39results about How to "Simple detectability" patented technology

The invention provides a storage battery residual capacity and health state rapid detection method and device. The method comprises the following steps of: enabling a storage battery to carry out instant larger current discharging through a controllable discharge circuit, monitoring the discharging current in the discharging circuit in the process by a current sampling circuit, synchronously monitoring the end voltage of the storage battery through a voltage sampling circuit, and utilizing a direct current discharge method to measure the internal resistance of the storage battery, wherein the discharge circuit and the voltage sampling circuit are connected in parallel with two wiring columns of the storage battery, so that the internal resistance of the storage battery is measured according to a Kelvin four-wire system measuring method, and the maximum discharge current, the load voltage and the internal resistance data of the storage battery are obtained; and according to an associated relation model, calculating SOC and SOH of the storage battery. By adopting the method provided by the invention, the residual capacity and the health state of the storage battery can be accurately detected on line.

The invention relates to a staining agent combination for tumor tissue cells, in particular to a staining agent combination for the tumor tissue cells. The combination comprises 0.01%-5% of a color developing agent, less than or equal to 6.5% of folic acid, 0.1%-10% of a leuco agent, less than or equal to 10% of a pH modifier and 3%-95% of water, wherein the color developing agent adopts methylene blue and/or dichlorophenolindophenol; the leuco agent adopts a reducing reagent; a targeted drug preparation adopts the folic acid; and the solution pH modifier and the water are adopted. The coloring agent for staining tumor cell can also be used for detecting the tumor cells. The invention also relates to a method of the staining agent combination used for detecting the tumor cells, a kit for detecting the tumor cells and a detection carrier.

The related testing mark for projection objective image difference of photo-etching machine comprises a square inner mark and four external group marks around the inner one. Wherein, every external group of marks includes at least two lines with different width to periodical close arrange and form the marks; and the periods of all four groups are same. This invention simplifies the process and algorithm, and can achieve high testing precision.

The invention discloses an ELISA agent box to test Asial foot-and-mouth disease virus and making method of the agent box, which comprises the following parts: solid support to connect the detected sample, mono-clone antibody to clad Asial foot-and-mouth disease virus on the solid support connected with the detected sample, multi-clone antibody of guinea pig as dianti-Asial foot-and-mouth disease virus, rabbit anti-guinea pig lgG marked by enzyme, standard serum, colorless substrate, terminal liquid.

The invention discloses an image registration method based on contour detection and feature matching. The image registration method comprises the following steps that firstly an image to be registered and a standard image are expanded through black/white filling; then threshold segmentation is performed on the image to be registered; then contour detection is performed on a threshold image, and the contour of the image target area is selected as a preliminary contour image; then contour detection is performed on the preliminary contour image from the top to the bottom, and a minimum enclosing rectangle is established; then the absolute value theta of the rotation inclined angle between the top rectangle and the horizontal axis of the anticlockwise direction and the coordinates pt[0], pt[1], pt[2] and pt[3] of the four vertexes are calculated; then the rotation angle is judged to be positive/negative according to pt[0] and pt[1] and then a preliminary registration image is acquired by using affine transformation according to -theta or 90 degrees-theta; and finally matching based on the feature points is performed on the standard image and the preliminary registration image so as to acquire the final registration image. Rapid and high-precision registration of the image of the rotation angle of 0-90 degrees and any translation deviator can be realized so as to meet the requirements of various markets requiring image registration.

The invention provides a benzimidazole quinoline derivative, a preparation method and application in copper ion detection, and particularly relates to a 2-(4-N,N-bis-substituted styryl)-8-1H-benzimidazole) quinoline derivative. Particularly, 2-aminobenzoic acid is used for preparing 2-methylquinoline-8-carboxylic acid, then the 2-methylquinoline-8-carboxylic acid and o-phenylenediamine are subjected to condensation to obtain 2-methyl-8-(2-benzimidazolyl) quinoline, the 2-methyl-8-(2-benzimidazolyl) quinoline and N,N-bis-substituted benzaldehyde are subjected to condensation to obtain the benzimidazole quinoline derivative. A probe has the good complexation with copper ions, when the copper ions exist, derivation of the solution is observed with naked eyes, and the solution is changed from yellow to purple, absorption at the 418 nm position on an ultraviolet visible absorption spectrum is weakened, and a novel absorption peak appears at the 550 nm and gradually increases when the concentration of the copper ions is increased; the benzimidazole quinoline derivative can be used for real-time and rapid measurement of complex samples and can also be used for semiquantitative detection of micro/trace Cu2+ of environment samples from different sources.

The invention discloses a screening method of nucleic acid aptamers for specifically recognizing streptomycin. The screening method comprises the following steps: (A) coupling epoxy group gel with streptomycin; (B) preparing a negative electrode pole and a positive electrode pole; (C) constructing an ssDNA random library, and screening and determining aptamers with relatively high affinity to streptomycin; and (D) authenticating the aptamer with the highest affinity for specifically recognizing streptomycin by virtue of a fluorescence method. According to the screening method, the screened nucleic acid aptamer is utilized for qualitatively and quantitatively determining streptomycin in food. The screening method is high in condition preciseness, other interference factors are easily eliminated, the screened nucleic acid aptamer has very high affinity and high specific recognizability, and furthermore, a false positive phenomenon can be reduced; the screening method is a novel method for simply, rapidly, scientifically and accurately screening the nucleic acid aptamers; by combining the screened nucleic acid aptamer with a colloidal gold colorimetric method, streptomycin can be simply, rapidly and accurately detected; and the screening method can be widely applied to various fields of foods, sanitation, import and export inspection and the like.

The invention discloses an astaxanthin synthetic gene recombinant expression plasmid pET-Ast with a base sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the recombinant expression plasmid pET-Ast, an application of the recombinant plasmid pET-Ast to the detection of astaxanthin synthetase activity of a target receptor, and an application method. After the astaxanthin synthetic gene recombinant expression plasmid pET-Ast provided by the invention is introduced to an Escherichia coli BL21 strain, the accumulation of astaxanthin in a cell body of Escherichia coli is successfully realized, so that an astaxanthin production strain can be accurately and rapidly established. The preparation method of the astaxanthin synthetic gene recombinant expression plasmid pET-Ast is simple and convenient, easy to operate and low in cost. By using the method disclosed by the invention, a gene sequence to be detected in the target receptor can be replaced with a related gene sequence in the recombinant expression plasmid pET-Ast through digestion and connection, and then, the function of a gene to be detected in the target receptor can be simply and effectively detected and verified through detecting whether the astaxanthin is accumulated in a host cell body.

The invention discloses a core primer composition for Brassica SSR (simple sequence repeats) and belongs to the technical field of molecular inheritance. The core primer composition comprises 190 core SSR primer pairs for 19 chromosomes in Brassica A and C genomes; and 10 SSR primer pairs are uniformly distributed on each chromosome, which have high polymorphism levels, substantially consistent annealing temperature and clear amplification band patterns. The core SSR primer composition is applied to increasing the molecular marking efficiency by 3 to 5 times without increasing the working costs, and is particularly suitable for the identification, the genetic diversity analysis, the nullisome and abnormal chromosome individual identification and the seed purity determination of the Brassica germplasm resources. The core SSR primer composition has the advantages of simpleness in operation, high stability and good reproducibility.

The invention provides a molybdenum sulfide/cesium lead halide perovskite quantum dot photoelectric detector and a preparation method and relates to the technical field of a photoelectric detector. The photoelectric detector includes a substrate layer, and two surface electrodes separated from each other are arranged on the substrate layer; a two-dimensional molybdenum sulfide film layer is arranged on the substrate layer between the two surface electrodes, and a cesium lead halide quantum dot layer is further arranged on the two-dimensional molybdenum sulfide film layer. The invention furtherprovides a method of preparing the above photoelectric detector, the surface of the quantum dot film is modified by a ligand, and thereby preparation of the photoelectric detector is completed. The required electrodes are prepared on the substrate layer, a molybdenum sulfide nano film material is coated on a substrate, and the nano film material is covered with the cesium lead halide perovskite quantum dot film. The photodetector has the fast response speed, the response time is less than 100mus, high sensitivity and wide application range are achieved, the preparation method is simple, fastand efficient, simple and easy to operate, has low cost, is green and environmentally friendly and is suitable for large-scale production.

The invention discloses a method for extracting and detecting polyphenol in bamboo shoots. The method comprises the following steps: (1) preparing materials, namely, selecting fresh bamboo shoots, freezing in liquid nitrogen, knocking to shell, and preserving the bamboo shoot meat at minus 70 DEG C for later use; (2) selecting acetone as an extraction solvent, and extracting polyphenol in the bamboo shoots through an ultrasonic low-temperature auxiliary solvent; (3) detecting by using a Folin-ciocalteu method, wherein the detection system comprises 0.25ml of polyphenol extracting solution, 1.75ml of water, 1ml of Folin-ciocalteu color developing agent which is diluted for 6 times and 1ml of 5-15% Na2CO3; the detection condition is that the reaction temperature is 25-35 DEG C in water bath, the reaction time is 0.5-3.5 hours, and the detection wavelength is 755-770nm. Due to adoption of the method, a system for detecting the polyphenol in fresh bamboo shoots and a system for extracting the polyphenol in the bamboo shoots are optimized and established, the detection is rapid and convenient, the extraction efficiency is high, and theoretical foundation can be provided for bamboo shoot development and utilization.

The invention discloses a system and a method for ultrasonically testing multi-layer interface welding quality of a bend composite part. The ultrasonic testing system comprises a centering tool, a flexible pipe, a probe, a driving mechanism and a flaw detector, wherein the centering tool is used for being inserted into an elbow pipe and is in a shape of a hollow pipe; a window communicated with inner and outer side faces of the centering tool is formed in the side wall of the centering tool; annular bulges are formed in the upper and lower sides of the window; the annular bulges are positioned on the outer side face of the centering tool and is in clearance fit with the inner wall of the elbow pipe; the centering tool, the annular bulges and the flexible pipe are made of plastics; an elbow pipe composite part comprises a first metal pipe formed by a first metal, a second metal pipe formed by a second metal, and a block body which is formed by a third metal and is provided with a through hole; the second metal pipe is inserted into the through hole and is welded with the block body; the first metal pipe is an elbow pipe, is inserted into the second metal pipe and is welded with the second metal pipe. According to the technical scheme, accurate positioning and quantitative determination of welding quality defects of two interfaces of the bend composite part are realized.

A constant displacement quality control method of steel rail flame welding heating and post-weld normalizing comprises the following steps: locking an upsetting oil cylinder by controlling a magnet valve and a pilot operated check valve on the stock cylinder oil circuit of the upsetting oil cylinder so that the longitudinal displacement of the steel rail keeps constant; simultaneously, detecting the retaining pressure of the upsetting oil cylinder in real time through a pressure sensor, and then calculating and obtaining the temperature of the steel rail according to the curve of the relationship between the preset welding temperature of steel rail and the longitudinal deformation resistance of steel rail; when the temperature of steel rail reaches the preset welding temperature, controlling a heating device to stop heating, carrying out upsetting pressure retaining shearing, air-cooled cooling and post-weld normalizing. The method is capable of automatically controlling the temperature of gas-pressure welding and pressure retaining and normalizing of steel rail in closed loop, thereby having few control input parameters, simple and convenient operation, accurate control for temperature, strong anti-interference, small error, high precision, good technical uniformity and repeatability, more stable and reliable welding quality, and high one-time welding success ratio.

The invention discloses a supporting leg contracting state detecting method and device for a special vehicle. The method includes the steps of firstly, installing the detecting device; secondly, pushing a detecting rod to slide upwards through a collision block when a perpendicular movement leg contracts; thirdly, driving the detecting rod to move leftwards when a horizontal movement leg contracts; fourthly, triggering a detecting switch to send out a contracting state signal through the detecting rod when the perpendicular movement leg and the horizontal movement leg both reach the contracting positions; fifthly, sending out a disengagement signal through the detecting switch when any one supporting leg leaves the contracting position; sixthly, obtaining a vehicle driving state signal through a controller by means of a parking brake and a gear position, and starting an alarm device to give an alarm when the controller receives a supporting leg disengagement contracting state signal. By means of the detecting device, the contracting states of the perpendicular movement leg and the horizontal movement leg can be detected at the same time, the detecting and control principle is simple, no large electrical system or complicated electrical layout is needed, and cost and damage risk are reduced.

The present invention provides a spindle unit for a machine tool. The spindle unit comprises a spindle housing and a spindle motor which is equipped in the spindle housing. The spindle motor is provided with a rotor which is configured on the spindle and a stator that is fixed relative to the spindle housing relative to a stator bracket. Furthermore the spindle motor is provided with a front spindle bearing unit and a back spindle bearing unit. The front spindle bearing unit and the back spindle bearing unit support the spindle according to a rotatable mode around an axis of the spindle. For improving the spindle unit and facilitating assembling and maintenance of the spindle unit under a precondition that accuracy requirement is kept, the invention suggests that the front spindle bearing unit and the back spindle bearing unit can be connected to one unit with the stator bracket at an assembling state. The unit comprises the spindle motor and the spindle and furthermore can be placed in the spindle housing as a whole member or detached from the spindle housing. Furthermore at the operation state, at least the front spindle bearing unit can be accurately positioned and fixed relative to the spindle housing through a matching surface.

The invention relates to a method for detecting manganese content in graphene oxide and graphene samples. The method is characterized by comprising: drawing a standard curve diagram of a relationship between absorbance and manganese content, measuring absorbance of a sample, and searching the corresponding manganese content from the standard curve diagram according to the absorbance of the sample. The method has the following characteristics that: the method is simple and easy to achieve, and detection precision of the manganese content can meet requirements.

The invention relates to the technical field of spring detecting devices, and discloses an automatic spring detecting device comprising a detecting platform, wherein the upper end of the detecting platform is fixedly connected with two vertically arranged support plates that are respectively located at both sides of the upper end of the detecting platform, two vertical moving plates are disposed between the two support plates, the opposite ends of the two moving plates are respectively provided with T-shaped sliding grooves, a set of T-shaped sliders are slidably connected inside the two T-shaped sliding grooves, both ends of each set of two T-shaped sliders are fixedly connected with L-shaped rods at both ends of the outside of the T-shaped sliding grooves, the vertical ends of the two L-shaped rods are oppositely arranged, and vertically arranged two-way threaded rods are provided inside the T-shaped sliding grooves. The automatic spring detecting device is simple in operation, and can quickly detect the tensile strength and the spring force coefficient of the spring, improve the detection efficiency of the spring, and protect during the detection to prevent the spring from breaking and causing damage to the human body.

The invention provides a rack gear-sector pair with a variable transmission clearance characteristic and an automobile steering gear. According to the rack gear-sector pair, an intermediate tooth meshing clearance of a gear-sector and a rack function part corresponding to the intermediate meshing position of the steering gear is 0, with a gear face meshing line with the intermediate tooth meshingclearance of the rack function part being 0 as a datum, tooth shapes of rack intermediate teeth are modified symmetrically to two sides based on original shapes, and a transmission clearance is gradually increased to a fixed value correspondingly. The automobile steering gear adopts the rack gear-sector pair, in order to achieve the variable transmission clearance characteristic, the racks of therack gear-sector pair (the rack functional part, the gear-sector) of the automobile steering gear are modified and the tooth shapes of the rack intermediate teeth are modified symmetrically to two sides based on the original shapes, the transmission clearance is gradually increased to the fixed value correspondingly, and a rack tooth shape correction method is adopted to obtain an idea variable transmission clearance characteristic curve.

The invention discloses a method for rapidly determining the content of polyunsaturated fatty acids in oil-containing microorganism cells. The method is used for mainly detecting the relative content of polyunsaturated fatty acids in the cells in microorganisms including microalgae, bacteria, fungi, yeast and the like; after a cell culture solution is centrifuged, the bacteria are re-suspended in a buffering solution, and a nuclear magnetic resonance 1H spectrum or 13C spectrum is determined; and the contents of polyunsaturated fatty acids of the different samples can be compared according to the strength ratio of a characteristic peak on a nuclear magnetic resonance spectrogram. Compared with a traditional fatty acid content determination method, operations including sample drying, cell crushing, grease extraction, transesterification and the like are not needed and an organic solvent is not needed, so that manpower and material resources are saved and the determination efficiency is greatly improved; and the method is suitable for high-throughput rapid detection of a lot of samples, such as microorganism screening, optimization of culture conditions and monitoring of an industrial production process.

The invention discloses a data consistency detection and repair method and device and a medium, and relates to the technical field of storage. According to the method, consistency detection is carried out on the files, and when it is detected that the files are inconsistent, the files are repaired. Obtaining a data object of the to-be-repaired file in the repairing process and determining the to-be-repaired data object; and repairing the to-be-repaired data object according to the copy data corresponding to the data object. Therefore, according to the method, verification information does not need to be attached to the whole IO path in the process of realizing the data consistency, so that the data consistency is relatively easily realized; secondly, whether the files are consistent or not is judged firstly, then the to-be-repaired files are divided into the multiple data objects, the files with consistent data do not need to be divided into the multiple data objects, and therefore the time for determining the to-be-repaired data objects is shortened, the data consistency detection and repair process is relatively simple, and the data consistency detection efficiency is improved. And the data consistency detection and repair efficiency is improved.

The invention discloses an exploitation method of an orchid microsatellite marker site, and a detection method of the length of a microsatellite marker in the microsatellite marker site. The exploitation method comprises the following steps: obtaining a mixed sample; extracting the genome of the mixed sample; fragmenting the genome to obtain genome fragments; respectively hybridizing a probe group with the genome fragments; purifying the successfully hybridized genome fragments in a hybridization solution; mixing the purified hybridized genome fragments, and detecting the purified genome fragments through using high flux sequencing; obtaining effective high flux sequencing fragments; and classifying the effective high flux sequencing fragments. The detection method comprises the following steps: selecting a microsatellite marker site to be detected; and amplifying the microsatellite marker in the microsatellite marker site to be detected by using a multiple amplification primer to obtain the length of the microsatellite marker in the microsatellite marker site. The methods are simple, fast, comprehensive and accurate.

The invention discloses a method for identifying L-phenylalanine. The method is characterized by using a fluorescent probe as an identification reagent; and the fluorescent probe is prepared by a seven-membered cucurbituril or an eight-membered cucurbituril and fibrauretin. The method disclosed by the invention has the advantages of simple identification method, low identification cost and high selection unicity.

The invention provides a high-voltage interlocking detection circuit, a high-voltage interlocking detection system and an automobile. The high-voltage interlocking detection circuit comprises at leasttwo test resistors and at least two high-voltage connector interlocking devices, wherein the first ends of respective test resistors are connected together to form a detection point; the second end of each test resistor is grounded through the corresponding high-voltage connector interlocking device; the detection point is connected to a reference voltage; each test resistor is connected to a corresponding high-voltage connector interlocking device; and each test resistor has a different resistance value. The high-voltage interlocking detection circuit can ensure the operation safety of the automobile, and is simple in hardware layout and detection strategy, and is reduced in design cost.

The invention relates to a method for detecting gypenoside A in a gelan Xinning soft capsule by adopting an HPLC-UV method. The gelan Xinning soft capsule is subjected to pretreatment and is measuredby adopting a high performance liquid chromatograph (HPLC-UV), and the measurement result is that the gypenoside contained in each gelan Xinning soft capsule is calculated by gypenoside A (C46H74O17)and is not less than 0.5mg. The detection method is good in specificity, good in instrument precision, good in linearity, precision, accuracy and durability, simple, convenient and accurate in processing and detection processes, and suitable for detection application in production of the gelan Xinning soft capsules.

The invention discloses laboratory anaerobic fermentation equipment and an anaerobic fermentation method.The laboratory anaerobic fermentation equipment comprises a bottle body, a rubber plug is arranged at an opening of the bottle body, the rubber plug can seal the bottle body so as to guarantee the sealed oxygen-free environment in the bottle body, and a sampling pipe for sampling detection is arranged on the rubber plug; the rubber plug is provided with an alkali adding pipe and an air outlet pipe ; the alkali adding pipe is used for adjusting the pH value and adding alkali liquor, the air outlet pipe is used for adjusting the air pressure in the bottle to ensure that the bottle body is not damaged, the anaerobic fermentation process can be completed, the method is easy to operate, the overall sealing effect is good, correct experimental data can be obtained, and the problems of high cost and much waste caused by experiments in the prior art are solved. Different from the problem that the existing simple fermentation device cannot be randomly sampled, the laboratory anaerobic fermentation equipment is extremely high in flexibility and is very suitable for reducing the experiment cost in a laboratory.

The invention discloses a press holding-up hammer crack detection method which comprises the following steps: step 1, mounting an acoustic emission detection module at the front end of a press holding-up hammer, and collecting acoustic wave signals on a press working site in real time through the acoustic emission detection module; step 2, processing the sound wave signal acquired in the step 1 into a signal with a proper signal-to-noise ratio and amplitude voltage by adopting a signal adjustment module to obtain an analog sound wave signal; 3, the analog sound wave signals are converted into digital sound wave signals through data acquisition equipment, and the digital sound wave signals are stored in storage equipment of a press control cabinet; 4, compiling the sound wave signal identification algorithm into an application program by adopting a hybrid programming mode, and integrating the application program into a press control system; and 5, an application program is called through a man-machine interaction screen of a press control cabinet, the digital sound wave signals are preprocessed, anvil crack sound wave signals are recognized, if the anvil crack sound wave signals are recognized, an alarm is output to a press control system, and the press is controlled to stop heating and relieve pressure.

The invention relates to a particle size testing method of nano zirconium dioxide, which sequentially comprises the following steps: (1) adding nano zirconium dioxide powder into a dilute acetic acid solution, and uniformly stirring to obtain a first mixed solution; (2) putting the first mixed solution into an ultrasonic disperser, and performing ultrasonic dispersion until the nano zirconium dioxide powder in the first mixed solution is completely dispersed; (3) adding a proper amount of sodium hexametaphosphate solution into the first mixed solution, and uniformly stirring to obtain a second mixed solution; (4) carrying out ultrasonic dispersion on the second mixed solution for 5-10 minutes by utilizing an ultrasonic disperser; and (5) opening the laser particle analyzer, sampling from the second mixed solution, and adding into the laser particle analyzer for testing. The method is simple in detection instrument and treatment equipment, low in investment, simple to operate, low in price of required reagents, low in usage amount and free of environmental pollution; and the detection result is high in accuracy and good in repeatability.

The invention relates to an electronically commutated electric motor (10), in particular an electronically commutated electric motor. In order to detect the angle or rotational speed of rotation accomplished by a rotor shaft (16) of an electric motor (10), the rotor shaft (16) is equipped with a signal transmitter (24) of a sensor device. The signal transmitter has a magnet element (26) which is fixed to the rotor shaft (16) by means of a retaining element (28). The invention relates to a clamping body (46) which is arranged on the rotor shaft (16) in a force-transmitting manner and onto which the holding element (28) is pressed.

The invention discloses a method for removing high-concentration iron-sulfur and heavy metal pollutants in acid mine wastewater, which comprises the following steps of: firstly, introducing the acid mine wastewater into an adjusting tank by using a water pump, and properly adding water into the acid mine wastewater in the adjusting tank according to the initial pH value of the acid mine wastewater to dilute the acid mine wastewater so that the pH value of the acid mine wastewater is increased to about 4; high-concentration iron ions in the wastewater are self-hydrolyzed to form Schwertmannite, and the Schwertmannite and heavy metal pollutants such as mercury, cadmium and arsenic are co-precipitated and removed; the iron-removed acid mine wastewater is filtered by the vertical underflow retarding belt, and the filtered acid mine wastewater enters the three-stage dynamic ecological filter tank and is subjected to physical adsorption, ion exchange, precipitation and the like; according to the method for removing the high-concentration sulfate ions, the iron ions and the heavy metal pollutants in the acid mine wastewater, the utilization rate of water is increased, the treatment cost is reduced, the water can be repeatedly recycled, and the method is environmentally friendly and free of pollution. And the continuity of the treatment effect is improved.

The invention discloses an image false color processing method. The image pseudo color processing method comprises the steps of obtaining a real-time image in a YCrCb format; Calculating a pseudo color processing factor of each pixel of the real-time image according to the spatial correlation characteristic and the spectral correlation characteristic of the real-time image; Obtaining a distribution map of the pseudo color information on the real-time image according to the pseudo color processing factor of each pixel; And adjusting the chromaticity of the pixel according to the pseudo color processing factor of each pixel to obtain a pseudo color removed image. In addition, the invention also discloses an image false color processing device. According to the image pseudo-color processing method and the image pseudo-color processing device, pseudo-color removal processing is directly carried out on the real-time image with the color space being YCrCb, the pseudo-color detection and pseudo-color processing method is simple, and the calculated amount is small.