeding for 45 days; and performing a bovine
skin test, establishing a bovine
brucellosis model, and performing out the
skin test. According to the present invention, the OMP31-ROP design comprises more
antigen T cell epitopes; the
protein detection purity is higher, and the corresponding quality is better; and meanwhile, compared with a PCR (
Polymerase Chain Reaction) technology and an Elisa, the method is easy to operate,
safer and lower in cost.