29 results about "Guinea pig" patented technology

The invention discloses an application of a compound fresh bamboo juice in a guinea pig asthma model, and relates to the field of medicines, a Hartley albino guinea pig is adopted to construct an OVA induced asthma model, and the model is verified to be successfully constructed through evaluation indexes; evaluating the efficacy of the successfully constructed guinea pig asthma model by using different doses of compound fresh bamboo juice, and evaluating the efficacy of the guinea pig asthma model by scoring guinea pig capsaicin stimulation cough inducing times, alveolar lavage fluid leukocyte number, inflammatory factor content, lung tissue hydroxyproline content and behavioral signs. And evaluating the treatment effect of the compound fresh bamboo juice through Western blot detection, lung histopathology and immunofluorescence staining indexes. In a guinea pig asthma model, high-dose and medium-dose groups of the compound fresh bamboo juice can be used for remarkably reducing the number of cough, reducing the number of white blood cells in alveolar lavage fluid and the level of inflammatory factors and inhibiting pulmonary fibrosis, and a good treatment effect is shown. The results provide powerful support for potential application of the compound fresh bamboo juice in asthma treatment.

The invention discloses an application and a preparation of a miR-125b-5p inhibitor or antagonist for treating and / or preventing myopia. According to the application, a miR-125b-5p inhibitor or antagonist, particularly miR-125b-5p antiagomir with a nucleotide sequence as shown in SEQ ID NO: 8, is used for inhibiting the expression of miR-125b-5p in sclera and increasing the expression of a target gene tp53, so that the transdifferentiation of sclera fibroblasts can be remarkably reduced, sclera remodeling can be reduced, and the diopter reduction and ocular axis extension of guinea pig deprivation myopia can be retarded; the composition can be used for preventing myopia and controlling myopia progress, has the advantages of low administration frequency and high safety, and provides a safer, more effective and more convenient prevention and control means for myopia patients, especially teenagers.

This invention relates to a guinea pig model of chronic obstructive pulmonary disease (COPD) with chronic bronchitis, its preparation method, and its application. Specifically, this invention provides a guinea pig model of COPD with chronic bronchitis, established by subjecting guinea pigs to at least two intratracheal infusions of lipopolysaccharide (LPS) combined with repeated exposure to cigarette smoke. This results in the guinea pigs exhibiting pathophysiological characteristics consistent with those of COPD with chronic bronchitis in terms of cough sensitivity, airway inflammation, airway hypersecretion, lung function, and histopathology. This lays a solid foundation for in-depth research and drug screening of COPD with chronic bronchitis.

The invention discloses a pharmacodynamic test method for a cough relieving mixture, which comprises the following steps: constructing four core pharmacodynamic test sub-models containing ammonia water to cause young mouse cough, citric acid to cause young guinea pig cough, rabbit in-vitro trachea cilia mucus movement and young mouse trachea phenol red excretion and a body model of a test result analysis sub-model; each pharmacodynamic test sub-model is provided with a plurality of groups of test arrays and test individuals; pathogen data is imported to generate a simulated disease scene, and a corresponding drug sequence is called to intervene and collect physiological index change data; and multi-dimensional pharmacodynamic parameters are evaluated through the analysis sub-model, and a dose-effect relation curve and an evaluation report are generated. According to the invention, a parallel verification system for antitussive and expectorant tests is formed, the antitussive effective dose, the expectorant effective dose and the effective dose range under the dual action of the medicine are determined through multi-model cross validation, the accuracy degree of judging the effectiveness of the medicine is greatly improved, and a reliable method support is provided for evaluating the efficacy of the cough-stopping mixture.

The application provides a preparation method of Artemisia sphaerocephala pollen allergen and an Artemisia sphaerocephala pollen allergen injection, and belongs to the technical field of pollen allergen vaccine preparation. After the Artemisia sphaerocephala pollen is defatted and dried, the Artemisia sphaerocephala pollen is extracted by using an extraction solution, the supernatant is filled into a dialysis bag to be dialyzed, concentrated, enzymolyzed and desalted, and then chromatography is carried out, and polypeptides with molecular weights of 23-46KD and 67-82KD are collected to be the Artemisia sphaerocephala pollen allergen. The Artemisia sphaerocephala pollen allergen injection comprises the Artemisia sphaerocephala pollen allergen, sodium chloride, PBS buffer, phenol and thiourea. The Artemisia sphaerocephala pollen allergen injection of the application fills the blank of Artemisia sphaerocephala pollen allergen injection, can effectively reduce the allergic degree of guinea pigs to Artemisia sphaerocephala pollen, and can be stably stored at 2-8 DEG C.

The invention belongs to the technical field of animal model construction, and particularly relates to a spinal cord hypertension syndrome disease model modeling method and application. A guinea pig or a macaque is used as an experimental animal, and a composite biostimulation material and an accurate pressure regulation and control modeling system are combined to realize modeling. The composite biostimulation material is composed of a core pressure slow-release layer, a middle biocompatible coating and a surface targeting binding layer, has excellent biocompatibility, degradability and targeting property, and can reduce non-specific inflammatory response; the precise pressure regulation and control modeling system comprises a pressure sensing module, a micro-injection module, a real-time monitoring module and a main control module, and can dynamically regulate and control the pressure in the spinal cord in a closed-loop mode. The modeling method is easy and convenient to operate and good in repeatability, the model building success rate exceeds 95%, the pathological features are highly similar to clinical features and can last for 4 weeks, a reliable experimental model is provided for mechanism research, drug screening and therapeutic schedule evaluation of the spinal cord high pressure syndrome, and the modeling method is suitable for large-scale popularization.

The utility model belongs to the field of experimental animal equipment, and particularly relates to a guinea pig fixing frame which comprises a bottom plate, an operation table is arranged above the bottom plate, an adjusting frame is arranged between the bottom plate and the operation table, and the adjusting frame can change the inclination angle of the operation table and the height of the operation table. A plurality of adjusting holes are formed in the operating table and used for fixing four limbs of a guinea pig, and an elastic belt is further arranged on the operating table; the adjusting frame comprises two first supports and two second supports, the two first supports are oppositely arranged, the two second supports are oppositely arranged, the tops of the first supports are fixed to the bottom of the operation table, the bottoms of the first supports are fixed to the bottom plate, a hinge support is arranged between the two first supports, and the hinge support comprises a connecting rod and a sliding rod. One end of the connecting rod is connected to the first support, the other end of the connecting rod is connected with the end of the sliding rod, and the connecting rod is connected with the first support through a screw. The guinea pig fixing device can safely and stably fix a guinea pig, and experiment operators are prevented from being scratched by the guinea pig.

This invention relates to the field of microbial technology, specifically disclosing a thermoresistant Aeromonas verrucosa phage, its screening method, and its applications. The phage is deposited under CGMCC No. 47009. This phage belongs to the Myocaudidae family and exhibits significant heat resistance, maintaining a titer of 10 even after treatment at 70°C for 60 minutes. ^ 8 This bacteriophage exhibits a PFU / mL concentration above [amount missing] and can withstand the high temperatures of feed pelleting. It displays broad-spectrum lytic activity against *Aeromonas vesiculosus* and can cross-lyze *Aeromonas guinea pig* and *Aeromonas hydrophila*. This invention also provides a rapid screening method for this heat-resistant bacteriophage. Through a cycle of instantaneous heat treatment at 70°C followed by rapid cooling in an ice bath, genetically stable bacteriophages can be obtained within 5 days, solving the problems of long cycles and poor stability associated with traditional acclimatization methods. The bacteriophage and composition of this invention can be used to prepare veterinary drugs and disinfectants for the prevention and treatment of aquatic aeromonas diseases, showing broad application prospects.

The invention relates to a type II herpes simplex virus strain HSV-2 / KM-1 and application thereof, and belongs to the technical field of virus microorganisms. The II-type herpes simplex virus strain HSV-2 / KM-1 is preserved in the China Center for Type Culture Collection on July 25, 2025, and the preservation number of the II-type herpes simplex virus strain HSV-2 / KM-1 is CCTCC (China Center for Type Culture Collection) NO: V202550. The II-type herpes simplex virus strain HSV-2 / KM-1 disclosed by the invention has cell tropism and tends to infect genital epithelial cells; the II-type herpes simplex virus strain HSV-2 / KM-1 is applied to neutralizing antibody detection of mouse serum, and the instrumental effect of the II-type herpes simplex virus strain HSV-2 / KM-1 in immunogenicity evaluation of vaccines is disclosed. According to the invention, the II-type herpes simplex virus strain HSV-2 / KM-1 is simultaneously utilized to successfully construct a Hartley guinea pig genital herpes model or a BALB / c mouse genital herpes model, and the models can be used as new tools for HSV-2 vaccine evaluation.

The invention discloses a nucleic acid preparation containing diketo dioxins, and belongs to the technical field of biological medicine. The technical problem to be solved is to improve the skin penetration absorption effect of the transdermal absorption promoting preparation and reduce the allergy and irritation. According to the technical scheme, the nucleic acid preparation is composed of an siRNA solution and a lipid solution. The siRNA solution contains siRNA (small interfering Ribonucleic Acid); the lipid solution comprises cationic lipid and a penetration enhancer; the penetration enhancer is a diketo dioxane compound, and has a structural formula as shown in a formula I in the specification. Experiments show that siRNA of a pharmaceutical composition containing the nucleic acid preparation, poloxamer, a thickening agent, a pH regulator and purified water is high in cumulative permeation amount, and a guinea pig Buehler test shows that the preparation is free of anaphylaxis and can effectively promote wound healing of diabetic mice, so that the nucleic acid preparation has large-scale production potential and value of rapid conversion into clinical application.

A pet nail filer device is disclosed, which is a nail rounder and smoother that can be applied to dogs, cats, rabbits, guineapigs, birds and other pet animals, etc., as needed. The pet nail filer device comprises a base component that is configured in a small, ergonomic, rounded cuboidal shape that is sized and shaped to be held in a palm of a user's hand with a hinged lid component. An interchangeable nail file component is releasably secured to the base component. The interchangeable nail file component is shaped like a small cup to fit an individual pet nail. The interchangeable nail file component comprises sandpaper like grit material within the cup cavity to safely file away the pet's nail. The interchangeable nail file component comes in a range of sizes and working mechanisms, ensuring that pet owners and groomers can find the perfect fit for their current animal.

This invention relates to the field of microbial technology, specifically to a thermoresistant Aeromonas guinea pig bacteriophage, its composition, and its applications. The bacteriophage was deposited on June 14, 2024, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 45946, and named RKP-AE24003. The composition comprises the aforementioned bacteriophage. The bacteriophage can be used to prepare products that kill or inhibit Aeromonas guinea pig; and products for the prevention and / or treatment of aquatic diseases caused by Aeromonas guinea pig. This bacteriophage RKP-AE24003 exhibits good thermoresistance and strong genetic stability, allowing for stable storage in high-temperature environments, significantly improving the stability of industrial production and its applicability to high-temperature scenarios.

The invention relates to the field of plant extracts, in particular to application of a hawthorn extract in preparation of a medicine for treating gall-stone. Dry hawthorn pulp is smashed, heated and extracted with an ethanol solution, filtered, concentrated and dried to obtain the hawthorn extract, the hawthorn extract can inhibit and treat gall-stone, the content of total bile acid (TBA) in a guinea pig gall-stone model is increased, the content of Ca < 2 + > and TBIL (total bilirubin) is reduced, and all indexes return to the normal level. In addition, the composite extract prepared by the invention and the combination of the composite extract and the hawthorn extract can effectively inhibit and treat gall-stone.

The application relates to a guinea pig head frame structure for astigmatism research and a mounting operation method and a preparation method thereof. Firstly, three-dimensional anatomical data of the head and neck of a guinea pig is acquired; a three-dimensional model of a head frame and a lens support is designed based on the data, and is formed by using an additive manufacturing process. The guinea pig head frame structure comprises the head frame and the lens support, the head frame is provided with a mouth and nose opening and a neck U-shaped ring; the lens support is provided with a lens clamping ring and is connected with the head frame. The head frame is sleeved on the head and neck of the guinea pig and is fixed by using a rope; an astigmatism experiment lens is pressed into the lens clamping ring; the experiment lens can be disassembled and eye medicine can be applied through a light transmission hole in a state of maintaining the fixation of the head frame. The application aims to overcome the technical problems that a current animal model molding device fixing mode in astigmatism experimental research is traumatic, complicated in operation, poor in stability and difficult to cooperate with eye medicine.

The utility model relates to the technical field of guinea pig breeding, and provides a simple guinea pig breeding cage which comprises a cage body, the cage body is composed of an upper cage part and a lower cage part in a detachable mode, the upper cage part is used for containing guinea pigs, the lower cage part is used for containing padding, a grid plate is arranged in the upper cage part, and the grid plate is arranged in the lower cage part. A material supporting plate capable of ascending and descending is arranged in the lower cage part; a padding layer is formed at the bottom of the lower cage part by lifting padding to a certain height through the retainer plate, when the padding is replaced, the stripper plate moves downwards firstly, the dirty padding moves downwards along with the stripper plate under the action of gravity and falls into the lower cage part, then the upper cage part and the lower cage part are split, guinea pigs stay in the upper cage part, then the dirty padding in the lower cage part is dumped and cleaned, and the guinea pigs are prevented from being damaged. When the guinea pig padding is replaced, new padding is laid, then the upper cage part and the lower cage part are spliced again, the material supporting plate moves upwards, the material supporting plate moves upwards to drive the padding to enter the bottom of the upper cage part to form a padding layer again, padding replacement is completed, operation is easy, and the guinea pig padding replacement efficiency is greatly improved.

The invention belongs to the technical field of biology, and particularly discloses an anti-aeromonas cainea egg yolk antibody as well as a preparation method and application thereof. The invention relates to an anti-aeromonas cainea egg yolk antibody, which is prepared by immunizing laying hens by using an inactivated vaccine prepared from aeromonas cainea RDBAE-24091, collecting hyper-immune eggs, and separating and purifying the hyper-immune eggs to obtain the anti-aeromonas cainea egg yolk antibody. The method comprises the following steps: carrying out inactivation treatment on aeromonas caviridis RDBAE-24091 to prepare an inactivated vaccine, inoculating the vaccine into a laying hen body, inducing the laying hen to generate a specific immune reaction, enabling an immune system of the laying hen to generate an antibody aiming at the aeromonas caviridis to form a hyper-immune egg, carrying out separation and purification on the hyper-immune egg, removing impurities in egg liquid, and carrying out purification on the hyper-immune egg so as to obtain the laying hen feed. A high-purity and high-activity target product is obtained, and an egg yolk antibody product which aims at the aeromonas cainea and has both green safety and targeted antibacterial efficiency is provided.

The invention discloses a preparation method and application of a traditional Chinese medicine spray for treating asthma, and belongs to the technical field of traditional Chinese medicine preparations. The stability problem of the traditional Chinese medicine spray is systematically solved through the synergistic effect of alcohol precipitation impurity removal, volatile oil clathration, pH regulation and control and the ternary stabilizer. Pharmacodynamic studies show that the spray can remarkably reduce the inflammatory factor level of asthma model mice and prolong the incubation period of asthma inducing of guinea pigs, has the effect better than that of oral granules, takes effect quickly and is suitable for quick relief in the acute attack period of asthma.

The invention discloses a long-acting anti-rabies virus monoclonal antibody as well as a product and application thereof, and relates to the technical field of antibodies. The invention provides an anti-rabies virus monoclonal antibody. The heavy chain gene sequence of the anti-rabies virus monoclonal antibody is as shown in SEQ ID NO: 1. A recombinant human anti-rabies virus monoclonal antibody R7 antibody is subjected to long-acting transformation through a YTE site-directed mutagenesis technology (Fc segment M252Y / S254T / T256E mutation), the half-life period of the R7 antibody in a guinea pig body is remarkably prolonged through YTE mutation, the in-vivo residence time is prolonged, the effective blood concentration is still maintained 56 days after administration, the long-acting effect is definite, and the R7 antibody has the advantages of being long-acting, efficient and safe and has a good application prospect. The clinical administration frequency can be reduced, and the treatment cost is reduced.

1. Name of the product in this design: Gua Sha board (wings). 2. Purpose of this design: A tool for gua sha (scraping therapy). 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention discloses a natural composition for repelling termites and pests, and belongs to the field of pest control. According to the invention, 95-97% pretreated dry cinnamon particles (particle size of 0.2-0.4 cm and water content of 8-10%) are used as a carrier, six plant source active components such as cinnamyl aldehyde and cinnamyl alcohol are compounded, and the synergistic ratio of cinnamyl aldehyde to cinnamyl alcohol is controlled to be 3: 1-5: 1. The preparation method comprises the following steps: preparing an active ingredient solution, and adsorbing and molding the carrier to prepare a granular repellent bag or a spray type preparation. The termite repellent is suitable for closed home environments, wood protection and garden plant scenes and can repel termites, cockroaches and mites in a broad spectrum, the repelling rate within 24 hours is larger than or equal to 95%, and the lasting period is 20-30 days. A guinea pig skin stimulation test proves that the product reaches a non-stimulation level, has no phytotoxicity to plants, has an industrial production batch difference rate of less than 3%, and solves the problems of narrow repellent spectrum, short persistent effect and difficult mass production of the existing product.

The invention provides a construction method of a solar dermatitis guinea pig model and application of the model, and belongs to the technical field of animal models. The invention provides a construction method of a solar dermatitis guinea pig model, which utilizes 2-10J / cm < 2 > UVB dose irradiation to evaluate systems from multiple dimensions such as dynamic skin scoring, skin thickness monitoring, histopathology and the like, and determines that the method can utilize guinea pig back skin to establish an animal model with stable solar dermatitis phenotype. A reliable and normative standardized experimental platform is provided for in-depth study of pathogenesis of solar dermatitis, development of novel protection strategies and preclinical pharmacodynamic evaluation of therapeutic drugs.

This invention relates to the field of biotechnology and discloses a method for screening drugs capable of preventing and / or treating posterior segment diseases of the eye. The method includes: first, decompressing the anterior chamber of an experimental animal; then, pre-establishing a "perforation-closure" channel in the sclera of the experimental animal; next, injecting the drug to be screened into the suprachoroidal space of the experimental animal through the "perforation-closure" channel in the sclera; and finally, evaluating the effectiveness of the drug in preventing and / or treating posterior segment diseases based on changes in the eye condition of the experimental animal. This invention reduces the incidence of ocular surface inflammation and improves safety by lowering intraocular pressure and creating a pre-closed scleral incision to prevent backflow of the injected AAV vector; it also provides a broader expression range and longer duration of action. Studies have shown that injecting shRNA targeting the TGF-β2 gene using the method of this invention inhibits myopia progression in a guinea pig model. The method provided by this invention is beneficial for screening gene therapy drugs related to posterior segment diseases.

The invention relates to the technical field of cell culture, and discloses a tracheal fibroblast and application thereof, the tracheal fibroblast is a guinea pig tracheal fibroblast immortalized cell, and the preservation number of the tracheal fibroblast immortalized cell is CGMCC No.46764. In the invention, the cell has good susceptibility to respiratory pathogens, and has excellent transfection efficiency. The cells can be used for separating or culturing respiratory pathogens, preparing respiratory pathogen vaccines, screening and / or preparing medicines for preventing and / or treating diseases caused by the respiratory pathogens, serving as host cells for researching the respiratory pathogens, detecting the respiratory pathogens in a non-diagnostic manner and constructing respiratory pathogen infection in-vitro models. The method has high scientific research value and practical application potential.

The invention relates to the technical field of cell culture, and discloses a tracheal epithelial cell and application thereof, the cell is a guinea pig tracheal epithelial immortalized cell, and the preservation number is CGMCC No. 46763. In the invention, the cell has good susceptibility to respiratory pathogens, and has excellent transfection efficiency. The cells can be used for separating or culturing respiratory pathogens, preparing respiratory pathogen vaccines, screening and / or preparing medicines for preventing and / or treating diseases caused by the respiratory pathogens, serving as host cells for researching the respiratory pathogens, detecting the respiratory pathogens for a non-diagnostic purpose and constructing organoids. The method has high scientific research value and practical application potential.

The invention discloses a histamine modified collagen matrix material as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The histamine modified collagen matrix material comprises rat tail type 1 collagen, calcium chloride, histamine hydrochloride, recombinant guinea pig liver TGM2, Tris alkali, a PBS buffer solution and dd water. The final concentration of each component is as follows: the rat tail type 1 collagen solution is 1-3 mg / mL; 4-6 mM of a calcium chloride solution; 10-30 [mu] g / mL of a histamine hydrochloride solution; 0.1-0.3 U / mL of a recombinant guinea pig liver TGM2 solution; and neutralizing with Tris alkali until the pH value is 7.2-7.4. Based on two different physiological effects of mediating collagen crosslinking and mediating histamine modification of TGM2, the histamine modified collagen matrix is constructed, and compared with a common collagen matrix, the histamine modified collagen matrix has superior mechanical properties and can be used as a medical material for drug delivery, coating and dressing.

Animal bedding, such as for a guinea pig, is disclosed. The animal bedding comprises a main section and a pocket. The main section includes a top layer, a middle layer, and a bottom layer. The top layer is hydrophobic, the middle layer is hydrophilic and at least a part of the bottom layer is hydrophobic. The top layer, the middle layer and the bottom layer are attached to one another, such as via stitching along a perimeter of the main section. Separately, the top layer and the middle layer are attached to one another, such as in an interior of the main section, to form quilting in the main section. The pocket, which is attached to the main section, allows the guinea pig to burrow therein.