Method for identifying rice and dry rice
A technology of rice and identification value, applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of water and terrestrial ecotype confusion of cultivated rice germplasm resources, and problems of germplasm resource utilization and research, etc. Good promotion and application prospects, simple identification method, fast and efficient effect
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[0046] Example 1:
[0047] Five pairs of SSR molecular markers were used to identify rice and upland rice ecotypes in 30 domestic cultivated rice germplasm resources. See Table 4 for specific experimental materials.
[0048] Table 4. 30 local rice and upland rice materials from different regions of my country.
[0049]
[0050] Extract DNA samples from any organ or tissue of rice plants (including seeds, seedlings, adult plants, stems, leaves, etc.), and perform polymerase chain amplification on the sample DNA. The polymerase chain amplification system used in the present invention is conventional The three-primer PCR system, PCR machines produced by any company and reagents produced and synthesized by any biological reagent company can be used to achieve the purpose. The PCR reaction conditions and procedures in the present invention are as follows (20μl system):
[0051] PCR reaction conditions:
[0052] 1) 10×Taq buffer (containing 25mM MgCl2) 2μl
[0053] 2) 10mM dNTP Mixture 0.4...
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[0073] Example 2:
[0074] Five pairs of SSR molecular markers were used to identify rice and upland rice ecotypes in 20 international cultivated rice germplasm resources. See Table 6 for specific experimental materials.
[0075] Table 6. 20 local rice and upland rice materials from different regions of the world.
[0076]
[0077] Extract DNA samples from any organs or tissues of rice plants (including seeds, seedlings, adult plants, stems, leaves, etc.), and perform polymerase chain amplification on the sample DNA. The polymerase chain amplification system used in the present invention is a conventional three-primer PCR system. PCR machines produced by any company and reagents produced and synthesized by any biological reagent company can be used to achieve the purpose. The PCR reaction conditions and procedures are as follows (20μl system):
[0078] PCR reaction conditions
[0079] 1) 10×Taq buffer (containing 25mM MgCl2) 2μl
[0080] 2) 10mM dNTP Mixture 0.4μl
[0081] 3) 20μM pr...
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