Brucella abortus reference serum bank

A technology of bovine Brucella and Brucella, which is applied in the field of bovine brucellosis reference serum bank, can solve the problems of interfering with guinea pig complement, difficulty in universal use, and difficulty in the preparation of hemolysin

Inactive Publication Date: 2017-05-31
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to prepare the hemolysin required for the complement fixation test, the test operation is cumbersome, and it is difficult to be widel

Method used

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  • Brucella abortus reference serum bank
  • Brucella abortus reference serum bank
  • Brucella abortus reference serum bank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] ——Preparation of clinical positive reference serum

[0033]1. Collect more than 300 samples of serum from dairy cows with clinical history of abortion and not immunized with vaccines, and use tiger bengal plate agglutination test for preliminary determination. Take 0.03ml of the serum to be tested and the same amount of Brucella tiger red plate agglutination test antigen (batch number: 201501) for plate agglutination, and observe the reaction result within 4 minutes. Results A total of 79 positive sera were detected by the tiger bengal test.

[0034] 2. For the 79 samples that were tested positive for tiger bengal, the antibody titer of Brucella was further determined by test tube agglutination test, and the serum samples with antibody titer above 1:100 were reserved for further complement fixation test. The specific operation is as follows: the test tube agglutinated antigen (batch 201501) was diluted 1:20 with 0.5% phenol saline (abbreviation: stone saline); the seru...

Embodiment 2

[0047] ——The results of 79 sera measured by three different detection methods.

[0048] The 79 bovine sera that were initially screened positive by tiger bengal were further tested by test tube agglutination test and complement fixation test, and the sera that were tested positive by all three methods were used as positive reference serum samples for Brucella bovis, and were accurately packaged lyophilized. The detection results of 79 serums by three different methods are as follows (Table 4):

[0049] Table 4 The results of clinically collected Brucella bovis positive serum using three different detection methods

[0050]

[0051]

[0052] Note: 1. RBT: tiger bengal plate agglutination test, take 0.03ml of the serum to be tested and the same amount of Brucella tiger bengal plate agglutination test antigen (batch number: 201501) for plate agglutination, and observe the reaction result within 4 minutes; + +++: There are large agglutinated particles or particles, and the...

Embodiment 3

[0061] ——Preparation of vaccine immune serum

[0062] Track and monitor the antibody level of immune flocks, and collect serum as the source of immune positive serum samples. Adopt brucellosis vaccine (S2 strain) to carry out oral immunization to test sheep and goat, 2.5 billion CFU / head portion. After the completion of immunization, the antibody level was monitored weekly by tiger bengal plate agglutination test, and part of the bovine serum was collected when the antibody level was high, and titer determination and complement fixation test were confirmed according to the preparation method of clinical positive serum samples. Each serum 0.5ml / cartridge was aliquoted and freeze-dried.

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Abstract

The invention relates to a Brucella abortus reference serum bank. The Brucella abortus reference serum bank has the following meanings: (1) the Brucella abortus reference serum bank with a certain size is established for the first time internationally; (2) reference substances are provided for scientific assessment of a brucellosis diagnostic method; (3) substance basis is provided for development of various brucellosis diagnostic methods for cattle.

Description

technical field [0001] The invention relates to a bovine brucellosis reference serum bank, which belongs to the technical field of biological products. Background technique [0002] Brucellosis (Brucellosis, referred to as brucellosis) is a zoonotic disease characterized by abortion and fever caused by Brucella or Brucella, which seriously threatens humans and various animals. life and health. This disease not only has serious harm to the reproduction and production performance of animals, but more importantly, people are often difficult to cure after being infected with Brucella, thus causing serious public health problems. Elimination of brucellosis has therefore been one of the most important goals of public health programs in countries where Brucella is endemic. [0003] Brucellosis has existed in the world for a long time. Human beings have gradually deepened their understanding of the disease, and the diagnostic methods for brucellosis have been continuously improved...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/569
CPCG01N33/56911G01N33/96G01N2333/23
Inventor 丁家波蒋卉朱良全彭小薇冯宇张磊
Owner CHINA INST OF VETERINARY DRUG CONTROL
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