Brucella abortus fluorescent quantum point coupling antibody detection method

Abstract

The invention discloses a method for detecting a coupling antibody of brucella abortus and luminescent quantum dots, which belongs to the field of detector and quarantine. The method comprises the following steps: (1) quantum dots, brucella abortus polyclonal antibodies and coupling agent are mixed and act for two hours in room temperature to obtain solution containing quantum dots which are coupled with brucella abortus polyclonal antibodies; (2) the coupling product is purified, and unreacted brucella abortus antibodies and quantum dots are removed; (3) the coupling product acts with a test sample coated on a slide, the luminescent detection is then carried out after the dissociative coupling product is rinsed out, and the test sample contains corresponding antigens when the luminescence exists. The invention has the advantages that the method has extremely low detection limit, high sensitivity and strong specificity, and simultaneously, a plurality of brucella abortus antigen ingredients can be detected rapidly, simply and conveniently; in addition, the device is simple and the cost is low and the method can be used for on-site rapid detection of brucella abortus.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows scientists to study how different types of particles behave differently when they come into contact or interacting with each other. It also includes methods that improve upon these interactions such as modifying certain chemicals during manufacturing processes or adding special agents like chelating reagents onto nanoparticles made from them. These improvements make it possible to create highly sensitive probes called Quantum Dot (QDS). By combining QDL's unique properties together, this new type of particle becomes more effective at identifying small organisms even if their size exceeds 10 nm.

Problems solved by technology

This patents describes various technical methods for detecting harmful bacteria such as Escherichia coli (EGC) by combining certain types of small particles called quantum dots with other organism components like DNA or protein. These techniques have been developed over time due to its ability to specifically target disease causing agents within an environment without affecting human health. However, current methods require expensive equipment and complicated procedures which limit their practicality across multiple industries where they can potentially benefit.

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