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Method for identifying L-phenylalanine

A technology for phenylalanine and identification methods, applied in chemical instruments and methods, instruments, analytical materials, etc., can solve the problem of low specificity, and achieve the effect of simple detection process and equipment, low cost, and simple identification methods

Active Publication Date: 2019-10-25
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are fluorescent probes (patent number: CN201811586962.4) prepared by eight-membered melon ring and patinol, which are used to detect and identify iron ions in drinking water. The patent (patent number: CN201811222729.8) discloses a Fluorescent probe, prepared from eight-membered cucurbit ring and acridine hydrochloride, can recognize L-phenylalanine, but it can also recognize L-arginine, L-histidine and L- Lysine, etc., the specificity is not high

Method used

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  • Method for identifying L-phenylalanine
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  • Method for identifying L-phenylalanine

Examples

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Effect test

Embodiment 1

[0042] Embodiment 1: A method for identifying L-phenylalanine, the steps are as follows:

[0043] 1) Preparation of standard probe solution: Dissolve seven-membered melon rings in water to obtain solution A; dissolve patinol in water to obtain solution B; mix solution A with solution B to control seven-membered melon rings and patinol The molar ratio is 1:1, react at room temperature to obtain a probe solution, in which the molecular formula of the probe is C 42 h 42 N 28 o 14 @C 21 h 22 NO 4 , the probe model diagram is attached image 3 As indicated, the probe solution was diluted with neutral water to a concentration of 2.0×10 -5 mol / L, to obtain the standard probe solution;

[0044] 2) L-phenylalanine recognition: Add the solution to be tested dropwise into the standard probe solution, let it stand for 10s, then measure the fluorescence spectrum at a fixed excitation wavelength of 342nm, and draw the fluorescence intensity at the excited laser wavelength When the ...

Embodiment 2

[0045] Embodiment 2: A method for identifying L-phenylalanine, the steps are as follows:

[0046] 1) Preparation of standard probe solution: Dissolve seven-membered melon rings in water to obtain solution A; dissolve patinol in water to obtain solution B; mix solution A with solution B to control seven-membered melon rings and patinol The molar ratio is 1:0.5, react at room temperature to obtain a probe solution, dilute the probe solution with neutral water to a concentration of 2.0×10 -5 mol / L, to obtain the standard probe solution;

[0047] 2) L-Phenylalanine recognition: Add the solution to be tested dropwise into the standard probe solution, let it stand for 5s, then measure the fluorescence spectrum at a fixed excitation wavelength of 342nm, and draw the fluorescence intensity at the excited laser wavelength When the fluorescence intensity at 493nm is quenched, it indicates that the solution to be tested contains L-phenylalanine, otherwise it does not.

Embodiment 3

[0048] Embodiment 3: A method for identifying L-phenylalanine, the steps are as follows:

[0049] 1) Preparation of standard probe solution: Dissolve seven-membered melon rings in water to obtain solution A; dissolve patinol in water to obtain solution B; mix solution A with solution B to control seven-membered melon rings and patinol The molar ratio is 1: 2, react at room temperature to obtain a probe solution, dilute the probe solution with neutral water to a concentration of 2.0×10 -5 mol / L, to obtain the standard probe solution;

[0050] 2) L-phenylalanine recognition: Add the solution to be tested dropwise into the standard probe solution, let it stand for 15s, then measure the fluorescence spectrum at a fixed excitation wavelength of 342nm, and draw the fluorescence intensity at the excited laser wavelength When the fluorescence intensity at 493nm is quenched, it indicates that the solution to be tested contains L-phenylalanine, otherwise it does not.

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Abstract

The invention discloses a method for identifying L-phenylalanine. The method is characterized by using a fluorescent probe as an identification reagent; and the fluorescent probe is prepared by a seven-membered cucurbituril or an eight-membered cucurbituril and fibrauretin. The method disclosed by the invention has the advantages of simple identification method, low identification cost and high selection unicity.

Description

technical field [0001] The invention relates to a recognition method of L-phenylalanine, in particular to a fluorescent probe recognition method of L-phenylalanine. Background technique [0002] Amino acids play an extremely important role in the growth and development of organisms: 1. It is a constituent part of human tissue; 2. It constitutes various substances in the human body; 3. It supplies heat; Carrier, plays the role of transport; 6. Oxidation function. Amino acids are the basic components of proteins. Intake of amino acids is the only way for the human body to obtain nitrogen sources. After the human body absorbs amino acids, a part is directly used to synthesize proteins, and a part is oxidized and decomposed. The nitrogen-containing part is used to synthesize other essential amino acids, and part is used as The energy is broken down and excreted in the form of urea. [0003] L-phenylalanine (English name: L-Phenylalanine) is one of the essential amino acids. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/33C07D455/03C09K11/06
CPCG01N21/6402G01N21/6428G01N21/6486G01N21/33C07D455/03C09K11/06G01N2021/6432C09K2211/1029C09K2211/1007C09K2211/1011
Inventor 肖昕单培辉范颖邓鑫玉
Owner GUIZHOU UNIV
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