97 results about "Guinea pig" patented technology

The invention relates to the field of aquaculture, and particularly discloses a high-splitting-property aeromonas caviridis bacteriophage and application of the high-splitting-property aeromonas caviridis bacteriophage. The invention relates to a high-cracking aeromonas caviridis phage, which has a preservation name of RKP-AE24001, is preserved in No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing on June 14, 2024, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.45944. According to the invention, a strain of aeromonas cainea is separated, the strain of aeromonas cainea bacteriophage is separated by taking the strain as a host, and the aeromonas cainea bacteriophage has a strong splitting effect on the aeromonas cainea separated in a culture environment.

The invention belongs to the field of biological medicine, and particularly relates to an information rhzomorph array for resisting small cell lung cancer and application of the information rhzomorph array. The invention provides polypeptides with amino acid sequences as shown in SEQ ID NO: 1-30. The invention also provides a medicine for resisting small cell lung cancer. The medicine comprises fusion protein obtained by respectively connecting the channel structural domain of the coliform and the polypeptide with the amino acid sequence shown as SEQ ID NO: 1-30. Pharmacodynamic experiments prove that in each growth cycle, a mouse tumor-bearing model always cannot get rid of the recognition and killing of an information rhzomorph array composed of thirty fusion proteins on tumors. A guinea pig model experiment proves that no toxic or side effect is generated in an animal model when the information rhzomorph array is continuously used for 30 days. The information rhzomorph array can be used as an effective small cell lung cancer treatment medicine.

The invention provides a medicinal and edible composition for treating vitiligo as well as a preparation and application thereof, and the medicinal and edible composition comprises the following components in parts by weight: 1-8 parts of peach kernel, 1-8 parts of galangal, 1-8 parts of angelica dahurica and 1-8 parts of black sesame. According to the invention, the peach kernel, the galangal, the radix angelicae and the black sesame which are homologous in medicine and food are combined, so that the effect of treating leucoderma is exerted. Animal experiments show that the effective rate of the peach kernel, galangal, angelica dahurica and black sesame medicine composition for treating vitiligo can reach 70%, the content of tyrosinase in serum of vitiligo model guinea pigs can be increased, synthesis of melanin is accelerated, meanwhile, the content of alpha-melanin cell stimulant in serum of the vitiligo model guinea pigs can be increased, and the curative effect is good. The generation of skin melanin is promoted, and melanin cells are protected.

The invention provides application of a throat-clearing dropping pill in preparation of a medicine for treating cough caused by ACEI. According to the present invention, the throat heat clearing dropping pill can alleviate ACEI-induced cough and accompanied pharyngeal discomfort symptoms by inhibiting bradykinin accumulation, inhibiting arachidonic acid metabolism, reducing cough high sensitivity, reducing airway hyperresponsiveness and other potential action mechanisms, such that the throat heat clearing dropping pill can be used for preparing drugs for preventing and / or treating ACEI-induced cough and accompanied pharyngeal discomfort symptoms. In addition, the invention further successfully establishes a guinea pig cough inducing experimental model of the ACEI compound cough inducing agent for the first time.

The invention relates to the technical field of biology, and discloses separated Aeromonas caviridis and application of the separated Aeromonas caviridis in preparation of nano-selenium, and the preservation number of the Aeromonas caviridis is CCTCC (China Center For Type Culture Collection) NO: M2025128. The invention provides the Aeromonas cainea with both selenium tolerance and selenium reducing capacity for the first time, and the Aeromonas cainea can tolerate a high-concentration selenium salt culture solution and synthesize biological nano-selenium. A nano-selenium nutrient solution can be prepared from the Aeromonas caviridis Se01 provided by the invention, is applied to selenium-enriched agricultural planting, has a remarkable selenium biological enhancement effect on oilseed rape, and has a wide application prospect.

The utility model discloses a device for freezing a guinea pig trachea through liquid nitrogen spray under a bronchoscope. A cold source device is mounted at one end of the first pipe body, and a nozzle is mounted at the other end of the first pipe body and extends into a guinea pig air pipe; one end of the second tube body is provided with a display screen, and the other end of the second tube body is provided with a lens which is in signal connection with the display screen and extends into the trachea of the guinea pig. The spray head extends into the trachea of an experimental animal, liquid nitrogen in the liquid nitrogen high-pressure storage tank is sprayed into the trachea of a guinea pig from the spray head through the first pipe body, a freezing experiment can be carried out on the trachea of the guinea pig, meanwhile, the lens also extends into the trachea of the guinea pig, and the lens transmits the freezing condition in the trachea of the guinea pig to the display screen. The freezing condition and the treatment condition in the trachea can be observed on the display screen in real time, the trachea of a guinea pig, a rat, a mouse and other small animals can be effectively subjected to freezing treatment, and the treatment condition can be observed in real time.

The invention discloses an application of a compound fresh bamboo juice in a guinea pig asthma model, and relates to the field of medicines, a Hartley albino guinea pig is adopted to construct an OVA induced asthma model, and the model is verified to be successfully constructed through evaluation indexes; evaluating the efficacy of the successfully constructed guinea pig asthma model by using different doses of compound fresh bamboo juice, and evaluating the efficacy of the guinea pig asthma model by scoring guinea pig capsaicin stimulation cough inducing times, alveolar lavage fluid leukocyte number, inflammatory factor content, lung tissue hydroxyproline content and behavioral signs. And evaluating the treatment effect of the compound fresh bamboo juice through Western blot detection, lung histopathology and immunofluorescence staining indexes. In a guinea pig asthma model, high-dose and medium-dose groups of the compound fresh bamboo juice can be used for remarkably reducing the number of cough, reducing the number of white blood cells in alveolar lavage fluid and the level of inflammatory factors and inhibiting pulmonary fibrosis, and a good treatment effect is shown. The results provide powerful support for potential application of the compound fresh bamboo juice in asthma treatment.

The invention relates to a polygonatum sibiricum (guinea pig) soup base and a preparation method thereof, and the preparation method comprises the following steps: a, dicing and cleaning: slaughtering a guinea pig, dicing the guinea pig, soaking the guinea pig in normal-temperature salt water for cleaning, and then cleaning the guinea pig with normal-temperature clean water; b, stewing: draining redundant water on the surfaces of the cleaned tunbird blocks, putting the tunbird blocks into a clean and dry pot, pouring warm boiled water into the pot, simultaneously putting table salt, rhizoma polygonati slices and ginger slices, boiling the water in the pot with big fire until the water is boiled, and continuously stewing with slow fire for 3 hours; and c, taking out of the pot, namely adding white sugar into the pot, melting, uniformly stirring, turning off the fire, and stopping stewing, thereby obtaining the rhizoma polygonati and bird soup base. The preparation method of the polygonatum sibiricum soup base is simple and easy to implement, and the polygonatum sibiricum soup base prepared by the method is excellent in taste, can perfectly reflect the flavor of local specialty tunbirds, and well retains the edible nutritional value of the local specialty tunbirds. According to the method, the local specialty tunn birds can obtain a wider market, and greater economic benefits and social benefits are obtained.

The invention discloses an application and a preparation of a miR-125b-5p inhibitor or antagonist for treating and / or preventing myopia. According to the application, a miR-125b-5p inhibitor or antagonist, particularly miR-125b-5p antiagomir with a nucleotide sequence as shown in SEQ ID NO: 8, is used for inhibiting the expression of miR-125b-5p in sclera and increasing the expression of a target gene tp53, so that the transdifferentiation of sclera fibroblasts can be remarkably reduced, sclera remodeling can be reduced, and the diopter reduction and ocular axis extension of guinea pig deprivation myopia can be retarded; the composition can be used for preventing myopia and controlling myopia progress, has the advantages of low administration frequency and high safety, and provides a safer, more effective and more convenient prevention and control means for myopia patients, especially teenagers.

1. The name of this design product: Eyeglass frame (guinea pig). 2. Purpose of this design product: used to store glasses. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The water dolphin toy comprises a dolphin body, a propeller driving assembly, a rotating ball and a rotating device, the dolphin body comprises a dolphin head part and a plurality of tail section parts which are hinged in sequence, water floating structures are arranged in the tail section parts, the propeller driving assembly is arranged on the dolphin head part, and the rotating ball is arranged on the rotating device. The rotating ball is located at the mouth position of the dolphin head, and the rotating device is located in the dolphin head and can drive the rotating ball to rotate. And a balance iron rod is transversely arranged in the guinea pig head part, so that the driving stability can be improved. The toy is reasonable in structural design, the propeller driving assembly is adopted to replace a traditional fishtailing to provide power, the pushing force is large, the whole toy can rapidly run, and the toy fun is increased; the tail sections are movably connected and can dynamically swing along with fluctuation of waves, the simulation effect is good, and interestingness is achieved; meanwhile, the rotating device drives the rotating ball to rotate, and the entertainment of the toy is greatly improved.

This invention relates to a guinea pig model of chronic obstructive pulmonary disease (COPD) with chronic bronchitis, its preparation method, and its application. Specifically, this invention provides a guinea pig model of COPD with chronic bronchitis, established by subjecting guinea pigs to at least two intratracheal infusions of lipopolysaccharide (LPS) combined with repeated exposure to cigarette smoke. This results in the guinea pigs exhibiting pathophysiological characteristics consistent with those of COPD with chronic bronchitis in terms of cough sensitivity, airway inflammation, airway hypersecretion, lung function, and histopathology. This lays a solid foundation for in-depth research and drug screening of COPD with chronic bronchitis.

The invention relates to a polypeptide for treating rhinitis as well as a preparation method and application thereof, and belongs to the technical field of biology. Natural active small molecule polypeptides are extracted from white tea plants, and the polypeptides are safe and non-toxic to cells and have remarkable bacteriostasis and sterilization effects on rhinitis pathogenic bacteria such as staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger; in addition, in a guinea pig in-vivo test, symptoms of allergic rhinitis and inflammatory factors such as IgE, IL6 and IFN-gamma can be remarkably improved, and the treatment effect is superior to or equivalent to that of a positive control group. The white tea polypeptide disclosed by the invention has a remarkable effect of treating rhinitis, provides a novel, safe and efficient treatment choice for rhinitis patients, and has important scientific value and clinical application prospect.

The invention provides a traditional Chinese medicine composition for treating cough variant asthma and application thereof, and belongs to the field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from radix bupleuri, herba ephedrae, herba schizonepetae, semen armeniacae amarae, radix platycodonis, fructus aurantii immaturus, exocarpium citri grandis, rhizoma phragmitis, rhizoma pinelliae, rhizoma belamcandae, the traditional Chinese medicine composition can obviously improve the condition of weight loss of guinea pigs after combined molding of ovalbumin and capsaicin, reduces the cough frequency and the lung focus area, effectively inhibits inflammatory factors, and can reach the treatment level equivalent to that of montelukast sodium.

The utility model relates to the technical field of intravenous injection of guinea pigs, and discloses an auxiliary device for intravenous injection of guinea pigs, which comprises a working table, a placing groove is arranged on the working table, a first rotating shaft is rotatably connected on the working table, one end of the first rotating shaft is fixedly connected with a first handle, and a first worm is fixedly connected on the first rotating shaft. The auxiliary device comprises a first worm and a second worm, the first worm is meshed with a first worm gear, the first worm gear is fixedly connected with a second rotating shaft, and the second rotating shaft is rotatably connected to a workbench. When the auxiliary device for intravenous injection of the guinea pig is used, the combined design of a U-shaped ring and an elastic band can be tightly attached to the body of the guinea pig; movement and struggling of the guinea pig are effectively reduced, a stable working platform is provided for a laboratory technician, the laboratory technician can more accurately position an injection point, errors caused by movement of the guinea pig are reduced, and therefore the accuracy and success rate of injection are remarkably improved.

The invention discloses an antibacterial peptide, a preparation method and application thereof and an antibacterial drug, and relates to the technical field of antibacterial peptides, the amino acid sequence of the antibacterial peptide is NH2-RRWRIVLIRARK-CONH2, and the preparation method is a polypeptide solid-phase synthesis method. The antibacterial peptide is wide in acid-base stability range from pH 2 to pH 9; the temperature stability range is wide and ranges from-20 DEG C to 50 DEG C; 95% ethanol resistance is realized; the shelf life is stable and exceeds 6 months. According to the antibacterial peptide provided by the invention, the trichophyton mentagrophytes are killed by inhibiting spore germination of the trichophyton mentagrophytes and causing hypha breakage, the trichophyton mentagrophytes do not generate drug resistance to the antibacterial peptide, the 1% concentration of the antibacterial peptide has an obvious treatment effect on a guinea pig model infected by the trichophyton mentagrophytes, and the antibacterial peptide has a good application prospect as a novel candidate drug for treating trichophyton mentagrophytes infection.

The utility model relates to the related technical field of animal feeding, in particular to an independently ventilated guinea pig cage for experiments, which comprises a cage body, an isolation plate is arranged on the cage body, a buckle is arranged outside the cage body, a cage cover is arranged on the cage body, a sealing mechanism is arranged in the cage cover, a conveying mechanism is arranged in the isolation plate, and a kettle is arranged outside the isolation plate. According to the independent ventilation cage, through the arrangement of the cage body, the isolation plate, the buckle, the cage cover, the sealing mechanism, the conveying mechanism, the kettle and the fixing plate, when ventilation is conducted on the cage, a ventilation pipe is inserted into a ventilation column firstly and then continues to be inserted, at the moment, a spring on the pressing column is compressed due to insertion of an air inlet pipe, an air inlet is exposed, and then clean air can be pumped into the cage; the filter screen can prevent reverse pollution, the column is pressed to return after the pipe is pulled out, polluted gas in the cage is prevented from escaping, the exhaust method is similar, but the filter screen of the exhaust valve needs to be taken out in advance, otherwise, the gas is blocked and cannot be exhausted, and therefore the sealing performance is guaranteed.

The invention discloses a pharmacodynamic test method for a cough relieving mixture, which comprises the following steps: constructing four core pharmacodynamic test sub-models containing ammonia water to cause young mouse cough, citric acid to cause young guinea pig cough, rabbit in-vitro trachea cilia mucus movement and young mouse trachea phenol red excretion and a body model of a test result analysis sub-model; each pharmacodynamic test sub-model is provided with a plurality of groups of test arrays and test individuals; pathogen data is imported to generate a simulated disease scene, and a corresponding drug sequence is called to intervene and collect physiological index change data; and multi-dimensional pharmacodynamic parameters are evaluated through the analysis sub-model, and a dose-effect relation curve and an evaluation report are generated. According to the invention, a parallel verification system for antitussive and expectorant tests is formed, the antitussive effective dose, the expectorant effective dose and the effective dose range under the dual action of the medicine are determined through multi-model cross validation, the accuracy degree of judging the effectiveness of the medicine is greatly improved, and a reliable method support is provided for evaluating the efficacy of the cough-stopping mixture.

The invention provides a whale-dolphin sound signal analysis method, system and device and a medium, and relates to the technical field of wild animal protection. Comprising the following steps: acquiring an audio digital signal recorded by a target underwater recorder; carrying out filtering processing on the audio digital signal by using a band-pass filter, and carrying out fast Fourier transform after filtering processing to obtain a corresponding time frequency signal; performing pulse signal screening operation on the time-frequency signal according to a preset constraint condition to obtain a first pulse signal sequence meeting the characteristics of the target whale-guinea pig pulse signal; the preset constraint condition is that the ratio of the energy of the time frequency signal to the energy of the audio digital signal under the current time resolution is greater than or equal to a preset proportion threshold value, and the time interval of the two pulse signals is at least a preset interval number of time resolutions; and screening the first pulse signal sequence according to the characteristics of the pulse signal of the target whale guinea pig to obtain a second pulse information sequence. The scheme can be used for quickly and accurately identifying and analyzing the sound signals of the whale and guinea pigs.

The invention provides a construction method of an out-of-focus type myopic animal model and a myopic animal model diagnosis device and equipment, and belongs to the technical field of biomedicine. According to the method, a myopic guinea pig model is manufactured by adopting a monocular hyperopia optical defocus method, a Photorefrator small animal infrared optometry unit is applied to the measurement of the eye refraction degree of the out-of-focus myopic model guinea pig, and the development degree of the out-of-focus myopic model guinea pig is quantitatively and objectively judged according to the absolute value of a relative myopic refraction value. An ultrasonic measuring instrument is adopted in parallel to measure the eye axis length of a model animal to assist in judging the development of the model, and the out-of-focus myopia animal model is successfully constructed. The animal model can be applied to pharmacodynamic evaluation of non-clinical ophthalmic drugs, provides more reliable drug effectiveness data support, and lays a foundation for new drug research application and clinical application of new drugs.

The invention provides guinea pig pluripotent stem cells capable of being stably passaged in vitro and a method for constructing pluripotent stem cells capable of being stably passaged from guinea pig embryos.

The application provides a preparation method of Artemisia sphaerocephala pollen allergen and an Artemisia sphaerocephala pollen allergen injection, and belongs to the technical field of pollen allergen vaccine preparation. After the Artemisia sphaerocephala pollen is defatted and dried, the Artemisia sphaerocephala pollen is extracted by using an extraction solution, the supernatant is filled into a dialysis bag to be dialyzed, concentrated, enzymolyzed and desalted, and then chromatography is carried out, and polypeptides with molecular weights of 23-46KD and 67-82KD are collected to be the Artemisia sphaerocephala pollen allergen. The Artemisia sphaerocephala pollen allergen injection comprises the Artemisia sphaerocephala pollen allergen, sodium chloride, PBS buffer, phenol and thiourea. The Artemisia sphaerocephala pollen allergen injection of the application fills the blank of Artemisia sphaerocephala pollen allergen injection, can effectively reduce the allergic degree of guinea pigs to Artemisia sphaerocephala pollen, and can be stably stored at 2-8 DEG C.

The invention pertains to pet care products, specifically: litter box fillers for animals (cats, hamsters, rabbits, guinea pigs, terrariums, and others) living in human dwellings, and can also be used as bedding / flooring in chicken coops, horse stables, and livestock farms. The animal litter box filler is manufactured in granular form from recycled waste paper in the form of newspapers, books, corrugated cardboard, and natural additives. The raw material undergoes a series of steps: shredding, fluffing, and moistening to 15-25%, resulting in finished granules with a diameter of 4-12 mm and a length of 6-18 mm. The filler manufacturing process includes preliminary raw material sorting, subsequent shredding, fluffing, moistening, and then granulation and cooling of the granules. In the granulation machine, the raw material is pressed through round openings using a die die.The technical result consists of expanding the range of fillers and increasing the filler absorbency, as well as increasing the service life when stored in a dry place and convenient disposal into the sewer system or food waste for further processing.

The invention belongs to the technical field of animal model construction, and particularly relates to a spinal cord hypertension syndrome disease model modeling method and application. A guinea pig or a macaque is used as an experimental animal, and a composite biostimulation material and an accurate pressure regulation and control modeling system are combined to realize modeling. The composite biostimulation material is composed of a core pressure slow-release layer, a middle biocompatible coating and a surface targeting binding layer, has excellent biocompatibility, degradability and targeting property, and can reduce non-specific inflammatory response; the precise pressure regulation and control modeling system comprises a pressure sensing module, a micro-injection module, a real-time monitoring module and a main control module, and can dynamically regulate and control the pressure in the spinal cord in a closed-loop mode. The modeling method is easy and convenient to operate and good in repeatability, the model building success rate exceeds 95%, the pathological features are highly similar to clinical features and can last for 4 weeks, a reliable experimental model is provided for mechanism research, drug screening and therapeutic schedule evaluation of the spinal cord high pressure syndrome, and the modeling method is suitable for large-scale popularization.

The invention discloses an application of apigenin-7-O-beta-D-glucuronide in preparation of a medicine for treating fundus diseases, and establishes a model of ischemic fundus diseases of guinea pigs and a model of eyeball conjunctival microcirculation disturbance of rabbits. The prevention and treatment effects of the apigenin-7-O-beta-D-glucuronide on fundus diseases and fundus lesions are researched, and an experimental basis is provided for clinical treatment of the fundus diseases by the apigenin-7-O-beta-D-glucuronide.

The utility model belongs to the field of experimental animal equipment, and particularly relates to a guinea pig fixing frame which comprises a bottom plate, an operation table is arranged above the bottom plate, an adjusting frame is arranged between the bottom plate and the operation table, and the adjusting frame can change the inclination angle of the operation table and the height of the operation table. A plurality of adjusting holes are formed in the operating table and used for fixing four limbs of a guinea pig, and an elastic belt is further arranged on the operating table; the adjusting frame comprises two first supports and two second supports, the two first supports are oppositely arranged, the two second supports are oppositely arranged, the tops of the first supports are fixed to the bottom of the operation table, the bottoms of the first supports are fixed to the bottom plate, a hinge support is arranged between the two first supports, and the hinge support comprises a connecting rod and a sliding rod. One end of the connecting rod is connected to the first support, the other end of the connecting rod is connected with the end of the sliding rod, and the connecting rod is connected with the first support through a screw. The guinea pig fixing device can safely and stably fix a guinea pig, and experiment operators are prevented from being scratched by the guinea pig.

This invention relates to the field of microbial technology, specifically disclosing a thermoresistant Aeromonas verrucosa phage, its screening method, and its applications. The phage is deposited under CGMCC No. 47009. This phage belongs to the Myocaudidae family and exhibits significant heat resistance, maintaining a titer of 10 even after treatment at 70°C for 60 minutes. ^ 8 This bacteriophage exhibits a PFU / mL concentration above [amount missing] and can withstand the high temperatures of feed pelleting. It displays broad-spectrum lytic activity against *Aeromonas vesiculosus* and can cross-lyze *Aeromonas guinea pig* and *Aeromonas hydrophila*. This invention also provides a rapid screening method for this heat-resistant bacteriophage. Through a cycle of instantaneous heat treatment at 70°C followed by rapid cooling in an ice bath, genetically stable bacteriophages can be obtained within 5 days, solving the problems of long cycles and poor stability associated with traditional acclimatization methods. The bacteriophage and composition of this invention can be used to prepare veterinary drugs and disinfectants for the prevention and treatment of aquatic aeromonas diseases, showing broad application prospects.